JPH07184657A - C4 photosynthesis-related gene in rice plant and its promoter - Google Patents

Abstract

PURPOSE:To obtain a new cDNA useful for preparing a new variant of a plant by gene recombination and modifying a cultured cell for producing a metbolise. CONSTITUTION:This cDNA of pyruvate phosphate dikinase gene of rice plant has a base sequence encoding 947 amino acid sequence of the formula. An RNA is extracted from a rice plant at about three weeks after the germination, its cDNA is synthesized, the cDNA is linked to a phage vector and a cDNA library is prepared. A sequence specific to PPDK gene is used as a probe and a cDNA of PPDK is obtained from the cDNA library.

Description

Detailed Description of the Invention

[0001]

The present invention relates to rice (Oryza Sativa
L.) pyruvate orthophosphate dikinase (PPDK) protein gene and its promoter, which are used for the development of new plant varieties using gene recombination and the modification of cultured cells for producing metabolites. obtain.

[0002]

2. Description of the Related Art Development of a transformation technique for imparting a new trait to a plant by introducing a foreign gene is desired. Hitherto, as a transformation promoter, cauliflower mosaic virus (Ca, which efficiently expresses a gene in plant cells) has been used.
The promoter of 35S RNA of MV) has been mainly used. However, this promoter is not expressed specifically in the plant growth period or period, and may be inconvenient depending on the properties of the target gene. Therefore, it is expected to develop promoters having various properties such that they are expressed specifically in the growing season and / or organs depending on the purpose.

Generally, plants can be classified into C3 plants that perform C3 photosynthesis (rice, wheat, etc.) and C4 plants that perform C4 photosynthesis (corn, sugarcane, etc.) depending on the difference in the CO ₂ metabolic pathway of photosynthesis. C4 photosynthesis is C3
It is a photosynthetic system with a CO ₂ concentration mechanism added, and has a CO ₂ fixing capacity 1.5 to 2 times that of C3 photosynthesis. It is the PPDK enzyme that is responsible for the most important regulation of this mechanism. In other words, C4 plant PPDK (C4 type PPDK)
Is the most important enzyme in the family of enzymes involved in C4 photosynthesis. The C4 type PPDK gene has two promoters and produces PPDK localized in chloroplasts, roots and stems of green leaf tissues, respectively. In recent years, PP showing a similar expression form to C4 type PPDK in C3 plants
The existence of DK was revealed. Furthermore, it was found that the C3 type PPDK corresponding to the PPDK expressed in the roots and stems of C4 plants is highly expressed in spikelets (the part where the fruits are produced) in C3 plants such as rice and wheat. .

[0004]

[Means for Solving the Problems] By utilizing two kinds of promoters of the PPDK gene of C3 plants, the present inventors have investigated the growth time in chloroplasts of green leaf tissue, spikelets, roots, stems and the like. And / or it may be possible to express a useful gene in an organ-specific manner.
By utilizing the structural portion of the PPDK gene of the C3 plant, it is linked to a promoter that actively expresses in rice, and this is returned to rice or other plants, so that expression similar to that of the C4 plant is performed. Since it is possible to impart a C4 photosynthetic function with excellent fixing efficiency, and as a result, it is possible to increase the photosynthetic ability of C3 plants such as rice by 1.5 to 2 times, and from rice of C3 plants, PPDK is used. The gene and its two promoters were isolated.

Since the transcripts transcribed from these two promoters have different lengths, the organ-specific expression state of each promoter was examined by Northern hybridization analysis. As a result, the promoter producing a long transcript (promoter 1) has an expression function in green leaves, and the promoter producing a short transcript (promoter 2) has a particularly strong expression ability in spikelets,
It was revealed that the roots and stems also showed some expression.

That is, the DNA fragment of the present invention is a cDNA of the rice PPDK gene having a nucleotide sequence encoding the amino acid sequence represented by SEQ ID NO: 25 in the late sequence listing,
In particular, it includes cDNAs having the nucleotide sequences shown in SEQ ID NOs: 2, 3 and 6-24 in the sequence listing. Further, the DN of the present invention
The A fragment includes the cDNA of the rice PPDK gene having the nucleotide sequence encoding the amino acid sequence represented by SEQ ID NO: 26 in the sequence listing, particularly the cDNA having the nucleotide sequence represented by SEQ ID NOS: 5-24 in the sequence listing. Furthermore, D of the present invention
The NA fragment is a promoter 1 expressed in leaf blades of rice having the nucleotide sequence represented by SEQ ID NO: 1 in the sequence listing and a promoter 2 expressed in spikelets of rice having the nucleotide sequence represented by SEQ ID NO: 4 in the sequence listing. including.

Further, in the DNA fragment of the present invention, a part of the bases or amino acids of the base sequences or amino acid sequences shown in SEQ ID NOs: 1-28 are replaced with other bases or amino acids, as long as the function is not substantially impaired. May be
Alternatively, it may be deleted, or a part of the base sequence or the amino acid sequence may be transposed. All of these derivatives are included in the DNA fragment of the present invention.

The present invention will be described in detail below. The PPDK gene of the present invention can be isolated as follows. First, rice that has been germinated for about 3 weeks is prepared. Using this as a starting material, RNA was extracted by a conventional method, and its cDNA was extracted.
To synthesize. This cDNA is connected to a phage vector to prepare a cDNA library. Using a sequence specific to the PPDK gene as a probe, the cDNA of PPDK is isolated from the cDNA library.

Next, genomic DNA is extracted from rice grown in the dark for about 2 weeks and partially digested with a restriction enzyme to obtain a DNA fragment having a size of about 20 Kbp. This is connected to a phage vector to create a genomic library.

The sequence on the 5'side of the above PPDK cDNA and 3
The rice PPDK gene and its two promoters are isolated from the genomic library using the sequence on the side of the side as a probe.

FIG. 1 shows a schematic diagram of the rice PPDK gene. The two arrows indicate the transcription initiation points of the transcripts expressed in the leaf blades and spikelets, respectively, and the promoter regions corresponding to the transcription initiation points are present upstream thereof. The intron (intervening sequence) 2 existing between these two promoters is
It is a very large intron of 10 Kbp or more, and the entire nucleotide sequence of the genome excluding this is shown in SEQ ID NOs: 27 and 28 of the sequence listing.

In the sequences shown in SEQ ID NOS: 27 and 28, two promoters and 21 exons each correspond to the sequences shown in each SEQ ID NO: as follows: SEQ ID NO: 1 promoter 1 2 exon 1 3 Exon 2 4 Promoter 2 5 Exon 3 (first half) 6 Exon 3 (second half) 7 Exon 4 8 Exon 5 9 Exon 6 10 Exon 7 11 Exon 8 12 Exon 9 13 Exon 10 14 Exon 11 15 Exon 12 16 Exon 13 17 Exon 14 18 Exon 15 19 Exon 16 20 Exon 17 21 Exon 18 22 Exon 19 23 Exon 20 24 Exon 21

The sequences shown in SEQ ID NO: 1 and SEQ ID NO: 4 are a promoter region expressed in leaf blade (promoter 1) and a promoter region expressed in spikelets (promoter 2), respectively. Leaf blade PPDK and spikelet P
Regarding PDK, the base number 1 of SEQ ID NOS: 2 and 5 indicates the transcription start point, and the ATG of base numbers 46 to 48 of SEQ ID NO: 3 and the base numbers 69 to 71 of SEQ ID NO: 5 indicate the translation start point, respectively.

More specifically, the structure of each PPDK gene will be described.
2 exons (SEQ ID NOs: 2, 3, and 6-24)
Exon 1 consists of 0 introns and encodes a transit peptide required for PPDK protein to enter the chloroplast (SEQ ID NO: 3, bases 46 to 2).
58, amino acid sequences 1 to 71). In transcription of this gene, exon 3 begins at the 99th base (SEQ ID NO: 6, base number 1). In the promoter region which is the promoter region thereof, TATA box (SEQ ID NO: 1, base numbers 1380 to 1384; the first base of exon 1 is set to +1 and the position is −39 to −35 when counted in the upstream direction), 8 times Repeat sequence (similarly -363 to -14
9) exists. The repeated sequence of 8 times is shown in Table 1.

[0015]

[Table 1]

PPDK expressed in spikelets is composed of 19 exons (SEQ ID NOS: 5 to 24) and 18 introns, and has a nucleotide sequence common to the leaf blade PPDK except for a part of exon 3. In promoter 2 which is the promoter region, TATA box (SEQ ID NO: 4, base numbers 729 to 734; the first base of exon 3 is +1 and counting in the upstream direction is −32 to −27), CAAT box (the same, Base number 639 to 643; -122 to -11
8) exists.

As described above, by detecting two transcripts having different sizes of the PPDK gene (Northern hybridization method), the characteristics of the promoter corresponding to each transcript can be determined.
The expression level of PPDK in each organ of rice is shown in FIG.
As a result, a large number of short transcripts were expressed in spikelets, some expression was observed in roots and stems, and it became clear that promoter 2 is a promoter specifically expressed in spikelets. It was FIG. 3 shows a long transcript expressed in the green leaf of rice, and the spike transcript was shown as a control. As a result, it was revealed that promoter 1 is a promoter expressed in chloroplasts of green leaves, that is, photosynthetic organs.

Hereinafter, the present invention will be described in more detail with reference to examples, but the present invention is not limited to these examples.

[0019]

EXAMPLE The PPDK gene was cloned as follows. First, total RNA was extracted from the seedlings 3 weeks after germination by the guanidine thiocyanate method. From this total RNA, poly A was obtained by oligo dT cellulose column.
⁺ -RNA was isolated. The yield of this poly A ⁺ -RNA was about 1% of the total RNA. The synthesis of cDNA from poly A ⁺ -RNA was prepared using a commercially available synthesis kit (Pharmacia, etc.). The synthesized cDNA
A cDNA library was obtained by connecting to the EcoRI site of λZAP, which is a phage vector, and packaging.

Using the structural portion of the known PPDK gene as a probe, plaque hybridization
A positive clone was obtained from the cDNA library. When analyzed by sequencing or the like, it was determined to be the rice PPDK gene because it has a similar structure to the known PPDK gene obtained from corn. It was found that the PPDK genes of maize and rice have about 85% homology in the translation region alone, but little homology in the promoter part.

Next, a rice genomic library was prepared. After seeding, DNA was extracted from the yellow seedlings of rice grown in the dark for 2 weeks by the CTAB method. DNA 1
0 μg was partially digested with a commercially available restriction enzyme Sau3AI (manufactured by Takara Shuzo Co., Ltd.) and subjected to 10-40% sucrose density gradient centrifugation to obtain a DNA fragment of about 20 Kbp. This was ligated to the BamHI site of the phage vector EMBL3 and packaged to prepare a genomic library.

Using the nucleotide sequence corresponding to exon 1 and the nucleotide sequence corresponding to exon 3 of the cDNA as probes, a genomic library was screened and four positive clones could be obtained. When the nucleotide sequences of the insert DNAs of these clones were determined by the dideoxy method, it was confirmed that the rice PPDK gene and its two promoters could be isolated because they all corresponded to PPDK cDNA. In Figure 1, rice P
A schematic diagram of the PDK gene is shown. In FIG. 1, arrows indicate two transcription start points and boxes indicate translation regions.

Next, Northern hybridization was carried out in order to examine the characteristics of the two promoters.
Total RNA was extracted from sprouts, green leaves, sprouts during greening, leaf sheaths, branch shoots, stems, and yellow sprouts, and about 20 μg of each was used as a sample, and the nucleotide sequences before and after exon 3 were used as probes. As a result, as shown in FIG. 2, it was confirmed that promoter 2 has a function of specifically expressing a large amount in spikelets. Sp: spikelet, Gr: green leaf, G
i: sprout during greening, Ls: leaf sheath, Rb: branch, St:
Stem, Ro: Root, Et: Shows yellow seedlings, numbers are mRN
The size of A (Kbp) is shown. In addition, in order to perform more sensitive Northern hybridization, all RN of green leaves
Poly A ⁺ -using oligo dT cellulose column from A
RNA was isolated, the expression level of PPDK in the green leaves was examined, and its size was clarified by comparison with the PPDK of spikelets. The result is shown in FIG. In FIG. 3, total RNA was used for spikelets and poly A ⁺ -RNA was used for green leaves. Numbers indicate mRNA size (Kbp). This confirmed the expression of promoter 1 in green leaves.

[0024]

EFFECT OF THE INVENTION Promoter 1 Developed by the Present Invention
By constructing a transformant using, it became possible to appropriately express the target gene in green leaves.
Using promoter 2 makes it possible to specifically express a large amount of the gene of interest in spikelets (fruit parts), improve rice varieties, and specifically control fruit parts from pests and diseases. can do. By using the structural portion of the PPDK gene or the cDNA sequence of the present invention, there is a possibility that a C3 plant can be provided with a photosynthetic function such as C4 photosynthesis, which is excellent in carbon dioxide fixation efficiency.

[0025]

[Sequence listing] SEQ ID NO: 1 Sequence length: 1418 Sequence type: Nucleic acid Number of strands: Double-stranded Topology: Linear Sequence type: Genomic DNA Origin organism name: Oryza Sativa L. Sequence characteristics represents symbol: TATA Signal present position: 1380 ... 1384 method to determine the characteristics: E AAGCTTGAAC TGGAGCCATG CCTACCCTCG CGGAGTCATC GGCAAGCCGC ATCATCTTTC 60 TCTCCTTGTC GAGCTCCTCC TACGCAATCT TAGCCTCCCG TATCATCTTC ACCGCCTCGC 120 CACCCCTGCC ATCCATGCCG CCGGTGGCAA CGAGGCCGCT CCTCACAAGC TCGTCGGACA 180 GTTGCTGCGA CCCTACTAGT CCGCCATGAC CGTTGCCCCT CTCCTCGTGG GGTTAGGTCA 240 CTTAGGAGTA TGTCTCTCTC CCTGTCCGGC TTGCAGCATA CACGTATCCG CGCTTTGCTC 300 GCCTGCGTGC ACTCGCCAAG CGATGACTCG CCCTCCCTCG TTGAGGCCAC GGGTGCTAAA 360 GCATATGGTG ACGCCTTCTT CGCACACCTA TGTCACCACC ATTTTCGTCT CCACCTCACC 420 ACTAAGCACA TCCACCTCTG TTGGTTGCAT CGACGTCGCT TCCCTAGCTC CCGTCTCTAG 480 TCCGGATCCT ATTCCTCCTT GGAGACCGAA GCTACCGCAA CCATTGCTCG GTGGTTAGCG 540 AGCGTGGAGC TGTCCTCCCC ACTTTCGCGT CCTCGTTC GC CACCACAGCC ATACTTCGCA 600 TGGTGATGTC TTCTCCTTCA CTCACCGCTA AACTCAGTGC AACCGTTTCT ACCCTAGCCC 660 CGGCCGCCGC TCTCATAGAG GTGAAAGTTC ATTTACATGT AGGTCCCACA TGTTTTATGT 720 TTTTTATTTT TCTTTTACTG ATTAGCATGC CACGTAAATC AAAACAACAA TCCATAGTGT 780 TTTAAGTATT TTTATTTAAT ACGTGAGATG GAGTACAAAA ACGAGAGATG CAAAGTGAAC 840 TTGCTAAAAC ACATTTTCTG GTTGATTACA CTCGCTTGTT GAGCCATTGG ATCGGTCATA 900 GGATTCGTGC TAGCATACTT AATTACGCGT AACTAGTTGT GCTTTATAGG TTACAGGTCG 960 CTAATTAGCG GTCTACTGGA GAACTTTGCT ACTATTTTTT TCTTCACTGC ATGCACTCGA 1020 TCAAGTATGA GTATTTGTAC CGACCAGCGA AACACATATG TAATTAAAGT ATAAATATGT 1080 AATTAGTATA TATTAGTAGT ATATTTAGAC AGTAGTTACA CCCTACATAC ACACCACTTA 1140 CATATATAAT TAGTATGTAA TTTTGTAACT TACATATGTA ATTTTAGTAC TTACATATGT 1200 AATTTTGAGA CTTACATTGT AAATACACTA AAATTACATA TGTAATTTAG TAACCTACAA 1260 TGTAAATACA TGCCGACTAA CTTTTCATGA AAAATATCCC TGTTATAAAT ATAGCTACTC 1320 CCGAACTTTA TTCCTTCTCT GTGAGATATC AGTGGAAACG CTCGGTGGAA TCGGGGGAGT 1380 ATTTGGGAGC ACGCGCCGAC GCGCGCGTCG TGCGTGCC 1418

SEQ ID NO: 2 Sequence length: 351 Sequence type: Nucleic acid Number of strands: Double-stranded topology: Linear Sequence type: cDNA to mRNA Origin organism name: Oryza Sativa L. GTCGTCTTTG TCGCGGTGGA GCGGAGCGCG CCCACTTGCG CGCCTGGGCC GGAGGCGGGC 60 GCGCCGGGGG TTCGGGAATC CCCTGGAGCC ACACGTAAAG GCGCGGGCGG GAGGGAGGGA 120 GGGGCCAGCT AGGATAAGGC ACGCGCGGCC GCTGCGATTG GGGCGCTTGT GAACACCGGG 180 GCGCCACGTG GAGAGGACGT TACACTCCAG CCGCCAAATT TCCACTCCCA CACCCGCGCT 240 CCCCTCCCCT CTCTTTTCCG TGATCGCACC TCGCCCACGC GCCCCCCGCC ACACACAATC 300 TCTGCAGCTC TCCAGCTTCG TTGGAACTCG CGAATCTCTC TCCGATCCCA G 351

SEQ ID NO: 3 Sequence length: 270 Sequence type: Nucleic acid Number of strands: Double strand Topology: Linear Sequence type: cDNA to mRNA Origin organism name: Oryza Sativa L. Sequence characteristics Symbol: transit peptide Location: 46 ... 258 Method for determining characteristics: E GTGAAGCGCG ACTTGGAGTT GGAGGGGAGA TCGATCAGGG CCAGG 45 ATG CCG TCG GTT TCG AGG GCC GTG TGC GTG CAG AGG GCG AGC GGG AAT 93 Met Pro Ser Val Ser Arg Ala Val Cys Val Gln Arg Ala Ser Gly Asn 1 5 10 15 AAC GGC AGG AGG TGC AGA GAT GGG GCG GCC GCC GCC GGC CGC CGA TCC 141 Asn Gly Arg Arg Cys Arg Asp Gly Ala Ala Ala Ala Gly Arg Arg Ser 20 25 30 GTC GTG GCG CAG AGA GCG CGG CAC GGC AAG CCG GAG GTC GCC ATC CGC 189 Val Val Ala Gln Arg Ala Arg His Gly Lys Pro Glu Val Ala Ile Arg 35 40 45 TCG GGG TCC GGC GGC TCG GCG CGG GGC GGG CAT TGC TCG CCT CTC AGG 237 Ser Gly Ser Gly Gly Ser Ala Arg Gly Gly His Cys Ser Pro Leu Arg 50 55 60 GCA GTG GCG GCG CCG ATC CCG ACT ACC AAA AAG 270 Ala Val A la Ala Pro Ile Pro Thr Thr Lys Lys 65 70 75

SEQ ID NO: 4 Sequence length: 760 Sequence type: Nucleic acid Number of strands: Double strand Topology: Linear Sequence type: Genomic DNA Origin organism name: Oryza Sativa L. Sequence characteristics Symbol: CAAT signal Location: 639 ... 644 Characteristic determining method: E Characteristic symbol: TATA signal Location: 729 ... 734 Characteristic determining method: E AAAACTTCGT CGCTTCCCCG AGGCAAATCG GCAACATGCC GGTCGCCAGC GGGTCCAGCG 60 CACCGGTATG CCCGGCACC CTTTTGTAGTT 60AG 120 TGCTGGACAT ACCCTGAGGT TTATCCAGCA ACAAAACGCC GTTAATGTCG CGACCGCGAC 180 GACGAGGACG ACTCATTAGT CCTCCTTGCT GTCGTCCGGG TTAACACGAC GTTCTTCGTC 240 ATGTTTGACC ACGCTGGTCA CCAGGTTTGA CATGCGCATC CCTTCAACCA GAGAGTTGTC 300 GTAGAAGAAG GTCAGTTCCG GCACGATACG CAGGCGCATC GCTTTCCCCA GCAGGCTGCG 360 GATGAAACCA GAAGCTTGCA TTTTATCTAT AACACGTCTA ATTTCTTGTG GGCACTGCAC 420 ATATTCCCCT GGTTTTGATA CAGCGTATCC AAAATTCACT CACACTTAAA AGCTCAAAAA 480 AGCTCCCATT TTAATCACCA CACGTCTAAC AAATTTCTTG TTCACATCCA CAGAAGAAGC 540 TATCCATGCT GTACTTTACA TTGCAGTATT AGACTTTTTA TACTACTTTT ACATTACATT 600 ATTAGACCTT TTTTTAACAC AAAAATCCAC CTACCCAACC AATTTTTTGC CGGGCTGGTC 660 CTCCTCCCCC CGCATGAGCC GCCCGTGCGA TGACGTCTCC CGGTGGGTCA CACCGTCACA 720 CACCGTGCTA TAAATAGGGG GGCTTGGCCT CTCCGCCATG 760

SEQ ID NO: 5 Sequence length: 98 Sequence type: Nucleic acid Number of strands: Double strand Topology: Linear Sequence type: cDNA to mRNA Origin organism name: Oryza Sativa L. AGCACCACAC TTCACCACTT CGCTTTGCAC AAAGCCTCAG TGCCTCACTA CACTTGCACC 60 GATCACTA ATG GCT CCG GCT CAA TGT GCT CGT GTG CAG 98 Met Ala Pro Ala Gln Cys Ala Arg Val Gln 1 5 10

SEQ ID NO: 6 Sequence length: 57 Sequence type: Nucleic acid Number of strands: Double strand Topology: Linear Sequence type: cDNA to mRNA Origin organism name: Oryza Sativa L. AGG GTG TTC CAC TTC GGC AAG GGC AAG AGC GAG GGC AAC AAG GCC ATG 48 Arg Val Phe His Phe Gly Lys Gly Lys Ser Glu Gly Asn Lys Ala Met 76 80 85 90 AAG GAC CTG 57 Lys Asp Leu 94

SEQ ID NO: 7 Sequence length: 234 Sequence type: Nucleic acid Number of strands: Double strand Topology: Linear Sequence type: cDNA to mRNA Origin organism name: Oryza Sativa L. CTG GGA GGG AAG GGG GCG AAC TTG GCG GAG ATG GCG AGC ATC GGG CTG 48 Leu Gly Gly Lys Gly Ala Asn Leu Ala Glu Met Ala Ser Ile Gly Leu 95 100 105 110 TCG GTG CCG CCG GGG TTC ACG GTG TCG ACG GAG GCG TGC CAG CAG TAC 96 Ser Val Pro Pro Gly Phe Thr Val Ser Thr Glu Ala Cys Gln Gln Tyr 115 120 125 CAG GCG GCG GGG AAG ACG CTG CCG GCG GGG CTG TGG GAG GAG ATC GTC 144 Gln Ala Ala Gly Lys Thr Leu Pro Ala Gly Leu Trp Glu Glu Ile Val 130 135 140 GAG GGG CTC CAG TGG GTG GAG GAG TAC ATG GCG GCG CGC CTC GGC GAC 192 Glu Gly Leu Gln Trp Val Glu Glu Tyr Met Ala Ala Arg Leu Gly Asp 145 150 155 CCC GCC CGC CCT CTC CTC CTC TCC GTC CGC TCC GGC GCC GCC 234 Pro Ala Arg Pro Leu Leu Leu Ser Val Arg Ser Gly Ala Ala 160 165 170 172

SEQ ID NO: 8 Sequence length: 138 Sequence type: Nucleic acid Number of strands: Double strand Topology: Linear Sequence type: cDNA to mRNA Origin organism name: Oryza Sativa L. GTG TCG ATG CCG GGG ATG ATG GAC ACG GTG CTG AAC CTG GGG CTG AAC 48 Val Ser Met Pro Gly Met Met Asp Thr Val Leu Asn Leu Gly Leu Asn 173 175 175 185 GAC GAG GTG GCG GCG GGA CTG GCG GCC AAG AGC GGC GAC CGC TTC GCG 96 Asp Glu Val Ala Ala Gly Leu Ala Ala Lys Ser Gly Asp Arg Phe Ala 190 195 200 TAC GAC TCC TAC CGC CGT TTC CTC GAC ATG TTC GGC AAC GTG 138 Tyr Asp Ser Tyr Arg Arg Phe Leu Asp Met Phe Gly Asn Val 205 210 215 218

SEQ ID NO: 9 Sequence length: 163 Sequence type: Nucleic acid Number of strands: Double strand Topology: Linear Sequence type: cDNA to mRNA Origin organism name: Oryza Sativa L. GTC ATG GAC ATT CCT CAT GCA CTG TTC GAG GAG AAG CTC GAA GCC ATG 48 Val Met Asp Ile Pro His Ala Leu Phe Glu Glu Lys Leu Glu Ala Met 219 220 225 230 AAA GCA GTC AAG GGG CTG CAC AAC GAC ACT GAC CTG ACT GCC ACT GAC 96 Lys Ala Val Lys Gly Leu His Asn Asp Thr Asp Leu Thr Ala Thr Asp 235 240 245 250 CTC AAA GAA CTA GTG GCA CAG TAC AAG GAT GTC TAT GTT GAA GCT AAG 144 Leu Lys Glu Leu Val Ala Gln Tyr Lys Asp Val Tyr Val Glu Ala Lys 255 260 265 GGA GAG CCA TTC CCC TCT G 163 Gly Glu Pro Phe Pro Ser 270 272

SEQ ID NO: 10 Sequence length: 236 Sequence type: Nucleic acid Number of strands: Double strand Topology: Linear Sequence type: cDNA to mRNA Origin organism name: Oryza Sativa L. AT CCC AAG AAG CAA CTG CAG CTG GCC GTG TTG GCC GTG TTC AAC TCA 47 Asp Pro Lys Lys Gln Leu Gln Leu Ala Val Leu Ala Val Phe Asn Ser 273 275 280 285 TGG GAT AGC CCA AGA GCA ATC AAG TAC AGA AGC ATA AAC AAG ATC ACT 95 Trp Asp Ser Pro Arg Ala Ile Lys Tyr Arg Ser Ile Asn Lys Ile Thr 290 295 300 GGG CTG AAG GGC ACT GCT GTA AAC GTG CAG ACC ATG GTG TTT GGA AAC 143 Gly Leu Lys Gly Thr Ala Val Asn Val Gln Thr Met Val Phe Gly Asn 305 310 315 320 ATG GGG AAC ACC TCT GGC ACG GGT GTG CTC TTC ACT AGG AAC CCA AGC 191 Met Gly Asn Thr Ser Gly Thr Gly Val Leu Phe Thr Arg Asn Pro Ser 325 330 335 ACT GGA GAG AAG AAG CTT TAC GGC GAA TTC CTT GTG AAT GCT CAG 236 Thr Gly Glu Lys Lys Leu Tyr Gly Glu Phe Leu Val Asn Ala Gln 340 345 350 350 351

SEQ ID NO: 11 Sequence length: 129 Sequence type: Nucleic acid Number of strands: Double strand Topology: Linear Sequence type: cDNA to mRNA Origin organism name: Oryza Sativa L. GGT GAA GAT GTG GTT GCT GGA ATC AGA ACT CCA GAG GAT CTT GAT GCT 48 Gly Glu Asp Val Val Ala Gly Ile Arg Thr Pro Glu Asp Leu Asp Ala 352 355 360 365 ATG AGG GAC CAC ATG CCG GAG CCT TAT GAA GAG CTC GTT GAG AAC TGC 96 Met Arg Asp His Met Pro Glu Pro Tyr Glu Glu Leu Val Glu Asn Cys 370 375 380 AAA ATT TTG GAG AGT CAC TAT AAA GAA ATG ATG 129 Lys Ile Leu Glu Ser His Tyr Lys Glu Met Met 385 390 394

SEQ ID NO: 12 Sequence length: 180 Sequence type: Nucleic acid Number of strands: Double strand Topology: Linear Sequence type: cDNA to mRNA Origin organism name: Oryza Sativa L. GAT ATT GAA TTT ACT GTT CAG GAA AAT AGG CTT TGG ATG CTT CAG TGC 48 Asp Ile Glu Phe Thr Val Gln Glu Asn Arg Leu Trp Met Leu Gln Cys 395 400 405 410 AGA ACA GGA AAG CGC ACA GGA AAA GGT GCT GTA AAG ATT GCT CTA GAC 96 Arg Thr Gly Lys Arg Thr Gly Lys Gly Ala Val Lys Ile Ala Leu Asp 415 420 425 ATG GTT AAC GAG GGC CTT GTT GAG CGC CGC ACA GCA CTT AAG ATG GTA 144 Met Val Asn Glu Gly Leu Val Glu Arg Arg Thr Ala Leu Lys Met Val 430 435 440 GAA CCA GGT CAC CTG GAT CAG CTT CTT CAT CCT CAG 180 Glu Pro Gly His Leu Asp Gln Leu Leu His Pro Gln 445 450 454

SEQ ID NO: 13 Sequence length: 138 Sequence type: Nucleic acid Number of strands: Double strand Topology: Linear Sequence type: cDNA to mRNA Origin organism name: Oryza Sativa L. TTT GAG AAC CCA TCA GGG TAT AAA GAC AAA GTT ATT GCC ACG GGC TTA 48 Phe Glu Asn Pro Ser Gly Tyr Lys Asp Lys Val Ile Ala Thr Gly Leu 455 460 465 470 CCT GCA TCA CCT GGG GCT GCT GTG GGC CAA ATT GTA TTC ACT GCC GAG 96 Pro Ala Ser Pro Gly Ala Ala Val Gly Gln Ile Val Phe Thr Ala Glu 475 480 485 GAT GCT GAA GCA TGG CAT GCC CAA GGG AAA GAT GTT ATT CTG 138 Asp Ala Glu Ala Trp His Ala Gln Gly Lys Asp Val Ile Leu 490 495 500

SEQ ID NO: 14 Sequence length: 165 Sequence type: Nucleic acid Number of strands: Double strand Topology: Linear Sequence type: cDNA to mRNA Origin organism name: Oryza Sativa L. GTG AGG ACA GAG ACC AGC CCA GAG GAT GTT GGT GGC ATG CAT GCA GCT 48 Val Arg Thr Glu Thr Ser Pro Glu Asp Val Gly Gly Met His Ala Ala 501 505 510 515 GTT GGA ATT CTT ACA GCA AGA GGT GGT ATG ACC TCT CAC GCT GCT GTT 96 Val Gly Ile Leu Thr Ala Arg Gly Gly Met Thr Ser His Ala Ala Val 520 525 530 GTT GCG CGC GGA TGG GGC AAA TGT TGT GTG TCA GGA TGC TCA AGC GTC 144 Val Ala Arg Gly Trp Gly Lys Cys Cys Val Ser Gly Cys Ser Ser Val 535 540 545 CGT GTA AAT GAT GCG TCC AAG 165 Arg Val Asn Asp Ala Ser Lys 550 555

SEQ ID NO: 15 Sequence length: 162 Sequence type: Nucleic acid Number of strands: Double strand Topology: Linear Sequence type: cDNA to mRNA Origin organism name: Oryza Sativa L. ATT GTA GTG ATT GAA GAC AAG GCG CTG CAT GAA GGT GAG TGG CTA TCG 48 Ile Val Val Ile Glu Asp Lys Ala Leu His Glu Gly Glu Trp Leu Ser 556 560 565 570 TTG AAT GGA TCA ACT GGT GAA GTG ATC ATT GGC AAG CAG CCA CTC TGC 96 Leu Asn Gly Ser Thr Gly Glu Val Ile Ile Gly Lys Gln Pro Leu Cys 575 580 585 CCA CCA GCC CTT AGT GGT GAT TTG GAA ACT TTC ATG TCT TGG GTG GAT 144 Pro Pro Ala Leu Ser Gly Asp Leu Glu Thr Phe Met Ser Trp Val Asp 590 595 600 GAA GTT AGG AAA CTC AAG 162 Glu Val Arg Lys Leu Lys 605 609

SEQ ID NO: 16 Sequence length: 90 Sequence type: Nucleic acid Number of strands: Double strand Topology: Linear Sequence type: cDNA to mRNA Origin organism name: Oryza Sativa L. GTT ATG GCA AAT GCT GAT ACC CCC GAG GAT GCA ACA ACA GCC AGA CAG 48 Val Met Ala Asn Ala Asp Thr Pro Glu Asp Ala Thr Thr Ala Arg Gln 610 615 620 625 AAT GGG GCA GAA GGA ATT GGG CTA TGC CGG ACT GAA CAT ATG 90 Asn Gly Ala Glu Gly Ile Gly Leu Cys Arg Thr Glu His Met 630 635 639

SEQ ID NO: 17 Sequence length: 139 Sequence type: Nucleic acid Number of strands: Double strand Topology: Linear Sequence type: cDNA to mRNA Origin organism name: Oryza Sativa L. TTC TTC GCT TCA GAC GAG AGG ATT AAG GCT GTC AGG CAG ATG ATT ATG 48 Phe Phe Ala Ser Asp Glu Arg Ile Lys Ala Val Arg Gln Met Ile Met 640 645 650 655 GCT TCA AGT CTT GAA CTG AGG CAG AAA GCA CTA GAT CGC CTT TTG CCT 96 Ala Ser Ser Leu Glu Leu Arg Gln Lys Ala Leu Asp Arg Leu Leu Pro 660 665 670 TAT CAG AGG TCT GAC TTT GAA GGC ATC TTC CGT GCA ATG GAT G 139 Tyr Gln Arg Ser Asp Phe Glu Gly Ile Phe Arg Ala Met Asp 675 680 685

SEQ ID NO: 18 Sequence length: 178 Sequence type: Nucleic acid Number of strands: Double-stranded topology: Linear Sequence type: cDNA to mRNA Origin organism name: Oryza Sativa L. GG CTT CCG GTA ACT ATT AGA CTC TTG GAT CCT CCA CTT CAT GAG TTC 47 Gly Leu Pro Val Thr Ile Arg Leu Leu Asp Pro Pro Leu His Glu Phe 686 690 695 700 CTT CCA GAA GGC CAT GTT GAG GAT ATG GTG CGT GAG CTA TGC TCT GAA 95 Leu Pro Glu Gly His Val Glu Asp Met Val Arg Glu Leu Cys Ser Glu 705 710 715 ACT GGA GCA GCT CAG GAT GAT GTC CTT GCA AGA GTA GAA AAA CTT TCC 143 Thr Gly Ala Ala Gln Asp Asp Val Leu Ala Arg Val Glu Lys Leu Ser 720 725 730 GAA GTA AAT CCA ATG CTT GGT TTC CGT GGG TGC AG 178 Glu Val Asn Pro Met Leu Gly Phe Arg Gly Cys Arg 735 740 745

SEQ ID NO: 19 Sequence length: 130 Sequence type: Nucleic acid Number of strands: Double strand Topology: Linear Sequence type: cDNA to mRNA Origin organism name: Oryza Sativa L. G CTT GGT ATA TCA TAC CCT GAA TTG ACT GAA ATG CAA GCC CGT GCC ATC 49 Leu Gly Ile Ser Tyr Pro Glu Leu Thr Glu Met Gln Ala Arg Ala Ile 746 750 755 760 TTT GAA GCT GCT ATA ACC ATG ACC AAC CAG GGT ATT CAA GTC TTT CCG 97 Phe Glu Ala Ala Ile Thr Met Thr Asn Gln Gly Ile Gln Val Phe Pro 765 770 775 GAG ATA ATG GTT CCC CTT GTT GGA ACT CCT CAG 130 Glu Ile Met Val Pro Leu Val Gly Thr Pro Gln 780 785 788

SEQ ID NO: 20 Sequence length: 129 Sequence type: Nucleic acid Number of strands: Double strand Topology: Linear Sequence type: cDNA to mRNA Origin organism name: Oryza Sativa L. GAA TTG GGG CAT CAA GTG GAT GTT ATC CGT CAA ATT GCC AAC AAG GTG 48 Glu Leu Gly His Gln Val Asp Val Ile Arg Gln Ile Ala Asn Lys Val 789 790 795 800 TTC ACA GAC ATG GGA AAA ACT ATT GGC TAC AAA GTT GGA ACT ATG ATT 96 Phe Thr Asp Met Gly Lys Thr Ile Gly Tyr Lys Val Gly Thr Met Ile 805 810 815 820 GAA ATT CCC AGG GCA GCT TTA GTG GCT GAT GAG 129 Glu Ile Pro Arg Ala Ala Leu Val Ala Asp Glu 825 830 831

SEQ ID NO: 21 Sequence length: 138 Sequence type: Nucleic acid Number of strands: Double strand Topology: Linear Sequence type: cDNA to mRNA Origin organism name: Oryza Sativa L. ATA GCA GAG CAG GCT GAG TTC TTC TCC TTT GGA ACA AAC GAC CTA ACA 48 Ile Ala Glu Gln Ala Glu Phe Phe Ser Phe Gly Thr Asn Asp Leu Thr 832 835 840 845 CAG ATG ACA TTT GGT TAC AGT AGG GAT GAT GTG GGG AAG TTC CTT CCC 96 Gln Met Thr Phe Gly Tyr Ser Arg Asp Asp Val Gly Lys Phe Leu Pro 850 855 860 ATC TAT CTG TCT CAG GGT ATC CTC CAG CAT GAC CCC TTT GAG 138 Ile Tyr Leu Ser Gln Gly Ile Leu Gln His Asp Pro Phe Glu 865 870 875 877

SEQ ID NO: 22 Sequence length: 78 Sequence type: Nucleic acid Number of strands: Double strand Topology: Linear Sequence type: cDNA to mRNA Origin organism name: Oryza Sativa L. GTG CTT GAT CAG AGG GGA GTT GGG GAG CTG GTT AAG CTG GCT ACA GAG 48 Val Leu Asp Gln Arg Gly Val Gly Glu Leu Val Lys Leu Ala Thr Glu 878 880 885 890 AGA GGC CGC AAA GCT AGG CCT AAC TTG AAG 78 Arg Gly Arg Lys Ala Arg Pro Asn Leu Lys 895 900 903

SEQ ID NO: 23 Sequence length: 92 Sequence type: Nucleic acid Number of strands: Double strand Topology: Linear Sequence type: cDNA to mRNA Origin organism name: Oryza Sativa L. GTG GGC ATC TGT GGT GAA CAT GGT GGA
GAG CCT CTG TCA GTC GCT TTC 48 Val Gly Ile Cys Gly Glu His Gly Gly Glu Pro Leu Ser Val Ala Phe 904 905 910 915 TTT GCG AAG GCT GGG CTG GAC TAT GTT TCT TGT TCG CCT TTC AG 92 Phe Ala Lyu Ala Lys Ala Lys Ala Gly Glu Asp Tyr Val Ser Cys Ser Pro Phe Arg 920 925 930 934

SEQ ID NO: 24 Sequence length: 288 Sequence type: Nucleic acid Number of strands: Double strand Topology: Linear Sequence type: cDNA to mRNA Origin organism name: Oryza Sativa L. G GTC CCA ATT GCT AGG CTA GCT GCA GCT CAG GTG CTC CTC TGA TGA 46 Val Pro Ile Ala Arg Leu Ala Ala Ala Gln Val Leu Leu 935 940 945 947 TGA CGC CCT TCT CTT TAC CGG CAA CCA GAT CGC CTG CTG TTG GTG CAC 94 CTG GTG AAG AAT AAT AAA TAC ATA GCC ATG GAA TGG TGG AAA CTC TGC 142 TGC CTG TCA AAG GCG CCG TTG TAT GTA GTA TGC ATG TTC GCA TGT AAG 190 CCT ATG TGG TGG TAG TTT TCT GAA AAT TTC GCT TGA ACT GCC ACG GTA 238 ATG TAC CAA ACA GCG ATG ACG ACG AAG AAT CGA ATA AAG GTG GTG ATT 286 AC 288

SEQ ID NO: 25 Sequence length: 947 Sequence type: Amino acid Sequence type: Peptide Met Pro Ser Val Ser Arg Ala Val Cys Val Gln Arg Ala Ser Gly Asn 1 5 10 15 Asn Gly Arg Arg Cys Arg Asp Gly Ala Ala Ala Ala Gly Arg Arg Ser 20 25 30 Val Val Ala Gln Arg Ala Arg His Gly Lys Pro Glu Val Ala Ile Arg 35 40 45 Ser Gly Ser Gly Gly Ser Ala Arg Gly Gly His Cys Ser Pro Leu Arg 50 55 60 Ala Val Ala Ala Pro Ile Pro Thr Thr Lys Lys Arg Val Phe His Phe 65 70 75 80 Gly Lys Gly Lys Ser Glu Gly Asn Lys Ala Met Lys Asp Leu Leu Gly 85 90 95 Gly Lys Gly Ala Asn Leu Ala Glu Met Ala Ser Ile Gly Leu Ser Val 100 105 110 Pro Pro Gly Phe Thr Val Ser Thr Glu Ala Cys Gln Gln Tyr Gln Ala 115 120 125 Ala Gly Lys Thr Leu Pro Ala Gly Leu Trp Glu Glu Ile Val Glu Gly 130 135 140 Leu Gln Trp Val Glu Glu Tyr Met Ala Ala Arg Leu Gly Asp Pro Ala 145 150 155 160 Arg Pro Leu Leu Leu Ser Val Arg Ser Gly Ala Ala Val Ser Met Pro 165 170 175 Gly Met Met Asp Thr Val Leu Asn Leu Gly Leu Asn Asp Glu Val Ala 180 185 190 Ala Gly Leu Ala Ala Lys Ser Gly Asp Arg Phe Ala Tyr Asp Ser Tyr 195 200 205 Arg Arg Phe Leu Asp Met Phe Gly Asn Val Val Met Asp Ile Pro His 210 215 220 Ala Leu Phe Glu Glu Lys Leu Glu Ala Met Lys Ala Val Lys Gly Leu 225 230 235 240 His Asn Asp Thr Asp Leu Thr Ala Thr Asp Leu Lys Glu Leu Val Ala 245 250 255 Gln Tyr Lys Asp Val Tyr Val Glu Ala Lys Gly Glu Pro Phe Pro Ser 260 265 270 Asp Pro Lys Lys Gln Leu Gln Leu Ala Val Leu Ala Val Phe Asn Ser 275 280 285 Trp Asp Ser Pro Arg Ala Ile Lys Tyr Arg Ser Ile Asn Lys Ile Thr 290 295 300 Gly Leu Lys Gly Thr Ala Val Asn Val Gln Thr Met Val Phe Gly Asn 305 310 315 320 Met Gly Asn Thr Ser Gly Thr Gly Val Leu Phe Thr Arg Asn Pro Ser 325 330 335 Thr Gly Glu Lys Lys Leu Tyr Gly Glu Phe Leu Val Asn Ala Gln Gly 340 345 350 Glu Asp Val Val Ala Gly Ile Arg Thr Pro Glu Asp Leu Asp Ala Met 355 360 365 Arg Asp His Met Pro Glu Pro Tyr Glu Glu Leu Val Glu Asn Cys Lys 370 375 380 Ile Leu Glu Ser His Tyr Lys Glu Met Met Asp Ile Glu Phe Thr Val 385 390 395 400 Gln Glu Asn Arg Leu Trp Met Leu Gln Cys Arg Thr Gly Lys Arg Thr 405 410 415 Gly Lys Gly Ala Val Lys Ile Ala Leu Asp Met Val Asn Glu Gly Leu 420 425 430 Val Glu Arg Arg Thr Ala Leu Lys Met Val Glu Pro Gly His Leu Asp 435 440 445 Gln Leu Leu His Pro Gln Phe Glu Asn Pro Ser Gly Tyr Lys Asp Lys 450 455 460 Val Ile Ala Thr Gly Leu Pro Ala Ser Pro Gly Ala Ala Val Gly Gln 465 470 475 480 Ile Val Phe Thr Ala Glu Asp Ala Glu Ala Trp His Ala Gln Gly Lys 485 490 495 Asp Val Ile Leu Val Arg Thr Glu Thr Ser Pro Glu Asp Val Gly Gly 500 505 510 Met His Ala Ala Val Gly Ile Leu Thr Ala Arg Gly Gly Met Thr Ser 515 520 525 His Ala Ala Val Val Ala Arg Gly Trp Gly Lys Cys Cys Val Ser Gly 530 535 540 Cys Ser Ser Val Arg Val Asn Asp Ala Ser Lys Ile Val Val Ile Glu 545 550 555 560 Asp Lys Ala Leu His Glu Gly Glu Trp Leu Ser Leu Asn Gly Ser Thr 565 570 575 Gly Glu Val Ile Ile Gly Lys Gln Pro Leu Cys Pro Pro Ala Leu Ser 580 585 590 Gly Asp Leu Glu Thr Phe Met Ser Trp Val Asp Glu Val Arg Lys Leu 595 600 605 Lys Val Met Ala Asn Ala Asp Thr Pro Glu Asp Ala Thr Thr Ala Arg 610 615 620 Gln Asn Gly Ala Glu Gly Ile Gly Leu Cys Arg Thr Glu His Met Phe 625 630 635 640 Phe Ala Ser Asp Glu Arg Ile Lys Ala Val Arg Gln Met Ile Met Ala 645 650 655 Ser Ser Leu Glu Leu Arg Gln Lys Ala Leu Asp Arg Leu Leu Pro Tyr 660 665 670 Gln Arg Ser Asp Phe Glu Gly Ile Phe Arg Ala Met Asp Gly Leu Pro 675 680 685 Val Thr Ile Arg Leu Leu Asp Pro Pro Leu His Glu Phe Leu Pro Glu 690 695 700 Gly His Val Glu Asp Met Val Arg Glu Leu Cys Ser Glu Thr Gly Ala 705 710 715 720 Ala Gln Asp Asp Val Leu Ala Arg Val Glu Lys Leu Ser Glu Val Asn 725 730 735 Pro Met Leu Gly Phe Arg Gly Cys Arg Leu Gly Ile Ser Tyr Pro Glu 740 745 750 Leu Thr Glu Met Gln Ala Arg Ala Ile Phe Glu Ala Ala Ile Thr Met 755 760 765 Thr Asn Gln Gly Ile Gln Val Phe Pro Glu Ile Met Val Pro Leu Val 770 775 780 Gly Thr Pro Gln Glu Leu Gly His Gln Val Asp Val Ile Arg Gln Ile 785 790 795 800 Ala Asn Lys Val Phe Thr Asp Met Gly Lys Thr Ile Gl y Tyr Lys Val 805 810 815 Gly Thr Met Ile Glu Ile Pro Arg Ala Ala Leu Val Ala Asp Glu Ile 820 825 830 Ala Glu Gln Ala Glu Phe Phe Ser Phe Gly Thr Asn Asp Leu Thr Gln 835 840 845 Met Thr Phe Gly Tyr Ser Arg Asp Asp Val Gly Lys Phe Leu Pro Ile 850 855 860 Tyr Leu Ser Gln Gly Ile Leu Gln His Asp Pro Phe Glu Val Leu Asp 865 870 875 880 Gln Arg Gly Val Gly Glu Leu Val Lys Leu Ala Thr Glu Arg Gly Arg 885 890 895 Lys Ala Arg Pro Asn Leu Lys Val Gly Ile Cys Gly Glu His Gly Gly 900 905 910 Glu Pro Leu Ser Val Ala Phe Phe Ala Lys Ala Gly Leu Asp Tyr Val 915 920 925 Ser Cys Ser Pro Phe Arg Val Pro Ile Ala Arg Leu Ala Ala Ala Gln 930 935 940 Val Leu Leu 945 947

SEQ ID NO: 26 Sequence length: 882 Sequence type: Amino acid Sequence type: Peptide Met Ala Pro Ala Gln Cys Ala Arg Val Gln Arg Val Phe His Phe Gly 1 5 10 15 Lys Gly Lys Ser Glu Gly Asn Lys Ala Met Lys Asp Leu Leu Gly Gly 20 25 30 Lys Gly Ala Asn Leu Ala Glu Met Ala Ser Ile Gly Leu Ser Val Pro 35 40 45 Pro Gly Phe Thr Val Ser Thr Glu Ala Cys Gln Gln Tyr Gln Ala Ala 50 55 60 Gly Lys Thr Leu Pro Ala Gly Leu Trp Glu Glu Ile Val Glu Gly Leu 65 70 75 80 Gln Trp Val Glu Glu Tyr Met Ala Ala Arg Leu Gly Asp Pro Ala Arg 85 90 95 Pro Leu Leu Leu Ser Val Arg Ser Gly Ala Ala Val Ser Met Pro Gly 100 105 110 Met Met Asp Thr Val Leu Asn Leu Gly Leu Asn Asp Glu Val Ala Ala 115 120 125 Gly Leu Ala Ala Lys Ser Gly Asp Arg Phe Ala Tyr Asp Ser Tyr Arg 130 135 140 Arg Phe Leu Asp Met Phe Gly Asn Val Val Met Asp Ile Pro His Ala 145 150 155 160 Leu Phe Glu Glu Lys Leu Glu Ala Met Lys Ala Val Lys Gly Leu His 165 170 175 Asn Asp Thr Asp Leu Thr Ala Thr Asp Leu Lys Glu Leu Val Ala Gln 180 185 190 Tyr Lys Asp Val Tyr Val Glu Ala Lys Gly Glu Pro Phe Pro Ser Asp 195 200 205 Pro Lys Lys Gln Leu Gln Leu Ala Val Leu Ala Val Phe Asn Ser Trp 210 215 220 Asp Ser Pro Arg Ala Ile Lys Tyr Arg Ser Ile Asn Lys Ile Thr Gly 225 230 235 240 Leu Lys Gly Thr Ala Val Asn Val Gln Thr Met Val Phe Gly Asn Met 245 250 255 Gly Asn Thr Ser Gly Thr Gly Val Leu Phe Thr Arg Asn Pro Ser Thr 260 265 270 Gly Glu Lys Lys Leu Tyr Gly Glu Phe Leu Val Asn Ala Gln Gly Glu 275 280 285 Asp Val Val Ala Gly Ile Arg Thr Pro Glu Asp Leu Asp Ala Met Arg 290 295 300 Asp His Met Pro Glu Pro Tyr Glu Glu Leu Val Glu Asn Cys Lys Ile 305 310 315 320 Leu Glu Ser His Tyr Lys Glu Met Met Asp Ile Glu Phe Thr Val Gln 325 330 335 Glu Asn Arg Leu Trp Met Leu Gln Cys Arg Thr Gly Lys Arg Thr Gly 340 345 350 Lys Gly Ala Val Lys Ile Ala Leu Asp Met Val Asn Glu Gly Leu Val 355 360 365 Glu Arg Arg Thr Ala Leu Lys Met Val Glu Pro Gly His Leu Asp Gln 370 375 380 Leu Leu His Pro Gln Phe Glu Asn Pro Ser Gly Tyr Lys Asp Lys Val 385 390 395 400 Ile Ala Thr Gly Leu Pro Ala Ser Pro Gly Ala Ala Val Gly Gln Ile 405 410 415 Val Phe Thr Ala Glu Asp Ala Glu Ala Trp His Ala Gln Gly Lys Asp 420 425 430 Val Ile Leu Val Arg Thr Glu Thr Ser Pro Glu Asp Val Gly Gly Met 435 440 445 His Ala Ala Val Gly Ile Leu Thr Ala Arg Gly Gly Met Thr Ser His 450 455 460 Ala Ala Val Val Ala Arg Gly Trp Gly Lys Cys Cys Val Ser Gly Cys 465 470 475 480 Ser Ser Val Arg Val Asn Asp Ala Ser Lys Ile Val Val Ile Glu Asp 485 490 495 Lys Ala Leu His Glu Gly Glu Trp Leu Ser Leu Asn Gly Ser Thr Gly 500 505 510 Glu Val Ile Ile Gly Lys Gln Pro Leu Cys Pro Pro Ala Leu Ser Gly 515 520 525 Asp Leu Glu Thr Phe Met Ser Trp Val Asp Glu Val Arg Lys Leu Lys 530 535 540 Val Met Ala Asn Ala Asp Thr Pro Glu Asp Ala Thr Thr Ala Arg Gln 545 550 555 560 Asn Gly Ala Glu Gly Ile Gly Leu Cys Arg Thr Glu His Met Phe Phe 565 570 575 Ala Ser Asp Glu Arg Ile Lys Ala Val Arg Gln Met Ile Met Ala Ser 580 585 590 Ser Leu Glu Leu Arg Gln Lys Ala Leu Asp Arg Leu Le u Pro Tyr Gln 595 600 605 Arg Ser Asp Phe Glu Gly Ile Phe Arg Ala Met Asp Gly Leu Pro Val 610 615 620 Thr Ile Arg Leu Leu Asp Pro Pro Leu His Glu Phe Leu Pro Glu Gly 625 630 635 640 His Val Glu Asp Met Val Arg Glu Leu Cys Ser Glu Thr Gly Ala Ala 645 650 655 Gln Asp Asp Val Leu Ala Arg Val Glu Lys Leu Ser Glu Val Asn Pro 660 665 670 Met Leu Gly Phe Arg Gly Cys Arg Leu Gly Ile Ser Tyr Pro Glu Leu 675 680 685 Thr Glu Met Gln Ala Arg Ala Ile Phe Glu Ala Ala Ile Thr Met Thr 690 695 700 Asn Gln Gly Ile Gln Val Phe Pro Glu Ile Met Val Pro Leu Val Gly 705 710 715 720 Thr Pro Gln Glu Leu Gly His Gln Val Asp Val Ile Arg Gln Ile Ala 725 730 735 Asn Lys Val Phe Thr Asp Met Gly Lys Thr Ile Gly Tyr Lys Val Gly 740 745 750 Thr Met Ile Glu Ile Pro Arg Ala Ala Leu Val Ala Asp Glu Ile Ala 755 760 765 Glu Gln Ala Glu Phe Phe Ser Phe Gly Thr Asn Asp Leu Thr Gln Met 770 775 780 Thr Phe Gly Tyr Ser Arg Asp Asp Val Gly Lys Phe Leu Pro Ile Tyr 785 790 795 800 Leu Ser Gln Gly Ile Leu Gln His Asp Pro Phe Glu Va l Leu Asp Gln 805 810 815 Arg Gly Val Gly Glu Leu Val Lys Leu Ala Thr Glu Arg Gly Arg Lys 820 825 830 Ala Arg Pro Asn Leu Lys Val Gly Ile Cys Gly Glu His Gly Gly Glu 835 840 845 Pro Leu Ser Val Ala Phe Phe Ala Lys Ala Gly Leu Asp Tyr Val Ser 850 855 860 Cys Ser Pro Phe Arg Val Pro Ile Ala Arg Leu Ala Ala Ala Gln Val 865 870 875 880 Leu Leu 882

SEQ ID NO: 27 Sequence length: 2666 Sequence type: Nucleic acid Number of strands: Double stranded Topology: Linear Sequence type: Genomic DNA Origin organism name: Oryza Sativa L. Characteristic of sequence Symbol: promoter Location of existence: 1 ... 1418 Method of determining feature: E Feature symbol: TATA signal Location of presence: 1380 ... 1384 Method of determining feature: E Feature symbol: exon Presence location: 1419 ... 1769 Determined method: E Characteristic symbol: intron Presence position: 1770 ... 1885 Method of determining characteristic: E Characteristic symbol: exon Presence position: 1886 ... 2155 Method of determining characteristic: E Characteristic symbol: transit peptide Location: 1931 ... 2143 Method of determining the feature: E Symbol representing the feature: intron Location of existence: 2156 ... 2666 Method of determining the feature: E AAGCTTGAAC TGGAGCC ATG CCTACCCTCG CGGAGTCATC GGCAAGCCGC ATCATCTTTC 60 TCTCCTTGTC GAGCTCCTCC TACGCAATCT TAGCCTCCCG TATCATCTTC ACCGCCTCGC 120 CACCCCTGCC ATCCATGCCG CCGGTGGCAA CGAGGCCGCT CCTCACAAGC TCGTCGGACA 180 GTTGCTGCGA CCCTACTAGT CCGCCATGAC CGTTGCCCCT CTCCTCGTGG GGTTAGGTCA 240 CTTAGGAGTA TGTCTCTCTC CCTGTCCGGC TTGCAGCATA CACGTATCCG CGCTTTGCTC 300 GCCTGCGTGC ACTCGCCAAG CGATGACTCG CCCTCCCTCG TTGAGGCCAC GGGTGCTAAA 360 GCATATGGTG ACGCCTTCTT CGCACACCTA TGTCACCACC ATTTTCGTCT CCACCTCACC 420 ACTAAGCACA TCCACCTCTG TTGGTTGCAT CGACGTCGCT TCCCTAGCTC CCGTCTCTAG 480 TCCGGATCCT ATTCCTCCTT GGAGACCGAA GCTACCGCAA CCATTGCTCG GTGGTTAGCG 540 AGCGTGGAGC TGTCCTCCCC ACTTTCGCGT CCTCGTTCGC CACCACAGCC ATACTTCGCA 600 TGGTGATGTC TTCTCCTTCA CTCACCGCTA AACTCAGTGC AACCGTTTCT ACCCTAGCCC 660 CGGCCGCCGC TCTCATAGAG GTGAAAGTTC ATTTACATGT AGGTCCCACA TGTTTTATGT 720 TTTTTATTTT TCTTTTACTG ATTAGCATGC CACGTAAATC AAAACAACAA TCCATAGTGT 780 TTTAAGTATT TTTATTTAAT ACGTGAGATG GAGTACAAAA ACGAGAGATG CAAAGTGAAC 840 TTGCTAAAAC ACATTTTCTG GTTGATTACA CTCGCT TGTT GAGCCATTGG ATCGGTCATA 900 GGATTCGTGC TAGCATACTT AATTACGCGT AACTAGTTGT GCTTTATAGG TTACAGGTCG 960 CTAATTAGCG GTCTACTGGA GAACTTTGCT ACTATTTTTT TCTTCACTGC ATGCACTCGA 1020 TCAAGTATGA GTATTTGTAC CGACCAGCGA AACACATATG TAATTAAAGT ATAAATATGT 1080 AATTAGTATA TATTAGTAGT ATATTTAGAC AGTAGTTACA CCCTACATAC ACACCACTTA 1140 CATATATAAT TAGTATGTAA TTTTGTAACT TACATATGTA ATTTTAGTAC TTACATATGT 1200 AATTTTGAGA CTTACATTGT AAATACACTA AAATTACATA TGTAATTTAG TAACCTACAA 1260 TGTAAATACA TGCCGACTAA CTTTTCATGA AAAATATCCC TGTTATAAAT ATAGCTACTC 1320 CCGAACTTTA TTCCTTCTCT GTGAGATATC AGTGGAAACG CTCGGTGGAA TCGGGGGAGT 1380 ATTTGGGAGC ACGCGCCGAC GCGCGCGTCG TGCGTGCCGT CGTCTTTGTC GCGGTGGAGC 1440 GGAGCGCGCC CACTTGCGCG CCTGGGCCGG AGGCGGGCGC GCCGGGGGTT CGGGAATCCC 1500 CTGGAGCCAC ACGTAAAGGC GCGGGCGGGA GGGAGGGAGG GGCCAGCTAG GATAAGGCAC 1560 GCGCGGCCGC TGCGATTGGG GCGCTTGTGA ACACCGGGGC GCCACGTGGA GAGGACGTTA 1620 CACTCCAGCC GCCAAATTTC CACTCCCACA CCCGCGCTCC CCTCCCCTCT CTTTTCCGTG 1680 ATCGCACCTC GCCCACGCGC CCCCCGCCAC ACACAATCTC TGC AGCTCTC CAGCTTCGTT 1740 GGAACTCGCG AATCTCTCTC CGATCCCAGG TAAAGCAGCG AACGACGTCA CGCACGACGC 1800 TGCTCGGTGG ATTTCGTTCC TTGCTGGGGA AAACCATGCA GAGACGAAGG TGAATGATCT 1860 GCTTTTGTGT ACTTGCGTTT ACCAGGTGAA GCGCGACTTG GAGTTGGAGG GGAGATCGAT 1920 CAGGGCCAGG ATG CCG TCG GTT TCG AGG GCC GTG TGC GTG CAG AGG 1966 Met Pro Ser Val Ser Arg Ala Val Cys Val Gln Arg 1 5 10 GCG AGC GGG AAT AAC GGC AGG AGG TGC AGA GAT GGG GCG GCC GCC GCC 2014 Ala Ser Gly Asn Asn Gly Arg Arg Cys Arg Asp Gly Ala Ala Ala Ala 15 20 25 GGC CGC CGA TCC GTC GTG GCG CAG AGA GCG CGG CAC GGC AAG CCG GAG 2062 Gly Arg Arg Ser Val Val Ala Gln Arg Ala Arg His Gly Lys Pro Glu 30 35 40 GTC GCC ATC CGC TCG GGG TCC GGC GGC TCG GCG CGG GGC GGG CAT TGC 2110 Val Ala Ile Arg Ser Gly Ser Gly Gly Ser Ala Arg Gly Gly His Cys 45 50 55 60 TCG CCT CTC AGG GCA GTG GCG GCG CCG ATC CCG ACT ACC AAA AAG 2155 Ser Pro Leu Arg Ala Val Ala Ala Pro Ile Pro Thr Thr Lys Lys 65 70 75 GTAAACCGTG CATGCCGCAG CTCCTCGTTC GTTCGTTGGC TCGCGCGCGC GATTGCTGTG 2215 TTTGACACA C CGTTGACTCT CCTATATTGC AAATGTCCTA ATGTTTTCTT TCAAATACCG 2275 CATTTGTTTT GCAATATTAC TATGGGATGG TTACCTTTTG TTACAGCCAT GCTATTTTGA 2335 TACCATGCTC CTTTGTTAGC TTGGGTTATA ACATGATTAG AGTTTGGACT AGGAATTGCT 2395 TAGCCATGCT TAGTTCAACT AGTTCACAAT GGGGAAAATC ATATTAATGA TTAGACTGTT 2455 GGTTCTAATG GTAATGTTGG ATTAGTACCA CCGCTCTAGT GTTGGTTAAT TAAAATGAAT 2515 CACATGGTGG GCTGTGGGTG CATGGTTTTG CTGGTCGCAC CCATGGCAGT TAAGGACCGG 2575 TTCGCGGGAT GCCCTGGAAG AATTTATCGT ACTTACCACA AGCCAGCGTG GGCAACGGCT 2635 GGGCTTGTAG TGTAACTTTC CTCTAGTCGA C 2666

SEQ ID NO: 28 Sequence length: 8627 Sequence type: Nucleic acid Number of strands: Double strand Topology: Linear Sequence type: Genomic DNA Origin organism name: Oryza Sativa L. Sequence characteristics Symbol: promoter Presence position: 1 ... 760 Method for determining feature: E Feature symbol: CAAT signal Presence location: 639 ... 643 Method for determining feature: E Feature symbol: TATA signal Presence position: 729 ... 734 Feature Method for determining: E Symbol for expressing feature: exon Presence position: 761 ... 915 Method for determining feature: E Feature symbol: intron Presence position: 916 ... 1020 Method for determining feature: E Feature symbol: exon Existence position: 1021 ... 1254 Method of determining feature: E Feature symbol: intron Existence position: 1255 ... 1371 Method of determining feature: E Feature symbol: exon Existence position: 372 ... 1509 Method for determining feature: E Characteristic symbol: intron Presence position: 1510 ... 2967 Method for determining feature: E Characteristic symbol: exon Presence position: 2968 ... 3130 Method for determining feature: E Symbol representing: intron Presence position: 3131 ... 3247 Method for deciding feature: E Feature symbol: exon Presence position: 3248 ... 3483 Method for deciding feature: E Feature symbol: intron Existence position: 3484 ... 3667 Feature Determined method: E Characteristic symbol: exon Presence position: 3668 ... 3796 Characteristic determined method: E Characteristic symbol: intron Present position: 3797 ... 4088 Characteristic determined method: E Characteristic symbol: exon Existence Position: 4089 ... 4268 Method of determining the feature: E Feature symbol: intron Presence position: 4269 ... 4878 Specified method: E Characteristic symbol: exon Existence position: 4879 ... 5016 Characteristic determination method: E Characteristic symbol: intron Existence position: 5017 ... 5204 Characteristic determination method: E Characteristic symbol: exon Existence Position: 5205 ... 5369 Method for determining feature: E Feature symbol: intron Presence position: 5370 ... 5503 Method for determining feature: E Feature symbol: exon Presence position: 5504 ... 5665 Method for determining feature: E Characteristic symbol: intron Presence position: 5666 ... 5804 Characteristic determining method: E Characteristic symbol: exon Present position: 5805 ... 5894 Characteristic determining method: E Characteristic symbol: intron Existing position: 5895 ... 6071 Method of determining the feature: E Symbol representing the feature: exon Location of existence: 6072 ... 6210 Method of determining the feature E Characteristic symbol: intron Presence position: 6211 ... 6576 Characteristic determining method: E Characteristic symbol: exon Existing position: 6577 ... 6754 Characteristic determining method: E Characteristic symbol: intron Existing position: 6755 ... 6906 Feature determining method: E Feature symbol: exon Location: 6907 ... 7036 Feature determining method: E Feature symbol: intron Location: 7037 ... 7317 Feature determining method: E Feature symbol : Exon Existence position: 7318 ... 7446 Characteristic determining method: E Feature symbol: intron Existence position: 7447 ... 7688 Feature determining method: E Feature symbol: exon Existence position: 7689 ... 7826 Feature determining Method: E Feature symbol: intron Location: 7827 ... 8000 Method of determining feature: E Feature symbol: exon Location: 8001 ... 8078 Method for determining features: E Feature symbol: intron Presence location: 8079 ... 8166 Method for determining features: E Feature symbol: exon Presence location: 8167 ... 8258 Method for determining features: E Characteristic symbol: intron Location: 8259 ... 8339 Method for determining characteristic: E Characteristic symbol: exon Location: 8340 ... 8627 Method for determining feature: E AAAACTTCGT CGCTTCCCCG AGGCAAATCG GCAACATGCC GGTCGCCAGCGGGGGTCCAGCG 60 CACCGGTATG CCCGGCATGG TT CACTTTTTGC AGCGCATCGT 120 TGCTGGACAT ACCCTGAGGT TTATCCAGCA ACAAAACGCC GTTAATGTCG CGACCGCGAC 180 GACGAGGACG ACTCATTAGT CCTCCTTGCT GTCGTCCGGG TTAACACGAC GTTCTTCGTC 240 ATGTTTGACC ACGCTGGTCA CCAGGTTTGA CATGCGCATC CCTTCAACCA GAGAGTTGTC 300 GTAGAAGAAG GTCAGTTCCG GCACGATACG CAGGCGCATC GCTTTCCCCA GCAGGCTGCG 360 GATGAAACCA GAAGCTTGCA TTTTATCTAT AACACGTCTA ATTTCTTGTG GGCACTGCAC 420 ATATTCC CCT GGTTTTGATA CAGCGTATCC AAAATTCACT CACACTTAAA AGCTCAAAAA 480 AGCTCCCATT TTAATCACCA CACGTCTAAC AAATTTCTTG TTCACATCCA CAGAAGAAGC 540 TATCCATGCT GTACTTTACA TTGCAGTATT AGACTTTTTA TACTACTTTT ACATTACATT 600 ATTAGACCTT TTTTTAACAC AAAAATCCAC CTACCCAACC AATTTTTTGC CGGGCTGGTC 660 CTCCTCCCCC CGCATGAGCC GCCCGTGCGA TGACGTCTCC CGGTGGGTCA CACCGTCACA 720 CACCGTGCTA TAAATAGGGG GGCTTGGCCT CTCCGCCATG AGCACCACAC TTCACCACTT 780 CGCTTTGCAC AAAGCCTCAG TGCCTCACTA CACTTGCACC GATCACTA ATG GCT CCG 837 Met Ala Pro 1 GCT CAA TGT GCT CGT GTG CAG AGG GTG TTC CAC TTC GGC AAG GGC AAG 885 Ala Gln Cys Ala Arg Val Gln Arg Val Phe His Phe Gly Lys Gly Lys 5 10 76 80 AGC GAG GGC AAC AAG GCC ATG AAG GAC CTG GTAAAAATAA TCGCTGTTGA 935 Ser Glu Gly Asn Lys Ala Met Lys Asp Leu 85 90 94 TACGAGATGA TGATGTTATG AACTTGGAAT TTTGATTGTT TTTTACACTG GTTTTGGTTG 995 GTTGGGGGGG GGla Au Glu GAG AAGu ATG GCG AGC ATC GGG CTG TCG GTG CCG CCG GGG TTC ACG GTG TCG 1095 Glu Met Ala Ser Ile Gly Leu Ser Val Pro Pro Gly Phe Thr Val Ser 105 110 115 ACG GAG GCG TGC CAG CAG TAC CAG GCG GCG GGG AAG ACG CTG CCG GCG 1143 Thr Glu Ala Cys Gln Gln Tyr Gln Ala Ala Gly Lys Thr Leu Pro Ala 120 125 130 135 GGG CTG TGG GAG GAG ATC GTC GAG GGG CTC CAG TGG GTG GAG GAG TAC 1191 Gly Leu Trp Glu Glu Ile Val Glu Gly Leu Gln Trp Val Glu Glu Tyr 140 145 150 ATG GCG GAC CGC CTC GGC GAC CCC GCC CGC CCT CTC CTC CTC TCC GTC 1239 Met Ala Ala Arg Leu Gly Asp Pro Ala Arg Pro Leu Leu Leu Ser Val 155 160 165 CGC TCC GGC GCC GCC GTGCGTATCA CTTACTTATA TTTACTCTC TCTCTCTCTCTT 1294 Arg Ser Gly AlaTCLACCCCTCTCTCTCTCTC CCGTTGCTGA TCGATCCGTG 1354 CGTGCGTGTG TGTGCAG GTG TCG ATG CCG GGG ATG ATG GAC ACG GTG CTG 1404 Val Ser Met Pro Gly Met Met Asp Thr Val Leu 173 175 180 AAC CTG GGG CTG AAC GAC GAG GTG GCG GCG GGA CTG GGC GCG ACC Gly Leu Asn Asp Glu Val Ala Ala Gly Leu Ala Ala Lys Ser 185 190 195 GGC GAC CGC TTC GCG TAC GAC TCC TAC CGC CGT TTC CTC GAC ATG TTC 1500 Gly Asp Arg Phe Ala Tyr Asp Ser Tyr Arg Arg Phe Leu Asp Met Phe 200 205 210 215 GGC AAC GTG GTACGTACTC CTCATCAGAT CACACTCGCC CGGCTCTTCTCT4949 Gly Asn Val 218 ACTCGGCAGCTAGCTCTTCCTGCTCTCTCT AAGTTATTTA 1669 TGCTGCGTCA GGACGGACGG TGGTGGCTGC GGATGCGCAT ACTTTTGTGC GTTGGGTGGG 1729 GCCCGGTTCT TCCGTCTCAT CCTATGTGGG GGGGAATTTG TCCGCACTAG AGTCACTCCA 1789 ACTTCCCAAA GCTTATTGTT CTTTTGTGGC TCTATTTTAC TATTAGAAAA TAGGGAATAA 1849 TTTTAGTAGT ATTTGACTTT AGAAAAGTTA CAAGGAGAAT ATTTTAGTAT TGTGTCAAGA 1909 AAAAAAAGGA AAAAAAAACA AGAGATAGTA TGTCCCTCTC GATCGTATAT GCGATTATGT 1969 GCTAGCAAGG GATTAACATG GAACTTGCTG TCAATATTTG ATCTCCTCGT ACAAAGAATA 2029 CAAGATGTTT TCTTAGGAGA CCTGTACGGC ACTATTGCCG TATTTTGACC ATAGTTCTTT 2089 AATTTTATTG GAAAGGATGA CTGGTGAAGG CGATGGGTGG TTAGCAGTAC AATTTGACAC 2149 TCCTTACTAG CTCAGCTCAC ATATACTCCT ATTTCATGTA ATAAATTAAT TAATCAGTAC 2209 AGGGTCCTCC AACATAGTAC CTGATAACAT CTGC AATATG GAAATATCTG TCTTCCTAGA 2269 AGTTCAAAGA CAAGTTGGTT GCTACTCCAG TCATACAACC AATTACTTTT TTTTCACCTA 2329 TATTCATCAA AGCTTATTTT TTTTGGAAAA AATGTTCTTA ATTTTATTCT TTTTTAGCAA 2389 ATTTAATTTA GAGTAAAAAA CAGTAAGTTT TATTATATTT TTCAAGCGCA CCGTTCACCA 2449 CACCGGTCTC GGCGGTCTGC ACAACTTTTG CCCTTTGCTT GGGGTGCGCA ACCGGTCGGT 2509 CGGTCACCTT GCACCGGACC GGACCGGTCC GGTCGCTGGA GTCGAAGTGG TTCGAACTCC 2569 ACTGCGTCCC GAGCCCTCGA CCACCTCCCT CCTCCTACCA ATCCCAGCCA TGGTTCGACT 2629 CATCACAAGC GCCGCCGCGG CGGCGGCGGC GGCGGCGACG GCGGCGCGGA GGAGAGCGCC 2689 CTCTTACCGC GCCTGAGCCC ATCATGGATG CTAGACCTAT ACAGCTTCGA CTTGCCTTGG 2749 TTGTGATTCG CTGGTAAGAT AAGGCCAGAG GGTTTCGGTA GGATGTCGCA AGGATGAGCC 2809 GCAGAATCGT GAATTTGTGA TGGGTGTTAG TTGTTACTCA ATAGAGGGGA ATTTTTCCTT 2869 TTATTCAGCC CAAAAATTGG TTACAGGTCA TGAATGCTTC CTGGTTCTTG AATGCTACAG 2929 AGATCAGTTC TTAGAATTTT TTTCTCTCTC TTTTCCAG GTC ATG GAC ATT CCT CAT 2985 Val Met Asp Ile Pro His 219 220 GCA CTG TTC GAG GAG AAG CTC GAA GCC ATG AAA GCA GTC AAG GGG CTG 3033 Ala Leu Phe G lu Glu Lys Leu Glu Ala Met Lys Ala Val Lys Gly Leu 225 230 235 240 CAC AAC GAC ACT GAC CTG ACT GCC ACT GAC CTC AAA GAA CTA GTG GCA 3081 His Asn Asp Thr Asp Leu Thr Ala Thr Asp Leu Lys Glu Leu Val Ala 245 250 255 CAG TAC AAG GAT GTC TAT GTT GAA GCT AAG GGA GAG CCA TTC CCC TCT 3129 Gln Tyr Lys Asp Val Tyr Val Glu Ala Lys Gly Glu Pro Phe Pro Ser 260 265 270 272 G GTATGCCCAT AAAGTTTAGGA CTGAATGCTA ATGTACTGTG TTGTTTGCTT 3240 GCCATAG AT CCC AAG AAG CAA CTG CAG CTG GCC GTG TTG GCC GTG TTC AAC 3291 Asp Pro Lys Lys Gln Leu Gln Leu Ala Val Leu Ala Val Phe Asn 273 275 280 285 TCA TGG GATCA ACA CCA AGA AGC ATA AAC AAG ATC 3339 Ser Trp Asp Ser Pro Arg Ala Ile Lys Tyr Arg Ser Ile Asn Lys Ile 290 295 300 ACT GGG CTG AAG GGC ACT GCT GTA AAC GTG CAG ACC ATG GTG TTT GGA 3387 Thr Gly Leu Lys Gly Thr Ala Val Asn Val Gln Thr Met Val Phe Gly 305 310 315 AAC ATG GGG AAC ACC TCT GGC ACG GGT GTG CTC TTC ACT AGG AAC CCA 3435 Asn Met Gly Asn Thr Ser Gly Thr Gly Val Leu Phe Thr Arg Asn Pro 320 325 330 335 AGC ACT GGA GAG AAG AAG CTT TAC GGC GAA TTC CTT GTG AAT GCT CAG 3483 Ser Thr Gly Glu Lys Lys Leu Tyr Gly Glu Phe Leu Val Asn Ala Gln 340 345 350 351 GTATGGCTCA CACCCTGTTG CTCCTATTTG CATCTAGCTG CTAACTGTTG CACACCTGTG 3543 GTTTGTGTGT ATGAATCAAT GTATGAGCAA TATGTTTCCT GCTGATCGTA TTGGGTTATG 3603 CAAGCGGCGA TAATTCATAT ATACCCGAAC GATTTCTTAT AATGCAATAT TATTTCTTCT 3663 GCAG GGT GAA GAT GTG GTT GCT GGA ATC AGA ACT CCA GAG GAT CTT GAT 3712 Gly Glu Asp Val Val Ala Gly Ile Arg Thr Pro Glu Asp Leu Asp 352 355 360 365 GCT ATG AGG GAC CAC ATG CCG GAG CCT TAT GAA GAG CTC GTT GAG AAC 3760 Ala Met Arg Asp His Met Pro Glu Pro Tyr Glu Glu Leu Val Glu Asn 370 375 380 TGC AAA ATT TTG GAG AGT CAC TAT AAA GAA ATG ATG GTACAATATT 3806 Cys Lys Ile Leu Glu Ser His Tyr Lys Glu Met Met 385 390 394 CTAATTTGCT TTATGCATCA ATACCTTATT AGATAATGGG ATTAAGGCAG ATTAAGGCTCA TACT ATACTCGAGC ATTGTCTTGG TGATATCTCA TTACCTCCAA TAGCTTGCAC ACAGAAATAA 3986 GTGATTTGAA ACGTCTTGAT CATGCTCTTG GTTTAGCAAC TAGGTAATGT GCCTCCATTA 4046 TTTTTTTTTG GGAGACTAAC TTAATTTTCA TGTATAATGC AG GAT ATT GAA TTT 4100 Asp Ile Glu Phe 395 ACT GTT CAG GAA AAT AGG CTT TGG ATG CTT CAG TGC AGA ACA GGA AAG 4148 Thr Val Gln Glu Asn Arg Leu Trp Met Leu Gln Cys Arg Thr Gly Lys 400 405 410 CGC ACA GGA AAA GGT GCT GTA AAG ATT GCT CTA GAC ATG GTT AAC GAG 4196 Arg Thr Gly Lys Gly Ala Val Lys Ile Ala Leu Asp Met Val Asn Glu 415 420 425 430 GGC CTT GTT GAG CGC CGC ACA GCA CTT AAG ATG GTA GAA CCA GGT CAC 4244 Gly Leu Val Glu Arg Arg Thr Ala Leu Lys Met Val Glu Pro Gly His 435 440 445 CTG GAT CAG CTT CTT CAT CCT CAG GTATTGTATA ACTAAGAGGC298 Leu Asp Gln Leu Leu His Pro Gln 450 454 TATAAACTCA TGCACCACAT AAATGGACAC GTACAATTTC ATAATACCTC TGTTATCCCA 4358 AAATGCCGTG TTGCTCTTTT AGAACAATTT TGTTGGGTTGGTGTATTGAT GTCATAGATA 4AG T418CA TT AAATGTGTGT TTGCGATCTA TTAGTGAAAT TTGATACGCA TGTCTGCTTT 4538 AATTCTGAAC ATCAAAATCT TTATGTTATA TTTTTCCACA AAATAGGTAG AGTGTTTGTT 4598 ACTTGAACTC TGAGCATTTT GAATTATTTG GAATGAGTTT TAGCAAACAA CAGAGATTAT 4658 CTCATTACGT CTTGTGTAGT AAAGTATAAT ACTATTATCA TAACTAACAA ATATCAAACA 4718 TAATCACACA AGTATTGATG TTGGTAACTG TTGTTTTAAA ATTTTGTTGC CTGCTAGTTT 4778 CTGCACAGGT CTTGGACATT GCGTACTTGA GTTTCAGTTC ACAACTAAAT TAATGGCAGT 4838 TATTGATATC TCACTTTTAA TCTTTTGTTA TGAAAACCAG TTT GAG AAC CCA TCA 4893 Phe Glu Asn Pro Ser 455 GGG TAT AAA GAC AAA GTT ATT GCC ACG GGC TTA CCT GCA TCA CCT GGG 4941 Gly Tyr Lys Asp Lys Val Ile Ala Thr Gly Leu Pro Ala Ser Pro Gly 460 465 475 475 GCT GCT GTG GGC CAA ATT GTA TTC ACT GCC GAG GAT GCT GAA GCA TGG 4989 Ala Ala Val Gly Gln Ile Val Phe Thr Ala Glu Asp Ala Glu Ala Trp 480 485 490 CAT GCC CAA GGG AAA GAT GTT ATT CTG GTACGTCCTG ATCATCTGTC 5036 His Ala Gln Gly Lys Ile Leu 495 500 TTTCTAGGAC AAATTTGTTT GTTTCGTCCA TGTTAGAATA TTAAACAGCT GCTTATAGGA 5096 AAAAAAATAG ATTTA TCACA GCTTTATTCA AGCTGCACTT GAAAACTCCA CATTGCATCG 5156 TTGCATTTGG TGTATCTTGA CATTTTCCTG TCCATTATGA ATGTAAAG GTG AGG ACA 5213 Val Arg Thr 501 GAG ACC AGC CCA GAG GAT GTT GGT GLC GTG Glu Gat GLA GATT GGA Gru Ser Gly Ile 505 510 515 CTT ACA GCA AGA GGT GGT ATG ACC TCT CAC GCT GCT GTT GTT GCG CGC 5309 Leu Thr Ala Arg Gly Gly Met Thr Ser His Ala Ala Val Val Ala Arg 520 525 530 535 GGA TGG GGC AAA TGT TGT GTG TCA GGA TGC TCA AGC GTC CGT GTA AAT 5357 Gly Trp Gly Lys Cys Cys Val Ser Gly Cys Ser Ser Val Arg Val Asn 540 545 550 GAT GCG TCC AAG GTGATTATAC TAGATTCTTG CACCACACAA ACATTTAAGA 5409 Asp Ala Ser Lys 555 GTTCACTTCATCCTT GCTTAATTTG CTGTGTCTCT ACAG ATT GTA GTG ATT GAA GAC 5521 Ile Val Val Ile Glu Asp 556 560 AAG GCG CTG CAT GAA GGT GAG TGG CTA TCG TTG AAT GGA TCA ACT GGT 5569 Lys Ala Leu His Glu Gly Glu Trp Leu Ser Leu Asn Gly 565 570 575 GAA GTG ATC ATT GGC AAG CAG CCA CTC TGC CCA CCA GCC CTT AGT GGT 5617 Glu Val Ile Ile Gly Lys Gln Pro Leu Cys Pro Pro Ala Leu Ser Gly 580 585 590 GAT TTG GAA ACT TTC ATG TCT TGG GTG GAT GAA GTT AGG AAA CTC AAG 5665 Asp Leu Glu Thr Phe Met Ser Trp Val Asp Glu Val Arg Lys Leu Lys 595 600 605 609 GTAAGCAAAA TATGTTTCAA GTTGAAGTCA GCTGAAAAAG GGACATTAGT TTTATTTACT 5725 CACTAAGGTC AAGGCTACCT AGTT GAT GCT AGT GCT AGT GTC AGT GTC AGT GTCCATG ATC Asp Thr Pro Glu Asp 610 615 GCA ACA ACA GCC AGA CAG AAT GGG GCA GAA GGA ATT GGG CTA TGC CGG 5882 Ala Thr Thr Ala Arg Gln Asn Gly Ala Glu Gly Ile Gly Leu Cys Arg 620 625 630 635 ACT GAA CAT ATG GTTTGTATTA TTAATGCTCT TTTTTTTTCT 5934 Thr Glu His Met 639 CTTTTTTGCT AGGATGATAT GTGCCTGAAC TTTTTAGTTG AGTTTAACAT TTAGAAGCTA 5994 GATTCATGGA TGCAACTCTT CTCTTCTAGT CAATGCAG GAT GACATAT GTCATGT ATCTTCCA GTCAT GTCATTCA GACTCAT GTCATTCA GTCAT TCA TCTTChe g Ile Lys Ala Val 640 645 650 AGG CAG ATG ATT ATG GCT TCA AGT CTT GAA CTG AGG CAG AAA GCA CTA 6152 Arg Gln Met Ile Met Ala Ser Ser Leu Glu Leu Arg Gln Lys Ala Leu 655 660 665 GAT CGC CTT TTG CCT TAT CAG AGG TCT GAC TTT GAA GGC ATC TTC CGT 6200 Asp Arg Leu Leu Pro Tyr Gln Arg Ser Asp Phe Glu Gly Ile Phe Arg 670 675 680 GCA ATG GAT G GTATGCGAAA TTTTTACCAT AATAATATAT AGT ATCATTATCAGA TATGAA TATGAA TATGAA TATGAA TATGAA TATGAAGAT TAAGTTAATT GATTGTTCTA GTGTAGATGC AACGTTATAA ATTTGCAAAA GGGATGCAAT 6370 ATACTTTTAG AAGATTGCCT ATATTATCTT AACATATTAT GCATCATGTA AGTTAATGGG 6430 CACGATTCTG CTATGATTCT TCTTTTATGG AAAGGGGTAC ATTGATGGAT GTATGATTCA 6490 TTCAACATAG TCTTAATTCA TTGCATCTTG CACACAACAT GCTTGGTCAT ACAGGCGTTT 6550 AAAAATGGTT TTTTGTTGCA TTCCAG GG CTT CCG GTA ACT ATT AGA CTC TTG 6602 Gly Leu Pro Val Thr Ile Arg Leu Leu 686 690 GAT CCT CCA CTT CAT GAG TTC CTT CCA GAA GGC CAT GTT GAG GAT ATG 6650 Asp Pro Pro Leu His Glu Phe Leu Pro Glu Gly His Val Glu Asp Met 695 700 705 710 GTG CGT GAG CTA TGC TCT GAA ACT GGA GCA GCT CAG GAT GAT GTC CTT 6698 Val Arg Glu Leu Cys Ser Glu Thr Gly Ala Ala Gln Asp Asp Val Leu 715 720 725 GCA AGA GTA GAA AAA CTT TCC GAA GTA AAT CCA ATG CTT GGT TTC CGT 6746 Ala Arg Val Glu Lys Leu Ser Glu Val Asn Pro Met Leu Gly Phe Arg 730 735 740 GGG TGC AG GTTGGTTTTT GCACTTTGCT ATTCAGTGTT GAATTAGGTTCTC GTT Cycat Arg 745 TTTCCTTTTCCATT AG 6906 G CTT GGT ATA TCA TAC CCT GAA TTG ACT GAA ATG CAA GCC CGT GCC ATC 6955 Leu Gly Ile Ser Tyr Pro Glu Leu Thr Glu Met Gln Ala Arg Ala Ile 746 750 755 760 TTT GAA GCT GCT ATA ACC ATG ACC AAC CAG GGT ATT CAA GTC TTT CCG 7003 Phe Glu Ala Ala Ile Thr Met Thr Asn Gln Gly Ile Gln Val Phe Pro 765 770 775 GAG ATA ATG GTT CCC CTT GTT GGA ACT CCT CAG GTATGTGTTT 7046 Glu Ile Met Val Pro Leu Val Gly Thr Pro Gln 780 785 788 GCTTTCTTCT TTTCATTCAT AGTACCTGAC TTGTGTAATT GACTGTCTGT GAAGGTTCTG 7106 C AATTTAGAT GATAACTTAT ATTTACATGA ATTCCAATTT CTAGAATCAT GCAAGTATTA 7166 AATCACGTGT GAATGGAACA TTGACTTCAT CCGGGAAATG GTTCATTCCA TTTCTTCATA 7226 TTTACCTTCT TTGATGTTCT AATATATTTG ATTACATTGT TCTTTTCGGT TGGTTTTCAA 7286 ACATTGACAT TTGAGTATTT TGCTGTGACA G GAA TTG GGG CAT CAA GTG GAT 7338 Glu Leu Gly His Gln Val Asp 789 790 795 GTT ATC CGT CAA ATT GCC AAC AAG GTG TTC ACA GAC ATG GGA AAA ACT 7386 Val Ile Arg Gln Ile Ala Asn Lys Val Phe Thr Asp Met Gly Lys Thr 800 805 810 ATT GGC TAC AAA GTT GGA ACT ATG ATT GAA ATT CCC AGG GCA GCT TTA 7434 Ile Gly Tyr Lys Val Gly Thr Met Ile Glu Ile Pro Arg Ala Ala Leu 815 820 825 GTG GCT GAT GAG GTAAGAAACA CTGATAGCTC CCTTCTTTTT TGAAAATCTT 7486 Val Ala Asp Glu 830 831 CAGTATGGTT ATTGTCATTC ACATGTAGAC AGAACTGTAC ATGCAACATG GCTAGTAATA 7546 GAATCTTGGA TATTACCCTA AAGGATATTT TGACTTGAAA TAGTGAATGA TTATAAAAAT 7606 ATTCAAAGTT CAAATGATGA GTTTATGTTT TCTTTAAACC TATAAGCTGA CAGTGCTGTG 7666 TTGGCTGCAC ATTTATTGAC AG ATA GCA GAG CAG GCT GAG TTC TTC TCC TTT 7718 Ile Ala Glu Gln Al a Glu Phe Phe Ser Phe 832 835 840 GGA ACA AAC GAC CTA ACA CAG ATG ACA TTT GGT TAC AGT AGG GAT GAT 7766 Gly Thr Asn Asp Leu Thr Gln Met Thr Phe Gly Tyr Ser Arg Asp Asp 845 850 855 GTG GGG AAG TTC CTT CCC ATC TAT CTG TCT CAG GGT ATC CTC CAG CAT 7814 Val Gly Lys Phe Leu Pro Ile Tyr Leu Ser Gln Gly Ile Leu Gln His 860 865 870 GAC CCC TTT GAG GTAAATTTGT ACAGCCAAAA TGGCCTCCCT TGATAAACAC 7866 Asp Pro CAheClu CATATTCTTT TCCCAACTCT 7926 CGAGTCCTGA CTTCTGATTT GGTGCAATCT GACCAATTTC TATCATGTAA CATACCTGTT 7986 GATGAATATA TTAG GTG CTT GAT CAG AGG GGA GTT GGG GAG CTG GTT AAG 8036 Val Leu Asp Gln Arg Gly Val 8G ACA GCA Val GSA CGA GCT CCT AAC TTG AAG 8078 Leu Ala Thr Glu Arg Gly Arg Lys Ala Arg Pro Asn Leu Lys 890 895 900 903 GTTAGCCTTT CACTGGAGCT GAGGACATCA CCTTGTATAG AATCCATGGG TTTCTTATAT 8138 TGAA Gly GTG GTC 904 905 910 GGA GAG CCT CTG TCA GTC GCT TTC TTT GCG AAG GCT GGG CTG GAC TAT 8238 Gly Glu Pro Leu Ser Val Ala Phe Phe Ala Lys Ala Gly Leu Asp Tyr 915 920 925 GTT TCT TGT TCG CCT TTC AG GTCGGTTGAG TGATGAAAAT ValGAGAAT ATGAGAAT CGA TGA Ser Pro Phe Arg 930 934 ACTCATAAAT TCACAGAACA ATCACTAACT GTTATTAACT CTCTTTGGCA G 8339 G GTC CCA ATT GCT AGG CTA GCT GCA GCT CAG GTG CTC CTC TGATGATGAC 8389 Val Pro Ile AlaTGLACT CGCTCGTCGATCTGCTGACCTGCTAGCCTGCTAGCT GCACCTGGTG AAGAATAATA 8449 AATACATAGC CATGGAATGG TGGAAACTCT GCTGCCTGTC AAAGGCGCCG TTGTATGTAG 8509 TATGCATGTT CGCATGTAAG CCTATGTGGT GGTAGTTTTC TGAAAATTTC GCTTGAACTG 8569 CCACGGTAAT GTACCAAACA GCGATGACGA AGAAAGA CGAAAGA

[Brief description of drawings]

FIG. 1 is a schematic diagram of a rice PPDK gene having two promoters.

FIG. 2 is a diagram of Northern hybridization showing the expression level of PPDK mRNA in each organ of rice.

FIG. 3 PPDKmRN expressed in spikelets and green leaves of rice
FIG. 5 is a diagram of Northern hybridization showing a comparison of the sizes of A.

Claims (7)

[Claims] 1. A cDNA of a rice pyruvate orthophosphate dikinase gene having a nucleotide sequence encoding the amino acid sequence represented by SEQ ID NO: 25 in the sequence listing. 2. SEQ ID NOS: 2, 3 and 6 to 24 in the sequence listing
The cDNA according to claim 1, which has a base sequence represented by: 3. A cDNA of a rice pyruvate orthophosphate dikinase gene having a nucleotide sequence encoding the amino acid sequence represented by SEQ ID NO: 26 in the Sequence Listing. 4. The cDNA according to claim 3, which has the nucleotide sequences represented by SEQ ID NOs: 5 to 24 in the sequence listing. 5. A promoter 1 having a nucleotide sequence represented by SEQ ID NO: 1 in the sequence listing and expressed in the leaf blade of rice. 6. A promoter 2 having a nucleotide sequence represented by SEQ ID NO: 4 in the sequence listing, which is expressed in spikelets of rice. 7. A genomic DN comprising a pyruvate orthophosphate dikinase gene of rice having the nucleotide sequences represented by SEQ ID NOs: 27 and 28 in the sequence listing and a promoter thereof.
A fragment.