JPH0564608B2 - - Google Patents
Info
- Publication number
- JPH0564608B2 JPH0564608B2 JP8514485A JP8514485A JPH0564608B2 JP H0564608 B2 JPH0564608 B2 JP H0564608B2 JP 8514485 A JP8514485 A JP 8514485A JP 8514485 A JP8514485 A JP 8514485A JP H0564608 B2 JPH0564608 B2 JP H0564608B2
- Authority
- JP
- Japan
- Prior art keywords
- polylysine
- hydrochloride
- preservative
- test
- cosmetics
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108010039918 Polylysine Proteins 0.000 claims description 41
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 30
- 239000002537 cosmetic Substances 0.000 claims description 16
- 239000003755 preservative agent Substances 0.000 claims description 16
- 230000002335 preservative effect Effects 0.000 claims description 15
- 241000972623 Streptomyces albulus Species 0.000 claims description 5
- 239000000645 desinfectant Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 14
- 239000000203 mixture Substances 0.000 description 11
- 241000894006 Bacteria Species 0.000 description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 230000000844 anti-bacterial effect Effects 0.000 description 8
- 239000003899 bactericide agent Substances 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 8
- 239000002609 medium Substances 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 6
- 239000008213 purified water Substances 0.000 description 6
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 6
- 208000002874 Acne Vulgaris Diseases 0.000 description 5
- 206010000496 acne Diseases 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000009472 formulation Methods 0.000 description 5
- 239000003205 fragrance Substances 0.000 description 5
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 4
- 229960000686 benzalkonium chloride Drugs 0.000 description 4
- CADWTSSKOVRVJC-UHFFFAOYSA-N benzyl(dimethyl)azanium;chloride Chemical compound [Cl-].C[NH+](C)CC1=CC=CC=C1 CADWTSSKOVRVJC-UHFFFAOYSA-N 0.000 description 4
- BXWNKGSJHAJOGX-UHFFFAOYSA-N hexadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCO BXWNKGSJHAJOGX-UHFFFAOYSA-N 0.000 description 4
- 239000006210 lotion Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 3
- 229960004889 salicylic acid Drugs 0.000 description 3
- 239000003206 sterilizing agent Substances 0.000 description 3
- 229940058015 1,3-butylene glycol Drugs 0.000 description 2
- 241000222122 Candida albicans Species 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 2
- 208000001840 Dandruff Diseases 0.000 description 2
- MDNWOSOZYLHTCG-UHFFFAOYSA-N Dichlorophen Chemical compound OC1=CC=C(Cl)C=C1CC1=CC(Cl)=CC=C1O MDNWOSOZYLHTCG-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- CPELXLSAUQHCOX-UHFFFAOYSA-N Hydrogen bromide Chemical compound Br CPELXLSAUQHCOX-UHFFFAOYSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 239000004288 Sodium dehydroacetate Substances 0.000 description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 2
- -1 and if necessary Substances 0.000 description 2
- 230000000843 anti-fungal effect Effects 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000007613 bennett's agar Substances 0.000 description 2
- JFIOVJDNOJYLKP-UHFFFAOYSA-N bithionol Chemical compound OC1=C(Cl)C=C(Cl)C=C1SC1=CC(Cl)=CC(Cl)=C1O JFIOVJDNOJYLKP-UHFFFAOYSA-N 0.000 description 2
- 229960002326 bithionol Drugs 0.000 description 2
- 235000019437 butane-1,3-diol Nutrition 0.000 description 2
- 229940095731 candida albicans Drugs 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 229960000541 cetyl alcohol Drugs 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 229960003887 dichlorophen Drugs 0.000 description 2
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 description 2
- 210000004209 hair Anatomy 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 229940057995 liquid paraffin Drugs 0.000 description 2
- GLDOVTGHNKAZLK-UHFFFAOYSA-N octadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCCCO GLDOVTGHNKAZLK-UHFFFAOYSA-N 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 229940079839 sodium dehydroacetate Drugs 0.000 description 2
- 235000019259 sodium dehydroacetate Nutrition 0.000 description 2
- DSOWAKKSGYUMTF-GZOLSCHFSA-M sodium;(1e)-1-(6-methyl-2,4-dioxopyran-3-ylidene)ethanolate Chemical compound [Na+].C\C([O-])=C1/C(=O)OC(C)=CC1=O DSOWAKKSGYUMTF-GZOLSCHFSA-M 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000004334 sorbic acid Substances 0.000 description 2
- 235000010199 sorbic acid Nutrition 0.000 description 2
- 229940075582 sorbic acid Drugs 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 229920001351 ε-poly-L-lysine Polymers 0.000 description 2
- DPEYHNFHDIXMNV-UHFFFAOYSA-N (9-amino-3-bicyclo[3.3.1]nonanyl)-(4-benzyl-5-methyl-1,4-diazepan-1-yl)methanone dihydrochloride Chemical compound Cl.Cl.CC1CCN(CCN1Cc1ccccc1)C(=O)C1CC2CCCC(C1)C2N DPEYHNFHDIXMNV-UHFFFAOYSA-N 0.000 description 1
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 description 1
- BMYNFMYTOJXKLE-UHFFFAOYSA-N 3-azaniumyl-2-hydroxypropanoate Chemical compound NCC(O)C(O)=O BMYNFMYTOJXKLE-UHFFFAOYSA-N 0.000 description 1
- HIQIXEFWDLTDED-UHFFFAOYSA-N 4-hydroxy-1-piperidin-4-ylpyrrolidin-2-one Chemical compound O=C1CC(O)CN1C1CCNCC1 HIQIXEFWDLTDED-UHFFFAOYSA-N 0.000 description 1
- 241000228245 Aspergillus niger Species 0.000 description 1
- 241000186427 Cutibacterium acnes Species 0.000 description 1
- 206010015150 Erythema Diseases 0.000 description 1
- 241001646716 Escherichia coli K-12 Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 206010027627 Miliaria Diseases 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- AOMUHOFOVNGZAN-UHFFFAOYSA-N N,N-bis(2-hydroxyethyl)dodecanamide Chemical compound CCCCCCCCCCCC(=O)N(CCO)CCO AOMUHOFOVNGZAN-UHFFFAOYSA-N 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 241000283977 Oryctolagus Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 208000003251 Pruritus Diseases 0.000 description 1
- HVUMOYIDDBPOLL-XWVZOOPGSA-N Sorbitan monostearate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O HVUMOYIDDBPOLL-XWVZOOPGSA-N 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 241000191963 Staphylococcus epidermidis Species 0.000 description 1
- 235000021355 Stearic acid Nutrition 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 231100000215 acute (single dose) toxicity testing Toxicity 0.000 description 1
- 231100000403 acute toxicity Toxicity 0.000 description 1
- 230000007059 acute toxicity Effects 0.000 description 1
- 238000011047 acute toxicity test Methods 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- NNCOOIBIVIODKO-UHFFFAOYSA-N aluminum;hypochlorous acid Chemical compound [Al].ClO NNCOOIBIVIODKO-UHFFFAOYSA-N 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 239000002781 deodorant agent Substances 0.000 description 1
- PXBRQCKWGAHEHS-UHFFFAOYSA-N dichlorodifluoromethane Chemical compound FC(F)(Cl)Cl PXBRQCKWGAHEHS-UHFFFAOYSA-N 0.000 description 1
- 235000019404 dichlorodifluoromethane Nutrition 0.000 description 1
- REZZEXDLIUJMMS-UHFFFAOYSA-M dimethyldioctadecylammonium chloride Chemical compound [Cl-].CCCCCCCCCCCCCCCCCC[N+](C)(C)CCCCCCCCCCCCCCCCCC REZZEXDLIUJMMS-UHFFFAOYSA-M 0.000 description 1
- 239000004664 distearyldimethylammonium chloride (DHTDMAC) Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 231100000321 erythema Toxicity 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000011790 ferrous sulphate Substances 0.000 description 1
- 235000003891 ferrous sulphate Nutrition 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000007803 itching Effects 0.000 description 1
- 238000004898 kneading Methods 0.000 description 1
- 229940031957 lauric acid diethanolamide Drugs 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 229920000609 methyl cellulose Polymers 0.000 description 1
- 239000001923 methylcellulose Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- DVEKCXOJTLDBFE-UHFFFAOYSA-N n-dodecyl-n,n-dimethylglycinate Chemical compound CCCCCCCCCCCC[N+](C)(C)CC([O-])=O DVEKCXOJTLDBFE-UHFFFAOYSA-N 0.000 description 1
- GOQYKNQRPGWPLP-UHFFFAOYSA-N n-heptadecyl alcohol Natural products CCCCCCCCCCCCCCCCCO GOQYKNQRPGWPLP-UHFFFAOYSA-N 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- BUCIWTBCUUHRHZ-UHFFFAOYSA-K potassium;disodium;dihydrogen phosphate;hydrogen phosphate Chemical compound [Na+].[Na+].[K+].OP(O)([O-])=O.OP([O-])([O-])=O BUCIWTBCUUHRHZ-UHFFFAOYSA-K 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 229940098458 powder spray Drugs 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 239000003380 propellant Substances 0.000 description 1
- 229940055019 propionibacterium acne Drugs 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000001953 recrystallisation Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000002453 shampoo Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000001587 sorbitan monostearate Substances 0.000 description 1
- 235000011076 sorbitan monostearate Nutrition 0.000 description 1
- 229940035048 sorbitan monostearate Drugs 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 125000004079 stearyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- CYRMSUTZVYGINF-UHFFFAOYSA-N trichlorofluoromethane Chemical compound FC(Cl)(Cl)Cl CYRMSUTZVYGINF-UHFFFAOYSA-N 0.000 description 1
- 229940099259 vaseline Drugs 0.000 description 1
- 210000002268 wool Anatomy 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
- A61K8/88—Polyamides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/006—Antidandruff preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/524—Preservatives
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Dermatology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Cosmetics (AREA)
- Compositions Of Macromolecular Compounds (AREA)
Description
本発明はStreptomyces albulus No−346菌株
の生産物より分離、精製して得られるε−ポリリ
ジン（ε−poly−Ｌ−lysine）及びその塩酸塩を
防腐・殺菌剤として、配合したことを特徴とする
化粧料に関するものである。
従来より、化粧料に用いられる防腐・殺菌剤と
しては、ビチオノール、ジクロロフエン、デヒド
ロ酢酸ナトリウム、サリチル酸、ソルビン酸、ヒ
ビテン酸が汎用されている。しかし、近年、ビチ
オノール、ジクロロフエンは光接触アレルギーな
ど安全性の面で問題となつている。また、デヒド
ロ酢酸ナトリウム、サリチル酸、及びソルビン酸
は化粧料へ配合した時、経時的に変色し化粧料の
外観を著しく悪くするという問題があつた。ヒビ
テンにおいては、水系の化粧料へ配合した時、沈
澱や凝集を生じ、効果的な防腐・殺菌剤とは言え
なかつた。
かかる問題に対して鋭意研究の結果、皮膚に対
して安全性が高く、化粧料に配合しても経時的に
安定で、従来にない優れた効果を持つた水溶性の
防腐・殺菌剤を得るに到つた。
すなわち、本発明は、Streptomyces albulus
No−346菌株の生産物より分離・精製したε−ポ
リリジン及びその塩酸塩を防腐・殺菌剤として配
合した化粧料に関するものである。
本発明で使用する菌株Streptomyces albulus
No−346は、微生物の分譲機関である財団法人発
酵研究所より分譲されている（List of Cultures
（1984）、1984年（昭和59年）４月５日発行）。
本発明において使用するε−ポリリジン塩酸塩
は島、酒井らの方法〔Agric.Biol.Chem.，41，
1807−９（1977）；45，2503−８（1983）．日本農芸
化学会誌，57，221〜６（1983）．〕に基づいて製造
したもので、図１の構造式で示されるようにＬ−
リジンに１分子の塩酸と１分子の水分子を含む基
本骨格が、25〜30個重合したポリペプチドであ
る。
以下に、ε−ポリリジン塩酸塩及びε−ポリリ
ジンの製造法を示す。
〈ε−ポリリジン塩酸塩の製法〉
改良Bennett′s寒天培地上で保存した
Streptmyces albulus No−346菌株から１白金
耳を、500ml容振盪フラスコ内のε−PL培地に接
種し、30℃で好気的に24〜36時間振盪培養（前培
養）したものを種菌とする。ついで、ε−PL培
地125mlを500ml容振盪フラスコに分注し、殺菌
後、No−346株を１％接種し、30℃で好気的に36
〜48時間、培養する。
培養液は常法により、ε−ポリリジンを分離
し、ε−ポリリジン塩酸塩として精製することが
できる。すなわち、連続遠心分離器（8000rpm）
により菌体を除去し、ついでイオン交換樹脂カラ
ム（例えば、Amberlite IRC−50，米国ロー
ム・アンド・ハース社製）により吸着させる。吸
着部を0.2N酢酸で洗浄後、水洗し、さらに0.1N
塩酸を流しDragendorff試薬で陽性になる部分を
分取する。流出液を濃縮し、残渣を水に再溶解
し、エタノール：エーテル（２：１）を加えて、
白色沈澱としてε−ポリリジン塩酸塩を得る。必
要に応じ再結晶をくり返す。
改良Bennett′s 寒天培地 処方
グルコース 1.0g
肉エキス 0.1g
ペプトン 0.2g
酵母エキス 0.1g
寒天 1.5g
精製水を加えて100mlとする（PH7.5）。
ε−PL培地
グリセリン 50g
硫酸アンモニウム 10g
酵母エキス 5g
硫酸マグネシウム 0.5g
硫酸第一鉄 0.03g
硫酸亜鉛 0.04g
以上をＮ／50リン酸−カリウム−リン酸二ナト
リウム緩衝液にて１にする（PH6.8）。
〈ε−ポリリジンの製法〉
ε−ポリリジン塩酸塩を30ｇを精製水100mlに
溶解した後、Ｎ／２水酸化ナトリウム水溶液を用
いて、PH10〜11に調製し、塩素イオンが確認でき
なくなるまで透析を行なう。透析後、水を除去
し、ε−ポリリジン1.7ｇを得る。得られたε−
ポリリジンは、白色〜淡黄色の吸湿性のある粉末
で、水、アルコール等に可溶である。
なお、本発明においては、上記、ε−ポリリジ
ン、ε−ポリリジン塩酸塩の他、ε−ポリリジン
の臭化水素酸塩、ヨウ化水素酸塩等も、使用可能
で、ε−ポリリジン塩酸塩と同等の効果が認めら
れた。例えば、ε−ポリリジン臭化水素酸塩は、
ε−ポリリジンと計算量の臭化水素酸をアルコー
ルあるいは水等の溶媒中で反応させることにより
容易に得られる。
上記の如くして得られたε−ポリリジン及びそ
の塩類は、そのまま、あるいは適当な化粧料基材
に溶解、分散、混合等の処理を行ない、さらに必
要のある場合は賦形剤、乳化剤、懸濁剤、浸透
剤、その他を加えて製剤化し、本発明の防腐・殺
菌剤とすることができる。この防腐・殺菌剤は、
化粧料全般にわたつて有効に使用できるものであ
る。また、この防腐・殺菌剤は低毒性で生分解性
があるので環境汚染等の心配がない事も本発明の
利点として挙げられる。
次に本発明の防腐・殺菌剤の抗菌・抗カビ性が
いかに優れているか本発明と従来の防腐・殺菌剤
の塩化ベンザルコニウムと比較試験した結果を示
す。
試験菌
１ アスペルギルス・ニゲル（Aspergillus
niger ATCC 6275）
２ ペニシリウム・シトリヌス（Penicillum
citrinum ATCC 9845）
３ カンジダ・アルビカンス（Candida
albicans ATCC 10231）
４ エシエリヒア・コリー（Escherichia coli
K12）
５ シユウドモナス・イルギノーサ
（Psedomonas aeruginosa ATCC 9027）
６ スタフイロコツカス・アウレウス
（Staphylococcus aureus FDA 2099）
試験方法
ε−ポリリジン、ε−ポリリジン塩酸塩、及び
塩化ベンザルコニウムの0.1％水溶液（PH7.2に調
整）を作製する。この溶液を試験管に10mlずつ分
注し、この中にあらかじめ液体培地で24時間30℃
静置増殖した各試験菌をそれぞれ40μずつ接種
する。上記試験管から１mlずつを、接種30秒後、
30分後、及び２時間後、それぞれシヤーレに取り
寒天培地を用い混釈培養して生菌数を測定し、結
果を表２〜４にまとめて示す。
なお、試験管は30℃恒温器内に静置した。
The present invention is characterized by containing ε-poly-L-lysine (ε-poly-L-lysine) obtained by isolation and purification from the product of Streptomyces albulus No-346 strain and its hydrochloride as a preservative and disinfectant. It is related to cosmetics. Conventionally, bithionol, dichlorophene, sodium dehydroacetate, salicylic acid, sorbic acid, and hibitenic acid have been widely used as preservatives and disinfectants used in cosmetics. However, in recent years, bithionol and dichlorophene have become problematic in terms of safety, such as photo-contact allergy. Furthermore, when sodium dehydroacetate, salicylic acid, and sorbic acid are blended into cosmetics, there is a problem in that they discolor over time, significantly worsening the appearance of the cosmetics. When Hibiten was added to water-based cosmetics, it caused precipitation and aggregation, and could not be considered an effective preservative or bactericidal agent. As a result of intensive research into this problem, we have developed a water-soluble preservative/bactericide that is highly safe for the skin, stable over time when incorporated into cosmetics, and has unprecedented effects. I reached it. That is, the present invention provides Streptomyces albulus
This invention relates to a cosmetic containing ε-polylysine isolated and purified from the product of strain No. 346 and its hydrochloride as a preservative and bactericidal agent. Bacterial strain Streptomyces albulus used in the present invention
No.346 is distributed by the Fermentation Research Institute, a microbial distribution organization (List of Cultures
(1984), published April 5, 1984). The ε-polylysine hydrochloride used in the present invention was prepared by the method of Shima, Sakai et al. [Agric.Biol.Chem., 41 ,
1807-9 (1977); 45 , 2503-8 (1983). Journal of the Japanese Society of Agricultural Chemistry, 57 , 221-6 (1983). ], and as shown in the structural formula in Figure 1, L-
It is a polypeptide consisting of 25 to 30 polymerized basic skeletons containing lysine, one molecule of hydrochloric acid, and one molecule of water. A method for producing ε-polylysine hydrochloride and ε-polylysine is shown below. <Production method of ε-polylysine hydrochloride> Preserved on modified Bennett's agar medium
One platinum loopful of Streptmyces albulus No-346 strain was inoculated into ε-PL medium in a 500 ml shaking flask, and cultured aerobically at 30° C. for 24 to 36 hours with shaking (preculture) to serve as a seed strain. Next, 125 ml of ε-PL medium was dispensed into a 500 ml shaking flask, and after sterilization, 1% of strain No. 346 was inoculated and incubated aerobically at 30°C for 36 hours.
Incubate for ~48 hours. From the culture solution, ε-polylysine can be separated and purified as ε-polylysine hydrochloride using a conventional method. i.e. continuous centrifuge (8000rpm)
The bacterial cells are then removed using an ion exchange resin column (eg, Amberlite IRC-50, manufactured by Rohm and Haas, USA) and adsorbed. After washing the adsorption part with 0.2N acetic acid, washing with water, and then adding 0.1N
Pour in hydrochloric acid and collect the portion that becomes positive with Dragendorff's reagent. Concentrate the effluent, redissolve the residue in water and add ethanol:ether (2:1).
ε-Polylysine hydrochloride is obtained as a white precipitate. Repeat recrystallization as necessary. Improved Bennett's agar medium Recipe Glucose 1.0g Meat extract 0.1g Peptone 0.2g Yeast extract 0.1g Agar 1.5g Add purified water to make 100ml (PH7.5). ε-PL medium Glycerin 50g Ammonium sulfate 10g Yeast extract 5g Magnesium sulfate 0.5g Ferrous sulfate 0.03g Zinc sulfate 0.04g Adjust the above to 1 with N/50 phosphate-potassium-disodium phosphate buffer (PH6.8 ). <Production method of ε-polylysine> After dissolving 30g of ε-polylysine hydrochloride in 100ml of purified water, adjust the pH to 10-11 using N/2 sodium hydroxide aqueous solution, and dialysis until chloride ions can no longer be detected. Let's do it. After dialysis, water is removed to obtain 1.7 g of ε-polylysine. Obtained ε−
Polylysine is a white to pale yellow hygroscopic powder that is soluble in water, alcohol, and the like. In addition, in the present invention, in addition to the above-mentioned ε-polylysine and ε-polylysine hydrochloride, ε-polylysine hydrobromide, hydroiodide, etc. can also be used, and are equivalent to ε-polylysine hydrochloride. The effect of For example, ε-polylysine hydrobromide is
It can be easily obtained by reacting ε-polylysine with a calculated amount of hydrobromic acid in a solvent such as alcohol or water. The ε-polylysine and its salts obtained as described above may be used as they are, or may be dissolved, dispersed, mixed, etc. in an appropriate cosmetic base material, and if necessary, excipients, emulsifiers, suspending agents, etc. The preservative/sterilizing agent of the present invention can be prepared by adding a clouding agent, a penetrating agent, and others to form a formulation. This preservative/sterilizer is
It can be effectively used in all cosmetics. Another advantage of the present invention is that this preservative/sterilizer has low toxicity and is biodegradable, so there is no need to worry about environmental pollution. Next, we will show the results of a comparative test of the present invention and a conventional preservative/bactericide, benzalkonium chloride, to see how excellent the antibacterial/antifungal properties of the preservative/bactericide of the present invention are. Test bacterium 1 Aspergillus niger
niger ATCC 6275) 2 Penicillum citrinus
citrinum ATCC 9845) 3 Candida albicans (Candida
albicans ATCC 10231) 4 Escherichia coli
K12) 5 Psedomonas aeruginosa ATCC 9027 6 Staphylococcus aureus FDA 2099 Test method 0.1% aqueous solution of ε-polylysine, ε-polylysine hydrochloride, and benzalkonium chloride (PH7.2) (adjusted to). Dispense 10 ml of this solution into test tubes, and pre-fill the tubes with a liquid medium for 24 hours at 30°C.
Inoculate 40μ of each of the test bacteria grown statically. 1ml each from the above test tube, 30 seconds after inoculation,
After 30 minutes and 2 hours, each sample was placed in a petri dish and poured into an agar medium for culture, and the number of viable bacteria was measured. The results are summarized in Tables 2 to 4. In addition, the test tube was left still in a 30°C thermostat.
【表】【table】
【表】【table】
【表】【table】
【表】【table】
【表】
表２〜４の数値は１ml当りの生菌数を示す。
以上の結果より、本発明のε−ポリリジン及び
ε−ポリリジン塩酸塩は塩化ベンザルコニウムと
同等の抗菌・抗カビ性があることが明らかであ
る。また、マウスを使つた経口投与における急性
毒性試験結果を以下に示すが、ほとんど急性毒性
がなく、安全性の高い防腐・殺菌剤であることが
認められた。
ε−ポリリジン塩酸塩 LD50＞3000mg／Kg
塩化ベンザルコニウム LS50：350mg／Kg
日本白色種家兎８羽を用い、24時間閉塞貼布試
験を行ない、パツチテスト用絆創膏除去後１時間
後、24時間後、及び48時間後の判定の平均値を用
いて判定した。ε−ポリリジン及びε−ポリリジ
ン塩酸塩のいずれの場合も、紅斑及び痂皮の形成
あるいは浮腫の形成はまつたく認められず、一次
刺激性の低い防腐・殺菌剤であることが確認され
た。なお試料は、それぞれ50％水溶液のものを用
いて試験を行なつた。
次に本発明のε−ポリリジン及びε−ポリリジ
ン塩酸塩を防腐・殺菌剤として配合した化粧料に
ついて説明する。
本発明はStreptomyces albulus No−346菌株
の生産物より分離・精製したε−ポリリジン及び
ε−ポリリジン塩酸塩を防腐・殺菌剤として好ま
しくは5.0〜0.01重量％配合した化粧料に関する
ものである。化粧料の変敗を防止する防腐剤とし
て、更には皮膚表面を殺菌、消毒し、にきび、あ
せも、ただれ、ふけ、かゆみ、腋臭等を改善する
殺菌剤として化粧料に用いた場合、非常にすぐれ
たものである。さらに、防腐・殺菌剤としての効
果のほか、ε−ポリリジン及びその塩酸塩をリン
ス等に添加した場合、風合い、スタイリング、し
なやかさ等を髪に与えることができる。ε−ポリ
リジン及びその塩酸塩の化粧料への配合量が、
0.01重量％より少ない量では充分な効果が得られ
ない。また、5.0重量％を超える量では効果の増
強がないので不経済である。
次に実施例を示しながら本発明の化粧料の有効
性について説明する。なお、本発明の内容は、こ
れらに限定されるものではない。実施例及び比較
例に示す配合量の部とは重量部を示す。
実施例１ アクネ化粧水
処方 配合量
エタノール 8.0部
１，３−ブチレングリコール 7.0
クエン酸 0.03
クエン酸ナトリウム 0.15
ε−ポリリジン塩酸塩 0.5
サリチル酸 0.1
コロイドイオウ 0.1
香料 適量
精製水 83.0
製造方法：各成分を均一に溶解混合する。
比較例 １
実施例１からε−ポリリジン塩酸塩を除いた処
方で、化粧水を調製した。
実施例１と比較例１の化粧水について前記の抗
菌力試験方法で、１ピロピオバクテリウム・アク
ネス（Propionibacterium acnes）と２スタフイ
ロコツカス・エピダーミデイス
（Staphylococcus epidermidis）の２種のアクネ
菌に対する抗菌力試験を行なつた。
試験結果を表５にまとめて示す。なお、１の菌
については、GAM半流動培地に37℃、24時間培
養した液を試験菌液とし、生菌数測定はGAM寒
天にて37℃、72時間嫌気性培養下（スチール・ウ
ール法）で培養した。[Table] The values in Tables 2 to 4 indicate the number of viable bacteria per ml.
From the above results, it is clear that ε-polylysine and ε-polylysine hydrochloride of the present invention have antibacterial and antifungal properties equivalent to benzalkonium chloride. In addition, the results of an acute toxicity test using mice for oral administration are shown below, and it was found that it has almost no acute toxicity and is a highly safe preservative/sterilizer. ε-Polylysine hydrochloride LD 50 >3000mg/Kg Benzalkonium chloride LS 50 : 350mg/Kg A 24-hour occlusive patch test was conducted using 8 Japanese white rabbits, and 1 hour after removal of the patch test adhesive, 24-hour occlusive patch test was performed. Judgment was made using the average value of the judgments after hours and 48 hours. In both cases of ε-polylysine and ε-polylysine hydrochloride, no erythema, crusting, or edema formation was observed, confirming that they are antiseptic/sterilizing agents with low primary irritation. The test was conducted using a 50% aqueous solution of each sample. Next, a cosmetic composition containing ε-polylysine and ε-polylysine hydrochloride of the present invention as a preservative/sterilizing agent will be explained. The present invention relates to a cosmetic containing preferably 5.0 to 0.01% by weight of ε-polylysine and ε-polylysine hydrochloride isolated and purified from the product of Streptomyces albulus No-346 strain as a preservative and bactericidal agent. It is extremely effective when used in cosmetics as a preservative to prevent cosmetics from spoiling, and also as a bactericide to sterilize and disinfect the skin surface and improve acne, heat rash, sores, dandruff, itching, armpit odor, etc. It is something that Furthermore, in addition to its effects as a preservative and bactericidal agent, when ε-polylysine and its hydrochloride are added to a rinse or the like, it can impart texture, styling, suppleness, etc. to the hair. The amount of ε-polylysine and its hydrochloride added to cosmetics is
If the amount is less than 0.01% by weight, sufficient effects cannot be obtained. Moreover, if the amount exceeds 5.0% by weight, the effect will not be enhanced and it will be uneconomical. Next, the effectiveness of the cosmetics of the present invention will be explained with reference to Examples. Note that the content of the present invention is not limited to these. The amounts shown in Examples and Comparative Examples refer to parts by weight. Example 1 Acne lotion Prescription Amount Ethanol 8.0 parts 1,3-butylene glycol 7.0 Citric acid 0.03 Sodium citrate 0.15 ε-Polylysine hydrochloride 0.5 Salicylic acid 0.1 Colloidal sulfur 0.1 Fragrance Appropriate amount Purified water 83.0 Manufacturing method: Mix each component uniformly Dissolve and mix. Comparative Example 1 A lotion was prepared using the formulation of Example 1 except that ε-polylysine hydrochloride was removed. The lotions of Example 1 and Comparative Example 1 were tested for antibacterial activity against two types of acne bacteria: 1. Propionibacterium acnes and 2. Staphylococcus epidermidis. A force test was conducted. The test results are summarized in Table 5. Regarding bacteria 1, the test bacteria was cultured in GAM semi-solid medium at 37℃ for 24 hours, and the number of viable bacteria was measured under anaerobic culture in GAM agar at 37℃ for 72 hours (steel wool method). ) was cultured.
【表】
上記の試験結果より明らかなように、ε−ポリ
リジン塩酸塩配合の化粧水は、アクネ菌に対して
強い抗菌作用が認められた。
実施例２ アクネクリーム
処方 配合量
Ａ ステアリン酸 4.0部
セチルアルコール 3.0
ステアリルアルコール 1.0
流動パラフイン 6.5
ワセリン 10.0
ソルビタンモノステアレート 1.5
ポリオキシエチレンモノステアレート 3.0
Ｂ １，３−ブチレングリコール 5.0
水酸化カリウム 0.1
ε−ポリリジン 0.3
コロイドイオウ 2.0
精製水 63.6
Ｃ 香料 適量
製造方法：油相成分Ａ）及び水相成分Ｂ）をそれ
ぞれ70〜75℃に加熱溶解した後、成分Ａに成分
Ｂを加えて乳化し、冷却途上で成分Ｃ）を加え
混合し、30℃まで冷却し製品とする。
実施例３ ふけ取りシヤンプー
処方 配合量
Ａ メチルセルロース 0.5部
精製水 77.0
アルキゾ硫酸トリエタノールアミン 18.0
Ｂ ラウリン酸ジエタノールアミド 3.0
香料 適量
Ｃ ε−ポリリジン塩酸塩 0.5
製造方法：成分Ａ）を70〜75℃で加熱溶解した
後、成分Ｂ）を加え、冷却途中で成分Ｃ）を加
えて30℃まで冷却し製品とする。
実施例４ デオドラントパウダースプレー
処方 配合量
Ａ タルク 3.0部
アルミニウムヒドロキシクロリド 3.0
イソプロピルミリステート 1.0
フエニルメチルポリシロキサン 2.0
ε−ポリリジン塩酸塩 0.1
香料 適量
Ｂ フロン11 45.0
フロン12 45.0
製造方法：成分Ａ）を均一混練りし、容器に充填
後、噴射剤Ｂ）を充填し製品とする。
実施例５ リンス
処方 配合量
Ａ ジステアリルジメチルアンモニウムクロリド
4.5
グリセリンモノステアレート 3.5
流動パラフイン 1.5
セチルアルコール 2.0
ラウリルジメチルアミノ酢酸ベタイン 1.5
Ｂ 精製水 82.0
ステアリルトリメチツルアンモニウムクロリ
ド 4.5
ε−ポリリジン塩酸塩 0.5
Ｃ 香料 適量
製造方法：成分Ａ）及び成分Ｂ）をそれぞれ70〜
75℃に加熱溶解した後、混合し、冷却途中で成
分Ｃ）を加えて30℃まで冷却し製品とする。
比較例５ リンス
実施例５からε−ポリリジン塩酸塩を除いた処
方で、リンスを調製した。
実施例５と比較例５のリンスについて、25〜50
才の一般女性40名を対象に使用テストを行ない、
ダブルブラインド法により、風合を中心にアンケ
ート調査を行なつた。１カ月間使用した結果を表
６にまとめて示した。表６でも明らかなように、
髪の風合い、しなやかさ、スタイリング等に良好
な結果が得られた。[Table] As is clear from the above test results, the lotion containing ε-polylysine hydrochloride was found to have a strong antibacterial effect against acne bacteria. Example 2 Acne cream formulation Amount A Stearic acid 4.0 parts Cetyl alcohol 3.0 Stearyl alcohol 1.0 Liquid paraffin 6.5 Vaseline 10.0 Sorbitan monostearate 1.5 Polyoxyethylene monostearate 3.0 B 1,3-butylene glycol 5.0 Potassium hydroxide 0.1 ε- Polylysine 0.3 Colloidal sulfur 2.0 Purified water 63.6 C Fragrance Appropriate amount Production method: After heating and dissolving oil phase component A) and water phase component B) at 70 to 75°C, add component B to component A and emulsify, while cooling. Add component C), mix, and cool to 30°C to form a product. Example 3 Dandruff shampoo formulation Amount A Methylcellulose 0.5 parts Purified water 77.0 Alkizosulfate triethanolamine 18.0 B Lauric acid diethanolamide 3.0 Fragrance appropriate amount C ε-Polylysine hydrochloride 0.5 Manufacturing method: Heat component A) at 70-75°C After dissolving, add component B), add component C) during cooling, and cool to 30°C to form a product. Example 4 Deodorant powder spray formulation Amount A Talc 3.0 parts Aluminum hydroxychloride 3.0 Isopropyl myristate 1.0 Phenylmethylpolysiloxane 2.0 ε-polylysine hydrochloride 0.1 Fragrance Appropriate amount B Freon 11 45.0 Freon 12 45.0 Manufacturing method: Component A) was uniformly mixed After kneading and filling a container, the product is filled with propellant B). Example 5 Rinse prescription Blend amount A Distearyldimethylammonium chloride
4.5 Glycerin monostearate 3.5 Liquid paraffin 1.5 Cetyl alcohol 2.0 Betaine lauryldimethylaminoacetate 1.5 B Purified water 82.0 Stearyl trimethytulammonium chloride 4.5 ε-Polylysine hydrochloride 0.5 C Fragrance Proper amount Production method: 70% each of component A) and component B) ~
After heating and dissolving at 75°C, mix, add component C) during cooling, and cool to 30°C to form a product. Comparative Example 5 Rinse A rinse was prepared using the recipe of Example 5 except that ε-polylysine hydrochloride was removed. For rinsing in Example 5 and Comparative Example 5, 25 to 50
We conducted a usage test on 40 ordinary women of the same age.
A questionnaire survey was conducted using a double-blind method, focusing on texture. Table 6 summarizes the results of using the product for one month. As is clear from Table 6,
Good results were obtained in hair texture, flexibility, styling, etc.
Claims (1)
物より分離、精製したε−ポリリジン及びその塩
酸塩を防腐・殺菌剤として配合したことを特徴と
する化粧料。1. A cosmetic containing ε-polylysine isolated and purified from the product of Streptomyces albulus No-346 strain and its hydrochloride as a preservative and disinfectant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8514485A JPS61243010A (en) | 1985-04-20 | 1985-04-20 | Cosmetic containing epsilon-polylysine and hydrochloride thereof as preservative and germicide incorporated therein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8514485A JPS61243010A (en) | 1985-04-20 | 1985-04-20 | Cosmetic containing epsilon-polylysine and hydrochloride thereof as preservative and germicide incorporated therein |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61243010A JPS61243010A (en) | 1986-10-29 |
| JPH0564608B2 true JPH0564608B2 (en) | 1993-09-16 |
Family
ID=13850463
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP8514485A Granted JPS61243010A (en) | 1985-04-20 | 1985-04-20 | Cosmetic containing epsilon-polylysine and hydrochloride thereof as preservative and germicide incorporated therein |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61243010A (en) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0220295A (en) * | 1988-07-08 | 1990-01-23 | Chisso Corp | Production of free epsilon-polylysine |
| DE69331597T2 (en) * | 1992-11-25 | 2002-09-12 | Chisso Corp | Methods and use of poly-L-lysine as an enzyme protection agent |
| JP2002012536A (en) * | 2000-06-27 | 2002-01-15 | Lion Corp | Composition for oral cavity |
| JP2004026743A (en) * | 2002-06-27 | 2004-01-29 | Chisso Corp | Polylysine preparation and cosmetic composition containing the same |
| JP2004035461A (en) * | 2002-07-03 | 2004-02-05 | Chisso Corp | Odorproof deodorant |
| JP2004067586A (en) * | 2002-08-06 | 2004-03-04 | Chisso Corp | Antimicrobial agent composition and antimicrobial sheet-like article using the same |
| JP2004182639A (en) * | 2002-12-03 | 2004-07-02 | Shiseido Co Ltd | Skin care preparation composition for external use |
| JP4461727B2 (en) * | 2003-07-18 | 2010-05-12 | チッソ株式会社 | External preparation composition |
| JP4947034B2 (en) * | 2008-10-30 | 2012-06-06 | Ｊｎｃ株式会社 | Polylysine preparation and cosmetic composition containing the same |
| DE102010043073A1 (en) * | 2010-10-28 | 2012-05-03 | Henkel Ag & Co. Kgaa | Active substance combination and hair treatment agent for dandruff IV |
| DE102010043074A1 (en) * | 2010-10-28 | 2012-05-03 | Henkel Ag & Co. Kgaa | Active substance combination and hair treatment agent for dandruff III |
| DE102010043075A1 (en) * | 2010-10-28 | 2012-05-03 | Henkel Ag & Co. Kgaa | Active substance combination and hair treatment agent for dandruff I |
| DE102010043076A1 (en) * | 2010-10-28 | 2012-05-03 | Henkel Ag & Co. Kgaa | Active substance combination and hair treatment agent for dandruff II |
| DE102010062662A1 (en) * | 2010-12-08 | 2012-06-14 | Henkel Ag & Co. Kgaa | Active ingredient combination and hair treatment agent |
| DE102011079538A1 (en) * | 2011-07-21 | 2013-01-24 | Henkel Ag & Co. Kgaa | Alcoholic hair treatment agent with poly-L-lysine |
| DE102011079540A1 (en) * | 2011-07-21 | 2013-01-24 | Henkel Ag & Co. Kgaa | Improved active ingredient combination and hair treatment agent for dandruff I |
| DE102011079539A1 (en) * | 2011-07-21 | 2013-01-24 | Henkel Ag & Co. Kgaa | Performance Enhanced Drug Combination and Hair Treatments for Dandruff II |
| CN108498401A (en) * | 2018-05-25 | 2018-09-07 | 钱兴 | A kind of preparation method of mouthwash |
-
1985
- 1985-04-20 JP JP8514485A patent/JPS61243010A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS61243010A (en) | 1986-10-29 |
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