JPH0494702A - Chromatographic separating apparatus using super-critical fluid - Google Patents
Chromatographic separating apparatus using super-critical fluidInfo
- Publication number
- JPH0494702A JPH0494702A JP2214050A JP21405090A JPH0494702A JP H0494702 A JPH0494702 A JP H0494702A JP 2214050 A JP2214050 A JP 2214050A JP 21405090 A JP21405090 A JP 21405090A JP H0494702 A JPH0494702 A JP H0494702A
- Authority
- JP
- Japan
- Prior art keywords
- supercritical fluid
- extract
- valve
- extraction tank
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000012530 fluid Substances 0.000 title claims abstract description 43
- 239000000284 extract Substances 0.000 claims abstract description 41
- 238000000605 extraction Methods 0.000 claims description 44
- 238000004587 chromatography analysis Methods 0.000 claims description 27
- 238000000926 separation method Methods 0.000 claims description 23
- 238000004808 supercritical fluid chromatography Methods 0.000 claims description 15
- 239000000126 substance Substances 0.000 claims description 4
- 229920006395 saturated elastomer Polymers 0.000 description 7
- 238000000034 method Methods 0.000 description 5
- 239000007788 liquid Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 238000013375 chromatographic separation Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は超臨界流体クロマト分離装置に係り、特に分離
精製技術の分野で用しハられる超臨界流体クロマト分離
装置に関する。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a supercritical fluid chromatography separation device, and particularly to a supercritical fluid chromatography separation device used in the field of separation and purification technology.
最近、バイオテクノロジーを利用した高付加価値物質の
生産が盛んに行われている。これらの物質は、その性質
上、培養等により微量でしか生産できないので、高純度
化することが困難である。Recently, the production of high value-added substances using biotechnology has been actively carried out. Due to their nature, these substances can only be produced in trace amounts by culturing or the like, so it is difficult to achieve high purity.
また、前記バイオ生産物は慎重な取り扱いが要求され、
熱や有機溶媒の影響で死活するものも多いので、バイオ
テクノロジーの技術、即ち蒸溜や抽出等の分離精製技術
をそのまま適用することが困難である。In addition, the bioproducts require careful handling;
Since many of them die due to the effects of heat and organic solvents, it is difficult to directly apply biotechnology techniques, such as separation and purification techniques such as distillation and extraction.
そこで、前述した不具合を解消する為に化学分析用とし
て使用されている超臨界流体クロマト分離装置を工業用
分離精製装置として使用する試みが提案されている。Therefore, in order to solve the above-mentioned problems, an attempt has been made to use a supercritical fluid chromatographic separation device used for chemical analysis as an industrial separation and purification device.
このような超臨界流体クロマト分離装置は第２図に示す
ように、ボンベ１０に充填された液体又は気体を高圧ポ
ンプ１２て加圧すると共に熱交換器１４て加温して超臨
界状態にし、この超臨界状＠：こなった超臨界流体を抽
出槽１６に導入する。As shown in FIG. 2, such a supercritical fluid chromatography separation device pressurizes a liquid or gas filled in a cylinder 10 with a high-pressure pump 12 and heats it with a heat exchanger 14 to bring it into a supercritical state. Supercritical state @: The purified supercritical fluid is introduced into the extraction tank 16.
前記抽出槽１６内には固体又は液体の試料が充填され、
前記超臨界流体がこの抽出槽１６に導入することにより
、前記試料から抽出物、即ちバイオ生産物の抽出が行わ
れる。The extraction tank 16 is filled with a solid or liquid sample,
By introducing the supercritical fluid into the extraction tank 16, an extract, that is, a bioproduct, is extracted from the sample.
そして、前記バイオ生産物が含有した超臨界流体は、そ
の一部がトラップループ１８にサンプリンクされた後、
クロマトグラフィカラム２０に導入する。そして、前記
クロマトグラフィカラム２０を通過することにより超臨
界流体からバイオ生産物が分離される。分離したバイオ
生産物は、検出器２２、背圧バルブ２４を介して分離槽
２６に送り込まれて取得される。Then, after a part of the supercritical fluid contained in the bioproduct is sampled and linked to the trap loop 18,
Chromatography column 20 is introduced. Then, the bioproduct is separated from the supercritical fluid by passing through the chromatography column 20. The separated bioproduct is sent to a separation tank 26 via a detector 22 and a back pressure valve 24 to be obtained.
しかしながら、従来の超臨界流体クロマト分離装置を分
取を目的として使用する場合、試料から抽出した抽出物
をクロマトグラフィカラム２０に全量導入することが要
求される。従って、従来の超臨界流体クロマト分離装置
では、抽出物の一部がトランプループ１８によって勺ン
プリンクされるので、分取を目的として使用することが
できないという欠点がある。However, when using a conventional supercritical fluid chromatographic separation device for the purpose of preparative separation, it is required to introduce the entire amount of the extract extracted from the sample into the chromatography column 20. Therefore, in the conventional supercritical fluid chromatography separation device, a part of the extract is linked by the transducer loop 18, so there is a drawback that it cannot be used for the purpose of preparative separation.
このような不具合を解消する方法として、トランプルー
プ１８を取り除いて抽出槽１４をクロマトグラフィカラ
ム２０に直結する方法がある。しかし、この方法では抽
出が行われている時間中抽出物がクロマトグラフィカラ
ム２０に導入されるので、導入中の抽出物濃度が刻々と
変化すると、クロマトグラフィカラム２０による分離効
率が悪化するという欠点がある。また、多量の超臨界流
体が必要になるという欠点もある。One way to solve this problem is to remove the Trump loop 18 and directly connect the extraction tank 14 to the chromatography column 20. However, in this method, the extract is introduced into the chromatography column 20 while the extraction is being performed, so if the concentration of the extract during introduction changes moment by moment, the separation efficiency by the chromatography column 20 deteriorates. . Another disadvantage is that a large amount of supercritical fluid is required.
本発明はこのような事情に鑑みてなされたもので、少量
の超臨界流体で抽出物を抽出することができ、クロマト
グラフィカラムに短時間で、且つ一定の濃度で抽出物を
導入することができる超臨界流体クロマト分離装置を提
供することを目的とする。The present invention was made in view of these circumstances, and it is possible to extract an extract with a small amount of supercritical fluid, and it is possible to introduce the extract into a chromatography column in a short time and at a constant concentration. The purpose of the present invention is to provide a supercritical fluid chromatography separation device.
本発明は、前記目的を達成する為に、試料（３７）が充
填された抽出槽（１６）に超臨界流体を導入して試料く
３７）から抽出物を抽出し、この抽出物が含有した超臨
界流体を連結系路を介してクロマトグラフィカラム（２
０）に通過させて前記抽出物を超臨界流体から分離する
超臨界流体クロマト分離装置に於いて、抽出槽（１６）
の容積を膨縮可能な容積可変装置く３２）と、連結系路
に設けられ、容積可変装置（３２）の動作と連動して開
閉するバルブ（４２）と、を備えたことを特徴とする。In order to achieve the above object, the present invention extracts an extract from the sample (37) by introducing a supercritical fluid into the extraction tank (16) filled with the sample (37), and extracts the extract from the sample (37). The supercritical fluid is transferred to the chromatography column (2
0) in a supercritical fluid chromatography separation device for separating the extract from a supercritical fluid, an extraction tank (16);
A variable volume device (32) capable of expanding and contracting the volume of the device (32), and a valve (42) that is provided in the connection path and opens and closes in conjunction with the operation of the variable volume device (32). .
本発明によれば、試料（３７）の抽出時に連結系路のバ
ルブ（４２）を閉じて超臨界流体を抽出槽（１６）に導
入する。これにより、少量の超臨界流体で抽出物を抽出
することができる。そして、容積可変装置（３２）を作
動すると同時に、前記バルブ（４２）を開放して抽出槽
（１６）の容積を縮小させ、抽出槽（１６）内の抽出物
を含有する前記超臨界流体を連結系路を介してクロマト
グラフィカラム（２０）に全量導入する。According to the present invention, when extracting the sample (37), the valve (42) of the connecting line is closed and the supercritical fluid is introduced into the extraction tank (16). This allows the extract to be extracted using a small amount of supercritical fluid. Then, at the same time as the volume variable device (32) is operated, the valve (42) is opened to reduce the volume of the extraction tank (16), thereby draining the supercritical fluid containing the extract in the extraction tank (16). The entire amount is introduced into the chromatography column (20) via the connecting line.
また、前記抽出槽（１６）に循環系路を設けて、試料の
抽出中ｉこ抽出槽（１６）に導入した超臨界流体を抽出
槽（１６）から吸引して抽出槽（１６）に再び供給する
ことにより、抽出物を短時間で抽出することができる。Further, a circulation system path is provided in the extraction tank (16) to suck the supercritical fluid introduced into the extraction tank (16) during sample extraction from the extraction tank (16) and return it to the extraction tank (16). By supplying the extract, the extract can be extracted in a short time.
以下添付図面に従って本発明に係る超臨界流体クロマト
分離装置の好ましい実施例を詳説する。Preferred embodiments of the supercritical fluid chromatography separation apparatus according to the present invention will be described in detail below with reference to the accompanying drawings.
第１図は本発明に伜る超臨界流体クロマト分離装置の実
施例が示され、第２図に示した従来例中と同−若しくは
類似の部材については、同一の符号を付して説明する。FIG. 1 shows an embodiment of a supercritical fluid chromatography separation device according to the present invention, and the same or similar members as in the conventional example shown in FIG. 2 will be described with the same reference numerals. .
第１図によれば、ボンベ１０は流路切換バルブ２８のバ
ルブ２８ｂ、２８ａを介して高圧ポンプ１２、熱交換器
１４に連結される。前記熱交換器１４は三方バルブ３０
のバルブ３０ａ、３０ｂを介して円筒状の抽出槽１６に
連通される。従って、前記ボンベ１０に充填された液体
又は気体は、高圧ポンプ１２により吸引されて加圧され
、更に熱交換器１４により加温されて超臨界流体となり
、この超臨界流体か三方バルブ３０のバルブ３０ａ、３
０ｂを開放することにより抽出槽１６に導入される。According to FIG. 1, the cylinder 10 is connected to the high pressure pump 12 and the heat exchanger 14 via valves 28b and 28a of the flow path switching valve 28. The heat exchanger 14 is a three-way valve 30
It is communicated with the cylindrical extraction tank 16 through valves 30a and 30b. Therefore, the liquid or gas filled in the cylinder 10 is sucked and pressurized by the high-pressure pump 12 and further heated by the heat exchanger 14 to become a supercritical fluid. 30a, 3
By opening 0b, it is introduced into the extraction tank 16.
前記槽１６内にはピストン３２が抽出槽１６の内壁に沿
って摺動自在に配置され、このピストン３２のピストン
ロッド３４がエアシリンダ３６に取付けられるっ従って
、前記エアシリンダ３６内にエアを供給すると、ピスト
ンロッド３４がエア圧に押されて上昇するので、ピスト
ン３２を図中二点鎖線で示す位置まで上昇させることが
できる。A piston 32 is disposed within the tank 16 so as to be able to freely slide along the inner wall of the extraction tank 16, and a piston rod 34 of the piston 32 is attached to an air cylinder 36, thereby supplying air into the air cylinder 36. Then, the piston rod 34 is pushed up by the air pressure, so that the piston 32 can be raised to the position shown by the two-dot chain line in the figure.
即ち、抽出槽１６の容積を縮小させることができる。ま
た、前記エアシリンダ３６内のエアを吸弓してピストン
３２を当初の位置に復帰させることにより、抽出槽１６
の容積を膨張させることができる。尚、前記ピストン３
２の上部には抽出用の試料３７が載置される。この試料
３７中から、抽出槽１６に導入される前記超臨界流体に
よって試料３７中の抽出物が抽出される。That is, the volume of the extraction tank 16 can be reduced. Also, by sucking the air in the air cylinder 36 and returning the piston 32 to its original position, the extraction tank 16
The volume of can be expanded. In addition, the piston 3
A sample 37 for extraction is placed on top of the sample 2 . The extract in the sample 37 is extracted from the sample 37 by the supercritical fluid introduced into the extraction tank 16.
前記抽出槽１６は、バルブ３８、三方バルブ４０のバル
ブ４０ｂ、４０ａ１背圧ノ−ルブ４２及びバルブ４４を
介してクロマトクラフイ力ラム２０に連結されろ。また
、前記三方ノリレブ４０のノ＼ルブ４０Ｃは、前述した
流路切換）＜ルブ２８のノールブ２８ｃ、２８ｄ及びバ
ルブ４６を介して前記背圧バルブ４２に連結される。更
に、前述した三方バルブ３０のバルブ３０ｃは、バルブ
４８を介してクロマトグラフィカラム２０に連結される
。The extraction tank 16 is connected to the chromatography power ram 20 through a valve 38, a three-way valve 40, a valve 40b, a back pressure knob 42 and a valve 44. Further, the knob 40C of the three-way knob 40 is connected to the back pressure valve 42 via the knobs 28c and 28d of the flow path switching valve 28 and the valve 46. Further, the valve 30c of the three-way valve 30 described above is connected to the chromatography column 20 via the valve 48.
前記クロマトグラフィカラム２０には、抽出物を含有し
た超臨界流体から抽出物を分離するカラムが充填される
。そして、前記クロマトクラフイ力ラム２０によって分
離された抽出物は、検出器２２及び背圧バルブ２４を介
して図示しない分離槽に送り込まれる。The chromatography column 20 is packed with a column that separates the extract from the supercritical fluid containing the extract. The extract separated by the chromatography ram 20 is sent to a separation tank (not shown) via a detector 22 and a back pressure valve 24.
次に、前記の如く構成された超臨界流体クロマト分離装
置の作用について説明する。Next, the operation of the supercritical fluid chromatography separator configured as described above will be explained.
先ず、試料３７の抽出工程について説明する。First, the extraction process of sample 37 will be explained.
先ず、ピストン３２を下降させて拍出槽１６の容積を膨
張させて最大容量にする。次に、流路切換バルブ２８の
バルブ２８Ｃ１２８ｄを連結して開放し、またバルブ２
８ａ、２８ｂを連結して開放してボンベ１０を高圧ポン
プ１２に連通させると共に、三方バルブ３０の３０ａ、
３０ｂを開放する。次いで、バルブ３８、三方バルブ４
０のバルブ４０ｂ、４０Ｃ及びバルブ４６を開放する。First, the piston 32 is lowered to expand the volume of the ejection tank 16 to its maximum capacity. Next, the valve 28C128d of the flow path switching valve 28 is connected and opened, and the valve 28C128d of the flow path switching valve 28 is connected and opened.
8a and 28b are connected and opened to connect the cylinder 10 to the high pressure pump 12, and the three-way valve 30, 30a,
30b is opened. Next, the valve 38 and the three-way valve 4
0 valves 40b, 40C and valve 46 are opened.
そして、これらのバルブの開放作業を終えると、高圧ポ
ンプ↓２及び熱交換器１４を作動して超臨界流体を抽出
槽１６に導入する。導入すると、抽出槽１６は背圧バル
ブ４２の作用によりその内圧が所定の圧力まで昇圧する
。これにより、前記試料３７の抽出が開始する。After the opening of these valves is completed, the high pressure pump ↓2 and the heat exchanger 14 are operated to introduce the supercritical fluid into the extraction tank 16. When introduced, the internal pressure of the extraction tank 16 is increased to a predetermined pressure by the action of the back pressure valve 42. As a result, extraction of the sample 37 is started.
次に、抽出槽１６の内圧が所定の圧力まで昇圧すると、
流路切換バルブ２８のバルブ２８ａ、２８Ｃを連結して
開放し、ボンベ１０からの液体又は気体の供給を停止さ
せる。そして、抽出槽１６内の超臨界流体中の抽出物濃
度が飽和に達するまで抽出槽１６に導入した前記超臨界
流体を循環させる。即ち、前記超臨界流体は、バルブ３
８、三方バルブのバルブ４０ｂ、４０Ｃを介して流路切
換バルブ２８のバルブ２８Ｃからバルブ２８ａに流動し
、そして高圧ポンプ１２、熱交換器１４及び三方バルブ
３０のバルブ３０ａ、３０ｂを介して抽出槽１６に循環
する。Next, when the internal pressure of the extraction tank 16 is increased to a predetermined pressure,
The valves 28a and 28C of the flow path switching valve 28 are connected and opened to stop the supply of liquid or gas from the cylinder 10. Then, the supercritical fluid introduced into the extraction tank 16 is circulated until the extract concentration in the supercritical fluid in the extraction tank 16 reaches saturation. That is, the supercritical fluid flows through the valve 3.
8. The flow flows from the valve 28C of the flow path switching valve 28 to the valve 28a through the valves 40b and 40C of the three-way valve, and flows into the extraction tank through the high-pressure pump 12, the heat exchanger 14, and the valves 30a and 30b of the three-way valve 30. Cycles to 16.
これにより、前記試料３７は、循環する超臨界カスに強
制的に接触するので、停滞した超臨界流体に試料を接触
させるようにした従来の抽出装置と比較して、短時間で
抽出物濃度を飽和させることができる。As a result, the sample 37 is forcibly brought into contact with the circulating supercritical scum, so the extract concentration can be increased in a short time compared to conventional extraction devices in which the sample is brought into contact with stagnant supercritical fluid. Can be saturated.
次いで、抽出物濃度が飽和に達すると、三方バルブ４０
の４０Ｃを閉じると共にバルブ４０ａを開放する。そし
て、ピストン３２を上昇させ、抽出槽１６内の超臨界流
体を、即ち抽出物濃度が飽和状態の超臨界流体を高圧雰
囲気て背圧バルブ４２、バルブ４４を介してクロマトグ
ラフィカラム２０に全量送り込む。この時、抽出物をク
ロマトグラフィカラム２０の上部のみに保持するよう操
作することにより、順次行う抽出工程を円滑に行うこと
ができる。Then, when the extract concentration reaches saturation, the three-way valve 40
40C and open the valve 40a. Then, the piston 32 is raised, and the entire amount of the supercritical fluid in the extraction tank 16, that is, the supercritical fluid with a saturated extract concentration is sent into the chromatography column 20 via the back pressure valve 42 and the valve 44 in a high pressure atmosphere. At this time, by holding the extract only in the upper part of the chromatography column 20, the sequential extraction steps can be performed smoothly.
そして、抽出槽１６の超臨界流体を全量送り込んだ後、
三方バルブ３０のバルブ３０ｂを閉じると共にバルブ３
０ｃを開放し、またこれと同時に流路切換バルブ２８の
バルブ２８ａとバルブ２８ｂを連通し、熱交換器１４か
らの超臨界流体を三方バルブ３０及びバルブ４８を介し
てクロマトグラフィカラム２０に導入する。これによっ
て、前記超臨界流体はクロマトグラフィの移動相の役割
を果たすことができるので、クロマトグラフィカラムに
送り込まれた前記抽出物をクロマトグラフィカラム２０
で円滑に、且つ安定して分離することができる。After feeding the entire amount of supercritical fluid into the extraction tank 16,
While closing the valve 30b of the three-way valve 30, the valve 3
0c is opened, and at the same time, valves 28a and 28b of the flow path switching valve 28 are brought into communication, and the supercritical fluid from the heat exchanger 14 is introduced into the chromatography column 20 via the three-way valve 30 and the valve 48. Accordingly, the supercritical fluid can serve as a mobile phase for chromatography, so that the extract sent to the chromatography column is transferred to the chromatography column 20.
can be separated smoothly and stably.
従って、本実施例によれば、抽出物を少量の超臨界流体
で短時間で飽和させることができると共に、抽出物をク
ロマトグラフィカラム２０に短時間で、且つ一定濃度で
送り込むことができる。これにより、超臨界流体クロマ
ト分離装置で分取を行うことができ、また超臨界流体り
ロマト分離装置の性能を低下させる原因を取り除くこと
ができる。Therefore, according to this embodiment, the extract can be saturated with a small amount of supercritical fluid in a short time, and the extract can be sent to the chromatography column 20 in a short time and at a constant concentration. Thereby, it is possible to carry out preparative separation using the supercritical fluid chromatographic separator, and it is also possible to eliminate causes of deterioration in the performance of the supercritical fluid chromatographic separator.
尚、本実施例では、試料３７からの抽出物の全成分をク
ロマトグラフィカラム２０に導入する方法を説明したが
、これに限られるものではなく、ポンプ１２、熱交換器
１４、抽出槽１６を結ぶ循環ライン中に吸着塔を設け、
抽出物中の特定成分のみを予め吸着させることにより、
クロマトグラフィカラム２０に導入する抽出物の絶対量
を減らし、クロマトグラフィカラム２０に対する負荷を
軽減して使用することも可能である。In this example, the method of introducing all the components of the extract from the sample 37 into the chromatography column 20 has been described, but the method is not limited to this, and the method is not limited to this. An adsorption tower is installed in the circulation line,
By pre-adsorbing only specific components in the extract,
It is also possible to reduce the absolute amount of extract introduced into the chromatography column 20 and reduce the load on the chromatography column 20.
以上説明したように本発明に係る超臨界流体クロマト分
離装置によれば、試料の抽出時に超臨界流体を導いて抽
出物が飽和状態になるまでバルブを閉じ、飽和状態にな
ると前記バルブを開放すると共に前記容積可変装置を作
動して抽出槽の容積を縮小させ、抽出物を含有する超臨
界流体をクロマトグラフィカラムに連結系路を介して全
量導入するようにしたので、抽出物を少量の超臨界流体
で飽和させることができ、更にこの抽出物を短時間で、
且つ一定濃度でクロマトグラフィカラムに導入すること
ができる。As explained above, according to the supercritical fluid chromatography separation device according to the present invention, when extracting a sample, the supercritical fluid is introduced and the valve is closed until the extract reaches a saturated state, and when the saturated state is reached, the valve is opened. At the same time, the volume variable device is activated to reduce the volume of the extraction tank, and the entire amount of the supercritical fluid containing the extract is introduced into the chromatography column via the connecting line. It can be saturated with fluid, and this extract can be saturated in a short time.
And it can be introduced into the chromatography column at a constant concentration.
１ 〕
第１図は本発明に係る超臨界流体クロマト分離装置の実
施例を示す説明図、第２図は従来の超臨界流体クロマト
分離装置の実施例を示す説明図である。
１６・・・抽出槽、
２０・・・クロマトグラフィカラム、
２８・流路切換バルブ、 ３０．４０・・三方バルブ、
３２・ ピストン、 ３６・・エアシリンダ、
４２・・・背圧バルブ。
出願人 日立プラント建設株式会社1] FIG. 1 is an explanatory diagram showing an embodiment of a supercritical fluid chromatography separation device according to the present invention, and FIG. 2 is an explanatory diagram showing an embodiment of a conventional supercritical fluid chromatography separation device. 16...Extraction tank, 20...Chromatography column, 28.Flow path switching valve, 30.40...Three-way valve,
32. Piston, 36. Air cylinder,
42... Back pressure valve. Applicant Hitachi Plant Construction Co., Ltd.
Claims (2)
試料から抽出物を抽出し、この抽出物が含有した超臨界
流体を連結系路を介してクロマトグラフィカラムに通過
させて前記抽出物を超臨界流体から分離する超臨界流体
クロマト分離装置に於いて、抽出槽の容積を膨縮可能な
容積可変装置と、連結系路に設けられ、容積可変装置の
動作と連動して開閉するバルブと、 を備えたことを特徴とする超臨界流体クロマト分離装置
。(1) A supercritical fluid is introduced into an extraction tank filled with a sample to extract an extract from the sample, and the supercritical fluid contained in the extract is passed through a chromatography column via a connection line to perform the extraction. In a supercritical fluid chromatography separation device that separates substances from a supercritical fluid, a volume variable device that can expand and contract the volume of an extraction tank is installed in a connecting path and opens and closes in conjunction with the operation of the volume variable device. A supercritical fluid chromatography separation device comprising: a valve;
ら吸引して抽出槽に再び供給する循環系路を設けたこと
を特徴とする請求項（１）記載の超臨界流体クロマト分
離装置。(2) The supercritical fluid chromatography separation device according to claim (1), further comprising a circulation system path for sucking the supercritical fluid introduced into the extraction tank from the extraction tank and supplying it again to the extraction tank. .
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2214050A JPH0494702A (en) | 1990-08-13 | 1990-08-13 | Chromatographic separating apparatus using super-critical fluid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2214050A JPH0494702A (en) | 1990-08-13 | 1990-08-13 | Chromatographic separating apparatus using super-critical fluid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0494702A true JPH0494702A (en) | 1992-03-26 |
Family
ID=16649439
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2214050A Pending JPH0494702A (en) | 1990-08-13 | 1990-08-13 | Chromatographic separating apparatus using super-critical fluid |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0494702A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06154507A (en) * | 1992-08-28 | 1994-06-03 | Kobe Steel Ltd | Weak polar useful component and method for extracting, separating and purifying the same |
| US5714924A (en) * | 1995-01-27 | 1998-02-03 | Tdk Corporation | Positive characteristic thermistor device |
| CN102764520A (en) * | 2011-05-04 | 2012-11-07 | 财团法人工业技术研究院 | High-pressure extraction equipment and extraction method thereof |
-
1990
- 1990-08-13 JP JP2214050A patent/JPH0494702A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06154507A (en) * | 1992-08-28 | 1994-06-03 | Kobe Steel Ltd | Weak polar useful component and method for extracting, separating and purifying the same |
| US5714924A (en) * | 1995-01-27 | 1998-02-03 | Tdk Corporation | Positive characteristic thermistor device |
| CN102764520A (en) * | 2011-05-04 | 2012-11-07 | 财团法人工业技术研究院 | High-pressure extraction equipment and extraction method thereof |
| US8828318B2 (en) | 2011-05-04 | 2014-09-09 | Industrial Technology Research Institute | High-pressure extraction apparatus and method |
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