JPH07140143A - Method and apparatus for inspecting stool - Google Patents

Abstract

PURPOSE:To realize the positive detection of human occult blood by a method wherein human hemoglobin is captured by an antihuman hemoglobin antibody and attracted magnetically and then excessive components are discharged before luminescent reaction takes place. CONSTITUTION:A liquid dispersed with dejection is taken into a sample introduction chamber 33. A diagnostic reagent is then fed into the sample introduction chamber 33. Antihuman hemoglobin antibodies in the reagent capture human hemoglobin in the sample. Magnetic particles in the antihuman hemoglobin antibody are attracted to an electromagnet 40. A cleaning liquid is then fed into the sample introduction chamber 33 and a luminescent reaction reagent is added inside sample introduction chamber 33 through a reagent supply path 37. The quantity of light emitted through the luminescent reaction is detected by an optical detector 34.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a toilet bowl and device for health judgment. More specifically, the present invention relates to a toilet bowl and device for determining a health condition by utilizing an occult blood reaction in feces, for example.

[0002]

2. Description of the Related Art In diagnosing and treating diseases such as ulcer, carcinoma, tuberculosis, dysentery, and typhoid, occult blood reaction of feces may be examined. In particular, 50-77% for gastrointestinal ulcers,
87% of cancer types show positive occult blood test, which is considered to be a powerful medical guideline.

As a method for examining the occult blood reaction of feces, there are a spectroscopic method, a catalytic method, a hemin crystal test method and the like. In the spectroscopic method, the occult blood reaction can be accurately examined by observing the unique absorption line, but in practice, the catalytic method is mainly used.

The principle of the catalytic method adopted to observe the occult blood reaction in feces will be described. When occult blood is present in the feces, when acetic acid is added to hemoglobin in the erythrocytes of this occult blood, the hemoglobin is converted to hematin by acetic acid, and when hydrogen peroxide acts on this hematin, oxygen is generated. Then, in this state, when an organic drug (eg, orthotrizine, guaiac) is added,
This oxygen oxidizes the organic drug and changes its color to blue-green.

Therefore, the presence of occult blood in the feces can be visually confirmed by visually observing the blue-green color.

[0006]

Since the color reaction by the above-mentioned catalytic method is induced not only by human hemoglobin but also by hemoglobin of meat and fish, and peroxidase in plants such as horseradish and radish, false positives are likely to occur. Therefore, in order to make an accurate diagnosis, it was necessary to carry out a troublesome diet restriction from 2 to 3 days before the stool collection.

[0007]

A fecal test method according to claim 1, wherein a diagnostic substance obtained by binding an anti-human hemoglobin antibody to a magnetic substance is contacted with a sample, and human hemoglobin in the sample is treated with the diagnostic substance. A step of capturing the anti-human hemoglobin antibody, a step of holding the anti-human hemoglobin antibody on the surface of the magnetic flux generator by a magnetic flux, and a step of supplying a washing solution while holding the anti-human hemoglobin antibody on the surface of the magnetic flux generator. , Supplying a peroxide and a luminescent reagent to the surface of the magnetic flux generator holding the anti-human hemoglobin antibody, causing the hemoglobin captured by the anti-human hemoglobin antibody to cause a luminescence reaction, and measuring the amount of luminescence with a light amount detector And a step of detecting the amount of human hemoglobin based on the output signal of the light amount detector.

The magnetic particles have a particle diameter of 0.1 to 0.1.
A metal powder or a ceramic powder of about 10 μm is suitable, but a magnetic polymer material may be used.

According to a second aspect of the present invention, in the fecal inspection method according to the first aspect, after the amount of light emission is detected, the generation of magnetic flux from the magnetic flux generator is stopped and a cleaning liquid is supplied to the surface of the magnetic flux generator. To do.

According to a third aspect of the feces inspection device, a cylinder having an open tip, a plunger inserted in the cylinder,
A sample introducing chamber that is provided at the tip of the plunger and communicates with the side surface of the plunger, a reagent supply passage and a washing liquid supply passage that communicate with the sample introducing chamber, and faces the sample introducing chamber. The magnetic flux generator and the photodetector means provided as described above are provided.

According to a fourth aspect of the feces inspection apparatus, a cylinder having an open tip, a plunger inserted in the cylinder,
A shutter that is provided at the tip of the cylinder and defines a sample introduction chamber at the tip of the cylinder when closed, and a reagent supply passage and a cleaning liquid supply passage that communicate with the sample introduction chamber. A magnetic flux generating means and a light detecting means provided so as to face the sample introducing chamber,
It is equipped with.

[0012]

In the method of the present invention, when a sample (feces or a liquid containing it) is contacted with a diagnostic substance, only human hemoglobin in the sample binds to the anti-human hemoglobin antibody of the diagnostic substance.

When a magnetic flux is generated from the magnetic flux generator, the diagnostic substance has a magnetic substance, so that the diagnostic substance is magnetically adsorbed and held on the surface of the magnetic flux generator.

Therefore, a cleaning liquid is caused to flow on the surface of the magnetic flux generator to remove excess substances, and then a peroxide and a luminescent reagent are supplied. As a result, the luminescent reaction is carried out using the human hemoglobin retained by the anti-human hemoglobin antibody as a catalyst. By detecting this luminescence amount, human hemoglobin in the sample can be detected.

Thus, the state of occult blood in the feces can be easily known, and the measurement of this occult blood can be used as a guideline for determining gastric cancer, colon cancer and the like.

In the method of the second aspect, after the measurement, the magnetic flux is extinguished and a cleaning liquid is caused to flow, so that the diagnostic substance is flowed off from the surface of the magnetic flux generator.

In the stool inspection apparatus according to the third and fourth aspects, the sample (feces or a liquid containing the same) is taken into the sample introduction chamber, the diagnostic substance-containing liquid is supplied, and then a magnetic flux is generated from the magnetic flux generator. As a result, the anti-human hemoglobin antibody is magnetically adsorbed and held on the surface of the magnetic flux generator. Next, after supplying a cleaning liquid to remove excess substances, for example, a peroxide and a luminescent reagent are added. Then, the light emission amount is detected by the photodetector.

After the measurement is completed, the washing liquid is supplied to the sample introducing chamber to wash it.

According to the third aspect, the sample introducing chamber at the tip of the plunger becomes a dark chamber by pulling the plunger into the cylinder. According to the fourth aspect, the sample introducing chamber is brought into the dark chamber state by pulling the plunger into the cylinder and closing the shutter.

[0020]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will be described below with reference to the drawings. FIG. 1 is a cross-sectional view of a toilet bowl for health determination equipped with an apparatus according to an embodiment of the present invention.

The Western-style toilet 1 is provided with a toilet bowl 2, a water seal portion 3, a drain port 4, a rim water passage 5, and the like. A tank cover 6 is installed on the rear upper surface of the western-style toilet 1, and a low tank (not shown) is installed in the tank cover 6. A stool inspection device 7 is installed in the tank cover 6. The stool inspection device 7 includes a cylinder 7a, a rod 7b, a sensing unit 7c provided at the tip of the rod 7b and an ultrasonic transducer 7d, and a pinion 7e and a motor 7f for moving the rod 7b forward and backward. . The rod 7b is provided with a rack meshed with this pinion 7e,
The rod 7b is advanced and retracted by the rotation of the motor 7f. In addition, 8 is a toilet seat and 9 is a toilet lid.

In this toilet, after the feces have been put into the water-sealed portion 3, the rod 7b is moved forward and the ultrasonic transducer 7d is operated to break up the feces. And the sensing unit 7c
Inspect the stool by.

FIG. 2 shows a Western-style toilet equipped with another embodiment of the apparatus. A toilet seat box 11 is installed on the upper rear surface of the Western-style toilet 10, and a toilet seat 12 and a toilet lid 13 are pivotally mounted on the toilet seat box 11. It is supported. A stool inspection device 14 is installed in the toilet seat box 11. This stool inspection device 14
Is a multi-stage cylinder type that is extended by water pressure. When the valve 15 is opened, the stool inspection device 14 is extended as shown by the water supply pressure from the water supply pipe 16, and the tip of the stool inspection device 14 is immersed in the water seal portion 17. The feces inspection device 14 is provided with an ultrasonic transducer and a sensing unit (both not shown) at its tip.

FIG. 3 shows a Western-style toilet 20 equipped with devices of different embodiments.
Is attached. Most of the stool inspection device 22 is disposed in the toilet bowl internal space 23 behind the toilet bowl, and only the tip thereof projects into the water seal portion 21.

The water seal portion 21 is provided with a propeller 24 for disintegrating feces. Reference numeral 25 is a motor for rotating the propeller 24.

The shaft of the propeller 24 and the wall surface portion of the water seal portion through which the feces inspection device 22 penetrates are sufficiently sealed (not shown).

Referring to FIGS. 4 to 8, the stool inspection devices 7, 1
Specific examples of Nos. 4 and 22 will be described.

As shown in FIGS. 4 and 7, the stool inspection device 30 is provided with a plunger 32 in a cylindrical cylinder 31 so that the plunger 32 can move forward and backward, and a sample introducing chamber 33 is formed so that the tip of the plunger 32 is recessed from the side surface. Is provided.

In the plunger 32, a cleaning liquid supply passage 36 for supplying a cleaning liquid to the sample introduction chamber 33, reagent supply passages 37, 38 for supplying a reagent, and a drainage passage 4
2 and are provided. The plunger 32 is provided with an optical fiber 39. The tip of the optical fiber 39 faces the sample introducing chamber 33, and the photodetector (photodiode or photomultiplier) 34 is connected to the rear end. An electromagnet 40 is provided on the plunger 32 so as to face the sample introduction chamber 33. Reference numeral 41 indicates a lead wire for energizing the electromagnet 40.

When inspecting a stool, this stool inspection device 3
With the tip of 0 immersed in the water seal,
As shown in FIG. 5, the dispersed water of feces is taken into the sample introducing chamber 33. Next, the plunger 32 is retracted into the cylinder 31 as shown in FIG. Therefore, the diagnostic reagent is supplied from the reagent supply passage 38 into the sample introduction chamber 33.
Then, the anti-human hemoglobin antibody in this diagnostic reagent captures human hemoglobin in the sample. Then
The electromagnet 40 is energized. The anti-human hemoglobin antibody is carried on magnetic particles, and the anti-human hemoglobin antibody is firmly held on the surface of the electromagnet 40 by adsorbing the magnetic particles on the electromagnet 40.

Therefore, the cleaning liquid is supplied from the cleaning liquid supply passage 36 into the sample introducing chamber 33, and the excess substance is discharged into the liquid discharge passage 42.
To be discharged from.

Next, the luminescent reaction reagent is added to the sample introducing chamber 33 from the reagent supply path 37. Then, the amount of light generated by the luminescence reaction is detected by the photodetector 34, and a fecal test is performed. A peroxide and a luminol reagent are used as this reagent, the amount of luminescence is detected by a photomultiplier or a photodiode, and the amount of human hemoglobin present in feces is detected.

Since the chemiluminescence of the luminol reagent is based on excited 3-aminophthalic acid produced by the oxidation of the luminol reagent, the chemiluminescence reaction is carried out using an oxidizing substance such as hydrogen peroxide. Let The luminescence reaction of luminol is shown below.

[0034]

[Chemical 1]

Therefore, when occult blood (human hemoglobin) is present in the feces, chemiluminescence occurs due to the reaction, and the occult blood amount is calculated from the luminescence amount.

After detecting the amount of light emission, the energization of the electromagnet 40 is stopped and the cleaning liquid is supplied from the supply passage 36,
The inside of the sample introduction chamber 33 is thoroughly washed. By demagnetizing the electromagnet 40, the magnetic substance also flows out from the sample introducing chamber 33 together with the cleaning liquid.

Various mechanisms such as a rack and pinion mechanism and a fluid pressure cylinder mechanism can be adopted as the mechanism for moving the plunger 32 forward and backward.

In FIGS. 4 to 8, the optical fiber 39 is provided on the plunger 32, so that the photodetector 34 is not affected by the electromagnet 40. In addition,
If the photodetector 34 is not affected by magnetism, the photodetector 34 may be installed so as to directly face the sample introduction chamber 33.

9 and 10 show another embodiment of the apparatus of the present invention. In this embodiment, a shutter 44 is provided at the tip of the cylinder 31. A motor 46 is connected to the shutter 44 via a screw shaft 45. Screw shaft 4
The nut 47 and the motor 46 meshing with the
It is fixed to.

The motor 46 causes the shutter 44 to perform only a half rotation, and when the motor 46 is rotated in the positive direction from the state shown in the drawing, the shutter 44 covers the tip of the cylinder 31 and is screwed upward in the drawing. As it is advanced, it comes into close contact with the tip surface of the cylinder 31.

In the apparatus of this embodiment, as shown in FIG. 9, the plunger 32 is projected and the diagnostic substance is caused to flow out from the reagent supply passage 38 while being immersed in the sample liquid, and the electromagnet 40 is energized. As a result, the anti-human hemoglobin antibody bound to human hemoglobin is adsorbed on the surface of the electromagnet 40. Therefore, as shown in FIG. 10, the plunger 32 is retracted into the cylinder 31 and the shutter 44 is closed. After flowing the cleaning liquid into the sample introducing chamber 33, the luminescent reagent is supplied into the sample introducing chamber 33 to cause a luminescent reaction.
Human hemoglobin is detected by detecting the amount of emitted light with the photodetector 34. After the measurement is completed, the electromagnet 40 is demagnetized and the cleaning liquid is flown.

In the above embodiment, the electromagnet 40 is provided so as to directly face the sample introducing chamber 33. However, as shown in FIG. 11, the electromagnet is arranged away from the sample introducing chamber 33, and the electromagnet and the sample introducing chamber are separated from each other. A material 45 having a high transmittance may be provided between the two.

In the embodiment shown in FIGS. 4 to 8, an electromagnet or a high-permeability material is provided on the inner surface of the tip portion of the cylinder 31 (the surface facing the sample introduction chamber 33 when the plunger 32 is retracted). May be.

It is obvious that the present invention can also be applied to a case where a sample is collected from the water-sealed portion of the toilet bowl to the separation system path and guided to the measuring device.

In order to disintegrate the feces in the water seal part,
Water or air may be jetted into the water seal portion.

[0046]

According to the stool inspection method and device of the present invention,
A sample is introduced into the sample introduction chamber, human hemoglobin is captured by an anti-human hemoglobin antibody and magnetically adsorbed and held, and then excess components are allowed to flow out, and in this state, a luminescent reagent is supplied to cause a luminescent reaction, The human occult blood can be reliably detected.

[Brief description of drawings]

FIG. 1 is a cross-sectional view of a toilet bowl for health determination including an apparatus according to an embodiment of the present invention.

FIG. 2 is a cross-sectional view of a toilet bowl for health determination including another embodiment of the apparatus.

FIG. 3 is a cross-sectional view of a toilet bowl for health determination including a device according to another embodiment.

FIG. 4 is a side view of the stool inspection apparatus according to the embodiment.

5 is a cross-sectional view taken along the line VV of FIG.

FIG. 6 is a cross-sectional view of the feces inspection device when the plunger is projected.

FIG. 7 is a perspective view of a tip portion of the feces inspection device.

8 is a sectional view taken along the line VIII-VIII in FIG.

FIG. 9 is a cross-sectional view of a stool inspection device according to another embodiment.

10 is a cross-sectional view showing the operation of the apparatus of FIG.

FIG. 11 is a cross-sectional view of a fecal inspection device according to yet another embodiment.

[Explanation of Codes] 1,10,20 Toilet bowl 2 Toilet bowl 3,17,21 Water sealing part 4 Drainage port 7,14,22,30 Stool inspection device 31 Cylinder 32 Plunger 33 Sample introduction chamber 34 Photodetector 36 Supply of cleaning liquid Channel 37, 38 Reagent supply channel 39 Optical fiber 42 Drainage channel 45 High permeability material

Claims (4)

[Claims] 1. A step of contacting a sample with a diagnostic substance obtained by binding an anti-human hemoglobin antibody to a magnetic substance, and capturing human hemoglobin in the sample with the anti-human hemoglobin antibody of the diagnostic substance, The step of holding the hemoglobin antibody on the surface of the magnetic flux generator by the magnetic flux, the step of supplying the cleaning solution with the anti-human hemoglobin antibody held on the surface of the magnetic flux generator, and the step of the magnetic flux generator holding the anti-human hemoglobin antibody A step of supplying a peroxide and a luminescent reagent to the surface, causing a luminescence reaction by the hemoglobin captured by the anti-human hemoglobin antibody, and detecting the luminescence amount with a light quantity detector. A fecal test method comprising detecting human hemoglobin on the basis of an output signal of the container. 2. The stool inspection method according to claim 1, wherein after the amount of light emission is detected, the generation of magnetic flux from the magnetic flux generator is stopped and a cleaning liquid is supplied to the surface of the magnetic flux generator. 3. A cylinder having an open tip, a plunger inserted in the cylinder, a sample introducing chamber provided at the tip of the plunger and communicating with a side surface of the plunger, and the sample. A stool inspection device comprising a reagent supply passage and a washing liquid supply passage communicating with the introduction chamber, and a magnetic flux generation means and a light detection means provided so as to face the sample introduction chamber. 4. A cylinder having an open tip, a plunger inserted into the cylinder, and a tip part of the cylinder, which is provided at the tip part of the cylinder and defines a sample introduction chamber at the tip part of the cylinder by a closing operation. A stool inspection device comprising a shutter to be formed, a reagent supply passage and a washing liquid supply passage communicating with the sample introducing chamber, and a magnetic flux generating means and a light detecting means provided so as to face the sample introducing chamber. .