JP3009203B2 - Campylobacter Growth Inhibitors in Poultry or Livestock - Google Patents

Description

Description: TECHNICAL FIELD The present invention relates to an agent for suppressing the growth of Campylobacter in poultry or livestock.

(Prior art) Campylobacter grows in the intestine of poultry and livestock, and it becomes a pollutant for meat in slaughterhouses, and eating these meats often causes food poisoning. There is. For this reason, currently, the treatment plant usually carries out a disinfection process using a chlorine agent or the like.

(Problems to be Solved by the Invention) However, even if such disinfection is carried out, the limit of Campylobacter is reduced to about 1/10 or 1/100, and furthermore, the disinfection requires a huge cost. was there.

Accordingly, an object of the present invention is to provide an inhibitor of Campylobacter that grows in the intestine of poultry or livestock.

(Means for Solving the Problems) The above object is achieved by a growth inhibitor of Campylobacter in poultry or livestock containing Clostridium butyricum as an active ingredient.

(Action) As a typical example of Clostridium butyricum used in the present invention, there is Clostridium butyricum MIYAIRI, which is usually referred to as Miyairi bacteria (hereinafter abbreviated as Miyairi bacteria). This is an antiseptic butyric acid bacterium, which is a straight or slightly curved double-ended round-bottom bacillus, which forms an oval bean terminal spore. No side effect is observed even when the bacterium is administered to poultry for a long time. In addition, Miyairi has the effect of keeping the intestinal flora in equilibrium. That is, it has an antibacterial effect against coliform bacteria and Staphylococcus aureus, and has a growth promoting effect against beneficial bacteria such as lactic acid bacteria.

The germ entry usually depends on the age of the poultry, but usually weighs 1 kg.
The dose is 1 to 200 mg / day, preferably 3 to 20 mg / day. In addition, the bacterium is usually administered in the form of a mixture with a carrier. As a carrier, for example, there is a feed, and it is convenient to administer such a feed by using a mixture of bacteria. When blended into feed, the amount of Miyairi bacteria should be
The amount is 10 to 2,000 mg, preferably 20 to 50 mg, per kg of the sample.
Such feed components are, for example, those shown in Table 1.

In addition, various nutrients such as vitamins and minerals, and antibacterial feed additives such as antibiotics can be added to the feed.

The growth inhibitor of the present invention is useful for poultry such as chickens, turkeys, quails, and duck, and livestock in which Campylobacter is detected, for example, cattle, pigs, and sheep. It has excellent effect.

Next, the present invention will be described in more detail with reference to examples.

Examples (A) Test method (1) Test implementation period: The test was performed at A broiler farm for 29 days.

(2) Test chicken: Broiler (Arbor Acres / Fuji) 32 days old was used.

(3) Test product: Miyarisan preparation [Clostridium butyricu in 1 g of this product
m MIYAIRI588, (Microfabrication of Microorganisms No. 2,789), below: CBM58
8) containing 10 ⁷ or more spores (manufactured by Miyarisan Co., Ltd.)].

(4) Test category: The number of test birds was 7,300 each along with the control group and the administration group, and the feed of the administration group was supplemented with 0.1% of the Miyarisan preparation.

(5) Feed: For the feed, a commercially available broiler fat-free one containing no viable agent was used. The feeding period of each feed is from 32 days of age to late-stage feed ^* 22 days, and non-feeding feed 7 days.

^* Nosipeptide 2.5g titer / ton, colistin sulfate 5g titer / ton, lasalocid sodium 7.5g titer / ton (6) Feeding management: Flat dressing with windless floor heating (negative pressure ventilation) .

(7) Test items: Feeding test Weight gain, feed intake, and other information on intestinal campylobacter Taketo Ito et al. [: Campylobacter jejuni and Campylobact
eter coli test method, media circle, 30 , 182 (198
5)], morphology, catalase, oxidase, and aerobic culture were performed on the colonies generated in the selective medium (Butzler), and the colonies were separated and identified.

(B) Results (1) Feeding test Table 2 shows the results of the feeding test. The administration groups tended to have higher feed intake and shipping rates. The feed rate showed an improvement in the administration group. The production coefficient was 191 in the control plot.
In contrast to 2, it was 200.1 in the administration group, which was higher in the administration group.

(2) Campylobacter Changes in Campylobacter at the start of the test (32 days of age) and at the time of shipment (61 days of age) are shown in Table 3. Campylobacter was detected at relatively high levels of 10 ⁴ to 10 ⁵ / g and 10 ⁸ to 10 ⁹ / g from the ileum site and the cecal site, respectively, at the start of the test. During the administration period, the number of bacteria at each site increased significantly (p <0.05) in the control group at the ileum site, but almost no increase was observed in the administration group. Also,
At the cecum site, there was almost no change in the control group, but it decreased with a significant difference (p <0.05) in the administration group.

As is clear from the above examples, the changes in Campylobacter at the start of the test and after administration tend to decrease in the ileum and cecum by administration of CBM588 as compared to the control group. Contamination by Campylobacter of poultry meat varies depending on the situation of poultry processing plants, mainly are said to occur at the time of demolition, but the process always disinfection process is, here 1/10 ² about the number of bacteria It is said that the limit is lowered. Therefore, it is necessary to keep the number of contaminants such as Campylobacter and Salmonella in the broiler carried into the treatment plant at a low level. In other words, there is a demand for a broiler having a healthier and cleaner intestinal flora with less Campylobacter etc. on the treatment plant side.

In this study, CBM588 administration showed a tendency to reduce harmful bacteria and improved feeding performance.
There is great potential for the practical application of the purpose of this test for strengthening biological defense and suppressing food poisoning pathogens, especially Campylobacter.

(Effects of the Invention) As described above, the present invention is a Campylobacter growth inhibitor in poultry or livestock containing Clostridium butyricum as an active ingredient. There is an advantage that the growth of Campylobacter in the intestine is suppressed, and as a result, meat with low contamination by Campylobacter can be obtained at the time of dismantling.

Claims (4)

(57) [Claims] An agent for inhibiting the growth of Campylobacter in poultry or livestock comprising Clostridium butyricum as an active ingredient. 2. The growth inhibitor of Campylobacter according to claim 1, wherein the Clostridium butyricum is a mixture with a carrier. 3. The growth inhibitor of Campylobacter according to claim 2, wherein the carrier is a feed for poultry or livestock. 4. The growth inhibitor of Campylobacter according to any one of claims 1 to 3, wherein the poultry is a chicken.