JPH0477735B2 - - Google Patents

Description

[Detailed description of the invention]

The present invention relates to a halitosis testing method that can easily detect the degree of halitosis. Bad breath is a bad odor that comes out of the oral cavity with exhaled breath, and regardless of its strength, it is unpleasant to other people.
A person's exhaled breath has at least some odor, and the degree of odor varies greatly, but if the degree of odor is low, it is not a big problem. However, there are many people who are overly conscious of their own bad breath, which is insignificant.
In extreme cases, some people develop a type of neurosis called halitosis, in which they avoid others because of their bad breath or are unnecessarily concerned about the way others look at them. In any case, bad breath causes discomfort to others. However, although people can distinguish other people's bad breath as bad, their own bad breath acts as a background, so even if there is a bad odor, it is difficult to perceive it as bad. Even if a person feels uncomfortable, in many cases he or she does not feel any discomfort at all. Therefore,
Knowing the degree of bad breath in your body is useful from an etiquette standpoint, and bad breath can also be caused by diseases such as periodontitis and gingivitis, or changes in your physical condition. It is also useful for discovering diseases and changes in physical condition. The above-mentioned halitosis is caused by halitosis-causing bacteria present in the oral cavity, which metabolize organic matter in the oral cavity such as food residue, exfoliated epithelial cells, white blood cells, and proteins, lipids, and sugars in exudate, resulting in volatile sulfides, lower fatty acids, and amines. , indole, ammonia, and other malodorous components, which are the main cause of malodors. In order to detect bad breath that contains such malodorous components, conventional methods include analyzing exhaled breath using gas chromatography,
A method such as a sensory test of exhaled breath by a person has been adopted. However, the former method, which uses gas chromatography, requires special large-scale equipment and specialized techniques such as collecting exhaled breath using special operations and concentrating it with liquid oxygen or liquid argon. This testing method is very large-scale and expensive, so it is not very practical, and it is difficult to detect cases where bad breath is weak. In addition, the latter method based on a person's senses is difficult to judge one's own bad breath as described above, so the only option is to have someone else test it, which is also not a practical method. Therefore, there is a need for a method that allows people to easily and inexpensively detect their own bad breath. In view of the above circumstances, the present inventors conducted various studies on a simple test method for bad breath, and as a result, they brought saliva into contact with a permanganate such as potassium permanganate, and the The present invention was based on the discovery that the degree of bad breath can be easily and reliably measured by examining the degree of discoloration, and that it is possible to visually detect one's own bad breath easily and inexpensively. It has been reached. The present invention will be explained in more detail below. The halitosis testing method according to the present invention detects bad breath by bringing saliva into contact with permanganate and examining the degree of color change of permanganate at that time.
It is possible to test for the presence or absence of bad breath or the degree of bad breath. Here, the type of permanganate used in the present invention is not particularly limited, but potassium permanganate is preferably used. The method of bringing saliva into contact with permanganate is not particularly limited; for example, a method of preparing a test solution for bad breath testing in which permanganate is dissolved and bringing saliva into contact with this, or a method of contacting saliva with permanganate Preferably, a method is employed in which a sample for testing bad breath is prepared, and saliva is brought into contact with the sample. Here, the above-mentioned test liquid for bad breath test can be obtained by dissolving permanganate in water etc. at an appropriate concentration, but in particular, permanganate of 0.0001
~1% (wt%, same below), more preferably 0.001
Preferably, it is a ~0.1% aqueous solution. In this case, 0.01 to 5% of a surfactant can be added to the test solution for testing bad breath, thereby preventing color comparison from being interfered with by insoluble components in saliva. Note that the addition of a surfactant does not affect the discoloration of permanganate. Further, the surfactant may be any of anionic surfactants, cationic surfactants, amphoteric surfactants, nonionic surfactants, natural surfactants, etc., and one or more of these may be added. I can do it. In addition, the surfactant which can be used is illustrated below. (1) Anionic surfactants a. Fatty acid soaps, sodium laurate, sodium myristate, sodium oleate, sodium stearate, etc. b. Higher alcohol sulfate salts, sodium lauryl sulfate, sodium octyl sulfate, sodium oleyl sulfate, etc. c Secondary higher alcohol sulfate ester salt Arosin type, Tergitol type, Teepol type, heptadecane-2-sulfate, heptadecane-8-sulfate, etc. d Sulfated oil e Sulfuric acid ester salt of higher fatty acid ester f Sulfuric acid of higher fatty acid alkylolamide Ester salt g Nonionic ether sulfate ester salt h Higher alkyl sulfonate i Alkylaryl sulfonate alkylbenzenesulfonate, alkylnaphthalene sulfonate, etc. j Alkylated sulfonate of higher fatty acid amide k Higher fatty acid ester Sulfonate i Dialkyl sulfosuccinate m Higher alkyl phosphate ester (2) Cationic surfactant a Primary amine salt Amine hydrochloride, acetate, e.g. Armac
b Straight chain mono(di)alkyl tri(di)metal such as quaternary amine salt Arguad, Cetavlon, etc.
Linear mono(di)alkyl-tri(di)methyl, etc. with benzyl groups such as Zephirol, Jriton K400 c. Pyridonium-based Ceepryn, IsothanA14, etc. d. Items with amide bonds. Amines with amide bonds such as Separuine, amide bonds such as Zelan. e) Those with ester bonds, such as Soromine, Emulphon FM, etc. f) Those with ether bonds, such as Hyamine1662.10X, Velan, etc. g: Cyclic nitrogen-based imidazole derivatives, Miranol OH, etc. (3) Ampholytic surfactants, alkylglycine type, betaine type , imidazoline type, polyglycolamine sulfate, alkylamine sulfonic acid, etc. (4) Nonionic surfactant a Polyoxyethylene alkyl ether The alkyl group is lauryl, cetyl, oleyl, hydroabiethyl, tridodecyl alcohol, etc., and ethylene Those with an oxide addition mole number of 10 to 100, etc. b Polyoxyethylene alkyl phenol ether alkyl phenol with an alkyl group having carbon numbers of C ₁₀ to C ₂₀ , etc. c Polyoxyethylene alkyl phenol formalin condensate Triton WR-1339 etc. d Polyoxyethylene fatty acid ester e Polyoxyethylene alkyl amide f Alkylol (alkanol) amide g Polyoxyethylene alkylamine h Alkyl mercaptan ether i Polyoxyethylene lanolin alcohol j Higher fatty acid glycerin ester lauroyl monoglyceride, stearyl monoglyceride, etc. k Higher fatty acid Sorbitan esters sorbitan monostearate, sorbitan trilaurate, sorbitan dimyristate, etc. i Sucrose fatty acid esters sucrose monopalmitate, sucrose monostearate, sucrose dilaurate, etc. m Polyoxyethylene sorbitan fatty acid ester n Polyoxyethylene glycerol mono Fatty acid ester o Polyoxyethylene propylene glycol fatty acid ester p Polyoxyethylene sorbitol fatty acid ester q Polyoxyethylene oxyfatty acid triglyceride derivative POE hydrogenated castor oil derivative, etc. r Polyoxyethylene lanolin derivative Lanogel, Selan, etc. s Block polymer type Pluronic, temeronic, alkyl (5) Natural surfactants such as lecithin In addition, a test specimen for halitosis testing in which permanganate is supported on a substrate contains permanganate on a substrate formed into an appropriate size and shape. It can be obtained by impregnating a substrate with a solution containing permanganate, or by applying a permanganate-containing solution to a substrate, and then drying the substrate. In this case, it is preferable to support the permanganate so that its weight when dried is 0.0001 to 1% of the weight of the substrate. The material of the above-mentioned substrate is not particularly limited, but it is preferable that it has water absorption properties due to capillary phenomenon, etc. For example, "New Packaging Technology Handbook" (1971, edited by Japan Packaging Technology Association, published by Japan Productivity Center) Pages 202-251 and 447-486
Paper, woven fabric, non-woven fabric, felt, inorganic porous materials such as fired silica, organic sponge materials such as foamed urethane, capillary tubes such as glass tubes and plastic tubes, water-soluble or highly water-swellable materials listed on the page. Pserogel, glue, etc. made of molecular substances, etc. are preferably used. The base body can be formed into a suitable shape such as a film, and if necessary, the base body may be held by a water-insoluble holder. The test liquid or test sample described above is used by contacting it with saliva, but in this case, the degree of bad breath is determined by contacting it with saliva and then using it.
Judgment can be made by how long it takes for the initial permanganate to discolor, and also by comparing the degree of discoloration with a color sample after a predetermined period of time has passed after contact with saliva. You can also do that.
Furthermore, in the case of a test liquid, the degree of discoloration of permanganate can be determined by measuring absorbance, and the degree of bad breath can be determined from this. In addition, as saliva,
In order to eliminate interference with colorimetry due to insoluble components, saliva from which insoluble components have been removed by centrifugation can be used. As explained above, the method for testing bad breath according to the present invention is simple and simple, by bringing saliva into contact with permanganate and detecting the degree of bad breath based on the degree of color change of the permanganate. It is a device that can inexpensively identify the presence or absence of bad breath and its strength, and can also accurately detect bad breath by oneself at any time and any place, which is difficult to detect by sniffing one's own exhaled breath. It is useful for testing bad breath, as well as for detecting diseases such as gingivitis and periodontitis, as well as changes in physical condition, so it is important to understand the extent of your bad breath on a daily basis. This is an extremely useful method for people who wish to do so or who have oral diseases. Next, examples will be shown to specifically explain the present invention. [Example] The effectiveness of the halitosis testing method of the present invention was investigated by the following method. Test method: Before brushing their teeth, 20 subjects inflated a Flex Sampler odor bag (capacity 3, manufactured by Omi Odoair Service Co., Ltd.) with their breath while resting, and the odor of the breath inside the bag was sensory evaluated by 4 fixed panelists. I let it happen. In addition, the saliva of 20 subjects was collected before tooth brushing, 5 minutes after tooth brushing, and 60 minutes after tooth brushing, and after diluting each of these three times, 3 ml of this diluted solution was added.
0.1M phosphate buffer (PH7.0) 0.5ml and 0.1%
Add 0.2ml of KMnO ₄ aqueous solution and stir, and after 5 minutes
Absorbance was measured at 525 nm. The figure shows the relationship between the sensory evaluation of the breath odor of the subjects (total value of four subjects) using the fixed sampler described above and the absorbance measured using saliva before tooth brushing. The evaluation criteria for the sensory evaluation are as follows. Evaluation criteria There is a very unpleasant odor 3 There is an unpleasant odor 2 There is a faint unpleasant odor 1 There is no unpleasant odor 0 Here, the evaluation is based on the four points from 0 to 3 above in a straight line. The evaluation was performed continuously between 0 and 3 by having the evaluator mark any point on the straight line. Table 1 also shows the absorbance (average value) of potassium permanganate when it was brought into contact with saliva before tooth brushing, 5 minutes after tooth brushing, and 60 minutes after tooth brushing. In addition,
For comparison, the results of a similar experiment using water are also shown in Table 1.

[Table] From the results shown in the drawing, a negative correlation was observed between the evaluation of the subject's exhaled odor by fixed panelists (objective evaluation of bad breath) and the absorbance. That is, the saliva of a person with stronger halitosis lowers the absorbance of potassium permanganate (changes the color more greatly), and it was confirmed that the halitosis testing method of the present invention is effective in detecting halitosis. The results in Table 1 show that saliva before and 60 minutes after toothbrushing, which contains many substances that cause bad breath, greatly lowers the absorbance compared to the control, but toothpaste that does not contain many substances that cause bad breath. 5
It was observed that the absorbance of saliva after 1 minute was not significantly reduced, and it was confirmed that the halitosis testing method of the present invention is effective in detecting halitosis.

[Brief explanation of the drawing]

The drawing is a graph showing the relationship between the sensory evaluation of the breath odor of each subject by a fixed panel and the absorbance of potassium permanganate when the saliva of each subject was brought into contact with the subject before brushing their teeth.

Claims (1)

[Scope of Claims] 1. A method for testing bad breath, which comprises bringing saliva into contact with permanganate and detecting the degree of bad breath based on the degree of color change of the permanganate. 2. The testing method according to claim 1, wherein the permanganate is potassium permanganate. 3. Claim 1, in which saliva is brought into contact with a test solution prepared by dissolving permanganate in water.
Inspection method described in Section 2 or Section 2. 4 The concentration of permanganate in the test solution is 0.0001~
The testing method according to claim 3, wherein the amount is 1% by weight. 5. The testing method according to claim 3 or 4, wherein 0.01 to 5% by weight of a surfactant is added to the test liquid. 6 Claim 1 in which saliva is brought into contact with a test specimen in which permanganate is supported on a substrate.
Inspection method described in section. 7. The testing method according to claim 6, wherein the substrate has water absorption properties. 8. The testing method according to claim 7, wherein the substrate has water absorbency due to capillary action. 9. The testing method according to claim 8, wherein the substrate is selected from paper, woven fabric, nonwoven fabric, and felt. 10. The inspection method according to claim 8, wherein the substrate is an inorganic porous body. 11. The inspection method according to claim 8, wherein the substrate is an organic sponge body. 12. The inspection method according to claim 8, wherein the substrate is a capillary tube. 13. The testing method according to claim 6 or 7, wherein the substrate is pserogel. 14. The testing method according to claim 13, wherein the pserogel is made of a hydrophilic or water-soluble polymeric substance. 15 Claims 6, 7, 8, and 9, in which the substrate is formed in the form of a film.
10, 11, 13, or 14. 16. The testing method according to any one of claims 6 to 15, wherein the substrate is held by a water-insoluble holder.