JPH05123166A - Pr0pagati0n of cultured tea-cell colony - Google Patents

Abstract

PURPOSE:To shorten the culturing period and to promote proliferation of cultured cell colonies useful as a raw material for producing useful components by improving the proliferation ratio of callus and cells in tissue culture of tea. CONSTITUTION:Cultured cell colonies of tea are cultured in an ethylamine- containing culture medium. In this culture, the concentration of ethylamine in the culture medium is adjusted to a prescribed value within a range of 2-95mM. Thereby, proliferation of the cultured cell colonies can be promoted while increasing the amount of accumulated theanine in the cultured cell colonies.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for growing a cultured cell group of tea, that is, a cultured cell group of tea for promoting the growth of callus and cells.

[0002]

2. Description of the Related Art In the tissue culture of tea, tea callus is characterized by being extremely hard and proliferating slowly, and it is necessary to improve this point in studies such as mass growth and production of useful substances. There is. On the other hand, theanine, which is an umami component of tea and suppresses the excitatory effect of caffeine, is contained in tea leaves in an amount of about 1.5%, but is hardly contained in callus. In order to promote the growth of callus and cells, inorganic salts, vitamins, and plant growth regulators contained in the medium have been studied, but the growth rate of the cultured cell group of tea is still low. The current situation.

[0003]

Such a low growth rate of callus and cells has become a serious problem in the culture of tea tissue culture or the production of useful ingredients. Therefore, it is necessary to develop a new method for expanding cultured cell populations. The present invention has been made in view of the above problems, and enhances the growth rate of callus and cells in the tissue culture of tea, shortens the culture period and promotes the growth of a cultured cell group as a material for the production of useful ingredients. The purpose is to

[0004]

Means for Solving the Problems As a result of various investigations and studies for solving the above-mentioned problems, the present inventors have found that callus and cells of tea are cultured in a medium containing ethylamine to obtain a cultured cell group. The inventors have found that it is possible to increase the growth rate of the cultured cell group while increasing the amount of theanine accumulated therein, and have conceived the present invention.

That is, the present invention aims to promote the growth of cultured cell groups while increasing the amount of theanine accumulated in the cultured cell groups by using a medium containing ethylamine and adjusting the concentration thereof in the culture of tea callus and cells. It is characterized by that.

The concentration of ethylamine in the medium is adjusted to a predetermined value in the range of 2 to 95 mM before the start of the culture, and, for example, callus is cultured at 25 ° C. in the dark for 3 weeks in liquid shaking culture (100
It was revealed that the growth rate of callus was increased by increasing the amount of theanine accumulated in the callus as compared with the culture in the medium containing no ethylamine.
Here, it is particularly preferable to adjust the concentration of ethylamine in the medium to a predetermined value within the range of 10 to 75 mM, and the effect of promoting the growth of the cultured cell group is remarkable.

[0007]

EFFECTS OF THE INVENTION According to the method for growing a cultured cell group of tea of the present invention, a culture that serves as a material for increasing the growth rate of callus and cells in tissue culture of tea, shortening the culture period and producing useful ingredients. It is possible to promote the growth of cell groups. At the same time, it is also possible to increase the amount of theanine accumulated, which is a useful component of tea, to increase the production of tea-derived theanine in tissue culture.

[0008]

EXAMPLES The present invention will be described below with reference to examples.

Using the medium consisting of the components shown in Table 1 as the basic medium, a medium was prepared in which the concentration of ethylamine was adjusted to the concentration shown in Table 2, and the effect on the growth of tea callus was observed. Callus (1 g), which had been cultivated on a solid medium (25 ° C. in the dark) to which agar (0.8%) was added to a basal medium, was used at 25 ° C. using a liquid medium (30 ml) in which the concentration of ethylamine was adjusted.
-Shaking culture (100 rpm) was performed in the dark for 3 weeks. There were 3 samples per ward. Ethylamine and theanine were measured using high performance liquid chromatography.

[0010]

[Table 1]

As a result, as shown in Table 2, the growth rate in the group in which the initial concentration of ethylamine in the medium was adjusted to a predetermined value within the range of 2 to 95 mM was 30% or more, and no ethylamine was added. It was confirmed that there was a clear growth-promoting effect on the cultured callus with an experimental error or more as compared with 26.3% of the plot. Particularly, in the group in which the initial concentration of ethylamine in the medium was adjusted to a predetermined value within the range of 10 to 75 mM, a remarkable improvement in the growth rate was recognized as compared with the group in which no ethylamine was added. At the same time, it was also confirmed that the amount of theanine accumulated was increased in a large amount as compared with the group without addition of ethylamine.

[0012]

[Table 2]

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Naokazu Takeuchi Highway No. 1 in Iwazuka Town, Nakamura-ku, Nagoya, Aichi Prefecture Mitsubishi Heavy Industries, Ltd. Nagoya Research Laboratory (72) Inventor Tatsuyuki Kinoshita Iwatsuka, Nakamura-ku, Nagoya, Aichi Prefecture The first place of the highway in the town Mitsubishi Heavy Industries Ltd. Nagoya Research Institute (72) Inventor Kyosuke Sasaki 3-1 Asahicho, Nishibirashima-cho, Nishikasugai-gun, Aichi Mitsubishi Heavy Industries, Ltd.

Claims (2)

[Claims] 1. A method for growing a cultured cell group of tea, which comprises culturing the cultured cell group of tea in a medium containing ethylamine to increase the growth rate of the cultured cell group. 2. The concentration of ethylamine in the medium is 2 to 95 m.
The method for growing a cultured cell group of tea according to claim 1, wherein the method is adjusted to a predetermined value within the range of M.