JPH0495914A - Translucent slide glass - Google Patents
Translucent slide glassInfo
- Publication number
- JPH0495914A JPH0495914A JP20821090A JP20821090A JPH0495914A JP H0495914 A JPH0495914 A JP H0495914A JP 20821090 A JP20821090 A JP 20821090A JP 20821090 A JP20821090 A JP 20821090A JP H0495914 A JPH0495914 A JP H0495914A
- Authority
- JP
- Japan
- Prior art keywords
- slide glass
- porous filter
- translucent
- light transmittance
- acrylic resin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000011521 glass Substances 0.000 title claims abstract description 55
- 238000002834 transmittance Methods 0.000 claims abstract description 42
- 238000009792 diffusion process Methods 0.000 claims abstract description 37
- 239000012798 spherical particle Substances 0.000 claims abstract description 18
- 239000004925 Acrylic resin Substances 0.000 claims abstract description 16
- 229920000178 Acrylic resin Polymers 0.000 claims abstract description 16
- 239000002245 particle Substances 0.000 claims abstract description 15
- 229920002050 silicone resin Polymers 0.000 claims abstract description 14
- 239000000126 substance Substances 0.000 abstract description 5
- 238000000034 method Methods 0.000 description 24
- 239000003795 chemical substances by application Substances 0.000 description 18
- 239000011148 porous material Substances 0.000 description 15
- 238000001125 extrusion Methods 0.000 description 11
- 238000000465 moulding Methods 0.000 description 10
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 description 9
- 230000006835 compression Effects 0.000 description 8
- 238000007906 compression Methods 0.000 description 8
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 8
- 239000004926 polymethyl methacrylate Substances 0.000 description 8
- 229920005989 resin Polymers 0.000 description 7
- 239000011347 resin Substances 0.000 description 7
- 239000000758 substrate Substances 0.000 description 7
- 239000000463 material Substances 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 229920001296 polysiloxane Polymers 0.000 description 4
- 238000009826 distribution Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000007689 inspection Methods 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 230000000704 physical effect Effects 0.000 description 3
- 238000004062 sedimentation Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000006185 dispersion Substances 0.000 description 2
- 235000019441 ethanol Nutrition 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 229920000800 acrylic rubber Polymers 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- XTKDAFGWCDAMPY-UHFFFAOYSA-N azaperone Chemical compound C1=CC(F)=CC=C1C(=O)CCCN1CCN(C=2N=CC=CC=2)CC1 XTKDAFGWCDAMPY-UHFFFAOYSA-N 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000005388 borosilicate glass Substances 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000000748 compression moulding Methods 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 239000006059 cover glass Substances 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000012943 hotmelt Substances 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 229920006289 polycarbonate film Polymers 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 229920003002 synthetic resin Polymers 0.000 description 1
- 239000000057 synthetic resin Substances 0.000 description 1
- 229920006027 ternary co-polymer Polymers 0.000 description 1
- 238000001132 ultrasonic dispersion Methods 0.000 description 1
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野］
本発明は、例えば生体細胞、細菌、ウィルス等の生物学
並びに医学上の顕微鏡観察に用いる半透明スライドガラ
スに関する。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a translucent slide glass used for biological and medical microscopic observation of biological cells, bacteria, viruses, etc., for example.
一般に、細胞検査、細菌類の確認、薬効テスト等の生物
並びに医学研究用の顕微鏡観察は、例えば細胞診の場合
、被検体を重なり合わないようスライドガラス５に付け
（塗床）、被検体が脱落しやすいスライドガラス上での
被検体の固定化、染色及び洗浄を行った上で顕微鏡観察
に供するという、熟練度、手間及び時間のかかる処理を
経て行われている。In general, microscopic observations for biological and medical research such as cell examinations, confirmation of bacteria, and drug efficacy tests are carried out by attaching the specimen to a slide glass 5 (painted bed) so as not to overlap, for example, in the case of cytodiagnosis. This is done through a highly skilled, labor-intensive and time-consuming process in which the specimen is fixed on a glass slide, which easily falls off, stained, washed, and then subjected to microscopic observation.
上記処理の実状から、最近、多孔性フィルター３を用い
て培養液中の被検体を捕捉し、被検体に対してこの多孔
性フィルター３で固定化、染色及び洗浄処理を施して顕
微鏡観察に供する検査システムが注目されている。この
新しい検査システムによると、被検体の重なり合いを生
じることなく容易に捕捉出来ると共に、多孔性フィルタ
ー上の被検体が脱落しにくいので、固定化、染色及び洗
浄処理が容易で、これまでの処理のような熟練度を要し
ないばかりか、手間及び処理時間を大幅に軽減出来るも
のと期待されている。In view of the actual state of the above-mentioned processing, recently, a porous filter 3 is used to capture a specimen in a culture solution, and the specimen is fixed with this porous filter 3, stained and washed, and then subjected to microscopic observation. Inspection systems are attracting attention. According to this new testing system, it is possible to easily capture specimens without overlapping them, and since the specimens on the porous filter are difficult to fall off, fixation, staining, and washing are easier, making it easier to perform fixation, staining, and washing. This method not only does not require the same level of skill, but is also expected to significantly reduce labor and processing time.
ところで、被検体を捕捉した上記多孔性フィルターをそ
まま透明なスライドガラス上に置いて顕微鏡観察したの
では、多孔性フィルターの孔の輪郭が被検体２と重なっ
て見えてしまい、観察の妨げとなる。By the way, if the above-mentioned porous filter that has captured the specimen is placed on a transparent slide glass and observed under a microscope, the outline of the pores of the porous filter will overlap with the specimen 2, which will interfere with the observation. Become.
これを防止するために、多孔性フィルター上に捕捉した
被検体を透明なスライドガラス上に転写してから観察す
ることや、透明なスライドガラス上で多孔性フィルター
を溶剤で溶解除去してから観察することが一部で行われ
ている。しかし、これらは、被検体の脱落なく転写や溶
液の除去を行うのに熟練と多大な手間及び時間を要し、
実用的なものとはなっていない。To prevent this, it is possible to transfer the sample captured on a porous filter onto a transparent slide glass before observation, or to remove the porous filter by dissolving it with a solvent on a transparent slide glass before observing it. This is being done in some areas. However, these methods require skill and a great deal of effort and time to transfer and remove the solution without causing the specimen to fall off.
It is not practical.
そこで、このような熟練性、手間及び時間を要せずして
、多孔性フィルターの孔による観察障害を防止するため
、被検体を捕捉した多孔性フィルターを半透明スライド
ガラス上に載せてそのまま観察することが提案されてい
る（特開昭６４−６９１４号公報）。Therefore, in order to prevent observation problems caused by the pores of the porous filter without requiring such skill, effort, and time, the porous filter that has captured the sample can be placed on a translucent slide glass and observed as it is. It has been proposed to do so (Japanese Unexamined Patent Publication No. 64-6914).
上記観察の特徴は、−船釣透明なでスライドガラスでは
なく、光の散乱作用が得られる半透明スライドガラスを
用いている点にある。そして、このような半透明スライ
ドガラスを使用しているのは、顕微鏡の光源からの光を
散乱させることで工学的に多孔性フィルターの輪郭が見
えなくなるようにするためである。The above-mentioned observation is characterized by the fact that it uses a translucent slide glass that can scatter light, rather than a transparent slide glass. The purpose of using such a translucent glass slide is to scatter the light from the light source of the microscope, so that the outline of the porous filter cannot be seen from an engineering point of view.
［発明が解決しようとする課題］
しかし、上記半透明スライドガラスは従来のスライドガ
ラスに比べて高価であり数倍から数十倍のコストがかか
る。そして、この半透明スライドガラスは被検体ごとに
一枚づつ使用しなければならず、したがって不経済であ
る。[Problems to be Solved by the Invention] However, the above-mentioned translucent slide glass is more expensive than conventional slide glass, and costs several to several tens of times more. Moreover, one translucent glass slide must be used for each subject, which is therefore uneconomical.
また半透明スライドガラスの使用によって、観察時に多
孔性フィルターの孔の輪郭が見えなくなるようにするこ
とを開示している前記特開昭６４６９１４号公報は、そ
の基本原理は開示しているものの、具体的にどのような
半透明スライドガラスが有効であるかまでは開示してい
ない。Furthermore, although the aforementioned Japanese Patent Application Laid-open No. 646914, which discloses the use of a translucent slide glass to make the outline of the pores of a porous filter invisible during observation, discloses the basic principle, It is not disclosed what kind of translucent slide glass is effectively used.
半透明スライドガラスといっても、単に従来から広く使
用されている透明なスライドガラスを半透明化すればよ
いというものではなく、多孔性フィルターの孔の輪郭が
見えなくするための光拡散作用と同時に、高倍率下でも
十分な視野の明るさが得られる透過光の確保という要求
を満足させる必要がある。Translucent slide glass does not simply mean that the conventionally widely used transparent slide glass has been made translucent; it has a light-diffusing effect that makes the outline of the pores of a porous filter invisible. At the same time, it is necessary to satisfy the requirement of ensuring transmitted light that provides sufficient brightness of the field of view even under high magnification.
前述したように、多孔性フィルターを用いた検査システ
ム、これまでの非効率的な作業を根本的に改善出来る可
能性を有しているにもかかわらず、不経済であり、また
具体的な半透明スライドガラスの提供がないため、真に
実用的な検査システムとはなっていないのが現状である
。As mentioned above, although inspection systems using porous filters have the potential to fundamentally improve the inefficient work that has been done up until now, they are uneconomical and do not require concrete semi-finishing. Currently, it is not a truly practical inspection system because a transparent slide glass is not provided.
そこで説明者等は、この半透明スライドガラスの材料に
ついて鋭意検討した結果、液晶カラーテレビ等のバック
ライト光源の拡散板に使用される（特開平１−１７２８
０１号公報）光硬化樹脂性光拡散板等が良好であること
を発見した。しかし、これら拡散板の製造コストは高く
、また物性も十分には満足のいくものではなかった。As a result of intensive study on the material of this translucent slide glass, presenters and others found that it is used as a diffuser plate for the backlight light source of LCD color televisions, etc. (Japanese Patent Laid-Open No. 1-1728
It has been discovered that a light-curing resin light diffusing plate (No. 01) is good. However, the manufacturing cost of these diffusion plates is high, and the physical properties are not fully satisfactory.
本発明は、このような現状に鑑みなされたもので上記ス
ライドガラスの材料に関するもので従来の拡散板に存在
する不経済性及び物性の不十分さを解決する材料を提供
することを目的とするものである。The present invention was made in view of the current situation, and relates to the material for the slide glass mentioned above, and an object of the present invention is to provide a material that solves the uneconomical problems and insufficient physical properties that exist in conventional diffuser plates. It is something.
〔課題を解決するための手段及び作用〕即ち、本発明は
、数平均粒子径０．３〜４μｍのシリコーン樹脂の球状
粒子を含有する、配向した拡散剤入りアクリル樹脂から
なり、全光線透過率が３０％以上、平行光線透過率が６
％以下、ヘイズ度が８５％以上である光拡散層を有する
半透明スライドガラスを提供することにある。[Means and effects for solving the problem] That is, the present invention consists of an oriented acrylic resin containing a diffusing agent, which contains spherical particles of silicone resin with a number average particle diameter of 0.3 to 4 μm, and has a total light transmittance of is 30% or more, parallel light transmittance is 6
% or less, and a haze degree of 85% or more.
本発明を図に基づいて更に詳細に説明する。The present invention will be explained in more detail based on the drawings.
本発明の半透明スライドガラス５は、前記した多孔性フ
ィルター３をその上に置いて、当該多孔性フィルター３
に捕捉されている被検体２を顕微−観察する際に、光源
からの光を散乱させて、多孔性フィルター３の孔の輪郭
が見えなくするものである。The translucent slide glass 5 of the present invention can be prepared by placing the above-mentioned porous filter 3 thereon.
When performing microscopic observation of the subject 2 captured by the porous filter 3, the light from the light source is scattered so that the contours of the pores in the porous filter 3 are not visible.
本発明における半透明スライドガラス５としては、全光
線透過率が３０％以上、平行光線透過率が６％以下、ヘ
イズ度が８５％以上であることが重要である。また、全
光線透過率は４５％以上、平行光線透過率は３．３％以
上、ヘイズ度は９０％以上であることが好ましい。これ
らの条件は、多孔性フィルター３の孔の輪郭を見えなく
するのに必要な光の散乱と高倍率での観察がしやすい視
界の明るさをえるためのものである。これらの範囲を外
れると、孔の輪郭が被検体２と重なって見えやすくなっ
たり、視界が暗くなったりする。It is important that the translucent slide glass 5 in the present invention has a total light transmittance of 30% or more, a parallel light transmittance of 6% or less, and a haze degree of 85% or more. Further, it is preferable that the total light transmittance is 45% or more, the parallel light transmittance is 3.3% or more, and the haze degree is 90% or more. These conditions are intended to achieve light scattering necessary to make the contours of the pores of the porous filter 3 invisible and to obtain a brightness of view that facilitates observation at high magnification. Outside these ranges, the outline of the hole overlaps with the subject 2, making it easier to see, or the field of vision becomes darker.
全光線透過率が上記３０％以上であることは、高倍率で
も観察しやすい視界の明るさを得るためのもので、視界
の明るさを得ることからすれば、高い値のものほど好ま
しいといえる。しかし、現実的にはこれを高くするほど
上記平行光線透過率及びヘイズ度が得にくくなるので、
どの程度まで全光線透過率の高いものとするかは、得ら
れる平行光線透過率とヘイズ度との関係から選択すれば
よい。現在、上記平行光線透過率及びヘイズ度を同時に
満足しようとすると、その上限は８０％程度である。ま
た、現在の技術で上記平行光線透過率とヘイズ度を同時
に満足させやすく、観察しやすい明るさが得られる点か
らすると 全光線透過率は４５〜５５％であることが好
ましい。The total light transmittance of 30% or more is to obtain a bright field of view that is easy to observe even at high magnification, and from the viewpoint of obtaining a bright field of view, a higher value is preferable. . However, in reality, the higher this value is, the more difficult it becomes to obtain the above-mentioned parallel light transmittance and haze degree.
The degree to which the total light transmittance is to be high may be selected based on the relationship between the obtained parallel light transmittance and the degree of haze. Currently, when attempting to simultaneously satisfy the above parallel light transmittance and haze degree, the upper limit is about 80%. Further, the total light transmittance is preferably 45 to 55% because current technology can easily satisfy the parallel light transmittance and haze degree at the same time and provide brightness for easy observation.
平行光線透過率が６％以下であることは、多孔性フィル
ター３の孔を見えに（くするためのもので、この観点か
らすれば低い値のものほど好ましいといえる。しかし、
現実的にはこれを低くするほど前記全光線透過率が得に
くくなるので、どの程度まで平行光線透過率の低いもの
とするかは、得られる全光線透過率との関係から選択す
ればよい。現在、前記全光線透過率を同時に満足しよう
とすると、その下限は１．５％程度である。また、現在
の技術で前記全光線透過率を同時に満足させやすく、孔
も見えにくい点からすると、平行光線透過率は２．３〜
３．３％であることが好ましい。The parallel light transmittance of 6% or less is intended to make the pores of the porous filter 3 more visible, and from this point of view, a lower value is more preferable. However,
In reality, the lower the parallel light transmittance is, the more difficult it becomes to obtain the total light transmittance, so the degree to which the parallel light transmittance should be made low may be selected based on the relationship with the obtained total light transmittance. Currently, when trying to satisfy the above-mentioned total light transmittance at the same time, the lower limit is about 1.5%. In addition, considering that it is easy to simultaneously satisfy the above-mentioned total light transmittance with the current technology and the holes are difficult to see, the parallel light transmittance is 2.3~
Preferably it is 3.3%.
ヘイズ度が８５％以上であることは、やはり多孔性フィ
ルター３の孔を見えに（くするためのもので、この観点
からすれば９０％以上の高い値のものほど好ましいとい
える。しかし、現実的にはこれを高くするほど前記全光
線透過率が得にくくなるので、どの程度までヘイズ度が
高いものとするかは、得られる全光線透過率との関係か
ら選択すればよい。現在、前記全光線透過率を同時に満
足しようとすると、その上限は９６％程度である。The haze degree of 85% or more is to make the pores of the porous filter 3 more visible, and from this point of view, a higher value of 90% or more is more preferable.However, in reality Generally speaking, the higher the haze is, the more difficult it becomes to obtain the total light transmittance, so how high the haze degree should be should be selected based on the relationship with the obtained total light transmittance. If the total light transmittance is to be satisfied at the same time, the upper limit is about 96%.
ヘイズ度が８５％以上であることは、多孔性フィルター
３の孔を見えに（くするためのものである点、前記平行
光線透過率−と同じであるが、本発明において特に重要
な条件であま。即ち、平行光線透過率が前記範囲内であ
っても比較的高く、また全光線透過率も比較的高い場合
、多孔性フィルター３の孔が見える場合があるが、ヘイ
ズ度が８５％以上であれば、顕微鏡光源の照射光線量を
絞ることで、視界を過剰に暗くすることなくこれを見え
なくすることができる。孔の輪郭を見えにくくするため
に、従来多孔性フィルター３の屈折率と同等な屈折率を
持つ液体を多孔性フィルター３の孔に流し込むという試
みがある。これのみでは効果が不十分であるが、この方
式を採用した場合、ヘイズ度が８５％以上であれば、よ
り明るい視界で孔の輪郭を見えなくすることができる。The haze degree of 85% or more is to make the pores of the porous filter 3 visible, which is the same as the above-mentioned parallel light transmittance, but it is a particularly important condition in the present invention. Ama: In other words, when the parallel light transmittance is relatively high even within the above range and the total light transmittance is also relatively high, the pores of the porous filter 3 may be visible, but the haze degree is 85% or more. If so, by narrowing down the amount of light irradiated by the microscope light source, it is possible to make this invisible without making the field of view excessively dark.In order to make it difficult to see the outline of the pores, the refractive index of the conventional porous filter 3 There is an attempt to pour a liquid with a refractive index equivalent to that into the pores of the porous filter 3. Although this method alone is insufficiently effective, if this method is adopted, and the haze degree is 85% or more, The brighter field of view makes it possible to hide the outline of the hole.
該半透明スライドガラス５は、アクリル樹脂からなる。The translucent slide glass 5 is made of acrylic resin.
本樹脂は、光学レンズ、眼鏡レンズ、コンタクトレンズ
等に用いられているように、光学的用途に適しており、
メチルメタクリレート（以後ＭＭＡと略称）を主体とし
た樹脂で、ポリメチルメタクリレート（以後ＰＭＭＡと
略称）、ＭＭＡとアルキルアクリレート共重合体（Ｃｏ
（ＭＭＡ−ＡＡ） 、ＭＭＡ−無水マレイン酸−ス
チレン三元系共重合体（Ｃｏ （ＭＭＡ−ＭＡＨ−３
ｔ））、ＭＭＡ−メタクリルアミド共重合体（Ｃｏ（Ｍ
ＭＡ−Ｍ Ａ Ａｍ１ｄｅ） ）等で、本樹脂に拡散剤
を混合復配向させた樹脂による光拡散層６を透明板７の
少なくとも片面に設けたものが好ましい。このような半
透明スライドガラス５を製造すると、前記条件を満たす
半透明スライドガラスを安価に容易に量産出来ると共に
、機械的特性、表面平滑性、耐薬品性に優れた光拡散層
６によって必要な光の拡散性能を付与することが容易と
なる。This resin is suitable for optical applications such as optical lenses, eyeglass lenses, contact lenses, etc.
A resin mainly composed of methyl methacrylate (hereinafter abbreviated as MMA), polymethyl methacrylate (hereinafter abbreviated as PMMA), MMA and alkyl acrylate copolymer (Co
(MMA-AA), MMA-maleic anhydride-styrene ternary copolymer (Co (MMA-MAH-3
t)), MMA-methacrylamide copolymer (Co(M
It is preferable to provide a light-diffusing layer 6 on at least one side of the transparent plate 7, which is made of a resin such as MA-MA Am1de) etc., which is made by mixing and reorienting a diffusing agent with this resin. By manufacturing such a translucent slide glass 5, it is possible to easily mass-produce translucent slide glasses that meet the above conditions at a low cost, and the light diffusing layer 6, which has excellent mechanical properties, surface smoothness, and chemical resistance, can meet the necessary requirements. It becomes easy to impart light diffusion performance.
該拡散剤としては、請求の範囲に示すとうりシリコーン
樹脂の球状粒子であり、その数平均粒子径が０．３〜４
μｍであることが好ましく、特に０．８〜２．０μｍで
あることが好ましい。The diffusing agent is spherical particles of silicone resin as shown in the claims, and has a number average particle diameter of 0.3 to 4.
It is preferably μm, particularly preferably 0.8 to 2.0 μm.
この範囲のシリコーン球状粒子と、アクリル樹脂の組合
せにより全光線透過率、平行光線透過率及びヘイズ度の
バランスをとりやすくなる。また、シリコーン樹脂球状
粒子の数平均粒子径が細かくなるほど全光線透過率は小
さくなり、大きくなるほど大となる傾向にあり、全光線
透過率、平行光線透過率及びヘイズ度を、この数平均粒
子径と共に後述するシリコーン樹脂球状粒子の量を選択
することにより、自由にコントロールが出来る。The combination of silicone spherical particles in this range and acrylic resin makes it easy to balance total light transmittance, parallel light transmittance, and haze degree. In addition, the smaller the number average particle diameter of silicone resin spherical particles, the smaller the total light transmittance, and the larger the number, the higher the total light transmittance.The total light transmittance, parallel light transmittance, and haze degree are It can be freely controlled by selecting the amount of silicone resin spherical particles, which will be described later.
上記シリコーン樹脂球状粒子の数平均粒子径は、次の測
定方法によるものをいう。The number average particle diameter of the silicone resin spherical particles is determined by the following measurement method.
測定装置：遠心式自動粒度分布測定装置（パティクルア
ナライザー）
型式： ＣＡＰＡ−５００型
測定方式：高速遠心沈陳法と自然沈障法を採用した光透
過式液相沈降粒度分布測定
法により算出
分散媒体：界面活性剤水溶液
分散条件：超音波分散
シリコーン樹脂球状粒子のアクリル樹脂への添加量は、
アクリル樹脂１００重量部に対して５〜３５重量部であ
ることが好ましい。この添加量が５重量部未満であると
、前述の光学的条件を満たす上で、光拡散層の厚さが、
１．５団以上必要となり、スライドガラスの厚みが大き
すぎてしまう。Measuring device: Centrifugal automatic particle size distribution measuring device (particle analyzer) Model: CAPA-500 Measuring method: Dispersion calculated by light transmission liquid phase sedimentation particle size distribution measuring method that uses high-speed centrifugal sedimentation method and natural sedimentation method Medium: Surfactant aqueous solution Dispersion conditions: Ultrasonic dispersion Silicone resin The amount of spherical particles added to the acrylic resin is
The amount is preferably 5 to 35 parts by weight based on 100 parts by weight of the acrylic resin. If the amount added is less than 5 parts by weight, the thickness of the light diffusing layer will be
More than 1.5 groups are required, and the thickness of the slide glass becomes too large.
また、逆に添加量が多すぎると光拡散層の樹脂の成形性
（押出性等）、物性（衝撃性等）に問題を生じる。On the other hand, if the amount added is too large, problems will arise in the moldability (extrudability, etc.) and physical properties (impact resistance, etc.) of the resin of the light-diffusing layer.
光拡散層６を配設する透明基板７としては、般のスライ
ドガラスとして用いられているものと同様なソーダーガ
ラス板でもよいが、合成樹脂板等地の板材でもよい。特
にホウケイ酸ガラス板は、紫外線透過率がよく、蛍光顕
微鏡観察に対しても有効であるので好ましい。The transparent substrate 7 on which the light diffusion layer 6 is disposed may be a soda glass plate similar to that used as a general slide glass, or may be a synthetic resin plate or the like. In particular, a borosilicate glass plate is preferable because it has good ultraviolet transmittance and is also effective for fluorescence microscopic observation.
シリコーン球状粒子を混合後配向したアクリル樹脂を用
いて光拡散層６を形成する方法の一例を以下に記述する
。An example of a method for forming the light diffusion layer 6 using an acrylic resin in which silicone spherical particles are mixed and oriented will be described below.
まずシリコーン球状粒子５〜３５重量部とアクリル樹脂
１００重量部とを通常使用される押出成形法により混合
し、ペレタイズした後、再び押出成形法により１ｍｍ〜
１５ｍｍの厚さのシートに成形する。その後、このシー
トを圧縮ダイ内で圧縮して面積比で２〜１０倍に配向成
形（圧縮成形法、特開昭６０−１４９４２０号公報参照
）し所定寸法に裁断光拡散層６を成形する。その後透明
基板７（第１〜４図）あるいは支持板７−２（第５図）
に接着法、射出成形法等で、光拡散層６を有する半透明
スライドガラス５を得ることができる。First, 5 to 35 parts by weight of silicone spherical particles and 100 parts by weight of acrylic resin are mixed by a commonly used extrusion molding method, pelletized, and then extruded again to a size of 1 mm to 1 mm.
Form into a 15 mm thick sheet. Thereafter, this sheet is compressed in a compression die and oriented and molded to have an area ratio of 2 to 10 times (compression molding method, see Japanese Patent Application Laid-open No. 149420/1983) to form a cut light diffusing layer 6 to a predetermined size. After that, the transparent substrate 7 (Figs. 1 to 4) or the support plate 7-2 (Fig. 5)
The translucent slide glass 5 having the light diffusing layer 6 can be obtained by a bonding method, an injection molding method, or the like.
この圧縮配同法によって成形した光拡散層６は機械的特
性、耐薬品性等に優れた特性を有し、スライドガラスと
しての必要特性を満足するものである。The light diffusing layer 6 formed by this compression distribution method has excellent mechanical properties, chemical resistance, etc., and satisfies the properties required for a glass slide.
以下の材料及び方法により拡散大人アクリル樹脂からな
る光拡散層６を有する半透明スライドガラス５を作成し
た。以下拡散剤の重量部はＰＭＭＡ樹脂１００重量部に
対する量である。A translucent slide glass 5 having a light diffusion layer 6 made of a diffusion adult acrylic resin was prepared using the following materials and method. Hereinafter, parts by weight of the diffusing agent are based on 100 parts by weight of the PMMA resin.
（１）透明基板７
透明基板Ａニホウケイ酸ガラス板（８８５μｍ）透明基
板Ｂニホウケイ酸ガラス板（１０１０μｌ１１）大きさ
は三者とも２６Ｘ７６ｍｍとした。(1) Transparent substrate 7 Transparent substrate A diborosilicate glass plate (885 μm) Transparent substrate B diborosilicate glass plate (1010 μl 11) The size of all three was 26×76 mm.
（以下余白） （２）光拡散層６ 光拡散層Ｃ：拡拡散大入配向超高分子量ＭＭＡ。(Margin below) (2) Light diffusion layer 6 Light diffusion layer C: ultra-high molecular weight MMA with wide diffusion and high input orientation.
拡散剤：シリコーン樹脂球状粒子 （粒子径：２μｍ）１５重量部。Diffusing agent: silicone resin spherical particles (Particle size: 2 μm) 15 parts by weight.
セルキャスト法で成形した厚さ
２．４ ｍｍのシートを、圧縮ダイ温度１５０°Ｃにて
圧縮２軸配向成形に
より、面積比で４倍に２軸配向し
厚さ０．６ｍｍのシートを得、２６ Ｘ ７６胴に切削
加工した。A sheet with a thickness of 2.4 mm formed by the cell casting method was biaxially oriented by 4 times the area ratio by compression biaxial orientation molding at a compression die temperature of 150°C to obtain a sheet with a thickness of 0.6 mm. , machined into a 26 x 76 barrel.
光拡散層Ｄ：拡拡散大入配向ＭＭＡ。Light diffusion layer D: MMA with high diffusion and high input orientation.
拡散剤：シリコーン樹脂球状粒子 （粒子径＝２μｍ）２０重量部。Diffusing agent: silicone resin spherical particles (Particle size = 2 μm) 20 parts by weight.
拡散割入汎用ＰＭＭＡ （押出成形 法により拡散剤配合）製シート （押出成形法によりシート成形） を、圧縮ダイ温度１５０°Ｃにて圧 縮２軸配開成形により面積比で４ 倍に２軸配向し厚さ０．６ｍｍのシ トを得、２６ Ｘ ７６ｍｍに切削加工した。Diffusion-interrupted general-purpose PMMA (extrusion molding Sheet made with diffuser compounded according to the law (Sheet molding using extrusion molding method) is compressed at a compression die temperature of 150°C. The area ratio is 4 due to shrinkage biaxial expansion molding. A sheet with double biaxial orientation and a thickness of 0.6 mm. A piece was obtained and cut into a size of 26 x 76 mm.
光拡散層Ｅ：拡拡散大入配向ＭＭＡ。Light diffusion layer E: MMA with high diffusion orientation.
拡散剤：シリコーン樹脂球状粒子 （粒子径＝２μｍ）１０重量部。Diffusing agent: silicone resin spherical particles (Particle size = 2 μm) 10 parts by weight.
拡散剤人混用ＰＭＭＡ （押出成形 法により拡散剤配合）類シート （押出成形法によりシート成形） を、圧縮ダイ温度１５０°Ｃにて圧縮 ２軸配向成形により面積比で４倍 に２軸配向し厚さ０．６ｍｍのシート を得、２６ Ｘ ７６閣に切削加工した。PMMA for mixing with diffusion agent (extrusion molding) Diffusing agent blended according to law) type sheet (Sheet molding using extrusion molding method) is compressed at a compression die temperature of 150°C. 4 times the area ratio due to biaxial orientation molding 0.6mm thick sheet with biaxial orientation was obtained and cut into a size of 26 x 76.
光拡散層Ｆ：拡拡散大入配向ＭＭＡ。Light diffusion layer F: MMA with high diffusion orientation.
拡散剤：シリコーン樹脂球状粒子 （粒子径：２μｍ）２５重量部。Diffusing agent: silicone resin spherical particles (Particle size: 2 μm) 25 parts by weight.
拡散剤人混用ＰＭＭＡ （押出成形 法により拡散剤配合）類シート （押出成形法によりシート成形） を、圧縮ダイ温度１５０°Ｃにて圧縮 ２軸配向成形により面積比で４倍 に２軸配向し厚さ０．６ｍｍのシート を得、２６ Ｘ ７６ｍｍに切削加工した。PMMA for mixing with diffusion agent (extrusion molding) Diffusing agent blended according to law) type sheet (Sheet molding using extrusion molding method) is compressed at a compression die temperature of 150°C. 4 times the area ratio due to biaxial orientation molding Biaxially oriented sheet with a thickness of 0.6 mm was obtained and cut into a size of 26 x 76 mm.
光拡散層Ｇ：拡拡散大入配向ＭＭＡ。Light diffusion layer G: MMA with wide diffusion and high input orientation.
拡散剤：シリコーン樹脂球状粒子 （粒子径：２μｍ）３０重量部。Diffusing agent: silicone resin spherical particles (Particle size: 2 μm) 30 parts by weight.
拡散剤人混用ＰＭＭＡ （押出成形 法により拡散剤配合）類シート （押出成形法によりシート成形） を、圧縮ダイ温度１５０°Ｃにて圧 縮２軸配向成形により面積比で４ 倍に２軸配向し厚さ０．６ｎｔｍのシ トを得、２６ Ｘ ７６ｍｍに切削加工した。PMMA for mixing with diffusion agent (extrusion molding) Diffusing agent blended according to law) type sheet (Sheet molding using extrusion molding method) is compressed at a compression die temperature of 150°C. The area ratio is 4 due to contraction biaxial orientation molding. A sheet with double biaxial orientation and a thickness of 0.6 ntm. A piece was obtained and cut into a size of 26 x 76 mm.
光拡散層Ｈ：拡拡散大人無配向ＭＭＡ。Light diffusion layer H: Diffusion adult non-oriented MMA.
拡散剤：シリコーン樹脂球状粒子 （粒子径：２μｍ）１０重量部。Diffusing agent: silicone resin spherical particles (Particle size: 2 μm) 10 parts by weight.
拡散剤人混用ＰＭＭＡ （押出成形 法により拡散剤配合）類シート （押出成形法によりシート成形） を、２６ Ｘ ７６薗に切削加工した。PMMA for mixing with diffusion agent (extrusion molding) Diffusing agent blended according to law) type sheet (Sheet molding using extrusion molding method) was cut into a size of 26 x 76 mm.
（３）作成方法
上記の透明基板７二者と光拡散層６各々を両面テープに
チバン製）で接着組合せ、６種類の半透明スライドガラ
ス５を作成した。(3) Production method The two transparent substrates 7 and the light diffusion layer 6 were adhesively combined with double-sided tape (manufactured by Chiban Co., Ltd.) to produce six types of translucent glass slides 5.
上記によって得た半透明スライドガラス５の全光線透過
率、平行光線透過率、ヘイズ度を下記の装置及び方法で
測定した。The total light transmittance, parallel light transmittance, and degree of haze of the translucent slide glass 5 obtained above were measured using the following apparatus and method.
光学特性測定：東京重色製、デイジタルヘーズメーター
ＴＣ−Ｈｕｔ型
上記の測定の結果を第１表に示す。Optical property measurement: Digital haze meter TC-Hut type manufactured by Tokyo Juishiki Co., Ltd. The results of the above measurements are shown in Table 1.
一方、下記の材料及び方法により、枠体付多孔性フィル
ター８を作成した。On the other hand, a porous filter with a frame 8 was created using the materials and method described below.
（１）枠体４
厚さ１５０ ｕｍで、２５Ｘ２９ｍｍの角を落とした方
形のステンレススチール板の中央部に直径２１ｍｍの円
孔を打ち抜いたものを使用した。(1) Frame 4 A rectangular stainless steel plate measuring 25 x 29 mm with rounded corners and having a thickness of 150 um was used, with a circular hole 21 mm in diameter punched in the center.
（２）多孔性フィルター３
厚さ７μ糟のポリカーボネイトフィルムに直径３μ麟の
直孔を形成したもの（ジェネラルエレクトリック社製の
「ニュークリポアフィルター」）を用いた。(2) Porous Filter 3 A polycarbonate film with a thickness of 7 μm and straight pores of 3 μm in diameter formed therein (“Nucrypore Filter” manufactured by General Electric Company) was used.
（３）作成方法
枠体４にホットメルトフィルムを押圧加熱して張り付け
（１００〜１６０°Ｃの温度）、その上に多孔性フィル
ター３を載せて、再度押圧加熱（１００〜１６０°Ｃの
温度）することで両者を接合して、枠体付多孔性フィル
ター８を作成した。(3) Creation method Press and heat a hot melt film to the frame 4 (at a temperature of 100 to 160°C), place the porous filter 3 on top of it, and press and heat it again (at a temperature of 100 to 160°C). ), the two were joined together to create a porous filter with frame 8.
上記によって得た枠体付多孔性フィルター８と、半透明
スライドガラス５を用い、次のようにして細胞標本を作
成した。Using the frame-attached porous filter 8 obtained above and the translucent slide glass 5, a cell specimen was prepared in the following manner.
まず、生体組織をカミソリで薄く切片にし、培養液に入
れ、注射針を付けた注射器に吸い込み、これを培養液中
に急速に押し出すことで単細胞を得た。First, living tissue was cut into thin sections with a razor, placed in culture medium, sucked into a syringe with a needle attached, and rapidly extruded into the culture medium to obtain single cells.
ついで、口過器の上に作成した枠体付多孔性フィルター
８を載せ、上記によって得た培養液中の単細胞を口過し
た。これによって枠体付多孔性フィルター８に捕捉され
た細胞に、多孔性フィルタ３で固定化、染色及び洗浄処
理を設し、上記の各半透明スライドガラス５の上に載せ
、顕微鏡により、５０倍、１００倍、２００倍、１００
０倍に拡大して各々観察を行った。Next, the prepared porous filter 8 with a frame was placed on the mouth strainer, and the single cells in the culture solution obtained above were passed through the mouth. As a result, the cells captured on the frame-attached porous filter 8 are fixed with the porous filter 3, stained and washed, placed on each of the above-mentioned translucent slide glasses 5, and examined with a microscope at 50x magnification. , 100 times, 200 times, 100
Each observation was made with 0x magnification.
その結果、細胞標本作成が極めて容易で、作業性に優れ
ていることがｌ＋１！認された。また実施例（光拡散層
Ｃ−Ｃ）及び比較例（光拡散層Ｈ）のいずれの半透明ス
ライドガラス５においても多孔性フィルター３の孔が見
えず、いずれの倍率においても鮮明に観察することが出
来た。As a result, it is extremely easy to prepare cell specimens and has excellent workability! It has been certified. In addition, the pores of the porous filter 3 are not visible in any of the translucent slide glasses 5 of the example (light diffusion layer C-C) and the comparative example (light diffusion layer H), and the observation can be made clearly at any magnification. was completed.
また厚さ１．５ｍｍの透明スライドガラス７を用いた場
合、観察に際し多孔性フィルター３の孔が細胞と重複し
て見え、詳細な観察は出来なかった。Furthermore, when a transparent slide glass 7 with a thickness of 1.5 mm was used, the pores of the porous filter 3 appeared to overlap with the cells during observation, making detailed observation impossible.
なお、この顕微鏡観察用細胞標本を作成するためには、
洗浄用等エチルアルコール等各種の有機溶剤を使用する
場合が多い。そこで、この有機溶剤特性（耐薬品性）を
調べるため、光拡散層Ｃ〜Ｈのエチルアルコールへのじ
ゃふ漬はテストを行った結果、配向した光拡散層Ｃ−Ｇ
では何の変化も無く良好であったが、無配向の光拡散層
Ｈではひび割れが激しくスライドガラスの光拡散層とし
ての使用が困難であることがわかった。In addition, in order to create this cell specimen for microscopic observation,
Various organic solvents such as ethyl alcohol are often used for cleaning purposes. Therefore, in order to investigate this organic solvent property (chemical resistance), we conducted a test of dipping the light diffusion layers C to H in ethyl alcohol.
However, it was found that the non-oriented light diffusing layer H had severe cracking and was difficult to use as a light diffusing layer for slide glass.
第 １
表
〔発明の効果］
本発明は以上説明したとうり、シリコーン球状粒子を含
有する、拡散割入配向アクリル樹脂による光拡散層をス
ライドガラスに適用することにより、機械的特性、耐薬
品性等にすくれた、効果的な半透明スライドガラス５を
安価・経済的に製造することが出来、多孔性フィルター
を用いた顕微鏡検査システムを真に実用的なシステムと
することが出来、当該システムの普及を通じて顕微鏡検
査の迅速化及び確実化を可能とするものである。Table 1 [Effects of the Invention] As explained above, the present invention improves mechanical properties and chemical resistance by applying a light diffusion layer made of a diffusion cracked oriented acrylic resin containing silicone spherical particles to a slide glass. It is possible to manufacture an effective semi-transparent slide glass 5 with a low profile, and to make a microscopic examination system using a porous filter into a truly practical system. Through the widespread use of this technology, microscopic examinations can be made faster and more reliable.
第１図は本発明の、拡散割入配向アクリル樹脂による光
拡散層６を有する半透明スライドガラス５と被検体２を
装着させた枠体付多孔性フィルタ８との組合せ図であり
、第２図は全体が拡散割入配向アクリル樹脂による光拡
散層６で作られた半透明スライドガラス５である。第３
．４図は光拡散層６が円形、四角形である半透明スライ
ドガラスであり、第５図は、光拡散層６が二つ設けられ
た半透明スライドガラスである。第６図は、光拡散層６
に支持板７−２を適用した半透明スライドガラスである
。
１−−−−−一カバーガラス
２−・・−被検体
３−・−−−−一多孔性フイルター
４−−−−−−−枠体
５−−−一半透明スライドガラス
６−−−−−光拡散層（拡散割入配向アクリル樹脂部）
７−−−−−−透明基板（透明スライドガラス）７−２
−−−−一支持板
８−・−枠体付多孔性フィルター（フィルタシステム）FIG. 1 is a combination diagram of a translucent slide glass 5 having a light diffusion layer 6 made of a diffusion-cut oriented acrylic resin and a frame-attached porous filter 8 on which a subject 2 is mounted, according to the present invention. The figure shows a translucent glass slide 5 made entirely of a light diffusing layer 6 made of a diffusive and oriented acrylic resin. Third
．． 4 shows a translucent slide glass in which the light diffusion layer 6 is circular or square, and FIG. 5 shows a translucent slide glass in which two light diffusion layers 6 are provided. FIG. 6 shows the light diffusion layer 6
This is a translucent slide glass to which a support plate 7-2 is applied. 1 ------ One cover glass 2- ... - Incident 3- ・ ---- One porous filter 4 ----------------------al --Light diffusion layer (diffusion cut orientation acrylic resin part) 7 --- Transparent substrate (transparent slide glass) 7-2
--- Support plate 8 -- Porous filter with frame (filter system)
Claims (1)
状粒子を含有する、配向したアクリル樹脂からなり、全
光線透過率が３０％以上、平行光線透過率が６％以下、
ヘイズ度が８５％以上である光拡散層を有する半透明ス
ライドガラス。1. Made of oriented acrylic resin containing spherical particles of silicone resin with a number average particle diameter of 0.3 to 4 μm, with a total light transmittance of 30% or more and a parallel light transmittance of 6% or less,
A translucent slide glass having a light diffusion layer with a haze degree of 85% or more.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP20821090A JPH0495914A (en) | 1990-08-08 | 1990-08-08 | Translucent slide glass |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP20821090A JPH0495914A (en) | 1990-08-08 | 1990-08-08 | Translucent slide glass |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0495914A true JPH0495914A (en) | 1992-03-27 |
Family
ID=16552497
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP20821090A Pending JPH0495914A (en) | 1990-08-08 | 1990-08-08 | Translucent slide glass |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0495914A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011004714A1 (en) * | 2009-07-06 | 2011-01-13 | 株式会社ニコン | Microscope |
-
1990
- 1990-08-08 JP JP20821090A patent/JPH0495914A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011004714A1 (en) * | 2009-07-06 | 2011-01-13 | 株式会社ニコン | Microscope |
| JP5472831B2 (en) * | 2009-07-06 | 2014-04-16 | 株式会社ニコン | Microscope equipment |
| US8922886B2 (en) | 2009-07-06 | 2014-12-30 | Nikon Corporation | Microscope apparatus having surface light emitter with specific positioning |
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