JPH0483166A - Method for processing body fluid - Google Patents

Abstract

PURPOSE:To remove fibrinogen and fibrin contained in a body fluid so as to obtain blood serums at high yield without causing hemolysis by bringing the body fluid into contact with glass fiber of average diameter less than 9mu. CONSTITUTION:Blood is injected into an acrylic blood collecting tube having glass fiber stored therein and is made to go through a centrifugal separator so as to remove tangible components and fibrinogen in the blood, thereby obtaining blood serums with a content of fibrinogen and fibrin below 20mg/dl which amount fluctuates slightly. In this case, there is no need for the blood to be left for a long time so the blood can be promptly processed after being collected. The glass fiber whose average diameter is as small as less than 9mu or preferably less than 6mu is used. Also, long, middle or short glass fiber can be used which may be cut when in use. The glass fiber may be in the form of wool or glass paper and glass paper is preferable because it increases the yield of blood serums.

Description

DETAILED DESCRIPTION OF THE INVENTION (Industrial Field of Application) The present invention relates to a method for treating body fluids such as blood and ascites, particularly blood.

(Prior art) Blood is composed of blood, which is a pale yellow liquid component, and formed components, such as red blood cells, white blood cells, and platelets, and blood contains fibrinogen, a coagulation factor. .

When performing a blood test, it is necessary to remove fibrinogen contained in the blood, and the following methods are used to remove fibrinogen.

After the blood is collected, the blood is allowed to stand for about 30 minutes to precipitate fibrinogen contained in the blood as fibrin, and a blood clot made of blood solid components and fibrin is separated by centrifugation, and a supernatant liquid is collected.

Since fibrin and fibrinogen remain in the supernatant, stir them with a glass rod, etc. to remove them.
Collect serum.

(Problem to be solved by the invention) The conventional technology has the following problems.

(1) Since it is necessary to leave the blood for 30 minutes or more, the test takes time, causing problems in urgent tests.

(2) Stirring with a glass rod not only requires manpower, but also does not sufficiently remove fibrin and fibrinogen, which tend to remain in the serum and cause variations in the amount remaining.

When fibrin and fibrinogen remain in the serum,
Buoypurin precipitates during the inspection and may interfere with the inspection.

In particular, when an automatic testing device is used, the testing device may become clogged with fibrin and the test cannot be performed.

Furthermore, since there are variations in the remaining amounts of fibrin and fibrinogen, the accuracy of the test decreases.

(3) Serum yield is poor, and a large amount of blood is required to obtain a certain amount of serum.

The present invention solves the above-mentioned problems of the prior art, and provides a method for rapidly and sufficiently removing fibrin and fibrinogen from blood without requiring time or labor, and for obtaining serum at a high yield without causing hemolysis. is intended to provide.

Note that the method of the present invention can also be effectively used to remove fibrin and fibrinogen from ascites. (Hereinafter, blood and ascites are collectively referred to as body fluids.) (Means for Solving the Problems) In order to achieve the above object, in the present invention, by bringing body fluids into contact with glass fibers having an average diameter of 9W or less,
Removes fibrinogen and fibrin contained in body fluids.

Next, the present invention will be explained with a focus on blood processing.

In the present invention, glass fibers with an average diameter of 9 liters or less, preferably 6 fiL or less are used.

As the glass fibers of the present invention, long fibers, raw fibers, short fibers, or cut products thereof can be used.

The glass fibers may be in the form of wool or glass vapor, and glass vapor is particularly preferable, and the yield of serum can be increased.

Glass vapor refers to a paper-like material made of glass fiber II (monofilament), and its manufacturing method may be a wet method (paper making method) or a dry method.

A paper-like material can be produced by depositing glass fibers in a non-directional manner and bonding the fibers together.

When using a binder to firmly bind glass fibers together, it is necessary to use a binder that does not dissolve in body fluids and interfere with testing. For example, it is preferable to use a PVA-based binder. Ru.

The amount of binder used is preferably as small as possible (20 wt% or less, preferably 10 wt% or less) as long as the glass vapor has sufficient shape retention.

It is appropriate to determine the diameter and mixing ratio of the glass fibers so that the total surface area of the glass fibers in 1 gram of glass vapor is 0.1 m2 or more, preferably 0.25 m2 or more.

Furthermore, glass fibers with a low K and Na content, particularly those with a 20 content of 6 wt% or less, preferably 3 wt% or less, and a Na2O content of 18 wt%
It is preferable to use 12 wt% or less.

For example, 5iO266,7wt%, Ca06゜7w
t%, 01.4wt%, B2033-9wt%, M
gO2, 6wt%, Lit 00.6wt%, Al2O
,5+,3 wt%, Na2010°3 wt%, Zn
Glass having a composition of 3 wt% O2 can be suitably used.

By injecting the blood into the acrylic blood collection tube containing the glass fibers mentioned above and centrifuging it, the formed components and fibrinogen in the blood were removed, and the content of fibrinogen and fibrin was reduced to 20 mg/d1. It is possible to collect serum with less variation in content. At this time, it is not necessary to leave the blood still for a long time as in conventional methods, and the blood can be collected and immediately processed by the method of the present invention.

In addition, only fibrinogen and fibrin in the blood can be removed by passing the blood through a tube body such as a glass tube having the above-mentioned glass fibers in the tube, and an anticoagulant can be used. Instead, it is possible to produce samples for whole blood tests.

Furthermore, ascites can be treated by the method of the present invention and then injected into blood vessels for reduction.

(Function) Fibrinogen and fibrin contained in body fluids are removed by bringing body fluids into contact with glass fibers with an average diameter of 9 L or less.

The mechanism is not clear, but it may depend on fibrinoken, which is adsorbed and removed by fibrin or glass fibers.

(Example) Glass fiber 1 with an average diameter of 0.81L obtained by the papermaking method! 52 wt%, glass fiber with an average diameter of 1.5
i 37 wt%, glass fiber with an average diameter of 6JL! 10
Glass paper consisting of 1 wt% PVA binder [thickness 0, 7 mm, 80 g r /m2]
Ventilation resistance 85 mmAq (wind speed 40 cm/
s e c measurement) Pore size average 24L, maximum 27I
Pour 10 ml of freshly collected blood into an acrylic blood collection tube (inner diameter 14 mm) containing a 11.5 mm circular punched tube, and centrifuge the tube at 3,500 rpm for 10 minutes. Serum was separated.

The yield of serum was 2.2 ml (average value of 12 times), and the residual amount of fibrinogen and fibrin in the serum was less than 20 mg/di in all cases, and the variation was extremely small.

In contrast, in the case of the conventional method, the average residual amount of fibrinogen and fibrino in serum was 170 mg/d1, with extremely large variations of 20 to 404 mg/d1.

(Effects of the Invention) Fibrino and fibrinogen in body fluids can be effectively separated and removed without requiring time or manpower.

Claims (1)

[Claims] (1) A method for treating body fluids, which comprises bringing the body fluids into contact with glass fibers having an average diameter of 9 μm or less to remove fibrinogen and fibrin contained in the body fluids.