JPH0734375Y2 - Instrument for measuring and measuring reaction of analyte - Google Patents

Instrument for measuring and measuring reaction of analyte

Info

Publication number
JPH0734375Y2
JPH0734375Y2 JP1990095859U JP9585990U JPH0734375Y2 JP H0734375 Y2 JPH0734375 Y2 JP H0734375Y2 JP 1990095859 U JP1990095859 U JP 1990095859U JP 9585990 U JP9585990 U JP 9585990U JP H0734375 Y2 JPH0734375 Y2 JP H0734375Y2
Authority
JP
Japan
Prior art keywords
frame
well
container
measuring
plate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP1990095859U
Other languages
Japanese (ja)
Other versions
JPH0453555U (en
Inventor
隆 鈴木
功夫 飯田
俊男 森山
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shino Test Corp
Original Assignee
Shino Test Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shino Test Corp filed Critical Shino Test Corp
Priority to JP1990095859U priority Critical patent/JPH0734375Y2/en
Publication of JPH0453555U publication Critical patent/JPH0453555U/ja
Application granted granted Critical
Publication of JPH0734375Y2 publication Critical patent/JPH0734375Y2/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Landscapes

  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)

Description

【考案の詳細な説明】 (産業上の利用分野) この考案は、被検体物質の反応測定検出器具に関する。DETAILED DESCRIPTION OF THE INVENTION (Industrial field of application) The present invention relates to a device for measuring and detecting a reaction of an analyte.

具体的には、生化学的或いは免疫学的測定に用いる反応
容器若しくは測定容器であり、ELISA(enzyme linked i
mmunosorbent assay ;酵素免疫測定法)用プレート又は
赤血球、ラテックス等の人工坦体を用いた粒子凝集反応
用プレートに係るものである。
Specifically, it is a reaction vessel or measurement vessel used for biochemical or immunological measurement, and is an ELISA (enzyme linked i
mmunosorbent assay) or a plate for particle agglutination reaction using an artificial carrier such as red blood cells and latex.

(従来の技術) 従来、この種のELISA用プレートは、フレームに8穴筒
を有するウェルを12列に、若しくは12穴筒を有するウェ
ルを8列に、若しくは16穴筒を有するウェルを6列に、
それぞれ着脱自在にしたもの等が知られている。
(Prior Art) Conventionally, this type of ELISA plate has 12 rows of wells having an 8-hole tube in the frame, 8 rows of wells having a 12-hole tube, or 6 rows of wells having a 16-hole tube. To
It is known that each is detachable.

(考案が解決しようとする課題) 上記従来技術のELISA用プレートは、フレームとウェル
は何れも硬質の熱可塑性合成樹脂なる同質材で形成さ
れ、しかも、この両者は相互に単なる嵌め込み構成であ
るため、相互間に摩擦、キシミ、ガタつき等が生じ、プ
レートリーダーの測定値のばらつきの原因となる。ま
た、相互に破損や損傷が起生しやすく、その上、ウェル
が外れにくいと同時に、振動や回転を与える機械にかけ
た場合に、フレームからウェルが飛び出してしまう等の
問題点が多々生じていた。
(Problems to be Solved by the Invention) In the above-mentioned conventional plate for ELISA, the frame and the wells are both made of the same material made of a hard thermoplastic synthetic resin, and both are simply fitted together. However, friction, creaking, and rattling occur between each other, which causes variations in the measured values of the plate reader. In addition, mutual damage and damage are likely to occur, and moreover, wells are difficult to come off, and at the same time, when subjected to a machine that gives vibration or rotation, wells often pop out from the frame. .

(課題を解決するための手段) この考案は、上記従来品の課題を解決する目的を以って
開発したものであり、これを具体的実施例として示した
図面の符号に基づいて説明する。
(Means for Solving the Problems) This invention was developed with the object of solving the problems of the conventional products described above, and will be described with reference to the reference numerals of the drawings shown as specific examples.

すなわち、本案は、熱可塑性合成樹脂製の硬質容器
(1)と、熱可塑性合成樹脂製の軟質フレーム(2)と
の組合せからなり、容器(1)は上面(3)を開口し、
底部(4)を閉塞した複数の円筒(5)を並列して連続
したウェル(6)を形成するとともに、該ウェル(6)
の両端の鍔(7)、(8)に穴(9)、(10)を穿ち、
かつ、鍔(7)、(8)の下部に突起(11)、(12)
を、並びに両側端の容器側に二つのリブ(13)、(1
4)、(15)、(16)をそれぞれ一体成形したものであ
り、フレーム(2)は、上下左右に連続する横桟(1
7)、(18)と、縦桟(19)、(20)とで四角枠を形成
するとともに、対向する横桟(17)、(18)は互いに内
側に少し湾曲し、間隔的に凹設した溝(21)、(22)並
びに該溝間に位置する凸面(23)、(24)にピン(2
5)、(26)を突設し、かつ、横桟(17)、(18)のそ
れぞれ複数の対向面(27)、(28)を弧状形に一体成形
したもので、フレーム(2)に複数のウェル(6)を並
列状に嵌め込んでなる被検体物質の反応測定検出器具を
提供するにある。
That is, the present invention comprises a combination of a hard container (1) made of thermoplastic synthetic resin and a soft frame (2) made of thermoplastic synthetic resin, and the container (1) has an upper surface (3) opened.
A plurality of cylinders (5) having closed bottoms (4) are arranged in parallel to form a continuous well (6), and the wells (6) are also formed.
Make holes (9) and (10) in the collars (7) and (8) at both ends of
Also, the protrusions (11), (12) are provided on the lower part of the collars (7), (8)
, And two ribs (13), (1
4), (15), and (16) are integrally molded, and the frame (2) has a horizontal rail (1
7), (18) and the vertical bars (19), (20) form a rectangular frame, and the opposing horizontal bars (17), (18) are slightly curved inward with each other and are provided at intervals. The groove (21), (22) and the convex surfaces (23), (24) located between the grooves.
5) and (26) are projected, and a plurality of horizontal crosspieces (17) and (18) are respectively integrally formed with a plurality of facing surfaces (27) and (28) in an arc shape. Another object of the present invention is to provide a reaction measurement and detection instrument for a substance to be tested, which comprises a plurality of wells (6) fitted in parallel.

(作用) この考案によると、フレーム(2)にウェル(6)を押
し込むと、フレーム(2)の溝(21)、(22)並びにピ
ン(25)、(26)のウェル(6)の突起(11)、(12)
並びに穴(9)、(10)がそれぞれ合致するとともに、
フレーム(2)の対向面(27)、(28)にウェル(6)
のリブ(13)、(14)、(15)、(16)が係合して相互
に嵌まり込む。このようにして、一般的には、フレーム
(2)に12列のウェル(6)を嵌め込むことによって、
96穴プレートが設定される。
According to the present invention, when the well (6) is pushed into the frame (2), the grooves (21) and (22) of the frame (2) and the projections of the wells (6) of the pins (25) and (26) are projected. (11), (12)
And holes (9) and (10) match,
Wells (6) on the facing surfaces (27) and (28) of the frame (2)
The ribs (13), (14), (15), (16) of the above are engaged and fitted into each other. Thus, generally by fitting 12 rows of wells (6) into the frame (2),
96-well plate is set.

そして、フレーム(2)は軟質の合成樹脂で、また、ウ
ェル(6)は硬質の合成樹脂でそれぞれ製されているの
で、ウェル(6)には保形性があり、そして、フレーム
(2)には柔軟性があり、かつ、滑りやすいから、フレ
ーム(2)にウェル(6)の嵌め込みが円滑に行なえ
る。
Since the frame (2) is made of a soft synthetic resin and the well (6) is made of a hard synthetic resin, the well (6) has a shape retention property, and the frame (2) is Since it is flexible and slippery, the well (6) can be smoothly fitted into the frame (2).

また、ウェル(6)の両側に設けた二つのリブ(13)、
(14)、(15)、(16)が垂直方向への縦振れのガタつ
きをなくし、フレーム(2)との嵌合作用を良好にする
とともに、一方の横桟(17)に設けたピン(25)と他方
の横桟(18)に設けたピン(26)が、ウェル(6)の中
心線上に位置しているのではないから、フレーム(2)
にウェル(6)を嵌め込んで、縦方向及び横方向から挾
み込み、振動を与える機械に設定しても、両方向性に対
処でき、ウェル(6)がフレーム(2)から飛び跳ねる
ことがなく、しかも、嵌め込みの際に方向性を間違える
こともない。
Also, two ribs (13) provided on both sides of the well (6),
The pins (14), (15), (16) eliminate the vertical runout in the vertical direction to improve the fitting action with the frame (2) and the pin provided on one of the horizontal bars (17). Since the pins (26) provided on the (25) and the other horizontal rail (18) are not located on the center line of the well (6), the frame (2)
Even if the well (6) is fitted to the machine, and it is set in a machine that vibrates vertically and horizontally and gives vibration, the bidirectionality can be dealt with and the well (6) does not jump from the frame (2). Moreover, there is no mistake in the directionality when fitting.

さらに、対向する横桟(17)、(18)は、互いに内側に
湾曲しているから、その中ほど間が最も狭く、縦桟(1
9)、(20)に向かうに従って僅かに広く形成されてお
り、しかも、フレーム(2)は軟質の合成樹脂製である
が故、設定されたウェル(6)の中ほどの部分が使用時
にフレーム(2)から外れにくく、また、両側部分のウ
ェル(6)を外れやすくして、着脱並びに廃棄の際に平
均化する作用を行なう。
Further, since the opposing horizontal rails (17) and (18) are curved inward with respect to each other, the space between them is the narrowest, and the vertical rail (1
9), it is formed slightly wider toward (20), and since the frame (2) is made of soft synthetic resin, the middle part of the set well (6) is the frame when used. (2) is hard to come off, and the wells (6) on both sides are made easy to come off so as to perform the function of averaging at the time of attachment / detachment and disposal.

なお、フレーム(2)から使用済みのウェル(6)を取
り外す場合は、プレートを裏返しにして、フレーム
(2)の両横桟(17)、(18)を引っ張りながらウェル
(6)の底部(4)を軽く押すことにより、容易に廃棄
することができる。
When the used well (6) is to be removed from the frame (2), the plate is turned upside down and the bottom portions of the well (6) (while pulling the horizontal crosspieces (17) and (18) of the frame (2) ( It can be easily discarded by pressing 4) lightly.

(実施例1) 12列設定用のフレーム(2)をアクリルブタジエンスチ
レン(ABS)を、また、ウェル(6)にポリスチレンを
それぞれ用いて本考案を製した。
Example 1 The present invention was manufactured by using acrylic butadiene styrene (ABS) as the frame (2) for setting 12 rows and polystyrene as the well (6).

フレーム(2)に12列のウェル(6)を設定した際に、
極めて円滑に行なえ、しかも、このプレートをプレート
ミキサーで攪拌したところ、ウェル(6)がフレーム
(2)から飛び跳ねることもなく、相互にキシムことも
なく、かつ、破傷することもなかった。
When setting 12 rows of wells (6) in the frame (2),
When the plate was agitated with a plate mixer, the well (6) did not bounce off the frame (2), did not sime each other, and was not damaged.

また、作用済みのウェル(6)をフレーム(2)から取
り外す際に、本体を裏返しにして、フレーム(2)の両
端を少し引っ張りながら、ウェル(6)の底部(4)を
軽く押したところ、ウェル(6)が容易に外れ、これを
廃棄することができた。
Also, when removing the wells (6) that have been acted on from the frame (2), the body is turned over and the bottoms (4) of the wells (6) are lightly pressed while slightly pulling both ends of the frame (2). , Well (6) was easily removed and could be discarded.

なお、ウェル(6)は8穴筒で、底面形状はU形のもの
を実施したが、12穴筒、16穴筒、24穴筒の平底、U底、
V底等にそれぞれ設計変更することもできる。
Although the well (6) was an 8-hole cylinder and had a U-shaped bottom surface, the 12-, 16-, and 24-well flat bottoms, U-bottoms,
It is also possible to change the design to the V bottom or the like.

(実施例2) 酵素免疫測定法への応用 この考案のプレートについて、ELISAを実施した。本案
のプレートは、12列設定用のフレーム(2)をアクリル
ブタジエンスチレン(ABS)で、また、ウェル(6)を
ポリスチレンで製したものを使用した。なお、ウェルの
底面形状はU型のものを使用した。
(Example 2) Application to enzyme immunoassay The plate of this invention was subjected to ELISA. The plate of the present invention used a frame (2) for setting 12 rows made of acrylic butadiene styrene (ABS) and a well (6) made of polystyrene. The bottom shape of the well was U-shaped.

市販の抗CRP抗血清(ATAB社製)を硫安塩析後、DEAE−
セルロースカラムクロマトグラフィーにて分画精製し
た。この精製抗CRP抗体(100μg/ml)を本案プレートに
100μl添加し、37℃にて5時間静置した。これに、1
(w/v)%BSA−PBS 200μlを加え、さらに5時間静置
後、液を捨ててから乾燥することにより、抗CRP抗体固
相化プレートを調製した。なお、対照として市販の分離
型プレート(ヌンク社製96ウェルU型モデュラープレー
ト)を同様に調製した。
Commercially available anti-CRP antiserum (manufactured by ATAB) was salted out with ammonium sulfate, and then DEAE-
It was fractionated and purified by cellulose column chromatography. Apply this purified anti-CRP antibody (100 μg / ml) to the plate
100 μl was added and the mixture was allowed to stand at 37 ° C. for 5 hours. To this
200 μl of (w / v)% BSA-PBS was added, the mixture was allowed to stand still for 5 hours, the liquid was discarded, and the plate was dried to prepare an anti-CRP antibody-immobilized plate. As a control, a commercially available separation type plate (96 well U type modular plate manufactured by Nunc) was prepared in the same manner.

CRP標準液(シノテスト社製)を1%BSA−PBSにて10段
階希釈したものを試料とした。POD標識抗体は、自社製
のものを使用した。試料50μlをプレートの各ウェルに
添加し、37℃にて3時間保存後、0.05% Tween−20−PB
Sで洗浄後、呈色液(ABTS)200μlを加え37℃で30分静
置した。これをプレートリーダー(コロナ電気製MTP−3
2)にて測定した。
A sample was prepared by diluting a CRP standard solution (manufactured by Shinotest Co., Ltd.) in 10 steps with 1% BSA-PBS. As the POD-labeled antibody, an in-house manufactured one was used. 50 μl of sample was added to each well of the plate and stored at 37 ℃ for 3 hours, then 0.05% Tween-20-PB
After washing with S, 200 μl of coloring solution (ABTS) was added, and the mixture was allowed to stand at 37 ° C. for 30 minutes. This is a plate reader (Corona Electric MTP-3
It was measured in 2).

この結果、本案のプレートは、市販の分離型プレートと
比べ、プレート洗浄操作中も、ガタつきは生ぜず、フレ
ームからウェルが飛び出してしまうようなことはなく、
プレートを裏返して、洗浄液を完全に切ると云った操作
も可能であった。また、ガタつきを生じないため、プレ
ートリーダーの測定値のバラツキも小さかった。さら
に、使用済みのウェルはプレートを裏返して、フレーム
の両端を引っ張りながらウェルの底部を軽く押すことに
より、容易に廃棄することができた。
As a result, the plate of the present invention does not cause rattling and does not cause the well to pop out of the frame during the plate washing operation, as compared with the commercially available separation-type plate.
It was also possible to turn the plate over and completely drain the washing solution. Further, since there was no rattling, there was little variation in the measured values of the plate reader. Moreover, the used wells could be easily discarded by turning the plate over and gently pushing on the bottom of the well while pulling on both ends of the frame.

(実施例3) 粒子凝集反応への応用 実施例2に使用したフレーム(2)とウェル(6)を使
用した。本案のプレートを用い、市販のHBs抗原検出用
試薬セロディアンティーHBsについてプレートの性能を
調べた。
(Example 3) Application to particle agglutination reaction The frame (2) and well (6) used in Example 2 were used. Using the plate of the present invention, the performance of the plate was investigated for the commercially available reagent for detecting HBs antigen, therodianty HBs.

本案のプレートの第1ウェルに血清希釈用液50μl、第
2〜第8ウェルまで25μlずつ滴下した。マイクロピペ
ットでHBs抗原陽性血清5μlを採って第1ウェルに入
れ、第1ウェルから第8ウェルまで倍々希釈した。対照
血球を第1ウェルに25μl、感作血球を第2〜第8ウェ
ルに25μl滴下して各ウェルの全量を50μlとした。こ
のプレートをプレートミキサー(トレイミキサー)にか
け、最大設定で1分間振とう後、常温にて1時間静置
し、凝集像を判定した。なお、対照に市販の96ウェルU
型プレートを使用した。
To the first well of the plate of the present invention, 50 µl of the serum dilution solution and 25 µl of each of the second to eighth wells were dropped. 5 μl of HBs antigen-positive serum was taken with a micropipette, placed in the first well, and then diluted from the first well to the eighth well. 25 μl of control blood cells were dropped in the first well and 25 μl of sensitized blood cells were dropped in the second to eighth wells to make the total volume of each well 50 μl. This plate was placed on a plate mixer (tray mixer), shaken for 1 minute at the maximum setting, and then allowed to stand at room temperature for 1 hour to determine an aggregated image. As a control, 96 well U
A mold plate was used.

この結果、本案におけるウェル(6)は、プレートミキ
サーを最大振動させても、相互にキシムこともなく、か
つ、破損することもなかった。また、異常なウェルの振
動も生ぜず、測定試薬がウェルからこぼれ出ることもな
かった。凝集の検出感度は、96ウェル市販プレートと差
を認めなかった。さらに、使用済みのプレートの取外し
も、実施例2と同様に容易であった。
As a result, the wells (6) in the present invention did not sime each other and were not damaged even when the plate mixer was vibrated to the maximum. Moreover, abnormal vibration of the well did not occur, and the measurement reagent did not spill out of the well. The detection sensitivity of aggregation did not differ from that of the 96-well commercial plate. Furthermore, the removal of the used plate was as easy as in Example 2.

(考案の効果) この考案によれば、フレーム(2)を軟質の合成樹脂製
とし、また、ウェル(6)を硬質の合成樹脂製とするこ
と、フレーム(2)の対向する横桟(17)、(18)間を
中ほどを狭くし、縦桟(19)、(20)に向かうに従って
徐々に少し広くすること、着脱用並びに方向性を持たな
い溝(21)、(22)、突起(11)、(12)、ピン(2
5)、(26)、穴(9)、(10)、リブ(13)、(1
4)、(15)、(16)等の結合によつて、ウェル(6)
のフレーム(2)に対する着脱並びに噛合が極めて円
滑、かつ、容易となり、設定、反応測定、検出、廃棄等
の操作が簡便である。また、操作中においても、損傷、
破壊、削れ、摩擦、キシミ等が生じにくく、しかも、振
れやガタつきがなく、嵌合が良好なため、回転・攪拌機
にかけても、フレーム(2)からウェル(6)が飛び跳
ねることはなく、精度の高い検査結果を得ることができ
る。
(Effect of the Invention) According to this invention, the frame (2) is made of soft synthetic resin, and the well (6) is made of hard synthetic resin. ), (18) is narrowed in the middle and gradually widened toward the vertical crosspieces (19), (20), for attachment / detachment and non-directional grooves (21), (22), protrusions. (11), (12), pin (2
5), (26), holes (9), (10), ribs (13), (1
By combining 4), (15), (16), etc., the well (6)
The attachment and detachment with respect to the frame (2) and the engagement are extremely smooth and easy, and the operations such as setting, reaction measurement, detection and disposal are simple. Also, during operation, damage,
The breakage, scraping, friction, stains, etc. do not occur easily, and since there is no runout or rattling and the fitting is good, the well (6) does not jump from the frame (2) even when it is rotated and stirred, and the accuracy is high. It is possible to obtain high inspection results.

【図面の簡単な説明】[Brief description of drawings]

第1図は説明的な斜視図、第2図は容器(ウェル)の平
面図、第3図は同じく説明的な縦断面図、第4図は同じ
く一端部の底面図、第5図は同じく他端部の底面図であ
る。 なお、図中符号(1)は容器、(2)はフレーム、
(3)は上面、(4)は底部、(5)は円筒、(6)は
ウェル、(7)及び(8)は鍔、(9)及び(10)は
穴、(11)及び(12)は突起、(13)、(14)、(1
5)、(16)はリブ、(17)及び(18)は横桟、(19)
及び(20)は縦桟、(21)及び(22)は溝、(23)及び
(24)は凸面、(25)及び(26)はピン、(27)及び
(28)は対向面を示す。
1 is an explanatory perspective view, FIG. 2 is a plan view of a container (well), FIG. 3 is an explanatory vertical sectional view of the same, FIG. 4 is a bottom view of one end, and FIG. 5 is the same. It is a bottom view of the other end. In the figure, reference numeral (1) is a container, (2) is a frame,
(3) is a top surface, (4) is a bottom portion, (5) is a cylinder, (6) is a well, (7) and (8) are flanges, (9) and (10) are holes, (11) and (12). ) Is a protrusion, (13), (14), (1
5) and (16) are ribs, (17) and (18) are cross rails, (19)
And (20) are vertical bars, (21) and (22) are grooves, (23) and (24) are convex surfaces, (25) and (26) are pins, and (27) and (28) are opposing surfaces. .

フロントページの続き (72)考案者 森山 俊男 群馬県桐生市東4丁目10番19号 (56)参考文献 実開 昭2−46599(JP,U)Front page continuation (72) Inventor Toshio Moriyama 4-10-19 Higashi, Kiryu-shi, Gunma Prefecture (56) Bibliography Sho 2-46599 (JP, U)

Claims (2)

【実用新案登録請求の範囲】[Scope of utility model registration request] 【請求項1】熱可塑性合成樹脂製の硬質容器(1)と、
熱可塑性合成樹脂製の軟質フレーム(2)とからなり、
容器(1)は、上面(3)を開口し、底部(4)を閉塞
した複数の円筒(5)を並列して連続したウェル(6)
を形成するとともに、該ウェル(6)の両端の鍔
(7)、(8)に少なくとも一方がウェル(6)の中心
線上に位置しない穴(9)、(10)を穿ち、かつ、鍔
(7)、(8)の下部に突起(11)、(12)を、並びに
両側端の容器側に二つのリブ(13)、(14)、(15)、
(16)をそれぞれ一体成形したものであり、フレーム
(2)は、上下左右に連続する横桟(17)、(18)と、
縦桟(19)、(20)とで四角枠を形成するとともに、対
向する横桟(17)、(18)は互いに内側に少し湾曲し、
間隔的に凹設した溝(21)、(22)並びに該溝間に位置
する凸面(23)、(24)にピン(25)、(26)を突設
し、かつ、横桟(17)、(18)のそれぞれ複数の対向面
(27)、(28)を弧状形に一体成形したもので、容器
(1)の突起(11)、(12)をフレーム(2)の溝(2
1)、(22)に、穴(9)、(10)をピン(25)、(2
6)にそれぞれ嵌め込み、同時にウェル(6)の両端円
筒(5)が弧状形に沿って緩合するとともに、リブ(1
3)、(14)、(15)、(16)が対向面(27)、(28)
に係合することにより、フレーム(2)に複数の容器
(1)を並列状に組合せてなる被検体物質の反応測定検
出器具。
1. A hard container (1) made of thermoplastic synthetic resin,
It consists of a soft frame (2) made of thermoplastic synthetic resin,
The container (1) has a well (6) in which a plurality of cylinders (5) whose upper surface (3) is open and whose bottom (4) is closed are arranged in parallel.
And forming holes (9) and (10) at least one of which is not located on the center line of the well (6) in the collars (7) and (8) at both ends of the well (6), and 7) and (8) have projections (11) and (12) at the bottom, and two ribs (13), (14) and (15) on both sides of the container.
(16) are integrally molded, and the frame (2) includes horizontal crosspieces (17), (18) continuous vertically and horizontally,
A rectangular frame is formed by the vertical bars (19) and (20), and the opposing horizontal bars (17) and (18) are slightly curved inward with each other.
Grooves (21) and (22) provided at intervals, and pins (25) and (26) projecting from the convex surfaces (23) and (24) located between the grooves, and the horizontal rail (17). , (18) each having a plurality of facing surfaces (27), (28) integrally formed in an arc shape, and the projections (11), (12) of the container (1) are formed in the groove (2) of the frame (2).
Insert holes (9) and (10) into pins (25) and (2) in (1) and (22).
6) respectively, and at the same time, the cylinders (5) at both ends of the well (6) are loosely fitted along the arc shape and the ribs (1)
3), (14), (15) and (16) are facing surfaces (27) and (28)
A device for measuring and detecting a reaction of a substance to be tested, which is formed by combining a plurality of containers (1) in parallel with a frame (2) by engaging with the frame.
【請求項2】容器(1)がポリスチレン製で、フレーム
(2)がアクリルブタジエンスチレン(ABS)製からな
る請求項1記載の被検体物質の反応測定検出器具。
2. The device for measuring and detecting a reaction of a substance to be tested according to claim 1, wherein the container (1) is made of polystyrene and the frame (2) is made of acrylic butadiene styrene (ABS).
JP1990095859U 1990-09-11 1990-09-11 Instrument for measuring and measuring reaction of analyte Expired - Fee Related JPH0734375Y2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP1990095859U JPH0734375Y2 (en) 1990-09-11 1990-09-11 Instrument for measuring and measuring reaction of analyte

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP1990095859U JPH0734375Y2 (en) 1990-09-11 1990-09-11 Instrument for measuring and measuring reaction of analyte

Publications (2)

Publication Number Publication Date
JPH0453555U JPH0453555U (en) 1992-05-07
JPH0734375Y2 true JPH0734375Y2 (en) 1995-08-02

Family

ID=31834931

Family Applications (1)

Application Number Title Priority Date Filing Date
JP1990095859U Expired - Fee Related JPH0734375Y2 (en) 1990-09-11 1990-09-11 Instrument for measuring and measuring reaction of analyte

Country Status (1)

Country Link
JP (1) JPH0734375Y2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011058869A (en) * 2009-09-08 2011-03-24 Tosoh Corp Measuring container and analyzer using the same

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
EP2402089A1 (en) 2003-07-31 2012-01-04 Handylab, Inc. Processing particle-containing samples
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
JP4494167B2 (en) * 2004-11-09 2010-06-30 村角工業株式会社 Sample inspection plate
JP4680037B2 (en) * 2005-11-14 2011-05-11 株式会社エンプラス Fluid handling device and fluid handling unit used therefor
JP5415253B2 (en) 2006-03-24 2014-02-12 ハンディラブ・インコーポレーテッド Integrated system for processing microfluidic samples and methods of use thereof
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
WO2008061165A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic cartridge and method of making same
WO2008060604A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
CA2693654C (en) 2007-07-13 2018-02-13 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8133671B2 (en) 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
JP2009294141A (en) * 2008-06-06 2009-12-17 Enplas Corp Microwell plate
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
JP2011007752A (en) * 2009-06-29 2011-01-13 Beckman Coulter Inc Container set for blood transfusion examination
CA3082652A1 (en) 2011-04-15 2012-10-18 Becton, Dickinson And Company Scanning real-time microfluidic thermocycler and methods for synchronized thermocycling and scanning optical detection
DK2761305T3 (en) 2011-09-30 2017-11-20 Becton Dickinson Co United Reagent Strip
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
EP2773892B1 (en) 2011-11-04 2020-10-07 Handylab, Inc. Polynucleotide sample preparation device
RU2658773C2 (en) 2012-02-03 2018-06-22 Бектон, Дикинсон Энд Компани System and method of implementation of automated assays on plurality of biological samples
USD814653S1 (en) 2014-08-07 2018-04-03 Becton, Dickinson And Company Sample tube holder and components thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0432080Y2 (en) * 1988-09-22 1992-07-31

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011058869A (en) * 2009-09-08 2011-03-24 Tosoh Corp Measuring container and analyzer using the same

Also Published As

Publication number Publication date
JPH0453555U (en) 1992-05-07

Similar Documents

Publication Publication Date Title
JPH0734375Y2 (en) Instrument for measuring and measuring reaction of analyte
US6303389B1 (en) Rapid flow-through binding assay apparatus and method therefor
EP0030086B1 (en) Test-tube assembly, kit for making it and method of manual immunoassay
US5188968A (en) Method and reaction kit for agglutination detection
Shekarchi et al. Evaluation of various plastic microtiter plates with measles, toxoplasma, and gamma globulin antigens in enzyme-linked immunosorbent assays
AU770231B2 (en) Analytical test device and method of use
JP5164971B2 (en) A method for differential detection of multimers from monomers of multimer-forming polypeptides using three-dimensional interactions
Bernard et al. Determination of beta 2-microglobulin in human urine and serum by latex immunoassay.
US4828801A (en) Device for detecting on a nitrocellulose sheet the presence of macromolecular complexes, such as antigens/antibodies
US4400353A (en) Electro-optical system for use in evaluating immunological reactions
JPH0121907B2 (en)
IE44204L (en) Reagent for pregnancy testing
US4628036A (en) Immunological assay with test tube having bottom forming a strip
WO1987003218A1 (en) A disposable microtiter stick
JPH11248706A (en) Non-specific adsorption inhibitor
JPH05504828A (en) Capillary flow device and dual capture assay method
EP0222781A1 (en) Microtiter - surface - flocculation assay for antigen or antibody screening
EP0352139A2 (en) Detecting antigens or antibodies
Zola et al. Diagnostic Immunopathology: Laboratory Practice and Clinical Application
EP0435245A2 (en) Reaction kit
Truant et al. Comparison of an enzyme-linked immunosorbent assay with indirect hemagglutination and hemagglutination inhibition for determination of rubella virus antibody: evaluation of immune status with commercial reagents in a clinical laboratory
JPH0422867A (en) Immune analysis system
AU653965B2 (en) A washing solution, which contains a complexing agent for metal ions, for a solid-phase immunometric method, and the use thereof
Ferraro et al. Comparison of a new, rapid enzyme immunoassay with a latex agglutination test for qualitative detection of rubella antibodies
JPH03191864A (en) Method and apparatus for measuring indirect agglutination immunity

Legal Events

Date Code Title Description
LAPS Cancellation because of no payment of annual fees