JPH074277B2 - Method for producing sweetener - Google Patents

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to an intermediate sugar solution in a beet sugar manufacturing process or an ion chromatographic waste solution containing a large amount of raffinose which is simultaneously discharged when sucrose is efficiently recovered from the molasses by an ion chromatographic method. As it is, or after desalting and concentration, lactose is added, and β-galactosyltransferase is allowed to act on the sugar to enrich oligosaccharides that activate the growth of useful bacteria in the intestine, especially bifidobacteria. The present invention relates to a method of manufacturing a food.

[0002]

2. Description of the Related Art Since beet sugar solution contains raffinose, the raffinose concentration of the separated honey gradually increases and the decoction (crystallization of sucrose) is inhibited by repeated decoction.

In order to prevent this, in the conventional sugar beet factory,
Raffinose in the separated nectar of three-stage decoction is decomposed by the enzyme melibiase and the inhibition is suppressed as much as possible.
Raffinose accumulates in molasses because it is not decomposed by 0%.

On the other hand, it is already known that raffinose is a non-digestible oligosaccharide useful for bifidobacteria, but by positively utilizing this, the molasses with increased raffinose concentration is used. Β-fructosyl transferase to act on sucrose to convert sucrose to fructooligosaccharides, a method for producing a sweetener enriched with oligosaccharides (JP-A-61-9266) and molasses at a desalting plant without melibiase treatment, A method of converting β-fructosyltransferase into a raffinose fraction obtained by fractionating separated honey from two-stage decoction by ion chromatography to convert it to fructooligosaccharide (Japanese Patent Laid-Open No. 62-126951) or without conversion. A method for producing a sweetener containing a concentrated bifidobacteria-grown sugar source has been proposed (Japanese Patent Laid-Open No. 62-126951).

[0005]

[Problems to be Solved by the Invention] JP-A-62-12695
The method of condensing the sugar beet sugar solution desalted by the ion exchange resin disclosed in No. 1 repeatedly and concentrating the raffinose fraction obtained by fractionating the separated so-called bean jam is useful for bifidobacteria. Raffinose, which is an oligosaccharide, stops at 25-30% at best.

Further, the above-mentioned Japanese Patent Laid-Open Nos. 61-9266 and
The method of acting β-fructosyltransferase in molasses with high raffinose concentration disclosed in 62-126951 is to convert sucrose in molasses into fructooligosaccharide while decomposing sucrose. At the same time that the oligosaccharide content becomes high, the β-fructosyl transferase used has a possibility that raffinose is partially decomposed due to the nature of the enzyme and, depending on the conditions, may be considerably decomposed, and thus is not an efficient method.

[0007]

[Means for Solving the Problems] In view of the above situation, the present invention fractionates a sugar beet sugar solution by an ion chromatography method, and recovers a sucrose content, and then a waste solution containing a large amount of raffinose is directly or desalted. Lactose was added to the solution, and then β-
The present invention proposes a method for producing a sweetener which causes galactosyltransferase to act to convert sucrose in concentrated ion chromatography waste liquid into β-galactosyl sucrose.

Further, in the present invention, after the β-galactosyltransferase is acted on as described above, the sucrose in the concentrated ion chromatography waste liquid is converted into β-galactosyl sucrose and then concentrated and cooled again to be precipitated. It proposes a method for producing a sweetener, in which a precipitate of raffinose and β-galactosyl sucrose is separated and dried.

It is already known that β-galactosyl sucrose (isorafinose) is selectively assimilated by bifidobacteria, but according to the present invention, as described above, the ion chromatograph waste liquid containing a high concentration of raffinose. Alternatively, lactose was added to the desalted solution, and β-galactosyltransferase was allowed to act on the β-galactose to decompose β-galactose.
-By transferring the galactosyl group to sucrose, β-
Galactosyl sucrose is produced with a transfer rate of 50-60%.

On the other hand, since β-galactosyltransferase does not act on raffinose, raffinose is not decomposed. Therefore, the total with raffinose is 50
It can be a sweetener enriched with oligosaccharides useful for bifidobacteria exceeding%.

Here, as the sugar beet sugar solution, a sugar solution from a factory that uses the Steffen method, a sugar solution from a factory that uses the desalting method, and the like can be used.

These sugar solutions are fractionated by an ion chromatographic method for the purpose of increasing the purity of sucrose, the sucrose fraction is returned to the sugar production step, and one fraction / ion chromatography waste liquid is directly
Alternatively, it is desalted before use.

Here, as an apparatus used for the ion chromatography method, an ion chromatography apparatus by the simulated moving bed method or the new MIC method can be used.

Next, examples of the composition of the ion chromatographic supply liquid, the ion chromatographic waste liquid and the desalting liquid used in the present invention are shown below. Table 1 below uses the sugar solution of a factory that employs the Steffen method as the stock solution. Here, the syrup after the No. 2 decoction is used as the ion chromatography supply solution, and this syrup is used as the ion chromatography (simulated moving bed method-Mitsui The waste liquid after the high-purity sucrose fraction was collected by fractionation with an apparatus manufactured by Toatsu Kiko Co., Ltd. was used as an ion chromatography waste liquid. Furthermore, the ion chromatography waste liquid is
A cation-exchange resin and an OH-type anion exchange resin were passed through to remove the ash to obtain a desalting solution. The ion chromatographic feed used in the present invention is not limited to the syrup after the second decoction.

[0015]

[0016] Table 2 below uses the sugar solution of a factory that employs the desalting method as the stock solution. Here, A syrup is used as the ion chromatographic feed solution, and this syrup is used as the ion chromatograph (new MCI method-Mitsubishi Techno Engineering). Fractionation with a device (Aars Co., Ltd.) to collect a high-purity sucrose fraction and an inositol fraction is used as an ion chromatography waste liquid, and the waste liquid is further used as an H-type cation exchange resin and an OH-type anion exchange. A desalting solution was obtained by passing the solution through a resin to remove ash. The ion chromatographic supply liquid used in the present invention is not limited to A syrup.

[0017]

The ion chromatography waste liquid or its desalted liquid is concentrated to, for example, about B × 50 °, and then lactose is added to this liquid. The amount of lactose added is about 10 to 25 Wt%.

After the lactose is dissolved, β-galactosyltransferase is allowed to act. Β used in this invention
-Galactosyl transferase can be used as long as it decomposes lactose and transfers its β-galactosyl group to sucrose to produce β-galactosyl sucrose.

Such β-galactosyltransferase is present in plant seeds, as well as in Bacillus circulans and Escherichia coli.
(Escherichia coli), Lactobacillus bifidus (Lac
tobacillus biffidus) and other bacteria and Rhodothurula minuta
(Rhodotorula minuta), Rhodotorula lactosa (Rhodo
It is known to be produced by yeast such as torula lactosa). Of the above, available enzymes may be used.

The action conditions of β-galactosyltransferase are, for example, the above-mentioned lactose-added solution is adjusted to be weakly acidic (about pH 6.0), and β-galactosyltransferase is added thereto in an amount of about 1,000 to 5,000, followed by heating at about 40 ° C. for 15 hours. To some extent. As a result, a high-quality sweetener having a refreshing sweetness can be obtained.

When the enzyme is allowed to act without being desalted, the solution after the action of β-galactosyltransferase is passed through an H-type cation exchange resin and an OH-type anion exchange resin to remove the salt. You may salt.

[0023]

INDUSTRIAL APPLICABILITY In summary, according to the present invention, it is effective in activating bifidobacteria, which are useful bacteria in the intestine, such as raffinose and β-galactosyl sucrose, and a low content of non-digestible oligosaccharides. A functional sweetener of calories can be obtained.

[0024]

Embodiments of the present invention will be specifically described below. Example 1 Separation syrup after No. 3 decoction in a factory employing the desalting method was applied to an ion chromatograph (new MCI method-manufactured by Mitsubishi Techno Engineers) to obtain a high-purity sucrose fraction, an inositol fraction, and a raffinose fraction. It was fractionated into three components. The high-purity sucrose fraction was returned to the sugar-making step, and about 7% of the ash content of the raffinose fraction remained, so it was passed through an H-type cation exchange resin and an OH-type anion exchange resin to remove the ash content.

The desalted solution was concentrated to B × 50 ° to give a stock solution (Table 3), and then 20 g of lactose was added to 80 g of the stock solution.
After adjusting the pH to 6.0, 3,000 u of β-galactosidase (Boehringer Mannheim) was added and allowed to act at 40 ° C for 15 hours. As a result, raffinose, an oligosaccharide useful for Bifidobacterium as shown in Table 3, was obtained. β-galactosyl sucrose yielded 100 g of syrup with 50.05% solids.

[0026]

Note) β-galactosyl sucrose is
Since the peak at the time of analysis overlaps with raffinose, the total value of raffinose + galactosyl sucrose is displayed.

Example 2 Separation syrup after No. 2 sucrose in a factory adopting the Steffen method was applied to an ion chromatography (simulated moving bed method-Mitsui Toatsu Kiko Co., Ltd.) device to obtain a high-purity sucrose fraction and other components. Fractionated into two components. The high-purity sucrose fraction was returned to the process, and about 35% of the ash remained in the ion chromatography waste liquid.
After desalting in the same manner as above, as shown in Table 4, raffinose and β-galactosyl sucrose were obtained as syrup of 47.23% based on the solid content.

[0029]

Example 3 100 g of the reaction solution obtained in Example 2 was inactivated with an enzyme, further concentrated, allowed to cool to B × 80 ° and allowed to cool in a low temperature chamber, and the precipitated oligosaccharide precipitate was centrifuged. When dried, purity 89
25% of a light brown oligosaccharide dried product was obtained.

Claims (4)

[Claims] 1. A beet sugar solution is fractionated by an ion chromatography method, and a waste solution containing a large amount of raffinose after recovering the sucrose content is directly or after being desalted and concentrated, lactose is added,
Furthermore, by acting on β-galactosyltransferase,
A method for producing a sweetener, which comprises converting sucrose in a concentrated ion chromatography waste liquid into β-galactosyl sucrose to enrich oligosaccharides useful for Bifidobacterium. 2. A sugar beet liquid is fractionated by an ion chromatography method, and a raffinose-rich waste liquid after recovery of sucrose is concentrated as it is or after being desalted and then lactose is added, and β is further added. -Acting galactosyltransferase to convert the sucrose in the concentrated ion chromatography waste liquid into β-galactosyl sucrose, and then concentrating and cooling again. A method for producing a sweetener, comprising: 3. The method for producing a sweetener according to claim 1, wherein the beet sugar solution is a sugar solution of a factory which employs a desalting method. 4. The method for producing a sweetener according to claim 1 or 2, wherein the beet sugar solution is a sugar solution of a factory that employs the Steffen method.