JPH0692862A - Improver for intestinal flora - Google Patents

Abstract

PURPOSE:To obtain an improver for intestinal floras capable of eliminating the malodor of a culture solution prepared from Clostridium butyricum or a filtrate thereof and manifesting effects of an active ingredient possessed by the culture solution of the live Clostridium butyricum or the filtrate thereof. CONSTITUTION:This improver for intestinal floras is obtained by blending a culture solution of a live bacterium for controlling the intestinal function represented by Clostridium butyricum with a deodorant and eliminating the malodor of the culture solution or a filtrate thereof. An aqueous extract solution of rice bran is preferred as the deodorant. This improver is an improver for the intestinal floras capable of more manifesting effects of the active ingredient possessed by the culture solution of the Clostridium butyricum or the filtrate thereof and effectively used for humans, livestock, poultries, fisheries animals.

Description

Detailed Description of the Invention

[0001]

The present invention relates to humans, livestock and poultry,
The present invention relates to an intestinal flora improving agent administered to aquatic animals.

[0002]

2. Description of the Related Art Conventionally, Clostridium butyricum has been
Since the live bacterium is useful as an intestinal regulator, it is used for humans, livestock / poultry, and aquatic animals. However, when Clostridium butyricum is used as a live bacterium, the detection rate from normal faeces is 30% when used in humans, the optimum growth temperature is approximately 35 ° C, and it is an anaerobic bacterium. Since the above conditions are required, there are cases in which the animal grows in the intestine of any animal such as humans, livestock / poultry, and aquatic animals, and the desired effects such as intestinal regulation are not always exhibited.

On the other hand, since the intestinal bioactive agent composed of live bacteria of Clostridium butyricum for the purpose of intestinal regulation has safety, it improves the various symptoms caused by abnormal intestinal flora, It is used for the purpose of increasing the weight of livestock, poultry and aquatic animals, and improving feed efficiency.
It is expected that more effective live bacteria of Clostridium butyricum will be improved.

[0004]

As described above, the live bacteria of Clostridium butyricum are used as an intestinal regulating agent and mixed with feed, and are controllable to bacteria harmful to humans, livestock / poultry and aquatic animals. It is also said that it works to promote useful bacteria and improves intestinal flora.

The live bacterium of Clostridium butyricum is an anaerobic spore-bearing bacterium, which is cultured in a normal agar medium containing 0.5% glucose at 37 ° C. for 48 hours, and the culture solution is observed under a microscope. , An almost linear or rarely slightly curved bacillus, forming oval-shaped spores. Also,
In the culture solution, in addition to live bacteria, butyric acid, which is a metabolite,
It contains organic acids such as acetic acid, and autolyzed cell components.

However, as described above, the culture filtrate remaining after the filtration of the culture solution of Clostridium butyricum to recover the viable cells contains the organic acid and the autolyzed cell components. It is effective for humans, livestock / poultry, and aquatic animals, but has a drawback of having a bad odor because it contains butyric acid as an organic acid. For example, butyric acid odor threshold (limit concentration that can distinguish the type of odor)
Is 4.3 × 10 according to Bessay
^As can be seen from the fact that it is ¹³ (number of molecules / cc), it is one of the odors that humans avoid. Therefore, the culture solution or culture filtrate of Clostridium butyricum is difficult to drink as it is, and when administered to livestock, poultry, and aquatic animals, its odor is unpleasant for workers,
The effective utilization was not considered yet, and only the viable bacteria isolated from the culture solution of Clostridium butyricum were used.

The present invention has been made in order to improve the above-mentioned drawbacks of the prior art, and eliminates the bad odor of the culture broth or the culture filtrate of the live bacterium of Clostridium butyricum and eliminates the odor. The effect of the active ingredient contained in the culture solution or the culture filtrate of live bacterium of D. butyricum was exhibited more effectively.
It is intended to provide an effective intestinal flora improving agent for humans, livestock / poultry, and aquatic animals.

[0008]

Means for Solving the Problems That is, the present invention contains, as an active ingredient, a deodorant culture filtrate in which a deodorant is added to a culture filtrate of a viable intestinal bacterium represented by Clostridium butyricum. It is an intestinal flora improving agent characterized by being

Further, the present invention is characterized in that the deodorant culture solution containing a deodorant in a culture solution of live intestinal bacterium represented by Clostridium butyricum is contained as an active ingredient. It is a flora improving agent.

The present invention will be described in detail below. The intestinal flora improving agent of the present invention is dissolved in a culture solution of live intestinal bacteria represented by Clostridium butyricum or a culture filtrate of live intestine bacteria represented by Clostridium butyricum. It contains a deodorant culture solution or a deodorant culture filtrate, which is deodorized by adding an odorant, as an active ingredient.

In the present invention, as the deodorant, for example, a water extract of rice bran, a sensory deodorant such as cyclodextrin, and a physical deodorant such as activated carbon are used. Of these, a water extract of rice bran is particularly preferable. The amount of the deodorant added is 1 to 30 with respect to 100 parts by weight of the culture solution or culture filtrate of Clostridium butyricum.
Parts by weight, preferably 5 to 20 parts by weight are desirable. If it is less than 1 part by weight, deodorization is not sufficiently performed, and even if it is used in excess of 30 parts by weight, the deodorizing effect does not change, and the amount of the deodorant increases, and the amount of the active ingredient contained in the culture solution or culture filtrate is increased. Diminishes the effect. However, the addition amount of the water extract of rice bran in the addition amount of the above deodorant indicates the addition amount as an anhydride.

A specific example of using a rice bran water extract as a deodorant is as follows: Clostridium butyricum was cultured in a normal broth medium containing 0.5% glucose at 37 ° C. for 48 hours to obtain a culture filter. To 100 parts by weight of the liquid, for example, as a deodorant, Table 1
5 to 40 parts by weight of an aqueous extract of rice sugar comprising the components shown in
The purpose of deodorization can be achieved by adding preferably 10 to 20 parts by weight, and more preferably about 12 parts by weight. The medium to be used is not limited to the normal agar medium supplemented with 0.5% glucose, but may be any medium such as milk that allows growth of Clostridium butyricum.

[0013]

[Table 1]

An intestinal flora improving agent consisting of a deodorant culture filtrate of an intestinal live bacterium of Clostridium butyricum or a deodorant culture broth containing a viable bacterium is usually used for humans, livestock / poultry and aquatic animals. It is desirable to administer 0.1 ml to 10 ml, preferably 1 ml to 4 ml per 1 kg of body weight per day.

The intestinal flora improving agent comprising a deodorant culture filtrate of a live bacterium of Clostridium butyricum or a deodorant culture containing a live bacterium may be administered as a liquid, or may be a carrier. It may be administered as a mixture with. Lactose, starch and the like are used as carriers, and they may be administered in the form of powder, granules or tablets.

[0016]

EXAMPLES The present invention will be described more specifically below with reference to examples. However, the present invention is not limited to these examples.

Example 1 Inhibitory action against bacteria harmful to humans, livestock / poultry and aquatic animals. Used strain Escherichia col
i), Salmonella Enteritidis
ella enteritidis), Candida albicans, Clostridium paraptophyticum (Clostrid)
ium Paraputrificam). Use medium 0.5% glucose-added broth agar medium.

Culture Method Escherichia col
i), Salmonella Enteritidis
ella enteritidis) and Candida albicans, aerobically cultivated at 37 ° C. for 24 hours, Clostridium palptophycum (Clostridium Pa)
rapeuticam) was anaerobically cultured at 37 ° C for 48 hours.

Test substance Agent A According to the method of the present invention. That is, 37% of normal broth medium containing 0.5% glucose of Clostridium butyricum
12 parts by weight of a water extract of rice sugar having the components shown in Table 1 as a deodorant was added to 100 parts by weight of a culture filtrate which was cultured at 48 ° C. for 48 hours. There was no odor in the culture filtrate after adding the deodorant. Agent B By the method used as a conventional intestinal regulating agent. That is,
A freeze-dried live bacterium obtained by centrifuging a culture solution obtained by culturing Clostridium butyricum in a normal broth medium containing 0.5% glucose at 37 ° C for 48 hours.

Assay method for inhibitory effect The strains used were 24 in normal broth medium containing 0.5% glucose.
After 0.1 ml of the time-cultured solution was added dropwise to the medium used and spread over one side, a penicillin assay cup was placed, agents A and B were added to the penicillin assay cup, and the cells were cultured according to the culture method for each bacterium, and the penicillin assay was performed. The width of the growth inhibition zone of the strain used, which was generated from the edge of the cup, was measured.

Measurement Results As shown in Table 2, the agent A of the present invention was used in Escherichia coli whose strain is aerobic.
coli), Salmonella enteritidis (Sal
monella enteritidis, Candida albican
s) as well as the anaerobic bacterium Clostridium Parapupu
However, the conventional agent B does not show a growth inhibitory effect on aerobic bacteria,
It showed a growth inhibitory effect only on anaerobic bacteria.

[0022]

[Table 2] (Note) +: Growth inhibition width is 3 mm or more-: Growth inhibition width is not seen

The results shown in Table 2 do not represent the state of the intestine as it is, but when the intestine is aerobic, viable bacteria of Clostridium butyricum cannot grow because they are anaerobic bacteria.
While showing that aerobic bacteria harmful to humans, livestock / poultry and aquatic animals may not be controlled in some cases, the intestinal flora improving agent of the present invention is harmful to humans, livestock / poultry and aquatic animals. It is observed to act on both aerobic and anaerobic bacteria.

Example 2 Effect on broiler A field application test was conducted to confirm the effect of administration of the intestinal flora improving agent of the present invention on broiler.

Method of Use The floor was warmed in a windless, flat-bed house for use management. The test categories were the A agent administration group and the B agent administration group, in which 0.1% by weight of A agent and 0.1% by weight of B agent were added to the commercial feed. In addition,
For the agent B, the viable cell count was measured upon addition, and the cell count was controlled so that it would always remain constant. The broiler used was the A agent administration group,
2,000 birds were administered to each of the B agent administration groups.

Implementation result

[0027]

[Table 3]

From the results shown in Table 3, the intestinal flora improving agent of the present invention tended to improve the number of shipped birds and the feed requirement rate as compared with the conventional agents. In addition, there were no complaints from workers regarding the difficulty of handling odors.

Example 3 In Examples 1 and 2, 100 parts by weight of a culture solution obtained by culturing 48 hours at 37 ° C. in a normal broth medium containing 0.5% glucose of Clostridium butyricum was used in place of the agent A. The same tests as in Examples 1 and 2 were performed, except that 12 parts by weight of a water extract of rice sugar having the components shown in Table 1 was used as the deodorant. The odor of the culture solution was deodorized, and the same results as in Examples 1 and 2 were obtained.

[0030]

As described above, according to the present invention, the bad odor of the culture solution or the culture filtrate of the viable bacteria of Clostridium butyricum is eliminated, and the viable bacteria of Clostridium butyricum is eliminated. It is possible to exert the effect of the active ingredient contained in the culture solution or culture filtrate of, human, livestock / poultry,
It is possible to provide an effective intestinal flora improving agent for aquatic animals.

Claims (6)

[Claims] 1. An intestinal flora containing, as an active ingredient, a deodorant culture filtrate in which a deodorant is mixed with a culture filtrate of a living intestinal bacterium represented by Clostridium butyricum. Improver. 2. An intestinal flora improving agent, which contains as an active ingredient a deodorant culture solution prepared by mixing a deodorant into a culture solution of a living intestinal bacterium represented by Clostridium butyricum. . 3. The deodorant is a water extract of rice bran.
Alternatively, the intestinal flora improving agent described in 2. 4. The intestinal flora improving agent according to claim 1 or 2, which is administered to humans. 5. The intestinal flora improving agent according to claim 1, which is administered to livestock, poultry or aquatic animals. 6. An intestinal flora improving agent obtained by mixing the deodorant culture filtrate or deodorant culture solution according to claim 1 or 2 with a carrier.