JPH0790184B2 - Centrifuge container assembly - Google Patents

Description

Description: TECHNICAL FIELD The present invention relates to a centrifuge container assembly for a continuous centrifuge, and more particularly, to a continuous centrifuge suitable for washing blood of patients with hematological disorders. The present invention relates to a centrifuge container assembly for a device.

[Prior Art and its Problems] Patients with hematological disorders, such as jaundice, have dirty plasma. In the treatment of such jaundice, blood is continuously collected from the patient and the collected blood is continuously collected. It has been practiced to separate the contaminated plasma by centrifuging and dispose of it, and then add the plasma preparation to blood other than plasma and return it to the patient's body.

A continuous blood centrifuge is used for such separation and washing of blood. In such a centrifuge, a patient and a centrifuge container are separated into a stationary system and a rotating system. Nevertheless, it is an absolute requirement that the entire system of blood entry / exit tubes (hereinafter referred to as blood tubes) that connect these sections be a completely closed system. This is because if the system is imperfect, bacteria and the like will be mixed into the blood and pose a risk to the patient. It is also required that the blood tube has a long breaking life. If the life is short, the blood tube will be damaged during blood washing, bacteria and other substances will be mixed into the blood, and a large amount of blood will flow out from the patient's body, thus putting the patient at risk. Furthermore, during such blood cleansing treatment, the patient is given a certain amount of pain, and therefore, it is necessary to complete the blood cleansing in a short time. Therefore, high separation efficiency is required. Here, as a continuous centrifugal separator capable of realizing a completely closed system, for example, there is one shown in JP-A-51-120470. This structure and mechanism are briefly shown in FIGS. 6 and 7.

In FIG. 6, reference numeral 71 indicates a rotator for centrifugation, in which a centrifuge container 72, an injection nozzle 73 for injecting blood into the centrifuge container, and a centrifuge container 72 are centrifuged. It has a discharge nozzle 74 for discharging the liquid. The injection nozzle 73 and the discharge nozzle 74 extend downward from the rotating body 71 and are rotatably held at one end of the tube twist prevention rotary table 75 by a holder 76 or the like. It is rotatably held by a holder 78 at one end of an arm 77 extending upward, and is connected to the outside through a protective tube 79 fixed to an upper fixing member 80 located on the rotation center axis of the rotating body 71. . In this state, the tube twist prevention table 75
Is rotated in the same direction as the rotating body 71 and at a rotation speed of, for example, 1/2, the protective tube 79 and the injection / discharge nozzle 73 are rotated.
As for 74 and 74, no twist occurs as a whole even when the rotating body rotates. However, in the holder 76 and the holder 78, and in the curved portion of the tube, the tube is temporarily twisted, causing torsional vibration, and unnecessary force acts on the tube to destroy the tube. There was an occasion.

Further, FIG. 7 shows a cross-sectional top view of the rotating body 71 shown in FIG. Here, the centrifugation container 72 is symmetrically arranged on the rotating body 71, and the injection nozzle 73 and the discharge nozzle group 74 are provided so as to extend in the same direction. Therefore, the blood injected from the injection nozzle 73 may be directly discharged from the discharge nozzle 74 and may not be sufficiently separated, and sufficient separation efficiency may not be obtained. Therefore, realization of safe and high separation efficiency of continuous centrifugation of blood, etc. could not be expected.

[Problems to be Solved by the Present Invention] The present invention can eliminate or eliminate the above-mentioned problems, that is, the incompleteness of blood separation, the lengthening of the separation time, and the occurrence of torsional vibration that causes tube breakage. A centrifuge container assembly for a continuous centrifuge is provided.

[Means for Solving the Problems] The present invention relates to a centrifuge container in which a container having a substantially rectangular vertical cross section is provided with a projecting portion in the center of the side surface thereof, and a communication pipe for communicating both ends of the container is provided. 1; an injection nozzle 5 provided in the end direction of the container, a first discharge nozzle 6 provided in the direction opposite to the injection nozzle 5, a second discharge nozzle 7 provided in the direction of the protrusion of the separation container 1, and Centrifugal container assembly comprising a nozzle group including a third discharge nozzle 8 provided in the rotation axis direction of the separation container 1, a tube group corresponding to the nozzle group, and means for integrally holding the tube group. Therefore, the above problems are effectively solved.

Embodiments Embodiments according to the present invention will be described below with reference to FIGS. 1 to 5, but the present invention is not limited to these.

FIG. 1 shows the overall construction of a continuous centrifugation container assembly according to the present invention. This continuous centrifuge container assembly includes a centrifuge container 1 and an injection nozzle 5 provided inside the container 1 for injecting, for example, blood into the centrifuge container 1, or
The first to discharge the separated blood component from the centrifuge container 1
-The 3rd discharge nozzle 6-8 (refer to Drawings 3-5), the injection tube 15 connected with the injection nozzle 5, and the discharge tube 16 connected with each of the 1st-3rd discharge nozzles 6-8. ~ 18
And, for example, a tube member 3 rotatably held by a holder 76 provided on a tube twist prevention table 75 as shown in FIG. 6, and a tube between the centrifugation container 1 and the tube member 3. Spring 2 that positively removes the twist of 15-18,
Tube protector 9 that integrally holds tubes 15-18
And a coating tube 12 that further covers the tubes 15 to 18,
The tube fixing bush 11 is integrated at the inlets of the tubes 15 to 18 into the coating tube.
Here, each component will be described below.

(A) Centrifuge vessel 1 Fig. 3 shows the centrifuge vessel 1 according to the present invention A-A in Fig. 1.
A sectional view is shown, and FIG. 4 shows a sectional view taken along the line BB of FIG. 3, and FIG. 5 shows a sectional view taken along the line CC of FIG. As shown in FIGS. 3 to 5, the centrifuge container 1 is provided with a protrusion C at the center of the side surface substantially at right angles to the longitudinal direction of the container 1, and at both ends a and b of the container 1. A circular communication pipe 14 is provided for communicating both ends.

An injection nozzle 5 for injecting blood into the container 1 and a first ejection nozzle 6 for ejecting mainly red blood cells of the infused blood to the outside of the container.
Means that openings ₁ and d ₂ are provided at positions extending toward the end of the container 1 and in directions opposite to each other. Further, the second discharge nozzle 7 for discharging mainly white blood cells, platelets, etc. of the injected blood is provided with openings d ₃ and d _{4 at} a position extending in a direction substantially perpendicular to the discharge nozzle 6. .

Further, the injection nozzle 5 and the first to third discharge nozzles 6 to 8 are used.
In addition to the positional relationship of the openings d ₃ of the second exhaust nozzle 7 or
If the horizontal distance between d ₄ and the center of the shaft 4 is approximately half the radius of the container 1, white blood cells and platelet components can be separated with high efficiency and efficiently.

The nozzles 5 to 8 are connected to the tubes 15 to 18 through the shaft 4 as shown in FIG.

It is convenient to manufacture the centrifuge container 1 and the communication tube 14 by using a transparent acrylic resin or the like.

Here, the horizontal distance from the center of rotation of the openings of the injection nozzle 5 and the first to third discharge nozzles 6 to 8 differs depending on the specific gravity of the sample to be injected, for example, blood, and the content of the blood component to be centrifuged. Therefore, it is preferable that the distance can be freely changed. In this embodiment, for example, the distance from the center of rotation of the opening d ₁ of the injection nozzle 5 is 40 mm,
The _second discharge nozzle for discharging the red blood cell component (the specific gravity of red blood cells is the largest compared to other components), the distance between the opening d ₂ of the discharge nozzle 6 and the center of rotation is 60 mm, and platelets, white blood cell components, etc. In the third discharge nozzle 8 for discharging the plasma component (the plasma component has the smallest specific gravity as compared with other components), the distance between the d ₃ and the rotation center of 7 is 30 mm, and it is upward along the rotation center axis. An opening d ₅ was provided in the above and the same distance was set to 0 mm. The height of each nozzle 5-8 from the bottom of the centrifuge container also depends on the specific gravity of blood and components to be centrifuged, but in this embodiment, the injection nozzle 5 and the first and second discharge nozzles 6 and 7 are The height is the same as the center height of the communication pipe 14, and the third discharge nozzle 8 is directed upward from the center of rotation as described above.

Here the injection nozzle 5 and not toward the opening d ₁ to d ₅ of the first to third discharge nozzles 6-8 on opposite direction to the same direction, one side (the central axis of rotation of the centrifuge vessel This is to prevent the centrifugal separation from being carried out only in (border) and to carry out the whole centrifugation container, which enables more separations in one centrifugation operation. , The first to the injected blood is not separated
Direct discharge from the third discharge nozzles 6 to 8 is also prevented.

Next, the operation of the communication pipe 14 will be described. Blood is injected into the centrifuge container from the injection nozzle 5, and the centrifuge container 1 is, for example, rotated at high speed [about 3000 rpm (600-1200G)].
Then, the plasma component having a low specific gravity is collected in the central part of the container, and the red blood cell component having a high specific gravity is centrifugally collected in the peripheral part of the container. Then, the erythrocyte component moves to the opposite side of the centrifuge container through the communication pipes 14 provided at both ends (peripheral edge) of the container, and passes through the first discharge nozzle 6 for ejecting the erythrocyte component. It is discharged to the outside. Here, since the centrifuge container 1 is provided with a projecting portion to improve the centrifugal separation performance, and both ends a and b of the centrifuge container 1 are connected by the communication pipe 14, it is necessary. It is possible to discharge various blood components from the centrifuge container.

(B) Barrel 3 and spring 2 The nozzle 5 for injecting blood into the centrifugal container 1 and the nozzles 6 to 8 for discharging blood components as described above extend below the center of rotation of the centrifugal container. As shown in FIG. 6, it is rotatably held by a holder 76 provided on the twist prevention table 75. Here, when the centrifuge container rotates,
In the structure shown in Fig. 6, the entire pipe system (ABCD)
Does not cause twist in the pipe, but partially causes twist in AB, BC, and CD, for example.
Therefore, the twist that partially occurred is removed, and the twist is
The tube should not be affected.
Especially between A and B, because the tube is curved and the number of rotations of the centrifuge container and the anti-twist table (a part of the tube is held on it) is different, There is a large amount of twist. The cylinder 3 is fixed to the tubes 15 to 18, and the fixing method is, for example, as shown in FIG.
It is preferable to arrange and fix them so that they are more equidistant and equiangular. These tubes here 15-18
A spring 2 is provided so as to surround the cylinder 1, one end of which is fixed to the barrel 3 and the other end of which is fixed to the centrifugation container 1. Further, since the barrel 3 is made rotatable with the holder 76, it is more preferable that the holder be a bearing and the barrel 3 be shaped and structured so as to be fixed to the inner ring of the bearing. The spring 2 is set in a normal state when the centrifuge container 1 is not rotating. Here, when the centrifugation container 1 rotates,
Twist occurs in tubes 15-18 between AB,
This twist also acts on the spring 2 provided between the barrel 3 and the centrifuge vessel 1. However, when the spring 2 is twisted, its reaction (which works to return to the twist) positively removes the twist between AB and AB.
Torsional force is not applied to the tube between them. here
A metal box and a spring are provided only between AB, but BC
, Or even between CDs.

(C) Pipe protection body 9, coating tube 12, tube fixing bush 11, and coating tube protection body 13 Since a large centrifugal force acts on tubes 15 to 18 between BCDs, a large amount of twisting is removed between BCDs. It is not preferable to provide a mechanism, for example, a metal bar and a spring provided between AB. Therefore, in the embodiment according to the present invention, a tube protector 9 is provided between BCs to increase the torsional strength of the tube, and C-
Between the sections D, the covering tube 12 and the tube fixing bush 11 are provided in order to integrate the tubes 15 to 18 and further increase the torsional strength thereof.

Here, as shown in FIG. 1, the number of tube protectors 9 is basically two, but good results were obtained with any number between 1 and 20. The tubes 15 to 18 pass through the tube protector 9 and are fixed in the arrangement (similar to the fixing to the tube) as shown in FIG. 2, for example.

Furthermore, the fixation of the tube protector 9 and the tubes 15-18 is
Installed between BC, that is, in the centrifuge,
It is more preferable to carry out the process with a centrifuge container installed.

Next, between the CDs, the tubes 15 to 18 are substantially straight, so that the tubes 15 to 18 are integrated so that the covered tube 12
Tubes 15 to 18 are put in the tube, and the covered tube 12 is fixed to the tubes 15 to 18 by a covered tube protector 13.
Here, the covering tube 12 and the covering tube protector 13 may be made of vinyl chloride, silicone resin, or the like. Furthermore, the tube
15-18, in the inlet portion entering the coating tube 12,
It is preferable to provide a tube fixing bush 11 for integrally fixing the tubes 15 to 18. Here, the fixing bush 11 may be made of the same material as the covering tube protector 13.
As described above, since the tubes 15 to 18 are integrated between the BCDs, the rigidity is increased and the strength against twisting is increased, so that the tube breakage at this portion can be prevented.

As described above, in the entire tube system, the breakage due to the twisting of the tube can be prevented by the positive removal of the twist between AB and the increase of the strength of the tube between BCD.

It is obvious that those skilled in the art can make various modifications and improvements based on the above description.

[Effects of the Invention] The present invention provides a centrifuge container assembly for a continuous centrifuge, which has high sample separation ability and separation efficiency, and is safe and has a long life.

[Brief description of drawings]

1 is an explanatory view of the overall configuration of a centrifuge container assembly for a continuous centrifuge according to the present invention; FIG. 2 is an enlarged view of a ZZ cross section of FIG. 1; and FIG. 3 is in accordance with the present invention. FIG. 3 is a cross-sectional view taken along the line AA of the centrifugation container of the example, in which the arrow indicates the blood flow direction. FIG. 4 is a sectional view taken along the line BB of FIG. 3; FIG. 5 is a sectional view taken along the line CC of FIG. 3; FIG. 6 is a view for explaining a conventional centrifuge container assembly; and FIG. It is a top view of the conventional centrifugation container.

 ─────────────────────────────────────────────────── ─── Continuation of front page (56) References JP-A-61-245855 (JP, A)

Claims (1)

[Claims] 1. A centrifuge container 1 provided with a projecting portion at the center of a side surface of a container having a substantially rectangular vertical cross-section, and a communication pipe for communicating both ends of the container; and an end of the container. The injection nozzle 5 provided in the direction of the part, the first discharge nozzle 6 provided in the direction opposite to the injection nozzle 5, the second discharge nozzle 7 provided in the direction of the protrusion of the separation container 1, and the rotation axis direction of the separation container 1. A centrifugal vessel assembly comprising: a nozzle group including the third discharge nozzle 8 provided in the above; a tube group corresponding to the nozzle group; and means for integrally holding the tube group.