JPH05123188A - Transfer and detection of collected cell specimen and transfer and detection vessel therefor - Google Patents

Abstract

PURPOSE:To easily and correctly detect a carrier in patients without using internal examination by adsorbing the urine of a patient to activated carbon using a specific method, transporting the adsorbed material to an examination organization and reacting a culture liquid with bacteria in the urine adsorbed to the activated carbon. CONSTITUTION:The urine of a patient is collected in an examination vessel containing an activated carbon 4 to effect the adsorption of the urine to the activated carbon 4. After discharging the urine from the vessel, the opening of the vessel is closed and the vessel is transported to an examination organization. A culture liquid is introduced into the examination vessel in the examination organization to effect the reaction of the culture liquid with the bacteria in the urine adsorbed to the activated carbon 4. The examination vessel is produced from a thermoplastic resin by integrally forming a thin tube 3 connected to the top 2 of a body tube 1, putting a piece of activated carbon 4 larger than the inner diameter of the thin tube 3 and pressing the lower part of the body tube 1 under heating to integrate the front part 6 and the back part of the body bottom 5 to form a flat face and make the bottom part 8 and the right and left side faces 9, 10 to lie on a straight line.

Description

Detailed Description of the Invention

[0001]

[Industrial field of application] In preventing the onset of neonatal group B streptococcal (GBS) infection, whether or not a pregnant woman carries GBS in the birth canal becomes a problem. The present invention relates to a method for detecting the transport of a collected specimen for determining whether or not the patient is carrying a bacterium, and a transport detection container used in the method.

[0002]

2. Description of the Related Art In order to detect GBS inhabiting a pregnant woman's birth canal, it has been customary to perform vaginal culture. It is also known that urine sediment culture has a higher detection rate than such vaginal culture.

[0003]

According to the above-mentioned conventional vaginal culture detection, the positive rate varies depending on the difference in the rubbing site with the cotton swab, the difference in the rubbing pressure, and the like. Further, from the 26th to the 36th week of the gestational weeks to be cultivated, it is difficult to obtain a constant and appropriate result because there are many institutions / facilities that do not undergo a pelvic examination during the pregnancy screening.

Under the circumstances described above, the present inventor has studied and studied a simpler method for collecting a specimen. As described above, it is known that the positivity rate is high if the urine sediment is cultured using urine at the time of pregnant woman screening, and the present inventor also compared the birth canal culture with the swab and the urine sediment culture. As a result, it was already confirmed that urine sediment culture had a higher positive rate.

However, in order to perform screening, it is necessary to perform serotyping of the detected GBS,
Judgment must be made by a certain judge at a certain institution / facility. For that purpose, it becomes complicated to deliver the urine sediment from each institution to a certain inspection institution,
In order to collect the urine sediment for culture, the hands of those technicians and nurses are troublesome. Since many of the facilities outsource testing, fewer facilities use a centrifuge permanently, and patients also have to go to a laboratory for internal examination. It was

As described above, the only method for detecting bacteria in urine was to deliver urine as it is or to transport urine in a medium. If urine is transported as it is, there is a risk of leakage of urine during transportation, and during the transportation period, bacteria having a strong proliferation ability may spread and bacteria having a weak proliferation ability may be killed. In view of the above-mentioned problems of the prior art, the present invention considers that a carrier in a patient should be easily and properly detected by transporting a sample without performing an internal examination.

[0007]

The present invention is intended to achieve the object by transporting a sample obtained by adsorbing bacteria in urine to activated carbon to a laboratory and reacting the sample with a culture solution. It is a thing.

Next, a specific configuration which is the gist of the present invention will be described using the reference numerals of the drawings corresponding to the embodiments. That is, the present invention collects the urine of a patient in a detection container containing activated carbon, adsorbs the urine on the activated carbon, discharges the urine in the detection container, and seals the opening to transport it to a laboratory. And, the transport detection of the collected specimen characterized by opening the closed part of the detection container, introducing the culture solution into the detection container, and reacting with the bacteria in the urine adsorbed on the activated carbon Is the way. And what to use for that method,
Explaining with reference to the reference numerals of the drawings corresponding to the embodiments, a thin tube 3 continuous upward from the upper end 2 of the body tube 1 is integrally molded with a thermoplastic rigid resin material, and the inner diameter d of the thin tube 3 is larger than the inner diameter d of the thin tube 3 in the body tube 1. By inserting a large amount of activated carbon 4 and thermocompression-bonding the bottom 5 of the body, the front part 6 and the back part 7 of the bottom 5 are made flat, and at the same time, the bottom part 8 and the left side part 9 and the right side part 1 are formed.
Another object is to provide a detection container for transporting a collected specimen, which is characterized in that 0 is formed in a straight line. In addition, it is convenient to make the cross section of the body tube 1 substantially elliptical.

[0009]

In the detection container configured as described above, the lower part of the detection container is naturally squeezed and continues to the bottom of the body.
The three-dimensional shape of the envelope can be further thinned by forming a similar shape along the bulge of the entire envelope from the bottom of the envelope at the time of transportation and forming the cross-sectional contour of the body tube in an oblong shape.

Since the body tube is made of a thermoplastic synthetic resin material and is flexible, if the body tube is pressed with a fingertip and the opening of the thin tube is put into the stored urine and the pressing is stopped, the urine becomes a thin tube. It is introduced into the body tube from and is adsorbed by the activated carbon scattered in the body tube. Further, the urine remaining in the body tube is discharged by pressing the body tube with the opening of the thin tube facing downward. At this time, the activated carbon is larger than the inner diameter of the thin tube,
It is not excreted with urine. Therefore, the activated carbon is scattered in the body tube while absorbing the urine. Further, as shown in FIG. 1, the opening of the thin tube can be closed by bending the thin tube and fitting the ring 11. This ring is flexible and convenient if it is made of a thermoplastic synthetic resin material like the main body, but is not limited to the ring, and may be appropriately bound with a string or a rubber band.

In the detection container thus sent to the inspection facility in the envelope, after cutting an appropriate part of the thin tube closed by the ring, the culture solution is put into the body tube by the same action as when collecting urine. If introduced into, it will react with urine adsorbed on activated carbon. Therefore, this detection container has a dropper function as described above, protects activated carbon, collects and discharges urine, and further introduces and discharges a culture solution,
It has an action / function such as performing a reaction.

[0012]

[Examples] With regard to pregnant women who had been examined at each laboratory or facility, urine collected for urine examination at the time of examination was used as a sample for basic experiments. After adding a certain amount of bacteria to this urine, the number of bacteria was calculated, and the urine was discarded after being adsorbed on activated carbon and left overnight. The activated carbon remaining in this way is
The samples were stored at 5 ° C, 37 ° C and 42 ° C for 5 days, SEB medium (Japanese water) was added thereto, and about 10 ml of urine was subjected to the following three kinds of operations. 1 ml of urine on selective blood agar plate containing antibiotics
The amount was poured, and 1 minute later, the urine was discarded and the cells were cultured (direct culture). Approximately 7 ml of urine was spun down at 3000 rpm for 10 minutes, and then 2 ml of SEB medium was added and stirred and then cultured (sediment culture). Four activated carbon particles were added to 2 ml of urine and allowed to stand overnight at room temperature, and then the urine was discarded and the SEB medium was added to the culture (activated carbon culture). As a result, in the basic experiment, even if the cells were stored at various temperatures for 5 days, a bacterial load of 10 ¹ (10 to the power of 1) was detectable. Of urine collected from 116 pregnant women, 58 were positive by any one of the above three culture methods.
It was an example. Among them, 18 were positive in direct culture.
Example (15.5%), 53 positive cases in sediment culture (45.
7%), and positive in activated carbon culture was 27 cases (23.3%). The results were 17 positive for all three species, 22 positive for sediment culture only, 4 positive for activated carbon only, and 5 positive for activated carbon and sediment culture.

As shown in the above results, the detection rate was the worst in the case of direct culture, followed by activated carbon culture and sediment culture. However, it is considered that the positive rate was extremely high in the sediment culture, and the positive rate was high because the cells were collected by the centrifugation operation even with a small amount of bacteria. When the serotypes of the detected bacteria are separated, the same serotype is detected from the same person regardless of which culture method is used, but a certain serotype is particularly often detected from the same laboratory. However, it is similar to the serotype distributions reported so far,
It was found that culture from general urine collection can also be adequately applied.

[0014]

According to the present invention, urine, which is a sample, is adsorbed by activated carbon and further transported while being sealed in a fixed container, so that leakage and evaporation of urine during transportation can be sufficiently prevented, and Urine remains extremely stable even when the temperature changes for several days. Therefore, even if the bacteria are not propagated or killed even during transportation, a constant positive rate can always be obtained as a result when attached to a sample. Moreover, since it is not based on an internal examination, it is possible to save a troublesome procedure for a patient and a person involved in a laboratory or facility. The shipping container according to the present invention, which is integrally molded of thermoplastic synthetic resin, has excellent productivity and is so-called “disposable”.
It is adaptable to, and is hygienic, and is not easily damaged during transportation and is highly safe. Further, urine collection and culture solution introduction can be performed in the same form. Further, the material and the shape are combined to make it lightweight and not bulky, and it can be easily sealed by bending and fixing the thin tube. Moreover,
Since it fits very well in the envelope, there is no movement or rattling inside the envelope and it is suitable for transportation.

[Brief description of drawings]

FIG. 1 is a perspective view showing an embodiment of the present invention.

FIG. 2 is a front view showing an embodiment of the present invention.

FIG. 3 is a vertical sectional view showing an embodiment of the present invention.

FIG. 4 is a plan view showing an embodiment of the present invention.

FIG. 5 is a bottom view showing an embodiment of the present invention.

FIG. 6 is a cross-sectional view showing an embodiment of the present invention.

[Explanation of symbols]

 1 Body tube 2 Upper end 3 Thin tube 4 Activated carbon 5 Body bottom 6 Front part 7 Back part 8 Bottom part 9 Left side part 10 Right side part 11 Ring d Inner diameter

Claims (3)

[Claims] 1. The urine of a patient is collected in a detection container containing activated carbon, the urine is adsorbed on the activated carbon, the urine in the detection container is discharged, and the opening is sealed and transported to a laboratory. And, the transport detection of the collected specimen characterized by opening the closed part of the detection container, introducing the culture solution into the detection container, and reacting with the bacteria in the urine adsorbed on the activated carbon Method. 2. A thin tube (3) continuous upward from the upper end (2) of the body tube (1) is integrally molded with a thermoplastic synthetic resin material,
By inserting activated carbon (4) larger than the inner diameter (d) of the thin tube (3) into the body tube (1) and thermocompressing the body bottom (5), the front part of the body bottom (5). (6) and the back surface part (7) are integrally flattened, and at the same time, the bottom surface part (8) and the left side surface part (9) and the right side surface part (10) are formed in a straight line. container. 3. The container for transporting a collected specimen according to claim 2, wherein the profile of the cross section of the body tube (1) is substantially elliptical.