JPH0618461A - Monitoring method for activity of ion oxidizing bacteria - Google Patents
Monitoring method for activity of ion oxidizing bacteriaInfo
- Publication number
- JPH0618461A JPH0618461A JP17295392A JP17295392A JPH0618461A JP H0618461 A JPH0618461 A JP H0618461A JP 17295392 A JP17295392 A JP 17295392A JP 17295392 A JP17295392 A JP 17295392A JP H0618461 A JPH0618461 A JP H0618461A
- Authority
- JP
- Japan
- Prior art keywords
- electrodes
- double layer
- activity
- oxidizing bacteria
- layer capacity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、鉱石のバクテリア・リ
ーチングに際して用いられる鉄酸化細菌を含む培養液の
活性度をモニタリングする方法に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for monitoring the activity of a culture solution containing iron-oxidizing bacteria, which is used for bacterial leaching of ores.
【０００２】[0002]
【従来の技術】鉄酸化細菌を含む培養液の活性は、効率
（生産性）に大きく影響することから、その活性度を定
期的にモニタリングすることが必要である。2. Description of the Related Art Since the activity of a culture solution containing iron-oxidizing bacteria greatly affects the efficiency (productivity), it is necessary to regularly monitor the activity.
【０００３】従来のモニタリング方法としては、（１）
鉄酸化細菌の増殖速度を経験的にとらえることによって
培養液の交換を行う方法、（２）培養液のｐＨ，イオン
の分析（ｐＨ，Ｆｅ２＋，ＳＯ４ ２−１等）を行う方法
が知られている。The conventional monitoring method is (1)
A method of exchanging the culture solution by empirically grasping the growth rate of iron-oxidizing bacteria, and (2) a method of analyzing the pH and ion of the culture solution (pH, Fe 2+ , SO 4 2-1 etc.) are known. Has been.
【０００４】[0004]
【発明が解決しようとする課題】しかし、上記モニタリ
ング方法においては、次のような欠点がある。However, the above-mentioned monitoring method has the following drawbacks.
【０００５】ａ．上記（１）の方法は、必ずしも正確で
はなく、又経験が必要である。A. The above method (1) is not always accurate and requires experience.
【０００６】ｂ．上記（２）の方法は、時間と手数がか
かる。B. The above method (2) requires time and trouble.
【０００７】本発明の目的は、上記ａ、ｂの欠点を有し
ないモニタリング方法を提供することである。An object of the present invention is to provide a monitoring method that does not have the above-mentioned drawbacks a and b.
【０００８】[0008]
【課題を解決するための手段】本発明に係る鉄酸化細菌
の活性度をモニタリングする方法は次のとおりである。A method for monitoring the activity of iron-oxidizing bacteria according to the present invention is as follows.
【０００９】鉄酸化細菌を含む培養液中に、２枚の白金
板から成る電極を浸漬し、この２枚の電極間に１０ｋＨ
ｚから１０Ｈｚまでの正弦波電圧を印加して求まるイン
ピーダンス軌跡から電気二重層容量を算出し、この容量
の大きさから鉄酸化細菌の活性度をモニタリングする方
法。An electrode composed of two platinum plates was immersed in a culture solution containing iron-oxidizing bacteria, and 10 kH was applied between the two electrodes.
A method of calculating the electric double layer capacity from an impedance locus obtained by applying a sine wave voltage from z to 10 Hz, and monitoring the activity of iron-oxidizing bacteria from the size of the capacity.
【００１０】上記構成において、電極として用いられる
白金は不溶性であり、かつ反応活性が高いため、本発明
に用いる電極用としては最も好ましい金属である。印加
する１０ＫＨｚ〜１０Ｈｚの電圧は電気二重層容量を求
めるのに十分なインピーダンス軌跡を得るためである。In the above structure, platinum used as an electrode is insoluble and has high reaction activity, and is the most preferable metal for the electrode used in the present invention. The voltage of 10 KHz to 10 Hz applied is for obtaining an impedance locus sufficient for obtaining the electric double layer capacitance.
【００１１】[0011]
【作用】培養液は適当な液槽内に入れ、これに２枚の電
極を同じ条件にて浸漬する。そして、ＡＣインピーダン
スメーターから２枚の電極間に１０ｋＨｚから１０Ｈｚ
までの正弦波電圧（１０ｍＶｒｍｓ）を印加して、イン
ピーダンス軌跡を求める。次に、このインピーダンス軌
跡から電気二重層容量を算出する。鉄酸化細菌の活性が
高い程、つまり電極に対する鉄酸化細菌が付着するほど
電気二重層容量が大きくなるので、この容量の大きさに
より、鉄酸化細菌の活性度のモニタリングが可能であ
る。なお、モニタリングは連続して行ってもよいが、通
常は２４時間毎に一回位でもよい。The culture solution is placed in a suitable liquid tank, and two electrodes are immersed in the same solution under the same conditions. And 10kHz to 10Hz between the AC impedance meter and the two electrodes
The sine wave voltage (10 mVrms) up to is applied to obtain the impedance locus. Next, the electric double layer capacitance is calculated from this impedance locus. The higher the activity of the iron-oxidizing bacterium, that is, the more the iron-oxidizing bacterium adheres to the electrode, the larger the electric double layer capacity. Therefore, the activity of the iron-oxidizing bacterium can be monitored by the size of this capacity. The monitoring may be carried out continuously, but usually it may be carried out once every 24 hours.
【００１２】[0012]
【実施例】図１にモニタリング装置を示す。１は培養液
槽にして、この培養液槽１には鉄酸化細菌を含む培養液
２が入っている。３、３ａは２枚の白金板から電極にし
て、この電極３、３ａはホルダー４により同一条件（露
出面積が同一、同じ形状、同じ厚さ）で培養液槽１に垂
直に固定されている。５はＡＣインピーダンスメーター
にして、このＡＣインピーダンスメーター５は前記電極
３、３ａに対して１０ｋＨｚから１０Ｈｚまでの正弦波
電圧が印加できる。６は応答正弦波検出回路、７は電気
二重層容量演算回路にして、この回路７により算出され
た活性度は表示回路８に表示される。EXAMPLE FIG. 1 shows a monitoring device. Reference numeral 1 denotes a culture solution tank, and the culture solution tank 1 contains a culture solution 2 containing iron-oxidizing bacteria. 3 and 3a are electrodes from two platinum plates, and the electrodes 3 and 3a are vertically fixed to the culture solution tank 1 by the holder 4 under the same condition (exposed area is the same, same shape, same thickness). . 5 is an AC impedance meter, and this AC impedance meter 5 can apply a sine wave voltage of 10 kHz to 10 Hz to the electrodes 3, 3a. 6 is a response sine wave detection circuit, 7 is an electric double layer capacitance calculation circuit, and the activity calculated by this circuit 7 is displayed on the display circuit 8.
【００１３】図２に経時的な電気二重層容量の変化を示
す。FIG. 2 shows the change in electric double layer capacity over time.
【００１４】この図２から明らかなように、無菌状態で
は１０００μＦ／ｃｍ２位であるが、１０６セル／ｍｌ
生息していると３０００μＦ／ｃｍ２を超えることが判
る。また、鉄酸化細菌が増殖すると電気二重層容量の値
は増加の一途をたどる。As is clear from FIG. 2, it is about 1000 μF / cm 2 in a sterile state, but 10 6 cells / ml.
It can be seen that the habitat exceeds 3000 μF / cm 2 . When the iron-oxidizing bacteria grow, the value of electric double layer capacity keeps increasing.
【００１５】[0015]
【本発明の効果】本発明は以上のように、白金板から成
る電極間に１０ｋＨｚ〜１０Ｈｚの正弦波電圧を印加
し、この応答正弦波電流を測定することにより、電気二
重層容量の大きさを算出できるので、この容量の大きさ
により、鉄酸化細菌の活性度を極めて簡単かつ迅速に、
そして正確に知ることができる。この結果、効率的なバ
クテリア・リーチングを行うことができる。As described above, the present invention applies the sine wave voltage of 10 kHz to 10 Hz between the electrodes made of platinum plates and measures the response sine wave current to determine the magnitude of the electric double layer capacitance. Therefore, the activity of iron-oxidizing bacteria can be extremely easily and quickly determined by the size of this capacity.
And you can know exactly. As a result, efficient bacterial leaching can be performed.
【図１】本発明に係るバクテリア・リーチングに使用さ
れている鉄酸化細菌の活性度をモニタリングする方法を
実施するための装置の説明図。FIG. 1 is an explanatory view of an apparatus for carrying out a method for monitoring the activity of iron-oxidizing bacteria used for bacterial leaching according to the present invention.
【図２】経時的な電気二重層容量の変化の説明図。FIG. 2 is an explanatory diagram of changes in electric double layer capacity over time.
１ 培養液槽 ２ 培養液 ３、３ａ 電極 ４ ホルダー ５ ＡＣインピーダンスメーター ６ 応答正弦波電流測定回路 ７ 電気二重層容量演算回路 ８ 表示回路 1 culture liquid tank 2 culture liquid 3, 3a electrode 4 holder 5 AC impedance meter 6 response sine wave current measurement circuit 7 electric double layer capacity calculation circuit 8 display circuit
Claims (1)
金板から成る電極を浸漬し、この２枚の電極間に１０ｋ
Ｈｚから１０Ｈｚまでの正弦波電圧を印加して求まるイ
ンピーダンス軌跡から電気二重層容量を算出し、この容
量の大きさから鉄酸化細菌の活性度をモニタリングする
方法。1. An electrode composed of two platinum plates is immersed in a culture solution containing iron-oxidizing bacteria, and 10 k is placed between the two electrodes.
A method of calculating an electric double layer capacity from an impedance locus obtained by applying a sinusoidal voltage of 10 Hz to 10 Hz, and monitoring the activity of iron-oxidizing bacteria from the size of the capacity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17295392A JPH0618461A (en) | 1992-06-30 | 1992-06-30 | Monitoring method for activity of ion oxidizing bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17295392A JPH0618461A (en) | 1992-06-30 | 1992-06-30 | Monitoring method for activity of ion oxidizing bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0618461A true JPH0618461A (en) | 1994-01-25 |
Family
ID=15951428
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP17295392A Pending JPH0618461A (en) | 1992-06-30 | 1992-06-30 | Monitoring method for activity of ion oxidizing bacteria |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0618461A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002330752A (en) * | 2001-05-08 | 2002-11-19 | Sanden Corp | Apparatus for counting number of microorganisms |
-
1992
- 1992-06-30 JP JP17295392A patent/JPH0618461A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002330752A (en) * | 2001-05-08 | 2002-11-19 | Sanden Corp | Apparatus for counting number of microorganisms |
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