JPH07112937A - Preventive and therapeutic agent for obesity from rice - Google Patents

Abstract

PURPOSE:To obtain a preventive and therapeutic agent for obesity from safe and inexpensive rice being completely safe even by habitual use. CONSTITUTION:This preventive and therapeutic agent for obesity contains either one of the followings (1)-(5) as it is: (1) a ground material of germinated rice, (2) an extract of rice or germinated rice, (3) a material obtained by decomposing a hydrolyzate of rice or germinated rice with an enzyme or treating the hydrolyzate with KOJI, (4) a material obtained, in extracting rice or germinated rice, by decomposing the rice or germinated rice with an enzyme or treating with Aspergillus oryzal (KOJI) before, during or after the extraction or (5) a material obtained by subjecting the extract of the rice or germinated rice, the decomposed material with an enzyme or the treated material with KOJI to alcohol fermentation or organic acid fermentation.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a prophylactic / therapeutic agent for obesity, which can be obtained from rice or sprouted rice as a raw material and can be used in the fields of medicine, food and the like.

[0002]

2. Description of the Related Art Today's Japanese food culture has become westernized, and it has become possible to easily eat food in a large number of countries. But,
On the other hand, new problems such as adult diseases have come to the surface, and "obesity" is mentioned as the cause. Then, various drugs for preventing obesity have been developed. However, these drugs have problems of side effects due to administration and restrictions on the amount used and the period of use. In addition, since these are mostly due to the mixture of the single substances, there is a problem from the side effect of the single substance and also from the viewpoint of the safety caused by long-term administration. That is, the present situation is that a preventive / therapeutic agent for obesity, which is effective for preventing / treating obesity, has no side effects, and is safe, has not yet been developed.

On the other hand, rice has been used as a staple food, as well as sake, shochu, mirin, vinegar, koji, etc., and has long been indispensable for daily life. In addition to this, bran bags are known for cosmetic purposes. It is believed that these were due to the fact that rice was viewed as merely a staple food, or at best as a source of starch. Also, even if the bran bag is said to be good for the skin, it has been customarily used as it is, and there was no concept of an active ingredient, nor was there any idea of utilizing that active ingredient.

[0004]

At present, there is a problem of side effects of drugs on the human body, and there is a demand for an obesity preventive / therapeutic agent that has no side effects and is sufficiently safe even if it is regularly used as a preventive / therapeutic agent. Has been done. An object of the present invention is to provide an agent for preventing and / or treating obesity from rice, which is safe, inexpensive, and completely safe even if it is used regularly.

[0005]

[Means for Solving the Problems] From the viewpoint of animal and plant harmony, the present inventors have conducted research on various plant components centering on rice, which is a staple food. In the process, it has become clear that rice has a number of potential and benefits that were previously unpredictable. Therefore, we picked up rice, which is used as a staple food and proved to have the highest safety, as the theme,
I have conducted comprehensive utilization research on rice. As one of the themes, we have been earnestly researching agents for preventing and / or treating obesity from rice, and in the process, rice and germinated rice contain a component having an anti-obesity effect. This has led to the completion of the present invention.

In the present invention, the components having anti-obesity action contained in rice and germinated rice have not yet been elucidated, but rice and germinated rice treated as described below , Oral administration revealed that it showed anti-obesity effect. Sprouted crushed rice as it is or containing it. Rice or germinated rice extract as it is or containing it. Enzyme-decomposed or hydrolyzed rice hydrolyzed as it is, or containing it. When extracting rice or sprouted rice, the one that has been subjected to enzymatic decomposition or koji before or at the same time as or after the extraction is used as it is or containing it. An extract of rice or germinated rice or a product of enzymatic decomposition or koji which has been subjected to alcohol fermentation or organic acid fermentation as it is or containing it.

The rice used in the present invention is Japonica,
Regardless of indica rice, it refers to non-glutinous rice, brown rice such as sticky rice, and white rice, regardless of variety and type. Further, 92% or more of red rice bran or 92% or less of white rice bran, which appears during whitening, may be used, which is inexpensive and economical. Also,
Germinated rice is used. In addition, since the active ingredient is stable to heat and light, the above raw materials are dipping, steaming, roasting (all sand roasting, net roasting, hot air roasting, etc.),
The raw material treatment such as steam roasting, surface modification such as freeze-drying, photo-modification such as UV irradiation, pressure roasting such as Patrice, and frying may be performed, and the effect was not changed.

Although the rice and germinated rice are effective as they are, they are preferably crushed and used from the viewpoint of practical use. In order to pulverize the rice and the sprouted rice into powder, it may be carried out by a general method using a pulverizer or a rice mill. When sprouting rice, germinated rice is soaked in water or sprayed with water to germinate. The temperature for germination is 5 to 70 ° C. However, the temperature and time do not matter as long as they germinate. Further, when there is a risk of water spoiling during germination, it is preferable to replace the water so that it does not spoil, or to perform some kind of preservative. Here, germination refers to everything from just before germination to germinated ones. The germinated rice is washed well before use. At this time, it may be dried before use.

When rice or sprouted rice is extracted or subjected to enzymatic decomposition or koji, if the raw material rice is crushed into granules or powder, the surface area is increased and the efficiency is improved. You don't have to grind, but in this case,
It takes a long time to decompose and extract the rice tissue. When extracting rice or germinated rice with water, a high extraction temperature is effective, but sufficient extraction can be performed even at a low temperature.
However, in the case of a low temperature of 40 ° C. or lower, it is desirable to make PH acidic or alkaline, or add a preservative or alcohol to treat the rice so that it does not spoil.
The extraction time may be long or short as long as the active ingredient can be extracted, and may be determined depending on the extraction temperature. The extraction may be carried out under pressure, at normal pressure, or under reduced pressure.

In the case of water extraction, the most problematic is the gelatinization phenomenon. If it becomes pasty, not only the extraction efficiency will deteriorate, but it will be extremely difficult in actual work. In order to prevent this, the reaction may be carried out by adding amylase or acidification may be carried out with hydrochloric acid or the like to cut the starch. By using this method, it can be sufficiently solved and there is no problem in practice.
Since the active ingredient in the extract is stable to acid and alkali, it is also effective to perform acid decomposition extraction or alkali decomposition extraction. In this case, neutralization and desalting are performed if necessary.

Also when extracting with an organic solvent, it is desirable to pulverize or pulverize rice as much as possible before extracting. The organic solvent may be a general organic solvent such as alcohol, acetone, n-hexane and methanol, but those harmful to the human body are preferably safe because it is necessary to completely remove the solvent after extraction. When enzymatically decomposing rice or sprouted rice, first add water to the rice or sprouted rice, then
Add enzyme. The amount of water added is appropriately selected depending on the yield, workability, purpose of final use, and the like. The optimum water temperature is the optimum temperature of the enzyme or koji, but enzymatic decomposition is sufficiently carried out even at low temperatures for a long time. However, if the temperature is lower than 40 ° C., some kind of preservative is required. The temperature may be high as long as it is decomposed. The decomposition time depends on the temperature and the like, but may be short or long as long as the decomposition is performed.

The enzyme used here is one or more of starch degrading enzyme, proteolytic enzyme, lipolytic enzyme, fiber degrading enzyme, lignin degrading enzyme and pectin degrading enzyme. When koji is used, the amount of water added, the temperature of action and the time of action are the same as in the case of enzymatic decomposition. The koji to be used may be generally used koji, and the type and variety of koji mold are not limited. Furthermore, in carrying out the above-mentioned extraction, the above-mentioned enzymatic decomposition and koji may be allowed to act before, simultaneously with or after the extraction. Here, when enzymatic decomposition or koji is allowed to act simultaneously with extraction, specifically, enzymatic decomposition or koji is allowed to act in an organic solvent, or enzymatic decomposition or koji is allowed to act under reduced pressure extraction.

In the present invention, alcohol fermentation or lactic acid fermentation is carried out at the same time as or after each of the above treatments,
It is more effective if organic acid fermentation such as acetic acid fermentation is performed. When carrying out this alcoholic fermentation, the extract obtained as described above, the enzymatic decomposition product (including those obtained by combining enzymatic decomposition and extraction) or the one obtained by allowing koji to act as it is, or after pressing and filtering The obtained liquid is subjected to alcohol fermentation. In addition, enzymatic decomposition and alcohol fermentation may be performed simultaneously. That is, after adding water to rice or sprouted rice, add enzyme or koji, and then sake mother or yeast,
Perform saccharification and alcohol fermentation. If necessary, alcohol may be fermented by supplementing sugar. When producing in large quantities, considering the balance between saccharification and fermentation, it is desirable to add rice or sprouted rice in three or more stages according to sake brewing. Especially when treating a small amount, it is effective to add them all at once. Saccharification and alcoholic fermentation are carried out for 10 to 24 days. At this time, when decay is a concern, acid is added or suitable preservative that does not hinder fermentation is applied.

Alcohol fermentation also has the advantage that concentration is easy and the active ingredient is easily concentrated. When performing lactic acid fermentation, it is the same as alcohol fermentation,
In this case, lactic acid fermentation is performed by adding lactic acid bacteria instead of liquor or yeast. Lactic acid fermentation is carried out by a general ordinary method, and the type of lactic acid bacterium and the conditions for lactic acid fermentation do not matter. Next, in the case of acetic acid fermentation, the fermented product obtained as described above is used as it is, or after being diluted to 4-5% of alcohol, acetic acid bacteria are added to perform acetic acid fermentation. If alcohol is not used, acetic acid fermentation is performed by adding alcohol. Acetic acid fermentation is carried out by a general ordinary method, and the type of acetic acid bacterium and the conditions of acetic acid fermentation are not limited.

The product of the present invention obtained as described above is used as it is without separating the residue, or after being pressed and filtered. When used as it is, it should be sterilized or sterilized before use. In addition, you may dry and use it as a powder, granules, a tablet, etc. Furthermore, it can be used by being mixed with various foods. The effect of the anti-obesity agent of the present invention will be described below based on the test.

Three-week-old male ddY mice were administered with the product of the present invention in an amount of 0.2 ml every other day, and one was administered with water as a control. Then, the animals were kept in the animal laboratory for 15 weeks. The number of samples was 15 each, and blood was collected every 5 weeks, serum was collected, and the components in the blood were analyzed. The body weight was also measured every 5 weeks.

Measurement of Free Fatty Acids Free fatty acids were quantified using the Itaya-Ui method. The method was to prepare three test tubes with stoppers, one for serum (plasma) 0.3 ml, the other 0.5 mM palmitic acid standard solution 0.3 ml, and the other one blind. For testing, 0.3 ml of water is added, 2 ml of phosphate buffer and 6 ml of chloroform are added to each, and the mixture is vigorously shaken for 90 seconds, and further shaken for 60 seconds. After resting for 15 minutes or more, the upper layer is suctioned off with a Pasteur pipette. Add 3 ml of copper reagent, stopper and shake up and down 30 times. After standing for 15 minutes or more, the copper reagent layer is suctioned off with a Pasteur pipette. 0.25 ml of a coloring reagent was added to the remaining chloroform layer to develop color, and the absorbance was measured at 440 nm using a blind test as a control, and the serum FFA concentration was calculated based on the measured value. The results are shown in Table 1.

[0018]

[Table 1]

From the above results, it can be said that the rice extract has activated fatty acid metabolism and changed into a constitution in which fat does not easily accumulate in the body. Next, the total lipid content [Total lipid (mg / dl)] and triglyceride [Triglyceride (mg / d)]
l)], HDL-cholesterol [HDL-chole
sterol (mg / dl)] was measured. The results are shown in Table 2.
Shown in.

[0020]

[Table 2]

From the above results, the total lipid was greatly reduced as compared with the control group. Moreover, since the amount of triglyceride is decreasing, it can be seen that the decomposition of fat, which is bad for the body, is progressing. And HDL-c
Since cholesterol, which is good for the body called “holesterol”, is increasing, it can be said that it is a very beneficial extract for the living body. Next, Table 3 shows the weight of each mouse.

[0022]

[Table 3]

From the above results, a decrease in body weight of about 20% is seen as compared with the control group, but from the above-mentioned results, there is no problem in the living body, rather the fat content is decreased, and Can be said to be healthy. Therefore, the product of the present invention can be said to be a very excellent antiobesity agent. From the above results, it can be seen that the average ulcer coefficient of physiological saline is 27.2, while it is clearly effective in all the products of the present invention according to the examples.

[0024]

Example 1 1 kg of rice with an embryo attached was immersed in water at 25 ° C. and immersed for 3 days to germinate the rice. After thoroughly washing the germinated rice, it is dried at 50 ° C. for 24 hours, and then,
The product was finely pulverized to obtain 990 g of the product of the present invention. (Example 2) Pulverized rice is crushed to obtain a pulverized product of white rice 500
g was obtained. 1500 ml of water was added to this pulverized product, PH was dropped with hydrochloric acid, and the mixture was left for 10 days. Then, it was squeezed with a squeezing machine to neutralize the resulting clear liquid to obtain 1200 ml of the product of the present invention and 760 g of a residue.

Example 3 Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 3 was carried out to obtain another 1190 ml of the product of the present invention. (Example 4) Pulverized rice is crushed to obtain a pulverized product of white rice 500
g was obtained. Liquefaction enzyme 10g and water 1500m to this crushed material
1 was added. Then, the temperature was gradually raised, and the mixture was boiled and extracted for 5 minutes and then cooled. Then, it was squeezed with a squeezing machine to obtain 1420 ml of the product of the present invention and 560 g of a residue.

Example 5 Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 4 was carried out to obtain 1400 ml of another product of the present invention. (Example 6) Pulverized rice is crushed to obtain 500 pieces of crushed white rice.
g was obtained. 1500 ml of 2N-NaOH was added to this pulverized product and the mixture was left for 5 days. Then, it was squeezed with a squeezing machine to obtain 1350 ml of the clear liquid and 650 g of the residue. 1 of this clarified liquid
Neutralization with 0N-HCl gave 1480 ml of the product of the present invention.

Example 7 Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 6 was carried out to obtain another 1490 ml of the product of the present invention. (Example 8) Pulverized rice was crushed to obtain 500 pulverized products of white rice.
g was obtained. 1500 ml of 95% ethanol is added to this pulverized product.
Was added and left for 5 days. After that, squeeze with a wringer,
1300 ml of clear liquid and 650 g of residue were obtained. 2000 ml of water was added to this clarified solution, and the mixture was concentrated by a rotary evaporator to obtain 1500 ml of the product of the present invention.

Example 9 Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 8 was carried out to obtain another 1500 ml of the product of the present invention. (Example 10) Crushed white rice into a crusher, and crushed white rice 50
0 g was obtained. 300 g of koji and 1500 ml of water
Was added and the mixture was allowed to stand at 55 ° C. for 20 hours. Then, it was squeezed with a squeezing machine to obtain 1230 ml of the product of the present invention and 1000 g of a residue.

(Example 11) Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 10 was carried out,
1210 ml of another product of the present invention was obtained. (Example 12) Crushed white rice into a crusher, and crushed white rice 50
0 g was obtained. 2 g of protease and 150 water
0 ml was added and the mixture was left at 50 ° C. for 20 hours. Then, the product was squeezed with a squeezing machine to obtain 1310 ml of the product of the present invention and 670 g of a residue.

(Example 13) The same operation as in Example 12 was carried out using 500 g of the product of the present invention obtained in Example 1,
Another 1380 ml of the product of the present invention was obtained. (Example 14) Pulverized rice was crushed to obtain a pulverized product of white rice 50
0 g was obtained. 2 g of lipolytic enzyme and 150 water
0 ml was added and the mixture was left at 50 ° C. for 20 hours. Then, it was squeezed with a squeezing machine to obtain 1290 ml of the product of the present invention and 680 g of a residue.

Example 15 Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 14 was carried out,
Another 1360 ml of the product of the present invention was obtained. (Example 16) Pulverized rice is crushed to obtain a pulverized product of white rice 50
0 g was obtained. 2 g of fiber-degrading enzyme and 150 water
0 ml was added and the mixture was left at 50 ° C. for 20 hours. Then, the product was squeezed with a squeezing machine to obtain 1330 ml of the product of the present invention and 650 g of a residue.

Example 17 Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 16 was carried out,
Another 1370 ml of the product of the present invention was obtained. (Example 18) Pulverized rice was crushed to obtain 50 crushed pieces of white rice.
0 g was obtained. 2g starch degrading enzyme and 150g water
0 ml was added and the mixture was left at 55 ° C. for 20 hours. Then, it was squeezed with a squeezing machine to obtain 1380 ml of the product of the present invention and 600 g of a residue.

Example 19 The same operation as in Example 18 was carried out using 500 g of the product of the present invention obtained in Example 1,
Another 1400 ml of the product of the present invention was obtained. (Example 20) White rice was crushed to obtain 50 pieces of crushed white rice.
0 g was obtained. Add 2 g of pectin-degrading enzyme and 1 part of water to this ground product.
500 ml was added and left at 50 ° C. for 20 hours. After that, squeezing with a squeezing machine, 1320 ml of the present invention product and residue 660
g was obtained.

Example 21 Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 20 was carried out,
Another 1300 ml of the product of the present invention was obtained. (Example 22) Crushed white rice into a crusher, and crushed white rice 50
0 g was obtained. To this pulverized product, 2 g of proteolytic enzyme, 2 g of lipolytic enzyme, 2 g of fiber degrading enzyme, 2 g of starch degrading enzyme, 2 g of pectin degrading enzyme and 1500 ml of water were added, and the mixture was heated at 50 ° C. for 2 hours.
It was left for 0 hours. After that, the product of the present invention 14
20 ml and 560 g of residue were obtained.

(Example 23) Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 22 was carried out,
Another 1440 ml of the product of the present invention was obtained. (Example 24) The same operation as in Example 22 was carried out to obtain 2000 g of an enzymatic decomposition product of white rice. Then, the temperature was gradually raised, and the mixture was boiled and extracted for 5 minutes and then cooled. Then, it was squeezed with a squeezing machine to obtain 1400 ml of the product of the present invention and 550 g of a residue.

(Example 25) The same operation as in Example 24 was carried out using 500 g of the product of the present invention obtained in Example 1,
1420 ml of another product of the present invention was obtained. (Example 26) Pulverized rice was crushed to obtain a pulverized product of white rice 50
0 g was obtained. To this crushed product, 300 g of koji and 1500 ml of 40% ethanol were added, and the mixture was left at 55 ° C. for 48 hours. After that, squeeze with a squeezing machine and clarified liquid 1300 ml and residue 850
g was obtained. Then, 1000 ml of water was added to the clarified liquid, and the mixture was concentrated by a rotary evaporator to obtain the product 13 of the present invention.
00 ml was obtained.

(Example 27) The same operation as in Example 26 was carried out using 500 g of the product of the present invention obtained in Example 1,
Another 1300 ml of the product of the present invention was obtained. (Example 28) White rice extract 2 was prepared in the same manner as in Example 4.
000 g was obtained. To this extract, 2 g of proteolytic enzyme, 2 g of lipolytic enzyme, 2 g of fiber degrading enzyme, 2 g of starch degrading enzyme,
2 g of pectin degrading enzyme was added, and the mixture was left at 50 ° C. for 24 hours. Then, it was squeezed with a squeezing machine to obtain 1400 ml of the product of the present invention and 580 g of a residue.

Example 29 The same operation as in Example 28 was carried out using 500 g of the product of the present invention obtained in Example 1,
Another 1390 ml of the product of the present invention was obtained. (Example 30) In the same manner as in Example 24, 2000 g of an enzyme-decomposed extract of white rice was obtained. Yeast was added to this enzyme-decomposed extract, and alcoholic fermentation was carried out for 16 days. Then, it was squeezed with a squeezing machine to obtain 1880 ml of the product of the present invention and 80 g of a residue.

(Example 31) The same operation as in Example 30 was carried out using 500 g of the product of the present invention obtained in Example 1,
Another 1800 ml of the product of the present invention was obtained. (Example 32) In the same manner as in Example 24, 2000 g of an enzyme-decomposed extract of white rice was obtained. The enzyme-decomposed extract was sterilized by boiling, cooled to 37 ° C., 200 ml of a starter in which lactic acid bacteria had been cultured in advance was added, and the mixture was well stirred and sealed,
Lactic acid fermentation was carried out at 37 ° C for 2 days. Then, it was squeezed with a squeezing machine to obtain 1380 ml of the product of the present invention and 590 g of a residue.

(Example 33) Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 32 was carried out,
Another 1400 ml of the product of the present invention was obtained. (Example 34) The product 1000 of the present invention obtained in Example 24.
80 ml of 95% ethanol was added to ml, and acetic acid fermentation was performed for 20 days. Then, it is filtered and the product of the present invention 990 m
1 was obtained.

(Example 35) Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 34 was carried out,
Another 1000 ml of the product of the present invention was obtained. Examples of blending the product of the present invention into a tablet and a soft drink will be described below. The formulation examples are not limited to the examples below.

(Example 36) Tablets 100 g of the product of the present invention obtained in Example 24 was dried by freeze drying to obtain 20 g of a dried product. This dried product 1
Tablets were obtained from 0 g as described below. Product of the present invention 10 g Polyethylene glycol 6000 10 g Sodium lauryl sulfate 1.5 g Corn starch 3 g Lactose 25 g Magnesium stearate 0.5 g

After weighing the above components, polyethylene glycol 6000 is heated to 70 to 80 ° C., the product of the present invention, sodium lauryl sulfate, corn starch and lactose are added and mixed, and then cooled as it is. The solidified mixture is crushed by a grinder. The granules are mixed with magnesium stearate and compressed to give tablets having a weight of 250 mg.

(Example 37) Soft drink The product of the present invention obtained in Example 22 15% (weight ratio) Licorice extract 0.01% Sugar 4% Lemon juice 2.5% Purified water 78.49% By a conventional method After mixing and stirring, soft drink was obtained.

[0045]

INDUSTRIAL APPLICABILITY According to the present invention, by continuously eating and drinking, an excellent obesity preventive / therapeutic agent which is simple, completely safe, and has the effects of preventing and treating cancer can be obtained. Since rice has been the staple food until now, little has been developed about its manufacturing method and use in new fields other than food. Furthermore, rice has been the staple food until now, and its safety has been well demonstrated. That is, the present invention not only finds a very excellent obesity preventive / therapeutic agent, but also finds a new use application, which is said to be overproduction of rice, and aims to increase consumption by improving the image of rice. Is extremely meaningful.

Claims (5)

[Claims] 1. A prophylactic / therapeutic agent for obesity, which comprises a crushed product of sprouted rice as it is or contains it. 2. A prophylactic / therapeutic agent for obesity, which comprises rice or an extract of germinated rice as it is or containing the same. 3. A prophylactic / therapeutic agent for obesity, which is obtained by enzymatically decomposing rice or a hydrolyzed rice germinated product with koji, or containing the same. 4. Extraction of rice or sprouted rice, before or at the same time as or after extraction, which has been subjected to enzymatic decomposition or koji, as it is, or containing obesity to prevent obesity. Therapeutic agent. 5. Prevention or treatment of obesity, which is obtained by subjecting rice or germinated rice extract or enzyme-decomposed or malted rice to alcohol-fermentation or organic acid-fermentation as it is, or containing the same. Agent.