JPH0639317A - Liquid pressure monitoring device of elutriator cell separation system - Google Patents

Abstract

PURPOSE:To make a measuring unit completely independent of a pipe so that the pipe can be connected to an autoclave and make it possible to stop feeding of liquid automatically when an abnormal pressure has occurred by measuring the pressure of fluid in the pipe in noncontact manner. CONSTITUTION:A feed pipe 2 and an exhaust pipe 3 are connected to a bottle 1. The pipe 2 is arranged so as to reach the upper part of the bottle 1, while the pipe 3 is arranged so as to reach the bottom thereof. An end of the pipe 2 is connected to a beaker 7 through a liquid feed pump 5 and an end of the pipe 3 is connected to a system 8. All of these connections are performed in airtight condition. Further, an electrostatic capacity switch 4 is provided near the bottle 1. The switch 4 is connected to the pump 5.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a hydraulic pressure monitoring device for an Eltriator cell separating apparatus for separating cells by size by utilizing centrifugal force and flow force.

[0002]

2. Description of the Related Art FIG. 2 shows a conventional hydraulic pressure monitoring device. In the Eltriator cell separation system, there is a part that feeds the inside of the pipe. The liquid is fed to the system main body 8 by the liquid feeding pump 5 sucking up the liquid contained in the beaker 7 and the piping 9. If the pressure of liquid transfer is high, the connection of the pipe may be disconnected.
Therefore, the conventional technique is to install a pressure gauge 6 in the middle of the pipe 9 to measure the pressure of the liquid flowing in the pipe, and visually check whether the value is always within a normal pressure range. The pump 5 was stopped manually.

[0003]

In order to handle cells in the Eltriator cell separation system, all piping must be sterilized. Sterilization is generally performed in an autoclave (put in a device that creates conditions of 1.2 atm, 121 ° C., 20 minutes), but in the conventional one, the pressure gauge 6 is connected to the pipe 9 so that the pressure gauge is Every six had to be autoclaved. Since the pressure gauge 6 is made of metal, it rusts and breaks down when applied to an autoclave, so that sterilization by the autoclave is practically impossible.

In addition to this, there have been troublesome operations such as the pressure gauge 6 must be constantly monitored and the liquid feed pump 5 must be manually stopped when an abnormality occurs.

The object of the present invention is to completely separate the pipe part and the pressure measuring part in order to enable autoclave sterilization, and to automatically monitor the pressure of the liquid feed and to automatically feed the liquid when an abnormality occurs. It is to stop.

[0006]

[Means for Solving the Problems] The above object is to provide a bottle in the middle of a pipe to form a liquid reservoir, and to make a liquid phase and a gas phase in the bottle. It can be achieved by measuring the capacitance of the liquid phase from the outside of the bottle by using a capacitance sensor without contact with the liquid phase by utilizing the change with pressure change. This can also be achieved by using an electrostatic capacity switch as an electrostatic capacity sensor and stopping the pumping of liquid when an abnormal pressure is reached.

[0007]

The operation is shown in FIG. Prepare the liquid in the beaker 7 and the bottle 1 in advance. The liquid prepared in the beaker 7 is once introduced into the bottle 1 from the introduction pipe 2 by the liquid feeding pump 5. Since the bottle 1 is sealed, the same amount of liquid as the introduced liquid flows through the discharge pipe 3 to the system main body. When the pressure of the flowing liquid increases, the amount of the liquid stored in the bottle 1 increases and the capacitance of the liquid stored in the bottle 1 changes.
This change amount can be sensed in a non-contact manner with the liquid by the capacitance switch 4 on the outside of the bottle, and when the abnormal pressure is reached, the liquid feed pump 5 can be turned off to stop the liquid feed.

[0008]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS FIG. 1 shows the structure of the present invention. An introduction pipe 2 and a discharge pipe 3 are connected to the bottle 1 to the bottle 1. The pipe 2 reaches the upper part of the bottle 1, and the pipe 3 reaches the bottom of the bottle 1. The other end of the introduction pipe 2 is connected to the beaker 7 via the liquid feed pump 5, and the other end of the discharge pipe 3 is connected to the system body 8. Make all these connections in a sealed manner. Further, a capacitance switch 4 is provided near the bottle 1. This switch is connected to the liquid feed pump 5.

[0009]

According to the present invention, the pipe section and the pressure measuring device can be completely independent, and autoclave sterilization is possible. In addition, it automatically monitors the fluid pressure and automatically stops liquid delivery in the event of an abnormality.

[Brief description of drawings]

FIG. 1 is a pressure monitoring device according to the present invention.

FIG. 2 is a conventional pressure monitoring device.

[Explanation of symbols]

1 is a bottle, 2 is an introduction pipe, 3 is a discharge pipe, 4 is a capacitance switch, 5 is a liquid feed pump, 6 is a pressure gauge, 7 is a beaker, 8 is a system body, and 9 is a pipe.

Claims (2)

[Claims] 1. In a pipe for sending a liquid using a pump,
A liquid reservoir is provided in the middle with a resin-sealed bottle.When connecting the bottle and the pipe, both the pipe for pouring the liquid into the bottle and the pipe for discharging the liquid are introduced from the top of the bottle into the bottle and the liquid is poured. The pipe extends to the top so that it does not touch the liquid accumulated in the bottle, and the pipe that discharges the liquid extends to the bottom so that it is always immersed in the liquid, and the capacitance of the liquid in this bottle changes with the pressure change of the liquid sending. By utilizing this, the capacitance of the liquid is measured from the outside of the bottle using a capacitance sensor without contacting the liquid, and the pressure of the liquid in the pipe is sensed. Liquid pressure monitoring device for separation system. 2. The fluid pressure monitoring device of the eltriator cell separation system according to claim 1, wherein a capacitance switch is used as the capacitance sensor, and the liquid feed pump can be controlled by this switch.