JPH0634684Y2 - Cooled electrophoresis device - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial field of application] The present invention relates to an improvement of an electrophoresis apparatus including an upper buffer tank, a lower buffer tank, and a gel glass plate, mainly including cooling means.

[Conventional technology]

The smileing effect in electrophoresis is a phenomenon that occurs because the temperature at the center of the glass plate becomes higher than the temperatures at the left and right during migration, and the temperature difference causes the sample in the center of the gel to migrate fastest.

This smiley effect has always made it difficult to analyze the data during electrophoresis of nucleic acids and proteins. Then, in the conventional electrophoretic device, in order to avoid this smile effect, cooling water is circulated around the glass plate to make the temperature of the glass plate uniform.

[Problems to be solved by the invention]

However, it only complicates the mechanism of the device unnecessarily, and the size of the glass plate, that is, the migration distance cannot be changed depending on the application.

The present invention has been made to solve the above-mentioned drawbacks, and an object of the present invention is to provide a simple cooling-type electrophoretic device that prevents the smile effect.

[Means for solving problems]

Briefly describing the present invention, the present invention relates to a cooling type electrophoretic device, which is an electrophoretic device including an upper buffer tank, a lower buffer tank and a gel glass plate, wherein the upper buffer tank is the lower buffer tank. From the top to the top of the device, the back glass of the gel glass plate constitutes the front part of the upper buffer tank, and the gel glass plate is adhered to form the upper buffer tank, and the buffer is formed. It is characterized in that the liquid can directly directly contact a portion of the back surface glass of the gel glass plate corresponding to substantially the entire gel portion and can always communicate with the upper portion of the gel in the gel glass plate. .

In the present invention, in order to realize the above-mentioned characteristics, it is necessary to bring the upper buffer tank and the gel glass plate into close contact with each other so that the buffer solution does not leak and short-circuit with the lower buffer tank.

On the other hand, considering cleaning of the buffer tank and glass plate, replacement of the buffer solution, etc.
It is preferable that a packing having excellent elasticity and corrosion resistance, such as synthetic rubber, is provided between the both, and it is detachable so as to be stopped by the W clip.

Moreover, it is preferable that the length of the upper buffer tank is vertically long according to the longest height of the electrophoresis apparatus, and on the other hand, two or more gel glass plates having different sizes, that is, different heights are provided. With such a configuration, a gel glass plate according to a desired migration distance can be selected, and only by exchanging the gel glass plate, the migration distance can be achieved without changing the size and position of the upper buffer tank. Can be adjusted.

Furthermore, when performing electrophoresis for a long time, the buffer solution may evaporate from the upper part of the upper buffer tank, and the buffer solution may decrease. In the present invention, in order to prevent it,
Liquid may be supplied from time to time, but one or more holes are provided at the upper position corresponding to the desired migration distance on the plate surface of the constituent material of the upper buffer tank, and the buffer liquid supply tank is attached correspondingly to it.
A structure in which the buffer solution sequentially flows through the holes is also suitable.

In consideration of cleaning and liquid exchange, it is preferable that this replenishment tank be detachable such as screwed or W-clipped. Also, when the migration distance is long, unnecessary holes at the bottom should be stopped. The structure should be such that the liquid does not leak at the stopper.

Other parts of the device of the present invention may be as usual device,
For example, the lower buffer tank, the power source, etc. do not change, and the upper buffer tank may stand alone on the lower buffer tank, or may be held upright by a column.

〔Example〕

Hereinafter, the present invention will be described in more detail with reference to Examples.
The present invention is not limited to these embodiments.

FIG. 1 is a perspective view of a portion related to a buffer tank of one example of the device of the present invention, and FIG. 2 is a perspective view of a portion related to an upper buffer solution replenishing tank of another example of the device of the present invention. At
Reference numeral 1 is an upper buffer tank, 2 is a packing, 3 is a lower buffer tank, 4 is a gel glass plate, 5 is a groove in the lower buffer tank, 6 is an upper buffer solution replenishing tank, and 7 is a hole.

In each of the above figures, a common electrode terminal, a stopper,
Equipment such as packing of glass plates and columns used as necessary are omitted. Further, the structure of the back glass of 4 for making the upper buffer liquid and the upper part of the gel communicate with each other is not limited to the one shown in FIG. 1, and, for example, as usual, the back glass is simply made lower than the front glass in height. Alternatively, a structure may be adopted in which the upper part of the whole glass and the part 2 are brought into close contact with each other to prevent the upper buffer solution from leaking.

Embodiment 1 An example of the device of the present invention is shown in FIG.

A neoprene-based rubber (manufactured by Ofuna Seisakusho) packing 2 is attached to the upper buffer tank 1 (freely attachable), and a gel glass plate 4 is pressed against this and adhered by a W clip or the like. In this way, the upper buffer tank can be made thinner than before, and the total capacity of the buffer solution can be reduced. Furthermore, the buffer solution in the upper buffer tank directly touches almost the entire surface of the gel part of the back glass of the gel glass plate, so the cooling effect of the buffer solution makes the temperature of the glass plate uniform. Not only can this be eliminated, but the glass plate can be prevented from cracking due to the non-uniform heat distribution during electrophoresis.

In FIG. 1, the upper buffer tank 1 is detachably attached to the groove 5 of the lower buffer tank 3.

Embodiment 2 Another example of the device of the present invention is shown in FIG.

A hole 7 is formed on the plate surface of the component material of the upper buffer tank 1 shown in FIG.
Is open. This hole is provided on the upper part of the hole corresponding to the migration distance. Therefore, two or more holes may be provided depending on the height.

Then, as shown in FIG. 2, the upper buffer solution replenishing tank 6 is detachably attached in correspondence with this hole.

With the above configuration, the buffer solution successively flows from the holes, and the adverse effect of evaporation of the buffer solution during long-term migration can be eliminated.

[Effect of device]

As described above, according to the device of the present invention, (1) it is possible to make the temperature of the gel glass plate uniform and prevent the development of the smile effect, and (2) it is possible to reduce the volume of the buffer solution. (3) Even with the cooling type, it is easier to assemble than the conventional device and the manufacturing cost can be reduced. (4) If gel glass plates of two or more sizes are prepared, migration is performed only by exchanging the glass plates. A remarkable effect such as that the distance can be adjusted is exhibited.

[Brief description of drawings]

FIG. 1 is a perspective view of a portion related to a buffer tank of one example of the device of the present invention, and FIG. 2 is a perspective view of a portion related to an upper buffer solution replenishing tank of another example of the device of the present invention. 1: Upper buffer tank, 3: Lower buffer tank, 4: Gel glass plate, 6: Upper buffer solution supply tank

Claims (3)

[Scope of utility model registration request] 1. An electrophoresis apparatus including an upper buffer tank, a lower buffer tank and a gel glass plate, wherein the upper buffer tank has a vertically long shape from directly above the lower buffer tank to the upper part of the apparatus, and the gel glass The back glass of the plate constitutes the front part of the upper buffer tank, the gel glass plate is adhered to form the upper buffer tank, and the buffer solution is the gel part of the back glass of the gel glass plate. A cooling type electrophoretic device having a structure capable of directly touching a portion corresponding to substantially the entire surface and constantly communicating with the upper part of the gel in the gel glass plate. 2. A cooling type electrophoresis apparatus according to claim 1, wherein the gel glass plate is provided with two or more kinds in terms of size. 3. The upper buffer tank is provided on a plate surface of its constituent material.
The cooling type electrophoresis apparatus according to claim 1 or 2, wherein the cooling type electrophoresis apparatus has one or more holes capable of stoppering, and has a shape in which a buffer solution replenishing tank is attached corresponding to the holes. .