JPH0562245U - Blood filter - Google Patents

Abstract

(57) [Summary] [Purpose] Thaw blood can be easily removed in a sealed state. [Structure] Sealed cleaning container 2 in which thawed blood is sent and stored
The washing liquid is ejected from the hole 7 at the tip of the injection pipes 3a and 3b to mix the thawed blood and the washing liquid. Hollow fiber 9 with a mixture of thawed blood and a washing solution having both end openings connected to drain holes 11
The antifreezing liquid is removed by filtering with a filter unit 4 having a bundle of a. A collection tube 5 for the washed blood only, with a tip opening near the bottom of the washing container 2.
To collect.

Description

[Detailed description of the device]

[0001]

[Industrial applications]

 The present invention relates to a hemofilter for washing stored blood, and particularly to improvement of washing efficiency.

[0002]

[Prior Art]

 As the most effective means for preventing side effects and infection accidents due to blood transfusion, a method is widely adopted in which blood of a patient, that is, autologous blood is collected and frozen and stored prior to surgery, and then used. In addition, the cryopreservation method is also used for the preservation of so-called blood products even if they are not autologous blood. Cryopreservation of blood is performed by dividing the blood into two components, a red blood cell component and a plasma component, and a red blood cell component, for example, to prevent destruction of blood cells when water holding red blood cells freezes. Anti-freezing liquid consisting of rust is added. In order to use cryopreserved blood, it is necessary to remove the antifreezing liquid from the thawed blood obtained by thawing the preserved blood.

Conventionally, a reversible collection method and a centrifugation method have been adopted as a method for removing the antifreezing liquid. In the reversible method, the solution of glucose, fructose and salt is added to the thawed blood in the bag and stirred, and the antifreeze solution is floated above the upper layer of the mixture to remove it, and the concentrated and sedimented blood is removed. obtain. This operation is repeated to obtain blood from which the antifreezing liquid has been removed. In the centrifugation method, saline is added to the thawed blood as a washing solution to separate erythrocytes by centrifugal separation. During this separation, the concentration of saline solution is gradually lowered so that the destruction of red blood cells, so-called hemolysis, is not performed, and this operation is repeated in several steps.

Further, there is a method of removing the antifreezing liquid from the thawed blood by flowing a washing liquid outside the hollow fibers while pumping the thawed blood into a plurality of porous hollow fibers by a pump, for example. Is disclosed in.

[0005]

[Problems to be solved by the device]

 The above-mentioned reversible collection method and centrifugation method are so-called batch methods, and their operations are complicated. Therefore, only trained technicians can perform the operations and the operation time is long.

Further, since the antifreezing liquid is removed by repeatedly adding an aqueous solution of glucose or the like or a cleaning liquid, there is a risk that blood may be contaminated with bacteria or the like in the process of operation.

[0007] Furthermore, the centrifugal separation method and the method of pumping thawed blood to the inside of the hollow fiber are unavoidable because hemolysis is unavoidable because blood is subjected to considerable force. In order to remove the anti-freezing liquid to a transfusing level, it is necessary to dilute it with a cleaning liquid while pumping it in the hollow fiber several times to circulate it, which exerts a great pressure on the blood. Unlike fresh blood, this large pressure has a disadvantage in that stored blood with weak cell vitality causes decisive hemolysis.

The present invention has been made in order to solve the above disadvantages, and an object thereof is to obtain a hemofilter capable of easily removing thawed blood in a sealed state.

[0009]

[Means for Solving the Problems]

 The blood filtering device according to the present invention has a washing container, an injection pipe, a filter portion, and a collection pipe, and the washing container mixes thawed blood and washing liquid and stores them. The tubes are attached and sealed, and the infusion tube sends thawed blood and washing solution into the washing container respectively, and has a plurality of holes on the tip and side walls, and the filter section filters the thawed blood. , A U-shaped bundle of hollow fibers housed in a washing container, and a connecting portion for connecting both end openings of the hollow fibers to drain holes, and a collection tube for collecting washed blood. The tip opening is located at the bottom of the cleaning container from the tip of the injection tube.

Further, it is preferable that a plurality of injection pipes are provided, and the middle portion or the tip end portion of each injection pipe is connected by a connecting member.

[0011]

[Action]

 In this invention, the thawed blood and the washing liquid are mixed by ejecting the washing liquid from the hole at the tip of the injection tube into the sealed washing container in which the thawed blood is sent and stored. The antifreeze liquid is removed by filtering the mixed solution of thawed blood and washing liquid through a filter section having a bundle of hollow fibers whose both openings are connected to drain holes, and the blood is sedimented by washing. Only the washed blood is collected by the collection tube whose tip opening is near the bottom of the washing container.

[0012]

【Example】

 1 and 2 show an embodiment of the present invention, FIG. 1 is a front sectional view, and FIG. 2 is a side sectional view. As shown in FIGS. 1 and 2, the blood filtering device 1 has a washing container 2, a plurality of injection tubes 3, a filter section 4, a recovery tube 5 and an air introduction tube 13. The cleaning container 2 is a container for mixing thawed blood and a cleaning liquid, and is made of a synthetic resin molded product such as polyethylene, polypropylene, or vinyl chloride. Are attached, and the bottom surface is inclined and the portion where the recovery pipe 5 is attached is deeper than the other portions. A pressure sensor mounting portion 6 is provided on the side wall of the cleaning container 2. The injection pipes 3a and 3b serve to deliver the thawed blood and the washing liquid into the washing container 2, and have a plurality of holes 7 at the tip and the side wall of the tip portion. The intermediate portions of the injection pipes 3a and 3b are connected by a connecting rod 8. The filter unit 4 filters thawed blood, and as shown in the side sectional view of FIG. 2, the U-shaped bundle of hollow fibers 9 housed in the washing container 2 and both ends of the hollow fibers 9 are opened. It has a connecting portion 12 which is attached in a state as it is with resin or the like, and which connects both end openings of the hollow fiber 9 to the drain hole 11 of the discharge pipe 10. The U-shaped bent portion of the bundle of hollow fibers 9 is positioned by the connecting rod 8 that connects the injection pipes 3a and 3b.

FIG. 3 is a layout view showing a usage state of the hemofiltration device 1. When using the hemofiltration device 1, a plurality of washing solution bags 20a, 20b, 20c, a blood storage bag 21, and a blood storage bag 22 are connected to a reversible liquid supply pump 24 via valves 23a to 23e, respectively. deep. A high-concentration cleaning liquid, for example, an aqueous solution of glucose, fructose, and salt is sealed in the cleaning liquid bag 20a in a fixed amount according to the amount of the antifreezing liquid in the stored blood, and the cleaning liquid bag 20b has a medium-concentration cleaning liquid. However, a certain amount of low-concentration cleaning liquid is sealed in the cleaning liquid back 20c. A fixed amount of stored blood is thawed and sealed in the blood storage bag 21.

In this state, the blood filter device 1 which has been sterilized in advance is housed in a protective case 26 mounted on a vibration generator 25, and the injection pipes 3a and 3b and the recovery pipe 5 are inserted via valves 27 and 28, respectively. To the liquid supply pump 24 and the drain pipe 10 to the drain tank 30. Then, the pressure sensor 32 connected to the pump controller 31 is fixed to the pressure sensor mounting portion 6 of the cleaning container 2. Further, a sterilization filter 34 is connected to the air introduction pipe 13 via a valve 33.

After that, a cleaning process is started. First, with the valve 33 of the air introduction pipe 13 and the valve 27 connected to the injection pipes 3a and 3b open, the valve 23d of the blood storage bag 21 is opened, and the liquid supply pump 24 is rotated in the forward direction. Then, the thawed blood containing the antifreezing liquid in the blood storage bag 21 is sent to the washing container 2 through the injection pipes 3a and 3b. When all the thawed blood in the blood storage bag 21 has been sent to the washing container 2, the liquid supply pump 24 is temporarily stopped and the valve 23d and the valve 33 of the air introduction pipe 13 are closed.

After that, the valve 23a of the cleaning liquid bag 20a is opened and the liquid feed pump 24 is rotated in the normal direction to continuously supply the cleaning liquid having the highest concentration stored in the cleaning bag 20a to the cleaning container 2 through the injection pipes 3a and 3b. And send by pressure. When the cleaning liquid is pressure-fed, the pressure in the cleaning container 2 is detected by the pressure sensor 32, and the pump control unit 31 controls the rotation speed of the liquid supply pump 24 so that the internal pressure of the cleaning container 2 does not exceed a predetermined constant value. Are in control. At the same time, the vibration generator 25 is operated to apply vibration to the cleaning container 2. The cleaning liquid sent to the cleaning container 2 is ejected from the tips of the injection pipes 3a and 3b and the holes 7 provided in the side wall of the distal end, and the cleaning container 2 is vibrated, so that the thawed blood and the cleaning liquid are surely mixed and stirred. be able to.

The thawed blood that is mixed and stirred with this washing liquid is filtered by the wall membrane of the hollow fiber 9. When the thawed blood mixed and stirred with this washing liquid is filtered, the antifreezing liquid and the washing liquid become a supernatant in the washing container 2 and the blood sediments and separates into layers, and only a part of the sedimented blood is hollow. It contacts the U-shaped bend of the thread 9. On the other hand, the pressure loss of the hollow fiber 9 is the largest in the U-shaped bent portion farthest from the end opening, and is the smallest in the vicinity of the connecting portion 12. Therefore, most of the filtration by the wall membrane of the hollow fiber 9 is performed in the vicinity of the connecting portion 12, and only the antifreezing liquid and the cleaning liquid are sent to the inside of the hollow fiber 9. The antifreezing liquid and the cleaning liquid sent to the inside of the hollow fiber 9 are recovered from the end opening to the drain tank 30 through the discharge pipe 10.

As described above, when the thawed blood is filtered, only the antifreezing liquid and the cleaning liquid are pumped in the hollow fiber 9 and the blood is not pumped in the hollow fiber 9. Therefore, extra physical force is applied to the blood. It can be filtered without.

When the washing liquid is continuously sent to the washing container 2 to filter the thawed blood, the concentration of the antifreezing liquid in the thawed blood in the washing container 2 gradually decreases. Then, when the cleaning liquid stored in the cleaning liquid bag 20a is completely sent, the valve 23a of the cleaning liquid bag 20a is closed and the valve 23b of the cleaning liquid bag 20b is opened to send the cleaning liquid having a medium concentration to the cleaning container 2 to filter the thawed blood. .. When the medium-concentration cleaning liquid has been sent, the valve 23b of the cleaning liquid bag 20b is closed, the valve 23c of the cleaning liquid bag 20c is opened, and the low-concentration cleaning liquid is sent to the cleaning container 2 to filter the thawed blood. As described above, the cleaning liquid having different concentrations depending on the concentration of the antifreezing liquid in the thawed blood is switched to the cleaning container 2 by switching a predetermined amount, and is sent to the cleaning container 2 for filtration. Since no force is applied, hemolysis of blood can be prevented. Further, since the inside of the cleaning container 2 does not come into contact with the outside air when switching to the cleaning liquid having a different concentration, it is possible to prevent the blood in the cleaning container 2 from being contaminated by various bacteria.

When the fixed amount of the low-concentration cleaning liquid is completely fed and the concentration of the antifreezing liquid in the thawed blood is sufficiently reduced, the liquid supply pump 24 is stopped. After that, the valve 23e of the blood storage bag 22 is opened, the valve 27 connected to the injection pipes 3a and 3b is closed, the valve 28 connected to the recovery pipe 5 and the valve 33 of the air introduction pipe 13 are opened, and the liquid supply pump. Rotate 24 in the opposite direction. By the reverse rotation of the liquid supply pump 24, the cleaned blood solution in the cleaning container 2 is collected in the blood storage bag 22. When a certain amount of the washed blood is collected, the valve 23e, the valve 28, and the valve 33 are closed, the liquid supply pump 24 is stopped, and the washing operation is completed.

When collecting the washed blood, the collecting pipe 5 collects the blood from the bottom portion of the washing container 2, so that only the washed blood settled in the lower part of the washing container 2 can be collected. it can. As a result of washing the thawed blood in this manner, the washing time was 1/2 or less as compared with the conventional reversible collection method and centrifugation method.

In the above embodiment, the case where the injection pipes 3a and 3b and the recovery pipe 5 are connected to the single liquid feed pump 24 through the valves 27 and 28 has been described, but as shown in FIG. The collection tube 5 and the blood storage bag 22 may be connected by another liquid supply pump 35. In this case, the flow path for sending the thawed blood or the washing liquid and the flow path for the washed blood can be separated, and therefore the washing liquid can be prevented from being mixed with the blood collected in the blood storage bag 22. ..

[0023]

[Effect of the device]

 As described above, the present invention is designed such that the cleaning solution is jetted from the hole at the tip of the injection tube into the sealed cleaning container in which the thawed blood is sent and stored to mix the thawed blood with the cleaning solution. The cleaning liquid and the cleaning liquid can be surely mixed and stirred.

Further, by filtering the mixed solution of the thawed blood and the washing solution through the filter section having a bundle of hollow fibers whose both openings are connected to the drain holes, only the antifreezing liquid and the washing solution are pumped into the hollow fibers. Since blood is not pumped into the hollow fibers, it can be filtered without applying extra physical force to blood, and hemolysis of blood can be prevented.

Furthermore, since the inside of the cleaning container does not come into contact with the outside air when switching to the cleaning liquid having a different concentration, it is possible to prevent the blood in the cleaning container from being contaminated by various bacteria.

Further, since the sedimented blood is collected by the collection tube, only the washed blood can be collected.

[Brief description of drawings]

FIG. 1 is a front sectional view showing an embodiment of the present invention.

FIG. 2 is a side sectional view of the above embodiment.

FIG. 3 is a layout view showing a usage state of the embodiment.

FIG. 4 is a layout view showing another usage state of the embodiment.

[Explanation of symbols]

 1 Blood Filtration Device 2 Washing Bags 3a, 3b Injection Tube 4 Filter Section 6 Pressure Sensor Attachment Section 8 Connecting Rod 9 Hollow Fiber

Claims (2)

[Scope of utility model registration request] 1. A blood filtering device having a washing container, an injection pipe, a filter portion, and a collection pipe, wherein the washing container mixes thawed blood and a washing liquid, and stores the mixture. The tube is attached and sealed, the injection tube sends thawed blood and the washing solution into the washing container, respectively, has a plurality of holes at the tip and the side wall, and the filter section filters the thawed blood. It has a bundle of hollow fibers that are shaped like a letter and are stored in the washing container, and a connecting portion that connects both end openings of the hollow fibers to the drain holes, and the recovery tube is for collecting the washed blood, and the tip is A blood filtering device, wherein the opening is located at the bottom of the washing container from the tip of the injection tube. 2. The blood filtering device according to claim 1, wherein a plurality of injection pipes are provided, and the middle portion or the tip end portion of each injection pipe is connected by a connecting member.