JPH0767695A - Method for smearing specimen and device therefor - Google Patents
Method for smearing specimen and device thereforInfo
- Publication number
- JPH0767695A JPH0767695A JP5219548A JP21954893A JPH0767695A JP H0767695 A JPH0767695 A JP H0767695A JP 5219548 A JP5219548 A JP 5219548A JP 21954893 A JP21954893 A JP 21954893A JP H0767695 A JPH0767695 A JP H0767695A
- Authority
- JP
- Japan
- Prior art keywords
- petri dish
- smearing
- sample
- automatically
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000005070 sampling Methods 0.000 claims description 42
- 239000002609 medium Substances 0.000 claims description 35
- 239000001963 growth medium Substances 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 5
- 238000011109 contamination Methods 0.000 abstract description 4
- 239000000523 sample Substances 0.000 description 48
- 238000004080 punching Methods 0.000 description 12
- 210000000078 claw Anatomy 0.000 description 11
- 238000010586 diagram Methods 0.000 description 11
- 230000003028 elevating effect Effects 0.000 description 8
- 238000012790 confirmation Methods 0.000 description 4
- 238000007689 inspection Methods 0.000 description 3
- 229910001120 nichrome Inorganic materials 0.000 description 3
- 239000012488 sample solution Substances 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 238000005553 drilling Methods 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- ZZUFCTLCJUWOSV-UHFFFAOYSA-N furosemide Chemical compound C1=C(Cl)C(S(=O)(=O)N)=CC(C(O)=O)=C1NCC1=CC=CO1 ZZUFCTLCJUWOSV-UHFFFAOYSA-N 0.000 description 1
- 230000020169 heat generation Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000004973 liquid crystal related substance Substances 0.000 description 1
- 238000012009 microbiological test Methods 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/50—Means for positioning or orientating the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
- C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
【0001】[0001]
【産業上の利用分野】本発明は、寒天などの培地を入れ
たシャーレに対して、被検査対象物、すなわち検体を塗
抹する方法およびその装置に関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method and an apparatus for smearing an object to be inspected, that is, a sample on a petri dish containing a medium such as agar.
【0002】[0002]
【従来の技術】培地に対する検体の塗抹は手作業によっ
て行っている。特に多数の検体を検査する必要がある場
合には、各々一個のシャーレに対して個々の検体を各々
手作業で順次塗抹している。微生物検査においては、検
体の採取が適切でないと後の検査をいかに正確に行って
も正しい値はえられない。二次汚染を防ぎ正しい検体の
採取をしなければならない。一個のシャーレに対して複
数の検体を塗抹できるにしても、培養後に判定を行う段
階で、整然と区分されていないと正確な判定が困難にな
る。培地に対する検体の塗抹は、適正な区分をもった塗
抹パターンが必要であり、手作業でその適正な塗抹パタ
ーンを形成するためには熟練を要すると共に能率が上が
らない。そこで、特開平3ー175996号公報に手作
業によらない培地に対する検体の塗抹方法が提案されて
いる。2. Description of the Related Art A sample is smeared on a medium by hand. When it is necessary to inspect a large number of specimens, the individual specimens are sequentially smeared on each petri dish by hand. In the microbiological test, if the sample is not properly collected, the correct value cannot be obtained no matter how accurately the subsequent tests are performed. It is necessary to prevent secondary contamination and collect a correct sample. Even if a plurality of specimens can be smeared on one petri dish, accurate determination becomes difficult if they are not sorted in an orderly manner at the stage of determination after culture. The smearing of a sample on a medium requires a smearing pattern having appropriate divisions, and it requires skill and is inefficient to manually form the appropriate smearing pattern. Therefore, JP-A-3-175996 proposes a method of smearing a sample onto a medium that does not require manual work.
【0003】[0003]
【発明が解決しようとする課題】特開平3ー17599
6号公報で開示されているのは、手作業によらない培地
に対する検体の塗抹方法であるが、検体の塗抹におい
て、作業能率の向上と同時に多数の塗抹前シャーレのス
トックおよび複数の検体の適正な区分をもつた塗抹パタ
ーンの形成を解決しなければならない課題を有してい
る。したがって本発明は検体の採取が適切に二次汚染を
防ぎ正しい検体の採取を自動で行うために、多数の塗抹
前シャーレのストックおよび複数の検体の適正な区分を
もった塗抹パターンを形成させて、塗抹後の多数のシャ
ーレを自動でストックすることを目的とするものであ
る。[Problems to be Solved by the Invention] JP-A-3-17599
No. 6 discloses a method of smearing a sample on a medium that does not require manual work. However, in the smearing of a sample, the working efficiency is improved, and at the same time, the stock of a large number of petri dishes before smearing and the suitability of a plurality of samples are appropriate. There is a problem that it is necessary to solve the formation of a smear pattern having various divisions. Therefore, the present invention forms a smear pattern having a stock of a large number of pre-smear petri dishes and a proper division of a plurality of samples in order to automatically collect a correct sample in order to prevent secondary contamination by appropriately collecting a sample. The purpose is to automatically stock a large number of petri dishes after smearing.
【0004】[0004]
【課題を解決するための手段】本発明は以上のような目
的を達成するために次のような検体の塗抹方法およびそ
の装置を提供するものである。すなわち、製品上を自動
で加熱滅菌された穿孔棒で穿孔した後、自動で加熱滅菌
された採取具で検体を採取して、培地の入ったシャーレ
に検体を自動で連続して塗抹する方法でありこの方法を
実施する装置としては、製品を搬送するコンベア手段
と、製品上を穿孔する加熱ヒーター付き穿孔棒手段と、
穿孔箇所から検体を採取する加熱ヒーター付き採取具手
段と、採取具によって検体を採取し、培地の入ったシャ
ーレに塗抹する手段と、該塗抹後のシャーレおよび塗抹
前のシャーレを搬送する手段を備えたことを特徴とする
自動連続塗抹装置である。In order to achieve the above-mentioned objects, the present invention provides the following specimen smearing method and apparatus. That is, after perforating the product with a perforated rod that is automatically heat-sterilized, a sample is collected with a sampling tool that is automatically heat-sterilized, and the sample is automatically and continuously smeared on a petri dish containing the medium. There is a device for carrying out this method, a conveyor means for conveying the product, and a piercing rod means with a heating heater for piercing the product,
Equipped with a collecting tool means with a heating heater for collecting a sample from the perforated portion, a means for collecting a sample by the collecting tool, smearing it on a petri dish containing medium, and a means for conveying the petri dish after the smearing and the petri dish before smearing. This is an automatic continuous smearing device.
【0005】[0005]
【作用】本発明は、培地側と検体塗抹手段とを相対的に
所定の方向に移動させて、一定方向に検体を塗抹するこ
とを特徴とする、培地に対する検体の塗抹方法を提供す
るものであり、培地の入った一個のシャーレに対して複
数の検体を塗抹する手段を有し、これらを相対的に所定
の方向に移動させて、複数の検体を各々一定の方向に塗
抹するものであり、一つのシャーレに複数の検体を塗抹
しても整然と区分された状態になっているので、その後
の検査工程において誤りが生じない。The present invention provides a method for smearing a sample on a medium, characterized in that the medium side and the sample smearing means are relatively moved in a predetermined direction to smear the sample in a fixed direction. Yes, it has a means for smearing a plurality of specimens on one dish containing medium, and moving these in a predetermined direction relative to each other, a plurality of specimens are smeared in a certain direction, respectively. Even if a plurality of specimens are smeared on one petri dish, they are in an orderly divided state, so that no error occurs in the subsequent inspection process.
【0006】[0006]
【実施例】以下図面に示す実施例について説明する。本
発明の方法は、陽圧の無菌エアーで囲まれた部分で塗抹
を行うものであって、製品上を自動で加熱滅菌された穿
孔棒で穿孔した後、自動で加熱滅菌された採取具で検体
を採取して、培地の入ったシャーレに検体を自動で連続
して塗抹することを特徴とする検体の塗抹方法である
が、これを実施する装置をかりて説明する。本発明の自
動塗抹装置は、次の構成からなっている。 A トレイ搬送部 B 穿孔部 C サンプリング、塗抹部 D シャーレ搬送部 E クリーンフアン部 本発明の自動塗抹装置に使用される一つのトレイは、製
品(例えばポーションクリーム又は250ml入り牛
乳)をそれぞれ規則正しく並べる。そして、トレイ6個
とシャーレ20個をセットできるようにする。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The embodiments shown in the drawings will be described below. The method of the present invention is to smear in a portion surrounded by positive-pressure aseptic air, and after piercing the product with a piercing rod that is automatically heat-sterilized, use a collecting tool that is automatically heat-sterilized. A sample smearing method is characterized by collecting a sample and automatically and continuously smearing the sample onto a petri dish containing a medium. The method for smearing the sample will be described with reference to an apparatus for carrying out the method. The automatic smearing device of the present invention has the following configuration. A tray transporting section B perforating section C sampling, smearing section D petri dish transporting section E clean fan section In one tray used in the automatic smearing apparatus of the present invention, products (for example, potion cream or milk containing 250 ml) are regularly arranged. Then, 6 trays and 20 petri dishes can be set.
【0007】(1)トレイ搬送部 そこで、トレイ搬送部Aから説明すると、製品(サンプ
ル)入りトレイ1を図4において、右側57の下方から
上方に搬送するチェンコンベア59が図2に示す如く装
置され、そのチェンコンベア59の上端において、図示
しない縦送りコンベアが図4の右側57から左側58に
トレイ1を搬送するように装置されており、更に左側5
8において、図4の点線で示す如く検体の終わったトレ
イ1を上方から下方に搬送する図示しないチェンコンベ
アがある。以上のチェンコンベア59や縦送りコンベア
は間欠移動するが、とくに縦送りコンベアは後述する穿
孔部、サンプリング塗抹部を経由するので、その作業中
は停止する。以上の如く縦送りコンベアは、製品入りト
レイを穿孔部、サンプリング塗抹部を経由せしめてそれ
ぞれの位置において製品は穿孔、サンプリング、塗抹作
業をうけることになる。その際、穿孔部の手前では、ト
レイ中の製品識別及び製品の有無を図4に示す光電セン
サー5で検出する。この検出作業後トレイは穿孔部Bに
至る。次に穿孔部Bについて図5、図6、図7に基づい
て説明する。(1) Tray Conveying Section Therefore, starting from the tray conveying section A, a chain conveyor 59 for conveying the product (sample) -containing tray 1 from below right side 57 to above in FIG. 4 is a device as shown in FIG. At the upper end of the chain conveyor 59, a vertical feed conveyor (not shown) is installed to convey the tray 1 from the right side 57 to the left side 58 in FIG.
In FIG. 8, there is a chain conveyor (not shown) for transporting the tray 1 on which the sample is finished from the upper side to the lower side as shown by the dotted line in FIG. The chain conveyor 59 and the vertical feed conveyor described above move intermittently, but since the vertical feed conveyor passes through a punching portion and a sampling smear portion, which will be described later, it is stopped during the work. As described above, in the vertical feed conveyor, the tray containing the product is passed through the punching portion and the sampling smearing portion, and the product is punched, sampled and smeared at each position. At that time, in front of the punching portion, the photoelectric sensor 5 shown in FIG. 4 detects the product identification in the tray and the presence or absence of the product. After this detection work, the tray reaches the punching portion B. Next, the punched portion B will be described with reference to FIGS. 5, 6 and 7.
【0008】(2) 穿孔部B 穿孔部Bは、モーター6のプーリー7からベルトを介し
て駆動されるプーリー8の螺軸9で上下動する昇降枠1
0があり、この昇降枠10の横バー11に穿孔棒3が5
個取り付けられている。昇降枠10は、固定枠13のガ
イド14に沿って昇降するもので、穿孔棒3を加熱する
ためのヒーター12が穿孔棒3と共に横バー11に設け
られており、トレイ中の製品を識別するための光電セン
サー16が固定枠13の固定バー15に設けられてい
る。以上のような穿孔部は、トレイ中の製品(サンプ
ル)に後述する採取具を挿入するための穴Sを開けるも
のであり、前述した如く穿孔棒3は、通電したヒーター
12で焼き開けるもので、各サンプルに一つづつ穿孔す
る。穿孔用ヒーターは300℃に加熱される。穿孔後に
穿孔棒3はモーター6、螺軸9、昇降枠10で上昇し、
トレイは縦送りコンベアにより、図4で左方向に移動
し、次の製品の穿孔を行う。以上のように穿孔されたト
レイは縦送りコンベアにより、次のサンプリング、塗抹
部Cに移動する。(2) Punching part B The punching part B is a lifting frame 1 which moves up and down by a screw shaft 9 of a pulley 8 driven by a pulley 7 of a motor 6 via a belt.
0, and the horizontal bar 11 of the lifting frame 10 has 5
Individually installed. The elevating frame 10 ascends and descends along the guide 14 of the fixed frame 13, and a heater 12 for heating the piercing bar 3 is provided on the horizontal bar 11 together with the piercing bar 3 to identify the product in the tray. A photoelectric sensor 16 is provided on the fixed bar 15 of the fixed frame 13. The perforation section as described above is for making a hole S for inserting a sampling tool described later in the product (sample) in the tray, and as described above, the perforation rod 3 is made by baking with the heater 12 which is energized. , Perforate one for each sample. The perforation heater is heated to 300 ° C. After drilling, the drilling rod 3 is raised by the motor 6, the screw shaft 9, and the elevating frame 10,
The tray is moved leftward in FIG. 4 by the vertical feed conveyor to punch the next product. The tray perforated as described above is moved to the next sampling and smearing section C by the vertical feed conveyor.
【0009】(3) サンプリング、塗抹部C サンプリング、塗抹部Cは、図8、図9、図10に示さ
れており、図示しないモーターで回転する螺軸19で昇
降する昇降ブロック17の横ロッド18に採取具4が5
個取り付けられており、螺軸19と平行に昇降用ガイド
ロッド19aがあり、昇降ブロック17はこれにガイド
されて昇降する。ガイドロッド19aに対向してガイド
ロッド20があり、これに沿って昇降する昇降ブロック
21が昇降ブロック17に対向して設けられ、横ロッド
18はこれら昇降ブロック17、21間に横架されてい
る。螺軸19とガイドロッド19aとは、横移動ブロッ
ク22に植立され、ガイドロッド20は横移動ブロック
23に植立されている。そして、横移動ブロック22、
23はガイドロッド25、26に沿って移動し、横移動
ブロック22は螺軸24で図示しないモーターで横移動
する。昇降ブロック17、21の上方には又ブロック2
7、28があって、ガイドロッド29、30に沿って横
移動するようになっている。採取具4は、ニクロム線を
使用したもので電気を流し電熱滅菌できるようになって
いる。(4a参照)採取具4は、螺軸19によって下降
し、製品(サンプル)中に前述した穿孔Sを通じて挿入
される。そして、採取具の先に付着した検体(製品サン
プル)を培地に塗抹すべく螺軸19によって再び所定の
位置まで上昇させるようになっている。上昇したら図示
しないモーターにより、螺軸24を介して採取具4を横
移動させる。そのとき、横移動ブロック22、23、2
7、28は、ロッド25、26、29、30に沿って移
動する。そして横移動後培地に塗抹する検体をもつた採
取具を培地に向かって下降せしめるようになっている。
更に培地に塗抹すると採取具4は、所定の位置に戻り採
取具のニクロム線の部分を加熱滅菌する。次に真下に製
品が搬送されると、ヒーターは切れて製品中に挿入され
るようになっている。(3) Sampling and smearing section C Sampling and smearing section C are shown in FIGS. 8, 9 and 10, and the horizontal rod of the elevating block 17 is moved up and down by a screw shaft 19 rotated by a motor (not shown). 18 has a sampling tool 4 of 5
Individually attached, there is an elevating guide rod 19a parallel to the screw shaft 19, and the elevating block 17 is guided by this and ascends and descends. There is a guide rod 20 facing the guide rod 19a, and an elevating block 21 that moves up and down along the guide rod 20 is provided so as to oppose the elevating block 17, and a horizontal rod 18 is laid horizontally between these elevating blocks 17 and 21. . The screw shaft 19 and the guide rod 19 a are erected in the lateral movement block 22, and the guide rod 20 is erected in the lateral movement block 23. Then, the lateral movement block 22,
23 moves along the guide rods 25 and 26, and the lateral movement block 22 laterally moves by a screw shaft 24 by a motor (not shown). Above the lifting blocks 17 and 21, the block 2 is also provided.
7 and 28, which are adapted to move laterally along the guide rods 29 and 30. The sampling tool 4 is made of a nichrome wire and can be sterilized by applying heat by passing electricity. (Refer to 4a) The sampling tool 4 is lowered by the screw shaft 19 and inserted into the product (sample) through the above-mentioned perforation S. Then, in order to smear the sample (product sample) attached to the tip of the collecting tool on the medium, the screw shaft 19 is used to raise the sample again to a predetermined position. When it rises, the sampling tool 4 is laterally moved via the screw shaft 24 by a motor (not shown). At that time, the lateral movement blocks 22, 23, 2
7, 28 move along rods 25, 26, 29, 30. Then, after the lateral movement, the sampling tool having the specimen to be smeared on the medium is lowered toward the medium.
When the medium is further smeared, the sampling tool 4 returns to a predetermined position and the nichrome wire portion of the sampling tool is heat-sterilized. Then, when the product is conveyed right below, the heater is cut off and inserted into the product.
【0010】そこで、塗抹について説明する。塗抹位置
には、台枠31にモーター32が設けられ、モーター3
2のギヤから駆動されるギヤ36の軸33によつてこれ
に固定された支持ブラケット34、34を介して傾動せ
しめられるシャーレ載置台35がある。ブラケット3
4、34間のブラケット38は軸33に嵌装され、螺軸
37に螺合して螺軸37の図示しないモーターからベル
トを介して駆動されることによりシャーレ載置台35は
トレーの進行方向に直交する方向に横移動する。以上の
ようにして塗抹位置には、シャーレ載置台35があるの
で、採取具4がシャーレ載置台の位置にくると、シャー
レ載置台35は、モーター32により、傾斜し、この傾
斜した状態でシャーレ2は採取具に対応する。採取具4
は横移動しつづけてシャーレ2に塗抹する。その際、傾
いて待ち構えている培地の入ったシャーレ2に採取具4
の先が培地に接しながら、シャーレ2の傾きは水平位置
になり、図4に示す光電センサー39によって塗抹した
か否かを確認する。そして、採取具4が進行方向に移動
して培地に検体の線ができる。次に培地の入ったシャー
レ2は進行方向に対して直交方向に7mmずつ横移動し
て採取具4は採取する位置に戻り培地の入ったシャーレ
2も傾き状態になり次の塗抹をまつ。採取具の数は5つ
であって一つのシャーレに30(250ml1トレイ
分)塗抹する。例えば250mlの場合の図11はその
状態を示すもので図12に示す製品の穿孔Sした位置か
ら製品を採取して塗抹するようになっている。図12の
製品イ列、ロ列、ハ列、ニ列、ホ列に対応して図11に
検体を塗抹した線が示されている。以上のような採取具
の移動工程を図で示すと図13のようになる。図中の
a、b,c、d、e、f、g、h は下記の状態を表
す。 a 培地位置まで左へ移動(塗抹スタート位置) b 培地位置で下降(塗抹スタート位置) c 採取具が培地面を左へ移動 d 塗抹完了で上昇 e 塗抹完了で採取具は発熱用端子位置まで右へ移動 f 発熱完了した採取具をサンプル液取り出し位置まで
左へ移動 g サンプル液取り出しのため下降 h サンプル液を取り出して上昇 次にシャーレ搬送部について説明する。The smear will be described. At the smearing position, the motor 32 is provided on the underframe 31 and the motor 3
There is a petri dish mounting base 35 which is tilted by a shaft 33 of a gear 36 driven from the second gear via support brackets 34, 34 fixed to the shaft 33. Bracket 3
The bracket 38 between 4 and 34 is fitted on the shaft 33, and is screwed onto the screw shaft 37 and driven by a motor (not shown) of the screw shaft 37 via a belt, whereby the petri dish mounting table 35 is moved in the tray advancing direction. Move laterally in the orthogonal direction. As described above, since the petri dish mounting table 35 is located at the smearing position, when the sampling tool 4 reaches the petri dish mounting table position, the petri dish mounting table 35 is tilted by the motor 32, and the petri dish is tilted in this tilted state. 2 corresponds to a sampling tool. Collection tool 4
Continue to move laterally and smear on the petri dish 2. At that time, the collection tool 4 is placed on the Petri dish 2 containing the culture medium that is tilted and waiting.
While the tip of the plate is in contact with the medium, the inclination of the dish 2 becomes horizontal, and it is confirmed by the photoelectric sensor 39 shown in FIG. Then, the sampling tool 4 moves in the traveling direction to form a sample line on the medium. Next, the petri dish 2 containing the medium is laterally moved by 7 mm in a direction orthogonal to the traveling direction, the sampling tool 4 returns to the sampling position, and the petri dish 2 containing the culture medium is also tilted to coat the next smear. The number of collecting tools is 5, and 30 (250 ml, 1 tray) is smeared on one petri dish. For example, in the case of 250 ml, FIG. 11 shows that state, and the product is sampled from the position where the product has been perforated S shown in FIG. 12 and smeared. Lines smeared with the sample are shown in FIG. 11 corresponding to the product A row, the row B, the row C, the row D, and the row E in FIG. FIG. 13 shows the steps of moving the sampling tool as described above. In the figure, a, b, c, d, e, f, g and h represent the following states. a Move to the left side to the culture medium position (smear start position) b Lower at the medium position (smear start position) c Sampling tool moves to the left side of the culture medium d Raises when smearing is completed e Sampling tool is right to the heating terminal position when smearing is completed Move to f f Move the sampling tool that has completed heat generation to the left to the sample solution removal position g Descend for sample solution removal h Take out sample solution and raise it Next, the petri dish transfer unit will be described.
【0011】(4) シャーレ搬送部D シャーレ搬送部Dはシャーレ2を前記したシャーレ載置
台35に供給し、塗抹し終わったシャーレ2をストック
位置に搬送するためのものであるが塗抹部との関連でこ
れを説明する。図14は図4の一部を拡大した図面を示
すもので40は塗抹前のシャーレストック位置を示し2
0個のシャーレをストックできるようにする。又41は
塗抹後のシャーレ2のストック位置を示す。更に42は
シャーレ2の蓋43をおく位置を示している。そしてシ
ャーレ搬送部は次のようなものからなっている。すなわ
ち、39は塗抹確認センサーであり、44はシャーレ横
搬送ベルトである更に60はシャーレ分離用爪、61は
シャーレストック用爪、62はシャーレ上下搬送用ホル
ダー、63はシャーレ搬送用リフト、64は送りネジ、
65はシャーレ上下搬送用ホルダーである。次にシャー
レの流れの順序について説明すると、次のようである。 イ 図15の如くシャーレ上下搬送用ホルダー62が上
がりシャーレ2がシャーレ上下搬送用ホルダー62に載
った時点でシャーレ分離用爪60が開く。 ロ 図16に示す如くシャーレ上下搬送用ホルダー62
が一定位置まで下がりシャーレ分離用爪60が閉じるこ
とによってシャーレストック位置40における上方のシ
ャーレ2から下方のシャーレ2を分離する。 ハ 図17に示す如くシャーレ上下搬送ホルダー62は
更に下がり分離したシャーレ2をシャーレ横搬送ベルト
44上に移す。 ニ 図18に示す如くシャーレ搬送リフト63の爪が上
がりシャーレ2をベルト44からすくい送ネジ64によ
って前進する。 ホ 図19に示す如くシャーレ蓋置き42の位置でシャ
ーレ搬送リフト63の爪を下げシャーレ2の蓋43をシ
ャーレ蓋置き42に置く。 ヘ 図20に示す如く、シャーレ搬送リフト63は更に
前進し、シャーレ載置台35まで進み、その爪を下げ、
シャーレ2をシャーレ載置台35に移し、シャーレ搬送
リフト63は後退する。 ト 図21はシャーレ2がシャーレ載置台35にセット
された状態を示す。 チ 採取具4の進入に合わせてシャーレ載置台35を図
22のようにモータ32によって動かし培地への塗抹時
採取具4と培地面との角度θを小さくし、培地への喰い
込みをなくしθを大きくすることによって塗抹する。シ
ャーレ載置台35が水平に戻った時点で塗抹確認センサ
ー39が進入し、シャーレ載置台35を7mm横移動さ
せ、センサリングすると同時に次の塗抹に備える。塗抹
確認センサー39の作動を確実ならしめるため図14の
b視図に示す如くシャーレ載置台35に切欠き35aが
ある。 リ 図23に示す如くシャーレ搬送用リフト63が進入
し、シャーレ載置台35からシャーレ2をすくう。 ヌ 図24に示す如くシャーレ蓋置き42の位置まで戻
った時点でシャーレ搬送リフト63の爪を上げ蓋43を
かぶせる。 ル シャーレ搬送用リフト63は更に戻り、シャーレ横
搬送用ベルト44上に図25の如くシャーレ2を移す。 ヲ 図26の如くシャーレ横搬送ベルト44を動か
し、、シャーレ2を塗抹後のシャーレストック位置41
の位置に移動させる。 ワ 図27の如くシャーレ上下搬送用ホルダー65が上
がる。 カ 図28の如くシャーレストック用爪61が開き、シ
ャーレストック用爪61上のシャーレ2がシャーレ上下
搬送用ホルダー65に移る。 ヨ 図29の如くシャーレ上下搬送用ホルダー65がさ
らに上がりシャーレストック用爪61が閉ざしシャーレ
2がシャーレストック用爪61に移る。 タ シャーレ上下搬送用ホルダー65が図30の如く下
がる。 以上のような工程を経てシャーレは、シャーレストック
位置40から途中で蓋を外してシャーレ載置台35の位
置まで送られ、塗抹し終わると途中で蓋をかぶせてスト
ック位置41まで搬送されるようになっている。次にク
リーンフアン部Eについて説明する。(4) Petri dish transporting section D The petri dish transporting section D is for feeding the petri dish 2 to the above-mentioned petri dish mounting table 35 and for transporting the petri dish 2 which has been smeared to the stock position. This will be explained in connection. FIG. 14 shows an enlarged view of a part of FIG. 4, and 40 shows the petri dish stock position before smearing.
Make it possible to stock 0 petri dishes. Reference numeral 41 indicates the stock position of the petri dish 2 after smearing. Reference numeral 42 indicates a position where the lid 43 of the petri dish 2 is placed. The petri dish transport section is composed of the following. That is, 39 is a smearing confirmation sensor, 44 is a petri dish lateral transport belt, 60 is a petri dish separating claw, 61 is a petri dish stock claw, 62 is a petri dish vertical transport holder, 63 is a petri dish transport lift, and 64 is a petri dish transport lift. Lead screw,
Reference numeral 65 is a holder for vertically transporting the petri dish. Next, the flow order of the petri dish will be described as follows. B. As shown in FIG. 15, the petri dish upper and lower transporting holders 62 are raised, and when the petri dish 2 is placed on the petri dish upper and lower transporting holders 62, the petri dish separating claws 60 are opened. (B) As shown in FIG. 16, the petri dish upper and lower transport holders 62
Is lowered to a certain position and the petri dish separating claw 60 is closed to separate the lower petri dish 2 from the upper petri dish 2 at the petri dish stock position 40. As shown in FIG. 17, the petri dish upper and lower transport holders 62 are further lowered to transfer the separated petri dish 2 onto the horizontal petri dish transport belt 44. D As shown in FIG. 18, the claws of the petri dish transport lift 63 are raised and the petri dish 2 is advanced from the belt 44 by the scooping screw 64. As shown in FIG. 19, the pawl of the petri dish transport lift 63 is lowered at the position of the petri dish lid rest 42 and the lid 43 of the petri dish 2 is placed on the petri dish lid rest 42. F As shown in FIG. 20, the petri dish transfer lift 63 further advances to the petri dish mounting table 35, and lowers its claws.
The petri dish 2 is moved to the petri dish mounting table 35, and the petri dish transport lift 63 is retracted. 21. FIG. 21 shows a state in which the Petri dish 2 is set on the Petri dish mounting table 35. H. The petri dish mounting table 35 is moved by the motor 32 as shown in FIG. 22 in accordance with the entry of the sampling tool 4, and the angle θ between the sampling tool 4 and the culture medium surface at the time of smearing on the medium is made small so as not to bite into the medium θ. Smear by increasing. When the petri dish mounting table 35 returns to the horizontal position, the smearing confirmation sensor 39 enters and the petri dish mounting table 35 is laterally moved by 7 mm to perform sensoring and prepare for the next smearing. In order to ensure the operation of the smearing confirmation sensor 39, the petri dish mounting table 35 has a notch 35a as shown in FIG. 23. As shown in FIG. 23, the petri dish transfer lift 63 enters and scoops the petri dish 2 from the petri dish mounting table 35. 24. As shown in FIG. 24, when returning to the position of the petri dish lid holder 42, the claws of the petri dish transport lift 63 are lifted and the lid 43 is covered. The petri dish transport lift 63 is further returned, and the petri dish 2 is transferred onto the petri dish lateral transport belt 44 as shown in FIG. 26. As shown in FIG. 26, move the petri dish lateral transfer belt 44 to apply the petri dish 2 to the petri dish stock position 41 after smearing.
Move to the position. W As shown in FIG. 27, the petri dish upper and lower transporting holders 65 are raised. 28. As shown in FIG. 28, the petri dish stock claw 61 is opened, and the petri dish 2 on the petri dish stock claw 61 is transferred to the petri dish vertical transport holder 65. 29. As shown in FIG. 29, the petri dish vertical transport holder 65 further rises, the petri dish stock claw 61 is closed, and the petri dish 2 moves to the petri dish stock claw 61. The vertical transport holder 65 for the dish is lowered as shown in FIG. Through the above steps, the petri dish is sent from the petri dish stock position 40 to the petri dish mounting table 35 with the lid removed midway, and after the smearing is completed, the petri dish is covered with the lid and conveyed to the stock position 41. Has become. Next, the clean fan part E will be described.
【0012】(5) クリーンファン部E クリーンファン部Eは図4において55で示す。前述し
た塗抹は陽圧の無菌エアーで囲まれたクリーンブース内
で行う。図1、図2に示す45は無菌室を構成する遮断
カーテンを示す。ここで穿孔、塗抹する数は1サイクル
5個とし、250mlの場合1トレイ(サンプル30
個)を6サイクルで処理する塗抹能力とする。6トレイ
(サンプル180個)の処理に要する時間は25分かか
るが、穿孔部、採取具の手段の時間を短縮することで時
間は短縮される。又塗抹確認機構としては、各トレイの
1サイクル目の塗抹跡(培地面)は、前述した塗抹セン
サーとしての光電センサー39で確認する。異常の時
は、ブザー音を発し機械を停止させる。(5) Clean Fan Section E The clean fan section E is designated by 55 in FIG. The above-mentioned smearing is performed in a clean booth surrounded by aseptic air of positive pressure. Reference numeral 45 shown in FIGS. 1 and 2 denotes a shut-off curtain which constitutes a sterile room. Here, the number of holes to be perforated and smeared is 5 per cycle. For 250 ml, 1 tray (sample 30
Each piece) has a smearing ability of 6 cycles. It takes 25 minutes to process 6 trays (180 samples), but the time can be shortened by shortening the time of the means of the perforation part and the collecting tool. As a smear confirmation mechanism, the first cycle smear trace (medium surface) of each tray is confirmed by the photoelectric sensor 39 as the smear sensor described above. In case of abnormality, a buzzer sounds and the machine is stopped.
【0013】図1は、本発明装置の正面図、図2は側面
図、図3は平面図をそれぞれ示すもので以上説明した装
置で構成されており、筺体全体は移動用キャスター46
で移動自在であり、設置用アジャスター47で所定位置
に筺体を固定できるようになつている。図中48は電装
部開閉用扉、49は始動スイッチ、50は液晶ディスプ
レイでタッチスイッチを内蔵し、操作内容を表示するよ
うになっている。51はパトライトで動作状態をライト
でしらせるものである。52は緊急停止スイッチであっ
て図1における53は製品識別エリア、54は塗抹エリ
アをそれぞれ示す。FIG. 1 is a front view of the device of the present invention, FIG. 2 is a side view thereof, and FIG. 3 is a plan view of the device, which are constituted by the devices described above.
The housing is fixed by the adjuster 47 for installation at a predetermined position. In the figure, reference numeral 48 is a door for opening / closing the electrical equipment, 49 is a start switch, and 50 is a liquid crystal display with a built-in touch switch for displaying operation contents. Reference numeral 51 is a patrol light for indicating the operating state by a light. Reference numeral 52 is an emergency stop switch, 53 in FIG. 1 is a product identification area, and 54 is a smearing area.
【0014】前述した装置の作動を総括して述べると、
トレイ1は図2に示すチエンコンベア59によって1つ
のトレイ1の検体が終わると、次のトレイ1が上にあが
るようになっており、検体が終わったトレイ1は図示し
ない縦送りコンベアによって図4の57の位置から58
の位置のトレイ収納部へ送られ上から下に自動的にチェ
ンコンベアで移動するようになる。そして、チェンコン
ベア59によつて上に上がったトレイ1は縦送りコンベ
アによって送られ、穿孔部Bの手前でトレイ中の製品識
別および製品の有無を光電センサー5で検出する。次に
製品サンプルに採取具4を挿入するための穴Sをあける
のに穿孔棒3は電気ゴテ式を用いた電気抵抗を利用して
焼き開ける。穿孔棒は、5つ並んで各サンプル1つずつ
穿孔する穿孔後に穿孔棒3は上昇し、トレイ1は横送り
コンベアにより移動し、次の穿孔を行う。次の検体の採
取では、採取具4を製品(サンプル)に挿入し、採取具
4の先に付着した検体(製品サンプル)を培地に塗抹す
る。塗抹後所定位置に戻り、採取具4のニクロム線の部
分を加熱滅菌する。次に真下に製品(サンプル)が搬送
されてくると、加熱ヒーター4aは切れて製品(サンプ
ル)に挿入し、採取具4の先に検体を付着させると同時
に培地の入ったシャーレが進行方向と直交方向に移動
し、塗抹を行う。この繰り返し動作を行って塗抹する。The operation of the above-mentioned apparatus will be summarized as follows.
When the sample of one tray 1 of the tray 1 is finished by the chain conveyor 59 shown in FIG. 2, the next tray 1 is raised, and the tray 1 after the sample is finished by the vertical feed conveyor (not shown). From position 57 of 58
It will be sent to the tray storage part at the position of and will be automatically moved from top to bottom by the chain conveyor. Then, the tray 1 raised by the chain conveyor 59 is fed by the vertical feed conveyor, and the photoelectric sensor 5 detects the product identification in the tray and the presence or absence of the product in front of the punching portion B. Next, in order to make a hole S into which the sampling tool 4 is inserted in the product sample, the piercing rod 3 is baked by utilizing the electric resistance using the electric iron type. After piercing five piercing rods side by side for each sample, the piercing rod 3 moves up, and the tray 1 is moved by the transverse feed conveyor to perform the next piercing. In the next sample collection, the sampling tool 4 is inserted into the product (sample), and the sample (product sample) attached to the tip of the sampling tool 4 is smeared on the medium. After smearing, the nichrome wire portion of the sampling tool 4 is heat-sterilized by returning to a predetermined position. Next, when the product (sample) is conveyed directly below, the heater 4a is cut off and inserted into the product (sample), the specimen is attached to the tip of the sampling tool 4, and at the same time, the petri dish containing the medium moves in the traveling direction. Move in the orthogonal direction and smear. Repeat this operation to smear.
【0015】塗抹する時のシャーレの動作について説明
すると、採取具の先が培地に均一に塗抹できるようにモ
ーター32により傾いて待ち構えている培地の入ったシ
ャーレに採取具4の先が培地に接しながらシャーレの傾
きは水平位置になり、光電センサー39によって塗抹し
たか否かを確認する。採取具4が進行方向に移動して培
地に塗抹の線からなる塗抹跡ができる。ここで傾いたシ
ャーレ2に塗抹具4が接するのは塗抹しやすいからであ
る。培地に検体の線ができると、次に培地の入ったシャ
ーレ2は、進行方向に対して横方向に7mmずつ移動し
て採取具4は採取する位置に戻り、培地の入ったシャー
レ2も傾き状態になり、次の塗抹を待つ。以上の如くに
して塗抹し終わると、光電センサー39で確認され、シ
ャーレ2は図4の41位置へ搬送されてストックされ
る。したがって、本発明は1つの培地の入ったシャーレ
に数多くの異なった製品を採取して塗抹することができ
る。従来、人が何本も検体を一直線状に塗抹する場合、
接触する、または重なるなどの問題があったが、本発明
によれば、一直線状のものを何本も塗抹することができ
る。したがって、塗抹する位置の精度が高くなった。更
に1つのシャーレの培地の量に対して塗抹する検体の量
を多く塗ることができ、塗抹する検体の量を少なくして
数多くの検体を従来よりも多く塗抹することができる。
何れにしても本発明のものは、複数の検体を各々一定方
向に塗抹できるものであり、1つのシャーレに複数の検
体を塗抹しても整然と区分された状態になるのでその後
の検査工程において誤りが生じない。そこで多数の塗抹
前シャーレのストックおよび複数の適正な区分をもった
塗抹パターンを形成させて塗抹後の多数のシャーレを自
動でストックすることができる。The operation of the petri dish at the time of smearing will be described. The tip of the sampling tool 4 contacts the medium on the petri dish containing the medium that is tilted by the motor 32 so that the tip of the sampling tool can be uniformly smeared on the medium. Meanwhile, the inclination of the petri dish is at the horizontal position, and it is confirmed by the photoelectric sensor 39 whether or not the smear is applied. The sampling tool 4 moves in the traveling direction to form a smear trace consisting of a smear line on the medium. The smearing tool 4 comes into contact with the inclined petri dish 2 here because it is easy to smear. When the lines of the sample are formed in the medium, the petri dish 2 containing the medium then moves 7 mm laterally with respect to the traveling direction and the sampling tool 4 returns to the position for collecting, and the petri dish 2 containing the medium also tilts. Wait until the next smear. When the smearing is completed as described above, it is confirmed by the photoelectric sensor 39, and the petri dish 2 is transported to the position 41 in FIG. 4 and stocked. Therefore, according to the present invention, many different products can be collected and smeared on a petri dish containing one medium. Conventionally, when a person smears several specimens in a straight line,
Although there is a problem of contact or overlapping, according to the present invention, it is possible to smear a number of straight lines. Therefore, the accuracy of the smearing position is improved. Further, a large amount of the sample to be smeared can be applied with respect to the amount of the medium of one petri dish, and the amount of the sample to be smeared can be reduced so that a large number of the samples can be smeared more than before.
In any case, according to the present invention, it is possible to smear a plurality of specimens in a certain direction, and even if a plurality of specimens are smeared on one petri dish, the specimens will be in an orderly divided state, so that an error will occur in the subsequent inspection process. Does not occur. Therefore, a stock of a large number of petri dishes before smearing and a smearing pattern having a plurality of appropriate divisions can be formed to automatically stock a large number of petri dishes after smearing.
【0016】[0016]
【発明の効果】本発明は、検体の採取が適切に二次汚染
を防いだ正しい検体の採取を自動で行うことができる。
更に多数の塗抹前のシャーレのストックおよび複数の検
体の適正な区分をもつた塗抹パターンを形成させて、塗
抹後の多数のシャーレを自動でストックすることができ
るので、検査作業の省力化に向けた作業性の向上が図れ
る。EFFECTS OF THE INVENTION According to the present invention, it is possible to automatically collect a correct sample while appropriately collecting the sample and preventing secondary contamination.
Furthermore, it is possible to automatically stock a large number of petri dishes after smearing by forming a large number of petri dishes before smearing and a smearing pattern with proper division of multiple specimens, so it is possible to save labor in inspection work. The workability can be improved.
【図1】本発明装置の正面図。FIG. 1 is a front view of the device of the present invention.
【図2】本発明装置の側面図。FIG. 2 is a side view of the device of the present invention.
【図3】本発明の平面図。FIG. 3 is a plan view of the present invention.
【図4】本発明装置全体の説明図。FIG. 4 is an explanatory view of the entire device of the present invention.
【図5】穿孔部の正面図。FIG. 5 is a front view of a punching portion.
【図6】穿孔部の平面図。FIG. 6 is a plan view of a punching portion.
【図7】穿孔部の側面図。FIG. 7 is a side view of a punching portion.
【図8】サンプリング塗抹部の正面図。FIG. 8 is a front view of a sampling smear portion.
【図9】サンプリング塗抹部の側面図。FIG. 9 is a side view of a sampling smear section.
【図10】サンプリング塗抹部の平面図。FIG. 10 is a plan view of a sampling smear portion.
【図11】シャーレの培地に検体を塗抹した状態を示す
平面図。FIG. 11 is a plan view showing a state in which a sample is smeared on the medium of a petri dish.
【図12】トレイに製品を配列して穿孔した状態を示す
部分平面図。FIG. 12 is a partial plan view showing a state in which products are arranged on a tray and punched.
【図13】採取具の移動工程図。FIG. 13 is a movement process diagram of the sampling tool.
【図14】シャーレ搬送装置の構成図。FIG. 14 is a configuration diagram of a petri dish transport device.
【図15】シャーレ搬送作動説明図。FIG. 15 is an explanatory diagram of a petri dish transport operation.
【図16】シャーレ搬送作動説明図。FIG. 16 is an explanatory diagram of a petri dish transport operation.
【図17】シャーレ搬送作動説明図。FIG. 17 is an explanatory view of petri dish transport operation.
【図18】シャーレ搬送作動説明図。FIG. 18 is an explanatory view of a petri dish transport operation.
【図19】シャーレ搬送作動説明図。FIG. 19 is a diagram illustrating a petri dish transport operation.
【図20】シャーレ搬送作動説明図。FIG. 20 is an explanatory diagram of a petri dish transport operation.
【図21】シャーレ搬送作動説明図。FIG. 21 is an explanatory diagram of a petri dish transport operation.
【図22】シャーレ搬送作動説明図。FIG. 22 is an explanatory view of a petri dish transport operation.
【図23】シャーレ搬送作動説明図。FIG. 23 is an explanatory diagram of a petri dish transport operation.
【図24】シャーレ搬送作動説明図。FIG. 24 is an explanatory diagram of a petri dish transport operation.
【図25】シャーレ搬送作動説明図。FIG. 25 is an explanatory view of a petri dish transport operation.
【図26】シャーレ搬送作動説明図。FIG. 26 is an explanatory view of a petri dish transport operation.
【図27】シャーレ搬送作動説明図。FIG. 27 is an explanatory view of a petri dish transport operation.
【図28】シャーレ搬送作動説明図。FIG. 28 is an explanatory diagram of a petri dish transport operation.
【図29】シャーレ搬送作動説明図。FIG. 29 is an explanatory view of the petri dish transport operation.
【図30】シャーレ搬送作動説明図。FIG. 30 is an explanatory diagram of a petri dish transport operation.
A トレイ搬送部 B 穿孔部 C サンプリング塗抹部 D シャーレ搬送部 E クリーンフアン部 1 トレイ 2 シャーレ 3 穿孔棒 4 採取具 5 光電センサー 6 モーター 10 昇降枠 12 ヒーター 16 光電センサー 17 昇降ブロック 18 横バー 19 螺軸 31 台枠 32 モーター 35 シャーレ載置台 40 塗抹前のシャーレストック位置 41 塗抹後のシャーレストック位置 A tray transport unit B punching unit C sampling smearing unit D petri dish transporting unit E clean fan unit 1 tray 2 petri dish 3 punching rod 4 sampling tool 5 photoelectric sensor 6 motor 10 lifting frame 12 heater 16 photoelectric sensor 17 lifting block 18 horizontal bar 19 screw Axis 31 Base frame 32 Motor 35 Petri dish mounting table 40 Petri dish stock position before smearing 41 Petri dish stock position after smearing
───────────────────────────────────────────────────── フロントページの続き (72)発明者 岡崎 仁志 神奈川県相模原市上鶴間48−5 (72)発明者 桑水流 守 埼玉県川越市南大塚1265−153 (72)発明者 千葉 正兄 神奈川県多摩区三田4−2−4 生田マン ション (72)発明者 中西 進 埼玉県所沢市若狭4−2471−11 ─────────────────────────────────────────────────── --- Continuation of the front page (72) Inventor Hitoshi Okazaki 48-5 Kamizuruma, Sagamihara City, Kanagawa Prefecture (72) Inventor Mamoru Kuwamizu 1265-153 Minami-Otsuka, Kawagoe City, Saitama Prefecture (72) Inventor Masahiro Chiba, Kanagawa Prefecture 4-2-4 Mita, Tama-ku Ikuta Mansion (72) Inventor Susumu Nakanishi 4-2471-11 Wakasa, Tokorozawa, Saitama Prefecture
Claims (2)
穿孔した後、自動で加熱滅菌された採取具で検体を採取
して、培地の入ったシャーレに検体を自動で連続に塗抹
することを特徴とする検体の塗抹方法。1. A product is pierced with a piercing rod that is automatically heat-sterilized, and then a sample is collected with a sampling tool that is automatically heat-sterilized, and the sample is automatically and continuously smeared on a petri dish containing medium. A method for smearing a specimen, which is characterized in that
を穿孔する加熱ヒーター付き穿孔棒手段と、穿孔箇所か
ら検体を採取する加熱ヒーター付き採取具手段と、採取
具によって検体を採取し、培地の入ったシャーレに塗抹
する手段と、該塗抹後のシャーレおよび塗抹前のシャー
レを搬送する手段を備えたことを特徴とする自動連続塗
抹装置。2. A conveyor means for transporting the product, a piercing rod means with a heater for piercing the product, a collecting tool means with a heating heater for collecting a sample from a piercing point, and a sample for collecting a sample with the collecting tool, and a culture medium. An automatic continuous smearing device comprising means for smearing a petri dish containing the, and means for transporting the petri dish after smearing and the petri dish before smearing.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP5219548A JPH0767695A (en) | 1993-09-03 | 1993-09-03 | Method for smearing specimen and device therefor |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP5219548A JPH0767695A (en) | 1993-09-03 | 1993-09-03 | Method for smearing specimen and device therefor |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH0767695A true JPH0767695A (en) | 1995-03-14 |
Family
ID=16737232
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP5219548A Pending JPH0767695A (en) | 1993-09-03 | 1993-09-03 | Method for smearing specimen and device therefor |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPH0767695A (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8408860B2 (en) | 2007-01-12 | 2013-04-02 | Labtech Systems Limited | Method and apparatus for orientating a solid growth culture medium plate |
US8691558B2 (en) | 2007-01-12 | 2014-04-08 | Lbt Innovations Limited | Method and apparatus for inoculating and streaking a medium in a plate |
US9029129B2 (en) | 2007-01-12 | 2015-05-12 | Lbt Innovations Limited | Streaking applicator cartridge and a system for connecting same to a streaking apparatus |
US9983308B2 (en) | 2007-01-12 | 2018-05-29 | Lbt Innovations Limited | Method and apparatus for locating the surface of solid growth culture media in a plate |
-
1993
- 1993-09-03 JP JP5219548A patent/JPH0767695A/en active Pending
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8408860B2 (en) | 2007-01-12 | 2013-04-02 | Labtech Systems Limited | Method and apparatus for orientating a solid growth culture medium plate |
US8691558B2 (en) | 2007-01-12 | 2014-04-08 | Lbt Innovations Limited | Method and apparatus for inoculating and streaking a medium in a plate |
US8696294B2 (en) | 2007-01-12 | 2014-04-15 | Lbt Innovations Limited | Method and apparatus for orientating a solid growth culture medium plate |
US9029129B2 (en) | 2007-01-12 | 2015-05-12 | Lbt Innovations Limited | Streaking applicator cartridge and a system for connecting same to a streaking apparatus |
US9914953B2 (en) | 2007-01-12 | 2018-03-13 | Labtech Systems Ltd | Method and apparatus for inoculating and streaking a medium in a plate |
US9939357B2 (en) | 2007-01-12 | 2018-04-10 | Lbt Innovations Limited | System for the connection of a loaded cartridge to a cartridge holder |
US9983308B2 (en) | 2007-01-12 | 2018-05-29 | Lbt Innovations Limited | Method and apparatus for locating the surface of solid growth culture media in a plate |
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