JPH07104328B2 - Sample capsule for gel electrophoresis and its use - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION (a) Field of Industrial Application The present invention relates to sample injection in gel electrophoresis.

(B) Conventional technology Conventionally, in gel electrophoresis, as a method of placing a sample on a gel, the sample is soaked in a filter paper,
This filter paper was placed on the gel, or the sample in the buffer solution on or near the gel was directly injected using a syringe or the like.

(C) Problems to be solved by the invention In the conventional sampling technology, the sharpness of the band formed when the sample is electrophoresed and separated by isoelectric point or molecular weight sieving is distorted, which has a great influence on the poor separation. Exerted

In addition, when handling serum proteins as a sample, the method of sampling using a syringe or the like requires careful operation and may cause secondary infection.

(D) Means for Solving the Problems The present invention is characterized by a sample capsule obtained by injecting a target sample to be separated / analyzed into the center of a gel capsule and freezing in such a state.

The gel-like capsule is made of agarose gel, acrylamide gel, etc. The diameter of the resulting capsule may be determined depending on the size and thickness of the gel to be electrophoresed, but it is about 2 to 3 mm.

The usage of the sample capsule of the present invention is as follows.

First, the gel solution is poured into the electrophoresis tube, and the sample capsule is put into the tip of the electrophoresis tube to gel before completely gelling. After completion of gelation, attach an electrode bath to the electrophoresis tube and perform electrophoresis.

(E) Action In the present invention, the sample is frozen together with the gel and stored and handled in that state.

(F) Example A configuration of the present invention will be described with reference to the drawings.

FIG. 1 shows a sectional view of a sample capsule according to the present invention. Reference numeral 1 is an agarose gel having a diameter of 2 to 3 mm, and a target sample (eg, serum protein) 2 is dropped into this center and injected. In addition, a pore is opened in the center of the agarose gel 1 and the sample is injected by the capillary phenomenon. Freeze in this state and save.

The frozen sample capsule is used by using the gel electrophoresis apparatus for sample capsule shown in FIG.

Reference numeral 3 in FIG. 2 is a migration tube, and its inner diameter must be large enough to accommodate the sample capsule S. As shown in FIG. 3, an electrophoresis gel solution (agarose gel solution) 3a for injection is injected into the electrophoresis tube 3 by a syringe 9 and gelled therein. In addition, 10 is an adapter for injection.

Before completely gelling, the sump capsule S is put into the electrophoresis tube 3 from above and gelled in the state shown in FIG.

After that, the electrode baths of 4,5 are attached, the buffer solution is put into each, and the electrodes of 6, 7 are connected to the power source of 8, and voltage is applied to perform electrophoresis.

However, the sample capsule S must be stored and handled in a frozen state because the sample is present in the center of the sample capsule S, and must prevent diffusion of the sample in the capsule.

In the above-mentioned examples, only a one-dimensional tube gel electrophoresis apparatus is described. However, since it is also used for a flat plate (slab) gel, it is not limited to one-dimensional electrophoresis but is applicable to all gel electrophoresis such as two-dimensional electrophoresis. You can When applied to a flat gel, it is preferable to use a gel housing structure having a structure such that the sample capsule exists in the electrophoresis gel.

(G) Effect According to the present invention, the sample is easily handled when the electrophoretic gel is injected, and a syringe or the like is not handled when sampling is performed on the electrophoretic gel.

In addition, since the sample exists in the electrophoretic gel, the sample does not migrate on the gel surface when it migrates, but always migrates in the gel, so that the conditions for forming the band of the separation pattern can be improved.

Furthermore, it is possible to facilitate the automatic injection of the sample and improve the reproducibility of the separation pattern by improving the reproducibility of the sampling position.

[Brief description of drawings]

FIG. 1 is a sectional view of a sample capsule, FIG. 2 is a gel electrophoresis apparatus for capsules, and FIG. 3 is a diagram when a gel is injected into a migration tube. 1 ... Gel capsule, 2 ... Sample

Claims (3)

[Claims] 1. A sample capsule for gel electrophoresis obtained by freezing a target sample to be separated and analyzed at the center of the gel capsule. 2. The sample capsule for gel electrophoresis according to claim 1, wherein the gel capsule is made of agarose gel or acrylamide gel. 3. A gel electrophoresis sample capsule obtained by freezing a target sample to be separated and analyzed in the center of a gel capsule before pouring the gel solution into the electrophoresis tube and completely gelling the gel solution. A method of using a sample capsule for gel electrophoresis, which is characterized in that it is placed at the tip of a tube and after the gelation in the electrophoresis tube is completed, an electrophoresis is performed by attaching an electrode tank to the electrophoresis tube.