JPH0660818U - Optical microscope - Google Patents

Abstract

(57) [Abstract] [Purpose] To obtain an optical microscope having high rigidity that can sufficiently reduce vibration even when a heavy object such as a manipulator is attached to a mirror base. [Structure] A heavy object support unit 1 for supporting a heavy object such as a manipulator on a mirror base 101 for attaching an eyepiece lens 109, an objective lens 107, a sample table 106 and the like.
02 is integrally formed at a position close to the sample table 106. An optical microscope.

Description

[Detailed description of the device]

[0001]

[Industrial applications]

 The present invention relates to an optical microscope, and particularly to an optical microscope optimal for performing micromanipulation of cells in fields such as cell biology.

[0002]

[Prior art]

 In the field of cell biology and the like, conventionally, micromanipulators (hereinafter simply referred to as “manipulators”) have been used for micromanipulation of biological cells. There are first, second, and third examples described below as methods of attaching the manipulator to the optical microscope at this time.

FIG. 5 is a diagram showing a first conventional example, in which the manipulator 1 is supported by a magnet stand S composed of a magnet 2 and a column 3, and the stand S is attracted to a surface plate 20 by the magnet 2. This is a fixed example. The manipulator 1 includes a posture adjusting attachment arm 4 that can move vertically and horizontally with respect to the support column 3, an operation portion 4a, a position fixing portion 4b, a Z-axis fine movement arm support portion 5, and a Z-axis fine movement amount. An operating unit 5a for operating, a Z-axis fine moving arm 6, a Y-axis operating unit 6a for operating the Y-axis fine moving amount, a Y-axis fine moving arm 7, and an X-axis operating unit 7a for operating the X-axis fine moving amount. , An X-axis fine movement arm 8, an operation member attachment portion 9, and a cell operation member 104 including a needle, a micropipette, an electrode and the like.

The microscope 13 includes a mirror base 101, a sample table 106, a petri dish 105 containing a sample 12, an objective lens 107, a revolver 18, an eyepiece 109, a lens barrel 110, and a revolver vertical movement. It comprises a knob 16 and a revolver vertical movement mechanism 19 (not shown).

In such a structure, the observer can move the cell manipulating member 104 by manipulating the manipulating units 4a, 5a, 6a and 7a while looking into the eyepiece lens 109, whereby the petri dish can be moved. The sample 12 in 105 can be manipulated.

FIG. 6 is a diagram showing a second conventional example, which is an example in which an electric manipulator 200 is attached to the sample stage 106. The manipulator 200 includes a mounting member 201, an X-axis actuator 202x, a Y-axis actuator 202y, a Z-axis actuator 202z, and a cell manipulating member 104. In the case of the example of FIG. 6, each of the actuators 202x, 202y and 202z is moved to a desired position by the observer controlling a controller (not shown), and the cell manipulating member 104 is moved accordingly. be able to.

FIG. 7 is a diagram showing a third conventional example, (a) is a side view, and (b) is a rear view. This is an example in which a manipulator is attached to a support member 301 attached by a screw 302 to a mirror base 101 of an inverted microscope. That is, tap holes 304 are formed in at least two positions of the mirror base 101, and screws 302 are detachably screwed into the tap holes 304 to fix the support member 301 to the mirror base 101. A fine movement mechanism 103 is placed on 301, and is detachably fixed by a screw 303 so that the cell manipulation member 104 is finely moved by the fine movement mechanism 103. Incidentally, 111 is a lamp house for transmitted illumination, and 112 is a column for lamp house.

[0008]

[Problems to be solved by the device]

 In the example of FIG. 5, the magnet stand S including the magnet 2 and the column 3 is fixed at a position apart from the mirror base 101. Therefore, the length from the magnet stand S to the tip of the cell manipulating member 104 becomes long, the rigidity of the entire manipulator 1 from 2 to 9 and 104 becomes low, and the cell manipulating member 104 easily vibrates accordingly. Become. It goes without saying that the vibration of the cell manipulating member 104 hinders the manipulation.

In the example of FIG. 6, the manipulator is attached to the sample table 106, but it is generally assumed that the sample table 106 mounts a lightweight sample. Therefore, similar to the example of FIG. The rigidity is low. In addition, since the actuator is an electric type, it is much heavier than a manual type or a manual hydraulic type manipulator, so that it is more likely to vibrate.

In the example of FIG. 7, the support member 301 is detachably attached to the mirror base 101 with the screw 302, and the fine movement mechanism 103 is detachably attached to the support member 301 with the screw 303. Therefore, the rigidity is lowered. The presence of such a detached portion generally brings about a decrease in rigidity regardless of a fixing means such as a screw or a clamp.

The present invention has been made under such circumstances, and an optical microscope having high rigidity that can sufficiently reduce vibration even when a heavy object such as a manipulator is attached to a mirror base. The purpose is to provide.

[0012]

[Means for Solving the Problems]

 In order to solve the above-mentioned object, a device corresponding to claim 1 is a mirror base for mounting an eyepiece lens, an objective lens, a sample table, and the like, and a heavy-weight support portion for supporting a heavy load such as a manipulator. The optical microscope is characterized by being integrally formed at a position close to the sample table.

[0013]

[Action]

 According to the optical microscope of the present invention corresponding to claim 1, since the heavy object support is integrally formed at a position close to the sample base of the mirror base, the heavy object such as a manipulator is attached to the heavy object support. It is possible to realize a high-rigidity mirror substrate that can sufficiently reduce vibration even when supporting the mirror.

[0014]

【Example】

 Hereinafter, embodiments of the present invention will be described with reference to the drawings. FIG. 1 is a view for explaining a first embodiment of the present invention, (a) is a side view and (b) is a rear view. A feature is that a heavy object support portion 102 for supporting a heavy object such as a manipulator is integrally formed on a mirror base 101 of an inverted optical microscope at a position close to a sample table 106.

The heavy load supporting portion 102 has a flat plate shape, is formed on the back side 101 b of the mirror base 101 (the side opposite to the front side 101 a where the eyepiece lens 109 is located), and is formed on each of the left and right sides. ing. The fine movement mechanism 103 of the manipulator is placed on one side of the heavy load supporting unit 102 (left side in FIG. 1A), and the bottom face side of the heavy load supporting unit 102 is detachably supported by the screw 303 inserted. ing. Except for the points described above, the configuration is the same as that of the conventional example of FIG.

According to the first embodiment described above, since the heavy load supporting portion 102 is formed integrally with the mirror base 101, the supporting member 301 is attached to the mirror base 101 as in the conventional example of FIG. On the other hand, the rigidity is much higher than that of attachment by the screw 302. Further, since the heavy load supporting portion 102 is arranged at a position close to the sample table 106, the length from the attachment portion of the manipulator to the tip of the cell manipulating member 104 can be shortened. Furthermore, the only detachment point that causes the reduction in rigidity is the attachment point by the screw 303. As a result, the rigidity of the entire device, including the microscope and manipulator, can be increased, and vibration can be suppressed to a low level.

Further, even if the fine movement mechanism 103 is not provided and a heavy manipulator having an electric actuator as shown in FIG. 6 is provided instead, the rigidity can be provided so that the vibration is suppressed. Needless to say, the same effect can be obtained regardless of the mounting position of the fine movement mechanism 103 or the like on any position of the heavy load supporting portion 102.

FIG. 2 shows a second embodiment of the present invention, in which (a) is a front view and (b) is a side view. This embodiment is the same as the embodiment of FIG. 1 except that the heavy load supporting portion 102 is integrally formed on the front side 101a of the mirror base 101.

With this configuration, when the manipulator operation unit is provided in the fine movement mechanism 103, since the operation unit is located in the hand of the observer, the operability is significantly improved. . On the other hand, in the case of the embodiment shown in FIG. 1, since the heavy load supporting portion 102 is formed on the back side of the mirror base 101, the operability for the observer is poor. The other effects are the same as those of the embodiment shown in FIG.

FIG. 3 shows a third embodiment of the present invention, (a) is a plan view and (b) is a side view. This embodiment is different from the embodiments of FIGS. 1 and 2 in that the heavy weight supporting portion 102 is integrally formed so as to bridge the front side 101a and the back side 101b of the mirror base 101. .

With such a configuration, the selection range of the attachment position of the heavy object such as the manipulator is widened, and it is possible to cope with various purposes. In addition, since the mirror base 101 and the weight supporting portion 102 form a square shape with the gate closed, the rigidity of the mirror base 101 is remarkably improved as compared with the embodiments of FIGS. 1 and 2 described above. To do.

FIG. 4 is a perspective view showing a fourth embodiment of the present invention. In FIG. 4, a heavy object support portion 102 is integrally formed on a mirror base 101 of an upright microscope. , Different from the embodiment of FIGS. 2 and 3. In this embodiment, the same effect as the embodiment of FIG. 1 can be obtained.

[0023]

[Effect of device]

 According to the present invention described above, it is possible to provide an optical microscope having high rigidity such that vibration can be sufficiently reduced even when a heavy object such as a manipulator is attached to a mirror base.

[Brief description of drawings]

1A and 1B are a side view and a rear view showing a first embodiment of an optical microscope of the present invention, respectively.

2 (a) and 2 (b) are respectively a front view and a side view showing a second embodiment of the optical microscope of the present invention.

3 (a) and 3 (b) are respectively a plan view and a side view showing a third embodiment of the optical microscope of the present invention.

FIG. 4 is a perspective view showing a fourth embodiment of the optical microscope of the present invention.

FIG. 5 is a perspective view showing a first example of a conventional optical microscope.

FIG. 6 is a perspective view showing a second example of a conventional optical microscope.

7A and 7B are a side view and a rear view, respectively, showing a third example of the conventional optical microscope.

[Explanation of symbols]

101 ... Mirror base, 102 ... Heavy object support section, 103 ... Fine movement mechanism, 104 ... Cell manipulation member, 106 ... Sample stage, 107 ...
Objective lens, 109 ... eyepiece lens, 303 ... screw.

Claims (1)

[Scope of utility model registration request] 1. A heavy-weight support portion for supporting a heavy object such as a manipulator is integrally formed on a mirror base for attaching an eyepiece lens, an objective lens, a sample table, etc. at a position close to the sample table. An optical microscope characterized by the above.