JPH067918B2 - The chemical reaction device - Google Patents

The chemical reaction device

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JPH067918B2
JPH067918B2 JP21452186A JP21452186A JPH067918B2 JP H067918 B2 JPH067918 B2 JP H067918B2 JP 21452186 A JP21452186 A JP 21452186A JP 21452186 A JP21452186 A JP 21452186A JP H067918 B2 JPH067918 B2 JP H067918B2
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capillary
reagent
means
paragraph
capillaries
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JPS6369539A (en )
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英 持田
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持田製薬株式会社
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00178Special arrangements of analysers
    • G01N2035/00237Handling microquantities of analyte, e.g. microvalves, capillary networks
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04Details of the conveyor system
    • G01N2035/0401Sample carriers, cuvettes or reaction vessels
    • G01N2035/0406Individual bottles or tubes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04Details of the conveyor system
    • G01N2035/046General conveyor features
    • G01N2035/0465Loading or unloading the conveyor

Description

【発明の詳細な説明】 [産業上の利用分野] この発明は、例えばバイオテクロノジー、生化学等化学工業の分野に広く用いられるばかりでなく、微生物を含む生物化学の研究、あるいは臨床検査など多数の化学反応試験を行うのに適した化学反応装置に関するもので、 DETAILED DESCRIPTION OF THE INVENTION [Field of the Industrial] The present invention, for example biotechnology, not only widely used in the fields of biochemistry, such as chemical industry, research biochemical containing microorganisms, or laboratory tests such as relates chemical reaction apparatus suitable for performing multiple chemical reactions tested,
特に毛細管内で化学反応を行うことにより簡易かつ効率的に反応試験を行うことのできる化学反応装置に関するものである。 In particular it relates to a chemical reaction apparatus capable of performing simple and efficient reaction tested by performing a chemical reaction in the capillary.

[従来の技術] 多数のしかも量的には少量の試量を使用して化学反応を行なう方法および装置には、主として免疫化学反応を用いる臨床診断の領域において近来おおくの技術の進歩がある。 The [Prior Art] Numerous Moreover the quantitative method and apparatus for performing chemical reactions using small amounts of 試量, there are advances recently many techniques in the area of ​​clinical diagnosis using mainly immunochemical reactions. そのひとつとして小型のプラスチックなどの球形ビーズに免疫反応物質を固定化する方法などがある。 And a method of immobilizing immunoreactive material spherical beads, such as small plastic as one of them.

また発明者らが既に行なった特開昭61−114731および特開昭61−114732などがある。 Further, etc. JP 61-114731 and JP 61-114732, which we have already done. 上記発明における装置を使用するときに用いる反応容器は試験管を用い、しかもそれを傾斜させて回転させることによって反応時間を著しく短縮することができるもので従来の技術に比較して多数の検体を自動的に高速処理する目的に合致したものである。 Many samples as compared to the prior art in that it is possible to significantly shorten the reaction time by reactor using a test tube, moreover rotated by tilting it to be used when using the device in the invention it is obtained by matching the automatically fast processing purposes.

また免疫反応を行なうにあたって毛細管を使用する技術および装置もある。 There is also techniques and apparatus for using a capillary carrying out the immunological reaction. たとえば特表昭58−501521に記載されたものがある。 For example those described in Kohyo Sho 58-501521. しかしながらこの発明による装置は複数の毛細管を回転台の周囲に垂直に取りつけ、その毛細管で免疫反応を行なうものである。 However the apparatus according to the invention is mounted vertically a plurality of capillaries around the turntable, and performs an immune reaction in the capillary.

この装置によれば、毛細管内では反応が早いので攪拌装置が不要となり、装置が簡単かつ小型化される利点がある。 According to this device, since the early reactions in the capillary stirrer is not required, there is the advantage that the device is simple and compact.

また免疫反応のごとく試料として主に患者の血液を用いるものにあっては、一定量の血液から多数の項目の試験を行なわなければならないため、一項目あたりに使用できる血液の量が次第に少なくなっているが、毛細管内で反応を行う上記のものは旧来のものに比べて試料の量も少なくて済む長所を有している。 Also in regard to those used primarily for the patient's blood as a sample as the immune response, since it must perform the test of a large number of items from a given amount of blood, gradually reduces the amount of blood that can be used per item and that although the above that the reaction is carried out in the capillary has the advantage that only a small amount of sample as compared with the old.

[発明が解決しようとする問題点] 小型のプラスチックビーズを使用する方法はビーズそのものの表面に免疫反応物質を固定化する操作は大量生産に適しているが、このビーズを用いて実際に多数の免疫反応を行なう操作、たとえばビーズを試験管に投入する操作、ビーズを取り出して洗浄する操作、再びビーズを試験管に投入して試薬を添加する操作などが繁雑であり、それに適した自動化機構も種々のものが考案されているが複雑なメカニズムを採用しなければならないなどの欠点がある。 How to use plastic beads small [INVENTION Problems to be Solved] The operation of fixing the immunoreactive substance on the surface of the bead itself is suitable for mass production, but actually a number of using beads operation for the immune response, for example, an operation to inject beads into a test tube, an operation of washing the beads were removed, and the troublesome an operation of adding a reagent to reapply beads in a test tube, even automated mechanism suitable therefor Although various types have been devised there are drawbacks such as the need to adopt a complicated mechanism. また反応に要する時間が長いので高速の多数の検体処理には適さない。 Since a long time required for the reaction is not suitable for high speed multiple sample processing.

本発明者らの特開昭61−114731および特開昭61−114732 Japanese inventors of the present invention 61-114731 and JP-A-61-114732
などの発明による装置によればビーズの代わりに内面に免疫反応物質を固定化した試験管を用い、しかもこれを傾斜させて回転することによって反応時間を著しく短縮することができるので、ビーズを使用する方法に比べて多数の検体を高速自動処理する目的に最も適している。 Using tubes with immobilized immunoreactive substance on the inner surface, instead of the beads according to the device according to the invention, such as, moreover it is possible to significantly shorten the reaction time by rotating by inclining it, using beads It is best suited to the purpose of high-speed automatic processing many samples as compared to the method of.

しかしながら試験管の内面に免疫反応物質を固定化する操作がビーズの表面を使用する場合に比して著しく繁雑になり試薬の製造コストが高価となる。 However the production cost of the reagents becomes remarkably complicated in comparison with a case where the operation for fixing the immunoreactive substance on the inner surface of the tube to use the surface of the bead becomes expensive. すなわち試験管の場合その中に一定量の免疫反応物質の溶液を注入し一定時間放置後、内部の液を除去し、洗浄し乾燥する等の操作を必要とする。 That is, after injecting a predetermined time allowed a solution of a quantity of immunoreactive material when therein a test tube, to remove the liquid inside, requiring operations such as washing and drying. これに反しビーズを使用する場合たんに免疫反応物質の溶液中にビーズを多数投入すれば良く、つぎの洗浄乾燥などもきわめて容易で大量生産に適している。 May be many charged bead when solution of the immunoreactive substance in sputum of using beads contrary, is suitable for very easy mass production well as following cleaning and drying. すなわちビーズ法は臨床検査を行なう際に使用者には不便であるが製造は容易である。 That bead method is inconvenient manufacture the user in performing the lab test is easy. 試験管法は使用者には便利であるが製造が繁雑であるという逆の関係にある。 Test tube method is convenient to the user is in the reverse of the relationship that the production is complicated.

毛細管の内面に免疫反応物質を固定化する操作は試験管のそれと比べて著しく容易である。 Operation of immobilizing immunoreactive material on the inner surface of the capillary is significantly easier than that of a test tube. すなわちビーズのときと同様に免疫反応物質の溶液の中に毛細管をまとめて縦方向に浸せば溶液は容易に下部から毛細管の中に充満しこれを一定温度で一定時間放置した後取り出し、洗浄乾燥すればよい。 That is, Hitase longitudinally collectively capillary in a solution of the immune reactants as in the case of bead solution is readily taken out after the filling from the bottom into the capillary for a predetermined time, this in a constant temperature, washed and dried do it. 毛細管の外面にも免疫反応物質は固定化されるが、この管を使用して免疫反応を行なう場合内面しか使用しないので反応には全く支障がない。 Although immunoreactive material to the outer surface of the capillary is immobilized, there is no completely trouble in the reaction since the inner surface only use when performing immunological reaction using this tube.

また、毛細管の場合試験管と比べて直径が小さいので検体液に対する固定化免疫物質の量比が大となるので反応が著しく早くなり試験管法のように回転して反応を促進する必要がない。 The amount ratio of the immobilized immune substance does not need to promote rotation to react as a test tube method the reaction is significantly faster since the large to the analyte solution so as compared with the test tube is smaller in diameter when the capillary . それゆえ毛細管を使用して大量に確実にしかも簡易に自動化操作のできる装置が出現すれば、 If large quantities reliably and appearance device capable of automated operation in a simple to use it because of capillary,
毛細管そのものも小型であるので機械も小型となり、自動化により迅速な大量処理が可能となるはずである。 Machinery becomes small because the capillary itself also small, it should be possible to rapidly mass treatment by automation.

特表昭58−501521に記載された装置は毛細管を用いるものであって、毛細管の内面に種々の化学物質を固定化する方法そのものは既に公知の事実であるので、免疫反応を行なうにあたって免疫反応物質を毛細管の内面に固定化して、これを自動化機械を用いて順次免疫反応を行なうという組み合わせの方法によるものである。 Since the device described in Kohyo Sho 58-501521 be one using a capillary tube, a method for immobilizing a variety of chemical substances on the inner surface of the capillary itself is already known facts immune response carrying out the immunological reaction material was immobilized to the inner surface of the capillary is by a combination of methods that sequentially perform an immune reaction using an automated machine it.

しかしながら上記公報に記載された上述装置によれば毛細管は常に垂直に保たれたまま、種々の操作が行なわれるので毛細管に入れる試薬液が少しでも多くなると毛細管内の表面張力が液の重量をささえきれなくなって、管内から液の一部が流出してしまい正確な化学反応を行なうことができなくなる。 However while the capillary was always vertically maintained according to the above apparatus described in the above publication, the surface tension of the capillary when the reagent solution taken into the capillary tube increases even a little since various operations are performed supporting the weight of the liquid expired longer, some from the tube of the liquid will not be able to perform accurate chemical reaction it will flow out.

毛細管がガラス等の撥水性のない材料で作られている場合、毛細管の下端を水面に接触すると毛管現象によって水が管内に吸い上げられ上昇してくる。 If the capillary tube is made of a material having no water repellency, such as glass, water comes to rise is sucked up into the tube by capillary action upon contact of the lower end of the capillary tube to the water surface. この上昇の高さは管の内径に逆比例して高くなり本発明者らの実験によれば下表のごとくになる。 The height of the increase will be as shown in the table below according to the inverse proportion to higher becomes our experiments to the inner diameter of the tube.

上記のごとく毛細管を垂直にしてその毛細管内に液体を完全に充満して保持できる長さは上表のごとき限界がある。 The length of the liquid can be held completely filled with in its capillary in the vertical of the above as the capillary is limited such table above. それゆえ上記公報に記載された装置において使用できる毛細管はきわめて短い物を使用しなければならない。 Capillary must use very short ones, which can be used in the apparatus described in therefore the above publication.

この装置において垂直に設置された毛細管に対する試料液や試薬液の注入方法は毛細管上部に設けられたカップ中に保持された液体を底部に設けられた小孔を通じて、 Through the small hole injecting method of the sample solution and the reagent solution provided a liquid held in a cup provided in the capillary top to bottom for the installed capillary vertically in the apparatus,
その小孔に毛細管を接触させることにより行なっている。 It is performed by contacting the capillary to the stoma.

この場合も毛細管が長すぎると毛細管の下方のみに液体が保持される。 In this case the liquid only under the the even capillaries are too long capillary is held. また毛細管が短くてもカップに保持された液体の量が毛細管の容積より多い場合、液滴は毛細管下部より落下するか、または液滴として毛細管下部に懸垂する。 Also if the amount of the capillary is held in the cup be shorter liquid is larger than the volume of the capillary, the droplets either dropped from the capillary bottom or suspended capillary lower as droplets. このため測定系に投入する液体の量は不正確になり、これを防ぐためには別に正確な微量ポンプを用意しなければならない。 Therefore the amount of liquid to be introduced into the measurement system becomes inaccurate, must be provided separately accurate trace pump in order to prevent this. それゆえ上記公報において主張するような毛細管を微量ポンプの代用として用いるという簡易性を実現することは困難である。 It is difficult to realize the simplicity of using a capillary as claims Thus in the above publication as a substitute traces pump. また極端に短い毛細管を使用しなければならないので、その毛細管を取り扱う機構は複雑にならざるをえない。 So also must be used extremely short capillary, mechanism to deal with the capillary tube is not forced to become complicated. また、上述の装置によれば、毛細管を垂直にして搬送手段である回転台の周囲に取り付けていたので、その取り付けのために特別の装置が必要となると共に、取り付け及び取り外しの操作がはなはだ面倒なものになっていた。 Further, according to the apparatus described above, because it was attached to the periphery of the turntable is a conveying means of a capillary in the vertical, with the special equipment required for its mounting, operation of mounting and dismounting woefully troublesome It had become a thing. 本発明者らは毛細管を使用する場合の上述の利点を真にいかす装置として全く新しい発想による装置を考案した。 The present inventors have devised a device according to a completely new concept as truly revive device the advantages of the above-described case of using a capillary.

この発明は、従来のそのような欠点を解消するものであって、攪拌装置を必要としないのはもちろん、試薬等の計量装置をも必要とせず、簡単な構造でしかも常に一定量の試薬等が化学反応に供せらて正確な化学反応を行い得るとともに、毛細管の取り付け及び取り外し等の取り扱い操作が容易な化学反応装置を提供することを目的とする。 Accordingly, the present invention is intended to solve the conventional such drawbacks, of course does not require a stirring device is not required even metering device such as reagents, simple structure yet always a fixed amount of a reagent such as There together to obtain make latte exact chemical reactions subjected to a chemical reaction, and it is an object handling operations, such as mounting the capillary tube and removal provides easy chemical reactor.

[問題点を解決するための手段] 上記の問題点を解決するための本発明(第1の発明)による化学反応装置は、複数の毛細管と、これら毛細管を略水平に保持して搬送する搬送手段と、上記毛細管内に供給するための試液等を充填して下端にその試液等の液滴が懸垂されるように上記搬送手段の側部に沿って配置された試液等収容筒体を含む試液等供給手段とを有し、 [Means for solving the problems] The above problems present invention for solving the (first invention) by a chemical reaction apparatus, a plurality of capillaries, the transport holding and conveying these capillaries substantially horizontally comprising means and, the arranged along the side of the conveying means a reagent such as holding cylinder body like droplets of the reagent such as a reagent or the like to the lower end is filled is suspended for supplying into said capillary tube and a reagent solution, such as a supply means,
上記複数の毛細管が上記搬送手段によって搬送される途中で各毛細管の一端が上記試液等収容筒体の下端に懸垂された液滴に触れることによって、毛管現象により試液等が上記各毛細管内に順次供給されるようにしたことを特徴とする。 By end of each capillary in the course of being conveyed touches the liquid droplets suspended in the lower end of the reagent etc. storage cylinder by the plurality of capillaries the conveying means, a reagent such as by capillarity sequentially above in each capillary characterized in that so as to be supplied.

また、本発明に関連する第2の発明は、第1の発明の化学反応装置に、化学反応の結果を測定する測定手段を付加したものである。 The second invention relating to the present invention, the chemical reactor of the first invention, is obtained by adding a measuring means for measuring the result of a chemical reaction.

[作用] 毛細管は、搬送手段によって常にほぼ水平の姿勢を保って搬送され、試液等供給手段の試液等収容筒体の下端に懸垂された液滴に毛細管の一端が触れることによって、 [Operation] capillaries are always conveyed while maintaining a substantially horizontal attitude, by one end of the capillary touches the liquid droplets suspended in the lower end of the reagent etc. storage cylinder of reagent solution, such as a supply means by the conveying means,
毛管現象によって試液等が毛細管内に供給される。 Reagent such as by capillary action is supplied into the capillary. 試液等は毛細管の端部に触れると毛管現象によって瞬時に毛細管内に充填される。 Reagent solution and the like is filled in the capillary instantaneously by capillarity touching the end of the capillary tube. そして毛細管がほぼ水平に保持されているので、毛細管内の液が重力によって管内から落下しようとする力よりも、液を管内に留めようとする表面張力の方が強く、したがって試液等は毛細管の全長にわたって充填された状態で安定状態となり、毛細管内には常に一定量(即ち毛細管の内容積と同量)の試液等が供給、充填される。 And since the capillary is held substantially horizontally, than the force the liquid in the capillary is about to fall from the tube by gravity, stronger towards the surface tension to be Tomeyo liquid into the tube, thus reagent such as capillary becomes a stable state in a state of being filled over the entire length, a reagent and the like always a certain amount in the capillary (i.e. inner volume and the same amount of capillary) is supplied, it is filled.

したがって、化学反応に供せられる試液等の量は、定量ポンプなどを用いることなく常に一定量となり、正確な化学反応を行うことができる。 Accordingly, the amount of reagent or the like that is subjected to chemical reactions, always constant amount without using a metering pump, it is possible to perform accurate chemical reactions.

また毛細管は水平姿勢を保って搬送手段に保持されて搬送されるので、例えば毛細管を搬送手段に載せるだけでも搬送が可能であり、毛細管を搬送手段に保持しあるいは取り外す装置は極めて簡単なもので適応することができる。 Since the capillary is transported held on the transport means while maintaining a horizontal attitude, for example, capillary are possible conveying the just placed on conveying means to hold the capillary tube to transport means or removing apparatus with extremely simple it can be adapted to.

[実施例] 本発明を免疫反応装置に適用した実施例について第1図ないし第3図にもとづいて説明する。 [Example] The present invention based on Figure 1 to Figure 3 for the embodiment applied to the immune reaction apparatus.

第1図は、本実施例装置の一部を省略して示す斜視図である。 Figure 1 is a perspective view showing a partially omitted in this embodiment device.

1は毛細管2を搬送する手段であるベルトコンベアであり、このベルトコンベア1は、減速装置付モータ11により駆動される駆動軸12と、装置本体に回転自在に支持された従動軸13とを両端に備え、それら各軸12, 1 is a belt conveyor is a means for conveying the capillary 2, the belt conveyor 1, both ends a drive shaft 12 driven by a reduction gear with a motor 11, a driven shaft 13 which is rotatably supported on the apparatus main body provided, they each axis 12,
13に各々取着されたプーリ14,15間に例えばポリウレンタン樹脂などの材質よりなる無端の搬送ベルト1 Endless each consisting material such as attached to the example Poriurentan resin between the pulleys 14 and 15 to 13 the conveyor belt 1
6が平行掛けされている。 6 is parallel hook.

上記減速装置付モータ11は、平行掛けされた搬送ベルト16の上側が張り側になるように駆動軸12を回転させるものであり、第1図上では駆動軸12を時計方向に回転させる。 The decelerating device equipped motor 11, which rotates the drive shaft 12 so that the upper tight side of the conveyor belt 16 which is parallel rail, in the first drawing to rotate the drive shaft 12 in a clockwise direction. また、減速装置付モータ11の回転は、ゆるやかな定速回転又はマイクロプロセッサ制御などにより間欠的回転を行うものであってもよく、光センサなどの検知手段によって搬送ベルト16の位置を検出してモータ11の回転を制御するようにしてもよい。 The rotation of the reduction gear with the motor 11, due to gentle constant speed or microprocessor control may be one that performs intermittent rotation, to detect the position of the conveyor belt 16 by the detection means such as an optical sensor it may be to control the rotation of motor 11.

上記搬送ベルト16は、上面が略水平になるように設けられており、その走行方向と直角の向きに、毛細管2を保持する溝17が、搬送ベルト16の外周面に一定間隔で互いに平行になるように形成されている。 The conveyor belt 16, the upper surface is provided so as to be substantially horizontal, in the running direction and perpendicular direction, the grooves 17 for holding the capillary tube 2, in parallel with each other at regular intervals on the outer peripheral surface of the conveyor belt 16 It is formed to be. 溝17の数は使用目的等に合わせて設定できるが、操作性などの点から30〜200 本位が望ましい。 The number of grooves 17 can be set to suit the purpose of use, it is desirable 30-200 oriented from the viewpoint of operability. 尚、搬送ベルト16としてベルトの内周面に凹凸を形成したタイミングベルトを用いて、凹凸付のプーリと咬み合うようにすれば、搬送ベルトとプーリ間のすべりが発生せず、搬送ベルト16 Incidentally, using the timing belt formed with unevenness on the inner circumferential surface of the belt as the conveying belt 16, if so mutually bite the pulley with irregularities, it does not occur slippage between the conveyor belt and the pulley, conveyor belt 16
の走行速度が、より安定したものとなる。 The traveling speed of, becomes more stable. また、溝17 In addition, the groove 17
は毛細管2を安定して保持できる形状であれば、ベルトに多数の櫛歯を立設することにより溝を形成する等どのようなものであってもよい。 If the shape that can hold the capillary tube 2 stably, may be of any type like forming a groove by erecting many comb belt.

上記ベルトコンベア1の従動軸13側端部付近の上方には、毛細管2を搬送ベルト16に供給するホッパ18が設けられている。 Above the vicinity of the driven shaft 13 side end portion of the belt conveyor 1, and supplies the hopper 18 to the conveyor belt 16 the capillary 2 is provided. 第2図はそのホッパ18付近の断面略示図である。 Figure 2 is a schematic cross section of the vicinity of the hopper 18. 毛細管2はガラス製又はポリエチレンその他のプラスチック製であり、例えば内径1〜1.5 mm、全長5〜8cmの大きさで、両端が開口形成されている。 Capillary 2 is made of glass or polyethylene other plastics, for example, an inner diameter of 1 to 1.5 mm, the size of the full-length 5~8Cm, both ends are opened and formed. 毛細管2は、ホッパ18に入れられる前に浸漬その他の方法により内面に抗体など免疫反応物質が付着、固定されており、ホッパ18から重力によって落下して、搬送ベルト16の移動に伴って、ベルト上面の各溝17内に一本ずつ供給されて溝17内に保持され、搬送ベルト16 Capillary 2 is attached immunoreactive substance such as an antibody is to the inner surface by immersion otherwise prior to being placed in the hopper 18 is fixed, to fall from the hopper 18 by gravity, with the movement of the conveyor belt 16, the belt It is supplied one by one on the top surface each groove 17 of is held in the groove 17, the conveyor belt 16
に載せられて搬送される。 It is conveyed placed on.

したがって、搬送手段であるベルトコンベア1に毛細管2を装着するのに特別の装置及び操作等は何ら必要としない。 Accordingly, special equipment and operation, etc. for mounting the capillary tube 2 to the belt conveyor 1 is conveying means does not require any.

また、ベルトコンベア1の駆動軸12側端部付近の下方には処理が終了した毛細管を収納するケース19が設けられており、全ての処理が終了した毛細管は搬送ベルト16の右端部から落下して、上記ケース19内に順次収納される。 Further, below the vicinity of the drive shaft 12 side end portion of the belt conveyor 1 and the case 19 is provided for accommodating the capillary tube processing is completed, the capillaries all processing is completed falls from the right end of the conveyor belt 16 Te, it is sequentially housed in the case 19. したがって、毛細管2をベルトコンベア1から取り外すのにも特別の装置及び操作等は何ら必要としない。 Accordingly, the apparatus and operation of Special also to remove the capillary 2 from the belt conveyor 1 does not require any.

3はベルトコンベア1に沿って設けられ、毛細管2内に被検検体液及び免疫定量薬液などを供給する試薬等供給手段であり、本実施例において試薬等供給手段3は、各毛細管2内に順次異なる被検検体液を供給する被検検体液供給部20、所定時間後に毛細管2内を洗浄して反応の終った被検検体液を排除する被検検体液洗浄部30、 3 is provided along the belt conveyor 1, a reagent, such as a supply means for supplying such test specimen fluid and immunoassay chemical into the capillary tube 2, reagents supply means 3 in this embodiment, in each capillary 2 sequentially different test specimen liquid test sample solution supply unit 20 for supplying, test specimen liquid cleaning unit 30 to eliminate the test specimen liquid end of reaction by washing the capillary 2 after a predetermined time,
次に酵素標識抗体液などの免疫定量試薬液を毛細管2内に供給する酵素標識抗体液供給部40、その所定時間後に毛細管2内を洗浄して結合しなかった酵素標識抗体液を排除する試薬洗浄部50、及び酵素基質液を毛細管2 Then enzyme-labeled antibody liquid immunoassay reagent solution enzymes supplied to the capillary 2 labeled antibody liquid supply part 40, such as, a reagent to eliminate the enzyme-labeled antibody solution that did not bind are washed in the capillary tube 2 after the predetermined time period cleaning unit 50, and enzyme substrate solution capillary 2
に供給する基質液供給部60が順次搬送ベルト16に沿って設けられて構成されている。 Substrate solution supply unit 60 is configured provided sequentially along the transport belt 16 to be supplied to.

被検検体液供給部20は例えば上記ベルトコンベア1と連動して作動する送り装置21に、血液、尿又は唾液など被検検体液が収容された複数の被検検体液収容筒体2 Test sample solution supply unit 20, for example the delivery device 21 that operates in conjunction with the belt conveyor 1, blood, urine or a plurality of such test specimen liquid is accommodated saliva test sample fluid receiving cylinder 2
2が装着されたものである。 2 in which is mounted. その収容筒体22の下端部において被検検体液は水滴を形成して懸垂しており、被検検体液は毛細管2の端部に接触すると毛管現象によって毛細管2内に瞬時に充填される。 And test sample solution was suspended to form a water droplet at the lower end of the housing cylinder 22, the test sample solution is filled immediately into the capillary 2 by capillary action when in contact with an end portion of the capillary tube 2. そして、本発明においては毛細管2が略水平に保持されているので、毛細管2内の液が重力によって管内から落下しようとする力よりも、液を管内に留めようとする表面張力の方が強く、 Then, in the present invention because the capillary 2 is substantially horizontally held, than the force the liquid in the capillary 2 is to fall from the tube by gravity, stronger towards the surface tension to be Tomeyo liquid in the tube ,
被検検体液は毛細管2の全長にわたって充填された状態で安定し、上記収容筒体22の下端に懸垂する被検検体液の量を毛細管2の内容積より大きくすることにより、 By the test sample solution is stable in the state of being filled over the entire length of the capillary tube 2, the amount of the test sample solution for suspending the lower end of the accommodating cylinder 22 larger than the internal volume of the capillary tube 2,
毛細管2内には常に一定量(即ち、毛細管の内容積と同量)の被検検体液が供給、充填されることになる。 Always a certain amount in the capillary 2 (i.e., the same amount as the internal volume of the capillary) test sample solution is supplied, will be filled.

被検検体液洗浄部30については、その断面を略示する第3図を参照しつつ説明すると、搬送ベルト16の一側部には、図示されていない吸引器に接続された吸引管3 For the test specimen liquid cleaning unit 30, will be described with reference to Figure 3 that shows substantially the cross-section, the one side of the conveyor belt 16, the suction pipe 3 connected to a suction device (not shown)
1が、毛細管2に接触しないように接近して設けられている。 1 is provided in proximity so as not to contact the capillary tube 2. このように非接触にすることにより、毛細管に不純物等が付着せず、正確な反応が得られる。 With such a non-contact, impurities do not adhere to the capillary, and the precise reaction is obtained. 搬送ベルト16の他側部には、洗浄液が収容された洗浄液収容筒体32が設けられ、その収容筒体32の下端部において洗浄液が水滴を形成しており、その水滴に毛細管2の端部が接触すると毛管現象によって洗浄液が毛細管2内に充填される。 On the other side of the conveyor belt 16, the cleaning liquid accommodating cylinder 32 which cleaning liquid is accommodated is provided, and the cleaning liquid forms a drop at the lower end of the housing cylinder 32, end of the capillary tube 2 in the water droplets There is filled with the cleaning liquid into the capillary 2 by capillary action upon contact.

上記吸引器は例えばマイクロプロセッサなどにより制御されて、ベルトコンベア1の動きに連動して作動し、毛細管2内に被検検体液収容筒体22を介して被検検体液が充填された後、一定時間経過後、(例えば10分後)にその液を吸引排除し、さらに洗浄液を毛細管2内を通じて一定時間連続的に又は間欠的に吸引して毛細管2内を洗浄する。 Said aspirator is controlled by a microprocessor for example, operates in conjunction with the movement of the belt conveyor 1, after the test sample solution through the test specimen fluid receiving cylinder 22 is filled into the capillary 2, after the elapse of a predetermined time, washing (for example after 10 minutes) the solution was suction-discharge, further predetermined time continuously or intermittently withdrawing to the capillary 2 through the inside of the capillary tube 2 cleaning solution.

酵素標識抗体液供給部40には、上記洗浄液収容筒体3 The enzyme-labeled antibody liquid supply unit 40, the cleaning liquid accommodating cylinder 3
2と同様の酵素標識抗体液収容筒体42が搬送ベルト1 Conveying two similar enzyme-labeled antibody liquid accommodating cylinder 42 Belt 1
6の一側部に設けられ、洗浄された後の毛細管2内に酵素標識抗体液が毛管現象によって充填される。 Provided 6 one side of the enzyme-labeled antibody solution into the capillary 2 after being cleaned is filled by capillary action. そして酵素標識抗体液が毛細管2の全長にわたって充填された状態で安定状態となり、毛細管2内には常に一定量(即ち毛細管の内容積と同量)の酵素標識抗体液が供給、充填される。 The enzyme-labeled antibody solution is a stable state in a state of being filled over the entire length of the capillary tube 2, always enzyme-labeled antibody solution a fixed amount (i.e. internal volume and the same amount of capillary) to the capillary 2 is supplied, is filled.

試薬洗浄部50は上記被検検体液洗浄部30と同じ構造であり、毛細管2内に酵素標識抗体液収容筒体42を介して酵素標識抗体液が充填された後、一定時間経過後に管内の液が吸引排除され毛細管2内が洗浄される。 Reagents cleaning unit 50 has the same structure as the test specimen liquid cleaning unit 30, after the enzyme-labeled antibody solution through the enzyme-labeled antibody liquid storage cylinder 42 is filled into the capillary 2, the pipe after a predetermined time has elapsed liquid is in the suction-discharge capillary 2 is cleaned.

また、基質液供給部60は上記酵素標識抗体液供給部4 Further, the substrate solution supply unit 60 is the enzyme-labeled antibody liquid supply section 4
0と同様の構造により毛細管2内に酵素基質液を充填するものであり、この場合にも毛細管2内には常に一定量(即ち毛細管の内容積と同量)の酵素基質液が供給、充填される。 0 and is intended to fill an enzyme substrate solution into the capillary 2 by a similar structure, the enzyme substrate solution is supplied at all times a certain amount in the capillary 2 in this case (i.e. inner volume and the same amount of capillary), filled It is.

7はベルトコンベアの右端側近傍において、搬送ベルト16を覆うように設けられ、毛細管2内の反応液の光学的濃度を測定する測定手段である。 7 at the right end side near the belt conveyor, is provided so as to cover the conveyor belt 16, a measuring means for measuring the optical density of the reaction liquid in the capillary tube 2. この測定手段は例えば分光光度計や蛍光光度計などが使用可能である。 The measuring means can be used such as a spectrophotometer or fluorometer. そして反応液の光学濃度から被検検体液中の抗原濃度がマイクロコンピュータなどにより演算される。 The antigen concentration of the test sample solution is calculated by a microcomputer or the like from the optical density of the reaction solution. 72はその結果をデジタル表示する表示器である。 72 is a display for digitally displaying the results.

反応液の光学濃度を測定するにあたり、例えば第4図に示されるごとく、出射端部を細く形成した光ファイバ7 Upon measuring the optical density of the reaction solution, for example as shown in Figure 4, the optical fiber was formed thinner the emission end portion 7
1を毛細管2の端部内に挿入して光を出射し、毛細管2 1 the light emitted is inserted into the end of the capillary tube 2, the capillary 2
内を全長に沿って通過した光を検出するようにしてもよい。 Inner may be detecting light passing along the entire length of. このように光ファイバ出射端部を毛細管の端部内に入れて光を出射すると、出射光が全て毛細管の内周壁で反射されながら通過し、毛細管の素材内部を通過するような余分な光が混入しないので正確な測定を行うことができる。 With such an optical fiber emission ends are placed in the end of the capillary tube for emitting light, it passes while the emitted light is reflected by the inner peripheral wall of all capillaries, extra light to pass through the inner capillary material mixed it is possible to perform accurate measurement because it does not. また、第11図に示されるごとく、検出光が、 Further, as shown in FIG. 11, the detection light,
搬送ベルト16上に水平に保持された毛細管2を垂直に通過するようにしてもよい。 It may be passed through the capillary tube 2 which is horizontally held on the conveyor belt 16 vertically.

次に、CEA(カルチノ エンブリオニック アンチゲン)の酵素免疫測定法により、被検検体液中のCEA濃度を測定する場合を例にとって、上記実施例の動作について説明する。 Then, by an enzyme immunoassay CEA (Karuchino Embryo Nick Antigene), taking the case of measuring the concentration of CEA in the test sample solution, the operation of the above embodiment.

毛細管に付着させておく免疫反応物質として抗CEA抗体、酵素標識抗体として酵素標識抗CEA抗体、基質として過酸化水素、発色剤としてオルトフェニレンジアミン、標識酵素としてはパーオキシダーゼを用いた。 Anti-CEA antibody as immunoreactive substance to be adhered to the capillary, the enzyme-labeled anti-CEA antibody as an enzyme-labeled antibody, hydrogen peroxide as a substrate, o-phenylenediamine as a chromogenic agent, a labeled enzyme using peroxidase.

毛細管2は内面に抗CEA抗体を付着させてホッパ18 Capillary 2 by attaching an anti-CEA antibody to the inner surface of the hopper 18
内に多数入れておく。 It puts a number on the inside. また、各被検検体液収容筒体22 Each test specimen liquid accommodating cylinder 22
に各々被検検体液を入れて、被検検体液供給部20の送り装置21に装着しておく。 Each put a test sample fluid, keep attached to the delivery device 21 of the test sample solution supply unit 20 to the.

ベルトコンベア1を作動させると、搬送ベルト16の移動に伴ってホッパ18から毛細管2が落下して、毛細管2はベルトの溝17内に一本ずつ略水平に、かつ互いに平行に保持され、搬送ベルト16に載せられて搬送される。 Operating the conveyor belt 1, to fall capillary 2 from the hopper 18 with the movement of the conveyor belt 16, the capillary 2 is substantially horizontally one by one into the groove 17 of the belt, and are held parallel to one another, the transport It is transported placed on the belt 16.

そして、被検検体液供給部20で毛細管2内に被検検体液が充填されて免疫反応が行われ、液中に含まれるCE Then, the test sample fluid is filled into the capillary 2 immune response is performed in the test sample solution supply unit 20, contained in the liquid CE
Aが毛細管2内に付着された抗CEA抗体に結合される。 A is bound to the anti-CEA antibody attached to the capillary 2.

搬送ベルト16はゆっくりと移動し、その間毛細管2内では免疫反応が進み、被検検体液充填から所定時間経過後(例えば10分後)に、被検検体液洗浄部30において毛細管2内が洗浄され、抗CEA抗体と結合したCEA Conveyor belt 16 moves slowly, during the immune reaction proceeds within the capillary tube 2, after the lapse of a predetermined time from a test sample liquid filling (for example, after 10 minutes), is the capillary 2 in the subject test solution cleaning part 30 wash is, CEA bound to anti-CEA antibody
以外の液が全て排出される。 Liquid other than is discharged all.

次いで、酵素標識抗体液供給部40において、毛細管2 Then, the enzyme-labeled antibody liquid supply unit 40, the capillary 2
内に酵素標識抗CEA抗体が充填され、管内に固定されているCEAを介していわゆるサンドイッチ式に酵素標識CEA抗体が管内に固定される。 Enzyme-labeled anti-CEA antibody is filled, the enzyme-labeled CEA antibody in a so-called sandwich is fixed to the tube via the CEA which is fixed to the tube within. そしてその所定時間経過後(例えば10分後)に、酵素標識抗体液洗浄部50 And the after a predetermined time (e.g. after 10 minutes), enzyme-labeled antibody liquid cleaning unit 50
において毛細管2内が洗浄され、管内に固定されなかった酵素標識抗体液が全て排出される。 The capillary 2 is washed, enzyme-labeled antibody solution which has not been fixed in the tube is discharged all at.

続いて酵素基質液供給部60において、過酸化水素水とオルトフェニレンジアミンの混合液が毛細管2内に充填され、管内に固定された酵素標識抗体の酵素(パーオキシダーゼ)によって過酸化水素から分解された酸素がオルトフェニレンジアミンと反応して液が発色する。 In an enzyme substrate solution supplying section 60 is followed, a mixture of hydrogen peroxide and o-phenylenediamine is filled into the capillary 2 is decomposed hydrogen peroxide by the enzyme (peroxidase) of the enzyme-labeled antibody immobilized in the tube oxygen the liquid is colored by reaction with o-phenylenediamine.

以上の反応工程中において、毛細管2は常にほぼ水平の姿勢を保っているので搬送ベルトの溝17内に安定して保持されており、また、管内に各種の試薬等が充填されたときはその試薬等が毛管現象によって管内全長にわたって充填保持され、しかも管内からこぼれない。 During more reaction steps, the capillary 2 is always held stably substantially in the horizontal groove of the conveyor belt because it keeps the posture 17, also when the various reagents or the like is filled in the tube is the reagents and the like is filled and held over the pipe full length by capillarity, yet does not spill from the tube. したがって反応に用いられる試薬等の量は常に毛細管の内容積と同量となり、計量装置類を全く設けることなく正確な反応をさせることができる。 Thus the amount of reagents used in the reaction is always capillary having an inner volume and the same amount, it can be an accurate reaction without providing a metering device such at all.

尚、管内液が発色した毛細管2は、所定時間経過後(例えば10分後)に測定手段7に到達し、反応の結果が比色計などの読みによって読み取られ、その値がマイクロコンピュータ等で演算されて例えば表示器72にデジタル表示される。 Incidentally, the capillary 2 which pipe liquid is colored reaches the measuring means 7 after a predetermined time has elapsed (e.g. after 10 minutes), the result of the reaction is read by the reading such as colorimeter, the value is a microcomputer or the like is digitally displayed on is computed for example, the display 72.

そして、全ての処理が終了した毛細管2は搬送ベルト1 The capillary tube 2 all processing is completed conveyor belt 1
6の右端部から落下してケース19内に順次収納される。 Falls from the right end of the 6 are sequentially housed in the case 19.

さらにAFP(α−フェトプロティン)の酵素免疫測定法により、被検検体液中AFP濃度を測定する場合を例にとって、上記実施例の動作について説明する。 Furthermore by an enzyme immunoassay AFP (alpha-fetoprotein), taking the case of measuring the test specimen fluid AFP concentration, the operation of the above embodiment.

毛細管に付着させておく免疫反応物質として抗AFP抗体、酵素標識抗体として酵素標識抗AFD抗体、酵素基質として過酸化水素、発色剤としてオルトフェニレンジアミン、標識酵素としてはパーオキシダーゼを用いた。 Anti-AFP antibody as immunoreactive substance to be adhered to the capillary, the enzyme-labeled anti AFD antibody as an enzyme-labeled antibody, hydrogen peroxide as an enzyme substrate, o-phenylenediamine as a chromogenic agent, a labeled enzyme using peroxidase.

毛細管2は内面に抗AFP抗体を付着させて、さらに酵素標識抗体を内面にコーティングして凍結乾燥し、ホッパ18内に多数入れておく。 Capillary 2 by attaching an anti-AFP antibody to the inner surface, further coating and lyophilized enzyme-labeled antibody on the inner surface, it puts a number in the hopper 18. また、各被検検体液収容筒体22に各々被検検体液を入れて、被検検体液供給部2 In addition, each placed a test sample fluid to each test specimen liquid accommodating cylinder 22, the test sample solution supply section 2
0の送り装置21に装着しておく。 Keep attached to 0 the feeder 21.

ベルトコンベア1を作動させると、搬送ベルト16の移動に伴ってホッパ18から毛細管2が落下して、毛細管2はベルトの溝17内に一本ずつ略水平に、かつ互いに平行に保持され、搬送ベルト16に載せられて搬送される。 Operating the conveyor belt 1, to fall capillary 2 from the hopper 18 with the movement of the conveyor belt 16, the capillary 2 is substantially horizontally one by one into the groove 17 of the belt, and are held parallel to one another, the transport It is transported placed on the belt 16.

そして、被検検体液供給部20で毛細管2内に被検検体液が充填されて免疫反応が行なわれる。 The immune reaction is carried out is the test specimen liquid filled in the capillary tube 2 in the test sample solution supply unit 20.

搬送ベルト16はゆっくりと移動し、その間毛細管2内では内面に付着させた抗AFP抗体と被検検体液中に存在するAFPとの反応、さらには内面に付着させた抗A Conveyor belt 16 moves slowly, anti-A which during reaction with the AFP in the capillary tube 2 present anti-AFP antibody and the test sample solution that was attached to the inner surface, and further adhered to the inner surface
FD抗体と反応したAFPと酵素標識抗AFP抗体との免疫反応が進み、被検検体液充填から所定時間経過後(例えば15分後)に、試薬洗浄部50において毛細管2内が洗浄され内面に付着した抗AFP抗体と結合したAFPおよびこのAFPを介して結合した酵素標識抗A Immune reaction between AFP and the enzyme-labeled anti-AFP antibody that reacted with FD antibody proceeds, after a predetermined time has elapsed from the test specimen liquid filling (for example, after 15 minutes), the inner surface being cleaned the capillary 2 in the reagent cleaning unit 50 enzyme-labeled anti-a bound via the bound AFP and the AFP and anti-AFP antibody attached
FP抗体以外の反応にあずからなかった液が全て排出される。 Liquid did Azukara to reactions other than FP antibody is discharged all.

ここまでの反応が一度に行なわれるので被検検体洗浄部30および酵素標識抗体液供給部40は停止したままとなる。 Since the reaction up to here is performed at one time test subject cleaning unit 30 and the enzyme-labeled antibody liquid supply unit 40 remains stopped.

続いて酵素基質液供給部60において、過酸化水素水とオルトフェニレンジアミンの混合液が毛細管2内に充填され、管内に固定された酵素標識抗体の酵素(パーオキシダーゼ)によって過酸化水素から分解された酵素がオルトフェニレンジアミンと反応して液が発色する。 In an enzyme substrate solution supplying section 60 is followed, a mixture of hydrogen peroxide and o-phenylenediamine is filled into the capillary 2 is decomposed hydrogen peroxide by the enzyme (peroxidase) of the enzyme-labeled antibody immobilized in the tube enzyme the liquid is colored by reaction with o-phenylenediamine.

次に管内液が発色した毛細管2は、所定時間経過後(例えば10分後)に測定手段7に到達し、反応の結果が比色計などによって読み取られ、その値がマイクロコンピュータ等で演算されて例えば表示器72にデジタル表示される。 Capillary 2 Then the tube liquid is colored reaches the measuring means 7 after a predetermined time has elapsed (e.g. after 10 minutes), the result of the reaction is read by such colorimeter, the value is calculated by a microcomputer or the like digitally displayed Te for example, the display 72.

そして全ての処理が終了した毛細管2は搬送ベルト16 The capillary tube 2 which all processing is finished conveyor belt 16
の右端部から落下してケース19内に順次収納される。 Drops from the right end portion are sequentially stored in the case 19.

第5図及び第6図は搬送手段であるベルトコンベアの形状を変えた例を示しており、反応液の光学的濃度を測定手段7において測定する際に、測定用光線を装置の垂直方向(Y方向)に発射する場合などには、第5図に示されるように搬送ベルト116の溝117の底部に測定用光線が通過するための孔118を穿設し、あるいは第6 Figure 5 and Figure 6 shows an example of changing the shape of the belt conveyor is a conveying means, when measuring the optical density of the reaction liquid in the measuring means 7, the vertical direction of the measuring beam device ( the example, when firing in the Y direction), drilled holes 118 for measuring light in the bottom of the groove 117 of the conveyor belt 116 as shown in FIG. 5 passes, or 6
図に示されるように、間隔をあけて平行に設けられた一対の搬送ベルト216に毛細管2を載せて、そのベルト間を測定用光線が通過するようにしてもよい。 As shown, put the capillary tube 2 to the pair of conveying belts 216 provided in parallel at intervals, may be between the belt measuring beam passes.

尚、上記実施例においては毛細管を一本ずつ独立して供給したが、第7図に示されるように複数の毛細管2を平行に並べて、例えば紙又はプラスチック製のシート4等に接着その他の手段で装着、連結してもよい。 In the above embodiment was supplied independently one by one capillary, but arranged in parallel a plurality of capillary 2 as shown in FIG. 7, for example, adhesive or other means to the paper or plastic sheet 4 or the like in mounting, it may be connected. このようにすると、各種の表示や記録をシート4の裏面に記載することができて実用上も便利である。 In this way, practically also convenient to be able to describe the various display or recording on the back surface of the sheet 4. 5は測定用光線が通過するように穿設された孔である。 5 is a hole in which the measuring beam is bored to pass.

また、上記実施例においては搬送手段としてベルトコンベアを用いたが、本発明はこれに限定されるものではなく、第8図に示されるように、例えば搬送手段を回転するドーナツ状の円盤301で形成し、その上面に溝31 Further, in the above embodiment using a belt conveyer as the conveying means, the present invention is not limited to this, as shown in FIG. 8, for example, a donut-shaped disc 301 to rotate the conveying means formed, grooves 31 on its upper surface
7を放射状に形成してそこに毛細管を載せてもよい。 7 may rest capillaries therein formed radially. この場合にも、毛細管2は等間隔(即ち等角度)で搬送手段に保持されるのが、各種制御にとって都合がよく、第7図の場合と同様に、予め複数本の毛細管をシートに放射状に装着しておいてもよい。 Radially in this case, that the capillary tube 2 is held in the conveying unit at regular intervals (i.e., equal angles), is convenient for various control, as in the case of Figure 7, the pre plurality of capillaries in a sheet it may have been attached to. また、本発明の化学反応装置は、例えば第9図及び第10図に示されるように、 The chemical reaction apparatus of the present invention, as shown for example in Figure 9 and Figure 10,
周部に溝417が形成されて水平軸中心に回転する円形の回転体401の溝417に、毛細管2を保持して搬送する等、他の種々の形式による搬送装置を用いてもよい。 The grooves 417 of the circular rotating member 401 which rotates a horizontal shaft center groove 417 is formed in the peripheral portion, etc. which holds and conveys a capillary tube 2, may be used conveying device according to still another different form.

また、上記実施例は本発明を免疫反応装置に適用したものを示したが、本発明は[産業上の利用分野]に記載したごとく、他の種々の化学反応装置に適用されるものを含むものである。 Further, the above embodiment is the present invention showed that applied in the immune response system, the present invention is as described in [BACKGROUND OF THE, those applied to other various chemical reactor including is Dressings.

[発明の効果] 本発明の化学反応装置によれば、毛細管を水平に保持して搬送する途中で、各毛細管の一端が試液等収容筒体の下端に懸垂された試液等の液滴に触れることによって、 According [Effect of the Invention] the chemical reactor of the present invention, in the course of conveying the capillary tubes held horizontally, touch the droplets of reagent or the like which is suspended at the lower end of one end reagent etc. storage cylinder of each capillary by,
毛管現象により試液等が各毛細管内に順次供給されるようにしたので、搬送手段によって毛細管を搬送する運動を行うだけの極めてシンプルな構造で各毛細管内に試液等を供給することができ、しかも、毛細管内に供給、充填されて反応に供せられる試液等の量が常に一定となり、計量装置類を全く用いることなく極めて正確な化学反応を行うことができる。 Since reagent such as by capillarity has to be sequentially supplied into each capillary, it can supply the reagent such as in each capillary in a very simple structure of only performing motion to convey the capillary by the conveying means, moreover , supplied to the capillary, the amount of such reagent to be subjected to the reaction is filled with always constant, it is possible to perform very accurate chemical reactions without the use of a metering device such at all. したがって、装置が簡素化されて製造コストも低価となり、また誰にでも使い易くメインテナンスなども容易なものとなり、さらに、毛細管を搬送手段に保持しあるいは取り外す装置が極めて簡単なもので済むので、その操作も容易で使い易いものが得られる等の効果を有し、自動的に化学反応を行うのに適しかつ簡便で使い易い化学反応装置を得ることができる。 Thus, the device also becomes low cost manufacturing cost is simplified, also anyone liable maintenance etc. becomes as easy to use even in further, since removal held or transport means a capillary device requires only extremely simple, has the effect of such the operation as easy and easy to use is obtained, it is possible to obtain easy-to-use chemical reactor automatically and conveniently suitable for performing chemical reactions.

【図面の簡単な説明】 BRIEF DESCRIPTION OF THE DRAWINGS

第1図は本発明の一実施例装置の一部を省略して示す斜視図、第2図はその装置のホッパ付近の断面略示図、第3図はその被検検体液洗浄部の断面図、第4図は測定手段に用いられる光出射部の一例の略示図、第5図及び第6図はベルトコンベアの異なる形状の例を示す部分斜視図、第7図は複数の毛細管を連結した状態を示す斜視図、第8図は搬送手段の他の実施例を示す部分斜視図、 Figure 1 is a perspective view showing a partially omitted of an embodiment apparatus of the present invention, Figure 2 is schematic cross section of the vicinity of the hopper of the apparatus, FIG. 3 is cross section of the test specimen liquid cleaning unit Figure, an example of a schematic view of the light emitting portion used in Fig. 4 measuring means, Figure 5 and Figure 6 is a partial perspective view showing an example of a different conveyor belt shape, FIG. 7 is a plurality of capillaries perspective view showing the connecting state, partial perspective view FIG. 8 is showing another embodiment of the conveying means,
第9図は搬送手段のさらに異なる実施例の正面図、第1 Figure 9 is a front view of still another embodiment of the conveying means, first
0図はその平面図、第11図は測定手段に用いられる光出射部の他の一例の略示図であり(a)は正面断面図、 0 Figure is a plan view, FIG. 11 is a schematic view of another example of a light emitting portion for use in measuring means (a) is a front sectional view,
(b)はその側面断面図である。 (B) is a side sectional view. 1…ベルトコンベア(搬送手段)、2…毛細管、3…試薬等供給手段、7…測定手段、16…搬送ベルト、17 1 ... belt conveyor (conveying means), 2 ... capillary, 3 ... reagents supply means, 7 ... measuring means, 16 ... conveyor belt, 17
…溝、20…被検検体液供給部、30…被検検体液洗浄部、40…酵素抗体液供給部、50…試薬洗浄部、60 ... groove, 20 ... test specimen liquid supply unit, 30 ... test specimen liquid cleaning unit, 40 ... enzyme antibody solution supply unit, 50 ... reagent cleaning unit, 60
…基質液供給部、116…搬送ベルト、117…溝、2 ... substrate solution supplying unit, 116 ... conveyor belt, 117 ... groove, 2
16…搬送ベルト、301…円盤、317…溝、401 16 ... conveying belt, 301 ... disc, 317 ... groove, 401
…回転体、417…溝。 ... rotating body, 417 ... groove.

Claims (17)

    【特許請求の範囲】 [The claims]
  1. 【請求項1】複数の毛細管と、これら毛細管を略水平に保持して搬送する搬送手段と、上記毛細管内に供給するための試液等を充填して下端にその試液等の液滴が懸垂されるように上記搬送手段の側部に沿って配置された試液等収容筒体を含む試液等供給手段とを有し、上記複数の毛細管が上記搬送手段によって搬送される途中で各毛細管の一端が上記試液等収容筒体の下端に懸垂された液滴に触れることによって、毛管現象により試液等が上記各毛細管内に順次供給されるようにしたことを特徴とする化学反応装置。 And 1. A plurality of capillaries, and conveying means for holding and conveying these capillaries substantially horizontally, the droplets of the reagent such as the lower end is filled with a reagent and the like to be supplied to the said capillary tube is suspended so that the and a reagent solution, such as a supply means including a side disposed along the reagent etc. storage cylindrical body of the conveying means, the middle one end of each capillary that said plurality of capillaries is conveyed by the conveying means by touching the liquid droplets suspended in the lower end of the reagent etc. storage cylinder, a chemical reactor reagent such as by capillarity is characterized in that so as to be sequentially supplied to the in each capillary.
  2. 【請求項2】上記複数の毛細管が互いに平行に上記搬送手段に保持されている特許請求の範囲第1項記載の化学反応装置。 2. A chemical reactor of Claim 1 wherein the appended claims the plurality of capillaries is held in parallel to the conveying means with each other.
  3. 【請求項3】上記複数の毛細管が放射状に上記搬送手段に保持されている特許請求の範囲第1項記載の化学反応装置。 Wherein said plurality of capillaries chemical reactor of Claim 1 wherein the claims held in the conveying means radially.
  4. 【請求項4】上記複数の毛細管が一定間隔で上記搬送手段に保持されている特許請求の範囲第2項又は第3項記載の化学反応装置。 Wherein said plurality of ranges of the capillary tube according patents held in said conveying means at regular intervals the second term or third term chemical reactor according.
  5. 【請求項5】上記毛細管が、予め複数本連結されている特許請求の範囲第4項記載の化学反応装置。 Wherein said capillary tube, previously plurality of concatenated in that patent chemical reactor ranging fourth claim of claim.
  6. 【請求項6】上記搬送手段がベルトコンベアであり、そのベルトに毛細管を保持する溝が形成されている特許請求の範囲第1項ないし第5項のいずれかの項に記載の化学反応装置。 Wherein a said conveyor means belt conveyor, a chemical reactor according to any one of claims of claim 1, wherein patent groove for holding is formed capillaries in the belt to paragraph 5.
  7. 【請求項7】上記搬送手段が、周部に溝が形成された円形の回転体であり、その溝に毛細管が保持されて搬送される特許請求の範囲第1項ないし第4項のいずれかの項に記載の化学反応装置。 7. The conveying means is a circular rotary member with grooves formed in the periphery, either in its first term the claims capillary is conveyed while being held in the groove to paragraph 4 the chemical reaction device described in the section.
  8. 【請求項8】上記試液等収容筒体の下端に懸垂される液滴の量が、上記毛細管の内容積より大きい特許請求の範囲第1項ないし第7項のいずれかの項に記載の化学反応装置。 8. The amount of droplets to be suspended at the lower end of the reagent etc. storage cylinder is chemical according to any one of the paragraphs above paragraph 1 range of internal volume greater than the claims of the capillary to paragraph 7 reactor.
  9. 【請求項9】上記試液等供給手段が、毛細管内に供給された試液等を排出して毛細管内を洗浄する洗浄手段を含んでいる特許請求の範囲第1項ないし第8項のいずれかの項に記載の化学反応装置。 9. The reagent solution, such as a supply means, the range paragraphs 1 through 8 wherein claims containing a cleaning means for discharging the supplied into the capillary test solution or the like to clean the inside capillary either chemical reactor according to claim.
  10. 【請求項10】上記洗浄手段が、毛細管に接触することなく毛細管内の試液等を吸引排出する吸引手段を含んでいる特許請求の範囲第9項記載の化学反応装置。 10. The cleaning means, the chemical reaction apparatus of Claims paragraph 9, wherein the reagent solution in the capillary tube or the like includes a suction means for sucking discharge without contacting the capillary.
  11. 【請求項11】上記洗浄手段における吸引手段が設置された毛細管の反対側に、洗浄液供給手段を含んでいる特許請求の範囲第9項または第10項記載の化学反応装置。 11. The opposite side of the capillary suction means is installed in the cleaning unit, a chemical reactor ranges paragraph 9 claims that contains a cleaning liquid supply means or paragraph 10, wherein.
  12. 【請求項12】上記毛細管の内面に予め免疫反応物質が付着され、上記試液等供給手段が検体と試液とを所定の時間間隔で毛細管内に供給する特許請求の範囲第1項ないし第11項のいずれかの項に記載の化学反応装置。 12. advance immunoreactive material to the inner surface of the capillary tube is attached, the first term claims to be supplied to the capillary and reagent solution sample and the reagent, such as a supply means at predetermined time intervals to paragraph 11 the chemical reaction device according to any one of the paragraphs.
  13. 【請求項13】複数の毛細管と、これら毛細管を略水平に保持して搬送する搬送手段と、上記毛細管内に供給するための試液等を充填して下端にその試液等の液滴が懸垂されるように上記搬送手段の側部に沿って配置された試液等収容筒体を含む試液等供給手段とを設けて、上記複数の毛細管が上記搬送手段によって搬送される途中で各毛細管の一端が上記試液等収容筒体の下端に懸垂された液滴に触れることによって、毛管現象により試液等が上記各毛細管内に順次供給されるようにすると共に、さらに毛細管中での化学反応の結果を測定する測定手段を設けたことを特徴とする化学反応装置。 13. A plurality of capillaries, and conveying means for holding and conveying these capillaries substantially horizontally, the droplets of the reagent such as the lower end is filled with a reagent and the like to be supplied to the said capillary tube is suspended provided a reagent solution, such as a supply means comprising said arranged along the side of the conveying means a reagent such as holding cylinder body so that, in the middle at one end of each capillary that said plurality of capillaries is conveyed by the conveying means by touching the liquid droplets suspended in the lower end of the reagent etc. storage cylinder, with a reagent or the like by the capillary phenomenon to be sequentially supplied to the in each capillary, further measure the results of chemical reactions in a capillary chemical reactor, wherein a provided measurement means for.
  14. 【請求項14】上記測定手段が、毛細管内の反応液の光学的濃度を測定する光学的濃度測定手段を含んでいる特許請求の範囲第13項に記載の化学反応装置。 14. the measuring means, a chemical reactor according to Section 13 claims comprising an optical density measuring means for measuring the optical density of the reaction solution in the capillary tube.
  15. 【請求項15】上記光学的濃度測定手段が、出射端部が細く形成されて毛細管の端部内に挿入された光学ファイバを有し、その光ファイバから出射されて毛細管内を全長に沿って通過又は散乱した光を検出する特許請求の範囲第14項記載の化学反応装置。 15. The optical density measuring means is formed exit end thinner have inserted optical fiber into the end of the capillary, along the entire length of the emitted by capillary from the light passing through the fiber or scattered chemical reactor ranges paragraph 14, wherein claims for detecting light.
  16. 【請求項16】上記光学的濃度測定手段が、測定用の光線が毛細管に垂直方向に横断した透過光又はその際散乱した光を検出する特許請求の範囲第14項記載の化学反応装置。 16. The optical density measuring means, light chemical reactor ranges paragraph 14, wherein claims for detecting the light scattered when the transmitted light or the traversed vertically capillary for measurement.
  17. 【請求項17】上記毛細管の内面に予め免疫反応物質が付着され、上記試液等供給手段が検体と試液とを所定の時間間隔で毛細管内に供給する特許請求の範囲第13項ないし第16項のいずれかの項に記載の化学反応装置。 17. advance immunoreactive material to the inner surface of the capillary tube is attached, the scope Section 13 of the claims to be supplied to the capillary and the reagent solution, such as a supply means sample and reagent solution at a predetermined time interval to paragraph 16 the chemical reaction device according to any one of the paragraphs.
JP21452186A 1986-09-11 1986-09-11 The chemical reaction device Expired - Lifetime JPH067918B2 (en)

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JP21452186A JPH067918B2 (en) 1986-09-11 1986-09-11 The chemical reaction device
CA 544781 CA1289856C (en) 1986-09-11 1987-08-18 Chemical reaction apparatus
US07087646 US4960566A (en) 1986-09-11 1987-08-19 Chemical reaction apparatus
AU7737287A AU582501B2 (en) 1986-09-11 1987-08-21 A chemical reaction apparatus
AT87308002T AT69892T (en) 1986-09-11 1987-09-10 Apparatus for chemical reactions.
ES87308002T ES2028095T3 (en) 1986-09-11 1987-09-10 Chemical reaction apparatus.
EP19870308002 EP0260136B1 (en) 1986-09-11 1987-09-10 Chemical reaction apparatus
DE19873774810 DE3774810D1 (en) 1986-09-11 1987-09-10 Apparatus for chemical reactions.

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JP5477341B2 (en) * 2011-06-01 2014-04-23 株式会社島津製作所 Microchip electrophoresis method and apparatus
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JP3915652B2 (en) 2002-10-09 2007-05-16 コニカミノルタビジネステクノロジーズ株式会社 Image processing apparatus

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US3915652A (en) * 1973-08-16 1975-10-28 Samuel Natelson Means for transferring a liquid in a capillary open at both ends to an analyzing system

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