JPH0699318B2 - Antidiabetic agent consisting of water-soluble extract from Toberaceae plant - Google Patents

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to a water-soluble extract which is effective for the treatment of diabetes from plants of the family Tiberaceae.

[0002]

2. Description of the Related Art Vitamin B12, which is also known as cobalamin, is an animal protein factor, which was once extracted from animals in extremely small amounts, and is now extracted from microorganisms. Also,
Demand for cobalamin is increasing.

[0003]

The present inventor has found that vitamin B12 is present in extracts from plants of the family Tiberaceae, and whether this extract is due to the presence of this vegetable vitamin B12 or other unidentified components. I don't know yet, but I found it very effective in treating diabetes.

Therefore, the present invention relates to a therapeutic agent for diabetes which comprises an extract from a plant of the family Tiberaceae. This extract contained 10 g of lyophilized powder when analyzed.
2.0 μg Vitamin B12, 9.5 mg Vitamin B2 per
It contains 2.5 g of tannin and other unanalyzed constituents, although the quantity is undetermined but also contains a small amount of saponin.

The extract of the present invention is a plant of the family Toberaceae, such as, for example, Pttosporum glabratum Lindl, Pittosporum glabratum Lindl, and Tobera Pittospor.
um Tobira Ait), Shimatobera (Pitto- sporum undulatum)
Vent) etc. by the following method: The roots, stems, leaves, etc. of Toberaceae plants are made into a dry powder and mixed with water--preferably several times as much as the powder--and allowed to stand for several days. When left to stand, it separates into two layers, an aqueous solution and a precipitate. The aqueous solution is concentrated under reduced pressure by evaporating water in a boiling state at a temperature of about 40 ° C close to body temperature. The solution thus concentrated is the solution containing the extract. Add this solution from -30 to -40
It can be freeze-dried to a temperature of ℃ and then powdered by a freeze-drying method in which it is dried by sublimation of water under a reduced pressure of 1 to 0.01 mmHg until it reaches a room temperature.

The powdery extract was dissolved in an equal amount of water, measured, and analyzed by infrared absorption spectrum. As a result, an infrared absorption spectrum as shown in FIG. 1 was obtained. That is, the spectrum shows characteristic absorption bands of the following: a large absorption band: 1050~1070cm ^-1, 1620cm ^-1, 2950cm
^-1 and 3450 cm ^-1 Relatively large absorption bands: 520 cm ^-1 , 780 cm ^-1 , 1250 cm
^-1 , 1320cm ^-1 , 1380-1440cm ^-1 doublet (trapezoid), 17
35 cm ^-1 and 2850 cm ^-1 Thus, extracts used in the present invention, by a dry powder of pittosporaceae plants to stand by mixing with water, separating the solution layer and precipitate layer two layers, except the precipitate layer The latter solution layer was obtained by concentrating under reduced pressure at a temperature as low as about 40 ° C. to form a concentrated solution and, if necessary, pulverizing by freeze-drying, and showed the following infrared absorption spectrum: large absorption band: 1050~1070cm ^-1, 1620cm ^-1, 2950cm
^-1 and 3450 cm ^-1 Relatively large absorption bands: 520 cm ^-1 , 780 cm ^-1 , 1250 cm
^-1 , 1320cm ^-1 , 1380-1440cm ^-1 doublet (trapezoid), 17
35 cm ^-1 and 2850 cm ^-1 and vitamin B12 as a main component, vitamin B2, saponin, consisting a water-soluble extract containing tannins, in diabetes treatment agent.

When the above extract is used as such a therapeutic agent, it can be used as it is or as a solution before freeze-drying after concentration under reduced pressure, or it can be further freeze-dried. It is particularly advantageous to use it in the form of powder. Since the lyophilized product is also easily dissolved in water, it can be used by dissolving it in water, but the lyophilized powder can also be orally administered as a powder or tablet by mixing an excipient such as lactose.

For oral administration in the form of a solution, for example, an extract having a concentration of 50 to 600 mg / l is generally used in an amount of about 100 m.
Effective at 2 to 4 times per I / times. However, it is preferred that the freeze-dried powder as described above be orally administered as a 10 to 20-fold powder.

The acute toxicity of the above extract was extremely low, and it was found to be harmless within a physically acceptable range when measured with a 10-fold diluted powder by oral administration in rats.

[0010]

EXAMPLES The present invention will be described in more detail below with reference to examples. Example 1 Equally equal amounts of Plutosporum glabratum Lindl and Pittosporum glabratum Lindl are placed on the leaves for 6 months, and the roots and stems are shade-dried for 1 year before powdering. In the device shown in Fig. 2, 200 g
The powder of this is put into the inner container (2) composed of a net. In the container (1) placed outside this (2) container,
Introduce 1 liter of water to completely submerge the powder in water. In this state, keep the temperature at about 40 ℃ while heating. The heating time is 5 days. After this time, the outer container contained the aqueous layer (a) and the fine powder precipitate layer (b) from the container (2).
Is recognized. Coarse coarse sediment layer in the container of (2)
(c) remains. 850 obtained, except for layers (b) and (c)
Only ml of the aqueous layer (a) remains in the container. The concentration of this aqueous solution is 15 g / l. Vacuum pump this aqueous solution
Decompress under (3) and concentrate at a temperature of about 40 ° C to obtain a concentrated solution of 353 ml. The concentration of the concentrated extract solution is 36.5 g / l. The solution was snap frozen at -30 ° C and then allowed to reach room temperature within 3 hours at 0.1-0.3.
Maintain a reduced pressure of mmHg. 1.1 by this lyophilization
0 g of brown powder is obtained. This powder is freely soluble in water.

Example 2 (Test) To this powder was added twice the amount of water to dissolve it, and the infrared absorption spectrum of this was measured. The results are shown in Fig. 1.

The components contained in the above powdery extract were measured by various methods. The results and measurement methods are shown below: Vitamin B12 2.0 μg / 10g Microbiological method ^*) Vitamin B2 9.5mg / 10g Lumiflavin Fluorescence method Tannin 2.5g / 10g FOLIN-DENIS method *) Strains used: Lactobacillus leichmannii AT CC 7830 The other components remain unmeasured.

Example 3 (Use Example) The results of applying the extract of the present invention to diabetic patients and patients with liver diseases in the form of a solution prepared by diluting powder after freeze-drying with 10 times the amount of water are shown below. explain.

For the treatment of diabetic patients, the above diluted solution of the extract was administered at a dose of 1.5 g three times a day. The first patient was a 40-year-old woman with a blood glucose level of 166 mg / dl before administration, but 75
142 mg / dl after 90 days, 120 mg / dl after 90 days and 13
Normal value of 110 mg / dl after 0 days (normal value 60-110 mg / dl)
Fell to.

The second patient is a 52 year old female, 20 before administration.
The blood glucose level was 4 mg / dl, but after 60 days it was 166 mg / dl,
It dropped to a normal value of 140 mg / dl after 75 days and 106 mg / dl after 90 days.

As can be seen from the above-mentioned effects, the extract of the present invention has been found to be very effective in treating diabetes.

[0017]

INDUSTRIAL APPLICABILITY As explained above, the extract from the plants of the family Toberaceae contains vitamin B12, which was believed to be absent in the plant, and, surprisingly, is very excellent for the treatment of diabetes. Show medicinal effects.

[Brief description of drawings]

FIG. 1 is an example of an embodiment of the present invention.
2 is an infrared absorption spectrum of the powder extract obtained in 1.

FIG. 2 shows an extraction apparatus used in the extraction method described in Example 1. The symbols in FIG. 2 have the following meanings: 1 ... Outer container 2 ... Net inner container 3 ... Vacuum pump a ... Water-soluble layer b ... Fine powder precipitation layer c ... .Large sediment layer

Claims (1)

[Claims] 1. A dry powder of a Toberaceae plant is mixed with water and left to separate into two layers, a solution layer and a precipitation layer. After the precipitation layer is removed, the solution layer is heated at a low temperature of about 40 ° C. at the maximum. in obtained by powdered by lyophilization, if concentrated to a thick solution and necessary under reduced pressure, and yielded the following infrared absorption spectrum: a large absorption bands: 1050~1070cm ^-1, 1620cm ^-1, 2950cm
^-1 and 3450 cm ^-1 Relatively large absorption bands: 520 cm ^-1 , 780 cm ^-1 , 1250 cm
^-1 , 1320cm ^-1 , 1380-1440cm ^-1 doublet (trapezoid), 17
35 cm ^-1 and 2850 cm ^-1 and vitamin B12 as a main component, vitamin B2, saponin, diabetes及therapeutic agent consisting of a water-soluble extract containing tannins.