JPH06256126A - New microorganism, soil blight controlling agent containing the microorganism and soil blight controlling method using the agent - Google Patents

Abstract

PURPOSE:To provide a microbial strain giving little environmental pollution, compatible with ecosystem and having excellent stability and durability of the effect for controlling fusarium wilt, a fusarium wilt controlling agent containing the microbial strain and a method for controlling fusarium wilt using the agent. CONSTITUTION:The new microbial strain is Fusarium oxysporum JTF-108 (FERM BP-3964) and JTF-139 (FERM BP-3965) effective for controlling the fusarium wilt of carnation and tomato. The fusarium wilt controlling agent contains the JTF-108 strain and/or JTF-130 strain, a diluent inert to the properties of the strain and an assistant. The soil blight can be controlled by treating the root of a plant or the soil with the JTF-108 strain or JTF-139 grain.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a novel microorganism JTF-effective against Fusarium disease, which is a soil disease caused by Fusarium fungi, and in particular carnation wilt disease and tomato wilt disease.
108 strains and JTF-139 strains. The present invention also relates to a Fusarium disease control agent containing the JTF-108 strain and / or the JTF-139 strain, and a method for controlling Fusarium disease using these agents.

[0002]

2. Description of the Related Art Soil diseases caused by Fusarium fungi have been reported in many vegetables or flowers, and are one of the important diseases that hinder the stable production of crops. Also in carnation and tomato cultivation, carnation wilt disease and tomato wilt disease caused by Fusarium oxysporum are positioned as the most important diseases. Because
This is because carnations and tomatoes are cultivated throughout the year at the facility and are continuously cropped, so once a disease occurs, it leads to great damage.

As a means for controlling the disease caused by these Fusarium bacteria, soil disinfection using fumigants or steam has been indispensable. However, the use of fugitive agents with high toxicity causes many problems, such as causing environmental pollution or killing useful microorganisms and occupying pathogenic bacteria. On the other hand, as another control means, breeding of resistant varieties has been actively carried out. However, in flowers such as carnation, flower color, productivity and early growth,
Other traits are often prioritized in breeding,
Underutilized. In the case of tomatoes, among varieties with high marketability, there are no varieties resistant to wilt disease (race J2), and they are originally resistant varieties due to the differentiation of bacterial groups. Are always at risk of disease outbreak due to Fusarium.

[0004] Furthermore, studies have been conducted actively to select useful bacteria having an antagonistic action against pathogenic bacteria from the natural world and utilize them for biological control, and have been attracting attention as a means for disease control. There is. However, there are problems that the control effect is unstable, that there is only a short-term effect, or that the control effect can be obtained only with a limited use method, and those that can be practically used are extremely There were few.

[0005]

SUMMARY OF THE INVENTION The present invention has been made in view of the above circumstances, and its object is to reduce environmental pollution.
It is an object of the present invention to provide a microorganism which is in harmony with the ecosystem and is excellent in stability and sustainability of controlling effect against Fusarium disease, a Fusarium disease controlling agent containing the microorganism, and a method for controlling Fusarium disease using the same.

[0006]

In order to solve the above problems, the present inventors searched for natural enemy microorganisms effective for controlling soil diseases by Fusarium fungi. As a result, the novel microorganisms isolated from the root surface of the plant in the carnation cultivated area are carnation wilt disease (pathogenic fungus: Fusarium oxysporum Schl.f.sp.d), which is one of the soil diseases of carnation.
ianthi (Prill et Del.) Snyd.et Hans.) and tomato wilt disease (pathogen: Fusarium o), which is one of the soil diseases of tomato
xysporum Schl.f.sp.lycopersici (Sacc.) Snyd.et Hans.
It was found that the occurrence of race J2) was significantly suppressed.

That is, according to the present invention, a novel microorganism Fusarium oxysporum JTF-108 strain which is effective in controlling Fusarium disease of carnations and tomatoes and does not show pathogenicity to major crops (Microtechnology Research Institute 3964), and a new microorganism Fusarium oxysporum (Fusarium ox) which is effective in controlling Fusarium disease of carnations and tomatoes and has no pathogenicity to major crops.
Ysporum) JTF-139 strain (Microtechnology Research Institute Article No. 3965) is provided.

According to the present invention, the JTF-108 strain and / or
Alternatively, there is provided a Fusarium disease control agent containing a JTF-139 strain and, if necessary, a diluent that does not affect the properties of the strain and an auxiliary agent. Furthermore, JTF-108 strain or JT
Provided is a method for controlling Fusarium disease of carnations and tomatoes by treating F-139 strains in plant roots or soil.

The details of the present invention will be described below.

The microorganism of the present invention, JTF-108 strain and JT
The F-139 strain was obtained from the surface of the root of the carnation plant that was growing healthy in the carnation cultivated area, from the surface of Komoda medium (K ₂ HPO ₄ 1 g, KCl 500 mg, MgSO
_{4 · 7H 2 O 500mg, Fe} -EDTA 10m
g, L-asparagine 2 g, D-galactose 20
g, PCNB 75% wettable powder 1 g, Oxgol 5
00 mg, Na ₂ B ₄ O ₇ · 10H ₂ O 1 g, streptomycin sulfate 300 mg, agar 20 g, water 10
00 ml, pH 3.8-4.0) was used to separate a large number of Fusarium bacteria, and among the separated bacteria, a control effect against carnation wilt disease was shown, and eggplant, cucumber,
It was obtained by searching for strains that are not pathogenic to major crops such as cabbage or radish. Further, the microorganism of the present invention exhibits not only the control effect against carnation wilt disease but also the control effect against tomato wilt disease.

JTF-108 strain and JT which are microorganisms of the present invention
The F-139 strain has been deposited at the Institute of Microbial Science and Technology, the Agency of Industrial Science and Technology, on August 11, 1992, with the deposit numbers Mikokenjojo No. 3964 and No. 3965.

The microorganism of the present invention can be cultivated in a solid plate medium or slant medium such as potato dextrose agar medium (PDA medium) and Czapek agar medium.
It is also possible to perform shaking culture using a liquid medium for filamentous fungi or static culture.

The microorganism of the present invention does not show pathogenicity to other major crops such as carnation and tomato, and is safe for humans and livestock.

The microorganism of the present invention can be prepared as a soil disease controlling agent. This control agent can be combined with a diluent, an auxiliary agent, etc., if necessary.

The diluent used in the control agent of the present invention may be any of those usually used for agricultural chemicals as long as it does not affect the properties of the microorganism of the present invention. The diluent is used to dilute the microorganism to an appropriate concentration and facilitate the use, and may be solid or liquid. Which property is used can be selected depending on the dosage form of the control agent.
Distilled water can be preferably used as the liquid diluent, and talc can be preferably used as the solid diluent.

The auxiliary agent used in the control agent of the present invention does not affect the properties of the microorganism of the present invention,
Moreover, as long as it can enhance the control effect of the microorganism, any of those used for ordinary pesticides may be used. As the auxiliary agent, for example, a surfactant for increasing the dispersibility of microorganisms, a stabilizer for increasing the stability during storage, a sticking agent for increasing the spreadability and adhering well to crops. Examples of the agent, or synergist, which have no effect themselves but can increase the effect of controlling the microorganism of the present invention when used as a mixture, may be mentioned.

The control agent of the present invention may be in any form such as powder and wettable powder. The dosage form can be selected according to suitable conditions for manifesting the effect, the type and growth stage of the crop, the control method, and the like.

The microorganism of the present invention and the soil disease controlling agent containing the microorganism described above are used for controlling Fusarium disease, which is a soil disease caused by Fusarium bacterium, particularly carnation and wilt of tomato.

Fusarium disease can be controlled by treating the seedling roots of plants or soil with the microorganism of the present invention. As the treatment method, a plant seedling root dipping treatment,
Examples include plant seedling root dressing treatment, plant irrigation treatment, soil admixture treatment, and the like.

The plant seedling root dipping treatment can be carried out by immersing the plant seedling root portion in a microbial treatment liquid. The microbial treatment liquid can be obtained by culturing the microorganism of the present invention in a suitable liquid medium, and suspending the conidia-like cells obtained by filtering and centrifuging the culture liquid in a suitable liquid. Microbial suspensions are preferably used. The concentration of the microbial suspension is preferably in the range of 10 ⁴ to 10 ⁸ spores / ml,
Particularly preferred is 10 ⁶ to 10 ⁷ spores / ml. It is also possible to use, as the microorganism treatment liquid, a spore germination liquid obtained by germination of spores in the above-mentioned microorganism suspension and complete removal of bacterial cells.

The plant seedling root dressing treatment can be carried out by dressing the plant seedling root with microbial adsorbed talc. The microorganism-adsorbed talc is obtained by admixing the microorganism of the present invention and talc at a constant ratio to adsorb the microorganism, and naturally drying the mixture. The mixing weight ratio of microorganisms and talc is preferably in the range of 1: 1 to 1:10, and most preferably 1: 2. The concentration of microorganisms in the microorganism-adsorbing talc coated on the seedling root is 10 ³ to 10 ⁷
A range of spores / g is preferred, especially 10 ⁵ to 10 ⁶ spores /
A concentration of g is preferred.

The root irrigation treatment can be carried out when the root portion of the plant is planted in soil, that is, by irrigating the root with a microbial treatment solution at the time of planting. As the microbial treatment liquid, any one that can be used for the above-mentioned plant seedling root immersion treatment can be used. The plant root irrigation treatment can also be performed by inserting the ears before rooting into rooting soil, that is, by irrigating the plant root with a microbial treatment solution at the start of seedling raising. Furthermore, the control effect can be further enhanced by performing the treatment at both times.

The soil admixture treatment can be carried out by admixing the microorganism-adsorbing talc with the soil for planting the plant seedling roots during planting. As microbial adsorption talc,
The same thing as what was used for the above-mentioned plant seedling root dressing treatment can be used. The concentration of microorganisms mixed in the soil is 1
The range of 0 ⁴ to 10 ⁷ spores / g is preferable, and particularly 10 ⁶ to
A concentration of 10 ⁷ spores / g is preferred. This soil admixture treatment can also be performed by mixing microbial adsorbed talc with rooting soil for rooting the picked ears at the start of seedling raising. Further, it is also preferable to treat at both times in order to enhance the effect.

Thus, by treating the seedling roots of plants or soil with the microorganism of the present invention and the soil disease controlling agent containing the microorganism, Fusarium disease of carnation and tomato can be controlled.

[0025]

The microorganism of the present invention and the soil disease controlling agent containing the microorganism have excellent stability and sustainability of the controlling effect against Fusarium disease, especially carnation wilt disease and tomato wilt disease.

[0026]

EXAMPLES Examples of the present invention will be described below.

Example 1: Isolation and identification of JTF-108 and JTF-139 bacteria (1) Isolation of JTF-108 and JTF-139 bacteria; The roots of the plant bodies were collected from the carnations growing in. After removing the soil adhering to the surface of the collected root part as much as possible, 10 g of the root part was put into an Erlenmeyer flask containing 90 ml of sterilized water. After thoroughly shaking, the sterilized water was further diluted 10 ³ to 10 ⁵ times. After 0.1 ml of each concentration solution was added to and smeared on the previously described Komada medium, this was cultivated in a 28 ° C. incubator to separate the growing microorganisms. As a result of assaying the disease-controlling effect of the thus isolated microorganisms on carnation wilt disease, microorganisms JTF-108 and JTF-139 having excellent control effects and not showing pathogenicity to major crops were obtained. .

(2) Identification of JTF-108 and JTF-139 strains;
The microorganisms JTF-108 and JTF-139 isolated above were identified by identifying the microorganisms as potato dextrose agar medium (PDA medium: 200 g of potato tubers in the decoction liquid 100).
It was carried out by culturing in 0 ml, dextrose 20 g, agar 15 g), and investigating the elongation rate of mycelium, spore morphology and formation state.

In the potato-dextrose agar medium, the hyphae of both fungi grew rapidly, and large conidia and small conidia and many thick film spores were formed. The small conidia are O-diaphragm, colorless, elliptical, and pseudo-capillary formed on the short basidiomycota, which is lateral from the hyphae.
It was identified as Fusarium oxysporum by the Hansen and Hansen lookup tables.

(3) Properties of JTF-108 and JTF-139: <Growth status in each medium> PDA and Tuapec (N
aN0 ₃ 2.0 g, K ₂ HPO ₄ 1.0 g, MgS
_{O 4 · 7H 2 O 0.5g,} KCl 0.5g, FeS
O ₄ .7H ₂ O 0.01 g, sucrose 30.0 g, agar 15.0 g, water 1000 ml)
Both strains spread over the entire Petri dish (9 cm) on day 5 when cultured in an incubator at 0 ° C. The JTF-108 bacterium is a white fluffy flora, and when cultured in PDA medium for 10 days, the cultidia show a reddish purple color. On the other hand, JTF-139 is a white fluffy flora, but the cultivars are white. Both strains grow slightly faster on PDA medium than on Tuapec medium, and JTF-
108 bacteria also produce a large amount of pigment.

<Growth temperature and pH> The growth temperature of both strains is about 35 ° C. at the maximum and about 10 ° C. at the minimum, and the optimum temperature is 25 to 30 ° C. However, in comparison with JTF-108, JTF-139 has an extremely slow initial growth in the low temperature region of 10 ° C or 35 ° C.

Both strains can grow at a pH of 4.0 to 8.0, but the optimum pH is 6.0 to 7.0.

<Pathogenicity to Crops> Neither strain shows pathogenicity to major crops such as carnation, tomato, eggplant, cucumber, cabbage and radish.

<Production of mycotoxin> Fusarium graminearum Schwab
Trichothecene-type mycotoxins such as deoxynivalenol and nivalenol produced by e) and zearalenone are well known as causative bacteria causing health disorders in humans and animals. In addition, the above-mentioned mycotoxin production has been reported for other Fusarium bacteria. For example, Fusari
Regarding um oxysporum, there were few reports on mycotoxin production in the past, and there was a strong recognition that it was safe.
In recent years, Nivalenol, Fusarenone-X and T
The existence of strains that produce trichothecene-type mycotoxins such as -2 toxin and toxins such as zearalenone has been reported (WFO Marasas et al., 1984, TOXIGENIC FUS
ARIUM SPECIES, The Pennsiylvania State University
Press: 254-262).

Therefore, we asked Professor Yoshizawa of Kagawa University to investigate the toxin production performance of the novel microorganism of the present invention. This study was conducted with deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, 3,15-acetyldeoxynivalenol, nivalenol, fusarenone-X, 4,15-diacetylnivalenol, neosolaniol, HT-2 toxin and T. -2 toxin was analyzed by a gas chromatograph / mass spectrometer, and zearalenone was analyzed by a high performance liquid chromatograph. As a result, no toxin was detected,
It was confirmed that the novel microorganism according to the present invention is safe for humans and animals.

<Isozyme pattern of intracellular soluble esterase> Each of the various bacteria shown in FIG. 1 was statically cultured at 28 ° C. for 7 days in a potato dextrose liquid medium and a Tuapeck liquid medium. The mycelium thus obtained was treated with 0.05 M Tris-HCl buffer (pH 7.
After washing in 8), it was frozen. Then add this to 0.05
Triturate with M Tris-HCl buffer (pH 7.8), 1
Centrifugation was performed at 10,000 G, and the supernatant was used as a test material. Each of the test materials was electrophoresed at 15 mA using a polyacrylamide gel for pH 9.4. Enzyme activity staining is performed by Berry JA et al. (American J. Bot. 60: 976-9
86 (1973)) and performed by esterase staining. FIG. 1 shows the respective isozyme patterns thus obtained. As is clear from FIG. 1, the isozyme patterns of the novel microorganisms according to the present invention, namely JTF-108 and JTF-139, are Fusarium oxy.
sporum Schl.f.sp.dianthi), tomato wilt disease fungus (Fusari
um oxysporum Schl.f.sp.lycopersici race J1 and race
J2) and eggplant half-blight fungus (Fusarium oxysporum f.sp.me
The longenae) isozyme pattern was clearly different.

Example 2: Production of Fusarium control agent containing JTF-108 bacterium and JTF-139 bacterium (1) Liquid control agent Microorganisms JTF-108 bacterium and JTF-13 isolated in the above Example 1
Each of the 9 bacteria was mixed with potato dextrose liquid medium (PD
(Culture medium), shaking culture was carried out at 28 ° C. and 110 rpm for 5 days to obtain about 1-2 × 10 ⁸ spores / ml. This was filtered with gauze and centrifuged at 4000 rpm for 5 minutes, and then the precipitated conidia-like cells were treated with 10 ⁷ spores / ml.
A JTF-108 bacterial suspension was prepared by diluting with distilled water so that the concentration became. It can be used as it is as a fusarium control agent.

(2) Solid control agent The spores obtained by shaking culture and talc powder are adsorbed on talc powder by mixing them in a weight ratio of 1: 2, and then naturally dried. According to the procedure, a powdery fusarium control agent was prepared. This solid control agent can be stored for a long period of time and can be practically used as a disease control agent.

Example 3: JTF-108 fungus control test for carnation wilt by immersion treatment of roots of planted seedlings (1) Preparation of treatment liquid The liquid control agent for JTF-108 fungus produced in the above Example 2 was used. I was there. As a comparative example, benomyl wettable powder (active ingredient 50
%) Was diluted 1000 times with distilled water to obtain a benomyl-treated solution. Distilled water was used as a control.

(2) Immersion treatment Each treatment solution prepared in the above step (1) was poured into a beaker to a depth of 2 cm, and a carnation rooting part (variety: Lena) was put therein and immersed for 30 minutes. did.

(3) Preparation of Contaminated Soil Carnation wilt disease bacteria were cultivated with shaking in a potato dextrose liquid medium (PD medium) at 28 ° C. for 5 days. The obtained bacterial cells and talc were mixed at a weight ratio of 1: 2 to adsorb the bacterial cells to talc and then naturally dried. This was mixed with carnation soil (a mixture of vermiculite, Akatama soil and peat moss in a volume ratio of 4: 2: 2) to prepare a contaminated soil so that the pollution concentration was 10 ⁴ spores / g. .

(4) Planting 800 g of the contaminated soil prepared above was filled in a round plastic pot having a diameter of 18 cm and a depth of 20 cm, and the seedling treated in (2) was planted therein.

(5) Pest control effect test Cultivation was carried out by cultivating in a glass greenhouse at 28 ° C. for 3 months, and the condition of disease was investigated. The results are shown in Tables 1 and 2 below.

[0044]

[Table 1] The morbidity was calculated using the following formula.

Disease degree = (ΣDI × n / N × 4) × 100 DI: Disease index (see Table 2 below) n: Number of individuals showing the same DI N: Number of test individuals in each treatment group

[Table 2] From the results shown in Table 1 above, in the control group and in the benomyl-treated group, which is the most effective seedling treatment against Fusarium disease, 100% of the disease occurs and almost all the plants die, whereas JTF-108 It can be seen that a remarkable suppression effect is observed in the bacterium-treated section.

Example 4: JTF-139 fungus control test of carnation wilt by immersion treatment of roots of planted seedlings The liquid control agent containing the JTF-139 fungus prepared in Example 2 was used. Otherwise, the same procedure as in Example 3 was carried out to investigate the disease onset. The results are shown in Table 3 below. In Table 3, the standard of morbidity index and the calculation of morbidity were performed according to Table 1.

[0047]

[Table 3] As is clear from Table 3 above, 90% in the control area
In contrast to the high degree of illness and the high susceptibility, the JTF-139 treated group showed less illness and a remarkable illness control effect.

Example 5: Control effect test of carnation wilt disease by strain source irrigation treatment and soil admixture treatment of JTF-108 fungus control by strain source irrigation treatment and soil admixture treatment as compared with the effect by root root seedling root dipping treatment The effect was verified.

(1) Immersion Treatment for Rooting Seedling Roots The immersion treatment for rooting seedlings was carried out according to the methods of Examples 3 (1) to (4).

(2) Strain source irrigation treatment After planting carnation seedlings on the contaminated soil prepared by the method of Example 3 (3), JTF-10 prepared in Example 2 was used.
A liquid control agent containing 8 bacteria was irrigated at the source with 20 ml per strain.

(3) Soil admixture treatment The solid control agent containing the JTF-108 bacterium prepared in Example 2 was used in Example 3 so that the concentration was 10 ⁶ spores / g.
A soil admixture treatment was performed by mixing with the contaminated soil prepared by the method of (3).

(4) Pest control test Carnations treated by the above methods (1) to (3) were cultivated in a glass greenhouse at 28 ° C. for 3 months and Example 2
The onset situation was investigated by the same method. The results are shown in Table 4 below. The control was a root dipping seedling root dipping treatment using distilled water. The standard of the morbidity index and the calculation of the morbidity were performed according to Table 1.

[0053]

[Table 4] From Table 4 above, it can be seen that, in the strain irrigation treatment zone and the soil admixture treatment zone, a remarkable disease suppressing effect can be seen as in the immersion treatment zone.

Example 6: Test for controlling carnation wilt disease by JTF-108 fungus root dressing treatment and spore germination treatment In comparison with rooting seedling root soaking treatment effect, root dressing treatment and spore germination treatment The control effect was verified.

(1) Immersion treatment for rooted seedling root portion The immersion treatment for rooted seedling root portion was carried out according to the method of Example 3 (1) to (4).

(2) Root dressing treatment Root dressing treatment was carried out by dressing the roots of planted seedlings with the solid control agent (concentration: 10 ⁶ spores / g) containing the JTF-108 bacterium prepared in Example 2. I went.

(3) Treatment with spore germination solution The liquid control agent containing the JTF-108 bacterium prepared in Example 2 was thinly spread on a 9 cm sterilized petri dish, left at 25 ° C. for 48 hours, and then a 0.45 μm Millipore filter was used. The microbial cells were completely removed through to prepare a spore germination solution. A spore germination solution treatment was carried out by immersing the root part of the rooted seedling in the solution for 30 minutes. The treated seedlings were planted in a pot containing the contaminated soil prepared by the method of Example 3 (3).

(4) Control effect test The carnation treated by the above methods (1) to (3) was cultivated in a glass greenhouse at 28 ° C. for 3 months, and the disease state was investigated by the same method as in Example 3. did. The results are shown in Table 5 below. The control was a root dipping seedling root dipping treatment using distilled water. The standard of the morbidity index and the calculation of the morbidity were performed according to Table 1.

[0059]

[Table 5] From the results in Table 5 above, it can be seen that, in the root dressing-treated group and the spore germination solution-treated group, the disease-inhibiting effect can be seen as in the root-dipping-treated group.

Example 7: Carnation Wilt Disease Control Effect Test by Treatment of JTF-108 Fungus with Planting and Raising Seedlings Carnation is picked from a mother plant, inserted into rooting soil, and rooted for about 3 weeks. Therefore, it is common to grow seedlings. Therefore, when raising seedlings JTF-10
It was verified whether the control effect was exhibited after planting by treating 8 bacteria.

(1) Immersion Treatment for Rooting Seedling Roots The immersion treatment for rooting seedlings was carried out according to the methods of Examples 3 (1) to (4).

(2) Mixing Treatment for Rooting Soil In Example 2, the rooting soil (perlite and metromix mixed at a volume ratio of 7: 3) at the time of rooting work for growing planted seedlings was used. The concentration of the prepared solid control agent is 10 ⁶
Mix to give spores / g. Roots were grown in this soil for about 3 weeks to grow seedlings.

(3) Treatment of roots at the time of rooting After the roots were inserted into the rooting soil, the liquid control agent prepared in Example 2 was irrigated to the roots of the heads in an amount of 5 ml per strain to grow seedlings. did.

(4) Control effect test The seedlings grown by the above methods (1) to (3) were planted in a pot containing the contaminated soil prepared by the method of Example 3 (3) and kept at 28 ° C. It was cultivated in a glass greenhouse for 2 months, and the disease state was investigated by the same method as in Example 3.
The results are shown in Table 6 below. The control was a rooting seedling root immersion treatment using distilled water. The standard of the morbidity index and the calculation of the morbidity were performed according to Table 1.

[0065]

[Table 6] From the results in Table 6 above, it can be seen that the control effect can be obtained not only by treating rooted seedlings but also by treatment during seedling raising.

Example 8: Control effect test of JTF-108 bacterium and JTF-139 bacterium against tomato wilt disease (race J2) (1) Preparation of treatment liquid Liquid control containing JTF-108 bacterium prepared in Example 2 The agent and a liquid control agent containing JTF-139 bacteria were used. Distilled water was used as a control treatment liquid.

(2) Immersion treatment Each of the above treatment solutions was poured into a beaker to a depth of 2 cm, and the tomato seedling root portion (variety: Momotaro) at the 5th leaf stage 50 days after seeding had passed. And soaked for 30 minutes.

(3) Preparation of contaminated soil Tomato wilt disease fungus (race J2) was shake-cultured in a potato dextrose liquid medium (PD medium) at 28 ° C. for 5 days. The obtained bacterial cells and talc were mixed at a weight ratio of 1: 2 to adsorb the bacterial cells to talc, and then naturally dried. This was mixed with soil to prepare a contaminated soil so that the contaminated concentration was 10 ³ spores / g.

(4) Planting 800 g of the contaminated soil prepared above was filled in a round plastic pot having a diameter of 18 cm and a depth of 20 cm, and the seedling treated in the above (2) was planted therein.

(5) Pest control effect test Cultivation was carried out for 2 months in a glass greenhouse at 28 ° C., and the disease state was investigated. The results are shown in Table 7 below.

[0071]

[Table 7] The degree of morbidity was calculated according to the method already described in Table 1, and the morbidity index used was that shown in Table 2.

From Table 7 above, the control plots were almost 100% sick and the morbidity was high, while JTF-108 and JTF-13 were infected.
It can be seen that the 9-bacteria treated section has few disease-causing strains and exhibits a remarkable disease-control effect.

Example 9: Investigation of pathogenicity of JTF-108 bacterium and JTF-139 bacterium to other crops Using the same treatment solution as in Example 8 (1), 26 species of plants in 15 families shown in Table 8 below. A pathogenicity survey was conducted by the following method.

[0074]

[Table 8] First, for crops other than spinach and rice, these seedling roots were immersed in the above-mentioned treatment solution for 30 minutes and then planted. On the other hand, for spinach and rice, 20 ml of each of the above treatment solutions was used for seedling seedlings two weeks after seeding in pots, and the seedlings were irrigated.

The seedlings treated by each method were cultivated in a greenhouse at 25 ° C., and the growth conditions were investigated thereafter. As a result, in both plants, both strains showed the same growth condition as the control group (water treatment), It was found that it did not show pathogenicity.

[0076]

EFFECTS OF THE INVENTION When the microorganism of the present invention and the soil disease controlling agent containing the microorganism are used, it does not pollute the environment or affect human livestock or other crops, and thus harm the harmony of the ecosystem. It is possible to control carnation and fusarium disease of tomato stably and continuously without any disease.

Further, by using the microorganism of the present invention and the soil disease controlling agent containing the microorganism, even if the use of the soil disinfectant, which is feared to affect the environment, is reduced, Cultivation becomes possible.

Further, by using the control method of the present invention, disease control can be effectively performed without killing beneficial microorganisms.

[Brief description of drawings]

FIG. 1 is a diagram showing the isozyme patterns of the microorganisms JTF-108 and JTF-139 of the present invention in comparison with the isozyme patterns of various wilt disease fungus and eggplant half-blight fungus.

Claims (4)

[Claims] 1. A novel microorganism Fusarium oxysporu which is effective for controlling Fusarium disease of carnations and tomatoes and has no pathogenicity to major crops.
m) JTF-108 strain (Microtechnology Research Institute No. 3964). 2. A novel microorganism Fusarium oxysporu effective for controlling Fusarium disease of carnations and tomatoes and having no pathogenicity to major crops.
m) JTF-139 strain (Ministry of Industrial Science and Technology, Article 3965). 3. A fusarium disease control agent containing the JTF-108 strain according to claim 1 and / or the JTF-139 strain according to claim 2. 4. A method for controlling Fusarium disease of carnation and tomato by treating the JTF-108 strain according to claim 1 and / or the JTF-139 strain according to claim 2 in the root part or soil of a plant.