JPH078229B2 - Cell culture tank - Google Patents

Cell culture tank

Info

Publication number
JPH078229B2
JPH078229B2 JP62336366A JP33636687A JPH078229B2 JP H078229 B2 JPH078229 B2 JP H078229B2 JP 62336366 A JP62336366 A JP 62336366A JP 33636687 A JP33636687 A JP 33636687A JP H078229 B2 JPH078229 B2 JP H078229B2
Authority
JP
Japan
Prior art keywords
filter
culture
cell
cell culture
culture tank
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP62336366A
Other languages
Japanese (ja)
Other versions
JPH01174376A (en
Inventor
栄太郎 熊沢
隆 真野
晶彦 村上
正次 上田
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Snow Brand Milk Products Co Ltd
Original Assignee
Snow Brand Milk Products Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Snow Brand Milk Products Co Ltd filed Critical Snow Brand Milk Products Co Ltd
Priority to JP62336366A priority Critical patent/JPH078229B2/en
Publication of JPH01174376A publication Critical patent/JPH01174376A/en
Publication of JPH078229B2 publication Critical patent/JPH078229B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • C12M27/08Stirrer or mobile mixing elements with different stirrer shapes in one shaft or axis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04Filters; Permeable or porous membranes or plates, e.g. dialysis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10Perfusion
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/02Filters

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Description

【発明の詳細な説明】 (産業上の利用分野) 本発明は細胞を培養する培養槽において、細胞が消費す
る酸素を大量に供給することにより高細胞濃度の培養を
維持し、同時に高密度培養に不可欠な培養液のかん流を
容易にする細胞培養槽に関する。
DETAILED DESCRIPTION OF THE INVENTION (Field of Industrial Application) The present invention maintains a high cell concentration culture by supplying a large amount of oxygen consumed by cells in a culture tank for culturing cells, and at the same time high density culture. The present invention relates to a cell culture tank that facilitates perfusion of a culture solution, which is essential for cell culture.

(従来の技術) 細胞培養は、一般的にタンク培養で行われており、接着
担体を必要とする接着性細胞の場合にあってはローラビ
ン、マルチプレート、マイクロキャリア等の方法が利用
されている。
(Prior Art) Cell culture is generally performed in tank culture, and in the case of adhesive cells requiring an adhesive carrier, methods such as roller bin, multi-plate, and microcarrier are used. .

そして、これらの方法の中でも、マイクロキャリアを培
養液中に浮遊させて細胞培養するマイクロキャリア方式
は代表的な大量培養の方法である。
Among these methods, the microcarrier method in which the microcarriers are suspended in the culture medium and the cells are cultured is a typical mass culture method.

しかしながら、通常のマイクロキャリア方式において行
われているような上面通気による酸素供給にはその供給
量などに限界があり、高細胞濃度の培養を維持すること
が出来ないという欠点がある。
However, there is a limitation in the amount of oxygen supplied by top surface ventilation, which is performed in the usual microcarrier system, and there is a drawback that it is not possible to maintain high cell culture.

そこで、この欠点を解決する手段として、従来特開昭62
−122581号に示すような培養槽内に酸素を直接供給する
手段や、特開昭54−92687号に示すような撥水性膜を介
して分圧差により培養液中に酸素を溶解させる手段等が
提案されている。
Therefore, as a means for solving this drawback, there is a conventional Japanese Patent Laid-Open No.
-122581, a means for directly supplying oxygen into the culture tank, a means for dissolving oxygen in the culture medium by a partial pressure difference through a water-repellent membrane as shown in JP-A-54-92687, and the like. Proposed.

また、高密度培養のためには、培養液を連続的、もしく
は定期的にかん流する(古い培養液を取り除いて新しい
培養液を注入する)必要があるが、その際、接着担体と
分離して培養液のみを抜き取るために、培養液の排出管
の端部に焼結金属フィルターやステンレスフィルターな
どを取り付ける方法が従来採られている。
In addition, for high-density culture, it is necessary to continuously or regularly perfuse the culture medium (remove old culture medium and inject new culture medium), but at that time, separate it from the adhesive carrier. In order to extract only the culture solution by using the method, a method of attaching a sintered metal filter, a stainless filter or the like to the end of the culture solution discharge pipe has been conventionally adopted.

(発明が解決しようとする問題点) しかしながら、上述の特開昭62−122581号に示すものは
細胞が接着した接着担体の浮遊する培養液に対して空気
及びまたは酸素を直接噴気するために、培養液中に大き
な攪乱流を生じ、剪断力によるダメージを細胞に与える
という不都合や、接着担体が気泡によって押し上げられ
て培養槽の上部内壁やセンサー等に付着し、培養液中に
浮遊しなくなって培養に支障を来すという不都合があ
り、また、特開昭54−92687号に示すものは大量の酸素
の供給が行えず、例えば組換え体細胞のような酸素消費
量の多い培養には供給が十分でないという不都合があ
る。
(Problems to be Solved by the Invention) However, in the above-mentioned JP-A-62-122581, in order to directly blow air and / or oxygen to the culture medium in which the adhesive carrier to which cells adhere is suspended, Inconvenience that a large turbulent flow is generated in the culture solution and damage to cells by shearing force is caused, and the adhesive carrier is pushed up by bubbles and adheres to the inner wall of the upper part of the culture tank, the sensor, etc. and does not float in the culture solution. There is an inconvenience that it interferes with culturing, and the one described in JP-A-54-92687 cannot supply a large amount of oxygen, and is supplied to a culture with a large oxygen consumption such as a recombinant cell. Is not enough.

また、培養液のかん流を行うに際しては、排出管の端部
に設けられたフィルターに細胞担体が目詰まりする等の
問題がある。
In addition, when the culture solution is perfused, there is a problem that the filter provided at the end of the discharge tube is clogged with the cell carrier.

(問題を解決するための手段) 本発明は以上の問題を解決し、細胞にダメージを与える
ことなく培養液中に細胞が消費する酸素を大量に供給す
ることにより高細胞濃度の培養を維持し、同時に高密度
培養に不可欠な培養液のかん流を容易に行える細胞培養
槽を得ることを技術的課題とするものであり、この課題
を解決するために、細胞培養槽の一部に培養液は透過す
るが細胞の接着担体は透過しないフィルターによって隔
離される領域を設けるとともに、該領域内に酸素及びま
たは空気を供給する導管を配設して培養液中に酸素を供
給するようにして細胞培養槽を構成するようにした。
(Means for Solving Problems) The present invention solves the above problems and maintains a high cell concentration culture by supplying a large amount of oxygen consumed by cells to a culture solution without damaging the cells. At the same time, it is a technical issue to obtain a cell culture tank that can easily perfuse the culture solution, which is essential for high-density culture, and in order to solve this problem, a part of the cell culture tank contains a culture solution. A region that is isolated by a filter that allows cells to pass through but not the cell's adhesive carrier is provided, and a conduit for supplying oxygen and / or air is provided in the region to supply oxygen to the culture medium. The culture tank was constructed.

なお、以上のような細胞培養槽を構成する上において、
フィルターを介しての培養槽液の移動が激しくなると、
フィルターの外面に細胞接着担体が付着する場合もある
が、この付着力は弱いものであるのでフィルターを回転
するものや振動するものなどにすることによって容易に
その付着した細胞接着担体を除去することが出来るもの
である。
In addition, in configuring the cell culture tank as described above,
When the movement of the culture tank liquid through the filter becomes vigorous,
The cell adhesive carrier may adhere to the outer surface of the filter, but since this adhesive force is weak, it is easy to remove the adhered cell adhesive carrier by using a filter that rotates or vibrates. Can be done.

(作用) 本発明は以上のように構成したので、フィルターで隔離
された領域内において培養液に酸素や空気を供給するこ
とにより、この領域の外に浮遊する細胞に対し攪乱によ
る剪断力のダメージを与えることがなく、この領域内に
おいてじゅうぶんに酸素を得た培養液がフィルターの側
面全体から外側の培養液中に透過していくことになる。
(Operation) Since the present invention is configured as described above, by supplying oxygen or air to the culture solution in the area isolated by the filter, the cells floating outside the area are damaged by the shearing force due to the disturbance. In this region, the oxygen-containing culture solution permeates into the outer culture solution from the entire side surface of the filter.

また、フィルターは培養液は透過するが細胞が付着する
接着担体は透過しないものを使用するようにしたので、
この領域内は接着担体と分離した培養液のみで満たされ
ることになり、フィルターを使用せずに培養液のかん流
を行うことも可能になる。
In addition, since the filter used is one that is permeable to the culture solution but not to the adhesive carrier to which cells are attached,
This area will be filled only with the culture solution separated from the adhesive carrier, and it becomes possible to perfuse the culture solution without using a filter.

なお、培養液のかん流はフィルター内に限定されるもの
ではない。
The perfusion of the culture solution is not limited to the inside of the filter.

(実施例) 以下、本発明の実施例を図面について説明する。第1、
2図は、本発明にかかる細胞培養槽を示すものであっ
て、槽本体(A)の内部にはマイクロキャリア等の接着
担体(a)が浮遊する培養液(1)が液面(1′)の高
さまで充填されている。
Embodiment An embodiment of the present invention will be described below with reference to the drawings. First,
FIG. 2 shows a cell culture tank according to the present invention. Inside the tank body (A), a culture solution (1) in which an adhesive carrier (a) such as a microcarrier floats is a liquid surface (1 ′). ) Is filled up to the height.

(2)はその側面(2′)及び底面(2″)によって領
域(B)を仕切っているフィルターであり、このフィル
ター(2)は培養液は透過するが細胞が付着する接着担
体(a)は透過しないもの、すなわち接着担体としての
マイクロキャリアーなどを透過しない程度の細孔が多数
穿たれているものであって、培養液(1)は自由に透過
する能力をもったフィルターから成っている。そして、
領域(B)内には接着担体(a)が浮遊しない培養液
(1)のみが充填されている。なお、フィルター(2)
の材質は、気体を吸着しないもの、例えばガラス、焼結
金属、セラミックス、紙、プラスチック、ステンレスメ
ッシュ等を用いることが可能であるが、これに限定され
るものではない。
(2) is a filter that partitions the region (B) by its side surface (2 ') and bottom surface (2 "). This filter (2) is an adhesive carrier (a) through which the culture solution permeates but the cells adhere. Is a non-permeable one, that is, a large number of pores are formed to the extent that it does not penetrate a microcarrier as an adhesive carrier, and the culture solution (1) is composed of a filter having the ability to freely permeate. And
The region (B) is filled only with the culture solution (1) in which the adhesive carrier (a) does not float. The filter (2)
As the material, a material that does not adsorb gas, for example, glass, sintered metal, ceramics, paper, plastic, stainless mesh, or the like can be used, but the material is not limited to this.

領域(B)は上方が槽本体(A)内の気体層部分(3)
において解放する開口部(B′)を有しており、この開
口部(B′)からは酸素もしくは空気を供給するための
導管(4)と、培養液(1)のかん流をするために培養
液(1)の供給と排出を行う供給排出管(5)とが領域
(B)に対して挿入されている。
The upper part of the region (B) is the gas layer part (3) in the tank body (A).
In order to perfuse the culture solution (1) with a conduit (4) for supplying oxygen or air from this opening (B '). A supply / discharge pipe (5) for supplying and discharging the culture solution (1) is inserted into the region (B).

その他、(6)、(7)は培養液(1)を攪拌する攪拌
羽根であって、モーター(8)の駆動により攪拌羽根
(6)および攪拌羽根(7)が作動するが、攪拌羽根
(6)は勾配をきつくとって攪拌力を大きくし、逆に攪
拌羽根(7)は勾配を緩くとって攪拌力を小さなものと
し、細胞に剪断力によるダメージを与えないようになっ
ている。
In addition, (6) and (7) are stirring blades for stirring the culture solution (1). The stirring blade (6) and the stirring blade (7) are actuated by the drive of the motor (8). In 6), the stirring force is increased by increasing the gradient, and conversely, by the stirring blade (7), the stirring force is decreased by decreasing the gradient so that the cells are not damaged by the shearing force.

従って、以上のように構成された本発明細胞培養槽を利
用することにより、領域(B)内において、導管(4)
から培養液(1)に対して酸素が供給されて培養液
(1)に酸素が溶け込み、他方導管(4)から領域
(B)内に供給されたにもかかわらず培養液(1)に溶
けなかった気体は上方に解放する開口部(B′)から槽
外へ排出される。
Therefore, by using the cell culture tank of the present invention configured as described above, in the region (B), the conduit (4)
Oxygen is supplied to the culture solution (1) from the medium and the oxygen is dissolved in the culture solution (1), while it is dissolved in the culture solution (1) even though it is supplied from the conduit (4) into the region (B). The remaining gas is discharged to the outside of the tank through the opening (B ′) that opens upward.

そして、この領域(B)内において気体による攪乱流と
攪拌羽根(6)による攪拌流によって十分に酸素が溶け
込んだ培養液(1)はフィルター(2)の側面(2′)
及び底面(2″)全体から外側の培養液中に透過してい
くことになり、領域(B)の外に浮遊する細胞に対し
て、酸素を供給する際に生じる攪乱流による剪断力力の
ダメージを与えることがなく、しかも、接着担体(a)
が培養槽(A)の上部内壁や図示しないセンサー等に付
着することによる不都合も解消されることになる。
Then, in this region (B), the culture solution (1) in which oxygen is sufficiently dissolved by the turbulent flow by the gas and the agitating flow by the agitating blade (6) is the side surface (2 ') of the filter (2).
And the entire bottom surface (2 ″) permeate into the culture medium outside, and to the cells floating outside the area (B), the shear force due to the turbulent flow generated when oxygen is supplied is Adhesive carrier (a) without causing damage
The inconvenience caused by the adhering to the inner wall of the upper part of the culture tank (A), a sensor (not shown), etc. will be eliminated.

また、フィルター(2)は培養液(1)は透過するが接
着担体(a)は透過しないものを使用しているので、こ
の領域(B)内には接着担体(a)と分離した培養液
(1)のみが液面(1′)まで満たされることになり、
領域(B)内に配設された、先端にフィルターを有さな
い供給排出管(5)によって、細胞担体(a)の目詰ま
りを気にせずに培養液(1)のかん流を行うことが可能
となる。
In addition, since the filter (2) uses a medium that permeates the culture medium (1) but does not permeate the adhesive carrier (a), the culture medium separated from the adhesive carrier (a) in this region (B). Only (1) will be filled up to the liquid level (1 '),
The perfusion of the culture solution (1) is performed without worrying about the clogging of the cell carrier (a) by the supply and discharge pipe (5) provided in the region (B) and having no filter at the tip. Is possible.

なお、以上のような細胞培養槽(A)を構成する上にお
いて、フィルター(2)を介しての培養液(1)の移動
が激しくなると、フィルター(2)の外面に細胞接着担
体(a)が付着する場合もあるが、この付着力は弱いも
のであるのでフィルター(2)を回転するものや振動す
るものなどにすることによって容易にその付着した細胞
接着担体(a)を除去することが出来る。
In the above cell culture tank (A), when the movement of the culture solution (1) through the filter (2) becomes vigorous, the cell adhesive carrier (a) is attached to the outer surface of the filter (2). However, since the adhesive force is weak, it is possible to easily remove the attached cell adhesive carrier (a) by using a filter (2) that rotates or vibrates. I can.

第3図は細胞培養槽(A)の上部にモーター(10)を装
着し、駆動軸(11)の下端のピニオンギヤ(12)とフィ
ルター(2)の外周に配設したリングギヤ(13)とを噛
合せしめることにより、モーター(10)の駆動でフィル
ター(2)を回転させるようにしたものであるが、フィ
ルター(2)を回転させる手段としては、攪拌羽根
(6)、攪拌羽根(7)を作動させているモーター
(8)の駆動力を利用する方法なども考えられる。
FIG. 3 shows that the motor (10) is mounted on the upper part of the cell culture tank (A), and the pinion gear (12) at the lower end of the drive shaft (11) and the ring gear (13) arranged on the outer periphery of the filter (2) are installed. The filter (2) is rotated by driving the motor (10) by engaging with each other. As a means for rotating the filter (2), a stirring blade (6) and a stirring blade (7) are used. A method of utilizing the driving force of the operating motor (8) is also conceivable.

第4図は細胞培養槽(A)の上部に機械的もしくは電気
的手段による振動発信装置(14)を装着し、その作動杆
(15)の下端をフィルター(2)に当接せしめることに
より、振動発信装置(14)でフィルター(2)を振動さ
せるようにしたものである。
FIG. 4 shows that a vibration transmission device (14) by mechanical or electrical means is attached to the upper part of the cell culture tank (A), and the lower end of its operating rod (15) is brought into contact with the filter (2). The vibration transmission device (14) vibrates the filter (2).

第5図はフィルター(2)の側面(2′)を波型に形成
したものであり、フィルター(2)の表面積を増加させ
て培養液(1)の透過量が多くなるようにした一例を示
すものである。
FIG. 5 shows a corrugated side surface (2 ′) of the filter (2), which is an example in which the surface area of the filter (2) is increased to increase the permeation amount of the culture solution (1). It is shown.

また、本発明は細胞培養槽の一部にフィルターによって
隔離される領域を設け、その領域内に導管を配設したも
のであれば良く、例えば第6図に示すように培養槽
(A)の上部内壁に沿った領域(B′)を設け、この領
域(B′)内に導管(4′)を配設するようにしても良
く、その他任意の具体的手段を採り得るものである。
Further, the present invention is only required to provide a region isolated by a filter in a part of the cell culture tank and to arrange a conduit in the area, for example, as shown in FIG. A region (B ') along the upper inner wall may be provided, and the conduit (4') may be arranged in this region (B '), and any other specific means can be adopted.

(発明の効果) 以上、本発明によれば、従来同様の簡単な細胞培養槽に
おいて、培養槽内に浮遊する細胞にダメージを与えずに
細胞が消費する酸素を大量に供給することができるの
で、従来困難であった高細胞濃度の培養が容易に出来る
ようになり、しかも培養を長期間に渡って連続的に行う
ことが可能であるという優れた効果を奏することにな
る。
(Effects of the Invention) As described above, according to the present invention, a large amount of oxygen consumed by cells can be supplied in a simple cell culture tank similar to the conventional one, without damaging the cells floating in the culture tank. Thus, it is possible to easily perform the culture of high cell concentration, which was difficult in the past, and further, it is possible to achieve the excellent effect that the culture can be continuously performed for a long period of time.

また本発明のものは、フィルターを回転するものや振動
するものなどにすることにより、フィルターに付着した
細胞接着担体を容易に除去することができるという効果
も有している。
Moreover, the thing of this invention also has the effect that a cell adhesive carrier adhering to a filter can be easily removed by making a filter rotate or vibrate.

【図面の簡単な説明】[Brief description of drawings]

第1、2図は本発明にかかる細胞培養槽の斜視図と断面
図、第3図は同細胞培養槽においてフィルターを回転す
るように構成したものの断面図、第4図は同細胞培養槽
においてフィルターを振動するように構成したものの断
面図、第5図は側面を波型に形成したフィルターの斜視
図、第6図は本発明の変形実施例にかかる細胞培養槽の
斜視図をそれぞれ現す。 (A)……培養槽 (B)……領域 (a)……接着担体 (1)……培養液 (2)……フィルター (3)……気体層部分 (4)……導管 (5)……供給排出管 (6)、(7)……攪拌羽根 (8)……モーター
1 and 2 are a perspective view and a cross-sectional view of a cell culture tank according to the present invention, FIG. 3 is a cross-sectional view of a cell culture tank configured to rotate a filter, and FIG. 4 is a cross-sectional view of the cell culture tank. FIG. 5 is a cross-sectional view of a filter configured to vibrate, FIG. 5 is a perspective view of a filter having a corrugated side surface, and FIG. 6 is a perspective view of a cell culture tank according to a modified embodiment of the present invention. (A) …… culture tank (B) …… area (a) …… adhesive carrier (1) …… culture medium (2) …… filter (3) …… gas layer (4) …… conduit (5) …… Supply and discharge pipes (6), (7) …… Stirring blade (8) …… Motor

───────────────────────────────────────────────────── フロントページの続き (72)発明者 上田 正次 埼玉県川越市今福1672―1 メゾンむさし 野719 (56)参考文献 特開 昭62−6670(JP,A) 特開 昭61−56898(JP,A) ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Masatsugu Ueda 1672-1 Imafuku, Kawagoe City, Saitama Prefecture Maison Musashino 719 (56) References JP 62-6670 (JP, A) JP 61-56898 ( JP, A)

Claims (1)

【特許請求の範囲】[Claims] 【請求項1】細胞培養槽の一部に、培養液は透過するが
細胞の接着担体は透過しないフィルターによって隔離さ
れていて上方は細胞培養槽内の気体層部分において解放
している領域を設けるとともに、該領域内に酸素及びま
たは空気を供給する導管を配設して培養液中に酸素を供
給するようにし、細胞は該領域外で培養するようにした
細胞培養槽であって、該フィルターを、回転あるいは振
動するようにしたことを特徴とする細胞培養槽。
1. A part of a cell culture tank is provided with a region which is isolated by a filter through which a culture solution permeates but does not permeate an adhesive carrier of cells and which is open above in a gas layer portion in the cell culture tank. At the same time, a conduit for supplying oxygen and / or air is arranged in the region to supply oxygen into the culture medium, and the cell is a cell culture tank for culturing outside the region, and the filter is used. A cell culture vessel characterized in that it is adapted to rotate or vibrate.
JP62336366A 1987-12-28 1987-12-28 Cell culture tank Expired - Lifetime JPH078229B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62336366A JPH078229B2 (en) 1987-12-28 1987-12-28 Cell culture tank

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62336366A JPH078229B2 (en) 1987-12-28 1987-12-28 Cell culture tank

Publications (2)

Publication Number Publication Date
JPH01174376A JPH01174376A (en) 1989-07-10
JPH078229B2 true JPH078229B2 (en) 1995-02-01

Family

ID=18298393

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62336366A Expired - Lifetime JPH078229B2 (en) 1987-12-28 1987-12-28 Cell culture tank

Country Status (1)

Country Link
JP (1) JPH078229B2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3406710A1 (en) 2015-01-26 2018-11-28 UBE Industries, Ltd. Method, device and kit for mass cultivation of cells using polyimide porous membrane

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH072000Y2 (en) * 1989-09-25 1995-01-25 オリンパス光学工業株式会社 Irrigation device
JP2984419B2 (en) * 1991-02-28 1999-11-29 雪印乳業株式会社 Cell culture tank
JP6423942B1 (en) * 2017-11-08 2018-11-14 佐竹化学機械工業株式会社 Microcarrier stirring culture device

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6156898A (en) * 1984-08-21 1986-03-22 レンゴ−株式会社 Puncher for sheet
JPH0644860B2 (en) * 1985-07-03 1994-06-15 日本合成ゴム株式会社 Incubator

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3406710A1 (en) 2015-01-26 2018-11-28 UBE Industries, Ltd. Method, device and kit for mass cultivation of cells using polyimide porous membrane
US10479974B2 (en) 2015-01-26 2019-11-19 Ube Industries, Ltd. Method, device and kit for mass cultivation of cells using polyimide porous membrane
US10696943B2 (en) 2015-01-26 2020-06-30 Ube Industries, Ltd. Method, device and kit for mass cultivation of cells using polyimide porous membrane

Also Published As

Publication number Publication date
JPH01174376A (en) 1989-07-10

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