JPH0568430A - Method for culturing lentinus edodes in mushroom bed - Google Patents

Abstract

PURPOSE:To solve difficulty in acquisition of sawdust and to culture Lentinus edodes in an mushroom bed by packing a medium using bagasse as a medium base and bran as a nutrient additive into a container, sterilizing, inoculating spawn, culturing a mushroom bed, spreading the mycelia, aging and immersing the mushroom bed in water. CONSTITUTION:Bagasse is used as a medium base and bran as a nutrient additive, 36-45wt.% bagasse is mixed with 12-18wt.% bran and 43-46wt.% water to prepare a medium. The medium is packed into a container made of polypropylene, sterilized under high pressure at 120 deg.C for 90 minutes, allowed to stand, chilled to about 18 deg.C by a cooler, spawn of Lentinus edodes is inoculated to the medium in a clear inoculating chamber free from various germs and the cells are cultured in a culture chamber in a darkroom at 18 deg.C room temperature at 70% humidity for 30 days. The mycelia are spread in the whole medium, then the mycelia are cultured at 22 deg.C room temperature and at 70% humidity under 600 lux for 60 days, the medium is aged, fruit body primordial are formed in a coating film of the mushroom bed, the mushroom bed is immersed in water at 28 deg.C for 24 hours and fruit bodies are produced and grown to culture Lentinus edodes in the mushroom bed.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for cultivating a mushroom bed of shiitake mushrooms, and in particular, a method for cultivating shiitake mushrooms in a mushroom bed for effectively utilizing unused resources by using bagasse as a substitute substrate for sawdust used in a medium. It is about the method.

[0002]

2. Description of the Related Art Shiitake mushrooms are cultivated by using broad-leaved trees such as oak and cypress, which are cut into 1m-long pieces that are convenient for handling. It is placed under an artificial shade or under the artificial shade, and the hyphae of the shiitake mushroom are grown on the log. By doing this, in the second year, shiitake mushrooms would grow on the logs and be harvested. Recently, in order to eliminate the depletion of raw wood for sardines, reduce the amount of work due to the aging of producers, shorten the cultivation period, etc., instead of raw wood cultivation, a container using a fungal bed mainly composed of hardwood sawdust Cultivation (bag cultivation, bottle cultivation) has come to be carried out, and the production amount thereof is increasing. That is, a medium is prepared by mixing hardwood sawdust such as oak and syrup, a nutrient such as rice bran, and water, and the medium is filled in a heat-resistant bag or polypropylene bottle and sealed with a cap using a filter. The bacteria in the medium are subjected to high-pressure sterilization treatment, the inoculum is inoculated into the medium in a clean inoculation chamber, and the medium inoculated with the inoculum, that is, the bed is cultured in the culture chamber.

[0003]

Recently, especially for hardwood sawdust, the amount of sawmill materials received has decreased, and with the increase in mushroom production, it has become difficult to secure sawdust for medium every year. Therefore, the price of sawdust is increasing year by year, and the production cost of shiitake mushrooms is also increasing accordingly.

The present invention has been made in order to solve the above-mentioned problems of the prior art, and is an unused resource by using bagasse as a substitute base material for hardwood sawdust, which is a raw material for shiitake mushroom bed cultivation. It is an object of the present invention to provide a method of cultivating a mushroom bed of shiitake mushrooms that effectively utilizes bagasse and at the same time solves the difficulty of obtaining sawdust and reduces the production cost of shiitake mushrooms.

[0005]

In order to achieve the above object, the method for cultivating a mushroom bed of shiitake mushrooms according to the present invention comprises a medium filled in a container, sterilized and inoculated with an inoculum, and the bacterial bed is cultivated. In the method of cultivating a mushroom bed of shiitake mushrooms that causes fruiting bodies to grow and grow by immersing them in a fungus bed after aging, cultivating the fungus, and aging, the medium base material is bagasse and nutrient additives are used to create the medium. Is. In particular, the composition of the medium is bagasse 36-45%, bran 12-18%, water 43-46%.
The water content in the medium is about 6 by weight.
It is 5%.

[0006] That is, the present invention, in a sugar factory,
Bagasse, which is a sugarcane pomace that has no use other than as a direct fuel for factories, is used as a medium base material, and as a nutritional additive, it is a skin residue produced when wheat is ground into flour. A culture medium is prepared using bran and the shiitake mushroom is cultivated.

[0007]

[Operation] First, the medium is prepared by sieving bagasse to make the particle size uniform, and the adjusted bagasse, bran and water are mixed in a weight ratio of 36 to 45%, 12 to 18% and 43 to 46, respectively.
It is mixed so that it becomes a ratio of%. At this time, the water content in the medium is about 65%. Further, the hydrogen ion index (PH) of the medium is around 5.5, which is a favorable value for the growth of shiitake mushrooms.

[0008]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Next, each embodiment of the present invention will be described with reference to FIGS. FIG. 1 is a process diagram showing a method for cultivating a mushroom bed of shiitake mushrooms according to an embodiment of the present invention, and FIG. 2 is an explanatory diagram showing the composition of a medium used in the experiment of the present invention and the yield of the experiment result. First, an example in which the effect of the experiment of the present invention is greatest will be described with reference to FIG. In the following description of the steps, the step numbers shown in FIG. First, add 5 to 10 m of bagasse with a water content of 40 to 45%.
Sieve through an m-mesh screen to even out the particle size. Then, bran with a water content of about 10 to 15% is prepared. Bagasse, bran, and water are mixed at a weight ratio of 42%, 14%, and 44%, respectively. At this time, the medium water content is 65%, the medium P
H becomes about 5.5 (step 1). This medium composition is the third from the top in the composition table of FIG.

1 kg of this medium is packed in a polypropylene bag, sealed with a filter cap (step 2), and high-pressure sterilized at 120 ° C. for 90 minutes (step 3). The sterilized medium is placed in a cooling chamber, and the medium is cooled to about 18 ° C. using a refrigerator (step 4). After cooling, the inoculum is inoculated in a clean inoculation room free of germs (step 5). The medium inoculated with the inoculum is cultured in the culture room (step 6). In the first half of the culture, the cells are cultured for 30 days in a dark room with a room temperature of 18 ° C. and a humidity of 70%, and mycelia are spread over the entire medium (step 6-1). Room temperature 2 in the latter half of culture
It is cultured for 60 days at 2 ° C., 70% humidity and 600 lux illuminance, and the medium is aged to form the primordia of the fruiting bodies in the membrane of the bacterial bed (step 6-2).

After completion of the culture, remove the fungal bed from the bag and
Immerse in 8 ° C. water for 24 hours (step 7). After the water immersion treatment, fruiting bodies are generated in a generation chamber having an indoor temperature of 18 ° C., a humidity of 85% and an illuminance of 500lux (step 8). It is harvested about 10 days after it is placed in the generating room (step 9). As shown in FIG. 2, the yield is 140 g per 1 kg of the medium, that is, 14% based on the weight of the medium. This ratio is almost the same as the value when hardwood sawdust is cultivated as a medium substrate.

Although the above-mentioned embodiment has explained an example in which the effect of the experiment according to the present invention is the greatest, other experimental data are also shown in FIG. FIG. 2 shows a table of medium composition and the amount of harvest obtained as a result of bacterial bed cultivation with the medium of each composition in medium 1k.
The graph is also shown together with a bar graph showing the fresh weight per gram. The yield amount obtained as a result of the bacterial bed cultivation using a medium in which the weight ratio of bagasse and water was 63% and 37% was 30 g per 1 kg of the medium. In addition, the yield amount obtained as a result of bacterial bed cultivation using a medium in which the weight ratio of bagasse, bran, and water was 48%, 10%, and 42% was 60 g per 1 kg of the medium.

However, as in the above-mentioned embodiment, the yield obtained as a result of the bacterial bed cultivation using the medium in which the weight ratio of bagasse, bran and water is 42%, 14% and 44% is 1 k of the medium.
It was 140 g per gram. Furthermore, the yield obtained as a result of bacterial bed cultivation using a medium having a bagasse, bran, and water weight ratio of 36%, 18%, and 46% is 100 g per 1 kg of the medium.
Met. As described above, from the experimental results shown in FIG. 2, the range of composition useful as a substitute medium for hardwood sawdust in the fungus bed cultivation using bagasse as a medium substrate is bagasse 36-4.
The weight ratio is 5%, 12 to 18% of bran, and 43 to 46% of water.

As described above, in the mushroom bed cultivation of shiitake mushrooms, bagasse which is an unused resource can be effectively utilized by selecting an appropriate composition of bagasse as a substitute base material for hardwood sawdust. Further, since the price of bagasse is as low as 1/20 that of hardwood sawdust, the production cost of shiitake mushrooms can be reduced.

[0014]

As described in detail above, according to the present invention, by using bagasse as an alternative base material for hardwood sawdust, which is a raw material for shiitake mushroom bed cultivation, effective use of bagasse, which is an unused resource, is achieved. At the same time, it is possible to provide a method for cultivating shiitake mushroom beds that solves the difficulty of obtaining sawdust and reduces the production cost of shiitake mushrooms.

[Brief description of drawings]

FIG. 1 is a process diagram showing a method for cultivating a mushroom bed of shiitake mushrooms according to an embodiment of the present invention.

FIG. 2 is an explanatory diagram showing the composition of the medium used in the experiment of the present invention and the yield of the experiment result.

[Explanation of symbols]

 1 Preparation of medium 3 Sterilization 5 Inoculation with inoculum 6 Culture 7 Flooding 8 Generation / growth

Claims (3)

[Claims] 1. A mushroom bed culture of shiitake mushrooms, in which a medium is packed in a container, sterilized and inoculated with an inoculum, and the bacterial bed is cultivated, spun, ripened, and then subjected to water infiltration to generate and grow fruiting bodies. In the method, a method for cultivating a mushroom bed of shiitake mushrooms is characterized in that the medium base material is bagasse and the nutrient additive is bran. 2. A mushroom bed culture of shiitake mushrooms, in which a medium is packed in a container, sterilized and inoculated with an inoculum, and the bacterial bed is cultivated, spun, ripened, and then subjected to waterlogging treatment to generate and grow fruiting bodies. In the method, the composition of the medium is bagasse 36-45%, bran 12-18.
%, Water 43-46% by weight ratio, Shiitake mushroom bed cultivation method characterized by the above. 3. The water content in the medium is about 65% by weight.
The method for cultivating a mushroom bed of shiitake mushroom according to claim 2, wherein