JPH05279700A - Detergent composition - Google Patents

Abstract

PURPOSE:To provide a detergent composition comprising a nonionic surfactant, a neutral protease, etc., a metal ion-sequestering agent, a benzotriazole, etc., in specific amounts, excellent in cleaning endoscopes and not injuring their materials. CONSTITUTION:The objective composition comprises 20-33 pts.wt. of a nonionic surfactant (e.g. a polyoxyethylene alkyl or alkenyl ether containing a 10-20C alkyl or alkenyl group and 1-20 moles of the addition-reacted ethylene oxide), (B) 20-33 pts.wt. of a neutral and/or alkaline protease having an activity of 10000-60000Au/g, (C) 20-33.5 pts.wt. of a metal ion-sequestering agent (e.g. nitrilotriacetic acid salt or citric acid), and (D) 0.05-0.5 pt.wt. of a benzotriazole or its amine salt, the composition giving a pH of 7-8 when dissolved in water.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cleaning composition which is excellent in cleaning endoscopes.

[0002]

2. Description of the Related Art In recent years, the use of endoscopes has been rapidly increasing in medical diagnosis or treatment, and one endoscope is used to diagnose and treat many patients. The endoscope used in this way is significantly contaminated by body fluids, blood, etc. in the body cavity, but the endoscope is made of various materials and has complicated and delicate instruments having pores and movable parts. Therefore, a large amount of labor and time are required for the cleaning, and as a result, the usage rate of the patient for one endoscope is restricted.

As a cleaning method after using the endoscope in such a situation, cleaning with water or cleaning with a cleaning machine dedicated to the endoscope is performed. However, since the cleaning of the pores is insufficient in all cases, the residue of stains is denatured and solidified by sterilization and disinfection in the subsequent process, which deteriorates the movement of moving parts over time and causes pore clogging. Or In addition, a washing machine dedicated to an endoscope is very expensive. On the other hand, with a cleaning agent used for cleaning ordinary medical instruments / equipment, not only is the cleaning of the pore portion of the endoscope insufficient, but there is also the risk of damaging the metal or rubber portion.

On the other hand, the method for cleaning and sterilizing an endoscope and its preparation disclosed in JP-A-63-135123 include a low-foaming nonionic surfactant 0.1 to 1.0 g / l and a proteolytic enzyme 0.03 to 0.3 Au. (Anson unit) / l and complexing agent 0.03-0.3g / l, pH
Although a cleaning solution consisting of 6 to 8 is disclosed, the cleaning solution shown here does not damage the material of the endoscope, but is not sufficient to completely remove the dirt in the pores. there were. Therefore, it has been desired to develop a cleaning agent exclusively for endoscopes.

[0005]

SUMMARY OF THE INVENTION Accordingly, an object of the present invention is to provide a cleaning composition which has sufficient cleaning properties even in the pores of an endoscope without damaging its material in cleaning the endoscope. Is.

[0006]

Means for Solving the Problems The inventors of the present invention have achieved the present invention as a result of intensive research to solve the above problems.
This detergent composition comprises (a) a nonionic surfactant: 20 to 33.
Parts by weight, (b) neutral and / or alkaline protease: 20-33 parts by weight, (c) sequestering agent: 20-33.5 parts by weight and (d) benzotriazole or its amine salt: 0.05-0.5 parts by weight Becomes the above in this composition
(b) A neutral and / or alkaline protease having an activity of 10,000 to 60,000 Au (Anson unit) / g, and having a pH of 7 to 8 when the composition is dissolved in water Is.

The present invention will be described in detail below. The nonionic surfactant which is the component (a) of the cleaning composition of the present invention,
Conventionally known, for example, (1) average carbon number 10 ~
Polyoxyethylene alkyl or alkenyl ether having 20 alkyl or alkenyl groups and having 1 to 20 mol of ethylene oxide added, (2) average carbon number 6 to 12
Having an alkyl group of 1 to 20 moles of ethylene oxide added thereto, and further (3) polyoxypropylene alkyl ether, polyoxyethylene polyoxypropylene alkyl ether, higher fatty acid glycerin ester, higher Examples thereof include fatty acid glycol ester, higher fatty acid sorbitan ester, higher fatty acid ethylene oxide adduct, sucrose fatty acid ester, and alkyl saccharide.

Specific commercially available products of these surfactants and their manufacturing companies include "Nonion", "Pronone" (NOF Corporation), "Emulgen" (Kao), "Neugen" (Daiichi Kogyo Seiyaku), Examples include "Triton" (Rohm and Haas, USA), "Tween" (ICI Americas), "Sterox" (Monsanto Kagaku, USA) and the like, which are used alone or in combination of two or more kinds.

The proportion of this nonionic surfactant in the detergent composition is 20 to 33 parts by weight, preferably 22 to 30 parts by weight. If this amount is less than 20 parts by weight, favorable detergency cannot be obtained, and if it exceeds 33 parts by weight, foaming of the washing solution becomes too high, which may affect the stability of neutral and / or alkaline proteases as component (b). become.

As the neutral or alkaline protease as the component (b), conventionally known ones can be used.
llus sulitilis, Bacillus licheniformis, Bacillus s
Proteases obtained from specific bacterial cells such as p. Specific commercial products and their manufacturing companies include "Alcalase", "Esperase" and "Sabinase" (Novo, Denmark), "Bioplase" (Nagase Biochemical Industry), "GR Protease" (USA,
Monsanto), "API-21" (Showa Denko), etc.,
These are used alone or in combination of two or more.

The blending ratio of this component in the present cleaning composition is
20 to 33 parts by weight, particularly 22 to 30 parts by weight are preferred. The activity of the protease contained in 1 g of the composition is 10,000 to 60,000 Au (Anson unit) / g, and particularly preferably 20,000 to 50,000 Au (Anson unit) / g. When the blending amount and the activity of the protease are out of the above ranges, preferable detergency cannot be obtained.

Examples of the sequestering agent as the component (c) include nitrilotriacetic acid salt, ethylenediaminetetraacetic acid salt, diethylenetriaminepentaacetic acid salt; citric acid, gluconic acid, glycolic acid, malic acid, tartaric acid, lactic acid and the like. Oxycarboxylic acids or salts thereof; polyacrylic acid salts, hydroxypolyacrylic acid salts, polyacetal carboxylates, salts of copolymers of acrylic acid and maleic anhydride,
Examples thereof include polymer electrolytes such as a salt of a copolymer of maleic anhydride and an olefin, a salt of a copolymer of acrylic acid and methacrylic acid, and these are used alone or in combination of two or more kinds. The mixing ratio of this component in the present cleaning composition is 20 to 33.5 parts by weight, particularly 22 to 30 parts by weight is preferable, and if it is out of this range, preferable detergency cannot be obtained.

Specific commercial products of benzotriazole or its amine salt as component (d) and the names of its manufacturing companies include "CVI" and "CVI Liquid" (Cilest, USA), "Pulventol CI8-100" ( Germany, Bayer) and the like, which are used alone or in combination of two or more kinds.

The blending ratio of this component in the detergent composition is
0.05 to 0.5 parts by weight, particularly 0.1 to 0.3 parts by weight are preferred. If this amount is less than 0.05 parts by weight, an excellent rust preventive effect on the metal material of the endoscope cannot be obtained, resulting in damage.
Further, even if added in excess of 0.5 parts by weight, further improvement of the effect cannot be expected, and only the cost is increased.

In addition to the above-mentioned components, the detergent composition of the present invention may optionally contain enzymes other than proteases such as lipase, amylase and cellulase; lower alcohols,
Hydrotropes such as polyhydric alcohols and lower allyl sulfonic acids; anti-redeposition agents such as carboxymethyl cellulose and polyethylene glycol; enzyme stabilizers such as boric acid, boron oxide or alkali metal borates; other foam regulators, fragrances, etc. Can be added within a range that does not impair the object of the present invention.

The detergent composition is usually used by dissolving it in water, and the concentration in this case is 0.01 to 5%, especially 0.03 to
It is preferably a 3% aqueous solution. The pH of the aqueous solution at that time is 7 to 8, and particularly preferably 7.3 to 7.8. If this is less than 7, good detergency cannot be obtained,
On the other hand, if it exceeds 8, the material of the endoscope is damaged. An organic acid such as citric acid is used as a pH adjuster for this purpose.

[0017]

EXAMPLES The present invention will be described below with reference to Examples and Comparative Examples, but the present invention is not limited to these descriptions. Example 1. Water was added to each component (parts by weight) shown in Table 1 and Table 2 to prepare a 1% aqueous solution of the cleaning composition, and a cleaning test and a metal damage test were conducted by the following methods, and the results are shown in Table 1 and Table. It is also shown in 2. The pH of each cleaning solution was adjusted with citric acid.

Washing test method: One end of a silicon tube having an inner diameter of 1 mm, an outer diameter of 2 mm and a length of 1 m was tightly stoppered, bovine blood was placed in the inside thereof, and air-dried for one week or more. Cut this into 10 cm lengths and put them in 100 ml of the 1% cleaning solution in Table 1 one by one 15
Washed for minutes. Then, it was immersed in 100 ml of pure water twice for 3 minutes and air dried. The protein adhering to the tube before and after washing was extracted by shaking with 100 ml of 0.1N sodium hydroxide aqueous solution (100 times / min, 90 ° C, 2 hours), and the sample was copper-
The Folin reagent was used for color development, the absorbance (750 nm) was measured, and the washing rate was calculated by the following formula, which was used as the evaluation standard. In addition,
R _S and R _W are the absorbances of the sample before and after washing, respectively. Detergency ratio _{(%) = [(R S} -R W) / R S] × 100

Metal damage test method: zinc plate, brass plate,
Each of a copper plate and an aluminum plate cut into a 2 cm square was immersed in a 1% aqueous solution of each detergent composition shown in Table 1 at room temperature for 24 hours, and the degree of corrosion was visually evaluated according to the following criteria. A: No corrosion at all, B: Some corrosion, C: Very corrosion.

[0020]

[Table 1]

[0021]

[Table 2]

Example 2. Upper gastrointestinal endoscope OLYMPUS G
Experiment with IF (manufactured by Olympus Optical Co., Ltd.) in Example 1 above
It was immersed in a 1% aqueous solution of the No. 1 detergent composition for 30 minutes at room temperature for one day, followed by rinsing and air drying. This was repeated for 5 months, but no damage was found on the endoscope.

[0023]

The cleaning composition of the present invention does not damage the material of the endoscope and can remove the dirt adhering to the pores of the endoscope within a short time. It is effective for improvement of cleanliness and maintenance, and efficient diagnosis or treatment is possible, which is significant for medical treatment.

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Claims (1)

[Claims] 1. A nonionic surfactant: 20 to 33 parts by weight,
(b) Neutral and / or alkaline protease: 20-
33 parts by weight, (c) sequestering agent: 20 to 33.5 parts by weight and (d) benzotriazole or its amine salt: 0.05 to
0.5 parts by weight, and the activity of the above-mentioned (b) neutral and / or alkaline protease in this composition is 10,000 to 6
A cleaning composition, characterized in that it has a pH of 7 to 8 when it is dissolved in water, and is 0.00000 Au (Anson unit) / g.