JPH0555816B2 - - Google Patents
Info
- Publication number
- JPH0555816B2 JPH0555816B2 JP62228730A JP22873087A JPH0555816B2 JP H0555816 B2 JPH0555816 B2 JP H0555816B2 JP 62228730 A JP62228730 A JP 62228730A JP 22873087 A JP22873087 A JP 22873087A JP H0555816 B2 JPH0555816 B2 JP H0555816B2
- Authority
- JP
- Japan
- Prior art keywords
- glucose
- enzyme
- electrode
- enzyme electrode
- sucrose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 102000004190 Enzymes Human genes 0.000 claims description 68
- 108090000790 Enzymes Proteins 0.000 claims description 68
- 239000000126 substance Substances 0.000 claims description 31
- 238000010790 dilution Methods 0.000 claims description 24
- 239000012895 dilution Substances 0.000 claims description 24
- 238000005259 measurement Methods 0.000 claims description 24
- 229940088598 enzyme Drugs 0.000 description 64
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 55
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 51
- 239000008103 glucose Substances 0.000 description 47
- 229930006000 Sucrose Natural products 0.000 description 28
- 239000005720 sucrose Substances 0.000 description 28
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 27
- 239000000523 sample Substances 0.000 description 14
- 238000011088 calibration curve Methods 0.000 description 11
- 108010015776 Glucose oxidase Proteins 0.000 description 8
- 239000004366 Glucose oxidase Substances 0.000 description 8
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 8
- 229940116332 glucose oxidase Drugs 0.000 description 8
- 235000019420 glucose oxidase Nutrition 0.000 description 8
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 7
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 7
- 108010051210 beta-Fructofuranosidase Proteins 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 239000001573 invertase Substances 0.000 description 5
- 235000011073 invertase Nutrition 0.000 description 5
- 108010093096 Immobilized Enzymes Proteins 0.000 description 4
- 239000013543 active substance Substances 0.000 description 4
- 102000020006 aldose 1-epimerase Human genes 0.000 description 4
- 108091022872 aldose 1-epimerase Proteins 0.000 description 4
- WQZGKKKJIJFFOK-DVKNGEFBSA-N alpha-D-glucose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-DVKNGEFBSA-N 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 4
- 238000006911 enzymatic reaction Methods 0.000 description 4
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 3
- 239000004809 Teflon Substances 0.000 description 3
- 229920006362 Teflon® Polymers 0.000 description 3
- 239000012491 analyte Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 229930091371 Fructose Natural products 0.000 description 2
- 239000005715 Fructose Substances 0.000 description 2
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 2
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000004811 liquid chromatography Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 229920006395 saturated elastomer Polymers 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 108010053835 Catalase Proteins 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 241000235646 Cyberlindnera jadinii Species 0.000 description 1
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 description 1
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 1
- 102100022624 Glucoamylase Human genes 0.000 description 1
- RGHNJXZEOKUKBD-SQOUGZDYSA-N Gluconic acid Natural products OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 description 1
- 241000235527 Rhizopus Species 0.000 description 1
- BZHJMEDXRYGGRV-UHFFFAOYSA-N Vinyl chloride Chemical compound ClC=C BZHJMEDXRYGGRV-UHFFFAOYSA-N 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 238000002144 chemical decomposition reaction Methods 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 239000012468 concentrated sample Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000002848 electrochemical method Methods 0.000 description 1
- 230000032050 esterification Effects 0.000 description 1
- 238000005886 esterification reaction Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000004401 flow injection analysis Methods 0.000 description 1
- 239000000174 gluconic acid Substances 0.000 description 1
- 235000012208 gluconic acid Nutrition 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 150000003445 sucroses Chemical class 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
- C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Emergency Medicine (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62228730A JPS6469944A (en) | 1987-09-11 | 1987-09-11 | Measuring apparatus using enzyme electrode |
| DE19883851025 DE3851025T2 (de) | 1987-09-11 | 1988-09-12 | Verfahren und Vorrichtung zur Bestimmung von zwei verschiedenen Substanzen in einer Probe unter Verwendung von Enzymelektroden. |
| EP19880114860 EP0310824B1 (en) | 1987-09-11 | 1988-09-12 | Method and apparatus for the determination of two different substances in a sample using enzyme electrodes |
| US07/727,341 US5225321A (en) | 1987-09-11 | 1991-07-08 | Measuring apparatus using enzyme electrodes and the method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62228730A JPS6469944A (en) | 1987-09-11 | 1987-09-11 | Measuring apparatus using enzyme electrode |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6469944A JPS6469944A (en) | 1989-03-15 |
| JPH0555816B2 true JPH0555816B2 (US07696358-20100413-C00002.png) | 1993-08-18 |
Family
ID=16880912
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62228730A Granted JPS6469944A (en) | 1987-09-11 | 1987-09-11 | Measuring apparatus using enzyme electrode |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP0310824B1 (US07696358-20100413-C00002.png) |
| JP (1) | JPS6469944A (US07696358-20100413-C00002.png) |
| DE (1) | DE3851025T2 (US07696358-20100413-C00002.png) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2259771A (en) * | 1991-09-20 | 1993-03-24 | Tihomir Paul Obrenovitch | Electrochemical throughflow enzyme biosensor |
| US5510244A (en) * | 1992-01-30 | 1996-04-23 | Kanzaki Paper Manufacturing Co., Ltd. | Apparatus and method for assaying optical isomers |
| US5306413A (en) * | 1992-03-31 | 1994-04-26 | Kanzaki Paper Manufacturing Co., Ltd. | Assay apparatus and assay method |
| DE69720391T2 (de) * | 1996-12-20 | 2004-02-12 | Matsushita Electric Industrial Co., Ltd., Kadoma | Cholesterinsensor und Verfahren zu seiner Herstellung |
| JP4934817B2 (ja) * | 2006-03-31 | 2012-05-23 | 国立大学法人 香川大学 | マイクロフロー型バイオセンサおよび希少糖の検出または定量への使用 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6193948A (ja) * | 1984-10-13 | 1986-05-12 | Shimadzu Corp | スクロ−スセンサ |
-
1987
- 1987-09-11 JP JP62228730A patent/JPS6469944A/ja active Granted
-
1988
- 1988-09-12 EP EP19880114860 patent/EP0310824B1/en not_active Expired - Lifetime
- 1988-09-12 DE DE19883851025 patent/DE3851025T2/de not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6469944A (en) | 1989-03-15 |
| EP0310824B1 (en) | 1994-08-10 |
| EP0310824A2 (en) | 1989-04-12 |
| DE3851025D1 (de) | 1994-09-15 |
| EP0310824A3 (en) | 1989-11-02 |
| DE3851025T2 (de) | 1994-12-01 |
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| JPS6328264B2 (US07696358-20100413-C00002.png) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |