JPH05123166A - Pr0pagati0n of cultured tea-cell colony - Google Patents

Pr0pagati0n of cultured tea-cell colony

Info

Publication number
JPH05123166A
JPH05123166A JP3314082A JP31408291A JPH05123166A JP H05123166 A JPH05123166 A JP H05123166A JP 3314082 A JP3314082 A JP 3314082A JP 31408291 A JP31408291 A JP 31408291A JP H05123166 A JPH05123166 A JP H05123166A
Authority
JP
Japan
Prior art keywords
tea
cultured cell
ethylamine
callus
cultured
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP3314082A
Other languages
Japanese (ja)
Inventor
Takanori Matsuura
孝宣 松浦
Takami Tsunoda
隆巳 角田
Naokazu Takeuchi
直和 竹内
Tatsuyuki Kinoshita
達之 木下
Kyosuke Sasaki
恭助 佐々木
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
FOOD DESIGN GIJUTSU KENKYU KUM
FOOD DESIGN GIJUTSU KENKYU KUMIAI
Original Assignee
FOOD DESIGN GIJUTSU KENKYU KUM
FOOD DESIGN GIJUTSU KENKYU KUMIAI
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by FOOD DESIGN GIJUTSU KENKYU KUM, FOOD DESIGN GIJUTSU KENKYU KUMIAI filed Critical FOOD DESIGN GIJUTSU KENKYU KUM
Priority to JP3314082A priority Critical patent/JPH05123166A/en
Publication of JPH05123166A publication Critical patent/JPH05123166A/en
Pending legal-status Critical Current

Links

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE:To shorten the culturing period and to promote proliferation of cultured cell colonies useful as a raw material for producing useful components by improving the proliferation ratio of callus and cells in tissue culture of tea. CONSTITUTION:Cultured cell colonies of tea are cultured in an ethylamine- containing culture medium. In this culture, the concentration of ethylamine in the culture medium is adjusted to a prescribed value within a range of 2-95mM. Thereby, proliferation of the cultured cell colonies can be promoted while increasing the amount of accumulated theanine in the cultured cell colonies.

Description

【発明の詳細な説明】Detailed Description of the Invention

【0001】[0001]

【産業上の利用分野】本発明は、茶の培養細胞群、即ち
カルス及び細胞の増殖促進を図る茶の培養細胞群の増殖
法に関する。
BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for growing a cultured cell group of tea, that is, a cultured cell group of tea for promoting the growth of callus and cells.

【0002】[0002]

【従来の技術】茶の組織培養において、茶のカルスは非
常に硬く増殖が遅いことが特徴であり、大量増殖及び有
用物質の生産等の研究において、この点を改善すること
が必要とされている。一方、茶のうま味成分であり、カ
フェインの有する興奮作用を抑制するテアニンは、茶葉
中には1.5%程度含有されているが、カルス中にはほ
とんど含有されていない。カルス及び細胞の増殖促進を
図るために、培地中に含まれる無機塩、ビタミン類、及
び植物生長調節物質等の検討が行われてきたが、未だ茶
の培養細胞群の増殖率は低いのが現状である。
2. Description of the Related Art In the tissue culture of tea, tea callus is characterized by being extremely hard and proliferating slowly, and it is necessary to improve this point in studies such as mass growth and production of useful substances. There is. On the other hand, theanine, which is an umami component of tea and suppresses the excitatory effect of caffeine, is contained in tea leaves in an amount of about 1.5%, but is hardly contained in callus. In order to promote the growth of callus and cells, inorganic salts, vitamins, and plant growth regulators contained in the medium have been studied, but the growth rate of the cultured cell group of tea is still low. The current situation.

【0003】[0003]

【発明が解決しようとする課題】この様なカルス及び細
胞の増殖率の低さは、茶の組織培養による培養あるいは
有用成分の生産において大きな問題となっている。それ
故、新たな手法による培養細胞群の増殖法の開発が必要
とされているのである。本発明は、かかる課題に鑑みて
なされたものであり、茶の組織培養におけるカルス及び
細胞の増殖率を高め、培養期間の短縮及び有用成分の生
産のための材料となる培養細胞群の増殖促進を図ること
を目的とする。
Such a low growth rate of callus and cells has become a serious problem in the culture of tea tissue culture or the production of useful ingredients. Therefore, it is necessary to develop a new method for expanding cultured cell populations. The present invention has been made in view of the above problems, and enhances the growth rate of callus and cells in the tissue culture of tea, shortens the culture period and promotes the growth of a cultured cell group as a material for the production of useful ingredients. The purpose is to

【0004】[0004]

【課題を解決するための手段】本発明者らは上記課題を
解決するために種々検討、研究を重ねた結果、茶のカル
ス及び細胞をエチルアミンを含有する培地で培養するこ
とにより、培養細胞群中のテアニン蓄積量を増加させつ
つ培養細胞群の増殖率を高めることができることを見い
だし、本発明に想到したものである。
Means for Solving the Problems As a result of various investigations and studies for solving the above-mentioned problems, the present inventors have found that callus and cells of tea are cultured in a medium containing ethylamine to obtain a cultured cell group. The inventors have found that it is possible to increase the growth rate of the cultured cell group while increasing the amount of theanine accumulated therein, and have conceived the present invention.

【0005】即ち、本発明は、茶のカルス及び細胞培養
において、エチルアミンを含有する培地の使用及びその
濃度調整により、培養細胞群中のテアニン蓄積量を増加
させつつ培養細胞群の増殖促進を図ることを特徴とする
ものである。
That is, the present invention aims to promote the growth of cultured cell groups while increasing the amount of theanine accumulated in the cultured cell groups by using a medium containing ethylamine and adjusting the concentration thereof in the culture of tea callus and cells. It is characterized by that.

【0006】培地中のエチルアミン濃度を培養開始前に
2〜95mMの範囲の所定値に調整し、例えばカルスを
25℃・暗黒下において3週間液体振とう培養(100
rpm)することにより、エチルアミンを含有しない培
地での培養に比して、カルス内にテアニンの蓄積量が増
加しつつカルスの増殖率が高まることが明かとなった。
ここで、培地中のエチルアミン濃度は10〜75mMの
範囲の所定値に調整するのが特に好ましく、培養細胞群
の増殖促進効果は顕著である。
The concentration of ethylamine in the medium is adjusted to a predetermined value in the range of 2 to 95 mM before the start of the culture, and, for example, callus is cultured at 25 ° C. in the dark for 3 weeks in liquid shaking culture (100
It was revealed that the growth rate of callus was increased by increasing the amount of theanine accumulated in the callus as compared with the culture in the medium containing no ethylamine.
Here, it is particularly preferable to adjust the concentration of ethylamine in the medium to a predetermined value within the range of 10 to 75 mM, and the effect of promoting the growth of the cultured cell group is remarkable.

【0007】[0007]

【発明の効果】本発明の茶の培養細胞群の増殖法によれ
ば、茶の組織培養におけるカルス及び細胞の増殖率を高
め、培養期間の短縮及び有用成分の生産のための材料と
なる培養細胞群の増殖を促進することができる。又、同
時に、茶の有用成分であるテアニンの蓄積量を増加させ
て組織培養における茶由来のテアニンの生産を増大する
こともできる。
EFFECTS OF THE INVENTION According to the method for growing a cultured cell group of tea of the present invention, a culture that serves as a material for increasing the growth rate of callus and cells in tissue culture of tea, shortening the culture period and producing useful ingredients. It is possible to promote the growth of cell groups. At the same time, it is also possible to increase the amount of theanine accumulated, which is a useful component of tea, to increase the production of tea-derived theanine in tissue culture.

【0008】[0008]

【実施例】次に本発明を一実施例により説明する。EXAMPLES The present invention will be described below with reference to examples.

【0009】表1に示した成分からなる培地を基本培地
とし、エチルアミン濃度を表2の濃度に調整した培地を
作成し、茶カルスの増殖に及ぼす影響をみた。基本培地
に寒天(0.8%)を添加した固形培地上(25℃・暗
黒下)で培養してきたカルス(1g)をエチルアミン濃
度が調整された液体培地(30ml)を用いて、25℃
・暗黒下で3週間振とう培養(100rpm)を行っ
た。1区当たり3サンプルとした。エチルアミン及びテ
アニンは高速液体クロマトグラフィーを用いて測定し
た。
Using the medium consisting of the components shown in Table 1 as the basic medium, a medium was prepared in which the concentration of ethylamine was adjusted to the concentration shown in Table 2, and the effect on the growth of tea callus was observed. Callus (1 g), which had been cultivated on a solid medium (25 ° C. in the dark) to which agar (0.8%) was added to a basal medium, was used at 25 ° C. using a liquid medium (30 ml) in which the concentration of ethylamine was adjusted.
-Shaking culture (100 rpm) was performed in the dark for 3 weeks. There were 3 samples per ward. Ethylamine and theanine were measured using high performance liquid chromatography.

【0010】[0010]

【表1】 [Table 1]

【0011】その結果、表2に示した通り、培地中のエ
チルアミンの初期濃度を2〜95mMの範囲内の所定値
となるように調整した区での増殖率は30%以上とな
り、エチルアミン無添加区の26.3%に比して実験誤
差以上の明確な培養カルスの増殖促進効果があることが
認められた。特に、培地中のエチルアミンの初期濃度を
10〜75mMの範囲内の所定値となるように調整した
区では、エチルアミン無添加区に比べて顕著な増殖率の
向上が認められた。又同時に、エチルアミン無添加区に
比してテアニンの蓄積量も大量に増加していることが認
められた。
As a result, as shown in Table 2, the growth rate in the group in which the initial concentration of ethylamine in the medium was adjusted to a predetermined value within the range of 2 to 95 mM was 30% or more, and no ethylamine was added. It was confirmed that there was a clear growth-promoting effect on the cultured callus with an experimental error or more as compared with 26.3% of the plot. Particularly, in the group in which the initial concentration of ethylamine in the medium was adjusted to a predetermined value within the range of 10 to 75 mM, a remarkable improvement in the growth rate was recognized as compared with the group in which no ethylamine was added. At the same time, it was also confirmed that the amount of theanine accumulated was increased in a large amount as compared with the group without addition of ethylamine.

【0012】[0012]

【表2】 [Table 2]

───────────────────────────────────────────────────── フロントページの続き (72)発明者 竹内 直和 愛知県名古屋市中村区岩塚町字高道一番地 三菱重工業株式会社名古屋研究所内 (72)発明者 木下 達之 愛知県名古屋市中村区岩塚町字高道一番地 三菱重工業株式会社名古屋研究所内 (72)発明者 佐々木 恭助 愛知県西春日井郡西枇杷島町旭町3−1 三菱重工業株式会社エアコン製作所内 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Naokazu Takeuchi Highway No. 1 in Iwazuka Town, Nakamura-ku, Nagoya, Aichi Prefecture Mitsubishi Heavy Industries, Ltd. Nagoya Research Laboratory (72) Inventor Tatsuyuki Kinoshita Iwatsuka, Nakamura-ku, Nagoya, Aichi Prefecture The first place of the highway in the town Mitsubishi Heavy Industries Ltd. Nagoya Research Institute (72) Inventor Kyosuke Sasaki 3-1 Asahicho, Nishibirashima-cho, Nishikasugai-gun, Aichi Mitsubishi Heavy Industries, Ltd.

Claims (2)

【特許請求の範囲】[Claims] 【請求項1】 茶の培養細胞群をエチルアミンを含有す
る培地で培養して培養細胞群の増殖率を高めることを特
徴とする茶の培養細胞群の増殖法。
1. A method for growing a cultured cell group of tea, which comprises culturing the cultured cell group of tea in a medium containing ethylamine to increase the growth rate of the cultured cell group.
【請求項2】 培地中のエチルアミン濃度を2〜95m
Mの範囲内の所定値に調整することを特徴とする請求項
1に記載の茶の培養細胞群の増殖法。
2. The concentration of ethylamine in the medium is 2 to 95 m.
The method for growing a cultured cell group of tea according to claim 1, wherein the method is adjusted to a predetermined value within the range of M.
JP3314082A 1991-10-31 1991-10-31 Pr0pagati0n of cultured tea-cell colony Pending JPH05123166A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP3314082A JPH05123166A (en) 1991-10-31 1991-10-31 Pr0pagati0n of cultured tea-cell colony

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP3314082A JPH05123166A (en) 1991-10-31 1991-10-31 Pr0pagati0n of cultured tea-cell colony

Publications (1)

Publication Number Publication Date
JPH05123166A true JPH05123166A (en) 1993-05-21

Family

ID=18049018

Family Applications (1)

Application Number Title Priority Date Filing Date
JP3314082A Pending JPH05123166A (en) 1991-10-31 1991-10-31 Pr0pagati0n of cultured tea-cell colony

Country Status (1)

Country Link
JP (1) JPH05123166A (en)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7094787B2 (en) 2000-07-21 2006-08-22 Taiyo Kagaku Co., Ltd. Compositions for regulating desire for smoking
WO2006118090A1 (en) 2005-04-28 2006-11-09 Taiyokagaku Co., Ltd. Water-containing food
WO2007098931A1 (en) * 2006-03-03 2007-09-07 Unilever Plc Process for preparing a tea product
US7273621B2 (en) 2000-04-05 2007-09-25 Taiyo Kagaku Co., Ltd. Compositions for promoting sleep
WO2008036809A2 (en) 2006-09-21 2008-03-27 Bukowski, Jack, F. Methods and materials for reducing risk of cold and/or flu
WO2008036807A2 (en) 2006-09-21 2008-03-27 Bukowski Jack F Tea-derived compostiions and methods of using same for cardiovascular health
WO2014117176A1 (en) 2013-01-28 2014-07-31 Lopez Hector L Methods of improving tolerability, pharmacodynamics, and efficacy of b-alanine and use therefor

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7273621B2 (en) 2000-04-05 2007-09-25 Taiyo Kagaku Co., Ltd. Compositions for promoting sleep
US8293269B2 (en) 2000-04-05 2012-10-23 Taiyo Kagaku Co., Ltd. Compositions for promoting sleep
US7094787B2 (en) 2000-07-21 2006-08-22 Taiyo Kagaku Co., Ltd. Compositions for regulating desire for smoking
WO2006118090A1 (en) 2005-04-28 2006-11-09 Taiyokagaku Co., Ltd. Water-containing food
WO2007098931A1 (en) * 2006-03-03 2007-09-07 Unilever Plc Process for preparing a tea product
WO2008036809A2 (en) 2006-09-21 2008-03-27 Bukowski, Jack, F. Methods and materials for reducing risk of cold and/or flu
WO2008036807A2 (en) 2006-09-21 2008-03-27 Bukowski Jack F Tea-derived compostiions and methods of using same for cardiovascular health
WO2014117176A1 (en) 2013-01-28 2014-07-31 Lopez Hector L Methods of improving tolerability, pharmacodynamics, and efficacy of b-alanine and use therefor

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