CN1243251A - Method for separating red blood cells and blood serum and whole blood immunologic function test reagent - Google Patents
Method for separating red blood cells and blood serum and whole blood immunologic function test reagent Download PDFInfo
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- CN1243251A CN1243251A CN 98116438 CN98116438A CN1243251A CN 1243251 A CN1243251 A CN 1243251A CN 98116438 CN98116438 CN 98116438 CN 98116438 A CN98116438 A CN 98116438A CN 1243251 A CN1243251 A CN 1243251A
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Abstract
The present invention relates to a method for separating red blood cell from blood serum in total blood immunodetection reagent, belonging to the blood detection method in the field of disease diagnosis technology. It is characterized by adding a factor capable of resulting in agglutination of red blood cells in the total blood, making the red blood cell agglutinate and producing conglobation reaction, so that the conglobate red bood cells can not be passed through filtering material and membrane with small porosity, and the blood serum can be passed through the membrane so as to attain the goal of separating blood serum from red blood cell to implement quick detection of total blood.
Description
The separation method of red blood cell and serum when the present invention relates to immunologic function test reagent detection whole blood.
When the antigen in the known immunologic function test reagent detection human or animal blood, antibody, need separation of serum and blood cell.Generally the method for Shi Yonging is to use the hydro-extractor centrifuging in advance, and centrifugal back is drawn serum and detected, and the separation of blood cell, serum and the detection of serum can not be carried out on time and space simultaneously, and detected object is actually serum; Another kind of method of separating blood cell and serum is that the polyester material of whole blood by certain pore size filtered, erythrocyte volume is less than or equal to polyester material and is filtered by the latter, serum be able to by, this method can realize blood cell, the separation of serum and detection are carried out simultaneously, detected object is a whole blood, but this method itself has certain volume because of the polyester material, draw a certain amount of whole blood as sponge, the whole blood use amount is big during detection, simultaneously, for guaranteeing separating effect, the polyester aperture is less than and equals the red blood cell diameter, serum and blood cell are less because of pore size filter, velocity of separation is slow, makes the entire reaction time lengthening, and this polyester material has commercial prod, manufacturer is U.S. Ge Erman scientific company (Gelman Sciences Inc.), and its commodity are called HemasepL (the red blood born of the same parents of level parting material) and HemasepV (vertical red blood born of the same parents parting material).
The purpose of this invention is to provide the method for red blood cell and serum in a kind of quick separation trace tip blood, realize that the tachysynthesis of whole blood detects.Aggegation is agglomerating mutually under the aggregation factor effect for red blood cell, and aggegation group amasss in moment and increases thousands of times than single erythrocyte volume.Therefore be easy to be filtered out by fiberglass packing or other large aperture filter material, be easy to realize that in the short time (several seconds) serum separates with erythrocytic, fiberglass packing itself does not adsorb serum, so the whole blood use amount is less, only needs tip blood 100-200ul
The factor that can cause red cell agglutination has two class materials, and a class is a phytolectin, and another kind of is the anti erythrocyte antibody that obtains with the erythrocyte immune animal.Certain density phytolectin and anti erythrocyte antibody mix with whole blood, and it is agglomerating that second more than ten, naked eyes promptly can be observed red cell agglutination, and microscopically is observed, and can be observed thousands of red cell agglutination and is sticked together.Red blood cell and serum separating agent prescription are as follows: one, aqua
(1) damping fluid
0.01M PH 7.2 phosphate buffers (PB)
0.85% NaCL (sodium chloride)
0.2% BSA (bovine serum albumin(BSA))
0.6% trisodium citrate (also can use other anti-coagulants) as heparin, EDTA etc.
5/0,000 NaN
3(Sodium azide)
(2) aggregation factor dilutability
With above-mentioned damping fluid doubling dilution aggregation factor, dilutability 1: 2-1: 1024.Get each dilution separating agent 10ul, add four of whole bloods (about 160ul), the dilutability of red cell agglutination appears in visual inspection 15 seconds or microscopic examination 5 seconds, is the optimal dilution of aggregation factor.
Because of aggregation factor comprises phytolectin One's name is legion, different types of and different types of animal anti erythrocyte antibody, tiring of aggregation factor is also different, uses which kind of dilutability, needs specifically to determine as stated above.
(3) by the optimal dilution of aggregation factor with aggregation factor and damping fluid mix the aqua separating agent.Two, solid-state separating agent
The aqua of Que Dinging is with the saturated back freeze-drying of fiberglass packing or other poriness sorbing material absorption as stated above.
The present invention writes the dilution definite method of prescription, aggregation factor of the damping fluid of understanding red blood cell and serum separating agent, and the preparation method of aqua, solid-state separating agent.
The method that the present invention proposes can be used for the preparation of whole blood tachysynthesis detectable, with existing method ratio, the reagent of preparation can detect the tip whole blood, blood using amount is few, serum, red blood cell velocity of separation are fast, are particularly suitable for the use that family, field, doctor's office, clinic etc. do not have the instrument and equipment place.
The following stated example has described the present invention in detail.1, preparation 0.01M PH7.2 phosphate buffer 1 00ml2, adding NaCL 0.85 gram, trisodium citrate 0.6 gram
NaN
350 milligrams
200 milligrams of bovine serum albumin(BSA)s, mixing 3, with above-mentioned damping fluid doubling dilution goat EA:
1: 2,1: 4,1: 8,1: 16,1: 32,1: 64.........4, respectively get above-mentioned doubling dilution thing 10ul and drip on microslide, add four of finger tip whole bloods (about 160ul) respectively, the jog mixing, observe about 15 seconds, red cell agglutination was all arranged in 1: 2,1: 4,1: 8,1: 16,1: 32, be the antiserum agglutination titer at 1: 32.5, get above-mentioned damping fluid 31ml and add goat anti-human erythrocyte serum 1ml, mixing separating agent.6, separating agent and hepatitis b virus s antigen (HBsAg) immune chromatography test paper are formed kit, hepatitis B HBsAg whole blood immunity chromatography reagent.During use, getting the 10ul separating agent with kapillary or sample injector is added on the immune chromatography test paper reactive end exposure glass fibre, drip four of finger tip blood then on glass fibre, the rapid aggegation of red blood cell is agglomerating and stopped filtration by fiberglass packing and reaction film, the serum that separates can-HBsAg reaction anti-with the gold mark and is moved to the adsorptive pads end along reaction film because of the capillary action of reaction film, 10-20 minute, the serum major part is siphoned away by reaction film by adsorptive pads, serum can be further by reaction film the time be coated on the film anti--HBsAg reacts, and red blood cell is stopped that fully filtration is on fiberglass packing.The result judges: as containing HBsAg in the blood, then form two purplish red lines at reaction surface
As not containing HBsAg in the blood, then form a purplish red line at reaction surface
Claims (1)
- The present invention is the separation method of red blood cell and serum in the whole blood immunity detectable, it is characterized in that in whole blood, sneaking into the red cell agglutination factor as anti--erythrocyte antibody (EA), phytolectin etc., make thousands of red cell agglutination become the visible or observable agglomerate of microscope (anti--erythrocyte antibody (EA) and plant aggegation can be used separately, also can unite use) of naked eyes.Red blood cell is agglomerating because of aggegation, can not be by filtering material and small-bore film, and serum can be by filtering material and small-bore film, thereby reaches separating of blood cell and serum in immunochromatographiassay assay reagent and the immunity percolation detectable testing process, realizes that whole blood tachysynthesis detects.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 98116438 CN1243251A (en) | 1998-07-28 | 1998-07-28 | Method for separating red blood cells and blood serum and whole blood immunologic function test reagent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 98116438 CN1243251A (en) | 1998-07-28 | 1998-07-28 | Method for separating red blood cells and blood serum and whole blood immunologic function test reagent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1243251A true CN1243251A (en) | 2000-02-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 98116438 Pending CN1243251A (en) | 1998-07-28 | 1998-07-28 | Method for separating red blood cells and blood serum and whole blood immunologic function test reagent |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1317394C (en) * | 2000-06-12 | 2007-05-23 | 希森美康株式会社 | Immunoassay and immunoassay apparatus |
| WO2010043113A1 (en) * | 2008-10-13 | 2010-04-22 | Inverness Medical Switzerland Gmbh | Method for separating red blood cells from blood samples and use thereof |
| CN101750244A (en) * | 2008-10-13 | 2010-06-23 | 艾博生物医药(杭州)有限公司 | Method for separating red cells from blood sample and application |
| CN103323308A (en) * | 2013-05-23 | 2013-09-25 | 北京博晖创新光电技术股份有限公司 | Reagent for processing trace whole blood and application thereof |
| CN107796809A (en) * | 2017-10-31 | 2018-03-13 | 三诺生物传感股份有限公司 | A kind of method and its kit and device for the detection of finger tip blood wet method |
| CN108254241A (en) * | 2018-02-06 | 2018-07-06 | 三诺生物传感股份有限公司 | A kind of glycosylated albumin blood sample treatment fluid and its application |
| CN109884042A (en) * | 2019-03-08 | 2019-06-14 | 武汉璟泓万方堂医药科技股份有限公司 | A kind of test paper and its preparation method and application for measuring high-density lipoprotein cholesterol |
| CN110073211A (en) * | 2016-10-05 | 2019-07-30 | 电化生研株式会社 | The agglutination method and separation method and erythrocyte agglutination reagent of red blood cell |
| WO2020220157A1 (en) * | 2019-04-27 | 2020-11-05 | 南京岚煜生物科技有限公司 | Whole blood filtering method and filter membrane structure for whole blood filtering |
| CN113533736A (en) * | 2021-07-15 | 2021-10-22 | 上海伯杰医疗科技有限公司北京分公司 | Mycoplasma pneumoniae IgM antibody colloidal gold immunochromatographic assay detection kit and preparation method thereof |
-
1998
- 1998-07-28 CN CN 98116438 patent/CN1243251A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1317394C (en) * | 2000-06-12 | 2007-05-23 | 希森美康株式会社 | Immunoassay and immunoassay apparatus |
| WO2010043113A1 (en) * | 2008-10-13 | 2010-04-22 | Inverness Medical Switzerland Gmbh | Method for separating red blood cells from blood samples and use thereof |
| CN101750244A (en) * | 2008-10-13 | 2010-06-23 | 艾博生物医药(杭州)有限公司 | Method for separating red cells from blood sample and application |
| CN103323308A (en) * | 2013-05-23 | 2013-09-25 | 北京博晖创新光电技术股份有限公司 | Reagent for processing trace whole blood and application thereof |
| CN103323308B (en) * | 2013-05-23 | 2016-01-06 | 北京博晖创新光电技术股份有限公司 | A kind of reagent and application thereof processing micro whole blood |
| CN110073211A (en) * | 2016-10-05 | 2019-07-30 | 电化生研株式会社 | The agglutination method and separation method and erythrocyte agglutination reagent of red blood cell |
| CN110073211B (en) * | 2016-10-05 | 2021-06-08 | 电化株式会社 | Method for agglutination and separation of red blood cells and reagent for agglutination of red blood cells |
| CN107796809A (en) * | 2017-10-31 | 2018-03-13 | 三诺生物传感股份有限公司 | A kind of method and its kit and device for the detection of finger tip blood wet method |
| CN108254241A (en) * | 2018-02-06 | 2018-07-06 | 三诺生物传感股份有限公司 | A kind of glycosylated albumin blood sample treatment fluid and its application |
| CN109884042A (en) * | 2019-03-08 | 2019-06-14 | 武汉璟泓万方堂医药科技股份有限公司 | A kind of test paper and its preparation method and application for measuring high-density lipoprotein cholesterol |
| WO2020220157A1 (en) * | 2019-04-27 | 2020-11-05 | 南京岚煜生物科技有限公司 | Whole blood filtering method and filter membrane structure for whole blood filtering |
| CN113533736A (en) * | 2021-07-15 | 2021-10-22 | 上海伯杰医疗科技有限公司北京分公司 | Mycoplasma pneumoniae IgM antibody colloidal gold immunochromatographic assay detection kit and preparation method thereof |
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