JPH0751057A - Material containing proliferation promoting substance for lactic acid bacteria and bifidus bacteria - Google Patents
Material containing proliferation promoting substance for lactic acid bacteria and bifidus bacteriaInfo
- Publication number
- JPH0751057A JPH0751057A JP5199364A JP19936493A JPH0751057A JP H0751057 A JPH0751057 A JP H0751057A JP 5199364 A JP5199364 A JP 5199364A JP 19936493 A JP19936493 A JP 19936493A JP H0751057 A JPH0751057 A JP H0751057A
- Authority
- JP
- Japan
- Prior art keywords
- lactic acid
- acid bacteria
- extract
- bacteria
- promoting substance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、乳酸菌およびビフィズ
ス菌の増殖促進物質含有物に関する。さらに具体的に
は、本発明は、海洋性微細藻類の藻体からの抽出物から
なる、発酵乳などの食品製造に利用する乳酸菌およびビ
フィズス菌の増殖促進物質含有物に関する。腸内の有用
菌として知られている乳酸菌は、牛乳あるいは還元脱脂
乳中では増殖しにくく、従って十分な増殖をさせるため
には増殖促進物質を添加する必要があることは周知の事
実である。さらに、こういった増殖促進物質を使用し、
乳酸菌を利用した発酵乳などの食品を製造する場合に
は、この増殖促進物質が強い風味を持たず、しかも広範
囲の乳酸菌種に対して強い活性を持つことが望まれる。TECHNICAL FIELD The present invention relates to a substance containing a growth promoting substance for lactic acid bacteria and bifidobacteria. More specifically, the present invention relates to a lactic acid bacterium and bifidobacteria growth-promoting substance-containing substance, which comprises an extract from an algal body of a marine microalgae and is used in the production of food such as fermented milk. It is a well-known fact that lactic acid bacteria, which are known as useful bacteria in the intestine, are difficult to grow in cow's milk or reduced skim milk, and therefore a growth promoting substance needs to be added in order to achieve sufficient growth. In addition, using these growth promoting substances,
When producing foods such as fermented milk using lactic acid bacteria, it is desired that this growth promoting substance does not have a strong flavor and has a strong activity against a wide range of lactic acid bacterial species.
【０００２】[0002]
【従来の技術】このような状況から、各種の増殖促進物
質が探索され、提案されているが、これらはいずれも何
らかの点で十分に満足すべきものとは言い難い。たとえ
ば、乳酸菌の増殖促進物質のうち、多くの菌種について
比較的活性が高いものとして公知となっている酵母エキ
ス、ペプトン、コーンスティープリカー、ムチンおよび
パンクレアチンは、いずれも独特の異臭を持つことか
ら、発酵乳等の食品製造への利用においては、使用方法
と使用量が大きく制限されざるを得なかった。また、ニ
ンジンエキス中の活性本体の一部とされるパンテチンな
どは、強い風味を持たないものの、乳酸菌の種類によっ
ては、ほとんど活性を示さないものがあるので、その使
用範囲が制約されていた。一方、強い風味を持たず、広
範囲の菌種に対して活性を示す乳酸菌増殖促進物質とし
ては、麦芽エキスが用いられている。乳酸菌が食品添加
用の菌として有用なものであるところから、その増殖促
進物質としての麦芽エキスは強い風味を持たないという
点で有望なものであるが、麦芽エキスは、どの乳酸菌に
対しても比較的活性が低く、乳酸菌を十分に増殖させる
ためには添加量が多くなるという欠点を持っている。2. Description of the Related Art Under these circumstances, various growth promoting substances have been searched and proposed, but it is difficult to say that all of them are sufficiently satisfactory in some respects. For example, among the growth-promoting substances of lactic acid bacteria, yeast extract, peptone, corn steep liquor, mucin and pancreatin, which are known to have relatively high activity for many bacterial species, all have a unique offensive odor. Therefore, in the use of fermented milk for food production, the method of use and the amount used have to be greatly limited. In addition, pantethine and the like, which are a part of the active substance in carrot extract, do not have a strong flavor, but depending on the type of lactic acid bacterium, there are some that show little activity, so the range of use was limited. On the other hand, malt extract is used as a lactic acid bacterium growth promoting substance which has no strong flavor and is active against a wide range of bacterial species. Since lactic acid bacteria are useful as bacteria for food additives, malt extract as a growth promoting substance is promising in that it does not have a strong flavor, but malt extract is suitable for any lactic acid bacterium. It has a relatively low activity and has the drawback that the amount added is large in order to sufficiently grow lactic acid bacteria.
【０００３】[0003]
【発明が解決しようとする課題】乳酸菌の増殖促進物質
として、酵母エキス、ペプトン、麦芽エキス、クロレラ
エキスなど多くの物質が提案されているが、いずれも独
特の異臭を持つために使用方法と使用量が制限されるこ
とや、乳酸菌に対して活性が低いなど、多くの解決すべ
き技術的課題があり、本発明の目的はこれを解決するこ
とにある。Many substances such as yeast extract, peptone, malt extract, and chlorella extract have been proposed as growth-promoting substances for lactic acid bacteria. There are many technical problems to be solved, such as limited amount and low activity against lactic acid bacteria, and an object of the present invention is to solve these problems.
【０００４】[0004]
【課題を解決するための手段】本発明者らは、海洋性微
細藻類の藻体抽出物の乳酸菌への増殖促進活性の研究を
行なったところ、クリプテコディニウム・コーニーの藻
体からの抽出物が顕著に乳酸菌の増殖を促進することを
見い出し、本発明を完成した。本発明は、上述の問題点
を持たない乳酸菌の増殖促進物質として、海洋性微細藻
類であるクリプテコディニウム・コーニーの藻体からの
抽出物を乳酸菌の増殖促進物質として提供することにあ
る。すなわち、本発明は、海洋性微細藻類クリプテコデ
ィニウム・コーニー（Crypthecodinium cohnii）の藻体
の抽出物よりなる、乳酸菌増殖促進物質含有物を提供す
る。さらに、前記藻体抽出物が藻体から温水ないし熱水
を用いて抽出した抽出物である上記に記載の乳酸菌増殖
促進物質含有物を提供する。また、前記藻体抽出物が藻
体から水溶性アルコール含有温水を用いて抽出した抽出
物である上記に記載の乳酸菌増殖促進物質含有物をも提
供する。[Means for Solving the Problems] The inventors of the present invention conducted a study on the growth promoting activity of an alga body extract of marine microalgae to lactic acid bacteria. As a result, the extraction of Crypthecodinium cornie from alga body was conducted. It was found that the product markedly promotes the growth of lactic acid bacteria and completed the present invention. The present invention provides, as a growth promoting substance for lactic acid bacteria, which does not have the above-mentioned problems, an extract from an algal body of Crypthecodinium cohnii, which is a marine microalgae, as a growth promoting substance for lactic acid bacteria. That is, the present invention provides a lactic acid bacteria growth-promoting substance-containing substance, which comprises an extract of an alga body of the marine microalga Crypthecodinium cohnii. Further, there is provided the lactic acid bacterium growth promoting substance-containing material as described above, wherein the alga body extract is an extract extracted from an alga body using warm water or hot water. Also provided is the lactic acid bacterium growth promoting substance-containing material as described above, wherein the alga body extract is an extract extracted from alga body using hot water containing a water-soluble alcohol.
【０００５】（作用）本発明において利用する微生物
は、ドコサヘキサエン酸を生成する海洋性微細藻類であ
ればよく、たとえば、クリプテコディニウム属、特に、
クリプテコディニウム・コーニー種などがある。具体的
には、クリプテコディニウム・コーニーＡＴＣＣ３００
２１、３０５４３、３０５５６、３０５７１、３０５７
２、３０５７５、５００５１、５００５３、５００５
５、５００５６、５００５８、５００６０等が挙げられ
る。特に、クリプテコディニウム・コーニーＡＴＣＣ３
００２１株は、ＤＨＡの産生量が高いので好ましい。こ
れらは公知の藻類であり、たとえばＡＴＣＣ（American
Type Culture Collection）などの保存機関より入手可
能である。(Action) The microorganism used in the present invention may be any marine microalgae that produces docosahexaenoic acid, for example, Crypthecodinium, particularly,
There are Crypthecodinium Cornie species. Specifically, Crypthecodinium Corney ATCC300
21, 30543, 30556, 30571, 3057
2, 30575, 50051, 50053, 5005
5, 50056, 50058, 50060 and the like. In particular, Crypthecodinium Cornie ATCC3
The strain 0021 is preferable because it produces a high amount of DHA. These are known algae, such as ATCC (American
It is available from storage organizations such as the Type Culture Collection).
【０００６】本発明に用いる藻類の藻体から抽出物は、
海洋性微細藻類を培養し、培養液から得られた藻体をそ
のまま湿潤藻体で、あるいは、乾燥させて乾燥藻体とし
た後、藻体から水またはアルコール類等を抽出剤として
用いて抽出して得ることができる。海洋性微生物の培養
は、以下の培地および方法を使用する。本発明において
利用する藻類の培養のための培地としては、この藻類が
良好に成育できる培地であればいかなる組成の培地も使
用できる。培養方法としては、静置培養、液体振盪培
養、液体タンク培養、回分培養、流加培養、連続培養な
どいかなる培養方法でもよい。使用する藻体としては、
培養終了後の培養液からの採取、あるいは濾過、遠心分
離などによる回収後の湿生藻体、藻体ペーストあるいは
加熱乾燥品、凍結乾燥品、噴霧乾燥品などの乾燥藻体で
あってもよい。The extract from the algal bodies of algae used in the present invention is
After culturing marine microalgae, the alga bodies obtained from the culture solution are used as wet alga bodies as they are, or after being dried to make dried alga bodies, extracted from the alga bodies using water or alcohols as an extractant. You can get it. Culture of marine microorganisms uses the following media and methods. As a medium for culturing algae used in the present invention, a medium having any composition can be used as long as the medium can grow well. The culture method may be any culture method such as static culture, liquid shaking culture, liquid tank culture, batch culture, fed-batch culture, and continuous culture. As the algal bodies used,
It may be a dried alga body such as a wet alga body, an alga body paste or a heat-dried product, a freeze-dried product, a spray-dried product, etc., which has been collected from the culture solution after the culturing, or collected by filtration, centrifugation or the like. .
【０００７】藻体からの抽出物の抽出方法としては、温
水、熱水あるいは水溶性アルコール含有温水を抽出溶媒
として使用して抽出する。ここで、温水とは、常圧で１
５〜６０℃、熱水とは、６０℃以上を意味する。抽出は
加圧して行ってもよく、抽出温度は、抽出圧力により異
なるが、常圧下での抽出温度は１５〜１００℃で行なう
ことができるが、６０〜９０℃が最適である。さらに、
加圧下での抽出では、１２０℃、１．２ｋｇ／ｃｍ2 、
１５分の加圧・オートクレーブ抽出も可能である。抽出
時間は、温度条件により異なるが、１０分〜１２時間、
特に、３０〜１２０分間行うのが好ましい。ただし、１
００℃、３時間以上などの高温・長時間の抽出は乳酸菌
増殖促進物質としての活性の低下を生じ、好ましくな
い。抽出溶媒としては水および水にエチルアルコール、
メチルアルコール、イソプロピルアルコールなど水溶性
の溶媒を添加しても使用できるが、食品用としてはエチ
ルアルコールが好ましい。アルコール濃度は、１０〜６
０％とするのが好ましい。抽出時のｐＨは、６．０〜
８．５の中性付近が好ましい。得られた抽出物は、その
ままで乳酸菌の増殖を促進する活性を示す。As a method for extracting the extract from the algal cells, hot water, hot water or hot water containing a water-soluble alcohol is used as an extraction solvent for extraction. Here, warm water is 1 at normal pressure.
5-60 degreeC and hot water mean 60 degreeC or more. The extraction may be carried out under pressure, and the extraction temperature varies depending on the extraction pressure, but the extraction temperature under normal pressure can be carried out at 15 to 100 ° C., but 60 to 90 ° C. is optimal. further,
For extraction under pressure, 120 ° C, 1.2 kg / cm 2 ,
Pressurization for 15 minutes and autoclave extraction are also possible. The extraction time varies depending on the temperature conditions, but is 10 minutes to 12 hours,
Especially, it is preferable to carry out for 30 to 120 minutes. However, 1
Extraction at 00 ° C. for 3 hours or more at a high temperature for a long time unfavorably causes a decrease in activity as a lactic acid bacterium growth promoting substance. Water and ethyl alcohol in water as the extraction solvent,
Although it can be used by adding a water-soluble solvent such as methyl alcohol or isopropyl alcohol, ethyl alcohol is preferable for food use. Alcohol concentration is 10-6
It is preferably 0%. The pH at the time of extraction is 6.0 to
Around 8.5 neutrality is preferable. The obtained extract shows the activity of promoting the growth of lactic acid bacteria as it is.
【０００８】さらに、この抽出物を用いて乳酸菌の培養
を行う。本発明に用いる乳酸菌およびビフィズス菌は、
糖を乳酸発酵する細菌であればいかなる細菌であっても
よい。具体的な例としては、乳酸菌として、ラクトバチ
ルス（Lactobacillus)属、ストレプトコッカス(Strepto
coccus )属、ビフィズス菌として、ビフィドバクテリウ
ム(Bifidobacterium) 属等に属するもの、特に、ビフィ
ドバクリウム・ロンガム(Bifidobacterium longum)種、
ビフィドバクリウム・インファンテイス(Bifidobacteri
um infantis)種、ビフィドバクリウム・ブレベ(Bifidob
acterium breve) 種、ラクトバチルス・カゼイ(Lactoba
cillus casei) 種、ラクトバチルス・ブルガリカス(Lac
tobacillus bulgaricus)種、ストレプトコッカス・ラク
ティス(Streptococcus lactis)種等が挙げられ、特に、
ラクトバチルス・カゼイ、ビフィドバクリウム・ロンガ
ム、ビフィドバクリウム・インファンテイス、ビフィド
バクリウム・ブレベ等が好ましい。Further, lactic acid bacteria are cultured using this extract. Lactic acid bacteria and bifidobacteria used in the present invention,
Any bacterium may be used as long as it is a lactic acid-fermenting bacterium. As a specific example, Lactobacillus (Lactobacillus) genus, Streptococcus (Strepto)
genus coccus), as a Bifidobacterium, those belonging to the genus Bifidobacterium (Bifidobacterium), in particular, Bifidobacurium longum (Bifidobacterium longum) species,
Bifidobacteri
um infantis), Bifidob
acterium breve), Lactobacillus casei (Lactoba
cillus casei), Lactobacillus bulgaricus (Lac
tobacillus bulgaricus) species, Streptococcus lactis (Streptococcus lactis) species and the like, in particular,
Lactobacillus casei, bifidobacurium longum, bifidobacurium infantace, bifidobacurium brevet and the like are preferable.
【０００９】次に、上述の乳酸菌を、乳酸菌増殖促進物
質を含有する培地に接種して培養する。乳酸菌の培養に
用いる培地は、通常の乳酸菌が生育できる培地であれば
いかなる培地でもよいが、特に、乳酸菌飲料または乳酸
菌製品を製造する場合、培地中に、５〜２０重量％の還
元脱脂乳などの脱脂乳を含有するのが好ましい。乳酸菌
の培養方法は、通常、嫌気培養によって行う。培養器を
嫌気的条件、すなわち、乳酸菌が分子状酸素に触れない
ようにするためには、ｉ）培地を容器内に満たした後、
加熱し溶存酸素を取り除いてから乳酸菌を加える、ｉ
ｉ）耐圧性の容器で真空あるいは減圧にして培養する、
ｉｉｉ）培地に還元剤を添加する等の方法を用いればよ
い。乳酸菌増殖促進物質として、上記海洋性微細藻類か
らの抽出物を使用することができ、これに、さらに、そ
の他の既知の高活性の乳酸菌増殖促進物質を添加しても
よい。乳酸菌増殖促進物質である海洋性微細藻類の菌体
から得られた抽出物の添加量は、培地１００ｍｌ当たり
０．０１〜２．０ｍｌ（抽出物の乾燥重量で０．０１〜
２．０重量％）であるのが好ましい。その他の高活性の
乳酸菌増殖促進物質としては、酵母エキス、ペプトン、
コーンスティープリカー等が挙げられ、これらと本発明
の乳酸菌増殖促進物質とを併用してもよい。培養開始時
の乳酸菌の添加量は、培地１ｍｌ当たり１０6 〜１０7
個の細胞になるように乳酸菌の菌体を加えればよい。乳
酸菌培養用の培地、乳酸菌および海洋性微細藻類の藻体
からの抽出物を含有する混合液を以下の培養条件で得る
ことができる。乳酸菌の培養条件として、ｐＨは、培養
により産成する乳酸などの酸により培養の過程で変化す
るが、培養開始時に、６．０〜７．５である。培養温度
は、３５℃付近である。培養時間は、１６時間程度であ
り、従来の乳酸菌増殖促進物質を添加した場合に比べて
短く、本発明の乳酸菌増殖促進物質を添加した場合、短
期間の培養で乳酸菌の高い酸度を得ることができる。乳
酸菌の培養後の増加酸度を０．１Ｎ ＮａＯＨで滴定す
ることにより測定した。この増加酸度を乳酸菌増殖促進
活性の指標とした。本発明の乳酸菌の増加酸度は、９．
０〜１０．５であった。これは、通常の乳酸菌増殖促進
物質の増加酸度が、４．１〜６．８であるのに比べて高
い。Next, the above-mentioned lactic acid bacterium is inoculated into a medium containing a lactic acid bacterium growth promoting substance and cultured. The medium used for culturing the lactic acid bacterium may be any medium as long as it can grow ordinary lactic acid bacteria, but particularly in the case of producing a lactic acid bacterium drink or a lactic acid bacterium product, 5 to 20% by weight of reduced skim milk or the like is contained in the medium. It is preferable to contain non-fat milk. The lactic acid bacteria are usually cultured by anaerobic culture. In order to keep the incubator under anaerobic conditions, that is, to prevent lactic acid bacteria from contacting molecular oxygen, i) after filling the medium with a medium,
Add lactic acid bacteria after heating to remove dissolved oxygen, i
i) culture in a pressure-resistant container under vacuum or reduced pressure,
iii) A method such as adding a reducing agent to the medium may be used. As the lactic acid bacterium growth promoting substance, an extract from the above-mentioned marine microalgae can be used, and other known highly active lactic acid bacterium growth promoting substance may be further added thereto. The amount of the extract obtained from the cells of the marine microalgae, which is a lactic acid bacterium growth promoting substance, is 0.01 to 2.0 ml per 100 ml of the medium (0.01 to 2.0 in terms of dry weight of the extract).
2.0% by weight) is preferable. Other highly active lactic acid bacteria growth-promoting substances include yeast extract, peptone,
Examples thereof include corn steep liquor, and these may be used in combination with the lactic acid bacterium growth promoting substance of the present invention. The amount of lactic acid bacteria added at the start of culture is 10 6 to 10 7 per 1 ml of the medium.
The cells of lactic acid bacteria may be added so as to form individual cells. A mixed solution containing a medium for lactic acid bacterium culture, a lactic acid bacterium, and an extract from an alga body of a marine microalga can be obtained under the following culture conditions. As a culturing condition for lactic acid bacteria, the pH is 6.0 to 7.5 at the start of culturing, although it changes in the course of culturing due to acids such as lactic acid produced by culturing. The culture temperature is around 35 ° C. The culturing time is about 16 hours, which is shorter than that in the case of adding the conventional lactic acid bacterium growth promoting substance. When the lactic acid bacterium growth promoting substance of the present invention is added, a high acidity of the lactic acid bacterium can be obtained by short-term culturing. it can. The increased acidity of lactic acid bacteria after culture was measured by titrating with 0.1N NaOH. This increased acidity was used as an index of lactic acid bacterium growth promoting activity. The increased acidity of the lactic acid bacterium of the present invention is 9.
It was 0-10.5. This is higher than the increased acidity of a normal lactic acid bacterium growth promoting substance being 4.1 to 6.8.
【００１０】得られた培養液は、そのまま乳酸菌飲料、
乳酸菌添加食品として使用することができる。このよう
にして得られた培養液は、乳酸菌増殖促進物質として、
海洋性微細藻類由来の抽出物を用いるため、従来の乳酸
菌増殖促進物質と異なり異臭を有さない。また、得られ
た培養液から、ろ過、遠心分離等により乳酸菌を分離
し、そのまま乳酸菌の湿潤菌体とするか、または、凍結
乾燥、噴霧乾燥等により乳酸菌の乾燥菌体としてもよ
い。The obtained culture solution is directly used as a lactic acid bacterium beverage,
It can be used as a food containing lactic acid bacteria. The culture solution thus obtained, as a lactic acid bacterium growth promoting substance,
Since an extract derived from marine microalgae is used, it does not have an offensive odor unlike conventional lactic acid bacteria growth promoting substances. Alternatively, lactic acid bacteria may be separated from the obtained culture broth by filtration, centrifugation or the like to obtain wet microbial cells of lactic acid bacteria as it is, or dried lactic acid bacteria may be obtained by freeze-drying, spray drying or the like.
【００１１】[0011]
【実施例】以下、実施例により本発明をさらに具体的に
説明する。 （実施例１）クリプテコディニウム・コーニーＡＴＣＣ
３００２１株の培養で得た凍結乾燥藻体１００ｇを７０
℃の熱水１リットルに加え、撹拌しながら１時間可溶物
を抽出し、この抽出物を１０℃、８０００×ｇで１５分
間遠心分離した後、抽出上清（抽出液）０．８１Ｌを得
た。得られた上清を１．２リットルの９５％エタノール
と混合し、一晩静置した。さらに、この上清混合物をデ
カンテーションして沈澱部を分離（除去）し、これを真
空凍結乾燥によって乾燥して、クリプテコディニウム・
コーニーの熱水抽出物（約０．２ｇ）とした。この抽出
物あるいはその他の表１に示す高活性の乳酸菌増殖促進
物質を、固形分濃度１０％の還元脱脂乳１リットルの入
った培養器に、乾燥重量で０．２重量％となるように各
々添加し、さらにビフィドバクテリウム・ロンガム（Bi
fidobacterium longum) 菌を培地１ｍｌ当たり５×１０
6 個程度接種して、３５℃で、１６時間嫌気培養した。
培養後の増加酸度を培養液１ｍｌに対して、０．１Ｎ
ＮａＯＨで滴定することにより測定した。この結果、下
記表１に示すように、本発明による藻体抽出物は、公知
の乳酸菌増殖促進物質のうち、特に活性が高いといわれ
ているものに比較しても、さらに高活性であることが分
かった。EXAMPLES The present invention will be described in more detail below with reference to examples. (Example 1) Crypthecodinium cornie ATCC
100 g of freeze-dried algae obtained by culturing 30021 strain
Add 1 liter of hot water at ℃, extract the soluble matter for 1 hour with stirring, centrifuge this extract at 8000 × g for 15 minutes at 10 ℃, and then extract 0.81 L of extraction supernatant (extract). Obtained. The obtained supernatant was mixed with 1.2 liters of 95% ethanol and allowed to stand overnight. Further, the supernatant mixture was decanted to separate (remove) a precipitate, and the precipitate was dried by vacuum freeze-drying to obtain cryptecodinium.
It was a hot water extract of coney (about 0.2 g). Each of the extract or other highly active lactic acid bacteria growth-promoting substances shown in Table 1 was placed in an incubator containing 1 liter of reduced skim milk having a solid content of 10% so that the dry weight was 0.2% by weight. Bifidobacterium longum (Bi
fidobacterium longum) 5 × 10 per 1 ml of medium
About 6 cells were inoculated and anaerobically cultured at 35 ° C for 16 hours.
Increased acidity after culturing was 0.1N per 1 ml of culture solution.
It was measured by titration with NaOH. As a result, as shown in Table 1 below, the alga body extract according to the present invention has higher activity than known lactic acid bacterium growth-promoting substances, which are said to have particularly high activity. I understood.
【００１２】（実施例２）実施例１と同様にして調整し
たクリプティコデニウム・コーニーの熱抽出物および市
販酵母エキスを、各々、固形分濃度１０重量％の還元脱
脂乳１リットルの入った培養器に、乾燥重量で０．２重
量％となるように添加後、さらに各々の反応器にビフィ
ドバクテリウム・ロンガム菌を培地１ｍｌ当たり５×１
０6 個程度接種して、両者とも発酵乳のｐＨが５．０程
度となるまで、３５℃で、嫌気培養した。また、ビフィ
ドバクテリウム・インファンテイス、ビフィドバクテリ
ウム・ブレベについても、ロンガム菌と同様の方法で培
養した。結果を、下記表２に示した。この発酵乳中に含
まれる乳酸菌の生菌体数を光岡らのＢＬ平板法により測
定したところ、乳酸菌の生菌体数については、クリプテ
コディニウム・コーニーの熱抽出物を添加したもの、お
よび、酵母エキスを添加したものとも３×１０9 個／ｍ
ｌ程度であった。一方、増加酸度については、本発明の
熱抽出物を添加したもののほうが、酵母エキスを添加し
たものに比べて高いので、本発明の抽出物を用いた乳酸
菌の培養の方が、乳酸菌の個々の菌体が乳酸を生成させ
る菌体の成熟度が高いと考えられる。さらに、酵母エキ
ス添加乳は、酵母エキス由来の強い異臭が感じられた
が、本発明に使用された抽出物の添加乳は異臭を持たな
かった。(Example 2) A heat extract of Crypticodenium cohnii and a commercially available yeast extract prepared in the same manner as in Example 1 were each added to 1 liter of reduced skim milk having a solid content of 10% by weight. After adding to the incubator so as to have a dry weight of 0.2% by weight, Bifidobacterium longum was added to each reactor at 5 × 1 per 1 ml of the medium.
About 0 6 were inoculated, and both were anaerobically cultured at 35 ° C until the pH of the fermented milk reached about 5.0. In addition, Bifidobacterium infantis and Bifidobacterium breve were also cultured in the same manner as for the longum bacterium. The results are shown in Table 2 below. When the viable cell count of lactic acid bacteria contained in this fermented milk was measured by the BL plate method of Mitsuoka et al., The viable cell count of lactic acid bacteria was determined by adding a heat extract of Crypthecodinium cohnii, and , 3 × 10 9 cells / m with yeast extract added
It was about 1. On the other hand, as for the increased acidity, the one to which the heat extract of the present invention was added is higher than the one to which the yeast extract was added, so that the lactic acid bacterium culture using the extract of the present invention was It is considered that the bacterial cells that produce lactic acid have high maturity. Furthermore, the yeast extract-added milk had a strong offensive odor derived from the yeast extract, but the extract-added milk used in the present invention had no offensive odor.
【００１３】 ＊ 添加濃度はすべて０．２重量％ ＊＊ 乳酸菌の培養液１ｍｌに対して、０．１Ｎ Ｎａ
ＯＨを用いた滴定値[0013] * Addition concentration is 0.2% by weight ** 0.1N Na per 1 ml of lactic acid bacterium culture solution
Titration value using OH
【００１４】 ＊ 添加濃度はすべて０．２重量％ ＊＊ 乳酸菌の培養液１ｍｌに対して、０．１Ｎ Ｎａ
ＯＨを用いた滴定値[0014] * Addition concentration is 0.2% by weight ** 0.1N Na per 1 ml of lactic acid bacterium culture solution
Titration value using OH
【００１５】[0015]
【発明の効果】以上に説明したように、本発明の藻体の
抽出物を含有する乳酸菌の培養液は、たとえば市販の各
種の麦芽エキス、酵母エキス、ペプトンに比べて乳酸菌
増殖促進作用が顕著に、たとえば２．０倍程度に増大し
ている。さらに、本発明の海洋性微細藻類の藻体からの
抽出物は、少量の添加で乳酸菌の増殖を促進する。な
お、本発明による抽出物の乳酸菌増殖促進物質は、強い
異臭を持たないので、そのまま乳酸菌飲料、乳酸菌添加
食品として使用できる。As described above, the culture solution of lactic acid bacteria containing the extract of alga of the present invention has a remarkable lactic acid bacterium growth promoting action as compared with, for example, various commercially available malt extracts, yeast extracts and peptones. In particular, it is increased to about 2.0 times. Furthermore, the extract of the marine microalgae of the present invention from the algal cells promotes the growth of lactic acid bacteria even when added in a small amount. Since the lactic acid bacterium growth promoting substance of the extract according to the present invention does not have a strong offensive odor, it can be directly used as a lactic acid bacterium drink or a lactic acid bacterium-added food.
フロントページの続き (51)Int.Cl.6 識別記号 庁内整理番号 ＦＩ 技術表示箇所 //(Ｃ１２Ｎ 1/38 Ｃ１２Ｒ 1:89） (Ｃ１２Ｐ 1/00 Ｃ１２Ｒ 1:89） Continuation of front page (51) Int.Cl. 6 Identification number Office reference number FI technical display area // (C12N 1/38 C12R 1:89) (C12P 1/00 C12R 1:89)
Claims (4)
ることを特徴とする乳酸菌およびビフィズス菌の増殖促
進物質含有物。1. A substance containing a growth promoting substance for lactic acid bacteria and bifidobacteria, which comprises an extract from an alga body of a marine microalga.
ないし熱水を用いて抽出した抽出物である請求項１に記
載の乳酸菌およびビフィズス菌の増殖促進物質含有物。2. The growth-promoting substance-containing material for lactic acid bacteria and bifidobacteria according to claim 1, wherein the extract from the alga is an extract extracted from the alga using warm water or hot water.
性アルコール含有温水で抽出した抽出物である請求項１
に記載の乳酸菌およびビフィズス菌の増殖促進物質含有
物。3. The extract from the alga body is an extract extracted from the alga body with warm water containing a water-soluble alcohol.
A lactic acid bacterium and bifidobacteria growth-promoting substance-containing product as described in 1.
ウム・コーニー（Crypthecodiniumcohnii）である請求
項１〜３のいずれかに記載の乳酸菌およびビフィズス菌
の増殖促進物質含有物。4. The growth-promoting substance-containing material for lactic acid bacteria and bifidobacteria according to claim 1, wherein the marine microalgae is Crypthecodinium cohnii.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5199364A JPH0751057A (en) | 1993-08-11 | 1993-08-11 | Material containing proliferation promoting substance for lactic acid bacteria and bifidus bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5199364A JPH0751057A (en) | 1993-08-11 | 1993-08-11 | Material containing proliferation promoting substance for lactic acid bacteria and bifidus bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0751057A true JPH0751057A (en) | 1995-02-28 |
Family
ID=16406534
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5199364A Pending JPH0751057A (en) | 1993-08-11 | 1993-08-11 | Material containing proliferation promoting substance for lactic acid bacteria and bifidus bacteria |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0751057A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006112364A1 (en) * | 2005-04-15 | 2006-10-26 | Dainippon Ink And Chemicals, Inc. | Hypotensive agent produced by cultivation of lactic acid bacterium |
| WO2006126476A1 (en) | 2005-05-27 | 2006-11-30 | Kabushiki Kaisha Yakult Honsha | Lactic acid bacteria fermented substance and fermented milk food product containing the same |
| KR20200082825A (en) * | 2018-12-31 | 2020-07-08 | 재단법인 전남생물산업진흥원 | Prebiotics composition comprising extract of Porphyra yezoensis as effective component and uses thereof |
-
1993
- 1993-08-11 JP JP5199364A patent/JPH0751057A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006112364A1 (en) * | 2005-04-15 | 2006-10-26 | Dainippon Ink And Chemicals, Inc. | Hypotensive agent produced by cultivation of lactic acid bacterium |
| JP4762987B2 (en) * | 2005-04-15 | 2011-08-31 | Ｄｉｃライフテック株式会社 | Antihypertensive agent obtained by lactic acid bacteria culture |
| WO2006126476A1 (en) | 2005-05-27 | 2006-11-30 | Kabushiki Kaisha Yakult Honsha | Lactic acid bacteria fermented substance and fermented milk food product containing the same |
| US9708579B2 (en) | 2005-05-27 | 2017-07-18 | Kabushiki Kaisha Yakult Honsha | Lactic acid bacteria fermented substance and fermented milk food product containing the same |
| KR20200082825A (en) * | 2018-12-31 | 2020-07-08 | 재단법인 전남생물산업진흥원 | Prebiotics composition comprising extract of Porphyra yezoensis as effective component and uses thereof |
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