JPH05302931A - Spot attaching method of specimen - Google Patents
Spot attaching method of specimenInfo
- Publication number
- JPH05302931A JPH05302931A JP10957792A JP10957792A JPH05302931A JP H05302931 A JPH05302931 A JP H05302931A JP 10957792 A JP10957792 A JP 10957792A JP 10957792 A JP10957792 A JP 10957792A JP H05302931 A JPH05302931 A JP H05302931A
- Authority
- JP
- Japan
- Prior art keywords
- nozzle
- attached
- amount
- spotting
- spotted
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
Description
【0001】[0001]
【産業上の利用分野】本発明は検体の点着方法に関する
ものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for spotting a sample.
【0002】[0002]
【従来の技術】従来、尿,血液等の検体を被点着体に点
着する方法としては、例えば、図4に示すように、特開
平3─37569号公報に開示のものがある。このもの
では、ノズル1と試験紙片2に設けた被点着体3との間
に、液玉4が形成された状態を維持できるような隙間C
を形成し、点着速度を、検体が被点着体3に単位時間当
たりに染み込む量、すなわち展開速度とほぼ等しく設定
している。これにより、点着速度が展開速度よりも大き
くなってノズル1に検体が付着したり、逆に小さくなっ
てノズル1に検体が点着しきれずに残るといった不具合
の発生を防止している。2. Description of the Related Art Conventionally, as a method for spotting a sample such as urine or blood on a spotted object, there is one disclosed in Japanese Patent Laid-Open No. 3-37569, for example, as shown in FIG. In this case, a gap C is formed between the nozzle 1 and the spotted body 3 provided on the test strip 2 so that the state in which the liquid droplet 4 is formed can be maintained.
And the spotting speed is set to be approximately equal to the amount of the sample permeating the spotted object 3 per unit time, that is, the spreading speed. As a result, it is possible to prevent a problem that the spotting speed becomes faster than the developing speed and the specimen adheres to the nozzle 1, or conversely becomes small and the specimen does not remain on the nozzle 1 without being completely spotted.
【0003】[0003]
【発明が解決しようとする課題】しかしながら、前記点
着方法では、ノズル1の先端に液玉4を形成した状態を
維持しながら点着しなければならないため、点着時間が
長くかかる。特に、吸水性能の悪い被点着体3では、さ
らに点着時間が長くなり、検査効率が低下する。また、
検体が被点着体3全体に広がる前に、点着中心部での反
応が進み過ぎるので、点発色や発色むらが発生すること
により、反射光量の測定精度が低下する恐れもある。本
発明は前記問題点に鑑み、所定量の検体を確実かつ高速
で点着でき、しかも、反射光量の測定精度を高めること
のできる検体の点着方法を提供することを目的とする。However, in the spotting method, the spotting time is long because the spotting must be performed while maintaining the state in which the liquid droplet 4 is formed at the tip of the nozzle 1. Particularly, in the adherend 3 to which the water absorption performance is poor, the spotting time becomes longer and the inspection efficiency is lowered. Also,
Before the sample spreads over the whole of the adherend 3, the reaction at the center of the spot proceeds too much, so that spot coloring or uneven coloring may occur, which may reduce the measurement accuracy of the reflected light amount. In view of the above problems, it is an object of the present invention to provide a specimen spotting method capable of spotting a predetermined amount of specimen reliably and at high speed, and further improving the measurement accuracy of the amount of reflected light.
【0004】[0004]
【課題を解決するための手段】本発明は、前記目的を達
成するため、ノズルから被点着体までの間隙寸法をノズ
ル内径の約2/3以下とし、前記ノズルから検体を約
0.5m/s以上で吐出して被点着体に点着することに
より、検体は高圧で被点着体に吐出されるので、被点着
体での展開速度に拘わらず、表面全体に点着され、点発
色や発色むらが軽減できる。また、前記吐出速度は1〜
2m/sとするのが好ましい。In order to achieve the above-mentioned object, the present invention sets the gap size from the nozzle to the spotted object to about 2/3 or less of the inner diameter of the nozzle, and the sample from the nozzle to about 0.5 m. Since the sample is discharged at a high pressure to the spotted body by discharging at a rate of / s or more and spotted on the spotted body, the sample is spotted on the entire surface regardless of the deployment speed of the spotted body. , Spot coloring and uneven coloring can be reduced. The discharge speed is 1 to
It is preferably 2 m / s.
【0005】[0005]
【実施例】次に、本発明の実施例について図1ないし図
3を参照して説明する。図1において、11はノズル
で、図示しない駆動機構によって水平方向に移動すると
ともに、鉛直方向に上下動する。12はシリンジで、パ
ルスモータ13の駆動により、内蔵するピストン12a
が摺動可能になっている。また、このシリンジ12は、
チューブ14により、ノズル11および図示しない洗浄
水タンクに連通し、ノズル11側と洗浄水タンク側とは
三方弁15によって切り換えられる。Embodiments of the present invention will now be described with reference to FIGS. In FIG. 1, reference numeral 11 denotes a nozzle, which moves horizontally by a drive mechanism (not shown) and moves vertically in the vertical direction. Reference numeral 12 denotes a syringe, which is driven by a pulse motor 13 and has a built-in piston 12a.
Is slidable. In addition, this syringe 12
The tube 14 communicates with the nozzle 11 and a wash water tank (not shown), and the nozzle 11 side and the wash water tank side are switched by a three-way valve 15.
【0006】16は試験紙片の搬送装置で、パルスモー
タ17の正逆回転により、ローラ18,18間に巻装し
たベルト19を移動させる。これにより、このベルト1
9に固定した基台20が往復移動して、この基台20に
立設するピン21で試験紙片22を搬送する。この試験
紙片22は短冊状で、上面には所定間隔で多項目の被点
着体23が貼着されている。Reference numeral 16 is a test strip conveying device, which moves a belt 19 wound between rollers 18 by the forward and reverse rotations of a pulse motor 17. As a result, this belt 1
The base 20 fixed to 9 reciprocates, and the pin 21 standing on the base 20 conveys the test strip 22. The test paper piece 22 is in the form of a strip, and a plurality of items to be adhered 23 are attached to the upper surface at predetermined intervals.
【0007】24は制御装置で、予め記憶されたデータ
に基づいて前記両モータ13,17、三方弁15および
図示しないノズル駆動機構を制御する。この制御装置2
4には、試験紙片22の各被点着体23毎に必要な前記
ノズル11からの検体の吐出速度データおよび点着量デ
ータが記憶されている。A controller 24 controls the motors 13 and 17, the three-way valve 15 and a nozzle drive mechanism (not shown) based on previously stored data. This control device 2
In 4, the ejection speed data of the sample from the nozzle 11 and the spotting amount data required for each spotted body 23 of the test strip 22 are stored.
【0008】前記吐出速度は、シリンジ12内を摺動す
るピストン12aの移動速度、すなわち、パルスモータ
13への入力パルスの周波数に比例して決まる。この場
合、ノズル11から吐出される検体の吐出速度は0.5
m/s以上に設定する。ここで、本発明者らは、吸水性
能が悪く、展開速度が遅い潜血測定用試験紙の点着状態
(吸光度で判断する)を実験した。The discharge speed is determined in proportion to the moving speed of the piston 12a sliding in the syringe 12, that is, the frequency of the input pulse to the pulse motor 13. In this case, the ejection speed of the sample ejected from the nozzle 11 is 0.5.
Set to m / s or higher. Here, the present inventors conducted an experiment on the spotted state (judged by absorbance) of the occult blood measurement test paper, which has poor water absorption performance and a slow development speed.
【0009】ただし、前記展開速度は次のように決め
た。すなわち、各被点着体23を構成するものと同じ素
材の濾紙を短冊状に裁断する(40mm×250mm)。そ
して、この短冊を鉛直方向に吊した状態で先端部を浸し
(5mm)、所定時間(30分間)に吸い上げる高さを測
定する。このようにして測定された値は、被点着体用と
して処理されていない状態の濾紙の展開速度(160mm
/30min)と比較され、半分以下の値になれば、染み込み
速度が遅いと判断する。この場合、前記潜血測定用の被
点着体では51mm/30minであった。However, the developing speed was determined as follows. That is, a filter paper made of the same material as that of each spotted body 23 is cut into strips (40 mm × 250 mm). Then, the tip portion is dipped (5 mm) while the strip is suspended in the vertical direction, and the suction height is measured for a predetermined time (30 minutes). The value thus measured is the unfolding speed (160 mm) of the filter paper that has not been treated for the spotted object.
/ 30min), and if the value is less than half, it is judged that the soaking speed is slow. In this case, the spotted body for measuring the occult blood was 51 mm / 30 min.
【0010】本実験では、前記潜血測定用試験紙には、
青色1号色素1g/lを吐出速度2m/sで8μl点着
し、3mlの精製水に試験紙を入れて色素を溶出し、6
30nmの波長の光を照射して吸光度を測定する方法を
とった。以下、実験結果を示す。 実験例 1 2 3 4 5 6 7 8 9 10 吸光度 0.410 0.411 0.405 0.408 0.402 0.405 0.412 0.407 0.405 0.405 平均値 0.4070(ABS)、標準偏差 0.0032(%)、繰り返し精度 0.786(%)In the present experiment, the test paper for measuring occult blood was
8 μl of Blue No. 1 dye 1 g / l was ejected at a discharge speed of 2 m / s, and test paper was put into 3 ml of purified water to elute the dye.
The method of irradiating light with a wavelength of 30 nm and measuring the absorbance was used. The experimental results are shown below. Experimental example 1 2 3 4 5 6 7 7 8 9 10 Absorbance 0.410 0.411 0.405 0.408 0.402 0.405 0.412 0.407 0.405 0.405 Average 0.4070 (ABS), standard deviation 0.0032 (%), repeatability 0.786 (%)
【0011】このように、吐出速度を高速にすれば、所
望の吸光度を確実に得ることができ、検体がノズル11
の外周部に付着して点着量が少なくなるといった不具合
は発生しなかった。また、検体は前記被点着体23の全
面に均一に広がった。これは、検体が、被点着体23
に、その展開速度を越えて点着されるためであると考え
られる。As described above, by increasing the discharge speed, the desired absorbance can be reliably obtained, and the sample is discharged from the nozzle 11
There was no problem that the amount of spotting was reduced by adhering to the outer peripheral portion. In addition, the sample spread evenly over the entire surface of the adherend 23. This is because the sample is the pointed body 23
It is thought that this is because the spotting speed is exceeded and the spotting is performed.
【0012】一方、前記点着量は、シリンジ12内を摺
動するピストン12aの移動距離によって決定され、各
被点着体23毎に反射光量が精度良く測定できるように
設定されている。ただし、前記反射光量は、試験紙片2
2の各被点着体23に検体を点着した後、恒温機内で所
定時間恒温保持して呈色反応させ、所定波長の光を照射
した際の反射光を測定することにより反射光学濃度とし
て得られるものである。On the other hand, the spotted amount is determined by the moving distance of the piston 12a that slides in the syringe 12, and is set so that the amount of reflected light can be accurately measured for each spotted body 23. However, the amount of reflected light is 2
After spotting the sample on each of the spot-to-be-adhered bodies 23 of 2, the color reaction is carried out by keeping a constant temperature in the thermostat for a predetermined time, and the reflected light at the time of irradiating the light of the predetermined wavelength is measured to obtain the reflection optical density. Is what you get.
【0013】次に、前記点着装置による点着動作を説明
する。まず、三方弁15を切り換えて、ノズル11とシ
リンジ12とを連通する。そして、ノズル11を移動さ
せて、図示しない試験管等に挿入し、内部の検体を吸
引,保持する。続いて、前記ノズル11を点着位置に移
動させ、所定の高さに位置決めする。ここで、図示しな
いカセットに収納した試験紙片22を1枚、基台20に
設けたピン21によって一気に図1に示す最初の点着位
置まで搬送して停止する。この場合、ノズル11と被点
着体23との間隙寸法Cは、ノズル11からの検体の吐
出速度に対して、最大図2のグラフに示す関係となるよ
うに設定することが可能である。本実施例では、ノズル
11の内径Dが1.12mmであるのに対して間隙寸法
Cは0.6mmである。Next, the spotting operation by the spotting device will be described. First, the three-way valve 15 is switched to connect the nozzle 11 and the syringe 12. Then, the nozzle 11 is moved and inserted into a test tube (not shown) or the like to suck and hold the sample inside. Then, the nozzle 11 is moved to the spotting position and positioned at a predetermined height. Here, one piece of the test paper piece 22 housed in a cassette (not shown) is conveyed all at once by the pin 21 provided on the base 20 to the first spotting position shown in FIG. 1 and stopped. In this case, the gap dimension C between the nozzle 11 and the spotted body 23 can be set so as to have a maximum relationship with the ejection speed of the sample from the nozzle 11 as shown in the graph of FIG. In this embodiment, the inner diameter D of the nozzle 11 is 1.12 mm, while the gap dimension C is 0.6 mm.
【0014】そして、前記試験紙片22の被点着体23
a(潜血測定用)に、シリンジ12内に吸引してある尿
をノズル11から点着する。この点着は、前記制御装置
24から発せられる制御信号に基づいてパルスモータ1
3を駆動し、シリンジ12内のピストン12aの移動速
度および移動距離を制御することにより行う。具体的
に、この被点着体23aでは、吐出速度を2m/s、点
着量を8μlに設定する。Then, the pointed body 23 of the test paper piece 22
Urine sucked into the syringe 12 is spotted from the nozzle 11 to a (for occult blood measurement). This spotting is performed by the pulse motor 1 based on a control signal issued from the control device 24.
3 is driven to control the moving speed and moving distance of the piston 12a in the syringe 12. Specifically, for this spotted body 23a, the discharge speed is set to 2 m / s and the spotted amount is set to 8 μl.
【0015】続いて、前記試験紙片22を1ピッチ移動
させ、ノズル11の下方に被点着体23b(尿糖測定
用)を位置させて点着する。この場合、吐出速度を1m
/s、点着量を7μlに設定する。以下、他の被点着体
23に応じて前述の動作を繰り返す。Subsequently, the test paper piece 22 is moved by one pitch, and the spotted body 23b (for measuring urine sugar) is positioned below the nozzle 11 and spotted. In this case, discharge speed is 1m
/ S, and the spotted amount is set to 7 μl. Hereinafter, the above-described operation is repeated according to the other pointed body 23.
【0016】このようにして、試験紙片22の各被点着
体23に検体を点着し終わると、駆動機構によりノズル
11を移動させ、図示しない洗浄用試験管に挿入する。
そして、三方弁15をノズル11側から洗浄水タンク側
に切り換えて、ノズル11に洗浄水を供給し、洗浄す
る。その後、前述のように、ノズル11を移動させ、次
の検体が収容された試験管に挿入することにより、以下
同様な動作を繰り返す。前記各被点着体23に貼着され
た試験紙片22は次の工程に搬送され、従来同様、反射
光量が測定されることにより、各項目での異常の有無が
検出される。When the specimen has been spotted on each spotted body 23 of the test paper piece 22 in this manner, the nozzle 11 is moved by the drive mechanism and inserted into the washing test tube (not shown).
Then, the three-way valve 15 is switched from the nozzle 11 side to the cleaning water tank side, and cleaning water is supplied to the nozzle 11 for cleaning. After that, as described above, the nozzle 11 is moved and inserted into the test tube containing the next sample, and the same operation is repeated thereafter. The test paper piece 22 adhered to each of the spot-to-be-adhered bodies 23 is conveyed to the next step, and the amount of reflected light is measured in the same manner as in the prior art to detect the presence or absence of abnormality in each item.
【0017】以上のように、前記点着装置では、検体を
被点着体23での展開速度に拘わらず、常に高速で点着
できるので、点着時間が短く、点着位置の変更を短時間
で行なうことができ、検査効率が非常に良い。しかも、
検体は被点着体23の点着中心部での反応が進行し過ぎ
る前に全体に広がるので、点発色あるいは発色むらは発
生しなかった。As described above, in the spotting device, the specimen can be spotted at high speed regardless of the developing speed of the spotted body 23, so that the spotting time is short and the spotting position can be changed quickly. It can be done in time and the inspection efficiency is very good. Moreover,
Since the specimen spreads over the entire area before the reaction at the spotting center of the adherend 23 has proceeded too much, spot coloring or uneven coloring did not occur.
【0018】[0018]
【発明の効果】以上の説明から明らかなように、請求項
1記載の点着方法によれば、検体を高速で点着するの
で、点着時間を短縮でき、検査効率が向上する。しか
も、検体は短時間で被点着体全体に広がるので、点着中
心部の反応が進行し過ぎることがなく、点発色,発色む
ら等の不具合も発生せず、反射光量の測定精度を高める
ことができる。また、請求項2記載の点着方法のように
1〜2m/sで吐出すれば、展開速度の遅い被点着体で
は、請求項1記載の方法に比べてより発色むら等が発生
しにくくなる。As is apparent from the above description, according to the spotting method of the first aspect, the sample is spotted at a high speed, so that the spotting time can be shortened and the inspection efficiency is improved. Moreover, since the sample spreads over the entire spotted object in a short time, the reaction at the center of the spotting does not proceed too much, and problems such as spot coloring and uneven coloring do not occur, improving the measurement accuracy of the amount of reflected light. be able to. Further, when the discharge is performed at 1 to 2 m / s as in the spotting method according to claim 2, the spotted body having a slow developing speed is less likely to cause uneven coloring than the method according to claim 1. Become.
【図1】 本実施例に係る点着装置のブロック図であ
る。FIG. 1 is a block diagram of a spotting device according to an embodiment.
【図2】 本実施例に係る点着装置の吐出速度とノズル
と被点着体との間隙寸法との関係を示すグラフである。FIG. 2 is a graph showing the relationship between the discharge speed of the spotting device according to the present embodiment and the size of the gap between the nozzle and the spotted object.
【図3】 本実施例に係る点着状態を示す図である。FIG. 3 is a diagram showing a spotting state according to the present embodiment.
【図4】 従来例に係る点着装置の点着状態を示す図で
ある。FIG. 4 is a diagram showing a spotting state of a spotting device according to a conventional example.
11…ノズル、12…シリンジ、22…試験紙片、23
…被点着体。11 ... Nozzle, 12 ... Syringe, 22 ... Test strip, 23
… The pointed body.
Claims (2)
ズル内径の約2/3以下とし、前記ノズルから検体を約
0.5m/s以上で吐出して被点着体に点着することを
特徴とする検体の点着方法。1. A gap size from a nozzle to a spotted object is set to about 2/3 or less of an inner diameter of the nozzle, and a sample is discharged from the nozzle at about 0.5 m / s or more and spotted on the spotted object. A method for spotting a specimen, which is characterized in that
を特徴とする請求項1記載の検体の点着方法。2. The spotting method for a sample according to claim 1, wherein the discharge speed is 1 to 2 m / s.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP10957792A JP3051258B2 (en) | 1992-04-28 | 1992-04-28 | Sample spotting method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP10957792A JP3051258B2 (en) | 1992-04-28 | 1992-04-28 | Sample spotting method |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH05302931A true JPH05302931A (en) | 1993-11-16 |
JP3051258B2 JP3051258B2 (en) | 2000-06-12 |
Family
ID=14513795
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP10957792A Expired - Fee Related JP3051258B2 (en) | 1992-04-28 | 1992-04-28 | Sample spotting method |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP3051258B2 (en) |
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JP2002257837A (en) * | 2000-12-28 | 2002-09-11 | Eiken Chem Co Ltd | Method for controlling nozzle location for supplying urine sample by detecting location and thickness of reagent pad |
JP2006275697A (en) * | 2005-03-29 | 2006-10-12 | Techno Medica Co Ltd | Qualitative analyzer having automatic dispensing mechanism |
JP2011252725A (en) * | 2010-05-31 | 2011-12-15 | Sysmex Corp | Specimen analyzing device and specimen analyzing system |
JPWO2011152376A1 (en) * | 2010-05-31 | 2013-08-01 | アークレイ株式会社 | Analysis apparatus, analysis system, and analysis method |
JP2015505049A (en) * | 2011-12-16 | 2015-02-16 | シーメンス・ヘルスケア・ダイアグノスティックス・インコーポレーテッドSiemens Healthcare Diagnostics Inc. | Reagent card alignment apparatus and method |
JP5814919B2 (en) * | 2010-07-02 | 2015-11-17 | シスメックス株式会社 | Sample analyzer |
-
1992
- 1992-04-28 JP JP10957792A patent/JP3051258B2/en not_active Expired - Fee Related
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002257837A (en) * | 2000-12-28 | 2002-09-11 | Eiken Chem Co Ltd | Method for controlling nozzle location for supplying urine sample by detecting location and thickness of reagent pad |
JP2006275697A (en) * | 2005-03-29 | 2006-10-12 | Techno Medica Co Ltd | Qualitative analyzer having automatic dispensing mechanism |
JP4681919B2 (en) * | 2005-03-29 | 2011-05-11 | 株式会社テクノメデイカ | Qualitative analyzer with automatic dispensing mechanism |
JP2011252725A (en) * | 2010-05-31 | 2011-12-15 | Sysmex Corp | Specimen analyzing device and specimen analyzing system |
JPWO2011152376A1 (en) * | 2010-05-31 | 2013-08-01 | アークレイ株式会社 | Analysis apparatus, analysis system, and analysis method |
JP5917396B2 (en) * | 2010-05-31 | 2016-05-11 | アークレイ株式会社 | Analysis apparatus, analysis system, and analysis method |
JP5814919B2 (en) * | 2010-07-02 | 2015-11-17 | シスメックス株式会社 | Sample analyzer |
JP2015505049A (en) * | 2011-12-16 | 2015-02-16 | シーメンス・ヘルスケア・ダイアグノスティックス・インコーポレーテッドSiemens Healthcare Diagnostics Inc. | Reagent card alignment apparatus and method |
US10302572B2 (en) | 2011-12-16 | 2019-05-28 | Siemens Healthcare Diagnostics Inc. | Reagent card alignment system |
US10900910B2 (en) | 2011-12-16 | 2021-01-26 | Siemens Healthcare Diagnostics Inc. | Reagent card alignment method for sample dispensing |
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