JPH0634676Y2 - Test solution spectroscopic analyzer - Google Patents

Description

[Detailed Description of the Invention] "Industrial field of application" The present invention relates to an apparatus for measuring the functions and properties of cells, in particular, by detecting the absorbance or fluorescence of a test solution, the enzyme-labeled antigen-body reaction is fluorescent. The present invention relates to a reagent solution spectroscopic analyzer suitable for immunoassay by a labeling method or the like.

"Prior Art" A conventional sample liquid spectroscopic analyzer of this type is disclosed in JP-A-60-39533.
Things like issues are known. As shown in FIG. 2, this sample liquid spectroscopic analyzer introduces light from a light source 1 into a wavelength selection device 4 via a condenser lens 2 and a slit 3. The wavelength selection device 4 includes a chopper device for rotating the slit plate 5 with a motor 6, a diffraction grating or an interference filter 7, and a condenser lens 8. The diffraction grating or the interference filter 7 uses the optical fiber 9 to extract only the light of a predetermined wavelength out of the light emitted from the light source 1.
The reagent solution 12 in the storage recess 11 of the microplate 10 is irradiated via the. The light passing through the reagent solution 12 is received by the light receiver 13 and sent to the measuring device 14, and the measuring device 14 compares the reference light from the optical fiber 9 with the detection signal of the detector 15 which receives the reference light. The absorbance or fluorescence of is measured.

However, since this reagent solution spectroscopic analyzer measures light by irradiating each reagent solution 13 from the optical fiber at the same time,
The light to be measured which has passed through each reagent solution 13 radiates while being spread due to scattering in the reagent solution 13, and each element a to
In n, not only the light to be measured that has passed through the corresponding reagent solution 13 but also the light to be measured that has passed through the adjacent reagent solution 13 is received, as indicated by the overlapping diagonal line 16 in FIG. It was also the cause. Moreover, the optical fiber 9 uses a single optical beam output from the wavelength selecting device 4 as a microplate.
In order to irradiate a large number of 10 test solutions 12, it has to be branched by a branching device or the like, which complicates the structure and leads to a sharp rise in price.

"Problems to be solved by the invention" In view of the above circumstances, the present invention allows each element of the photodetector to receive and measure only the light to be measured that has passed through the corresponding reagent solution, and to measure in adjacent reagent solutions. Provided is a reagent solution spectroscopic analyzer that does not mix measurement light that has passed through and causes a measurement error, and that also distributes light emitted from a wavelength selection device according to each reagent solution of a microplate. The purpose is to

"Means to be Solved by the Invention" The present invention irradiates a light source, a wavelength selecting device for selecting light of a predetermined wavelength from the light from the light source, and light from the wavelength selecting device to a reagent solution of a microplate. The number of reagent solutions to be stored in the microplate in a reagent solution spectroscopic analyzer consisting of an optical fiber and a light receiver that receives the measured light that has passed through the reagent solution of the microplate and measures the absorbance or fluorescence of the reagent solution. A large number of optical fibers corresponding to the above are arranged between the microplate and the wavelength selecting device, and the input ends of the respective optical fibers are arranged in a circle with a constant radius. Between the optical fiber and the input end of the optical fiber, one end of which is opposed to the emission port of the wavelength selection device, and the other end of which is bent to face the input end of each optical fiber, which is arranged circumferentially. It is characterized by a sample solution spectroscopic analysis device in which an optical fiber is provided and the rotating optical fiber is connected to a motor.

[Operation] The present invention rotates the rotating optical fiber with the motor, and allows only the light of a predetermined wavelength out of the radiated light from the light source to enter the rotating optical fiber from the wavelength selection device. Light is sequentially incident on each optical fiber from the rotating optical fiber, and a large number of test solutions of the microplate are sequentially irradiated with light from each optical fiber, and the measuring instrument measures the absorbance or fluorescence by the measured light passing through each test solution.

[Embodiment] An embodiment of the reagent solution spectroscopic analyzer according to the present invention will be described below with reference to the drawings. In FIG. 1, 17 is a light source such as an iodine tungsten lamp. The light emitted from the light source 17 is input to the wavelength selection device 20 via the condenser lens 18 and the slit 19 for narrowing the emission area. The wavelength selection device 20 includes an interference filter 21 and a condenser lens 22. Of course, the interference filter 21 can be used instead of the diffraction grating. Behind the wavelength selection device 20,
A rotating optical fiber 23 is provided. Rotating optical fiber 23
Is provided on a rotary drum 25 rotated by a motor 24. One end of the rotary optical fiber 23 is located at the center of rotation of the rotary drum 25 and is arranged to face the radiation port of the wavelength selection device 20. The rotary optical fiber 23 is bent and the other end thereof is fixedly provided at a position of a constant radius r from the center of rotation of the rotary drum 25. The rotating optical fiber 23,
The number is not necessarily limited to one, and may be two or more. In this case, one end is bundled with the rotation center of the rotary drum 25 and the other end is drawn with the rotation of the rotary drum 25. They are arranged at regular intervals on the circumference of r. A large number of optical fibers 26 are arranged behind the rotary drum 25. The optical fibers 26 are arranged between the rotary drum 25 and the microplate 27 in the number corresponding to the number of the storage recesses 28 formed in the microplate 27. One end of the optical fiber 26 is arranged in a ring shape on the radiation side of the rotary drum 25 and at a position on the circumference of the radius r from the center of rotation. The other end of the optical fiber 26 is provided with a condensing lens 29, and the receiving recesses of the microplate 27 are accommodated.
It is arranged below 28. The microplate 27 is formed of a translucent material, and the storage recesses 28 are also formed.
It stores the test solution 30 to be measured. Above the microplate 27, a light receiver 31 composed of an element corresponding to the reagent solution 30 is arranged. The light receiver 31 is connected to the measuring device 32. One of the optical fibers 26 is taken out separately for reference and is made to be received by the detector 34 via the condenser lens 33. The detector 34 is connected to the measuring device 32.

Then, the reagent solution 30 uses an antigen or an antibody adsorbed to a certain carrier to capture the antibody or the antigen in the solution by an antigen-antibody reaction, and then similarly acts an enzyme-labeled antibody,
After washing to remove the unreacted portion and adding an enzyme substrate to develop color, this is stored in each storage recess 28 of the microplate 27.

The light from the light source 17 is focused through the condenser lens 18 and the slit 19 and then enters the wavelength selection device 20. Wavelength selection device
At 20, at the interference filter 21, only light having a predetermined wavelength is condensed at one end of the rotary optical fiber 23 by the condenser lens 22 and enters. Since the rotating optical fiber 23 is rotated at a constant rotation speed together with the rotating drum 25 by the drive of the motor 24, the light emitted from the other end of the rotating optical fiber 23 is
Light is sequentially incident on one end of 26. Therefore, rotating optical fiber
23 functions not only as a distributor from the wavelength selection device 20 to each optical fiber 26, but also as a chopper device.
From each optical fiber 26, each sample solution 30 in the microplate 27 is sequentially irradiated via a condenser lens 29. The light to be measured that has passed through each reagent solution 30 is received by the light receiver 31 and sent to the measuring device 32. The measuring device 32 compares the peak value of the signal to be measured sequentially sent from each element of the light receiver 31 with the reference signal sent from the optical fiber 26a through the detector 34 to determine the absorbance or fluorescence of each reagent solution 30. Measure the degree. In this case, since the peak value of the measured signal is measured in comparison with the reference signal, even if the measured light leaks from the reagent solution 30 currently being measured to the element of the adjacent light receiver 31, it may be erroneously measured. Absent.

As another embodiment, as shown by the chain double-dashed line in FIG. 1, a rotary encoder 35 for detecting the rotation angle of the rotary drum 25.
Is attached to the motor 24 or the rotating drum 25, and the rotary encoder 35 is connected to the measuring device 32. And
The rotary encoder 35 detects the optical fiber 26 entering from the rotary optical fiber 23, and the measuring instrument 32 finds the element of the photodetector 31 corresponding to the optical fiber 26, whereby only the element of the photodetector 31 is measured. The measuring device 32 takes in the signal and performs a measuring operation. By the above operation, it is possible to prevent a measurement error from occurring even if the measured light leaks and is received in addition to the element of the light receiver 31 to be measured.

“Effect of device” As described above, according to the reagent solution spectroscopic analyzer according to the present invention,
Each element of the photodetector receives and measures only the measured light that has passed through the corresponding reagent solution, and does not cause measurement error due to mixing of the measured light that has passed through the adjacent reagent solution. The configuration in which the light emitted from the selection device is distributed in correspondence with each sample solution of the microplate is extremely simple, and the conventional chomper device can also be used to reduce the number of parts.

[Brief description of drawings]

FIG. 1 is a block diagram showing an embodiment of a sample solution spectroscopic analyzer according to the present invention, and FIG. 2 is a block diagram of a conventional sample solution spectroscopic analyzer. 17 …… Light source, 20 …… Wavelength selector 23 …… Rotating optical fiber, 24 …… Motor 26 …… Optical fiber, 27 …… Microplate 30 …… Test solution, 31 …… Receiver 32 …… Measuring instrument

Claims (1)

[Scope of utility model registration request] 1. A light source, a wavelength selecting device for selecting light of a predetermined wavelength from the light from the light source, and an optical fiber for irradiating the sample solution of the microplate with the light from the wavelength selecting device.
The photoreceiver receives the light to be measured that has passed through the reagent solution of the microplate, and consists of a measuring instrument that measures the absorbance or fluorescence of the reagent solution, and has a large number of optical fibers corresponding to the number of reagent solutions to be stored in the microplate. In a sample solution spectroscopic analyzer arranged between the microplate and the wavelength selecting device, the input ends of the respective optical fibers are arranged in a circle having a constant radius, and the wavelength selecting device and the input ends of the respective optical fibers are arranged. A rotary optical fiber having one end opposed to the radiation port of the wavelength selection device and the other end bent to be opposed to the circumferentially arranged input end of each optical fiber is disposed between the rotary optical fiber and the rotary optical fiber. Is connected to a motor, one of the optical fibers is taken out for reference, and a reference signal, which is a signal detected by the optical fiber for reference, is introduced into the measuring instrument, A rotary encoder for detecting a rotation angle of the rotating optical fiber is provided in association with the rotating optical fiber, and the rotary encoder is connected to the measuring device, so that a light receiver corresponding to the optical fiber receiving the light from the rotating optical fiber. A sample liquid spectroscopic analysis characterized in that the measured signal from the element is selected, the peak value of the measured signal is compared with the reference signal, and the absorbance or fluorescence of the sample solution is measured. apparatus.