JPH05246846A - Composition for promoting digestion of protein - Google Patents

Abstract

PURPOSE:To provide the subject composition useful for promoting human health by increasing the concentration of cholecystokinin in blood to promote the secretion of pancreatase and increasing the activity of proteinase in the intestines to promote the digestion and absorption of protein. CONSTITUTION:The objective composition contains one or plural compounds selected from polyphenol compounds consisting of (+)-catechin, (+)-gallocatechin, (+)-gallocatechin gallate, (-)-epicatechin, (-)-epicatechin gallate, (-)- epigallocatechin and (-)-epigallocatechin gallate.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to a protein digestion promoting composition. More specifically, increasing the concentration of cholecystokinin in the blood promotes the secretion of digestive enzymes from the pancreas, and increases the activity of proteolytic enzymes in the digestive tract to help the digestion of proteins. The present invention relates to a protein digestion promoting composition which is effective for

[0002]

2. Description of the Related Art Production of digestive enzymes in pancreas, pancreatic enzymes,
Cholecystokinin is known as a hormone that promotes the secretion of bile. Various proteins are known as substances that elevate cholecystokinin in blood, and it is said that cholecystokinin is released into the bloodstream through receptors present in cells of the gastrointestinal wall. There is. on the other hand,
Among proteins, various trypsin inhibitors also increase the blood concentration of cholecystokinin, which is reported to be a feedback effect for inhibiting the action of trypsin [ADVANCE IN EXPERIMENTAL
MEDICINE AND BIOLOGY, Vol.199, PP123-132 (1986)].

Chymotrypsinogen, trypsinogen, and carboxypeptidase are known as proteolytic enzymes secreted as pancreatic juice or precursors thereof, and chymotrypsinogen and trypsinogen are activated to chymotrypsin and trypsin to decompose proteins. Is known to do. The ingested protein is decomposed into constituent amino acids by these proteolytic enzymes as well as various proteolytic enzymes secreted from the duodenum, and is absorbed from the intestine to serve as a nutrient source. On the other hand, if gastric acid, pancreatic enzymes, and bile secretion decrease,
The environment of the digestive tract for lowering the molecular weight of food and insufficient digestive enzymes cause indigestion, and nutrients are not absorbed from the intestinal tract, resulting in a deficiency of nutritional sources.

Regarding the effect of polyphenol compounds on digestive enzymes, it has been found that polyphenols have an action of inhibiting the activity of α-amylase secreted in saliva and pancreatic juice, and tea polyphenol is an active ingredient. A patent application has been filed as an α-amylase activity inhibitor (JP-A-3-133928).

[0005]

To increase the concentration of cholecystokinin in the blood to promote the synthesis and secretion of digestive enzymes in the pancreas, and to increase the activity of various enzymes in the digestive tract, Since it contributes to the promotion of digestion of food, and the nutrients produced thereby contribute to the formation of a healthy body, in the pancreas by increasing the level of cholecystokinin in the blood when necessary, that is, after eating. It is important to promote the synthesis and secretion of digestive enzymes and further increase the activity of digestive enzymes in the digestive tract. An object of the present invention is to provide a composition for promoting digestion of proteins, in particular.

[0006]

Means for Solving the Problems The present inventor has conducted extensive studies as an index of the concentration of cholecystokinin in blood and the activity of proteolytic enzymes in the digestive tract, and as a result, plants of the Camellia family, particularly It was found that a polyphenol compound contained in tea used for daily drinking increases the concentration of cholecystokinin in blood and further increases the activity of proteolytic enzymes in the digestive tract, and completed the present invention. ..

The polyphenols of the present invention are (+)-
Catechin, (+)-gallocatechin, (+)-gallocatechin gallate, (−)-epicatechin, (−)-epicatechin gallate, (−)-epicatechin and (−)-epigallocatechin gallate Refers to a polyphenol compound.

The polyphenol compound used in the present invention can be obtained from a water or alcohol extract of tea and an ethyl acetate-soluble fraction of the extract, but other sources and chemically synthesized products may also be used.

A typical method for preparing the polyphenol compound of the present invention is as follows. First, tea is extracted at room temperature with a sufficient amount of water or alcohol. After extraction, the residue is separated by a known method to obtain an extract. By removing the solvent from the extract, a powder having a polyphenol purity of 35 to 45% can be obtained, and can be used as it is in the industry. However, in order to further increase the purity of the polyphenol compound, hexane, chloroform and ethyl acetate are sequentially used for partitioning to obtain an ethyl acetate-soluble fraction. A powder having a polyphenol purity of 70 to 80% can be obtained by removing ethyl acetate from the extract according to a conventional method.

The alcohol used for extraction is preferably a lower alcohol such as methyl alcohol, ethyl alcohol, n-propyl alcohol, isopropyl alcohol or butyl alcohol in view of operability and extraction efficiency.

Further, the ethyl acetate-soluble fraction obtained above was subjected to silica gel chromatography to obtain chloroform-methyl alcohol (20: 1, V / V) and chloroform-methyl alcohol (10: 1, V / V). Seven kinds of compounds can be obtained by sequentially eluting with the solvent. In addition, if necessary, Sephadex LH-20
7 of higher purity can be obtained by eluting with a suitable solvent such as methyl alcohol, or by using a recycle HPLC (manufactured by Nippon Analytical Industry Co., LC-908, GS-320 column, solvent methyl alcohol). The compound can be obtained.

When these obtained polyphenol compounds are used in the present invention, they can be used alone, as a mixture of two or more kinds, or even a crude extract containing polyphenol. The product of the present invention exerts its effect by oral ingestion.
The form of use can be tablets, granules, capsules, syrups, drinks or various foods.
The human dose of the product of the present invention is preferably 0.001 g to 0.06 g / kg body weight per day as a polyphenol compound,
The dose is less than 0.001g / kg body weight, the effect is weak, 0.5g
If the body weight is more than kg, unfavorable effects such as inactivation of digestive enzymes in the intestinal tract occur, which is not preferable.

[0013]

[Action] It is not clear what action the polyphenol compound, which is the active ingredient of the present invention, raises the concentration of cholecystokinin in blood, and further the activity of proteolytic enzyme in the intestinal tract. As a factor that increases the activity of proteolytic enzymes of the, polyphenol compounds stimulate the receptors of cholecystokinin-releasing cells present in the intestinal tract and release cholecystokinin in the blood, and this cholecystokinin is digested from the pancreas. It is presumed that it is due to promoting the secretion of the enzyme.
Hereinafter, the details will be described with reference to Examples and Test Examples.

[0014]

【Example】

Example 1 About 15 liters of water was added to 1 kg of commercial green tea, and the mixture was stirred at 80 ° C. for 3 days.
Time extracted. The extract obtained by filtration was concentrated to dryness to obtain 350 g of hot water extract of green tea (purity 38% as a mixture of polyphenol compounds).

Example 2 To 350 g of the hot water extract obtained in Example 1, 8 liters of water was added and dissolved, and the mixture was sequentially distributed with hexane and chloroform. Ethyl acetate (10 liters) was added to the aqueous layer after partitioning, and the mixture was vigorously stirred and stood still, then the ethyl acetate layer was separated, the ethyl acetate was removed, and the fraction was dried to obtain 70 g of an ethyl acetate-soluble fraction. The purity of all polyphenol compounds in the ethyl acetate-soluble fraction was 7
4.5%, and the proportion of the polyphenol compound was (+)
-Catechin 3.5%, (+)-gallocatechin 14.8%,
(+)-Gallocatechin gallate 11.6%, (-)-epicatechin 7%, (-)-epicatechin gallate 4.6%,
(−)-Epigallocatechin gallate 15.0% and (−)-epigallocatechin gallate 18.0%.

Example 3 10 g of the ethyl acetate-soluble fraction obtained in Example 2 was subjected to silica gel chromatography (solvent, chloroform-methyl alcohol, 20: 1, 10: 1, V / V), Sephadex LH-. 20 (solvent; methyl alcohol), recycled H
PLC (Nippon Analytical Industry Co., Ltd. LC-908, GS-320)
(+)-Catechin 0.3 g, (+)-gallocatechin 1.22 g, (+)-gallocatechin gallate 0.9 g, by sequentially using a column and a solvent; methyl alcohol)
0.5 g of (-)-epicatechin, 0.38 g of (-)-epicatechin gallate, 1.2 g of (-)-epigallocatechin gallate, and 1.5 g of (-)-epigallocatechin gallate were obtained.

Example 4 50 g of the ethyl acetate-soluble fraction obtained in Example 2 was sterilized by heating, and then hydroxypropylmethylcellulose was used as an excipient.
450 g and 10 g of stearic acid as a lubricant were added and compressed to obtain 200 tablets.

Example 5 50 g of the ethyl acetate-soluble fraction obtained in Example 2 was sterilized by heating, and then 0.4 g per capsule was filled in the Japanese Pharmacy Capsule (# 1) to obtain 100 capsules.

Test Example 1 AIN feed (50% sucrose, 20% casein, 0.3 DL methionine, 15% corn starch, 5% corn oil) containing 0.1% or 0.5% of the polyphenol compound obtained in Example 2 Cellulose 5%, mineral mix 3.5
%, Vitamin mix 1%, choline bicarbonate 0.2%), 3.5g of which was mixed with water and administered into the stomach of 5-week-old Wistar male rats, and the rats were sacrificed over time. ,
Blood and small intestine contents were collected. The concentration of cholecystokinin in blood was quantified by a radioimmunoassay method using an anti-cholecystokinin antibody, and the concentration of trypsin in the small intestinal contents was produced by using Tosyl arginine methyl ester as a substrate. Arginine was quantified by a colorimetric method. AIN feed was used as a control feed.
The value at each point is shown as the average value of the values obtained from 5 rats.

[0020]

[Table 1]

As is clear from Table 1, the polyphenol compound significantly increased the concentration of cholecystokinin in blood as compared with the control.

[0022]

[Table 2]

As is clear from Table 2, the polyphenol compound significantly increased the activity of trypsin in the intestinal tract as compared with the control.

[0024]

INDUSTRIAL APPLICABILITY The polyphenol compound, which is the active ingredient of the present invention, increases the concentration of cholecystokinin in blood, thereby promoting the secretion of pancreatic enzyme from the pancreas, and the activity of proteolytic enzyme in the intestinal tract. Since it can be increased, it is extremely effective in promoting digestion of proteins. Since this component is a component of tea that has been used for drinking since ancient times, its safety is extremely high and it can be supplied in large amounts. Therefore, the present invention provides enhancement of human physical strength and health. It is a great contribution to promotion.

 ─────────────────────────────────────────────────── ─── Continuation of front page (72) Inventor Yu Fujiki 9-5 Akahori Shinmachi, Yokkaichi-shi, Mie Taiyo Kagaku Co., Ltd. (72) Inventor Kim Takehisa 9-5 Akahori-shinmachi, Yokkaichi, Mie Prefecture Gaku Co., Ltd.

Claims (1)

[Claims] (1) (+)-catechin, (+)-gallocatechin, (+)-gallocatechin gallate, (-)-epicatechin, (-)-epicatechin gallate, (-)-epigallocatechin and (-) ) -A protein digestion promoting composition comprising one or more compounds selected from the group of polyphenol compounds consisting of epigallocatechin gallate.