JPH07133215A - Composition for oral cavity application - Google Patents

Abstract

PURPOSE:To provide a composition for oral cavity application having remarkable effect for suppressing the formation of dental plaque and containing dextranase in high stability. CONSTITUTION:This oral cavity composition is produced by compounding triclosan to an oral cavity composition containing dextranase in combination with alkyloyl sarcosinate.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a composition for the oral cavity, which has improved plaque formation suppressing effect and dextranase stabilizing effect.

[0002]

2. Description of the Related Art Conventionally, the problems to be solved by the invention
Dextranase is known to have a plaque formation-inhibiting effect, and an oral composition containing the same has been proposed (Patent No. 782154). Further, a composition for oral cavity in which dextranase and sodium lauroyl sarcosine are used in combination for stabilizing dextranase has also been proposed (JP-A-56-123910).

However, these oral compositions still have room for improvement in terms of the plaque formation-inhibiting effect and the stability of dextranase, and therefore have high plaque formation-inhibiting ability and sufficient stability of dextranase. It is further desired to develop an oral composition having

[0004]

Means and Actions for Solving the Problems As a result of intensive studies conducted by the present inventor in order to meet the above-mentioned demands, as a result, an additional oral composition containing dextranase and alkyloyl sarcosinate was further mixed with triclosan. As a result, they have found that the plaque formation-inhibiting effect and the dextranase-stabilizing effect of the oral composition are remarkably enhanced, and the present invention has been completed.

The present invention will be described in more detail below. The oral composition of the present invention comprises dextranase, alkyloyl sarcosinate and triclosan.

Here, as the dextranase, known ones can be used, and the compounding amount thereof is usually
100 to 50,000 units per 1 g of the oral composition,
More preferably, it is 200 to 20,000 units. Here, 1 unit means the amount of enzyme that produces a free reducing sugar corresponding to 1 μg of glucose per minute when the reaction is carried out using dextran as a substrate.

As the alkyloyl sarcosinate, lauroyl sarcosinate, myristoyl sarcosinate, palmitoyl sarcosinate and the like can be used, and their alkali metal salts such as Na salts are used. The blending amount is preferably 0.01 to 3% of the total amount of the composition, and more preferably 0.05 to 0.5%.
Is.

The oral composition of the present invention is a combination of dextranase and alkyloyl sarcosinate with triclosan, which gives an excellent plaque inhibitory effect and stability of dextranase. . In this case, other fungicides such as biozole, gluconic acid, chlorhexidine, chlorhexidine hydrochloride, benzethonium chloride, cetylpyridinium chloride, and benzalkonium chloride do not have sufficient effects and cannot achieve the object of the present invention.

The content of triclosan is 0.01-1% of the total amount of the composition, preferably 0.02-.
It is 0.5%. If the blending amount is less than 0.01%, the effect of suppressing plaque formation is not sufficient, and if it exceeds 1%, the taste of the composition remarkably deteriorates.

The composition for oral cavity of the present invention may contain appropriate components in addition to the above-mentioned active ingredients depending on its type. For example, in the case of toothpaste, an abrasive, a binder, a thickener, a surfactant, a sweetener, a fragrance, a coloring agent and the like can be added. In these cases, the conventionally used components can be used in a usual dose in each case.

[0011]

INDUSTRIAL APPLICABILITY The oral composition of the present invention has a high plaque formation-inhibiting effect and a high stability of dextranase.

[0012]

EXAMPLES The present invention will be specifically described below with reference to experimental examples and examples, but the present invention is not limited to the following examples.

[Experimental Example 1] The ability of dextranase, N-alkyloylsarcosine sodium and a fungicide to suppress plaque formation was examined by the following method. Experimental method (plaque formation inhibiting effect) Samples of the types and amounts shown in Table 1 were dissolved in 4 ml of 1% sucrose-added Blaine Heart Infusion medium, placed in a test tube, and anaerobically cultured Streptococcus. -After inoculating the mutans bacteria, it was anaerobically cultured for 18 hours in a gradient medium, and the amount of bacteria adhering to the test tube wall was measured. As a control, the same experiment was carried out using a medium to which no sample was added, and the amount of adherent bacteria (X _O ) at that time
Then, the plaque formation inhibition rate was obtained from the following equation from the amount of adherent bacteria (X _S ) when the sample solution was used. The results are shown in Table 1.

[0014]

[Equation 1]

[0015]

[Table 1]

From the results shown in Table 1, the composition containing triclosan in the oral composition using dextranase and sodium lauroyl sarcosine together has a high plaque formation inhibiting rate in the in vitro plaque formation inhibiting test. Indicated.

[Experimental Example 2] A toothpaste having the following composition was filled in a tube and stored at 40 ° C for 1 month. Then, the dextranase activity was measured by the following method, and the dextranase residual rate was calculated. The results are shown in Table 2. Toothpaste composition Aluminum hydroxide 45 (%) 60% Solbit 30 Carrageenan 0.8 Sodium alginate 0.3 Propylene glycol 3.0 Sodium lauryl sulfate 1.0 Lauric acid diethanolamide 1.0 Gelatin 0.3 Saccharin 0.1 Table Active ingredient of 3 Amount shown in Table 3 Fragrance 1.0 Purified water Residual 100.0%

Method for measuring the activity of dextranase 2 g of toothpaste is accurately weighed, 20 ml of water is added, and the mixture is shaken and stirred to extract dextranase. Further 3000
The supernatant liquid obtained by centrifugation at rpm for 10 minutes is used as an enzyme sample solution. 0.5 ml of the enzyme sample solution was previously stored at 35 ° C.
1% commercial dextran solution, which was kept at 35 ° C separately, that is, commercially available dextran in 0.1M phosphate buffer pH
1 ml of a 1% solution dissolved in 7.0 is added. After adding the dextran solution, reacting exactly at 35 ° C for 10 minutes, immediately adding 3,5-dinitrosalicylic acid to stop the reaction, and measuring the resulting reducing power by the 3,5-dinitrosalicylic acid method, The enzymatic activity of dextranase is calculated by converting the amount of reducing sugar into the amount of glucose. Specifically, 3 ml of a solution prepared by the 3,5-dinitrosalicylic acid method is added to stop the reaction, heated in boiling water for 10 minutes to complete the reaction, and cooled to 20 ml with water. The color development was measured by the absorbance at 530 mμ, and the reducing power was converted to glucose from the calibration curve. Regarding the residual enzyme activity of dextranase, the activity of dextranase immediately after the production of each toothpaste was defined as 100, and the dextranase activity after storage at 40 ° C. for 1 month was shown in percentage.

[0019]

[Table 2]

[Example 1] Toothpaste Aluminum hydroxide 45% by weight Sorbit 30 Sodium lauryl sulfate 0.8 Sodium alginate 0.6 Sodium saccharin 0.1 Gelatin 0.2 Diethanolamide lauric acid 1.6 Propylene glycol 5 Perfume 1. 3 Lauroyl sarcosine sodium 0.4 Triclosan 0.02 Dextranase 2000u / g Toothpaste Sodium monofluorophosphate 0.5 Water Residual 100.0% by weight

Example 2 Toothpaste Calcium carbonate 45% by weight Sorbit 24 Sodium lauryl sulfate 1.3 Carrageenan 0.7 Sodium alginate 0.3 Sodium saccharin 0.1 Gelatin 0.2 Diethanolamide laurate 0.8 Propylene glycol 4 Fragrance 1.1 Lauroyl sarcosine sodium 0.4 Triclosan 0.05 Dextranase 3000u / g Toothpaste Sodium monofluorophosphate 0.5 Water Residual 100.0% by weight

Example 3 Toothpaste Dicalcium Phosphate Dihydrate 50% by Weight Sodium Lauryl Sulfate 1 Carrageenan 0.6 Sodium Alginate 0.3 Sodium Saccharin 0.1 Gelatin 0.2 Diethanolamide Laurate 1 Propylene Glycol 4 Fragrance 1.1 Lauroyl sarcosine sodium 0.4 Triclosan 0.05 Sodium monofluorophosphate 0.5 Glycerin 20 Dextranase 3000u / g Toothpaste residual amount 100.0% by weight

[Example 4] Toothpaste Silica 20% by weight Sorbit 60 Sodium lauryl sulfate 0.9 Sodium saccharin 0.15 Gelatin 0.3 Myristic acid diethanolamide 1.5 Propylene glycol 5 Perfume 1.5 Lauroyl sarcosine sodium 0. 5 Triclosan 0.02 Sodium fluoride 0.2 Xanthan gum 0.5 Polyethylene glycol # 4000 0.5 Dextranase 5000u / g Toothpaste Residual 100.0% by weight

[Example 5] Toothpaste Modified aluminum hydroxide 50% by weight Sodium lauryl sulfate 1.5 Carrageenan 0.5 Sodium saccharin 0.14 Propylene glycol 5 Perfume 1.3 Myristoyl sarcosine sodium 0.3 Triclosan 0.5 Sodium monofluorophosphate 0.5 Glycerin 20 Silica anhydride 5 Titanium oxide 0.5 Sodium carboxymethyl cellulose 0.1 Dextranase 5000u / g Toothpaste Residual 100.0% by weight

Example 6 Liquid Toothpaste Silica 18% by weight Sorbit 55 Sodium lauryl sulfate 1 Sodium saccharin 0.1 Gelatin 0.2 Lauric acid diethanolamide 1.2 Propylene glycol 2 Perfume 1.4 Palmitoyl sarcosine sodium 0.4 Triclosan 0.3 Sodium fluoride 0.2 Glycerin 18 Xanthan gum 0.1 Decaglyceryl laurate 0.5 Sodium sulphate (anhydrous) 0.3 Dextranase 3000u / g Toothpaste Residual 100.0% by weight

[Example 7] Mouthwash Denatured ethanol 1.6 wt% Polyoxyethylene hydrogenated castor oil 2 Citric acid 0.01 Trisodium citrate 0.3 Perfume 0.5 Lauroyl sarcosine sodium 0.1 Triclosan 0 .02 sodium fluoride 0.08 glycerin 10 DL-alanine 1 dextranase 4600 u / ml mouthwash Leftovers Total 100.0% by weight

Example 8 Mouthwash 90% Ethanol 20% by weight Polyoxyethylene (80 mol) Sorbitan monotaurate 0.5 Perfume 1.5 Sodium monofluorophosphate 0.15 Triclosan 0.05 Lauroyl sarcosine sodium 0.1 Dextranase 4600u / ml mouthwash Leftovers Total 100.0% by weight

[Example 9] troche gum arabic 6% by weight flavor 1 sodium monofluorophosphate 0.05 triclosan 0.01 lauroylsarcosine sodium 0.01 glucose 36 palatinose 36 dextranase 4600u / g troche Leftovers Total 100.0% by weight

[Example 10] Chewing gum Gum base 20% by weight Flavor 1 Sodium monofluorophosphate 0.1 Triclosan 0.01 Lauroyl sarcosine sodium 0.01 Calcium carbonate 2 starch syrup 15 Powdered sugar 58 Dextranase 2000u / g gum water Residual 100.0% by weight

It was confirmed that each of the oral compositions of the above Examples can stably hold dextranase.

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[Procedure amendment]

[Submission date] February 9, 1994

[Procedure Amendment 1]

[Document name to be amended] Statement

[Correction target item name] 0008

[Correction method] Change

[Correction content]

The oral composition of the present invention is a combination of dextranase and alkyloyl sarcosinate with triclosan, which gives an excellent plaque inhibitory effect and stability of dextranase. . In this case, other fungicides such as biosol, chlor gluconate
Hexidine , chlorhexidine hydrochloride, benzethonium chloride, cetylpyridinium chloride, benzalkonium chloride and the like do not have sufficient effects and cannot achieve the object of the present invention.

[Procedure Amendment 2]

[Document name to be amended] Statement

[Correction target item name] 0019

[Correction method] Change

[Correction content]

[0019]

[Table 2]

Claims (1)

[Claims] 1. An oral composition comprising a combination of dextranase and alkyloyl sarcosinate, which further comprises triclosan.