JPH07228544A - Water soluble polysaccharide powder and its production - Google Patents

Abstract

PURPOSE:To obtain a water soluble polysaccharide powder safely, simply and reliably, substantially not containing any microbe, thus capable of applying effectively to foods or toiletry materials incapable of being added with any preservative agent and sterilized by heating. CONSTITUTION:This water soluble polysaccharide powder not substantially containing general viable bacilli, thermophilic bacilli, yeasts and fungi is produced by using a process consisting of extracting the water soluble polysaccharide from materials containing the water soluble polysaccharide, and dehydrating and drying the extract after filtrating it. It is produced by adding an oxidation type microbicide in any one step of the process from the extraction to drying, or by contacting with the oxidation type microbicide under a condition where the water soluble polysaccharide is not substantially dissolved after dispersing the water soluble polysaccharide in a poor solvent in which the water soluble polysaccharide does not dissolve, and by conducting a heat treatment.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a water-soluble polysaccharide powder to be added to foods, toiletries and the like, and a method for producing the same, more specifically, by sterilizing with an oxidative germicide. The present invention relates to a water-soluble polysaccharide powder containing substantially no fungus and a method for producing the same.

[0002]

2. Description of the Related Art Dessert frozen desserts such as jelly, pudding, ice cream, yogurt, processed meat products such as ham and sausage, processed fish products such as bamboo rings, kamaboko and surimi,
Sauces, seasonings such as dressings, carbonated drinks, lactic acid bacteria drinks, foods such as beverages such as palatability drinks, toothpaste,
Water-soluble polysaccharides such as carrageenan are widely used as gelling agents, thickeners, emulsifiers, stabilizers, etc. in daily life related products such as fragrances, shampoos, hair dressings, toiletries such as cosmetics. .

On the other hand, such foods, toiletries and the like are subjected to sterilization and bacteriostatic treatment at the time of production in order to prevent spoilage. Generally, after mixing the various ingredients,
Alternatively, in the process of performing various processing, a method of sterilizing by applying heat, a method of adding benzoic acid, sorbic acid, dehydroacetic acid or the like or a salt thereof as a preservative and the like are performed. However, some foods and toiletries
There are some things that can not be sterilized by heating, some things that can not add a preservative from the viewpoint of taste etc.In such a case, it is necessary to keep aseptic from the stage of raw material, gelation As a water-soluble polysaccharide powder used as an agent, a thickener, an emulsifier, a stabilizer, etc., a sterilized and sterilized powder has been required.

[0004]

Conventionally, sterilization of water-soluble polysaccharide powder has hardly been carried out, and generally, general live bacteria and thermophilic bacteria were contained at about 100 to 1000 CFU / g, respectively. As a sterilizing method of such a water-soluble polysaccharide powder, a method using a gas such as ethylene oxide which is used as a normal powder sterilizing method, a method using radiation, a method using ultraviolet rays, a method by heating, Although a method of using high pressure is conceivable, the method of using a gas such as ethylene oxide or radiation is limited in its use from the viewpoint of safety and cannot be used for all powders. In addition, in the method using ultraviolet rays,
Although a sterilizing effect is produced by the irradiation of ultraviolet rays, it is difficult to completely sterilize the powder because it is difficult to uniformly irradiate the entire surface with ultraviolet rays. Further, in the method utilizing high pressure, the equipment is remarkably enlarged, the processing capacity is small, and the productivity is poor.

For this reason, as the optimum sterilization treatment of the water-soluble polysaccharide powder, a method by heating can be mentioned, but generally, sterilization by moist heat is preferable to sterilization by dry heat from the viewpoint of the bactericidal effect. It is a thing. However, in the case of the water-soluble polysaccharide powder, there is a problem that the sterilization by wet heat causes the water-soluble polysaccharide powder to be dissolved, which makes it difficult to handle as a powder. Then, the objective of this invention is providing the manufacturing method which can sterilize the water-soluble polysaccharide powder which does not contain substantially fungi safely, simply, and reliably.

[0006]

In view of such circumstances, the inventors of the present invention have arrived at the present invention as a result of extensive studies on a method for sterilizing a water-soluble polysaccharide powder. That is, the water-soluble polysaccharide powder according to the first aspect of the present invention is characterized in that it does not substantially contain fungi such as general live bacteria, thermophilic bacteria, yeasts and molds. Further, the method for producing a water-soluble polysaccharide powder according to the second aspect of the present invention is to extract the water-soluble polysaccharide from the water-soluble polysaccharide-containing material,
In the manufacturing process of the water-soluble polysaccharide powder in which the extract is filtered, the filtrate is dehydrated and dried, an oxidizing bactericide is added at any stage from extraction to drying. Furthermore, in the method for producing a water-soluble polysaccharide powder according to the third aspect of the present invention, the water-soluble polysaccharide powder is dispersed in a poor solvent in which the water-soluble polysaccharide powder is insoluble, and It is characterized in that the water-soluble polysaccharide powder is heat-treated by being brought into contact with an oxidative germicide in a state where the powder is not dissolved.

In the present invention, the water-soluble polysaccharides include agar, alginic acid and its salts, seaweed polysaccharides extracted from seaweeds such as carrageenan, seed polysaccharides extracted from seeds such as guar gum and locust bean gum, Arabic. Examples thereof include resin exudates such as gum, karaya gum and tragacanth gum, plant extracts extracted from plants such as arabinogalactan and pectin, fermented gums such as xanthan gum and starch.

These water-soluble polysaccharide powders can be extracted by ordinary extraction,
It can be produced by a method such as purification. For example,
In the case of carrageenan, Eucuma Spinosam,
It is produced, for example, by the following production method using red algae such as Eucuma kotoni and Gigartina stellata as a raw material. First, the raw material containing a water-soluble polysaccharide is washed with water, and if necessary, an inorganic or organic acid such as hydrochloric acid, sulfuric acid, citric acid, malic acid or tartaric acid, sodium hydroxide, potassium hydroxide, calcium hydroxide or the like. After pre-treatment with alkali, the sample is immersed in neutral or weakly alkaline water having a pH of about 7 to 10 and heated at a temperature of about 50 to 100 ° C. for about 1 to 8 hours for extraction. Then
It is obtained by adding a filter aid such as diatomaceous earth to the extract and filtering it, and then dehydrating and drying the filtrate by a method such as a gel press method, a drum drying method or an alcohol precipitation method. Further, if necessary, the obtained water-soluble polysaccharide powder can be finely pulverized by a pulverizing method such as an impact pulverizing method, a shearing method and a friction method.

The water-soluble polysaccharide powder of the present invention comprises general live bacteria,
It is essentially free of thermophilic bacteria, yeasts and fungi such as molds. In the present invention, fungi means Staphylococcus and P that grow in a medium temperature range of about 35 ° C.
seudomonas, Streptococcus, B
General live bacteria such as acillus, and B. cereus and B. A thermostable bacterium that is a spore of B. subtilis, which grows in a high temperature region of about 55 ° C. ste
A. thermophilus, C.I. thermos
accharolyticum, C.I. thermoac
S. thermophilic spores of spore-forming bacteria such as eticum, S
Penicil, a yeast of the genus accharomyces
Examples thereof include molds of the genus Lium and the genus Aspergillus. In addition, in the thermophilic bacterium, B. stearothermophilu
spores of spore-forming bacteria such as C. s., which grow under aerobic and thermophilic anaerobic high temperatures. thermosaccharolyt
C. which produces spores of spore-forming bacteria such as icum and anaerobic thermophilic bacteria, which grow under anaerobic high temperature to produce hydrogen sulfate. thermo
Spores of spore-forming bacteria such as aceticum are referred to as sulfidation black bacterium.

In the present invention, the phrase "substantially free of the above fungi" means that no fungi are detected when the obtained water-soluble polysaccharide powder is cultured according to the following culture method. . As for general live cells, 1 g of the water-soluble polysaccharide powder was diluted 100 times with physiological saline, and 2 ml was collected and cultured at 36 ° C. for 48 hours using a standard agar medium. The thermostable bacterium was prepared by diluting 1 g of the water-soluble polysaccharide powder 100 times with physiological saline and heating the diluted solution in a boiling water bath for 10 minutes, and then collecting 2 ml of the diluted solution at 36 ° C. using a standard agar medium. The cells were cultured for 48 hours. For aerobic thermophilic bacteria, 1 g of water-soluble polysaccharide powder is diluted 100 times with physiological saline and heated in a boiling water bath for 10 minutes, then 2 ml is sampled and 5 g of bactotryptone and glucose are added. 3 g and 8 g of agar were dissolved in 1 liter of water and cultured at 55 ° C. for 48 hours. For anaerobic thermophilic bacteria, 1 g of water-soluble polysaccharide powder was diluted 100 times with physiological saline and heated in a boiling water bath for 10 minutes, then 2 ml was collected and used as a medium for measuring Clostridia. And anaerobically cultured at 55 ° C for 7 days. Within the cultured colonies, white ones were anaerobic thermophilic bacteria, and black ones were sulfur black bacterium. Mold,
For yeast, 1 g of water-soluble polysaccharide powder was
After diluting to 00 times, 2 ml was collected and cultured at 25 ° C. for 7 days using potato dexulose agar medium.

The water-soluble polysaccharide powder of the present invention is produced by subjecting the water-soluble polysaccharide to sterilization treatment with an oxidizing bactericide in the process of producing the water-soluble polysaccharide powder. Examples of the oxidative bactericide used in the sterilization treatment include hydrogen peroxide, sodium hypochlorite, and coconut powder. These oxidizing germicides are preferably added in the range of 50 to 5000 ppm with respect to the water-soluble polysaccharide powder. This is because when the amount of the oxidizing bactericide added is less than 50 ppm, a sufficient bactericidal effect tends not to be obtained, and conversely,
This is because if it exceeds 5,000 ppm, the water-soluble polysaccharide itself tends to undergo oxidative decomposition and the physical properties tend to deteriorate.

The sterilization treatment of the water-soluble polysaccharide with the oxidizing bactericidal agent may be carried out during the steps of extraction and purification of the water-soluble polysaccharide, or the water-soluble polysaccharide obtained by a method such as extraction and purification. It may be applied to powder. When performing sterilization treatment during the process of extraction, purification, etc. of the water-soluble polysaccharide, the oxidation-based bactericide is added at any arbitrary stage from the extraction of the water-soluble polysaccharide-containing raw material to the drying. It is carried out by adding in. For example, a method of adding an oxidizing germicide in the extraction step of extracting the water-soluble polysaccharide from the raw material containing the water-soluble polysaccharide, a method of adding an oxidizing germicide in the filtering step of filtering the extract, and a filtrate. Examples include a method of adding an oxidizing bactericidal agent in the dehydration step of dehydration. The addition of the oxidizing disinfectant in these steps may be performed before or after each step, or the required amount of the oxidizing disinfectant may be divided and added before and after each step. In addition, the required amount of the oxidizing germicide may be divided and added in two or more steps. Sterilization of water-soluble polysaccharides is achieved by heating the water-soluble polysaccharides in contact with the oxidative bactericidal agent, but the oxidative bactericidal agent is added to the system during the process as described above. In this case, since heating is performed in the step of adding the oxidative germicide or in the subsequent step, the water-soluble polysaccharide is substantially heat-treated in a state where the water-soluble polysaccharide is in contact with the oxidant germicide. Sterilization of sugar will be performed. In the present invention, it is preferable to add an oxidizing bactericidal agent in the dehydrating step of dehydrating the filtrate. For example, when the dehydrating step is performed by an alcohol precipitation method, the oxidizing bactericidal agent and It may be added, may be added before or after the addition of the alcohol, or may be added after the precipitation of the water-soluble polysaccharide occurs.

When sterilizing the water-soluble polysaccharide powder obtained by the usual methods such as extraction and purification, first,
The water-soluble polysaccharide powder is dispersed in a poor solvent in which it does not dissolve to form a slurry. Examples of the poor solvent in which the water-soluble polysaccharide powder is insoluble include, for example, alcohols such as methanol, ethanol, and propanol, acetone, ketones such as methyl ethyl ketone, and the like, in consideration of use in foods, ethanol or Propanol is preferred. In these poor solvents, a range in which the water-soluble polysaccharide powder does not dissolve,
For example, water may be added in the range of 1 to 50% by weight to form an aqueous solution.

The mixing ratio of the poor solvent and the water-soluble polysaccharide powder is a weight ratio of 0.
It is preferably 5 to 5. This is because if the poor solvent is mixed in excess of 5 times, it takes time to remove or recover the solvent, resulting in poor productivity. Conversely, if it is less than 0.5 times, it is difficult to form a uniform slurry. This is because the sterilization of the water-soluble polysaccharide powder tends to be uneven and the sterilization cannot be performed uniformly.

Next, in a state where the water-soluble polysaccharide powder is not substantially dissolved, the water-soluble polysaccharide powder is dispersed in a poor solvent to form a slurry, and an oxidizing bactericidal agent is added to the powder to oxidize the powder. After the addition of the system bactericide, heating is performed for sterilization. Heating
It is carried out by a method of raising the temperature of the entire slurry system to which an oxidizing germicide is added. At this time, the heating temperature is preferably in the range of 50 to 95 ° C. This is because if the temperature is lower than 50 ° C, sterilization takes a long time and productivity tends to be poor. On the contrary, if the temperature exceeds 95 ° C, the quality of the water-soluble polysaccharide powder tends to deteriorate. The heating time is 30 minutes to 5 minutes.
It is preferably about time. This is a heating time of 3
This is because if it is less than 0 minutes, a sufficient bactericidal effect tends not to be obtained, and if it exceeds 5 hours, the quality of the water-soluble polysaccharide powder tends to be deteriorated. It is desirable that the heat treatment be performed as uniformly as possible, and it is preferable that the heat treatment be performed while stirring or the like.

After the heat treatment, heating is continued as it is to dry, or reduced pressure is applied to dry the poor solvent to remove the water-soluble polysaccharide powder. The water-soluble polysaccharide powder of the present invention thus obtained is substantially free of fungi such as general live bacteria, thermophilic bacteria, yeasts and molds.

[0017]

EXAMPLES The present invention will be described below with reference to examples. Example 1 Staphylococcus and Ps were used as general viable bacteria.
eudomonas, Streptococcus, etc. 1
Locust bean gum powder containing 000 CFU / g 20g
40 ml of 60% aqueous ethanol and 800 ppm of sodium hypochlorite (against locust bean gum powder)
It was added and kept at a temperature of 60 ° C. for 5 hours. Then, it was dried under reduced pressure and the sterilized locust bee gum powder was taken out. The sterilized locust bean gum powder obtained,
The cells were cultured according to the above-mentioned culture method, but no viable bacteria were detected.

Example 2 As a mesophilic thermostable bacterium, 20 g of carrageenan powder containing 100 CFU / g of spores of the genus Bacillus was added to 80 g.
% Aqueous solution of isopropyl alcohol and 300 ppm of hydrogen peroxide (against carrageenan powder) were added, and the mixture was kept at a temperature of 95 ° C. for 1 hour. Then, it was dried under reduced pressure and the sterilized carrageenan powder was taken out. The sterilized carrageenan powder obtained was cultured according to the above-mentioned culture method, but thermostable bacteria were not detected.

Example 3 To 20 g of xanthan gum powder containing 100 CFU / g of aerobic thermophilic bacterium, 80 ml of 70% aqueous ethanol solution and 1000 ppm of sodium hypochlorite (against xanthan gum powder) were added, and the mixture was heated at 80 ° C. for 3 days. Held for hours. Then, it was dried under reduced pressure and the sterilized xanthan gum powder was taken out. The sterilized xanthan gum powder obtained was cultivated according to the culturing method described above, but no aerobic thermophilic bacterium was detected.

Example 4 20 g of arabinogalactan powder containing 10 CFU / g of sulfur black bacterium, 100 ml of 90% ethanol aqueous solution and 3000 ppm of hydrogen peroxide (against arabinogalactan powder)
It was added and kept at a temperature of 55 ° C. for 4 hours. Then, it was dried under reduced pressure and the sterilized arabinogalactan powder was taken out. The sterilized arabinogalactan powder obtained was cultured according to the above-mentioned culture method, but no sulfur black bacterium was detected.

Example 5 20 g of pectin powder containing 5000 CFU / g of mold of the genus Asergillus was added to an aqueous solution of 85% ethanol 3
0 ml and hydrogen peroxide 3000 ppm (against pectin powder)
It was added and held at a temperature of 70 ° C. for 4 hours. Then, it was dried under reduced pressure and the sterilized pectin powder was taken out.
The obtained sterilized pectin powder was cultured according to the above-mentioned culture method, but no mold was detected.

Example 6 2000 CFU / g of general bacteria and 2 of mesophilic thermostable bacteria
20 g of guar gum powder containing 00 CFU / g, 40 ml of 80% aqueous ethanol solution and 100 parts of sodium hypochlorite
Add 0ppm (vs guar gum powder) and add 2 at 90 ℃
Held for hours. Then, it dried under reduced pressure and took out the sterilized guar gum powder. The obtained sterilized guar gum powder was cultured according to the above-mentioned culture method, but general viable bacteria and thermostable bacteria were not detected.

Example 7 As a mesophilic thermostable bacterium, 6 kg of a raw material alga containing carrageenan containing spores of the genus Bacillus was washed with water, and then 10
Immerse in 0 liter of water and adjust with potassium hydroxide to pH 9.1.
And heated to 80 ° C. to extract carrageenan. 1 kg of diatomaceous earth was added to the obtained extract and filtration was performed.
0 liter of filtrate was obtained. To 1 part by weight of the obtained filtrate, 1 part by weight of isopropanol and 300 pp of hydrogen peroxide
m (vs carrageenan) was added to precipitate the carrageenan. The obtained carrageenan precipitate was dried at 80 ° C. for 30 minutes to obtain a carrageenan powder. The sterilized carrageenan powder obtained was cultured according to the above-mentioned culture method, but thermostable bacteria were not detected.

[0024]

INDUSTRIAL APPLICABILITY According to the present invention, a water-soluble polysaccharide powder containing substantially no fungus can be produced safely, simply and surely, and a preservative cannot be added or is heated by heating. It can be effectively applied to foods and toiletry products that cannot be sterilized.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Hirofumi Tsuji 141-2 Kitagomitsuka, Mie-gun Kusu-cho, Mie Prefecture (72) Inventor Yuuji Kato 1-1-18 Shinsho, Yokkaichi-shi, Mie Prefecture

Claims (3)

[Claims] 1. A water-soluble polysaccharide powder which is substantially free of fungi such as general live bacteria, thermophilic bacteria, yeasts and molds. 2. A process for producing a water-soluble polysaccharide powder, which comprises extracting a water-soluble polysaccharide from a substance containing a water-soluble polysaccharide, filtering the extract, and then dehydrating and drying the filtrate. A method for producing a water-soluble polysaccharide powder, which comprises adding an oxidative germicide at an arbitrary stage. 3. A water-soluble polysaccharide powder is dispersed in a poor solvent in which the water-soluble polysaccharide powder is insoluble, and oxidation-based sterilization is performed under a state in which the water-soluble polysaccharide powder is not substantially dissolved. A method for producing a water-soluble polysaccharide powder, which comprises subjecting the composition to contact with an agent for heat treatment.