JPH0530957A - Apparatus for culturing - Google Patents

Apparatus for culturing

Info

Publication number
JPH0530957A
JPH0530957A JP19017391A JP19017391A JPH0530957A JP H0530957 A JPH0530957 A JP H0530957A JP 19017391 A JP19017391 A JP 19017391A JP 19017391 A JP19017391 A JP 19017391A JP H0530957 A JPH0530957 A JP H0530957A
Authority
JP
Japan
Prior art keywords
culture
gas
tank
culture solution
liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP19017391A
Other languages
Japanese (ja)
Inventor
Akiko Miya
晶子 宮
Itaru Umeda
到 梅田
Taisuke Toya
泰典 遠矢
Tadashi Adachi
正 足立
Etsuko Takizawa
悦子 滝沢
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ebara Corp
Ebara Research Co Ltd
Original Assignee
Ebara Corp
Ebara Research Co Ltd
Ebara Infilco Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ebara Corp, Ebara Research Co Ltd, Ebara Infilco Co Ltd filed Critical Ebara Corp
Priority to JP19017391A priority Critical patent/JPH0530957A/en
Publication of JPH0530957A publication Critical patent/JPH0530957A/en
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/02Percolation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/24Recirculation of gas

Landscapes

  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE:To obtain the subject device for mass cluturing capabl of being readily operated by installing a vibration pump in a circulating device in a culture solution system and arranging a pipeline in a specific state in the circulating device for tlpe culture solution and a culture gas. CONSTITUTION:A vibration pump 4 is installed in a circulating device 3 for a solution system and a gas-liquid mixed fluid of a culture solution and a culture gas is jetted from a jetting port 5 to produce a vortex. The culture solution is then taken out of a suction port 2 in the central part of the vortex and, together with the circulating gas, as a gas-liquid mixed fluid introduced into a culture tank 1.

Description

【発明の詳細な説明】Detailed Description of the Invention

【0001】[0001]

【産業上の利用分野】本発明は、微生物及び/または細
胞などの培養装置に関するものである。特に液体の流れ
または機械的な作用で培養槽内壁或いは培養槽内のセン
サー類への菌体、藻体或いは動植物細胞等の付着を防止
し、高速、高能率に微生物及び/または細胞を大量培養
する培養装置に関するものである。
BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an apparatus for culturing microorganisms and / or cells. Particularly, the flow of liquid or mechanical action prevents adhesion of bacterial cells, algal cells, animal or plant cells, etc. to the inner wall of the culture tank or the sensors in the culture tank, and mass-cultures microorganisms and / or cells at high speed and with high efficiency. The present invention relates to a culturing device.

【0002】[0002]

【従来の技術】従来の培養装置は培養槽内に培養に必要
な空気等を供給するために散気装置が、また槽内培養液
を均質に混合しガスの溶解効率を高めるために攪拌装置
が設けられたものが主流となっている。また、培養槽内
にドラフトチューブを設け、管内に吹き込む空気の力で
槽内培養液を流動させるエアリフト方式なども開発され
ている。
2. Description of the Related Art A conventional culture apparatus is an air diffuser for supplying air or the like necessary for culture into a culture tank, and a stirring apparatus for uniformly mixing culture solution in the tank to enhance gas dissolution efficiency. It is the mainstream that is provided. In addition, an air lift system has been developed in which a draft tube is provided in the culture tank and the culture liquid in the tank is caused to flow by the force of air blown into the tube.

【0003】現在様々な方式の培養装置が存在するが、
従来の培養装置には培養槽外に特別の気液混合手段を設
けることなく、かつ培養液供給ポンプ内で強い剪断流を
与えることなく、気液混合流体を培養槽に供給する手段
がなかったので、培養槽内に散気装置を設置し、かつ培
養槽内の培養液に強い剪断流を与える攪拌手段を設ける
ことによって培養に必要な空気等のガスを供給してきた
のである。
Currently, there are various types of culture devices,
The conventional culture device has no means for supplying the gas-liquid mixed fluid to the culture tank without providing a special gas-liquid mixing means outside the culture tank and without giving a strong shear flow in the culture liquid supply pump. Therefore, a gas such as air necessary for culture has been supplied by installing an air diffuser in the culture tank and by providing a stirring means for giving a strong shear flow to the culture solution in the culture tank.

【0004】しかしながら、これら従来の培養装置には
次に列挙するような技術的問題点があり、これらの問題
点を改善するために各研究機関で各種の研究が行われて
いる。
However, these conventional culture devices have the following technical problems, and various researches are being carried out at each research institute in order to improve these problems.

【0005】(技術的問題点) 1.培養槽内に散気装置、攪拌装置、及びセンサー類な
ど様々な備品が存在しているため、培養槽内培養液の流
れに滞留部分が多く生じ、そこへ培養菌体或いは細胞が
濃厚に付着し、精度の高い実験データを取得することに
著しい障壁となっている。
(Technical problems) 1. Since various equipment such as an air diffuser, agitator, and sensors exist in the culture tank, a large amount of stagnation occurs in the flow of the culture solution in the culture tank, and the cultured cells or cells adhere to it in a concentrated manner. However, it is a significant obstacle to obtaining highly accurate experimental data.

【0006】2.付着を防止するため攪拌装置で培養液
に強い剪断流を起こす方法は、強度の弱い細胞などには
不適格である。
2. The method of causing a strong shear flow in the culture solution with a stirring device in order to prevent adhesion is not suitable for cells having low strength.

【0007】[0007]

【発明が解決しようとする課題】前記した従来の培養装
置には菌体、藻体或いは細胞等の付着という大きな問題
を解決する手段を備えていない。本発明者らは、従来の
培養装置の持つこれらの問題点を改善し、従来の培養装
置と比較して格段の大量培養を可能にする、かつ運転操
作も著しく容易な新規な培養装置を提供しようとするも
のである。
The above-mentioned conventional culture device is not provided with means for solving the big problem of adhesion of bacterial cells, algal cells or cells. The present inventors provide a novel culturing apparatus that improves these problems of the conventional culturing apparatus, enables significantly large-scale culturing as compared with the conventional culturing apparatus, and is significantly easy to operate. Is what you are trying to do.

【0008】[0008]

【課題を解決するための手段及び作用】前記課題は、 1)少なくとも培養槽並びに培養液系及び培養ガス系を
有する循環装置を備えた培養装置において、少なくとも
前記培養液系循環装置中に振動ポンプを設置し、前記振
動ポンプによって前記培養槽内の培養液及び培養ガスを
気液混合流体として培養槽内下部の壁面から噴出し、培
養槽内の培養液上部の渦中心部から培養液を引き抜いて
循環ガスとともに気液混合流体として再び培養槽内に高
速で導入して、培養槽内の培養液に渦流を起こさしめる
ように培養液系と培養ガス系との循環装置を配備するこ
とを特徴とする培養装置。
Means and Actions for Solving the Problems The above problems are as follows: 1) In a culture device equipped with at least a culture tank and a circulation device having a culture solution system and a culture gas system, at least a vibration pump in the culture solution system circulation device. The culture solution and the culture gas in the culture tank are jetted as a gas-liquid mixed fluid from the wall surface of the lower part of the culture tank by the vibration pump, and the culture solution is drawn out from the center of the vortex above the culture solution in the culture tank. It is characterized in that a circulation device for the culture solution system and the culture gas system is installed so that it is introduced into the culture tank at high speed again as a gas-liquid mixed fluid together with the circulating gas, and a vortex is caused in the culture solution in the culture tank. And a culture device.

【0009】2)前記培養槽内の培養液中に比重が水よ
り重い担体を混入して循環装置で培養液と共に循環し、
培養槽内壁へ付着する菌体等を除去することを特徴とす
る前記1)に記載の培養装置。
2) A carrier having a specific gravity heavier than water is mixed in the culture solution in the culture tank and circulated with the culture solution in a circulation device,
The culture device according to 1) above, which removes bacterial cells and the like adhering to the inner wall of the culture tank.

【0010】3)前記培養槽内の培養液中に循環装置に
入らない大きさを持ったスポンジ状の担体を混入して培
養槽内を循環し、培養槽内壁へ付着する菌体等を除去す
ることを特徴とする前記1)に記載の培養装置によって
達成される。
3) A sponge-like carrier having a size that does not enter the circulation device is mixed into the culture solution in the culture tank to circulate in the culture tank to remove bacterial cells and the like adhering to the inner wall of the culture tank. It is achieved by the culture device according to 1) above.

【0011】本発明において、培養装置の循環装置に設
置する振動ポンプとは、例えば特公平3−13439号
公報及び「配管と装置」4月号(1991)31〜36
頁に記載されている端部にばね弁を配した円筒直管を管
軸方向に振動させ、管内流体の共振現象を有効に利用し
た揚液効果に基づくポンプであり、培養装置の循環装置
に組み込まれるのは本発明が最初である。
In the present invention, the vibration pump installed in the circulation device of the culture device is, for example, Japanese Patent Publication No. 3-13439 and "Piping and Device" April issue (1991) 31-36.
It is a pump based on the pumping effect that effectively utilizes the resonance phenomenon of the fluid in the pipe by vibrating the cylindrical straight pipe with the spring valve arranged at the end on the page in the pipe axial direction. The present invention is the first to be incorporated.

【0012】培養装置の循環装置にこの革新的な振動ポ
ンプを採用すると共に培養液系と培養ガス系との循環装
置の配備を改良して、培養槽内から攪拌装置や散気管の
設置を不必要とし、かつ培養槽内の培養液に高速流を起
こし培養槽壁面及び各種センサー類への菌体等の付着を
防ぎ、さらに好ましくは担体を培養液に混合することに
より培養槽壁面及び各種センサー類に付着した菌体等を
除去して課題を解決した。
By adopting this innovative vibration pump as the circulation device of the culture device and improving the arrangement of the circulation device for the culture solution system and the culture gas system, it is not necessary to install a stirring device or an air diffusing tube from the culture tank. If necessary, a high-speed flow is caused in the culture solution in the culture tank to prevent the adherence of bacterial cells to the culture tank wall surface and various sensors, and more preferably by mixing a carrier with the culture solution, the culture tank wall surface and various sensors The problem was solved by removing the bacterial cells and the like attached to the species.

【0013】前記培養装置の循環装置に振動ポンプを設
置するに当たって、少なくとも前記培養液系循環装置中
に振動ポンプを設置するということは、培養装置の循環
装置の培養液系及び培養ガス系からなる2系統の循環装
置は1系統に接続されて培養槽に入るが、その培養ガス
系循環装置を培養液系循環装置と接続する位置が振動ポ
ンプの前後何れでもよく、また前後何れにおいても接続
していてもよいことを意味する。
When installing the vibration pump in the circulation device of the culture device, at least installing the vibration pump in the culture liquid circulation device means the culture liquid system and the culture gas system of the culture device circulation device. The two circulation devices are connected to one culture system and enter the culture tank, but the position where the culture gas circulation device is connected to the culture liquid circulation device may be before or after the vibration pump, and may be connected before or after the vibration pump. Means that it may be.

【0014】培養液系循環装置中に循環している培養液
は通常は気液混合流体として循環して、この気液混合流
体は振動ポンプによって培養液中にガスが効率よく溶解
される。もし振動ポンプの後部で培養ガスが添加された
ときは噴出時に液中に分散され気液混合流体となり、そ
の気液混合流体が循環して振動ポンプに達した時に培養
液中にガスがよりよく溶解されることになる。
The culture solution circulated in the culture solution circulation apparatus is usually circulated as a gas-liquid mixed fluid, and the gas is efficiently dissolved in the culture solution by the vibration pump. If culture gas is added at the rear of the vibration pump, it will be dispersed in the liquid at the time of jetting and become a gas-liquid mixed fluid, and when the gas-liquid mixed fluid circulates and reaches the vibration pump, the gas in the culture liquid will be better. Will be dissolved.

【0015】培養装置に設置される液循環用のポンプと
して具備すべき性能としては、1)培養槽内のできるだ
け大量の培養液を均一かつ強力に攪拌できること。2)
菌体及び細胞等の培養に必要な空気、二酸化炭素(CO
2 )富化空気及び/または二酸化炭素を培養液へ効率よ
く溶解できること。3)攪拌中に菌体及び細胞等に損傷
を与えないこと。及び4)液循環装置が滅菌処理できる
こと等である。
The performances that should be provided as a liquid circulation pump installed in the culture device are as follows: 1) The culture liquid in the culture tank can be stirred uniformly and strongly as much as possible. 2)
Air and carbon dioxide (CO) necessary for culturing cells and cells
2 ) To be able to efficiently dissolve enriched air and / or carbon dioxide into the culture solution. 3) Do not damage the cells and cells during stirring. And 4) The liquid circulation device can be sterilized.

【0016】液循環用のポンプとしは従来例えばローラ
ーチューブポンプやダイヤフラムポンプ等が挙げられ
る。 ローラーチューブポンプは送液に使用されるチュ
ーブがオートクレーブ滅菌できる特徴を有するが、処理
できる液量が小さく、また循環操作中に加圧操作が含ま
れるため菌体及び細胞等に損傷を与える懸念がある等の
問題点を有する。また、ダイヤフラムポンプは大量の培
養液を均一かつ強力に攪拌することができるが、ポンプ
自体をオートクレーブ処理をすることができないため別
個に接液部に蒸気を通して滅菌しなくてはならず、しか
もその際弁が2箇所にあるため完全な滅菌はできない等
の問題点を有する。さらにこれらローラーチューブポン
プやダイヤフラムポンプはポンプ内部では空気等の培養
ガスを培養液に溶解するための積極的作用はない。
As the pump for circulating the liquid, conventionally, for example, a roller tube pump, a diaphragm pump and the like can be mentioned. The roller tube pump has a feature that the tube used for liquid feeding can be sterilized by autoclave, but the amount of liquid that can be processed is small, and since pressure operation is included during circulation operation, there is a concern that it will damage cells and cells. There are some problems. In addition, the diaphragm pump can uniformly and strongly agitate a large amount of culture solution, but since the pump itself cannot be autoclaved, it must be sterilized by passing steam separately through the liquid contact part. There is a problem that complete sterilization cannot be performed because there are two veneer valves. Furthermore, these roller tube pumps and diaphragm pumps do not have a positive action for dissolving the culture gas such as air in the culture solution inside the pumps.

【0017】本発明の振動ポンプは、前記した通り管内
流体の共振現象を応用した揚液効果に基づく全く新しい
形式のポンプであり、以下に述べる如く培養装置に最適
のポンプであり、培養装置の循環装置として極めて優れ
た性能を発揮する。
The vibration pump of the present invention is a completely new type of pump based on the pumping effect that applies the resonance phenomenon of the fluid in the pipe as described above, and is the optimum pump for the culture device as described below. Excellent performance as a circulation device.

【0018】すなわち、振動ポンプはポンプ自体をオー
トクレーブ処理できるので完全な滅菌ができ、構造がシ
ンプルなため内部の洗浄も容易である。大流量の培養液
の循環が可能であり、循環操作中に菌体及び細胞等に損
傷を与える懸念は全くない。
That is, the vibration pump can be completely sterilized because the pump itself can be autoclaved, and the internal structure is easy to clean because of its simple structure. It is possible to circulate a large amount of culture solution, and there is no fear of damaging the cells and cells during the circulation operation.

【0019】また、振動ポンプではポンプ内で培養液と
培養ガスが充分に攪拌混合されるため、前記従来の他の
ポンプでは見られない作用を示す。すなわち循環装置内
を流れる、培養液と菌体及び細胞等の培養に必要な空気
や二酸化炭素からなる培養ガスとの気液混合流体がポン
プ内で充分に攪拌混合され培養ガスの培養液への溶解が
ポンプから培養槽に到達する間に十分に行われるという
特徴を有する。
Further, in the vibration pump, the culture solution and the culture gas are sufficiently agitated and mixed in the pump, so that there is an action not seen in the other conventional pumps. That is, a gas-liquid mixed fluid of a culture solution and a culture gas consisting of air and carbon dioxide necessary for culturing cells, cells, etc., flowing in the circulation device is sufficiently agitated and mixed in a pump to form a culture gas of the culture solution. It is characterized in that the lysis is sufficiently performed while it reaches the culture tank from the pump.

【0020】もっとも本培養装置の場合に、空気や二酸
化炭素からなる培養ガスの培養液への溶解が、他の培養
装置の場合より一層効率良く行われる所以は、本振動ポ
ンプの性能による他、本発明の改良された培養槽内への
気液混合流体の噴出と槽内に生起した流体の渦の中心か
らの吸入により培養槽内の液に安定した渦流が生ずるこ
と、及び本培養装置の循環方法では培養液が繰り返し培
養槽に導入されるという本発明の循環装置の配備方法に
も依存している。
However, in the case of the main culturing apparatus, the reason why the culturing gas consisting of air and carbon dioxide is dissolved more efficiently in the culturing solution than in the case of other culturing apparatuses is due to the performance of the present vibration pump, A stable vortex flow is generated in the liquid in the culture tank by jetting the gas-liquid mixed fluid into the improved culture tank of the present invention and inhaling from the center of the vortex of the fluid generated in the tank, and The circulation method also depends on the method of deploying the circulation device of the present invention in which the culture solution is repeatedly introduced into the culture tank.

【0021】さらに振動ポンプは、培養装置に菌体、藻
体或いは細胞等を付着させないという重要課題解決のた
めに大きな役割を果たしている。 すなわち、前記した通り振動ポンプは大流量の培養液
の循環が可能であり、かつ菌体及び細胞等の培養に必要
な空気や二酸化炭素の培養液への溶解が非常に効率良く
行われるために、本発明の培養装置には装置中に攪拌装
置や散気装置を設置する必要がなく、従って培養槽内の
構造が極めてシンプルにできる。これと同時に振動ポン
プ及び付帯循環管路の構造も極めてシンプルなので本発
明の培養装置には装置中に菌体、藻体或いは細胞等が付
着する箇所が極めて少なくできる。
Further, the vibration pump plays a major role in solving the important problem of preventing bacterial cells, algal cells or cells from adhering to the culture device. That is, as described above, the vibration pump is capable of circulating a large amount of the culture solution, and because the air and carbon dioxide necessary for culturing cells and cells are dissolved in the culture solution very efficiently. The culture device of the present invention does not require a stirring device or an air diffuser to be installed in the device, so that the structure in the culture tank can be made extremely simple. At the same time, since the structure of the vibration pump and the auxiliary circulation line is extremely simple, the culture device of the present invention can have very few places where cells, algae, cells or the like adhere to the device.

【0022】本発明の培養装置に設置される液系循環
装置では振動ポンプの使用によって培養槽内の培養液中
に比重が水より重い担体を混入して液系循環装置で培養
液と共に循環することができるため、これら担体によっ
て装置中に付着した菌体、藻体或いは細胞等を除去でき
る。
In the liquid circulation device installed in the culture apparatus of the present invention, a carrier having a specific gravity higher than that of water is mixed into the culture liquid in the culture tank by using a vibration pump, and is circulated together with the culture liquid in the liquid circulation device. Therefore, the cells, algal cells, cells and the like attached to the device can be removed by these carriers.

【0023】培養装置に菌体、藻体或いは細胞等を付着
させないという重要課題解決のためには振動ポンプの採
用のほかに、本発明の培養液系と培養ガス系との循環装
置の配備における改良も重要な要因であった。すなわ
ち、培養液とガスとからなる気液混合流体を培養槽内下
部の壁面から噴出し、好ましくは槽内壁の接線方向に噴
出し、培養槽内の培養液上部の渦中心部から培養液を引
き抜いて循環ガスとともに気液混合流体として培養液系
循環装置内を通って、再び前記培養槽内に高速で導入し
て、前記培養槽内の培養液に渦流を起こさしめるように
培養液系と培養ガス系との循環装置を配備することによ
って課題の達成が可能となった。この繰り返し気液混合
流体を培養槽内に導入することが培養に必要な空気や二
酸化炭素の培養液への溶解を非常に効率良く行うことに
寄与している。
In order to solve the important problem of not adhering bacterial cells, algae, cells or the like to the culture device, in addition to the adoption of a vibration pump, a circulation device for the culture solution system and the culture gas system of the present invention is provided. Improvement was also an important factor. That is, a gas-liquid mixed fluid consisting of a culture solution and gas is ejected from the wall surface of the lower part of the culture tank, preferably in the tangential direction of the inner wall of the culture tank, and the culture solution is ejected from the center of the vortex above the culture solution in the culture tank. It is drawn out and passed through a culture fluid system circulation device as a gas-liquid mixed fluid together with a circulating gas, and is again introduced into the culture vessel at a high speed, and a culture fluid system is used so as to cause a swirl in the culture fluid in the culture vessel. The task could be achieved by providing a circulation device with the culture gas system. The repeated introduction of the gas-liquid mixed fluid into the culture tank contributes to the very efficient dissolution of air and carbon dioxide required for the culture into the culture solution.

【0024】前記革新的な振動ポンプの採用を含む本発
明の特徴ある循環装置によって、また培養槽内の培養液
中に比重が水より重い担体を混入して或いは循環装置に
入らない大きさを持ったスポンジ状の担体を混入し培養
槽内に付着した菌体、藻体或いは細胞等を除去すること
によって、培養装置に関する重要な問題点が解決され
た。
With the characteristic circulation device of the present invention including the adoption of the above-mentioned innovative vibration pump, and the size of the culture liquid in the culture tank in which a carrier having a specific gravity higher than that of water is mixed or the liquid does not enter the circulation device. By mixing the sponge-like carrier with the cells and removing the cells, algae, cells, etc. adhering to the inside of the culture tank, an important problem regarding the culture apparatus was solved.

【0025】ここで、前記培養液中に混入させる比重が
水より重い担体の具体例としては前記スポンジ状の担体
の他に、人工骨材、砂粒などが挙げられる。また、担体
の比重としては1〜2の値のものが好ましく用いられ
る。
Here, specific examples of the carrier having a specific gravity heavier than water to be mixed in the culture solution include artificial sponge and sand grains in addition to the sponge-like carrier. Further, the carrier having a specific gravity of 1 to 2 is preferably used.

【0026】[0026]

【具体的態様及び作用の説明】以下に図1、2、3及び
4を用いて本発明の特徴及びその優れた作用をより具体
的に説明する。ただし本発明は以下の具体的説明によっ
て制限されるものではない。
DESCRIPTION OF SPECIFIC EMBODIMENTS AND OPERATION The features of the present invention and the excellent operation thereof will be described in more detail below with reference to FIGS. However, the present invention is not limited to the following specific description.

【0027】図1は、本発明の培養装置の説明図であ
る。培養槽1内に培養液と培養するべき菌体、藻体或い
は細胞等を仕込み、培地供給管8より必要な栄養成分を
添加される。培養ガス供給管7から培養に必要な空気、
二酸化炭素富化空気及び/または二酸化炭素を供給する
とこれらガスはガス系循環装置6を通って培養液に供給
される。培養液は液系循環装置3を通って培養槽1及び
液系循環装置3中を循環する。同時に培養ガスはガス系
循環装置6を循環する。振動ポンプ4では前記液系循環
装置3からの培養液とガス系循環装置6からの培養ガス
が混合して気液混合流体が形成され、培養槽1内下部の
壁面にある気液混合流体の噴出口5から培養槽1内壁の
接線方向に噴出し、気液混合流体は渦状に培養槽1内を
上昇し、培養槽1上部にあり培養液の渦中心部に開口し
ている培養液の吸引口2から培養液が引き抜かれて循環
ガスとともに気液混合流体として再び振動ポンプによっ
て高速で培養槽1内に戻り、以降培養装置内を循環す
る。
FIG. 1 is an explanatory view of the culture device of the present invention. A culture solution and fungi, algae or cells to be cultured are charged into the culture tank 1, and necessary nutrient components are added from the medium supply pipe 8. Air required for culture from the culture gas supply pipe 7,
When the carbon dioxide-enriched air and / or carbon dioxide is supplied, these gases are supplied to the culture solution through the gas system circulation device 6. The culture liquid circulates in the culture tank 1 and the liquid circulation device 3 through the liquid circulation device 3. At the same time, the culture gas circulates in the gas system circulation device 6. In the vibration pump 4, the culture solution from the liquid circulation device 3 and the culture gas from the gas circulation device 6 are mixed to form a gas-liquid mixed fluid. The gas-liquid mixed fluid is jetted from the jet outlet 5 in the tangential direction of the inner wall of the culture tank 1, rises in the culture tank 1 in a vortex shape, and the gas mixture of the culture solution above the culture tank 1 and opening at the center of the culture solution vortex The culture solution is drawn out from the suction port 2 and returns to the culture tank 1 at high speed again as a gas-liquid mixed fluid together with the circulating gas by the vibration pump, and then circulates in the culture apparatus.

【0028】この前記培養液の培養槽1の内壁の接線方
向への噴出と培養槽1上部の渦中心部の開口からの培養
液の吸引によって培養槽1内には旋回流を生成せしめる
ことによって、培養槽1の内壁表面及び培養槽1に挿入
されている計測用センサー(図示されていない)等の周
りに常に水流が起こるため培養槽1の内壁表面及び培養
槽1に挿入されている計測用センサーへの培養菌体の付
着を防止することができる。培養に必要な空気、二酸化
炭素富化空気及び/または二酸化炭素はガス系循環装置
6に連続的または間欠的に導入され、同量のガスがガス
排出管10から排出される。空気、二酸化炭素富化空気
及び/または二酸化炭素は繰り返し培養槽1内に導入さ
れるため、培養液への培養ガスの溶解効率が著しく高め
られる。
By squirting the culture solution in the tangential direction of the inner wall of the culture tank 1 and sucking the culture solution from the opening at the center of the vortex in the upper part of the culture tank 1, a swirl flow is generated in the culture tank 1. The measurement of the inner wall surface of the culture tank 1 and the measurement inserted into the culture tank 1 because a water flow always occurs around the inner wall surface of the culture tank 1 and a measuring sensor (not shown) inserted in the culture tank 1. It is possible to prevent adherence of cultured bacterial cells to the sensor for use. Air, carbon dioxide-enriched air, and / or carbon dioxide necessary for culture are continuously or intermittently introduced into the gas system circulation device 6, and the same amount of gas is discharged from the gas discharge pipe 10. Since air, carbon dioxide-enriched air, and / or carbon dioxide are repeatedly introduced into the culture tank 1, the dissolution efficiency of the culture gas in the culture solution is significantly enhanced.

【0029】吸引口2から引き抜かれた培養液は液系循
環装置3を通り、ガス系循環装置6の循環ガスとあわせ
て気液混合流体として振動ポンプ4によって噴出口5よ
り高速で培養槽内に導入される。この際ガス系循環装置
6は振動ポンプ4の吸い込み側に接続するのが効率的で
あるが、ポンプ4の出口側に接続してもよい。培養され
た菌体、藻体或いは細胞等は培養液流出管9から取り出
される。
The culture solution withdrawn from the suction port 2 passes through the liquid system circulation device 3, and together with the circulation gas of the gas system circulation device 6 as a gas-liquid mixed fluid by the vibration pump 4 at a higher speed than the jet port 5 in the culture tank. Will be introduced to. At this time, it is efficient to connect the gas system circulation device 6 to the suction side of the vibration pump 4, but it may be connected to the outlet side of the pump 4. The cultured bacterial cells, algal cells, cells or the like are taken out from the culture solution outflow pipe 9.

【0030】図2は、噴出口5から培養槽1内壁の接線
方向に噴出される水流に関する説明図である。培養槽1
内壁、センサー類等への培養菌体、藻体或いは細胞の付
着を防止するためには接線方向での培養液の流速が50
cm/sec以上であることが望ましい。また、付着防
止効果を一層高めるために流速を定期的に変化させても
良い。
FIG. 2 is an explanatory view of the water flow ejected from the ejection port 5 in the tangential direction of the inner wall of the culture tank 1. Culture tank 1
The flow velocity of the culture solution in the tangential direction is 50 in order to prevent adherence of cultured bacterial cells, algal cells or cells to the inner wall or sensors.
It is preferably cm / sec or more. In addition, the flow rate may be changed periodically in order to further enhance the adhesion prevention effect.

【0031】図3は、培養液中に水より比重の重い担体
11を混合して液系循環装置3で培養液と共に循環し、
培養槽1内壁、センサー類等への培養菌体等の付着物を
除去する場合の培養装置の関する説明のための模式図で
ある。
In FIG. 3, a carrier 11 having a higher specific gravity than water is mixed in the culture solution and circulated with the culture solution in the liquid circulation device 3,
FIG. 3 is a schematic diagram for explaining a culture device for removing adhered substances such as cultured bacterial cells to the inner wall of the culture tank 1 and sensors.

【0032】担体11は比重が水より重いため、渦流に
よって生じる遠心力により培養槽1内壁に押しつけられ
つつ壁面を上昇し機械的なこすり作用によって付着物除
去の効果を発揮する。
Since the specific gravity of the carrier 11 is heavier than that of water, the carrier 11 is pressed against the inner wall of the culture tank 1 by the centrifugal force generated by the vortex flow and ascends the wall surface, thereby exerting the effect of removing the deposits by the mechanical rubbing action.

【0033】振動ポンプ4は粒子が混入している液体で
も送液が可能であり、本発明の目的に対して優れた効果
を発揮する。ただし、このためには担体11の粒子の直
径が2mm以下であることが望ましい。
The vibration pump 4 is capable of feeding even a liquid containing particles, and exhibits an excellent effect for the purpose of the present invention. However, for this purpose, the diameter of the particles of the carrier 11 is preferably 2 mm or less.

【0034】図4は、培養液中に液系循環装置3には入
れない大きさをもったスポンジ状の担体を混合して培養
槽1内壁、センサー類等への培養菌体等の付着物を除去
する場合の培養装置の関する説明のための模式図であ
る。
FIG. 4 shows that a sponge-like carrier having a size that cannot be put in the liquid circulation device 3 is mixed in the culture solution to attach substances such as cultured cells to the inner wall of the culture tank 1 and sensors. FIG. 3 is a schematic diagram for explaining a culture device when removing a bacterium.

【0035】培養槽1上部にあり培養液の渦中心部に開
口している培養液のスクリーン付き吸引口12にはスク
リーンが設けられ、液系循環装置3に担体が流入するこ
とを防止する。振動ポンプ4は前述した通り、揚液原理
により培養液に脈動流を与えるためスポンジ状の担体が
吸引口12のスクリーンに押しつけられてこれを閉塞す
ることを回避できる。担体は渦流の中心部で培養槽1の
上部から下部へ移行し、再び渦流によって生じる遠心力
により培養槽1内壁に押しつけられつつ壁面を上昇し機
械的なこすり作用によって付着物除去の効果を発揮す
る。
A screen is provided at the screen-equipped suction port 12 of the culture solution, which is located at the upper part of the culture tank 1 and opens at the center of the vortex of the culture solution, to prevent the carrier from flowing into the liquid circulation device 3. As described above, the vibration pump 4 gives a pulsating flow to the culture solution by the pumping principle, so that it is possible to prevent the sponge-like carrier from being pressed against the screen of the suction port 12 and blocking it. The carrier migrates from the upper part to the lower part of the culture tank 1 at the center of the vortex flow, and is again pressed against the inner wall of the culture tank 1 by the centrifugal force generated by the vortex flow to rise the wall surface and exert the effect of removing the deposits by the mechanical rubbing action To do.

【0036】この渦流によって生じる遠心力により培養
槽1内壁に押しつけられる作用を一層高めるため、ここ
に使用する担体の比重を水より大きいものとし、通常は
沈降している当該担体を、定期的に培養液の噴出流の流
速を増大させて、浮上させ機械的なこすり作用を発揮さ
せても良い。
In order to further enhance the action of being pressed against the inner wall of the culture tank 1 by the centrifugal force generated by this vortex, the specific gravity of the carrier used here is made larger than that of water, and the normally precipitated carrier is periodically removed. It is also possible to increase the flow velocity of the jet of the culture solution to make it float and exert a mechanical rubbing action.

【0037】[0037]

【実施例】一般的な緑藻であるクロレラピレノイドーサ
(Chlorellapyenoidosa)の培養実験を培養槽の規模を
2リットルとして、 A)最大流量3リットル/minの前記「配管と装置」
4月号(1991)31〜36頁に4段可動永久磁石形
ポンプとして記載されている振動ポンプ4を設置した図
5に示す本発明の培養装置、および B)散気管4と攪拌子3を装備した図6に示した比較例
の培養装置を用いて行った。
[Examples] A culture experiment of a general green alga, Chlorella pyenoidosa, was carried out with a culture tank scale of 2 liters. A) The maximum flow rate of 3 liters / min.
In the April issue (1991) pages 31 to 36, the culturing apparatus of the present invention shown in FIG. 5 in which a vibrating pump 4 described as a four-stage movable permanent magnet pump is installed, and B) an air diffusing tube 4 and an agitator 3. The culture was performed using the equipped culture apparatus of the comparative example shown in FIG.

【0038】培養ガスは炭酸ガスを5%混合した空気を
用い、培養液pHは8.0、培養液の温度は25℃と
し、メタルハライドランプを用いて槽外より光を(培養
槽外壁面で照度5000ルックスの条件で)照射しつつ
3週間、回分培養した。
The culture gas used was air mixed with 5% carbon dioxide, the pH of the culture solution was 8.0, the temperature of the culture solution was 25 ° C., and light was emitted from the outside of the tank using a metal halide lamp (on the outer wall surface of the culture tank). Batch culture was carried out for 3 weeks under irradiation (under an illumination of 5000 lux).

【0039】培地は次に示すMC培地を用いた。 KNO3 1.25g MgSO4 1.25g KH2 PO4 1.25g A5 Solution 1 ml FeSolution 1 ml 蒸留水 1 リットル (実施例1)図5において、培養槽内培養液は振動ポン
プを用いて循環流量(QL )2リットル/minで循環
した。培養ガスは(QG )1リットル/minで循環
し、30分間に5分間電磁弁13を切換えて新鮮ガスを
1リットル/minで供給した。
The MC medium shown below was used as the medium. KNO 3 1.25 g MgSO 4 1.25 g KH 2 PO 4 1.25 g A 5 Solution 1 ml FeSolution 1 ml Distilled water 1 liter (Example 1) In FIG. 5, the culture solution in the culture tank was circulated using a vibration pump. flow rate was circulated in the (Q L) 2 Rittoru / min. The culture gas was circulated at (Q G ) 1 liter / min, and the solenoid valve 13 was switched for 5 minutes for 30 minutes to supply fresh gas at 1 liter / min.

【0040】本発明の装置において、 最大増殖速度 0.20g/時間・リットル、 最終浮遊菌体濃度 7.0 g/リットル が得られた。In the device of the present invention, Maximum growth rate 0.20 g / hour · liter, Final suspended cell concentration: 7.0 g / liter was gotten.

【0041】壁面藻体付着量は全量で 約0.5gであ
った。 (比較例1)図6において、培養ガスは散気管4より1
リットル/minで供給した。
The amount of algal cells attached to the wall surface was about 0.5 g in total. (Comparative example 1) In FIG.
It was supplied at liter / min.

【0042】本比較例の装置において、 最大増殖速度 0.12g/時間・リットル、 最終浮遊菌体濃度 2.0 g/リットル が得られた。In the apparatus of this comparative example, Maximum growth rate 0.12g / hour ・ liter, Final suspended cell concentration 2.0 g / l was gotten.

【0043】壁面藻体付着量は全量で 約3.0gであ
った。
The amount of algal cells on the wall surface was about 3.0 g in total.

【0044】[0044]

【発明の効果】【The invention's effect】

1)本発明の、振動ポンプの使用と培養液及びガスの循
環装置の管路の配置とによって、培養槽内に培養液の旋
回流を生じ、培養槽の内壁及び槽内各種センサーへの培
養菌体等の付着を防止できた。
1) The use of the vibration pump of the present invention and the arrangement of the channels of the circulation device for the culture solution and the gas cause a swirling flow of the culture solution in the culture tank, and culture on the inner wall of the culture tank and various sensors in the tank. It was possible to prevent the adherence of bacterial cells.

【0045】2)本発明の、振動ポンプの使用と培養液
及びガスの循環装置の管路の配置とによって、培養液と
培養ガスからなる気液混合流体の培養槽内及び循環装置
の管路内への繰り返し循環流通が行われ、空気、二酸化
炭素富化空気及び/または二酸化炭素は十分に培養液に
溶解されるので、本発明の培養装置では槽中に攪拌装置
や散気管が不要で、装置がシンプルにでき、かつ培養槽
の内壁及び槽内各種センサーへの培養菌体等の付着を防
止できた。
2) By using the vibration pump of the present invention and arranging the conduits of the circulation device for the culture solution and gas, the conduits of the circulation device for the gas-liquid mixed fluid consisting of the culture solution and the culture gas are provided. As a result of repeated circulation to the inside, air, carbon dioxide-enriched air and / or carbon dioxide are sufficiently dissolved in the culture solution, the culture apparatus of the present invention does not require a stirring device or an air diffusing tube in the tank. The device was simple, and the adherence of cultured bacterial cells to the inner wall of the culture tank and various sensors in the tank could be prevented.

【0046】3)本発明で使用する振動ポンプは、オー
トクレーブ滅菌が可能で、ポンプの構造及び付属管路も
シンプルなので、洗浄が容易であり、菌体や藻体等をポ
ンプ中に通してもそれらが破損する懸念がなく、培養装
置用ポンプに適している。
3) The vibration pump used in the present invention can be sterilized by autoclave, and the structure of the pump and the attached pipeline are simple, so that it is easy to wash, and even if bacteria or algae are passed through the pump. It is suitable for pumps for culture devices because there is no risk of damage to them.

【0047】4)本発明で使用する振動ポンプ内では、
培養液とガスとが十分に攪拌混合されるので、これら気
液混合流体が培養槽に達するまでにガスは良く培養液中
に溶解される。
4) In the vibration pump used in the present invention,
Since the culture solution and the gas are sufficiently stirred and mixed, the gas is well dissolved in the culture solution by the time the gas-liquid mixed fluid reaches the culture tank.

【0048】5)本発明で使用する振動ポンプ内では、
培養液が細かい担体を含んでいても大流量を確保できる
ので、水より比重の重い担体を培養液に混入させて培養
槽内に循環して培養槽の内壁及び槽内各種センサーへの
培養菌体等の付着物を除去できた。
5) In the vibration pump used in the present invention,
Since a large flow rate can be secured even if the culture solution contains a fine carrier, the carrier with a higher specific gravity than water is mixed into the culture solution and circulated in the culture tank to culture bacteria on the inner wall of the culture tank and various sensors in the tank. The deposits such as the body could be removed.

【0049】6)培養液中に循環装置に入らない大きさ
を持ったスポンジ状の担体を培養液に混入させて培養槽
内に循環して培養槽の内壁及び槽内各種センサーへの培
養菌体等の付着物を除去できた。
6) A sponge-shaped carrier having a size that does not enter the circulation device in the culture solution is mixed with the culture solution and circulated in the culture tank to culture the bacteria on the inner wall of the culture tank and various sensors in the tank. The deposits such as the body could be removed.

【図面の簡単な説明】[Brief description of drawings]

【図1】図1は、本発明の培養装置の説明図である。FIG. 1 is an explanatory view of a culture device of the present invention.

【図2】図2は本発明の装置の培養槽内壁の接線方向に
噴出される水流に関する説明図である。
FIG. 2 is an explanatory diagram relating to a water flow ejected in the tangential direction of the inner wall of the culture tank of the device of the present invention.

【図3】図3は培養液中に水より比重の重い担体を培養
液と共に混合して循環しする培養装置の説明図である。
FIG. 3 is an explanatory diagram of a culture device in which a carrier having a higher specific gravity than water is mixed with the culture solution and circulated in the culture solution.

【図4】図4は培養液中に循環装置に入らない大きさを
持ったスポンジ状の担体を培養液に混合して循環しする
培養装置の説明図である。
FIG. 4 is an explanatory diagram of a culture device in which a sponge-like carrier having a size that does not enter the circulation device in the culture medium is mixed with the culture medium and circulated.

【図5】図5は、実施例1において用いた培養装置の説
明図である。
FIG. 5 is an explanatory diagram of a culture device used in Example 1.

【図6】図6は、比較例1において用いた培養装置の説
明図である。
FIG. 6 is an explanatory diagram of a culture device used in Comparative Example 1.

【符号の説明】[Explanation of symbols]

1 培養槽 2 吸引口 3 液系循環装置 4 振動ポンプ 5 噴出口 6 ガス系循環装置 7 培養ガス供給管 8 培地供給管 9 培養液流出管 10 ガス排出管 11 担体 12 スクリーン付き吸引口 13 液流を示す矢印 14 電磁弁 15 比較例の培養槽 16 マグネットスターラー 17 攪拌子 18 散気管 19 比較例の培養ガス供給管 20 比較例のガス排出管 QL 培養液循環流量 QG 培養ガス循環流量1 Culture Tank 2 Suction Port 3 Liquid Circulation Device 4 Vibration Pump 5 Jet 6 Gas System Circulation Device 7 Culture Gas Supply Pipe 8 Medium Supply Pipe 9 Culture Liquid Outflow Pipe 10 Gas Discharge Pipe 11 Carrier 12 Suction Port with Screen 13 Liquid Flow arrow 14 solenoid valve 15 Comparative example gas discharge pipe Q L broth circulation flow rate Q G incubated gas circulation flow rate of the culture gas supply pipe 20 Comparative example diffusing pipe 19 Comparative example fermenter 16 magnetic stirrer 17 stirrer 18 showing the

───────────────────────────────────────────────────── フロントページの続き (72)発明者 梅田 到 神奈川県藤沢市本藤沢4丁目2番1号 株 式会社荏原総合研究所内 (72)発明者 遠矢 泰典 神奈川県藤沢市本藤沢4丁目2番1号 株 式会社荏原総合研究所内 (72)発明者 足立 正 神奈川県藤沢市本藤沢4丁目2番1号 株 式会社荏原総合研究所内 (72)発明者 滝沢 悦子 神奈川県藤沢市本藤沢4丁目2番1号 株 式会社荏原総合研究所内   ─────────────────────────────────────────────────── ─── Continued front page    (72) Inventor Yu Umeda             4-2-1 Honfujisawa, Fujisawa City, Kanagawa Prefecture             Inside the EBARA Research Institute (72) Inventor Yasunori Toya             4-2-1 Honfujisawa, Fujisawa City, Kanagawa Prefecture             Inside the EBARA Research Institute (72) Inventor Tadashi Adachi             4-2-1 Honfujisawa, Fujisawa City, Kanagawa Prefecture             Inside the EBARA Research Institute (72) Inventor Etsuko Takizawa             4-2-1 Honfujisawa, Fujisawa City, Kanagawa Prefecture             Inside the EBARA Research Institute

Claims (3)

【特許請求の範囲】[Claims] 【請求項1】 少なくとも培養槽並びに培養液系及び培
養ガス系を有する循環装置を備えた培養装置において、
少なくとも前記培養液系循環装置中に振動ポンプを設置
し、前記振動ポンプによって前記培養槽内の培養液及び
培養ガスを気液混合流体として培養槽内下部の壁面から
噴出し、培養槽内の培養液上部の渦中心部から培養液を
引き抜いて循環ガスとともに気液混合流体として再び培
養槽内に高速で導入して、培養槽内の培養液に渦流を起
こさしめるように培養液系と培養ガス系との循環装置を
配備することを特徴とする培養装置。
1. A culture device comprising a circulation device having at least a culture tank and a culture solution system and a culture gas system,
A vibration pump is installed in at least the culture liquid circulation device, and the culture liquid and the culture gas in the culture tank are jetted from the wall surface of the lower portion of the culture tank by the vibration pump as a gas-liquid mixed fluid to culture in the culture tank. The culture solution is drawn out from the center of the vortex above the liquid and is again introduced into the culture tank at a high speed as a gas-liquid mixed fluid together with the circulating gas, so that the culture solution in the culture tank and the culture gas can be swirled. A culture device comprising a circulation device with a system.
【請求項2】 前記培養槽内の培養液中に比重が水より
重い担体を混入して前記循環装置で培養液と共に循環
し、前記培養槽内壁へ付着する菌体等を除去することを
特徴とする請求項1に記載の培養装置。
2. The culture medium in the culture tank is mixed with a carrier having a specific gravity heavier than water, and is circulated together with the culture solution in the circulation device to remove bacterial cells and the like adhering to the inner wall of the culture tank. The culture device according to claim 1.
【請求項3】 前記培養槽内の培養液中に前記循環装置
に入らない大きさを持ったスポンジ状の担体を混入して
培養槽内を循環し、前記培養槽内壁へ付着する菌体等を
除去することを特徴とする請求項1に記載の培養装置。
3. A bacterial cell or the like that is circulated in the culture tank by admixing a sponge-like carrier having a size that does not fit in the circulation device into the culture solution in the culture tank and adhering to the inner wall of the culture tank. The culture device according to claim 1, wherein the culture device is removed.
JP19017391A 1991-07-30 1991-07-30 Apparatus for culturing Pending JPH0530957A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP19017391A JPH0530957A (en) 1991-07-30 1991-07-30 Apparatus for culturing

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP19017391A JPH0530957A (en) 1991-07-30 1991-07-30 Apparatus for culturing

Publications (1)

Publication Number Publication Date
JPH0530957A true JPH0530957A (en) 1993-02-09

Family

ID=16253658

Family Applications (1)

Application Number Title Priority Date Filing Date
JP19017391A Pending JPH0530957A (en) 1991-07-30 1991-07-30 Apparatus for culturing

Country Status (1)

Country Link
JP (1) JPH0530957A (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007159496A (en) * 2005-12-14 2007-06-28 Hitoshi Nagaoka Method for dissolving mycelial cell wall and apparatus for dissolving mycelial cell wall used therein
JP2008541741A (en) * 2005-05-30 2008-11-27 ノバルティス ヴァクシンズ アンド ダイアグノスティクス ゲーエムベーハー アンド カンパニー カーゲー Fermenter system for biotechnological processing
JP2011120535A (en) * 2009-12-11 2011-06-23 Ihi Corp Adherent cell culture apparatus
JP2013027361A (en) * 2011-07-29 2013-02-07 Ihi Corp Culture apparatus and culture method
JP2020512844A (en) * 2017-04-13 2020-04-30 ツィテナ ゲーエムベーハーCytena Gmbh Liquid sample processing method
JP2023150307A (en) * 2022-03-31 2023-10-16 本田技研工業株式会社 Culture method and culture apparatus

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008541741A (en) * 2005-05-30 2008-11-27 ノバルティス ヴァクシンズ アンド ダイアグノスティクス ゲーエムベーハー アンド カンパニー カーゲー Fermenter system for biotechnological processing
JP4917597B2 (en) * 2005-05-30 2012-04-18 ノバルティス ヴァクシンズ アンド ダイアグノスティクス ゲーエムベーハー アンド カンパニー カーゲー Fermenter system for biotechnological processing
JP2007159496A (en) * 2005-12-14 2007-06-28 Hitoshi Nagaoka Method for dissolving mycelial cell wall and apparatus for dissolving mycelial cell wall used therein
JP2011120535A (en) * 2009-12-11 2011-06-23 Ihi Corp Adherent cell culture apparatus
JP2013027361A (en) * 2011-07-29 2013-02-07 Ihi Corp Culture apparatus and culture method
JP2020512844A (en) * 2017-04-13 2020-04-30 ツィテナ ゲーエムベーハーCytena Gmbh Liquid sample processing method
US11845044B2 (en) 2017-04-13 2023-12-19 cytena Bioprocess Solutions co., Ltd. Method for processing a liquid sample
JP2023150307A (en) * 2022-03-31 2023-10-16 本田技研工業株式会社 Culture method and culture apparatus

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