JPH06273703A - Agent for contact lens - Google Patents

Abstract

PURPOSE:To lessen the degradation in effect by an environmental change and to enable preservation over a long period of time while the effect is maintained by incorporating at least one of dodecyl sulfates and water-soluble org. mercapto group-contg. compds. as a protein-denaturating agent into the agent for contact lenses. CONSTITUTION:At least one of the dodecyl sulfates and the water-soluble org. mercapto group-contg. compds. are incorporated as the protein-denaturating agent into this agent for contact lenses. Such agent for contact lenses is used as a detergent, sterilizing agent, disinfectant or preserving agent. The dodecyl sulfates are exemplified by lithium dodecyl sulfate, sodium dodecyl sulfate, tris-dodecyl sulfate, triethanolamine dodecyl sulfate, isopropanol dodecyl sulfate, etc. On the other hand, the water-soluble org. mercapto group-contg. compds. are exemplified by 2-mercaptopropionic acid, 2-mercapto ethanol, 2- mercaptopropionyl glicine, 2-mercaptoethylamine hydrochlorate, etc.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an agent for contact lenses. The agent for contact lenses of the present invention is a soft contact lens, a cleaning agent for cleaning contact lenses regardless of hard contact lenses, a disinfectant for sterilizing contact lenses, a disinfectant for disinfecting contact lenses, or It can be used as a preservative for soaking contact lenses for a certain period of time.

[0002]

2. Description of the Related Art Conventionally, a method for cleaning contact lenses using a cleaning solution containing a protein-degrading enzyme as a main component has been known for removing proteinaceous stains adhering to contact lenses (JP-A-2- 168224).
This method involves diluting a contact lens cleaning stock solution consisting of a protein degrading enzyme, a water-miscible organic solvent, and water in an aqueous medium as a diluent, and dipping the contact lens in the resulting diluent. Then, the deposits on the lens surface are removed. Examples of protein-degrading enzymes include "Bioplase" (Nagase Seikagaku Co., Ltd.), "Alcalase", "Esperase", "Sabinase" (Novo Industry Japan KK), "Protease N".
"Amano" (Amano Pharmaceutical Co., Ltd.) and the like are used.

On the other hand, a protein denaturing agent is also used for cleaning contact lenses. As a cleaning agent for contact lenses using a substance falling within the category, urea and / or guanidinate and sulfite as a reducing agent are used. Pyrosulfite,
Those containing a dithionite salt, sodium arsenic hydride or a water-soluble organic mercapto group-containing compound have been reported (JP-A-54-56852). In this detergent, urea is used in an amount of 1 W / V% or more and a saturation concentration or less, and guanidinate is used in an amount of 3 W / V% or more and a saturation concentration or less.

[0004]

DISCLOSURE OF THE INVENTION Problems to be Solved by the Invention
The contact lens cleaning method using the protein degrading enzyme described in No. 224 requires the time and effort of mixing the undiluted cleaning solution containing the protein degrading enzyme with the diluent before use, and there is a possibility that one of the two solutions may be forgotten to be added. There is. Another problem is that protein-degrading enzymes cannot withstand long-term storage.

The above problem occurs because the cleaning solution for contact lenses contains protein degrading enzymes as a main component. Proteolytic enzymes cause changes in the environment, such as temperature changes, p
Its effectiveness is gradually lost due to changes in H, mixing of surfactants, etc. In order to maintain the stability of the protein degrading enzyme, it is necessary to dilute the former with the latter just before use, using a two-liquid type of a high-concentration protein degrading enzyme-containing stock solution and its diluent. It is also a drawback of protein degrading enzymes that the potency gradually decreases when stored for a long time.

Further, regarding the method of immersing a contact lens in a cleaning liquid containing urea and / or guanidinate as a protein modifier as described in JP-A-54-56852, the protein denaturing is performed to enhance the cleaning power. It is necessary to contain the agent in a high concentration. When the concentration of the protein denaturing agent is high as described above, the osmotic pressure value becomes too large, and when the lens is immersed for a long time, the lens diameter change, base curve change, power change, and other adverse effects on the lens may occur. . This adverse effect is greater in soft contact lenses than in hard contact lenses. In addition, it is necessary to thoroughly rinse off this solution when wearing it in order to prevent it from affecting the ocular mucosa.

The object of the present invention is to reduce the decrease in efficacy due to environmental changes, to save labor during use, and to preserve the effect for a long period of time.
It is an object of the present invention to provide a contact lens agent capable of exerting a high effect even at a low concentration of an active ingredient, for example, a cleaning agent, a bactericide, a disinfectant and a preservative.

[0008]

The agent for contact lenses of the present invention which achieves the above object is characterized by containing at least one of dodecyl sulfate and a water-soluble organic mercapto group-containing compound as a protein modifier. .

The present invention will be specifically described below. The agent for contact lenses of the present invention includes a cleaning agent, a germicide, a disinfectant,
Although it is used as a preservative, the cleaning agent will be described first.

The protein denaturant, which is the main component of the contact lens cleaning agent according to one embodiment of the present invention, acts on the protein stains attached to the contact lens to form a higher-order structure of the protein. Destroy. Therefore, for hard contact lenses, the protein stains on the lens surface are caught, and for soft contact lenses, the protein chains are caught or entangled not only on the lens surface but also on the polymer chains that make up the lens. Makes it easier to remove sexual stains.

The protein denaturing agent can be handled in the same manner as ordinary chemical substances, and does not undergo a significant decrease in its efficacy due to the environmental changes as seen in protein degrading enzymes. Therefore, it is not necessary to use the two-liquid type, and it is possible to store it for a longer time.

In the present invention, the protein modifier includes
At least one of dodecyl sulfate and a water-soluble organic mercapto group-containing compound is used. As dodecyl sulfate, lithium dodecyl sulfate, sodium dodecyl sulfate,
Dodecylsulfate tris, dodecylsulfate triethanolamine, dodecylsulfate isopropanol and the like can be mentioned, while water-soluble organic mercapto group-containing compounds include 2-mercaptopropionic acid, 2-mercaptoethanol, 2
-Mercaptopropionylglycine, 2-mercaptoethylamine hydrochloride and the like. As a result of judging from the washing effect, sodium dodecyl sulfate or 2-mercaptoethanol is particularly preferable. The preferred concentration of the protein denaturing agent is 0.001 to 0.05 (W / V)%
Is the range. The reason is that if it is less than 0.001%, the effect of the protein denaturing agent is small and the detergency becomes small, and if it exceeds 0.05%, the osmotic pressure becomes too large, and when the lens is immersed for a long time. This is because the influence on the lens shape such as the change of the lens diameter, the change of the base curve, and the change of the power becomes large. A more preferable concentration of the protein denaturing agent is 0.002 to 0.02 (W / V)%, and a particularly preferable concentration is 0.002 (W / V)% or more and 0.01 (W / V).
V) is less than%. The particularly preferable upper limit of the concentration of the protein denaturant is less than 0.01 (W / V)% is 0.01
This is because even if (W / V)% or more, the cleaning power is hardly further improved.

A buffer solution is used in the solution for dissolving the protein denaturing agent in order to adjust pH, osmotic pressure and the like. As the buffer solution, a phosphate buffer solution containing a mixed solution of sodium monohydrogen phosphate and sodium dihydrogen phosphate and a borate buffer solution containing a mixed solution of boric acid and borax are preferable. This is because these buffer solutions have been used for a long time as a component of a preservative solution for contact lenses and as a buffer solution for eye drops, and their safety has been well confirmed. Among them, the borate buffer solution is known to have a weak bactericidal activity and to be less contaminated by microorganisms during storage, and therefore is most suitable as a solution for dissolving a protein denaturing agent.

The preferred pH range of these buffers is 8.
It is 0 to 8.5. The cleaning effect of the agent for contact lenses of the present invention increases as it is biased toward alkali, but the pH range in which there is no irritation to the ocular mucosa is known to be 4.8 to 8.5, so the lower limit is 8.0. , And the upper limit is preferably 8.5.

As the osmotic pressure adjusting agent, sodium chloride, potassium chloride, butter sugar or the like may be contained. The preferred concentration of these modifiers will vary with other ingredients, but for example, with sodium chloride, 0.9 (W
/ V)% or less, potassium chloride can be 0.6 (W / V)% or less, and glucose can be 0.15 (W / V)% or less. When the concentration exceeds the above range, the osmotic pressure may increase.

Further, the preferable range of osmotic pressure having a small influence on the shape of the contact lens is 120 to 290 mO.
sm. In addition, for soft contact lenses, the effect can be enhanced by using a contact lens cleaning solution in which a protein denaturing agent is hypotonic, that is, a solution having a lower osmotic pressure than body fluid is used. For the lens, a hypotonic solution of 120-180 mOsm is desirable. Among them, especially 120-160
Most preferred is mOsm because of its high cleaning effect. With regard to soft contact lenses, by making them hypotonic, the chain of the polymer that constitutes the lens slightly expands, making it easier to remove stains. This slight chain spread can be easily restored by rinsing the lens with an isotonic solution prior to lens wear.

A surfactant may be added to the contact lens cleaning liquid in order to remove oily stains such as eye oil adhering to the contact lens. The surfactant should not affect the material, shape, etc. of the contact lens. It should not be adsorbed on the lens. Furthermore, it should be highly safe because it does not irritate the ocular mucosa. As those which meet the above conditions, for example, polyoxyethylene polyoxypropylene glycols, triethanolamine lauryl sulfates, alkyldiaminoethylglycines, polyoxynonylphenyl ethers, coconut oil fatty acid diethanolamides, dioctyl sodium sulfosuccinate, etc. Is used. These surfactants can be contained to the extent that the osmotic pressure is not increased.

In the cleaning method using the above cleaning agent, the contact lens is dipped in the cleaning agent and taken out after a certain period of time,
It is desirable to rinse it with tap water, physiological saline, or a commercially available storage solution before using it again. With respect to the detergent containing the surfactant, it is possible to perform scrubbing similarly to the ordinary contact lens cleaner.

The contact lens preparation of the present invention has been described above focusing on the cleaning agent. However, the contact lens preparation of the present invention has a sterilizing, disinfecting and preserving action for contact lenses. Also used as a disinfectant and preservative.

When used as a bactericide, disinfectant or preservative, the content of the protein modifier dodecyl sulfate and / or the water-soluble organic mercapto group-containing compound is the same as in the detergent, and the soft contact The osmotic pressure when used for a lens is the same as that for a cleaning agent.

[0021]

EXAMPLES The present invention will be described in more detail below with reference to examples. However, it goes without saying that the present invention is not limited to these examples.

First, a detergent containing the following protein modifier was prepared and its pH and osmotic pressure were measured. The measured value is
The results are shown in Table 1. In the components shown in Examples, 2-mercaptoethanol and sodium dodecyl sulfate were used as protein modifiers, sodium chloride was used as an osmotic pressure adjusting agent, and a phosphate buffer solution and a borate buffer solution were used as buffer agents. Poloxamer 188 and polyoxyethylene nonylphenyl ether were used as surfactants.

Example 1. 2-Mercaptoethanol 0.002 g Sodium chloride 0.04 g 0.05M phosphate buffer (pH 8.0) Appropriate amount 200 ml 0.05M phosphate buffer (pH 8.0) containing 2-mercaptoethanol in an amount of 0.2. 002 g, sodium chloride to 0.
It was mixed and prepared so as to be 04 g. 0.05M phosphate buffer is 0.05M sodium monohydrogen phosphate,
It was prepared by adding 0.05 M sodium dihydrogen phosphate until the pH reached 8.0.

Example 2. Sodium dodecyl sulfate 0.004 g Sodium chloride 0.04 g Poloxamer 188 (polyoxyethylene poly 0.02 g oxypropylene glycol) 0.05 M phosphate buffer (pH 8.0) Appropriate amount 200 ml 0.05 M phosphate buffer (PH8.0) 0.004 g of sodium dodecyl sulfate and 0.04 sodium chloride
4 g and poloxamer 188 were mixed to prepare 0.02 g. The 0.05M phosphate buffer was used in Example 1.
It was prepared in the same manner as.

Example 3. Sodium dodecyl sulfate 0.010 g 0.1 M phosphate buffer (pH 8.2 ) Appropriate amount 200 ml 0.1 M phosphate buffer (pH 8.2) was mixed with sodium dodecyl sulfate at 0.010 g. , Prepared. The 0.1M phosphate buffer solution is prepared by adding 0.1M sodium dihydrogen phosphate to 0.1M sodium dihydrogen phosphate at pH 8.
It was prepared by adding it to 2.

Example 4. Sodium dodecyl sulfate 0.010 g Polyoxyethylene nonylphenyl ether 0.02 g 0.1 M borate buffer (pH 8.5) Appropriate amount 200 ml 0.1 M borate buffer (pH 8.5) with sodium dodecyl sulfate It was prepared by mixing 0.010 g and 0.02 g of polyoxyethylene nonylphenyl ether. 0.
The 1 M borate buffer solution was prepared by adding 0.1 M boric acid to 0.1 M borax until the pH reached 8.5.

Example 5. Sodium dodecyl sulfate 0.02 g Distilled water Appropriate amount Total amount 200 ml 0.02 g of sodium dodecyl sulfate was mixed in distilled water to prepare a total amount of 200 ml.

Example 6. 2-Mercaptoethanol 0.016 g Sodium chloride 1.7 g Distilled water Appropriate amount 200 ml 2-Mercaptoethanol 0.016 g, Sodium chloride was mixed with distilled water to be 1.7 g, the total amount was 2
It was prepared to have a volume of 00 ml.

PH measurement pH was measured with a pH meter (F-8DP type, Horiba, Ltd.). Measurement of osmotic pressure It was measured using a freezing point depression method osmometer (VOGEL OM801).

[0030]

[Table 1] Method for evaluating cleaning effect An artificial soiling liquid consisting of albumin 0.2% and lysozyme chloride 0.2% was prepared, and a contact lens was immersed therein for 3 hours. After the immersion, the lens was dried at room temperature, and the turbidity was measured using a digital turbidimeter (NDH-1001DP manufactured by Nippon Denshoku Industries Co., Ltd.) (initial value).

The above lens was immersed in the cleaning agent prepared in each example overnight (15 hours). The lens was taken out the next day, dried at room temperature, and then measured for turbidity. The cleaning effect was evaluated by a value obtained by subtracting the initial value from this value (the higher the minus degree, the greater the cleaning effect). The results are shown in Table 2.

Evaluation of cleaning effect after long-term storage The cleaning effects prepared when the cleaning agents prepared in the respective examples were stored at 40 ° C. and 75% RH for 1 month or 2 months were compared. According to the method for evaluating the cleaning effect, the cleaning effect before storage (immediately after the preparation of the cleaning agent) was evaluated, and then the cleaning effect was similarly evaluated for the sample after 1 month storage and the sample after 2 months storage. The value before storage was set to 100% and comparison with the sample after storage was performed. A control detergent containing a protein-degrading enzyme was used and treated in the same manner as in the case of the detergent shown in the examples. The results are shown in Table 3.

The values of the preservative effect shown in Table 3 are expressed as follows, where β is the cleaning effect value before storage and 1 is the cleaning effect value after storage for 1 month or 2 months.

[Equation 1] Sought by.

[0034]

[Table 2] From Table 2, it was found that the turbidity value was smaller than the initial value in both the soft contact lens and the hard contact lens, showing a sufficient cleaning effect.

[0035]

[Table 3] From Table 3, it was found that the detergents of the respective Examples showed less reduction in efficacy after long-term storage than the control detergents using protein degrading enzyme. The cleaning agents for contact lenses obtained in Examples 1 to 6 were used as bactericides, disinfectants and preservatives, and excellent contact lens sterilization, disinfection and preservation effects were obtained.

[0036]

As described in detail above, by using the agent for contact lenses of the present invention, it has become possible to save the trouble of preparation which was a problem of the conventional method. In addition, it has become possible to preserve the effect for a long period of time. Furthermore, the agent for contact lenses of the present invention has a high effect even when the concentration of the active ingredient is low, and as a result, the osmotic pressure can be easily adjusted, and therefore the influence on the shape of the lens and the like can be reduced. Is possible.

Claims (4)

[Claims] 1. A contact lens agent comprising at least one of dodecyl sulfate and a water-soluble organic mercapto group-containing compound as a protein modifier. 2. The content of the protein modifier is 0.001 to
The amount is 0.05 (W / V)%.
The agent for contact lenses according to. 3. Used for soft contact lenses,
Osmotic pressure is 120-180 mOsm, The agent for contact lenses of Claim 1 or 2 characterized by the above-mentioned. 4. The agent for contact lenses according to claim 1, which is a cleaning agent, a bactericidal agent, a disinfectant or a preservative.