JPH07277990A - Bifid bacteria multiplication-stimulating agent - Google Patents
Bifid bacteria multiplication-stimulating agentInfo
- Publication number
- JPH07277990A JPH07277990A JP6088067A JP8806794A JPH07277990A JP H07277990 A JPH07277990 A JP H07277990A JP 6088067 A JP6088067 A JP 6088067A JP 8806794 A JP8806794 A JP 8806794A JP H07277990 A JPH07277990 A JP H07277990A
- Authority
- JP
- Japan
- Prior art keywords
- lactobionic acid
- stimulating agent
- acid
- lactose
- multiplication
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000894006 Bacteria Species 0.000 title abstract description 7
- 239000002269 analeptic agent Substances 0.000 title abstract 5
- UOQHWNPVNXSDDO-UHFFFAOYSA-N 3-bromoimidazo[1,2-a]pyridine-6-carbonitrile Chemical compound C1=CC(C#N)=CN2C(Br)=CN=C21 UOQHWNPVNXSDDO-UHFFFAOYSA-N 0.000 claims abstract description 25
- 229940099563 lactobionic acid Drugs 0.000 claims abstract description 25
- 239000004480 active ingredient Substances 0.000 claims abstract description 7
- 241000186000 Bifidobacterium Species 0.000 claims description 26
- 239000007952 growth promoter Substances 0.000 claims description 7
- 230000000694 effects Effects 0.000 abstract description 9
- RGHNJXZEOKUKBD-SQOUGZDYSA-N D-gluconic acid Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 abstract description 8
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 abstract description 8
- 229950006191 gluconic acid Drugs 0.000 abstract description 8
- 239000008101 lactose Substances 0.000 abstract description 7
- 235000013305 food Nutrition 0.000 abstract description 6
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 abstract description 4
- 239000003814 drug Substances 0.000 abstract description 4
- 229930182830 galactose Natural products 0.000 abstract description 4
- 239000000174 gluconic acid Substances 0.000 abstract description 4
- 235000012208 gluconic acid Nutrition 0.000 abstract description 4
- 239000001963 growth medium Substances 0.000 abstract description 3
- 244000005700 microbiome Species 0.000 abstract description 3
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 abstract description 2
- 101710088194 Dehydrogenase Proteins 0.000 abstract description 2
- 241000589516 Pseudomonas Species 0.000 abstract description 2
- 230000037396 body weight Effects 0.000 abstract description 2
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 abstract description 2
- 229910052794 bromium Inorganic materials 0.000 abstract description 2
- 229940079593 drug Drugs 0.000 abstract description 2
- 244000144972 livestock Species 0.000 abstract description 2
- 230000001590 oxidative effect Effects 0.000 abstract description 2
- 239000000758 substrate Substances 0.000 abstract description 2
- 239000013066 combination product Substances 0.000 abstract 1
- 229940127555 combination product Drugs 0.000 abstract 1
- 239000000126 substance Substances 0.000 description 11
- 230000001737 promoting effect Effects 0.000 description 8
- 239000000203 mixture Substances 0.000 description 7
- 239000002609 medium Substances 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 150000001720 carbohydrates Chemical class 0.000 description 4
- -1 fructose oligosaccharides Chemical class 0.000 description 4
- 229920001542 oligosaccharide Polymers 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 235000015203 fruit juice Nutrition 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 description 2
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 241000606125 Bacteroides Species 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 229920002261 Corn starch Polymers 0.000 description 2
- 229930091371 Fructose Natural products 0.000 description 2
- 239000005715 Fructose Substances 0.000 description 2
- 101000993347 Gallus gallus Ciliary neurotrophic factor Proteins 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 238000013329 compounding Methods 0.000 description 2
- 239000008120 corn starch Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- JCQLYHFGKNRPGE-FCVZTGTOSA-N lactulose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 JCQLYHFGKNRPGE-FCVZTGTOSA-N 0.000 description 2
- 229960000511 lactulose Drugs 0.000 description 2
- PFCRQPBOOFTZGQ-UHFFFAOYSA-N lactulose keto form Natural products OCC(=O)C(O)C(C(O)CO)OC1OC(CO)C(O)C(O)C1O PFCRQPBOOFTZGQ-UHFFFAOYSA-N 0.000 description 2
- 229940099596 manganese sulfate Drugs 0.000 description 2
- 239000011702 manganese sulphate Substances 0.000 description 2
- 235000007079 manganese sulphate Nutrition 0.000 description 2
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 239000007668 pye medium Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 108010050327 trypticase-soy broth Proteins 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- RBCOYOYDYNXAFA-UHFFFAOYSA-L (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl phosphate Chemical compound CC1=NC=C(COP([O-])([O-])=O)C(C)=C1O RBCOYOYDYNXAFA-UHFFFAOYSA-L 0.000 description 1
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 description 1
- 241000193403 Clostridium Species 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- QIJRTFXNRTXDIP-JIZZDEOASA-N L-cysteine hydrochloride hydrate Chemical compound O.Cl.SC[C@H](N)C(O)=O QIJRTFXNRTXDIP-JIZZDEOASA-N 0.000 description 1
- 101100513612 Microdochium nivale MnCO gene Proteins 0.000 description 1
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 1
- 235000019482 Palm oil Nutrition 0.000 description 1
- 101710137908 Pepsin-2 Proteins 0.000 description 1
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 description 1
- 229960001231 choline Drugs 0.000 description 1
- FDJOLVPMNUYSCM-UVKKECPRSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2,7, Chemical compound [Co+3].N#[C-].C1([C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)[N-]\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O FDJOLVPMNUYSCM-UVKKECPRSA-L 0.000 description 1
- 239000002285 corn oil Substances 0.000 description 1
- 235000005687 corn oil Nutrition 0.000 description 1
- 238000005238 degreasing Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 150000002016 disaccharides Chemical class 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 229940036630 folic acid 0.2 mg Drugs 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- BJHIKXHVCXFQLS-UYFOZJQFSA-N fructose group Chemical group OCC(=O)[C@@H](O)[C@H](O)[C@H](O)CO BJHIKXHVCXFQLS-UYFOZJQFSA-N 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 229960001031 glucose Drugs 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 235000021552 granulated sugar Nutrition 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- XBDUTCVQJHJTQZ-UHFFFAOYSA-L iron(2+) sulfate monohydrate Chemical compound O.[Fe+2].[O-]S([O-])(=O)=O XBDUTCVQJHJTQZ-UHFFFAOYSA-L 0.000 description 1
- 239000000905 isomalt Substances 0.000 description 1
- 235000010439 isomalt Nutrition 0.000 description 1
- HPIGCVXMBGOWTF-UHFFFAOYSA-N isomaltol Natural products CC(=O)C=1OC=CC=1O HPIGCVXMBGOWTF-UHFFFAOYSA-N 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 1
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 239000002540 palm oil Substances 0.000 description 1
- 229940055726 pantothenic acid Drugs 0.000 description 1
- 235000019161 pantothenic acid Nutrition 0.000 description 1
- 239000011713 pantothenic acid Substances 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 235000008476 powdered milk Nutrition 0.000 description 1
- 108010009004 proteose-peptone Proteins 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 125000000185 sucrose group Chemical group 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- QYSXJUFSXHHAJI-YRZJJWOYSA-N vitamin D3 Chemical compound C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C\C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-YRZJJWOYSA-N 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、ガラクトースとグルコ
ン酸が結合したラクトビオン酸を有効成分とするビフィ
ズス菌増殖促進剤に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a bifidobacteria growth promoter containing lactobionic acid in which galactose and gluconic acid are combined as an active ingredient.
【０００２】[0002]
【従来の技術】近年、生体内の有用菌であるビフィズス
菌の増殖促進効果を有する物質についての研究が数多く
なされており、その物質として各種オリゴ糖を中心に糖
類が広く注目されている。このビフィズス菌の増殖促進
効果を有する物質としては、従来よりラクチュロースが
良く知られており、既に食品などに添加することにより
その効果が利用されている。しかしながら、ラクチュロ
ースはビフィズス菌のみに選択的に利用される物質でな
い。また、ビフィズス菌の増殖促進効果を有する物質と
して、シュークロースにフラクトースが結合したフラク
トオリゴ糖、ラクトースにガラクトースが結合したガラ
クトオリゴ糖、グルコシド結合でグルコースが連なった
イソマルトオリゴ糖などが知られており、さらに、ビフ
ィズス菌のみが特異的に資化する糖類の開発が求められ
ている現状にある。2. Description of the Related Art In recent years, many studies have been carried out on substances having a growth promoting effect on bifidobacteria, which are useful bacteria in the living body, and saccharides, mainly various oligosaccharides, have been widely noticed as the substances. As a substance having the effect of promoting the growth of Bifidobacteria, lactulose has long been well known, and its effect has already been utilized by adding it to foods and the like. However, lactulose is not a substance that is selectively utilized only by Bifidobacterium. Further, as a substance having a growth promoting effect on Bifidobacterium, fructose oligosaccharides in which sucrose is bound to fructose, galactose oligosaccharides in which galactose is bound to lactose, isomalt oligosaccharides in which glucose is linked by a glucoside bond, and the like are known. In the present situation, it is required to develop a saccharide that is specifically assimilated by Bifidobacterium.
【０００３】一方、既知の物質としてＯ−β−Ｄ−ガラ
クトピラノシル−（１−４）−Ｄ−グルコン酸の一般式
で表される二糖類のラクトビオン酸が知られているが、
このラクトビオン酸がビフィズス菌の増殖促進効果を有
するということは知られていなかった。On the other hand, lactobionic acid, which is a disaccharide represented by the general formula of O-β-D-galactopyranosyl- (1-4) -D-gluconic acid, is known as a known substance.
It has not been known that this lactobionic acid has a growth promoting effect on Bifidobacterium.
【０００４】[0004]
【発明が解決しようとする課題】本発明者らは、上述し
たようにビフィズス菌の増殖促進活性を有する物質を求
めて、鋭意研究を重ねた結果、一般式がＯ−β−Ｄ−ガ
ラクトピラノシル−（１−４）−Ｄ−グルコン酸で表さ
れるラクトビオン酸が特異的にビフィズス菌の増殖促進
活性を有することを見出し、本発明を完成するに至っ
た。したがって、本発明は、ラクトビオン酸を有効成分
とするビフィズス菌増殖促進剤を提供することを課題と
する。DISCLOSURE OF THE INVENTION The inventors of the present invention have conducted extensive studies in search of a substance having a growth-promoting activity for bifidobacteria as described above, and as a result, have found that the general formula is O-β-D-galactopyra. The present inventors have found that lactobionic acid represented by nosyl- (1-4) -D-gluconic acid specifically has a growth promoting activity of bifidobacteria, and completed the present invention. Therefore, it is an object of the present invention to provide a bifidobacteria growth promoter containing lactobionic acid as an active ingredient.
【０００５】[0005]
【課題を解決するための手段】本発明では、一般式がＯ
−β−Ｄ−ガラクトピラノシル−（１−４）−Ｄ−グル
コン酸で表されるラクトビオン酸をビフィズス菌増殖促
進剤の有効成分として用いる。このラクトビオン酸は、
乳糖を基質としてラクトースデヒドロゲナーゼ活性を有
するシュードモナス・グラヴェオレンスなどの微生物を
作用させることにより得ることができ、また、乳糖を臭
素などで酸化することにより得ることもできる。In the present invention, the general formula is O
Lactobionic acid represented by -β-D-galactopyranosyl- (1-4) -D-gluconic acid is used as an active ingredient of the bifidobacteria growth promoter. This lactobionic acid is
It can be obtained by allowing a microorganism such as Pseudomonas graveolens having lactose dehydrogenase activity to act on lactose as a substrate, or by oxidizing lactose with bromine or the like.
【０００６】このビフィズス菌増殖促進効果を有するラ
クトビオン酸は、通常のビフィズス菌を培養する培地に
0.1〜10重量％程度添加して培養すれば良く、また、ヒ
トや家畜の生体内で効果を発揮させるために、飲食品、
医薬品あるいは飼料として、0.05〜2g／kg体重／日程度
摂取させれば良い。そうすれば、ビフィズス菌の増殖を
促進することができる。以下に実施例を示し、本発明を
具体的に説明する。This lactobionic acid, which has the effect of promoting the growth of Bifidobacteria, is used as a medium for culturing ordinary Bifidobacteria.
It may be added in an amount of about 0.1 to 10% by weight and cultured, and in order to exert the effect in humans and livestock, the food and drink,
As a drug or feed, 0.05 to 2 g / kg body weight / day may be taken. Then, the growth of Bifidobacterium can be promoted. Hereinafter, the present invention will be specifically described with reference to examples.
【０００７】[0007]
【実施例１】常法に従い、表１の配合比によって、ビフ
ィズス菌の増殖促進効果を賦与した果汁飲料を製造し
た。Example 1 According to a conventional method, a fruit juice beverage having a bifidobacteria growth-promoting effect was produced at the compounding ratio shown in Table 1.
【０００８】[0008]
【表１】 （単位：重量％） ──────────────────────────────────── 混合異性化糖 15.0 果汁 10.0 クエン酸 1.0 ラクトビオン酸（和光純薬社製） 0.1 香料 0.1 水 73.8 ────────────────────────────────────[Table 1] (Unit:% by weight) ──────────────────────────────────── Mixed isomerized sugar 15.0 Fruit juice 10.0 Citric acid 1.0 Lactobionic acid (manufactured by Wako Pure Chemical Industries, Ltd.) 0.1 Perfume 0.1 Water 73.8 ─────────────────────────────── ──────
【０００９】[0009]
【実施例２】常法に従い、表２の配合比によって、ビフ
ィズス菌の増殖促進効果を賦与したゼリーを製造した。Example 2 According to a conventional method, a jelly having a growth promoting effect on bifidobacteria was produced according to the compounding ratio shown in Table 2.
【００１０】[0010]
【表２】 （単位：重量％） ──────────────────────────────────── 果汁 20.0 グラニュー糖 15.0 水飴 5.0 寒天 1.0 ラクトビオン酸（和光純薬社製） 0.5 香料 0.1 水 58.4 ────────────────────────────────────[Table 2] (Unit:% by weight) ──────────────────────────────────── Fruit juice 20.0 Granulated sugar 15.0 starch syrup 5.0 agar 1.0 lactobionic acid (manufactured by Wako Pure Chemical Industries, Ltd.) 0.5 fragrance 0.1 water 58.4 ──────────────────────────────── ─────
【００１１】[0011]
【実施例３】本発明のラクトビオン酸を配合したビフィ
ズス菌増殖促進剤を調製した。カルシウム2,200g、コー
ンスターチ1,232g、結晶セルロース100g、カルボキシメ
チルセルロースカルシウム 68g及びラクトビオン酸 (和
光純薬社製) 400gをニーダーで混合した後、水 500mlを
噴霧滴下しながら混練した。次に、この混練物を20メッ
シュのスクリーンをセットした単軸オシレーターで造粒
し、流動槽型乾燥機で乾燥した。そして、この乾燥品を
フラッシュミルで粉砕し、整粒して打錠用粉体を得た。
この打錠用粉体に滑沢剤としてショ糖脂肪酸エステル 8
0gをＶ型混合機で混合した後、直径11mmの杵をセットし
た錠剤機で打錠し、平均重量 0.35gのタブレットを得
た。Example 3 A bifidobacteria growth promoter containing the lactobionic acid of the present invention was prepared. 2,200 g of calcium, 1,232 g of corn starch, 100 g of crystalline cellulose, 68 g of calcium carboxymethyl cellulose and 400 g of lactobionic acid (manufactured by Wako Pure Chemical Industries, Ltd.) were mixed with a kneader, and then kneaded while spray-dripping 500 ml of water. Next, this kneaded product was granulated with a uniaxial oscillator having a 20-mesh screen set, and dried with a fluidized-bed dryer. Then, this dried product was crushed by a flash mill and sized to obtain a tableting powder.
Sucrose fatty acid ester was added as a lubricant to this tableting powder.
After mixing 0 g with a V-type mixer, the mixture was tabletted with a tablet machine having a punch having a diameter of 11 mm to obtain tablets having an average weight of 0.35 g.
【００１２】[0012]
【実施例４】常法に従い、表３の配合比によって、ビフ
ィズス菌の増殖促進効果を賦与した犬飼育用飼料（ドッ
グフード）を製造した。Example 4 A feed for dog breeding (dog food) having a growth promoting effect on bifidobacteria was produced according to the mixing ratio shown in Table 3 according to a conventional method.
【００１３】[0013]
【表３】 （単位：重量％） ──────────────────────────────────── 大豆粕 13.7 脱脂粉乳 14.0 大豆油 4.0 コーン油 2.0 パーム油 2.0 トウモロコシ澱粉 23.0 小麦粉 15.0 ふすま 8.0 ビタミン混合物 2.0 ミネラル混合物 9.0 セルロース 2.3 ラクトビオン酸（和光純薬社製） 0.5 ────────────────────────────────────[Table 3] (Unit:% by weight) ──────────────────────────────────── Soybean meal 13.7 Degreasing Powdered milk 14.0 Soybean oil 4.0 Corn oil 2.0 Palm oil 2.0 Corn starch 23.0 Wheat flour 15.0 Bran 8.0 Vitamin mixture 2.0 Mineral mixture 9.0 Cellulose 2.3 Lactobionic acid (Wako Pure Chemical Industries) 0.5 ─────────────── ──────────────────────
【００１４】なお、ビタミン混合物の組成を表４に、ミ
ネラル混合物の組成を表５にそれぞれ示す。The composition of the vitamin mixture is shown in Table 4, and the composition of the mineral mixture is shown in Table 5.
【００１５】[0015]
【表４】 ──────────────────────────────────── ビタミンＡ 1,500 IU ビタミンＤ3 300 IU ビタミンＥ 6.8 mg ビタミンＢ1 0.9 mg ビタミンＢ2 0.4 mg ビタミンＢ6 0.5 mg ビタミンＢ12 3.4 mg ビタミンＣ 50.0 mg パントテン酸 4.0 mg 葉酸 0.2 mg コリン 200.0 mg ビオチン 24.4 μg イノシトール 50.0 mg ナイアシン 10.5 mg ──────────────────────────────────── ショ糖を加えて全量を2gとした。[Table 4] ──────────────────────────────────── Vitamin A 1,500 IU Vitamin D 3 300 IU Vitamin E 6.8 mg Vitamin B 1 0.9 mg Vitamin B 2 0.4 mg Vitamin B 6 0.5 mg Vitamin B 12 3.4 mg Vitamin C 50.0 mg Pantothenic acid 4.0 mg Folic acid 0.2 mg Choline 200.0 mg Biotin 24.4 μg Inositol 50.0 mg Niacin 10.5 mg ──── ──────────────────────────────── Sucrose was added to make the total amount 2 g.
【００１６】[0016]
【表５】 ──────────────────────────────────── ＣａＣＯ3 3.0 g ＫＨ2 ＰＯ4 2.0 g ＮａＨ2 ＰＯ4 1.5 g ＭｇＯ 0.5 g ＭｎＣＯ3 40.0 mg ＦｅＣ6 Ｈ5 Ｏ7 30.0 mg 70％ＺｎＯ 10.0 mg 55％ＣａＣＯ3 4.5 mg ＫｌＯ3 0.65 mg Ｎａ2 ＳｅＯ3 ・５Ｈ2 Ｏ 0.05 mg ＣｒＫ（ＳＯ4 ）・12Ｈ2 Ｏ 5.0 mg ──────────────────────────────────── ショ糖を加えて全量を9gとした。[Table 5] ──────────────────────────────────── CaCO 3 3.0 g KH 2 PO 4 2.0 g NaH 2 PO 4 1.5 g MgO 0.5 g MnCO 3 40.0 mg FeC 6 H 5 O 7 30.0 mg 70% ZnO 10.0 mg 55% CaCO 3 4.5 mg KlO 3 0.65 mg Na 2 SeO 3 / 5H 2 O 0.05 mg CrK (SO 4 ) ・ 12H 2 O 5.0 mg ───────────────────────────────────── It was 9g.
【００１７】[0017]
【試験例】本発明のラクトビオン酸を有効成分とするビ
フィズス菌増殖促進剤について、腸内菌叢を構成する各
種菌類による資化性を確認した。なお、資化性試験に用
いた培地は次の通りである。バクテロイデス属に属する
４菌株については、ＰＹＥ培地（Trypticase 10g、Yeas
t Extract 5g、生理食塩水30ml、馬血液10ml、Pepsin 2
00mg、塩化カルシウム28mg、硫酸マンガン48mg、リン酸
二水素カリウム 440mg、炭酸水素ナトリウム 400mg、塩
化ナトリウム80mg、 L-Cystein-HCl水和物 500mg、精製
水 1,000ml）を用い、それ以外の菌株については、ＬＢ
培地 (Bacto-Liver浸出液 1,000ml、Proteose Peptone
10g、Trypticase 5g 、Yeast Extract 3g、Tween80 1
g、硫酸マグネシウム７水和物 200mg、硫酸第一鉄水和
物10mg、塩化ナトリウム10mg、硫酸マンガン 7mg、L-Cy
stein-HCl 水和物 200mg）を用いた。[Test Example] With respect to the bifidobacteria growth-promoting agent of the present invention containing lactobionic acid as an active ingredient, the assimilation by various fungi forming the intestinal flora was confirmed. The medium used for the assimilation test is as follows. For four strains belonging to the genus Bacteroides, PYE medium (Trypticase 10 g, Yeas
t Extract 5g, physiological saline 30ml, horse blood 10ml, Pepsin 2
00 mg, calcium chloride 28 mg, manganese sulfate 48 mg, potassium dihydrogen phosphate 440 mg, sodium hydrogen carbonate 400 mg, sodium chloride 80 mg, L-Cystein-HCl hydrate 500 mg, purified water 1,000 ml), and other strains , LB
Medium (Bacto-Liver leachate 1,000 ml, Proteose Peptone
10g, Trypticase 5g, Yeast Extract 3g, Tween80 1
g, magnesium sulfate heptahydrate 200 mg, ferrous sulfate hydrate 10 mg, sodium chloride 10 mg, manganese sulfate 7 mg, L-Cy
Stein-HCl hydrate (200 mg) was used.
【００１８】資化性試験は、糖類を添加しないＰＹＥ培
地及びＬＢ培地を基本培地として、これらの培地に無菌
濾過したラクトビオン酸、グルコン酸及びグルコースを
0.5％（Ｗ／Ｖ）になるよう添加した後、供試菌を接種
して37℃で24時間嫌気的に培養した。なお、糖類の資化
性については、培養後の培地の濁度を測定し、 660nmの
吸光度が 0.2以下を（−）、0.21〜 0.5を（±）、0.51
〜 0.9を（＋）、0.91〜 1.4を（＋＋）、1.41以上を
（＋＋＋）として判定した。その結果を表６に示す。The assimilation test was carried out by using aseptic filtered lactobionic acid, gluconic acid, and glucose in PYE medium and LB medium without addition of saccharides as basic medium.
After adding 0.5% (W / V), the test bacteria were inoculated and cultured anaerobically at 37 ° C. for 24 hours. Regarding the assimilation ability of sugars, the turbidity of the culture medium was measured, and the absorbance at 660 nm was 0.2 or less (-), 0.21 to 0.5 (±), 0.51
.About.0.9 was determined as (+), 0.91 to 1.4 as (++), and 1.41 or more as (++++). The results are shown in Table 6.
【００１９】[0019]
【表６】[Table 6]
【００２０】以上のように、ラクトビオン酸は他の糖類
に比べてビフィズス菌に対する高い資化選択性を示し
た。すなわち、バクテロイデスやクロストリジウムなど
の悪玉菌と呼ばれる微生物はラクトビオン酸を全くある
いは殆ど資化しないが、ビフィズス菌はラクトビオン酸
を良く資化することが判った。また、グルコン酸やグル
コースに比べてラクトビオン酸がビフィズス菌に対する
高い選択資化性を示すことが判った。As described above, lactobionic acid showed high assimilation selectivity for Bifidobacteria as compared with other saccharides. That is, it was found that the microorganisms called bad bacteria such as Bacteroides and Clostridium do not utilize lactobionic acid at all or hardly, but Bifidobacteria assimilate lactobionic acid well. In addition, it was found that lactobionic acid showed higher selective assimilation ability against Bifidobacterium than gluconic acid and glucose.
【００２１】[0021]
【発明の効果】ラクトビオン酸はビフィズス菌に対して
高い選択資化性を有するので、ビフィズス菌を培養する
に際して培地に添加する増殖促進剤として有用である。
また、飲食品、医薬品あるいは飼料にビフィズス菌の増
殖促進効果を賦与する増殖促進剤としても有用である。EFFECTS OF THE INVENTION Lactobionic acid has a high selective assimilation activity against Bifidobacteria, and is therefore useful as a growth promoter added to a medium when culturing Bifidobacteria.
Further, it is also useful as a growth promoter for imparting a growth promoting effect of Bifidobacterium to foods and drinks, pharmaceuticals or feeds.
Claims (1)
ズス菌増殖促進剤。1. A bifidobacteria growth promoter comprising lactobionic acid as an active ingredient.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP08806794A JP3559063B2 (en) | 1994-03-31 | 1994-03-31 | Bifidobacterium growth promoter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP08806794A JP3559063B2 (en) | 1994-03-31 | 1994-03-31 | Bifidobacterium growth promoter |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH07277990A true JPH07277990A (en) | 1995-10-24 |
| JP3559063B2 JP3559063B2 (en) | 2004-08-25 |
Family
ID=13932516
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP08806794A Expired - Lifetime JP3559063B2 (en) | 1994-03-31 | 1994-03-31 | Bifidobacterium growth promoter |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3559063B2 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004026840A (en) * | 2003-08-01 | 2004-01-29 | Nitto Yakuhin Kogyo Kk | Viable bacterium composition |
| EP1731042A1 (en) * | 2004-02-06 | 2006-12-13 | Unitika, Ltd. | Feed additive for laying hens and feed containing the additive |
| JP2009143883A (en) * | 2007-12-18 | 2009-07-02 | San-Ei Sucrochemical Co Ltd | Use of composition containing maltobionic acid, its salt, or maltobiono delta lactone |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007116972A (en) * | 2005-10-27 | 2007-05-17 | Unitika Ltd | Bone fracture preventive agent, and slipped tendon preventive agent |
| JP5122766B2 (en) * | 2006-06-20 | 2013-01-16 | ユニチカ株式会社 | Ecole production promoting composition |
-
1994
- 1994-03-31 JP JP08806794A patent/JP3559063B2/en not_active Expired - Lifetime
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004026840A (en) * | 2003-08-01 | 2004-01-29 | Nitto Yakuhin Kogyo Kk | Viable bacterium composition |
| EP1731042A1 (en) * | 2004-02-06 | 2006-12-13 | Unitika, Ltd. | Feed additive for laying hens and feed containing the additive |
| EP1731042A4 (en) * | 2004-02-06 | 2009-07-22 | Unitika Ltd | Feed additive for laying hens and feed containing the additive |
| US8808728B2 (en) | 2004-02-06 | 2014-08-19 | Unitika Ltd. | Feed additive for laying hen and feed containing the same |
| JP2015002748A (en) * | 2004-02-06 | 2015-01-08 | ユニチカ株式会社 | Feed additive for laying hen and feed containing the same |
| JP2009143883A (en) * | 2007-12-18 | 2009-07-02 | San-Ei Sucrochemical Co Ltd | Use of composition containing maltobionic acid, its salt, or maltobiono delta lactone |
Also Published As
| Publication number | Publication date |
|---|---|
| JP3559063B2 (en) | 2004-08-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP1513942B1 (en) | Galactosyl isomalt, method for production and use thereof | |
| CA2467695C (en) | Isomaltooligosaccharides from leuconostoc as neutraceuticals | |
| CN1663573B (en) | A stable and safe microecological formulation, its preparation and usage | |
| WO2019112054A1 (en) | Novel bifidobacterium bacteria and composition including novel bifidobacterium bacteria | |
| US11066684B2 (en) | Production of bioactive oligosaccharides | |
| JP2008504038A (en) | Pre-biological preparation | |
| CN101560523B (en) | Nutrient composition based on mixed fermented culture of five probiotics | |
| KR20060028426A (en) | Composition for lowering serum uric acid level | |
| JP2006502103A (en) | Condensed palatinose and method for producing the same | |
| EP0242459B1 (en) | Use of oligosaccharides for promoting the proliferation of bifidobacteria | |
| JP2002522393A (en) | Method for increasing the solubility of nutrient materials using symbiotic lactic acid producing bacteria | |
| WO2007138905A1 (en) | Intestinal eosinophil-suppressing composition | |
| WO2019112053A1 (en) | Novel bifidobacterium bacteria and composition including novel bifidobacterium bacteria | |
| JP6301024B2 (en) | Felicaribacterium spp. | |
| JPH07277991A (en) | Mineral absorption-stimulating agent | |
| JPH07277990A (en) | Bifid bacteria multiplication-stimulating agent | |
| JP2019097544A (en) | Novel bacteria belonging to bifidobacterium and compositions comprising the same | |
| JP2001231591A (en) | Method of producing mannose and/or mannose oligosaccharide | |
| KR20160143100A (en) | Method for preparing solid culture using bacillus subtilis mori | |
| KR100401350B1 (en) | Non-reducing saccharides, their preparations and uses | |
| WO2002055073A1 (en) | Preventives for arthritis | |
| EP0835658B1 (en) | Enhancing and stabilizing agent of the activity of bifidus factor | |
| KR102284112B1 (en) | A composition for lowering blood glucose level or blood pressure or for use of antioxidant comprising Rhynchosia nulubilis fermented by a yeast and a method for preparing the composition | |
| JP2596509B2 (en) | Novel glucuronic acid-containing oligosaccharide, production method thereof and use thereof | |
| JP2003104880A (en) | Antimutagenic agent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20040427 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20040520 |
|
| R150 | Certificate of patent or registration of utility model |
Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20090528 Year of fee payment: 5 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20100528 Year of fee payment: 6 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20110528 Year of fee payment: 7 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120528 Year of fee payment: 8 |
|
| S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313111 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120528 Year of fee payment: 8 |
|
| R371 | Transfer withdrawn |
Free format text: JAPANESE INTERMEDIATE CODE: R371 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120528 Year of fee payment: 8 |
|
| S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313111 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120528 Year of fee payment: 8 |
|
| R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120528 Year of fee payment: 8 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130528 Year of fee payment: 9 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130528 Year of fee payment: 9 |
|
| EXPY | Cancellation because of completion of term |