JPH0678749A - Microbe detecting instrument - Google Patents

Abstract

PURPOSE:To provide a microbe detecting instrument highly easy in microbe detecting operations without the need for using many implements. CONSTITUTION:The instrument has (A) the vessel body 3 provided with a breakable part 11 and (B) a microbe trapping member 8 held in the vessel body 3. This body 3 is divisible into a vessel 3a adjacent to said member 8 and a cap 3b through the breakable part 11; the cap 3b, in turn, has (1) a housing part 3e for the edge of said member side and (2) a medium 5 for detecting microbes put into or stuck inside the housing part 3e. By pressing the vessel 3a, a microbe trapping liquid 6 filled inside the member 8 can be discharged; and the medium 5 contains a detecting reagent which changes in its color tone due to the proliferation of the target microbes.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a novel microorganism detecting instrument which can easily detect the presence of microorganisms.

[0002]

2. Description of the Related Art Conventionally, various instruments and culture media have been used for detecting microorganisms. However, in the conventional microorganism detection instrument, first, from the detection site, for example,
It is necessary to collect samples (bacteria, microorganisms) by the wiping method, stamp method, etc., transfer the collected samples (bacteria, microorganisms) to a detection medium suitable for the bacteria to be detected, and culture for detection. . Therefore, a device for collecting samples (bacteria, microorganisms) from the detection site, a device for transplanting the collected samples (bacteria, microorganisms) into the culture medium, a device for storing the culture medium, and capable of culturing are the minimum required. Met.

[0003]

As described above, in the conventional method, many instruments must be used for detecting microorganisms, and the collected sample (bacteria, microorganisms) is used as a medium. Have to do the work of porting to
The work was complicated. MRSA (methicillin-resistant Staphylococcus aureus, Staphylococcus aureus resistant to many antibiotics), which is a staphylococcus resistant to many antibiotics, is one of the causes of nosocomial infections that have recently become a social problem. Infection. This MRSA is for patients, doctors,
It is present on nurses' fingers, nasal passages, clothes, walls of hospital rooms, beds, etc. and causes serious symptoms in immunocompromised persons. Therefore, some facilities have created their own nosocomial infection control manuals to manage the environment. However, implementation of environmental management requires a lot of effort and cost, so many facilities cannot implement it while feeling the necessity of management. In particular, MRSA
In order to check the presence of the sample, as described above, a sample (bacteria, microorganisms) is collected from the detection site, and the collected sample (bacteria,
It is necessary to transplant the microorganism) into a detection medium suitable for the sample (bacteria, microorganisms) to be detected and to culture it. For this reason, the equipment for collecting samples (bacteria, microorganisms) from the detection site, the equipment for transplanting the collected samples (bacteria, microorganisms) into the culture medium, and the equipment for storing and culturing the culture medium are the minimum. There is a problem in that it is necessary, various instruments must be prepared, and the work is not easy.

Therefore, an object of the present invention is to solve the above-mentioned problems and to perform detection work extremely easily without using many instruments for detecting microorganisms as in the conventional case. The present invention provides a device for detecting microorganisms. Further, an object of the present invention is to solve the above-mentioned problems and to detect MRSA as in the prior art, without using many instruments, and furthermore, the detection work can be performed extremely easily. The equipment is provided.

[0005]

[Means for Solving the Problems] [Means for Solving the Problems] [Means for Solving the Problems] A container body having a rupturable portion and a microorganism trapping member housed in the container body are provided, and the container body is rupturable. By the part, it is possible to divide into a microorganism trapping member side container and a cap portion, and further, the cap portion, after the division, a storing portion for storing the microorganism trapping member side end portion of the microorganism trapping member side container, The microorganism detecting device has a culture medium stored or attached inside the storage portion. Then, it is preferable that the inside of the container for trapping microorganisms is filled with a liquid for trapping microorganisms. Also,
The microorganism trapping member preferably has a liquid absorbing property. Furthermore, it is preferable that the microorganism-capturing member-side container is capable of flowing out the microorganism-trapping liquid filled in from the microorganism-trapping member by pressing. Furthermore, it is preferable that the medium contains a detection reagent whose color tone changes due to the growth of the target microorganism. Further, the medium is preferably a dry medium. Further, it is preferable that the cap portion has an opening portion that fits into a divided end portion of the microorganism-capturing member-side container after division and a storage portion for the exposed portion of the microorganism-capture member. Furthermore, it is preferable that the cap portion is transparent to the extent that the color tone of the inside can be confirmed from the outside.

Further, what achieves the above object has a container main body having a breakable portion and a microorganism capturing member housed in the container main body, wherein the container main body is provided with the microorganisms by the breakable portion. The capturing member side container and the cap portion are separable, and further, the cap portion is, after the division, a storing portion for storing a microorganism capturing member side end portion of the microorganism capturing member side container, and inside the storing portion. Having a microorganism detection medium housed or attached, further, the medium is a methicillin-resistant Staphylococcus aureus detection medium, and
A microorganism detection instrument containing a detection reagent for detecting the presence of methicillin-resistant Staphylococcus aureus by discoloration.

Further, what achieves the above-mentioned object is a container body which is divided into a microorganism-capturing member side container and a cap part by breaking or opening the breakable or openable part or separating the fitted part, and the container. The main body comprises a microorganism-trapping member housed in the microorganism-trapping member-side container, and the microorganism-trapping member-side container is formed by breaking or opening the breakable or opening-capable portion, or separating the fitted portion. And a microbial trapping member pressing portion for projecting the tip of the microbial trapping member from the opening and storing it again in the microbial trap member side container, and further, the cap portion The microorganism detecting device is characterized in that it has a storage portion for storing the opening of the microorganism capturing member side container, and a medium stored or attached inside the storage portion. . Further, it is preferable that the pressing portion is an expansion / contraction portion that expands / contracts by pressing. Furthermore, it is preferable that the expandable portion is formed of a bellows dropper. Moreover, it is preferable that the pressing portion is a deformable portion that is deformed into the microorganism-capturing member-side container by pressing. Furthermore, the pressing portion is to restore the original shape by releasing the pressing of the pressing portion, and automatically stores the microorganism capturing member in the microorganism capturing member side container. preferable. The inside of the container body is preferably filled with a liquid for capturing microorganisms. Further, it is preferable that the rear end of the microorganism capturing member is fixed to the inner surface of the rear end of the container body. Further, it is preferable that the microorganism trapping member has a liquid absorbing property. It is preferable that the microorganism-trapping liquid can flow out to the outside by the expansion and contraction of the expansion and contraction section or by pressing the side surface of the container body. Further, it is preferable that the medium contains a detection reagent whose color tone is changed by the propagation of the target microorganism. Further, the medium is preferably a dry medium. Further, it is preferable that the cap portion has a rear end opening that fits with a front end portion of the container body. Further, it is preferable that the cap portion has a tip end opening that fits with a tip end portion of the container body. Further, the cap portion is
It is preferable that a medium storage part for storing the medium is formed and an inner cylinder part that can be inserted into the opening of the container body is provided. Furthermore, it is preferable that at least a part of the container body or the cap portion has transparency to the extent that the color tone of the inside can be confirmed from the outside. Further, it is preferable that the cap portion has a closing portion that makes the front end opening and the rear end opening unable to communicate with each other.

Further, what achieves the above-mentioned object is a container body divided into a microorganism-capturing member side container and a cap part by breaking or opening the breakable or openable part or separating the fitted part, and the container. The main body comprises a microorganism-trapping member housed in the microorganism-trapping member-side container, and the microorganism-trapping member-side container is formed by breaking or opening the breakable or opening-capable portion, or separating the fitted portion. And a microbial trapping member pressing portion for projecting the tip of the microbial trapping member from the opening and storing it again in the microbial trap member side container, and further, the cap portion Is a microorganism detecting device having a storage portion for storing the opening of the microorganism capturing member side container, and a culture medium stored or attached inside the storage portion, wherein the culture medium is A Chishirin resistant Staphylococcus aureus detection medium and a microorganism detection instrument containing a detection reagent for detecting the color change of the presence of methicillin resistant Staphylococcus aureus.

[0009]

EXAMPLES The microorganism detecting device of the present invention will be described with reference to the examples shown in the drawings. The microorganism detecting device 1 of the present invention has a container body 3 having a breakable portion 11 and a microorganism capturing member 8 housed in the container body 3, and the container body 3 captures microorganisms by the breakable portion 11. It can be divided into a member side container 3a and a cap portion 3b.
b is a storage portion 3e for storing the end portion 8a of the microorganism trapping member side container 3a after the division of the container main body 3 and the end portion 8a of the microorganism trapping member side, and detection of microorganisms stored or attached inside the storage portion 3e of the cap portion 3b. It has a culture medium 5.

Therefore, the microorganism detecting device of the present invention will be described with reference to the embodiment shown in FIG. FIG. 1 is a perspective view of an embodiment of the microorganism detecting device of the present invention, and FIG. 2 is a partially cutaway sectional view of the microorganism detecting device shown in FIG.

This microbe detecting device 1 is specifically, at least two closed portions 3c and 3d and two closed portions 3
The container body 3 having the breakable portion 11 provided between c and 3d, and the two closed portions 3c and 3 in the container body 3
The microorganism body capturing member 8 housed between d and the container body 3 is provided with the breakable portion 11 so that the microorganism capturing member 8 is exposed and the cap portion 3 is provided.
In addition to being separable into b, the cap portion 3b has an opening 3f that fits into the divided end portion 3g of the microorganism-trapping member side container 3a after division, and a storage portion 3e of the exposed portion of the microorganism-trapping member 8a. It has a medium 5 housed or attached inside the portion 3e.

The microorganism detecting device 1 includes a container body 3 and
It has a microorganism capturing member 8 (in other words, a sample collecting member 8) housed in the container body. As shown in FIG. 1 and FIG. 2, the container body 3 includes the breakable portion 1
1, the microorganism trapping member side container 3a and the cap portion 3
It is classified into b. However, before the two are divided by the breakable portion 11, the two are in a liquid-tightly joined state.

The inside of the container body 3, specifically,
The microorganism capturing member 8 is housed between the closing member 3c that forms the closing portion of the microorganism capturing member side container 3a and the closing portion 3d of the cap portion 3b. Further, the closing member 3c
Has an elastic member 9, for example, a spring, and a flat plate member 10 is fixed to the tip of the elastic member 9, and the flat plate member 10 is provided with a hole 10a. Therefore, in the microorganism detecting device 1 of this embodiment, the container body 3 includes the microorganism capturing member side container 3a and the cap portion 3
b, the closing member 3c. The container body 3 is preferably made of semi-rigid plastic, and further, at least a part of the microorganism-capturing member-side container 3a or the cap portion 3b is transparent to the extent that the internal color tone can be confirmed from the outside. It is preferable to have As such a semi-rigid plastic, polyolefins such as polypropylene and polyethylene, styrene resins, polycarbonate, etc., which have a certain degree of transparency, can be suitably used.

Further, it is preferable that the microorganism-capturing member side container 3a and the cap portion 3b are integrally formed of the above resin so that the portion which becomes the breakable portion 11 is thin. The rupturable portion 11 preferably has a wall thickness of about 0.05 to 1.0 mm, and particularly, has a thickness of 0.
1 to 0.5 mm is preferable. Alternatively, the microorganism-capturing member-side container 3a and the cap portion 3b may be formed as separate members, and a fusion-bonding portion serving as the breakable portion 11 may be formed between them. Then, the above-mentioned closing member 3C is fused to the end of the microorganism-capturing member side container 3a by ultrasonic waves or the like. Instead of the breakable part 11, it is also possible to form a pluggable part in the microorganism trapping member side container 3a and the cap part 3b.

Then, as shown in FIG. 2, the closing member 3c.
A fixed space is formed between the microorganism trapping member 8 and the closed portion 3c by the flat plate member 10 and the elastic member 9 provided in the space. As shown in FIG.
It is preferably filled with the microorganism-trapping liquid 6.
As the microorganism-trapping liquid 6, any liquid may be used as long as it does not affect the microorganisms to be captured, and depending on the microorganism, for example, physiological saline, sterile water, etc. are used. .

In order to make it easier to capture microorganisms, the microorganism-trapping liquid 6 contains a trace amount of a viscosity adjusting agent (eg, glycerin, dextrin, starch, CMC [carboxymethylcellulose], etc.). Good.
Further, the microorganism-trapping liquid 6 permeates the trapping member 8 through the outer peripheral portion of the flat plate member 10 and the holes 10a.

The microorganism-capturing member 8 is preferably a liquid-absorbent material having a certain level of liquid-absorption property, particularly a liquid-absorption property with respect to the liquid when it is filled with the above-mentioned liquid for capturing microorganisms. Is. Specifically, cotton, non-woven fabric,
Paper, a fibrous filter, a bundle of long fibers, a porous plastic, a sponge or the like can be used, and in particular, a bundle of long fibers of acetyl cellulose is suitable. The microorganism-trapping member 8 is housed in the container body 3 in a slightly compressed state, and the inner surface of the container body 3 and the outer surface of the microorganism-trapping member 8 are in close contact with each other, and there is substantially no gap. Not formed. Therefore, the microorganism-trapping liquid 6 is configured to flow out only through the microorganism-trapping member 8.

Then, the microorganism trapping member 8 is the container body 3
When is divided at the breakable portion 11, the end portion 8a of the microorganism capturing member 8 is exposed as shown in FIG. 3, and the microorganism is captured at this portion. The length of the exposed end portion 8a of the microorganism capturing member 8 is preferably about 3 to 10 mm. Then, as shown in FIG. 2, the rear end portion of the microorganism capturing member 8 is elastically supported by the flat plate member 10 and the elastic member 9 provided on the closing member 3c, and the rear end portion of the microorganism capturing member 8 is attached to the front end portion of the capturing member 8. Since the elastic member 9 expands and contracts in response to such a force, the capturing member 8 follows the deformation of the elastic member 9 and
Since it moves slightly back and forth in the axial direction of the capturing member side container 3a,
It makes the capture of microorganisms easier.

Further, it is preferable that the liquid inside the trapping member side container 3a can be discharged through the trapping member 8 by pressing the side surface. In order to do so, in the microorganism detecting device 1 of this embodiment, at least the portion of the capturing member side container 3a filled with the liquid is
It is formed of a resin having some flexibility, and is pressed into the side surface to be deformed inward. Specifically, the cross-sectional shape of the container body 3 is a rhombus,
It is preferably formed in a flat shape such as a rectangle or an ellipse, and has a side surface that facilitates pressing.
By doing this, if a liquid is required for culturing the microorganism, the filled liquid can be used,
There is no need to separately prepare a liquid for culturing. further,
The microorganisms attached (captured) to the surface (tip surface) of the capturing member 8 can be separated from the capturing member 8 by the outflow of the liquid.

Further, as described above, it is preferable to fill the inside of the container body with the liquid for trapping microorganisms. However, when the trapping member is a little hygroscopic or has a little stickiness, the It is not necessary to fill the inside of the container body with the capturing liquid.

The cap portion 3b has a closing portion 3d.
And an opening 3f that fits into the divided end 3g of the microorganism-trapping member side container 3a and the exposed portion of the microorganism-trapping member 8a.
It has a storage part 3e and a microorganism detecting medium 5 stored or attached inside the storage part 3e.

As shown in FIG. 6, the opening 3f of the cap portion 3b can be fitted with the divided end portion 3g of the microorganism-capturing member side container 3a so as to be in a substantially liquid-tight state. In addition, the exposed portion of the microorganism capturing member 8a is stored in the storage portion 3e. In this way, the opening 3f of the cap portion 3b fits with the divided end portion 3g of the microorganism-capturing member side container 3a and becomes almost liquid-tight, so that the culture can be performed in this state. Furthermore, it is preferable to use, as the material for forming at least a part of the microorganism-capturing member-side container 3a or the cap portion 3b, a material having transparency to the extent that the internal color tone can be confirmed from the outside.

In the microorganism detecting device 1 of the embodiment shown in FIG. 2, the culture medium 5 is attached to the outer surface of the closed portion 3d of the cap portion 3b, in other words, the inner surface of the storage portion 3e. As the medium 5, growth (proliferation) of microorganisms to be detected
It is preferable to use those having a nutrient source necessary for the above, and further to contain a detection reagent that changes the color tone due to the growth of the target microorganism. The content of the medium varies depending on the microorganism to be detected, but generally contains a saccharide (carbon source) and a nitrogen-containing substance. As the saccharide, glucose, lactose, sucrose, fructose, maltose, mannitol, trehalose, sorbitol and the like are used. The nitrogen-containing substance is a nitrogen source of a general culture component or an inorganic nitrogen compound as a growth factor, and amino acids, peptone, yeast extract, meat extract, tryptone, thiamine, and mixtures thereof are used. Further, as a detection reagent whose color tone changes due to the growth of a target microorganism,
Cresol red, thymol blue, blum thymol blue, phenol red, bromcresol purple,
Brilliant green, malachite green, crystal violet, water blue, neutral crimson, basic fuchsin, litmus solution, eosin Y, methylene blue, resazurin, triphenyltetrazonium chloride and the like are used. By containing such a dye,
Since the growth of microorganisms can be detected from the outside by a change in color tone, the judgment is extremely easy, and the work of adding a reagent for confirming the growth of microorganisms is unnecessary. In addition, the opening 3f of the cap portion 3b is provided with the microorganism trapping member side container 3
If the one that is fitted to the divided end portion 3g of a and is in a liquid-tight state is used, and if the detection reagent whose color tone changes due to the growth of the above microorganisms is contained in the medium, the opening portion of the cap portion 3b is formed. After fitting the end portion 3g of the microorganism-capturing member side container 3a to 3f and adding the microorganism-capturing liquid 6 to the dry medium to form a culture solution, in particular, the microorganism-capturing member-side container 3a is removed from the cap portion 3b. Since it is not necessary to remove it and the presence of the microorganism can be confirmed from the outside, the work of detecting the microorganism is extremely easy.

Further, as described above, it is preferable to preliminarily contain the detection reagent in the medium. Although the work is slightly increased, it may be added after culturing. Alternatively, the growth of microorganisms may be confirmed by increasing the turbidity of the culture solution without adding a detection reagent. Then, the medium is a liquid medium formed by adding the above-mentioned sugars, nitrogen-containing substances, and if necessary, a pigment and a pH adjusting agent to purified water, and the liquid medium thus formed is covered with the cap portion 3b. After being put in a small amount in and dried, the dry medium 5 can be attached to the inner surface of the cap portion 3b.

As the culture medium, the above-mentioned dry culture medium 5 is easy to handle and preferable, but as shown in FIG.
A removable sealing member 15 at the open end of the cap portion 3b
May be provided to hermetically seal the inside, and the liquid medium 14 may be housed inside. In particular, when the microorganism detection instrument of the present invention is applied to an MRSA detection instrument, a medium containing a carbon source such as a saccharide, a nitrogen-containing substance, and an antibiotic is used, and further, the growth of MRSA is It is preferable to contain a dye that can be detected by a change in color tone. As the saccharides, those mentioned above can be preferably used, in particular, mannitol, trehalose and the like are preferable, and as the nitrogen-containing substance, those mentioned above can be preferably used, but particularly, amino acids (especially glycine and arginine). , Urea), peptone (eg, proteol peptone), yeast extract, meat extract, tryptone, and the like, as well as mixtures thereof.

As a pigment, M
Since the culture fluid of RSA changes from neutral to acidic, specifically, it becomes acidic by the acid produced by MRSA's glycolytic ability, and therefore a dye capable of discriminating the change from neutral to acidic is used. For example, phenol red, bromcresol purple, bromthymol blue, cresol red, litmus solution, brilliant green, crystal violet and the like can be used. As the antibiotic, cefazoline, oxacillin, methicillin, cefotiam, cefetazole, cefzotim, flomoxefimipenem, fosfomycin, minocycline, vancomycin and the like can be used. Furthermore, it is preferable to add sodium pyruvate and glucose which act as a growth promoter of Staphylococcus aureus, or an energy source. Furthermore, it is preferable to add lithium chloride that acts as a growth inhibitor for suppressing the growth of bacteria other than staphylococci. Furthermore, sodium tellurite which acts as an inhibitor for suppressing the growth of bacteria other than Staphylococcus aureus,
It is preferred to add tellurates.

In the above description, the microorganism detecting device of the present invention has been described by taking an example in which MRSA is a detection target. However, the present invention is not limited to this, and various microorganisms such as Pseudomonas aeruginosa and causes of food poisoning are described. It can be used as a detection instrument for various microorganisms such as Vibrio parahaemolyticus, Clostridium botulinum, Salmonella, pathogenic Escherichia coli, Bacillus cereus, Aeromonas, enterococci, Campylobacter, Nagvibrio, and the like.

Next, an embodiment of the microorganism detecting instrument of the present invention shown in FIGS. 7 and 8 or 11 will be described with reference to the drawings. 7 is an external view of an embodiment of the microorganism detecting device of the present invention, FIG. 8 is a cross-sectional view of the microorganism detecting device shown in FIG. 7, and FIG. 11 is another example of the microorganism detecting device of the present invention. 3 is a cross-sectional view of the embodiment of FIG.

Microorganism detection instrument 2 shown in FIGS. 7 and 8.
0 is composed of a container main body divided into a microorganism trapping member side container 23 and a cap portion 25 by the detachment of the fitting part, and a microorganism trapping member 22 housed in the microorganism trapping member side container 23 of the container main body. The trapping member side container 23 is for opening the opening 23c formed by the detachment of the fitting part and for projecting the tip end of the microorganism trapping member 22 from the opening 23c and for storing it again in the microorganism trapping member side container 23. The microorganism capturing member pressing portion 23a, and the cap portion 25 has an opening 23c of the microorganism capturing member side container 23.
And a culture medium 5 stored or attached inside the storage portion.

Further, the microorganism detecting instrument 40 shown in FIG.
Is composed of a container body divided into a microorganism-capturing member-side container 23 and a cap portion 25 by breaking the breakable portion 41, and a microorganism-capturing member 22 housed in the microorganism-capturing member-side container 23 of the container body. The capturing member side container 23 is
An opening 23c formed by breaking the breakable portion, and a microorganism trapping member pressing portion 23a for projecting the tip end of the microorganism trapping member 22 from the opening 23c and storing it again in the microorganism trapping member side container 23. Further, the cap portion 25 has a storage portion for storing the opening 23c of the microorganism-capturing member side container 23, and the culture medium 5 stored or attached inside the storage portion.

The difference between the microorganism detecting instruments 20 and 40 and the detecting instrument 1 is that the detecting instruments 20 and 40 are axially expandable / contractible in the microorganism capturing member side container 23. The other configuration is substantially the same as that of the detection instrument 1, although the configuration is different. In addition, the difference between the microorganism detecting instrument 20 and the detecting instrument 40 is that the microorganism trapping member side container 23 and the cap portion 25 forming the container body are formed as separate members, or are integrally formed, and Similarly, it may be formed so as to be divided by the breakage or breakage of the pluggable part 41 (the breakable part in this embodiment), and the other is the same. Hereinafter, the detection instrument 20 will be specifically described.

As shown in FIG. 8, the microorganism-capturing-member-side container 23 of the microorganism detecting device 20 has an opening 23c at the tip for exposing the microorganism-capturing member 22 described later to a predetermined length, and the vicinity of the rear end. Has a microorganism trapping member pressing portion 23a that can expand and contract in the axial direction of the container body 23. Further, the rear end is provided with a pressing or pulling portion 23b for contracting or expanding the pressing portion 23a. In this embodiment, the pressing portion 23a is formed of an expanding and contracting portion that expands and contracts by pressing. Specifically, this expansion / contraction part is formed by a bellows dropper, and contracts in the axial direction of the container body 23 or presses or pulls by pressing the pressing or pulling part 23b provided at the rear end. It is formed so as to extend in the axial direction of the container body 23 by pulling the portion 23b. It should be noted that this bellows dropper restores its original shape by pressing or releasing the pressing of the pulling portion 23b, and the microorganism capturing member 22 to be described later automatically.
May be housed in the microorganism-trapping member side container 23. Then, in a normal state such as before use, the elastic portion 2
3a is in an expanded state as shown in FIG. Moreover, in the embodiment shown in FIG.
Although it is formed near the rear end of the container body 23, it is not limited to this and may be formed near the middle part.

The pressing portion 23a does not necessarily have to be a telescopic portion that expands and contracts by pressing, and may be, for example, a deformable portion that deforms into the microorganism trapping member side container 23 by pressing. It may be a deformable flexible portion. The pressing portion 23a is the pressing portion 23a.
By releasing the pressing of the, the original shape is restored, and the microbial trapping member 22 is automatically moved to the microbial trapping member side container 2
It is preferable that it is housed in 3.

The pressing or pulling portion 23b is preferably formed of a material or a wall thickness that does not deform even when pressed or pulled. In this embodiment, since the microorganism trapping member side container 23 is integrally molded, the pressing or pulling portion 23b is formed thicker than the other portions. Further, as shown in FIG. 8, it is preferable that a region 23e for fixing a rear end portion of a capturing member 22 described later is formed on the inner surface of the pressing or pulling portion 23b. Furthermore, it is preferable that a convex portion 23d is provided on the outer surface of the tip portion of the microorganism capturing member side container 23. This can prevent the cap portion 25 from easily coming off when fitted to the rear end opening 25b of the cap portion 25, which will be described later.

As shown in FIG. 8, the microorganism trapping member 22 is housed inside the microorganism trapping member side container 23. The capturing member 22 is a microorganism (for example, MRSA).
In this embodiment, when the microorganisms are captured, the contraction of the pressing portion 23a causes the inside of the microorganism capturing member side container 23 to move in the distal direction to expose the microorganisms for a predetermined length through the opening 23c, thereby removing the microorganisms. After being captured, the pressing portion 23a is extended to move toward the rear end direction of the microorganism-capturing member-side container 23 and remain in the microorganism-capturing member-side container 23 after capturing. As described above, in the microorganism detecting device 20 of the present invention, since the trapping member 22 can be left in the microorganism trapping member side container 23 by extending the pressing portion 23a, the detection target site (for example, human skin). , Clothes, floor, nasal cavity, hospital wall, bed, etc.), after capturing the microorganisms, when inserting the cap portion 25 into the microorganism capturing member side container 23, the microorganisms do not adhere to the fingers, In addition, there is less risk of infectious diseases coming into contact with the patient with contaminated fingers. Further, since the microorganisms and the like attached to the hand adhere to the capturing member 22 and the captured microorganisms adhere to the hand on the contrary, an accurate inspection result can be obtained.
By contracting the pressing portion 23a, the capturing member 22 moves in the tip direction in the microorganism capturing member side container 23, and the length exposed from the opening 23c [(a) shown in FIG. 9] is About 3 to 10 mm is suitable.

In order to move the trapping member 22 in the axial direction of the microorganism trapping member side container 23 as the pressing portion 23a expands and contracts, the trapping member 22 of this embodiment has its rear end in the above-mentioned region 23e. It is fixed. However, the configuration in which the capturing member 22 is moved along with the expansion and contraction of the pressing portion 23a is not limited to this. For example, as described above, the pressing portion 2 may be moved.
3a may be formed near the middle portion of the microorganism-capturing member side container 23 and fixed to the inner surface of the cylindrical portion provided near the rear end portion.

Further, in the microorganism trapping member side container 23 (in this embodiment, the pressing portion 23a and the intermediate portion), FIG.
As shown in, it is preferable that the microorganism-trapping liquid 24 is filled. As the microorganism-trapping liquid 24, any liquid may be used as long as it does not affect the microorganisms to be trapped, and depending on the microorganism, for example, physiological saline, sterile water, etc. are used. . Further, in order to make it easier to capture the microorganisms, the microorganism-trapping liquid 24 may contain a slight amount of a viscosity adjusting agent (eg, glycerin, dextrin, starch, CMC [carboxymethylcellulose], etc.).

It is to be noted that the microorganism-capturing member 22 preferably has a certain degree of liquid-absorption property, particularly when it is filled with the above-mentioned liquid for capturing microorganisms, it has a high liquid-absorption property and is inactive. Is. Specifically, cotton, non-woven fabric,
Paper, a fibrous filter, a bundle of long fibers, a porous plastic, a sponge or the like can be used, and in particular, a bundle of long fibers of acetyl cellulose is suitable. The tip of the trapping member 22 of the microorganism trapping member 22 is in close contact with the inner surface near the tip formed to have a small diameter. Therefore, the microorganism-trapping liquid 24 is configured to flow out only through the microorganism-trapping member 22.

The microorganism-capturing liquid 24 can flow out through the trapping member 22 because the internal volume of the microorganism trapping member side container 23 decreases due to the deformation of the pressing portion 23a.
It becomes Noh . Therefore, when a liquid is required for culturing the microorganism, the filled liquid can be used, and it is not necessary to separately prepare a liquid for culturing. Furthermore, the microorganisms attached (captured) to the surface (tip surface) of the capturing member 22 are
It is also possible to separate it from the capturing member 22 by the outflow of the liquid. Although it is preferable to fill the inside of the microorganism-trapping member side container 23 with the microorganism-trapping liquid 24 as described above, when the capturing member 22 is slightly hygroscopic, or has a slight adhesive property, etc. Does not need to fill the inside of the microorganism-capturing member side container 23 with the microorganism-trapping liquid 24.

Further, as shown in FIG. 8, in the cap portion 25, the rear end opening 25b is fitted with the opening 23c of the microorganism trapping member side container 23 to maintain a substantially liquid-tight state and is detected. It also functions as a medium for microorganisms. Therefore, the cap portion 25 has a rear end opening 25b that fits into the front end portion of the microorganism trapping member side container 23, and also has the medium 5 stored or attached inside. Then, the microorganism detecting device 20 of this embodiment is similar to the detecting device 1 in that the cap portion 25 is inverted before and after use and is fitted into the opening portion 23c of the microorganism capturing member side container 23. The portion 25 has a tip end opening 25 a that fits with the tip end of the microorganism-trapping member side container 23. Further, the rear end opening 25b and the front end opening 25
A closed portion 25d is formed between the a and the a to make them incapable of communicating with each other, and the culture medium 5 has a closed portion 2d as shown in FIG.
It is housed or attached to the tip opening 25a side from 5d. Then, the cap portion 25, as shown in FIG.
It is preferable that the medium storage portion 26 for storing the culture medium 5 is formed, and that the inner cylinder portion 25c that can be inserted into the opening portion 23c of the microorganism capturing member side container 23 is provided. By forming the inner cylinder portion 25c, microorganisms can easily move from the capturing member 22 to the medium 5.

The microorganism trapping member side container 23 is preferably formed of semi-rigid plastic having a certain degree of flexibility. Furthermore, it is preferable that at least a part of the microorganism trapping member side container 23 or the cap portion 25 has transparency to the extent that the internal color tone can be confirmed from the outside, and the cap portion 25 is the microorganism trapping member side container. The material may be the same as that of the container 23 or may be harder than the material of the container 23. As for plastic,
Polyolefins such as polypropylene and polyethylene, vinyl chloride resins, styrene resins, and polycarbonates, which have a certain degree of transparency, can be preferably used. Moreover, as the culture medium 5, the one used for the description of the microorganism detection instrument 1 described above can be preferably used. Further, as the medium 5 when the microorganism detecting device 20 of the present invention is applied to the MRSA detecting device, the above-mentioned MRSA detecting medium can be preferably used.

The medium 5 is a liquid medium formed by adding the above-mentioned saccharides, nitrogen-containing substances, and if necessary, pigment and pH adjusting agent to purified water, and the liquid medium thus formed. The medium storage portion 2 of the cap portion 25
By putting a small amount in 6 and then drying, the dry medium 5 can be attached to the inner surface of the cap portion.

Next, regarding the action of the microorganism detecting instrument of the present invention, an example in which the microorganism detecting instrument of the present invention is applied to an MRSA detecting instrument is used, and further, referring to FIG. 1, FIG. 4, FIG. 5 and FIG. explain. First, a force such as bending at the rupturable portion 11 portion of the microorganism detecting device 1 of FIG. 1 is applied to the container body 3, and as shown in FIG. A catching member side container 3a,
It is divided into a cap portion 3b. As a result, the end portion 8 of the microorganism capturing member 8 is more
a is exposed to the outside. In the microorganism detecting device 1, the microorganism capturing member side container 3a is filled with the microorganism capturing liquid 6 and the capturing member 8 is made of a liquid absorbing material. Therefore, the capturing member 8
Is in a wet state by absorbing the capturing liquid 6.

Then, as shown in FIG. 5, the surface of the detection target portion 21 (for example, human skin, clothes, nose, wall of the hospital room, bed, etc.) is the surface of the end 8a of the microorganism capturing member 8. A sample is collected by the capturing member 8 by wiping.
Then, the end portion of the microorganism capturing member 8 is slightly inserted into the opening 3f side of the cap portion 3b, and in this state, the side surface of the microorganism capturing member side container 3a is pressed. Thereby, the container 3
The liquid in a flows into the cap portion 3b, and microorganisms attached to the surface of the capturing member 8a (for example, MRS).
A) also moves to the cap portion 3b. Then, the dried culture medium 5 attached to the surface of the cap portion 3b is dissolved in the introduced microorganism-trapping liquid 6 to become a liquid culture solution. Then, the end 3g of the microorganism-capturing-member-side container 3a is fitted into the opening 3f of the cap 3b. As a result, the culture solution does not flow out. In addition, since the medium used in the microorganism detecting instrument (MRSA detecting instrument) of this example contains a detection reagent (for example, phenol red), the culture solution 5a corresponds to the detection reagent. It has a color (red if phenol red). Since there is a possibility that the dry medium is not completely dissolved or dispersed in the culture medium 5a, the container is shaken slightly while the capturing member side container 3a and the cap portion 3b are fitted, Dissolve the medium completely in. By this operation, the microorganisms attached to the surface of the microorganism capturing member 8 also move into the liquid.

Then, necessary conditions (for example, 35 ° C., 2
Incubate for about 4 hours. Then, since the detection reagent is contained in the medium used in the microorganism detecting instrument (MRSA detecting instrument) of this example, if the microorganism grows in the detecting instrument, It decomposes sugar during growth, and the acid produced by this decomposition causes
Since the culture solution changes from neutral to acidic, for example, when the culture solution contains phenol red, the color tone changes from red to yellow. This allows MRS from the outside.
The presence of A can be easily confirmed. Also, if
When the detection reagent is not added to the medium, the culture solution is not spilled, the capturing member side container 3a is removed from the cap portion 3b, and the detection reagent is added to the culture solution.
The color confirms the presence of microorganisms.

Next, the operation of the microorganism detecting instrument of the present invention shown in FIGS. 7 to 10 will be described using an example (detecting instrument 20) applied to an MRSA detecting instrument. First, the cap portion 25 fitted to the microorganism capturing member side container 23 is removed, and the pressing or pulling member 23b provided at the rear end of the microorganism capturing member side container 23 is pressed to shrink the pressing portion 23a, As shown in FIG. 9, the capturing member 22 is exposed for a predetermined length through the opening 23c. The inside of the microorganism trapping member side container 23 is filled with the trapping liquid 24, and the trapping member 8 is formed of a material having a liquid absorbing property. Liquid 2
It has absorbed 4 and is in a wet state. Then, the surface of the detection target site (for example, human skin, clothes, nose, wall of the hospital room, bed, etc.) is wiped with the surface of the end of the capturing member 22, and a sample is collected by the capturing member 22. To do.

Then, the pressing or pulling portion 23b is gripped and pulled in the axial rear end direction of the microorganism-trapping member side container 23, so that the trapping member 22 is placed inside the microorganism-trapping member side container 23 as shown in FIG. Detain. As described above, in the microorganism detecting device 20 of the present invention, since the capturing member 22 can be left in the microorganism capturing member side container 23 after the sample is collected, the detection target site (clothes, floor, nasal cavity, etc.) is wiped off. During the work of inserting the cap into the cap portion 25 after capturing, the microorganisms do not adhere to the fingers, and there is little risk of contacting the patient with the contaminated fingers and causing an infection. Further, since the microorganisms and the like attached to the hand adhere to the capturing member 22 and the captured microorganisms adhere to the hand on the contrary, an accurate inspection result can be obtained.

Then, as shown in FIG. 10, the inner cylindrical portion 25c of the cap portion 25 is inserted into the opening 23c of the microorganism-trapping member side container 23, and the cap portion 25 is fitted into the microorganism-trapping member side container 23. . Then, in this state, the pressing portion 23a is expanded or contracted, or the microorganism capturing member side container 23
Press the side surface (near the middle part) of. This allows the container 2
The liquid 24 in the liquid 3 flows into the medium storage portion 26 of the cap portion 25. Then, the introduced microorganism-trapping liquid 24
As a result, the dry medium 5 attached to the surface of the inner tubular portion 25c
Dissolves into a liquid culture medium. In addition, since the medium 5 used in the microorganism detection instrument 20 (MRSA detection instrument) of this embodiment contains a detection reagent (for example, phenol red), the culture solution corresponds to the detection reagent. Shows the color (red if phenol red). Since there is a possibility that the dry medium is not completely dissolved or dispersed in the culture solution, the container is shaken slightly in the state where the microorganism-capturing member side container 23 and the cap portion 25 are fitted to each other. Dissolve the medium completely in. Further, by this work, the microorganisms adhering to the surface of the microorganism capturing member 22 also easily move into the liquid.

Then, necessary conditions (for example, 35 ° C., 2
Incubate for about 4 hours. In addition, in this medium 5,
Since the detection reagent is contained, if a microorganism grows in the detection instrument 20, sugar is decomposed during the growth of the microorganism, and the acid produced by the decomposition causes the culture solution to be, for example, The culture fluid changes from neutral to acidic.
When phenol red is contained, the color tone changes from red to yellow. As a result, the presence of MRSA can be easily confirmed from the outside.

[0050]

EFFECTS OF THE INVENTION The microorganism detecting device of the present invention comprises a container body having a breakable portion and a microorganism capturing member housed in the container body, wherein the container body allows the microorganisms to be separated by the breakable portion. The capturing member side container and the cap portion are separable, and further, the cap portion is, after the division, a storing portion for storing a microorganism capturing member side end portion of the microorganism capturing member side container, and inside the storing portion. Since it has a medium that is stored or attached, this detection instrument can capture microorganisms and culture microorganisms, and without using many instruments for the detection of microorganisms, The detection work can be performed extremely easily.

Further, the microorganism detecting device of the present invention has a container body having a breakable portion and a microorganism capturing member housed in the container body, wherein the container body is provided with the microorganisms by the breakable portion. The capturing member side container and the cap portion are separable, and further, the cap portion is, after the division, a storing portion for storing a microorganism capturing member side end portion of the microorganism capturing member side container, and inside the storing portion. It has a medium for detecting microorganisms stored or attached thereto, and the medium is M
Since it is a medium for detecting RSA, and contains a detection reagent whose color tone is changed by the growth of MRSA, this detection instrument captures MRSA and cultures MRSA.
Furthermore, the proliferation of MRSA, that is, the existence thereof can be confirmed, and the detection operation can be performed extremely easily without using many instruments for detecting MRSA.

Further, the microorganism detecting device of the present invention comprises a container body divided into a microorganism-capturing member side container and a cap part by breaking or opening the breakable or openable part or separating the fitted part, and the container. The main body comprises a microorganism-trapping member housed in the microorganism-trapping member-side container, and the microorganism-trapping member-side container is formed by breaking or opening the breakable or opening-capable portion, or separating the fitted portion. And a microbial trapping member pressing portion for projecting the tip of the microbial trapping member from the opening and storing it again in the microbial trap member side container, and further, the cap portion Is a storage unit for storing the opening of the microorganism-capturing member side container, and a medium stored or attached inside the storage unit,
Unlike conventional methods, it is extremely easy to detect microorganisms without using many instruments, and there is less possibility that microorganisms will adhere to fingers and cause infections, and more accurate The test result can be obtained.

[Brief description of drawings]

FIG. 1 is a side view of a microorganism detecting instrument according to an embodiment of the present invention.

FIG. 2 is a partially cutaway sectional view of the microorganism detecting device shown in FIG.

FIG. 3 is a partially cutaway sectional view of a microorganism detecting device according to another embodiment of the present invention.

FIG. 4 is an explanatory view for explaining the action of the microorganism detecting instrument of the present invention.

FIG. 5 is an explanatory view for explaining the action of the microorganism detecting instrument of the present invention.

FIG. 6 is an explanatory view for explaining the action of the microorganism detecting instrument of the present invention.

FIG. 7 is an external view of an embodiment of the microorganism detecting instrument of the present invention.

FIG. 8 is a cross-sectional view of the microorganism detecting device shown in FIG. 7.

FIG. 9 is an explanatory diagram for explaining the operation of the microorganism detecting device shown in FIG. 7.

FIG. 10 is an explanatory diagram for explaining the operation of the microorganism detecting device shown in FIG. 7.

FIG. 11 is a cross-sectional view of another embodiment of the microorganism detecting device of the present invention.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Microorganism detection instrument 3 Container main body 3a Microorganism capture member side container 3b Cap part 3c Closing member 3e Microorganism capture member side storage section 5 Medium 6 Microorganism capture liquid 8 Microorganism capture member 11 Breakable portion 20 Microorganism detection instrument 22 Microorganism capture member 23 Microorganism capturing member side container 23a Pressing portion 23b Pressing or pulling portion 23c Opening portion 24 Microbial capturing liquid 25 Cap portion 25a Tip opening portion 25b Rear end opening portion 25c Inner tube portion 25d Closing portion 26 Medium storing portion 40 Microorganism detection instrument 41 Breaking Or openable part

Claims (1)

[Claims] 1. A container main body having a breakable portion, and a microorganism capturing member housed in the container main body, wherein the container main body has a microorganism capturing member side container and a cap portion by the breakable portion. Further, the cap portion has a storage portion for storing the end portion of the microorganism trapping member side container of the microorganism trapping member side after the division, and a culture medium stored or attached inside the storage portion. Microbial detection instrument characterized by being.