JP5206611B2 - Gel cassette for electrophoresis - Google Patents

Gel cassette for electrophoresis Download PDF

Info

Publication number
JP5206611B2
JP5206611B2 JP2009168363A JP2009168363A JP5206611B2 JP 5206611 B2 JP5206611 B2 JP 5206611B2 JP 2009168363 A JP2009168363 A JP 2009168363A JP 2009168363 A JP2009168363 A JP 2009168363A JP 5206611 B2 JP5206611 B2 JP 5206611B2
Authority
JP
Japan
Prior art keywords
gel
plate member
electrophoresis
cassette
space
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2009168363A
Other languages
Japanese (ja)
Other versions
JP2011022047A (en
Inventor
達也 増子
覚誠 阿久津
幸司 坂入
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toppan Inc
Original Assignee
Toppan Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toppan Inc filed Critical Toppan Inc
Priority to JP2009168363A priority Critical patent/JP5206611B2/en
Publication of JP2011022047A publication Critical patent/JP2011022047A/en
Application granted granted Critical
Publication of JP5206611B2 publication Critical patent/JP5206611B2/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Landscapes

  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Description

本発明は、電気泳動用ゲル形成のためのカセットに関し、さらに詳しくはタンパク質分離のため分離媒質を簡便に取り出すことが可能な容器、すなわち電気泳動用ゲルカセットに関する。   The present invention relates to a cassette for forming an electrophoresis gel, and more particularly to a container in which a separation medium can be easily taken out for protein separation, that is, an electrophoresis gel cassette.

ヒトゲノムプロジェクトが終了した現在では、タンパク質のプロファイリングの研究が盛んに行われている。またそのような研究の分野だけでなく医療などの分野からも、患者のタンパク質の分析から知見を得ることによる、より適切な診断技術が要求されている。そのためタンパク質を分離するための低コスト・効率的な手法の開発が待たれている。   At the end of the Human Genome Project, protein profiling research is actively underway. Further, not only in the field of such research but also in the field of medical care, there is a demand for more appropriate diagnostic techniques by obtaining knowledge from analysis of patient proteins. Therefore, development of a low cost and efficient method for separating proteins is awaited.

タンパク質を分離するための方法として現在最も有力であるのが二次元電気泳動による分離である。これは個々のタンパク質が持っている電荷、分子量という二つの性質による分離方法を組み合わせたものである。
タンパク質を分子量により分離する方法として最も多く用いられているものはＳＤＳ−ＰＡＧＥ(Sodium Dodecyl Sulfate - Poly Acrylamide Gel Electrophoresis)と呼ばれ、架橋構造をもったアクリルアミドポリマーによる水性ゲル中に、SDS(ドデシル硫酸ナトリウム)処理により電荷を帯びたタンパク質を導入し、電気泳動させるものである。この際ポリアクリルアミドが架橋構造を有することから分子ふるいとしての効果を持ち、タンパク質は分子量ごと分離される。 The most promising method for separating proteins is separation by two-dimensional electrophoresis. This is a combination of separation methods based on the two properties of charge and molecular weight of individual proteins.
The most widely used method for separating proteins by molecular weight is called SDS-PAGE (Sodium Dodecyl Sulfate-Poly Acrylamide Gel Electrophoresis), and SDS (dodecyl sulfate) in an aqueous gel with an acrylamide polymer having a crosslinked structure. Sodium), a charged protein is introduced by treatment and electrophoresed. At this time, since polyacrylamide has a crosslinked structure, it has an effect as a molecular sieve, and proteins are separated according to molecular weight.

ＳＤＳ−ＰＡＧＥにおける分離媒体の構成は、低濃度ポリアクリルアミド水性ゲルからなる濃縮ゲルと、高濃度ポリアクリルアミド水性ゲルからなる分離ゲルからなる。この二つのゲルが接触しており、タンパク質は最初に濃縮ゲルに導入され、濃縮ゲルを通った後に分離ゲルに導入される。
この時用いられるポリアクリルアミド水性ゲルは、アクリルアミドモノマーとＮ,Ｎ'-メチレンビスアクリルアミドのような架橋剤、トリスヒドロキシメチルアミノエタン−ＨＣｌなどの緩衝溶液、水の混合物に過硫酸アンモニウム、Ｎ，Ｎ，Ｎ’，Ｎ’−テトラメチルエチレンジアミンのようなラジカル重合開始剤を導入して得られるゲル前駆溶液を、ゲル形成容器に充填し重合させることにより作製される。
ゲルを内包する容器(以下カセットと呼ぶ)は、ゲル形成領域として直方体である構造を取るものが一般的である。たとえば特許文献１に示されたカセットは、二枚の板の間にゲルを形成する形となっており、この二枚の板を分割することで内包されたゲルを取り出すことができる。 The structure of the separation medium in SDS-PAGE is composed of a concentrated gel composed of a low-concentration polyacrylamide aqueous gel and a separation gel composed of a high-concentration polyacrylamide aqueous gel. The two gels are in contact and the protein is first introduced into the concentrated gel and then through the concentrated gel and then into the separation gel.
The polyacrylamide aqueous gel used at this time includes an acrylamide monomer and a crosslinking agent such as N, N′-methylenebisacrylamide, a buffer solution such as trishydroxymethylaminoethane-HCl, a mixture of water, ammonium persulfate, N, N, It is prepared by filling a gel-forming container with a gel precursor solution obtained by introducing a radical polymerization initiator such as N ′, N′-tetramethylethylenediamine and polymerizing it.
A container containing a gel (hereinafter referred to as a cassette) generally has a rectangular parallelepiped structure as a gel formation region. For example, the cassette shown in Patent Document 1 has a shape in which a gel is formed between two plates, and the contained gel can be taken out by dividing the two plates.

このようなＰＡＧＥに用いるゲルの作製は煩雑であるため、現在ではあらかじめカセットの内部にゲルが形成されているプレキャストゲルも市販されている。そのようなプレキャストゲルカセットを用いれば、試験者はゲル作製を行う必要がなく、電気泳動を行った後、ゲル中に導入されたタンパク質の位置を可視化する作業を行うためにカセットからゲルと取り出すことになる。   Since preparation of such a gel for PAGE is complicated, a precast gel in which a gel is formed in advance in the cassette is also commercially available. If such a precast gel cassette is used, the tester does not need to prepare a gel, and after performing electrophoresis, the tester takes out the gel from the cassette in order to visualize the position of the protein introduced into the gel. It will be.

特開２００２−２２８６３２号公報JP 2002-228632 A

しかしながら、ゲルの取り出し作業において、ゲルの強度が高くないことから取出しには熟練した者が慎重な作業をする必要があり、タンパク質分離のひとつの問題となっている。たとえば特許文献１に示されたカセットと同様の構造を持つカセットにゲルを形成しゲルの取り出しを行う時、開封時に二枚の面のどちらにゲルが残るかは不確定であり、さらにはゲルが両方の面に分離してしまうなどの問題があった。   However, since the strength of the gel is not high in the gel removal operation, it is necessary for a skilled person to carefully perform the removal, which is one problem of protein separation. For example, when a gel is formed on a cassette having the same structure as the cassette disclosed in Patent Document 1 and the gel is taken out, it is uncertain which gel remains on the two surfaces when opened, and further the gel However, there were problems such as separation on both sides.

本発明は、上述のような従来の問題を解決したものであり、その目的は、カセットからゲルを取り出す工程において、ゲルを簡便に取り出すことが可能で、かつ取り出しの作業によりゲルが破損することがない電気泳動用ゲルカセットを提供することにある。   The present invention solves the conventional problems as described above, and its purpose is that the gel can be easily taken out in the step of taking out the gel from the cassette, and the gel is damaged by the removing operation. It is an object of the present invention to provide a gel cassette for electrophoresis that does not have any.

上記の目的を達成するために請求項１の発明は、電気泳動用ゲル生成用のカセットであって、非導電性を有する第１板部材と、前記第１板部材の一方の面に接合された非導電性を有する第２板部材と、前記第１板部材と前記第２板部材との間に形成されたゲル収容空間と、前記第１及び第２板部材の端部に形成され前記ゲル収容空間にゲル前駆溶液を充填するゲル充填口と、前記第１板部材の前記ゲル収容空間と反対の面に設けられ、前記ゲル収容空間へのエアの導入を可能とし着脱可能な封止部材で封止された通気孔と、前記第２板部材の前記ゲル収容空間と反対の面に設けられ、前記ゲル収容空間への水の注入を可能とし着脱可能な封止部材で封止された通水孔とを備えることを特徴とする。   In order to achieve the above object, an invention according to claim 1 is a cassette for generating an electrophoresis gel, which is joined to a first plate member having non-conductivity and one surface of the first plate member. A non-conductive second plate member, a gel containing space formed between the first plate member and the second plate member, and formed at an end of the first and second plate members. A gel filling port for filling the gel containing space with the gel precursor solution and a surface of the first plate member opposite to the gel containing space, which allows air to be introduced into the gel containing space and is removable. A vent hole sealed with a member and a surface opposite to the gel accommodating space of the second plate member are provided and sealed with a removable sealing member that allows water to be injected into the gel accommodating space. And a water passage hole.

請求項２の発明は、請求項１記載の電気泳動用ゲルカセットにおいて、前記ゲル収容空間は直方体を呈することを特徴とする。
請求項３の発明は、請求項１または２記載の電気泳動用ゲルカセットにおいて、前記ゲル充填口と反対の側で前記第１及び第２板部材の一方の箇所に前記ゲル収容空間に連通するバッファー接触口が設けられていることを特徴とする。 According to a second aspect of the present invention, in the gel cassette for electrophoresis according to the first aspect, the gel accommodating space has a rectangular parallelepiped shape.
According to a third aspect of the present invention, in the gel cassette for electrophoresis according to the first or second aspect, the gel containing space communicates with one of the first and second plate members on the side opposite to the gel filling port. A buffer contact port is provided.

請求項４の発明は、請求項１乃至３に何れか１項記載の電気泳動用ゲルカセットにおいて、前記第１板部材の前記ゲル収容空間と反対の面に凹部が形成され、前記通気孔は、前記凹部の底部に形成されていることを特徴とする。   According to a fourth aspect of the present invention, in the gel cassette for electrophoresis according to any one of the first to third aspects, a recess is formed on a surface of the first plate member opposite to the gel accommodating space, It is formed in the bottom part of the said recessed part, It is characterized by the above-mentioned.

請求項５の発明は、請求項１乃至４に何れか１項記載の電気泳動用ゲルカセット前記第２板部材の前記ゲル収容空間と反対の面に凹部が形成され、前記通水孔は、前記凹部の底部に形成されていることを特徴とする。   A fifth aspect of the present invention is the gel cassette for electrophoresis according to any one of the first to fourth aspects, wherein a concave portion is formed on a surface opposite to the gel-accommodating space of the second plate member, It is formed in the bottom part of the said recessed part, It is characterized by the above-mentioned.

請求項６の発明は、請求項１乃至５に何れか１項記載の電気泳動用ゲルカセットにおいて、前記封止部材は、栓体または接着シールの何れかであることを特徴とする。   A sixth aspect of the present invention is the gel cassette for electrophoresis according to any one of the first to fifth aspects, wherein the sealing member is either a plug or an adhesive seal.

請求項７の発明は、請求項１、２、３、５、６に何れか１項記載の電気泳動用ゲルカセットにおいて、前記第１板部材の前記ゲル収容空間と反対の面に薄肉部が形成され、前記通気孔は前記薄肉部に形成されていることを特徴とする。   The seventh aspect of the invention is the electrophoresis gel cassette according to any one of the first, second, third, fifth, and sixth aspects, wherein the thin plate portion is provided on a surface of the first plate member opposite to the gel containing space. The vent hole is formed in the thin portion.

本発明の電気泳動用ゲルカセットにおいては、ゲル前駆溶液をゲル収容空間に充填して電気泳動を行った後に、通気孔の封止部材を除去することで該通気孔を通して導入されるエアによりゲル収容空間の第１板部材側内面からゲルを分離し、さらに、通水孔の封止部材を除去することで該通水孔を通して注入される水によりゲル収容空間の第２板部材側内面からゲルを分離できる。これにより、カセット分割時にゲルがカセット内の決まった場所に残り、またゲルを傷つけることなく目的の場所に配置することができるほか、ゲルを簡便にかつ破損することなく取り出すことができる。   In the gel cassette for electrophoresis of the present invention, the gel precursor solution is filled in the gel containing space and subjected to electrophoresis, and then the air is introduced through the air hole by removing the sealing member of the air hole. The gel is separated from the inner surface on the first plate member side of the housing space, and further, the sealing member of the water passage hole is removed to remove water from the second plate member side inner surface of the gel housing space by water injected through the water passage hole. The gel can be separated. As a result, the gel remains in a predetermined location in the cassette when the cassette is divided, and the gel can be placed at a target location without damaging the gel, and the gel can be taken out easily and without being damaged.

本発明にかかる電気泳動用ゲルカセットの全体の構成を示す斜視図である。It is a perspective view which shows the whole structure of the gel cassette for electrophoresis concerning this invention. 本発明にかかる電気泳動用ゲルカセットを前方から見たときの分解斜視図である。It is a disassembled perspective view when the gel cassette for electrophoresis concerning this invention is seen from the front. 本発明にかかる電気泳動用ゲルカセットを後方から見たときの分解斜視図である。It is a disassembled perspective view when the gel cassette for electrophoresis concerning this invention is seen from back. （Ａ）〜（Ｃ）は本発明にかかる電気泳動用ゲルカセットの通気孔の作成工程を示す説明図である。(A)-(C) are explanatory drawings which show the preparation process of the vent hole of the gel cassette for electrophoresis concerning this invention.

以下、本発明の実施の態様について、図面を参照しながら詳細に説明する。
図１〜図３において、気泳動用ゲル生成用のカセット１０は、非導電性を有する第１板部材１と、この第１板部材１の一方の面に接合された非導電性を有する第２板部材２を備える。この第１板部材１と第２板部材２は、超音波溶着や接着剤の介在等の適切な手法で張り合わされる。
第１板部材１と第２板部材２との間には、第２板部材２の内面を直方体状に切り欠くことでゲル収容空間３が形成されている。
また、第１板部材１および第２板部材の端部には、第１板部材１の端部をゲル収容空間３の幅寸法に合わせて凹状に切り欠くことで、ゲル収容空間３にゲル前駆溶液を充填するゲル充填口５が形成されている。さらに、ゲル充填口５と反対の側で第１板部材１の箇所には、ゲル収容空間５に連通するスリット状のバッファー接触口４がゲル収容空間５の幅方向に延在して設けられている。 Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings.
In FIG. 1 to FIG. 3, the electrophoresis gel generating cassette 10 includes a first plate member 1 having non-conductivity and a non-conductive first plate member 1 joined to one surface of the first plate member 1. A two-plate member 2 is provided. The first plate member 1 and the second plate member 2 are bonded to each other by an appropriate method such as ultrasonic welding or adhesive intervention.
Between the 1st board member 1 and the 2nd board member 2, the gel accommodation space 3 is formed by notching the inner surface of the 2nd board member 2 in a rectangular parallelepiped shape.
Further, at the end portions of the first plate member 1 and the second plate member, the end portion of the first plate member 1 is notched in a concave shape in accordance with the width dimension of the gel storage space 3, so that the gel storage space 3 has a gel. A gel filling port 5 for filling the precursor solution is formed. Furthermore, a slit-like buffer contact port 4 communicating with the gel containing space 5 is provided in the first plate member 1 on the side opposite to the gel filling port 5 so as to extend in the width direction of the gel containing space 5. ing.

第１板部材１のゲル収容空間３と反対の面には、図１及ぶ図２に示すように、ゲル収容空間３へのエアの導入を可能とし、かつ着脱可能な封止部材６で封止された通気孔７が設けられている。また、第２板部材２のゲル収容空間３と反対の面には、ゲル収容空間３への水の注入を可能とし、かつ着脱可能な封止部材８で封止された通水孔９が設けられている。   As shown in FIG. 1 and FIG. 2, the surface of the first plate member 1 opposite to the gel accommodating space 3 can be introduced with air and sealed with a removable sealing member 6. A stopped vent 7 is provided. Further, on the surface opposite to the gel accommodation space 3 of the second plate member 2, there is a water passage hole 9 that allows water to be injected into the gel accommodation space 3 and is sealed with a removable sealing member 8. Is provided.

次に、第１板部材１に封止部材６で封止された通気孔７を形成する場合について、図４を参照して説明する。
まず、図４（Ａ）に示すように、第１板部材１のゲル収容空間３と反対の面に、第１板部材１の厚さより薄くした凹部１ａを形成し、この凹部１ａの底部に通気孔７を形成するための仮孔１ｂを設け、この仮孔１ｂに封止部材６を構成する熱可塑性樹脂６ａを嵌め込む。その後、図４（Ｂ）に示すように、熱可塑性樹脂６ａが溶解するのに十分な温度に加熱した封止用の栓体６ｂを熱可塑性樹脂６ａに差し込む。その後、熱可塑性樹脂６ａが冷却されれば、封止部材６で封止された通気孔７が構成される。この場合、封止用の栓体６ｂは熱可塑性樹脂６ａよりも硬度が強いとが好ましい。また、図４（Ｃ）に示すように、栓体６ｂを除去することで通気孔７が開放され、ゲル収容空間３へのエアの導入が可能になる。
なお、凹部１ａを設ける理由は、封止用の栓体６ｂが他の物体を干渉して破損される防止するため、または通気孔７の形成を容易にするためである。
また、第２板部材２の封止部材８及びこれを封止する通水孔９の構成は、上記第１板部材１の封止部材６及び通気孔７と同様な構成になっている。 Next, the case where the air hole 7 sealed with the sealing member 6 is formed in the first plate member 1 will be described with reference to FIG.
First, as shown in FIG. 4 (A), a concave portion 1a is formed on the surface of the first plate member 1 opposite to the gel accommodating space 3 and is made thinner than the thickness of the first plate member 1, and at the bottom of the concave portion 1a. A temporary hole 1b for forming the vent hole 7 is provided, and a thermoplastic resin 6a constituting the sealing member 6 is fitted into the temporary hole 1b. After that, as shown in FIG. 4B, the sealing plug 6b heated to a temperature sufficient to dissolve the thermoplastic resin 6a is inserted into the thermoplastic resin 6a. Then, if the thermoplastic resin 6a is cooled, the vent hole 7 sealed with the sealing member 6 is formed. In this case, it is preferable that the sealing plug 6b has a higher hardness than the thermoplastic resin 6a. Further, as shown in FIG. 4C, by removing the plug body 6b, the vent hole 7 is opened, and air can be introduced into the gel containing space 3.
The reason for providing the recess 1a is to prevent the sealing plug 6b from being damaged by interference with other objects, or to facilitate the formation of the vent hole 7.
Moreover, the structure of the sealing member 8 of the 2nd board member 2 and the water flow hole 9 which seals this is the same structure as the sealing member 6 and the vent hole 7 of the said 1st board member 1. FIG.

次に、上記構成のカセットを用いた電気泳動について説明する。
ゲル前駆溶液の充填はバッファー接触口４を公知のシール材料で封止した状態で、ゲル充填口５から充填される。ゲル形成後にバッファー接触口４のシール材を取り外し公知の方法で電気泳動を行う。
電気泳動を行った後、封止用の栓体６ｂを図４（Ｃ）に示すように取り外し、通気孔７を形成する。そして通気孔７を通してエアをゲル収容空間３に導入することにより、ゲル収容空間３の第１板部材１側内面とゲルとの間に隙間が発生し、この隙間により第１板部材１側内面からゲルを分離する。これに伴い、第２板部材２を下にして、第１板部材１を第２板部材２から分離することが可能になる。その後、封止部材８を取り外し、通水孔９を形成する。これにより、通水孔９から水を注入することでゲル収容空間３の第２板部材２側内面とゲルとの間に隙間が発生し、この隙間により第２板部材２側内面からゲルを分離し、ゲルを取り出すことが可能になる。この場合、第２板部材２のゲル接触面３ａ（図３参照）にのみ親水表処理を施しても良い。 Next, electrophoresis using the cassette having the above configuration will be described.
The gel precursor solution is filled from the gel filling port 5 with the buffer contact port 4 sealed with a known sealing material. After the gel is formed, the sealing material of the buffer contact port 4 is removed and electrophoresis is performed by a known method.
After the electrophoresis, the sealing plug 6b is removed as shown in FIG. And by introducing air into the gel accommodation space 3 through the vent hole 7, a gap is generated between the inner surface of the gel accommodation space 3 on the first plate member 1 side and the gel, and this clearance causes the first plate member 1 side inner surface. Separate the gel from. Accordingly, the first plate member 1 can be separated from the second plate member 2 with the second plate member 2 facing down. Then, the sealing member 8 is removed and the water passage hole 9 is formed. Thereby, by injecting water from the water passage hole 9, a gap is generated between the inner surface of the gel housing space 3 on the second plate member 2 side and the gel, and the gel is introduced from the inner surface of the second plate member 2 side by this gap. It is possible to separate and remove the gel. In this case, hydrophilic surface treatment may be performed only on the gel contact surface 3a (see FIG. 3) of the second plate member 2.

次に、本発明の実施例について説明する。
ゲル形成用のゲル収容空間３の厚みが１ｍｍであり、ゲル収容空間３の容量が５ｍｌである図１に示した構造を有するカセットに、電気泳動用ポリアクリルアミドゲルを形成し、電気泳動を行った後、封止用の栓体６ｂを折り、通気孔７を開口してエアをゲル収容空間３に導入することで第１板部材１側内面とゲルとの間に間隙を形成した。
その後、第２板部材２を下にして、第１板部材１を第２板部材２から分離した。この操作を行うと第１板部材１と第２板部材２の分割時において、ゲルは第２板部材２側に残り、ゲルの一部が第１板部材１側に吸着したまま分割されてしまうようなことも起こらなかった。 Next, examples of the present invention will be described.
A polyacrylamide gel for electrophoresis is formed on a cassette having the structure shown in FIG. 1 in which the gel containing space 3 for gel formation has a thickness of 1 mm and the capacity of the gel containing space 3 is 5 ml, and electrophoresis is performed. After that, the sealing plug 6b was folded, the vent hole 7 was opened, and air was introduced into the gel containing space 3, thereby forming a gap between the inner surface of the first plate member 1 side and the gel.
Thereafter, the first plate member 1 was separated from the second plate member 2 with the second plate member 2 facing down. When this operation is performed, when the first plate member 1 and the second plate member 2 are divided, the gel remains on the second plate member 2 side, and a part of the gel is divided while being adsorbed on the first plate member 1 side. Nothing happened.

染色液で満たされた容器の上で、封止部材８を取り外すことで形成された通水孔９から、水を注入するとゲルと第２板部材２側内面との間に水が入り込み、ゲル充填口５を下に傾けるとゲルがゲル充填口５から形を崩すことなく滑り落ちた。染色液で満たされた容器の上でこの操作を行えば、ゲルに直接、または何かの器具を用いることなくゲルを染色液中に落とすことができた。   When water is injected from the water passage hole 9 formed by removing the sealing member 8 on the container filled with the staining solution, water enters between the gel and the second plate member 2 side inner surface, and the gel When the filling port 5 was tilted downward, the gel slipped from the gel filling port 5 without breaking its shape. If this operation was performed on a container filled with the staining solution, the gel could be dropped into the staining solution directly or without using any instrument.

以上、本発明の実施形態について図面を参照して詳述したが、具体的な構成はこの実施形態に限られるものではなく、本発明の要旨を逸脱しない範囲の設計変更等も含まれる。また、本発明ではゲルの充填から説明を行っているが、プレキャスト用のカセットとしても適応可能である。
また、本発明における第１板部材１及び第２板部材２の封止部材及びこれで封止される通気孔または通水孔は、電気泳動時はふさがれていて、カセット分離時には外気を導入できる構造であればよいので、上記実施の形態に示す構造のものに限定されない。例えば、予め通気孔または通水孔を開けておき、これを粘着テープや粘着シール等で塞いで密閉してゲル充填及び分析を行い、しかるべきときに粘着テープや粘着シールをはがして外気または水を導入したりしてもよい。また、第１板部材１及び第２板部材２の外表面に、他の箇所より薄く壊れやすい部分を予め設けておき、必要に応じてピンなどでつついて穴を開け、外気または水を導入する方法が考えられる。
さらに、通気孔または通水孔は複数あっても良く、例えば、ゲルの状態に応じて分析に影響の出ないところを選択して（破壊して）外気または水を導入するようにしてもよい。 As mentioned above, although embodiment of this invention was explained in full detail with reference to drawings, the concrete structure is not restricted to this embodiment, The design change etc. of the range which does not deviate from the summary of this invention are included. Further, in the present invention, the description is given from the filling of the gel, but it can also be applied as a precast cassette.
Further, the sealing member of the first plate member 1 and the second plate member 2 and the vent hole or water passage hole sealed by the first plate member 1 and the second plate member 2 in the present invention are blocked during electrophoresis, and external air is introduced during cassette separation. Any structure can be used, and the structure is not limited to the structure described in the above embodiment. For example, vent holes or water passage holes are opened in advance and sealed with an adhesive tape or adhesive seal to perform gel filling and analysis. When appropriate, the adhesive tape or adhesive seal is removed to remove outside air or water. Or may be introduced. In addition, on the outer surface of the first plate member 1 and the second plate member 2, a portion that is thinner and more fragile than other portions is provided in advance, and a hole is made with a pin or the like as necessary to introduce outside air or water. A way to do this is conceivable.
Further, there may be a plurality of air holes or water holes. For example, the outside air or water may be introduced by selecting (destroying) a place that does not affect the analysis according to the state of the gel. .

１…第１板部材、２…第２板部材、３…ゲル収容空間、４…バッファー接触口、５…ゲル充填口、６…封止部材、７…通気孔、８…封止部材、９…通水孔、１０…カセット、１ａ…凹部、１ｂ…仮孔、６ａ…熱可塑性樹脂、６ｂ…栓体。   DESCRIPTION OF SYMBOLS 1 ... 1st board member, 2 ... 2nd board member, 3 ... Gel accommodation space, 4 ... Buffer contact port, 5 ... Gel filling port, 6 ... Sealing member, 7 ... Air vent, 8 ... Sealing member, 9 ... water passage hole, 10 ... cassette, 1a ... recess, 1b ... temporary hole, 6a ... thermoplastic resin, 6b ... plug.

Claims (7)

電気泳動用ゲル生成用のカセットであって、
非導電性を有する第１板部材と、
前記第１板部材の一方の面に接合された非導電性を有する第２板部材と、
前記第１板部材と前記第２板部材との間に形成されたゲル収容空間と、
前記第１及び第２板部材の端部に形成され前記ゲル収容空間にゲル前駆溶液を充填するゲル充填口と、
前記第１板部材の前記ゲル収容空間と反対の面に設けられ、前記ゲル収容空間へのエアの供給を可能とし着脱可能な封止部材で封止された通気孔と、
前記第２板部材の前記ゲル収容空間と反対の面に設けられ、前記ゲル収容空間への水の供給を可能とし着脱可能な封止部材で封止された通水孔とを備える、
ことを特徴とする電気泳動用ゲルカセット。 A cassette for gel generation for electrophoresis,
A first plate member having non-conductivity;
A non-conductive second plate member joined to one surface of the first plate member;
A gel containing space formed between the first plate member and the second plate member;
A gel filling port that is formed at an end of the first and second plate members and fills the gel containing space with a gel precursor solution;
A vent hole provided on a surface opposite to the gel accommodating space of the first plate member, and capable of supplying air to the gel accommodating space and sealed with a removable sealing member;
Provided on a surface opposite to the gel housing space of the second plate member, and provided with a water passage hole sealed with a removable sealing member that enables water supply to the gel housing space;
A gel cassette for electrophoresis. 前記ゲル収容空間は直方体を呈することを特徴とする請求項１記載の電気泳動用ゲルカセット。   The gel cassette for electrophoresis according to claim 1, wherein the gel accommodating space has a rectangular parallelepiped shape. 前記ゲル充填口と反対の側で前記第１及び第２板部材の一方の箇所に前記ゲル収容空間に連通するバッファー接触口が設けられていることを特徴とする請求項１または２記載の電気泳動用ゲルカセット。   3. The electricity according to claim 1, wherein a buffer contact port communicating with the gel containing space is provided at one location of the first and second plate members on the side opposite to the gel filling port. Gel cassette for electrophoresis. 前記第１板部材の前記ゲル収容空間と反対の面に凹部が形成され、前記通気孔は、前記凹部の底部に形成されていることを特徴とする請求項１乃至３に何れか１項記載の電気泳動用ゲルカセット。   The concave portion is formed on the surface of the first plate member opposite to the gel accommodating space, and the vent hole is formed at the bottom of the concave portion. Gel cassette for electrophoresis. 前記第２板部材の前記ゲル収容空間と反対の面に凹部が形成され、前記通水孔は、前記凹部の底部に形成されていることを特徴とする請求項１乃至４に何れか１項記載の電気泳動用ゲルカセット。   The concave part is formed in the surface opposite to the gel accommodating space of the second plate member, and the water passage hole is formed in the bottom part of the concave part. The gel cassette for electrophoresis as described. 前記封止部材は、栓体または接着シールの何れかであることを特徴とする請求項１乃至５に何れか１項記載の電気泳動用ゲルカセット。   The gel cassette for electrophoresis according to any one of claims 1 to 5, wherein the sealing member is either a plug or an adhesive seal. 前記第１板部材の前記ゲル収容空間と反対の面に薄肉部が形成され、前記通気孔は前記薄肉部に形成されていることを特徴とする請求項１、２、３、５、６に何れか１項記載の電気泳動用ゲルカセット。   The thin plate portion is formed on a surface of the first plate member opposite to the gel accommodating space, and the vent hole is formed in the thin plate portion. The gel cassette for electrophoresis according to any one of claims.
JP2009168363A 2009-07-17 2009-07-17 Gel cassette for electrophoresis Expired - Fee Related JP5206611B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2009168363A JP5206611B2 (en) 2009-07-17 2009-07-17 Gel cassette for electrophoresis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2009168363A JP5206611B2 (en) 2009-07-17 2009-07-17 Gel cassette for electrophoresis

Publications (2)

Publication Number Publication Date
JP2011022047A JP2011022047A (en) 2011-02-03
JP5206611B2 true JP5206611B2 (en) 2013-06-12

Family

ID=43632255

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2009168363A Expired - Fee Related JP5206611B2 (en) 2009-07-17 2009-07-17 Gel cassette for electrophoresis

Country Status (1)

Country Link
JP (1) JP5206611B2 (en)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0330853Y2 (en) * 1985-05-20 1991-06-28
JPH0634684Y2 (en) * 1985-06-03 1994-09-07 寶酒造株式会社 Cooled electrophoresis device
US6162342A (en) * 1999-02-12 2000-12-19 Bio-Rad Laboratories, Inc. Rapid assembly casting system for slab gels
US6521111B1 (en) * 2000-04-10 2003-02-18 Invitrogen Corporation Methods and articles for labeling polymer gels
JP2007259774A (en) * 2006-03-29 2007-10-11 Morinaga Milk Ind Co Ltd Confectionery
EP2180316A4 (en) * 2007-08-17 2010-10-20 Hymo Corp Precast gel for electrophoresis, method for production thereof, and use thereof

Also Published As

Publication number Publication date
JP2011022047A (en) 2011-02-03

Similar Documents

Publication Publication Date Title
JP6734482B2 (en) Pouch exterior material for secondary battery, pouch type secondary battery using the same, and manufacturing method thereof
US20200377246A1 (en) Systems and methods for multi-stage sealing of contact lens packaging
JP7045606B2 (en) Sealing block for pouch-type secondary battery to prevent cracks, pouch-type battery case manufactured using this, and sealing method for pouch-type battery case
BRPI0517029B1 (en) "BATTERY SET"
US20060099496A1 (en) Separator container
JP6404045B2 (en) Lid, electric element, electric element unit, and method of manufacturing lid
JP2008264561A5 (en)
JP5206611B2 (en) Gel cassette for electrophoresis
CN104067403A (en) Pouch-type battery, and apparatus and method for sealing pouch-type battery
CN104733653A (en) Lead-acid battery, manufacturing method of the same, and lid member for lead-acid battery
Bitbol et al. Dynamical membrane curvature instability controlled by intermonolayer friction
KR101817375B1 (en) Pouch for rechargeable battery and rechargeable battery using the same
JP2005530151A (en) Method and apparatus for low resistance electrophoresis of precast hydratable separation media
US20030127331A1 (en) Two-dimensional electrophoresis method and cassette
JP5108146B2 (en) Welded electrophoresis cassette without leaking weld line
JP5353619B2 (en) Method for manufacturing electrophoresis medium cassette
JP5246128B2 (en) Method for producing gel cassette for electrophoresis and filling method for gel for electrophoresis
JP5307077B2 (en) Electrophoresis gel cassette and method for producing precast gel cassette
JP2011022061A (en) Cassette sealing material for electrophoresis
JPWO2016035752A1 (en) Reference electrode element and ion sensor device
US10737409B2 (en) Apparatus and method of controlling stiffness of soft material
JP5721390B2 (en) Two-dimensional electrophoresis cassette
JP2005062075A (en) Microgel cassette for electrophoresis
JP5287569B2 (en) Method for producing gel cassette for electrophoresis
CN210658315U (en) Novel steel sheet pile structure suitable for underwater operation

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20120621

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20130115

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20130122

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20130204

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20160301

Year of fee payment: 3

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

LAPS Cancellation because of no payment of annual fees