JPH05130837A - Pet food - Google Patents

Abstract

PURPOSE:To obtain a pet food useful for health preventing yeast decomposition with an alkali phosphatase, maintaining solubilizing effects on mineral by adding carrageenan, etc., to a pet food containing casein phosphopeptide. CONSTITUTION:A pet food containing casein phosphopeptide(CPP) is blended with preferably 0.1-10 pts.wt. (based on 1 pt.wt. CPP) of one or more selected from carrageenan, pectin, dextran sulfate and mucin to give the objective pet food. The food is preferably enriched with calcium and/or iron.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a pet food containing casein phosphopeptide (CPP). More specifically, the present invention relates to a CPP-containing pet food containing one or more selected from the group consisting of carrageenan, pectin, dextran sulfate and mucin.

[0002]

2. Description of the Related Art In recent years, with the spread of pet food in the pet industry, the lifespan of pets has been extended, and as a result, diseases such as osteomalacia and osteoporosis, which are thought to be due to a decrease in the availability of calcium over time, have become common. The number of pets is also increasing. In addition, the proportion of pets kept indoors is increasing, and due to the lack of exercise and lack of solar radiation, the synthesis of vitamin D ₃ is impaired, which causes a decrease in calcium availability. Is becoming

On the other hand, in pet foods, corn, wheat, etc. as carbohydrates, protein as meat meal, fish meal, defatted soybeans, etc. as fat,
As animal / vegetable fat and calcium, calcium carbonate, calcium phosphate, etc. are generally blended. In order to improve the utilization of calcium, the amount of calcium blended is increased or the amount of calcium salt supplied is increased. It was common to increase the amount of calcium absorbed depending on the form.

However, calcium over-feeding is
Decreased protein availability (Goto, “Nutrition and Food” VOL
32 1 to 11 (1979)), or a decrease in the availability of other minerals (Naito, supra) has been reported.
CPP is a peptide with a high phosphorylated serine content obtained by reacting milk casein with a proteolytic enzyme (Naito, "Journal of Japan Society of Nutrition and Food Science" VOL. 39, 433-439).
(1986)). This CPP has a solubilizing effect on minerals such as calcium and iron, and is attracting attention as a substance that promotes absorption of these minerals, and is widely used in the field of food.

[0005] In poultry feed, there is also a report that the absorption rate of calcium is improved by the interaction between CPP and calcium. (Japanese Patent Laid-Open No. 1-285158) However, it is presumed that CPP is easily dephosphorylated by alkaline phosphatase present in the mucosa of the small intestine, and the present inventors have actually confirmed the degradation (see Test Example). ). Furthermore, it has been known that CPP dephosphorylated by a kind of phosphatase completely disappears the mineral solubilizing effect (Saito, "Japan Food Science" VOL 29. 21-32 (1990)). However, a method for preventing such enzymatic degradation has not yet been known.

[0006]

Therefore, the present invention is a CP
An object of the present invention is to provide a pet food containing P, in which the above-mentioned enzymatic decomposition is prevented and the mineral solubilizing effect of CPP is maintained.

[0007]

[Means for Solving the Problems] The present inventors have investigated CPP by alkaline phosphatase present in the mucosa of the small intestine.
As a result of research to find a substance capable of preventing the dephosphorylation of Escherichia coli, it was found that carrageenan, pectin, dextran sulfate, and mucin, which are commonly used as thickeners, are effective, and the present invention was completed.

That is, the present invention relates to a CPP-containing pet food, which comprises one or more selected from the group consisting of carrageenan, pectin, dextran sulfate and mucin. Generally, carrageenan is a mixture of several components, κ-carrageenan, λ
-There are components called carrageenan, i-carrageenan, and mu-carrageenan, and any of these components may be used in the present invention.

Carrageenan is preferably used in pet food containing CPP in an amount of about 0.001 to 50 times the weight of CPP.
It is preferable to use 10 times the weight. If the amount of carrageenan used is less than 0.001 times the weight of CPP, the effect of using carrageenan cannot be expected, and 50
If it is more than double weight, the viscosity of carrageenan adversely affects the physical properties of the pet food, and the raw material cost becomes too high, which is not preferable.

The pectin used in the present invention is widely contained in plant tissues, and generally, citrus fruits (especially lime, lemon, orange, grapefruit, etc.) and apple pomace are used as raw materials. ..
Pectin is C in pet foods containing CPP
It is preferable to use about 0.001 to 50 times the weight of PP, and it is particularly preferable to use the weight of 0.1 to 10 times the CPP. The amount of pectin used is 0 relative to CPP.
If it is less than 001 times the weight, the effect of using pectin cannot be expected so much, and if it is more than 50 times the weight, the physical properties of pet food are unfavorably affected.

The dextran sulfate used in the present invention is a sulfate ester of dextran, and 0.001 to CPP of pet food containing CPP is used.
It is preferable to use about 50 times the weight, but it is particularly preferable to use 0.1 to 10 times the weight of CPP. If the amount of dextran sulfate used is less than 0.001 times the weight of CPP, the effect of using dextran sulfate cannot be expected, and if it is more than 50 times the weight, it is not economical.

The mucin used in the present invention is an animal viscous protein (glycoprotein) that is precipitated as a viscous coagulum (mucin coagulum) by a dilute inorganic acid and is not dissolved by the addition of excess acid. There are mucins and egg white mucins. Mucin is preferably used in pet food containing CPP in an amount of about 0.01 to 50 times the weight of CPP, and particularly preferably about 0.1 to 10 times the weight of CPP. When the amount of mucin used is less than 0.01 times the weight of CPP, the effect of using the mucin cannot be expected, and when it exceeds 50 times the weight, it is not economical.

In the present invention, the above carrageenan / pectin / dextran sulfate and mucin can be used alone or in combination of two or more. Generally, pet food has a dry food content of about 10% and a water content of 30%.
There are some semi-moist foods and canned foods having a water content of about 75%, but the present invention is not limited to these.

The pet food itself contains some calcium, but calcium can also be fortified. In that case, the calcium source is not particularly defined and is not related to its origin. Further, the amount of calcium to be fortified and added is not particularly limited, and may be within a range required from the nutritional aspect of general pets.

[0015]

INDUSTRIAL APPLICABILITY According to the present invention, in pet food containing CPP, CPP is not easily dephosphorylated by alkaline phosphatase existing in the mucosa of the small intestine, and the mineral solubilizing action of CPP is exerted. Pet food is provided that is maintained.

[0016]

EXAMPLES The present invention will be described in more detail by way of test examples and examples. Test Example 1 Degradation test of CPP by alkaline phosphatase As CPP, "CPP-III" manufactured by Meiji Seika Co., Ltd. was used. The same applies to the following test examples and examples.

CPP (1 mg / ml) was dissolved in 55 mM Tris-hydrochloric acid buffer containing 5.5 mM magnesium chloride, calf intestine-derived alkaline phosphatase (manufactured by Sigma) was added, and the mixture was kept at 37 ° C. for 20 minutes. For the degradation of CPP, the phosphate dephosphorylated by phosphatase was measured for its absorbance at 660 nm by a modified method of Fiskesabarov (Ogawara, Uchino, "J. Antibiotics", VOL. 38,
153-156 (1985). As is clear from the results shown in Table 1 below, an increase in phosphoric acid due to the decomposition of CPP was observed.

Table 1 Alkaline phosphatase addition amount Phosphoric acid production amount Enzyme amount (unit) Absorbance 660 nm 0 0 0.2 0.605 0.6 0.6 0.911 1.0 1.106 1.5 1.230 3.0 1 .238

From this test, it is easily understood that CPP is enzymatically degraded in the small intestine and loses its effect. Test Example 2 Effect of Carrageenan, Pectin, Dextran Sulfate and Mucin to Prevent CPP Degradation When various samples of various concentrations were added in the method of Test Example 1, as shown in Table 2 below, CPP was degraded by alkaline phosphatase. Could be prevented. Among the carrageenans, λ-carrageenan was particularly effective.

[0020]

Test Example 3 In Test Example 2, it was discovered that each sample inhibits the degradation of CPP by the intestinal degrading enzyme. Therefore, regarding other functions of CPP, specifically, the inhibitory effect of CPP on calcium phosphate precipitation ( Naito, "Journal of Japan Society of Nutrition and Food Science" VOL. 3
9, 433-439 (1986)).

It was tested whether carrageenan or the like retains the calcium phosphate precipitation inhibiting effect under the condition that CPP is decomposed by an enzyme and the calcium phosphate precipitation inhibiting effect is lost.

1. Reagents (1) 10 mM calcium solution 10 mM CaCl ₂ physiological saline (2) 20 mM phosphoric acid solution 20 mM Na ₂ HPO ₄ physiological saline (3) CPP-III Meiji Seika Co., Ltd. (4) Carrageenan (λ); Carrageenan (κ); Pectin (Lemon, manufactured by Wako Pure Chemical Industries, Ltd.); Pectin (low methoxyl type (LM), manufactured by Sanei Chemical Industry Co., Ltd.); Dextran sulfate (Mw: ~ 500) 1,000, Falmatia
Japan Co., Ltd.); mucin (porcine stomach; Wako Pure Chemical Industries, Ltd.) (5) Alkaline phosphatase

2. Method 6 mg of CPP of (3), each sample of (4), 20 units of enzyme of (5) and 10 ml of calcium solution of (1),
10 ml of the phosphoric acid solution of (2) was added and heated at 37 ° C. for 1 hour. Then, the reaction solution was centrifuged at 2,500 rpm for 5 minutes to collect the supernatant. As is clear from the results shown in Table 3 below, each sample had a calcium phosphate precipitation inhibiting effect.

Under the condition that 4.7 mM of calcium is solubilized by CPP, the intestinal degrading enzyme loses the function of CPP and the solubilization is reduced to 1.5 mM.
Carrageenan (λ) is 20μ for P300μg / ml
Effective above g / ml, carrageenan (κ) is 100 μg
It was effective at more than / ml. Also, CPP 300μg / ml
In contrast, pectin (LM: low methoxyl type) is 20
100 μg / ml for pectin made of lemon above μg / ml
Effective above ml. In addition, mucin and dextran sulfate were effective at 200 μg / ml or more.

Table 3 ─────────────────────────────────── Sample name Concentration ALP Ca concentration Inhibition rate μg / ml u / ml mM (%) ─────────────────────────────────── Control, physiological saline 0 0 4.7-control, saline 0 1 1.5-carrageenan (λ) 200 1 4.7 100 100 100 1 4.7 100 40 1 4.5 94 20 1 4.5 945 10 1 1.250 Carrageenan (κ) 200 1 4.5 94 100 1 3.9 73 340 1 1.7 6 20 1 1.30 0 ─────────────────────── ───────────── Pectin (LM) 200 1 4.7 100 100 100 1 4.7 100 40 40 1 4.5 94 20 1 4.5 93 10 10 1.25 0 Pectin 200 1 4.5 94 (made of lemon 100 1 4.4 91 40 1 1.6 3 3 20 1 1.7 6 ────────────────────────────────── ─── Dextran sulfate 400 1 4.7 100 300 1 4.5 94 200 1 1.45 92 100 1 1.35 0 ────────────────────── ────────────── Muchin 400 1 4.7 100 100 300 1 4.6 97 200 200 1 4.55 95 100 1 1.35 0 ───────── ───────────────────────────

Example 1 Corn 33 parts Wheat flour 30 Defatted soybean 15 Meatmeal 10 Chicken meal 5 Bran 5 Vitamin / mineral mix 1 CPP 0.5 Carrageenan 0.5 A composition comprising the above ingredients was mixed and crushed to form a 1 mm opening. The size was adjusted using a sieve having the same. Water was added to the obtained composition to about 27% by weight, and a cooking extruder (made by Wenger) was equipped with a round die having a diameter of 8 mm.
Extrusion treatment was performed to obtain a swelled product. The obtained puffed product was dried with a belt dryer at 120 ° C. for 20 minutes, and then 5 parts of soybean oil was sprayed on 95 parts of the puffed product to obtain a dry dog food.

Example 2 Corn 32 parts Wheat flour 30 Defatted soybean 15 Meat meal 10 Chicken meal 5 Bran 5 Vitamin / mineral mix 1 Calcium phosphate 1 CPP 0.5 Carrageenan 0.5

The composition consisting of the above components is mixed and crushed 1
The particles were sized using a sieve having an opening of mm. The obtained composition was hydrolyzed to about 27% by weight, and a cooking extruder (manufactured by Wenger Co.) was equipped with a round die having a diameter of 8 mm and extruded to obtain a swelled product. The obtained puffed product was dried with a belt dryer at 120 ° C. for 20 minutes, and then 5 parts of soybean oil was sprayed on 95 parts of the puffed product to obtain a dry dog food.

Example 3 Corn 32 parts Wheat flour 25 Defatted soybean 15 Fish meal 10 Meat meal 10 Chicken meal 5 Vitamin / mineral mix 1 Calcium phosphate 1 CPP 0.5 Pectin 0.5 A composition comprising the above ingredients was prepared as an example. 4 as well as 1
A round die of mm was attached to obtain a puffed product.

To 92 parts of the expanded product, 5 parts of soybean oil and 3 parts of fish extract were sprayed to obtain a dry cat food.

Example 4 Chicken 73.7 parts Chicken viscera 20 Vitamin / mineral mix 5 CPP 0.15 Dextran sulfate 0.15 After mixing the composition comprising the above ingredients with a mixer, 1.
Steaming treatment was performed in 5% saline at 100 ° C. for 10 minutes. After cooling, the meat was packed in a can to obtain a can type dog food. No. 4 can is used for canning, the weight of meat is 300 grams, water is 100 grams, and the temperature is 120 ° C for 70 minutes.
Retort sterilization was performed.

Example 5 Chicken 73.7 parts Chicken viscera 20 Vitamin / mineral mix 5 Calcium carbonate 1 CPP 0.15 Dextran sulfate 0.15 After mixing the composition comprising the above components with a mixer, 1.
Steaming treatment was performed in 5% saline at 100 ° C. for 10 minutes. After cooling, the meat was packed in a can to obtain a can type dog food. No. 4 can is used for canning, the weight of meat is 300 grams, water is 100 grams, and the temperature is 120 ° C for 70 minutes.
Retort sterilization was performed.

Example 6 Steamed Hematomeat 93.7 parts Vitamin / Mineral Mix 5 Calcium Carbonate 1 CPP 0.15 Mucin 0.15 A mixture of the above-mentioned composition mainly composed of the steamed bonito and tuna hematomeat Was made into flakes to obtain a can type cat food. Tuna No. 2 can was used for canning, the meat weight was 160 g, and water injection was 20 g, and retort sterilization was performed at 110 ° C. for 80 minutes.

Claims (2)

[Claims] 1. A pet food containing casein phosphopeptide (CPP), which comprises carrageenan, pectin,
1 selected from the group consisting of dextran sulfate and mucin
Pet food containing one or more species. 2. The pet food according to claim 1, fortified with calcium and / or iron.