JPH0616558Y2 - Cell culture device - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION <Industrial field of application> The present invention relates to an apparatus for culturing cells by stirring a cell culture solution in a tank.

<Prior Art> In a conventional cell culture device, as shown in FIG. 2, a culture solution b containing cells such as plants is placed in a tank a, and the culture solution b is stirred by a stirring blade c. There are those that perform culturing.

<Problems to be Solved by the Present Invention> The conventional cell culture device has the following problems.

That is, when the culture fluid b is stirred by the stirring blade c, the liquid surface of the culture fluid b is wavy due to the centrifugal force due to the rotation of the stirring blade c and the generation of bubbles due to aeration, and the cells adhere to the inner surface of the tank a. I will end up.

Therefore, the adhered cells die and remain as a carcass d on the inner surface of the tank a.

Therefore, in the conventional cell culture device, the production efficiency of the cells in the tank a is reduced by the amount of the cells that are killed.

<Purpose of the present invention> The present invention has been made to solve the above problems, and prevents cells from adhering to the inner surface of the tank caused by agitation, reduces the number of dead cells, and produces cells. An object is to provide a cell culture device capable of increasing efficiency.

<Structure of the Invention> Hereinafter, an embodiment of the invention will be described with reference to the drawings.

<A> Tank The tank 1 is a sealed container for storing the culture solution 4 of cells such as plants.

A rotary shaft 11 is attached to the axial center of the tank 1 in the vertical direction.

At the lower end of the rotary shaft 11 inside the tank 1, the stirring blade 1
2 is attached, and the tank 1 is attached to the upper end of the rotary shaft 11.
Is connected to the rotary drive motor 13 outside.

A known air filter 14 is attached to the tank 1.

<B> Screw The screw 2 has a mounting hole for the rotating shaft 11 at the center of the shaft.
Install above 2.

Therefore, the screw 2 is configured to be rotatable around the rotating shaft 11.

<C> Cylindrical Body The cylindrical body 3 surrounds and fixes the peripheral portion of the screw 2 from the upper end to the lower end of the screw 2.

Both upper and lower end surfaces of this cylindrical body 3 are open.

<D> Umbrella-shaped member The umbrella-shaped member 31 has an umbrella-like shape that is inclined downward from the upper end peripheral portion of the cylindrical body 3 and reaches a little near the inner peripheral surface of the tank 1.

The distance between the lower end of the umbrella upper member 31 and the inner surface of the tank 1 is set such that the culture solution 4 flowing down on the umbrella-shaped member 31 adheres along the inner surface of the tank 1 and further flows downward.

Further, the umbrella-shaped member 31 is formed so that its lower end is located at least slightly above the lower end of the tubular body 3.

<Operation of the Present Invention> Next, the operation of the present invention will be described.

First, the cell culture solution 4 is placed in the tank 1.

At this time, the height of the liquid surface of the culture solution 4 is injected so as to be located at least below the distance L from the lower end of the umbrella-shaped member 31.

The distance L is a height at which cells may attach to the inner surface of the tank 1 due to the shaking of the liquid surface of the culture solution 4.

First, when the motor 13 is operated and the rotary shaft 11 is rotated, the stirring blade 12 is rotated and the culture solution 4 in the tank 1 is stirred.

At the same time, the screw 2 rotates, and the culture solution 4 becomes the cylindrical body 3.
Rise inside.

At this time, the rotating shaft 11 needs to be rotated in a direction in which the screw 2 can pump up the culture solution 4.

The raised culture medium 4 flows out in all directions from the upper end opening of the cylindrical body 3 and flows down along the upper surface of the umbrella-shaped member 31.

The culture solution 4 that has reached the inner surface of the tank 1 further flows downward from the gap between the inner surface of the tank 1 and the lower end portion of the umbrella-shaped member 31, and the distance L at which cells may be attached to the inner surface of the tank 1. While adhering thereto, it reaches the liquid surface of the culture solution 4.

Therefore, a fluidized film of the culture solution 4 is formed on the inner surface portion of the tank 1 where cells may be attached.

Therefore, even if the liquid surface of the culture solution 4 is shaken by stirring, it is possible to prevent cells from adhering to the inner surface of the tank 1 by the fluid film.

<Effects of the Present Invention> Since the present invention has been described above, the following effects can be expected.

<B> The conventional cell culture device uses the centrifugal force generated by the rotation of the stirring blades and the generation of bubbles due to aeration.
The liquid surface of the culture liquid becomes wavy, and cells adhere to the inner surface of the tank.

Therefore, the attached cells die, and the production efficiency of the cells in the tank is reduced by the amount of the dead cells.

On the other hand, the device of the present invention is configured such that the culture solution in the tank is agitated and at the same time, the culture solution is flowed through the screw and the umbrella-shaped member along the inner surface portion of the tank where cells may adhere. is there.

Therefore, it is possible to prevent cells from adhering to the inner surface of the tank.

Therefore, the dead cells attached to the inner surface of the tank can be eliminated, and the production efficiency of the cells in the tank can be increased accordingly.

<B> The device of the present invention can be rotated by attaching the stirring blade and the screw to the same rotary shaft.

Therefore, adhesion of cells can be prevented by using the same power as that for stirring the culture solution, which is economical.

<C> By allowing the culture solution to flow on the upper surface of the umbrella member, the opportunity for the culture solution to contact the air layer increases.

Therefore, the amount of dissolved oxygen in the liquid can be increased,
It can promote the growth of cultured cells.

[Brief description of drawings]

 FIG. 1: Illustration of one embodiment of the present invention FIG. 2: Illustration of prior art

Claims (1)

[Scope of utility model registration request] 1. A cell culture device comprising a tank for storing a cell culture solution, a rotary shaft vertically positioned in the tank, and a stirring blade attached to the rotary shaft, wherein a stirring blade above the rotary shaft is provided. Attached to the screw and rotating around the rotation axis, a cylinder surrounding the periphery of the screw and attached, and tilted downward from the vicinity of the upper end of the cylinder, slightly in front of the inner peripheral surface of the tank. A cell culture device comprising an umbrella-shaped member reaching up to.