SU1761058A1 - Method for preparation of cotton fiber - Google Patents

Abstract

Usage: biotechnologists, cultivation of tissues and cells, cotton growing. SUMMARY OF THE INVENTION: Seed cotton buds are implanted onto a modified Bisley-Ting solid nutrient medium, in which ferulic and gibberellic acid are additionally introduced at a concentration of 0.1 mg / l each and cultivation is carried out for 36 days. 1 з п. Ф-лы, 1 tabl

Description

This invention relates to biology, in particular cotton biotechnology.

Earlier in the experiments, the effect of giberrellic, indolylacetic acid and kinetin on fiber growth in the seed culture of cotton buds was shown. The use of these phytohormones allowed the author to find the best way to grow seed in culture. It follows from Bisley’s description that macro- and micro-salts, vitamins and sugars are included in the nutrient medium.

According to Bisley, fiber growth on artificial medium with phytohormone supplements is carried out up to 14 days of age. Such an age of fiber is considered to be quite sufficient to study the effect of growth substances on fiber growth.

The disadvantage of the Bisley method is that its (method) cannot be used when it is necessary to obtain fibers more than 14 days old. (To study the mechanisms of cellulose biosynthesis,

accumulate as much of the total fiber mass as possible).

The aim of the invention is to increase the fiber yield and lengthen the growth period. This method is characterized in that ferulic and gibberellic acids are additionally introduced into the nutrient medium at a concentration of 0.1 mg / l and the cultivation is carried out on a solid nutrient medium, which is prepared as follows:

KH2P04

CMOS

CaCIa H20

Callback

MnS04

ZnS04 7H20

Kl

Na2Mo04

Myoinositis

D-glucose

MgS04 H20

Edta

5.44 g

101.11

8.82

6.183

16.9С

8,627

0.83

0,242

0.18

18,016

9.86

2.23

(L

with

 about

about ate

00

FeS04 H20 CuS04 5H20 CoCl2 6H20 Pyrodoxin chloride Thiamine chloride Nicotinic acid Fructose Agar

0.167

0.025

0.024

0.0411

0.0411

0.0568

3.6032

7

Furulova and gibberrellic acid are also introduced at a concentration of 0.1 mg / l.

All samples were taken on 1 liter of distilled water. pH of the medium - 5.4 - 5.5. All further operations were carried out in relation to our conditions, as well as preparation of the medium.

The resulting medium was poured into 50 ml Erlenmeyer cones and autoclaved for 15 min at 0.8 - 1 atm. Before autoclaving, growth regulators were introduced into the nutrient mixture in various concentrations and combinations, which will be described below. Under aseptic conditions, seed buds were planted on the nutrient medium and placed in a biological thermostat at 32 ° C. The fiber was grown for 14 days and then transplanted into other cones with the same nutrient mixture, but with the addition of DNA + RNA + ATP. This method ensures the growth and accumulation of the total fiber mass, in contrast to the Beasley method, for 36 days. In addition, the introduction into the nutrient mixture of ferulic acid in a mixture with gibberellic acid provides for the accumulation of the total mass of fiber more than 4 times compared with the control. The data are given in the table.

The total mass of the fiber was determined by the toluidine method. For this, they took 10 seeds of buds, on the surface of which a fiber was formed, were placed in a conical flask per 100 ml of mixture per 1 seed of a kidney. Then the flask was placed on a rocking chair and shaken for 2 minutes. After shaking the seeds, the kidneys with fiber were washed in a funnel with a filter, first with the same nutrient mixture and then with water. After washing the seeds, the kidneys were transferred to cones with a solution of toluidine blue in a buffer of pH 4.5, consisting of 10, 14 ml of 0, OR citric acid + 9.86 ml of 0.016 M Na2HP04 2HaO. A solution of toluidine was poured at the rate of 4 ml for each sample for each seed kidney with fiber. Staining was carried out for 15–20 s, after which the precipitate was filtered and the precipitate was washed with distilled water. Then this kidney seed with fiber was placed in a Cornwall solution, poured into Erlenmeyer flasks at the rate of 5 ml of solution per 1 seed of a kidney, i.e., 50 ml of Kornua solution should be used for 10 seed buds with fiber. One hour after placing the seeds of the kidneys into the Cornwall solution, the optical density of the colored solution on SF-4 was measured at a wavelength of 640 mm. The calculation of the total mass of the fiber was established by the standard curve, as indicated in the work of Beasley.

This method of fiber accumulation is offered for the first time and has an enormous social effect, as it will release large reserves of raw cotton for the textile industry.

Thus, at present, for the production of cellulose, predominantly high quality raw cotton is used, which could be successfully used for the production of cotton fabrics.

The application of the above method opens up a new technology for the production and accumulation of the total mass of fiber on this kidney of the lower rushes of cotton, which in field conditions almost always fall off.

The tests were carried out for 4 years at the Institute of Experimental Biology of Plants of the Academy of Sciences of the Uzbek SSR.

Claims (2)

1. A method of obtaining cotton fiber, including explanting kidney seeds onto Bisley's modified nutrient medium and subsequent tissue cultivation, characterized in that, in order to increase the fiber yield and lengthen its growth period, ferulic and gibberellic acid is added to the nutrient medium 0.1 mg / l each and the culture was performed on solid nutrient medium. 2. A pop method, 1, characterized in that the agar is additionally added to the nutrient medium as a compactor in an amount of 7 g / l. The accumulation of the total mass of cotton fiber varieties Tashkent-1 (age 36 days)