JPS62294943A - Ozostomia inspection - Google Patents
Ozostomia inspectionInfo
- Publication number
- JPS62294943A JPS62294943A JP61137947A JP13794786A JPS62294943A JP S62294943 A JPS62294943 A JP S62294943A JP 61137947 A JP61137947 A JP 61137947A JP 13794786 A JP13794786 A JP 13794786A JP S62294943 A JPS62294943 A JP S62294943A
- Authority
- JP
- Japan
- Prior art keywords
- degree
- bad breath
- color
- saliva
- reagent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000007689 inspection Methods 0.000 title 1
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 36
- 206010006326 Breath odour Diseases 0.000 claims description 57
- 238000004040 coloring Methods 0.000 claims description 35
- 238000000034 method Methods 0.000 claims description 29
- 238000012360 testing method Methods 0.000 claims description 28
- 210000003296 saliva Anatomy 0.000 claims description 27
- -1 nitrite ions Chemical class 0.000 claims description 23
- 239000006103 coloring component Substances 0.000 claims description 9
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 claims description 8
- 229940005654 nitrite ion Drugs 0.000 claims description 8
- 229910002651 NO3 Inorganic materials 0.000 claims description 6
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims description 3
- 238000011161 development Methods 0.000 claims description 3
- 239000000126 substance Substances 0.000 abstract description 9
- 230000000007 visual effect Effects 0.000 abstract description 7
- 150000002500 ions Chemical class 0.000 abstract description 6
- GQPLMRYTRLFLPF-UHFFFAOYSA-N nitrous oxide Inorganic materials [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 abstract 5
- 230000015572 biosynthetic process Effects 0.000 abstract 2
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 208000032139 Halitosis Diseases 0.000 description 9
- 238000011156 evaluation Methods 0.000 description 7
- 230000035945 sensitivity Effects 0.000 description 7
- 239000000758 substrate Substances 0.000 description 7
- RUFPHBVGCFYCNW-UHFFFAOYSA-N 1-naphthylamine Chemical compound C1=CC=C2C(N)=CC=CC2=C1 RUFPHBVGCFYCNW-UHFFFAOYSA-N 0.000 description 6
- HVBSAKJJOYLTQU-UHFFFAOYSA-N 4-aminobenzenesulfonic acid Chemical compound NC1=CC=C(S(O)(=O)=O)C=C1 HVBSAKJJOYLTQU-UHFFFAOYSA-N 0.000 description 6
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- 230000001680 brushing effect Effects 0.000 description 6
- 239000007864 aqueous solution Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 238000002835 absorbance Methods 0.000 description 4
- 238000002845 discoloration Methods 0.000 description 4
- 239000002253 acid Substances 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 230000001953 sensory effect Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 229950000244 sulfanilic acid Drugs 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- LSDPWZHWYPCBBB-UHFFFAOYSA-N Methanethiol Chemical compound SC LSDPWZHWYPCBBB-UHFFFAOYSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- WZJYKHNJTSNBHV-UHFFFAOYSA-N benzo[h]quinoline Chemical compound C1=CN=C2C3=CC=CC=C3C=CC2=C1 WZJYKHNJTSNBHV-UHFFFAOYSA-N 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000012536 packaging technology Methods 0.000 description 2
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 2
- FDDDEECHVMSUSB-UHFFFAOYSA-N sulfanilamide Chemical compound NC1=CC=C(S(N)(=O)=O)C=C1 FDDDEECHVMSUSB-UHFFFAOYSA-N 0.000 description 2
- 229940124530 sulfonamide Drugs 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 125000003396 thiol group Chemical group [H]S* 0.000 description 2
- APWZAIZNWQFZBK-UHFFFAOYSA-N 1-ethoxynaphthalene Chemical compound C1=CC=C2C(OCC)=CC=CC2=C1 APWZAIZNWQFZBK-UHFFFAOYSA-N 0.000 description 1
- YSEVGCXYIMMVIS-UHFFFAOYSA-N 2-(n-nitrosoanilino)acetic acid Chemical compound OC(=O)CN(N=O)C1=CC=CC=C1 YSEVGCXYIMMVIS-UHFFFAOYSA-N 0.000 description 1
- KIUMMUBSPKGMOY-UHFFFAOYSA-N 3,3'-Dithiobis(6-nitrobenzoic acid) Chemical compound C1=C([N+]([O-])=O)C(C(=O)O)=CC(SSC=2C=C(C(=CC=2)[N+]([O-])=O)C(O)=O)=C1 KIUMMUBSPKGMOY-UHFFFAOYSA-N 0.000 description 1
- TYMLOMAKGOJONV-UHFFFAOYSA-N 4-nitroaniline Chemical compound NC1=CC=C([N+]([O-])=O)C=C1 TYMLOMAKGOJONV-UHFFFAOYSA-N 0.000 description 1
- JOYRKODLDBILNP-UHFFFAOYSA-N Ethyl urethane Chemical compound CCOC(N)=O JOYRKODLDBILNP-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- NULAJYZBOLVQPQ-UHFFFAOYSA-N N-(1-naphthyl)ethylenediamine Chemical compound C1=CC=C2C(NCCN)=CC=CC2=C1 NULAJYZBOLVQPQ-UHFFFAOYSA-N 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-N Nitrous acid Chemical compound ON=O IOVCWXUNBOPUCH-UHFFFAOYSA-N 0.000 description 1
- 208000025157 Oral disease Diseases 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical class [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 1
- 229910021626 Tin(II) chloride Inorganic materials 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229910052788 barium Inorganic materials 0.000 description 1
- DSAJWYNOEDNPEQ-UHFFFAOYSA-N barium atom Chemical compound [Ba] DSAJWYNOEDNPEQ-UHFFFAOYSA-N 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 208000007565 gingivitis Diseases 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229940046892 lead acetate Drugs 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229910052744 lithium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 208000030194 mouth disease Diseases 0.000 description 1
- 150000002823 nitrates Chemical class 0.000 description 1
- 239000004745 nonwoven fabric Substances 0.000 description 1
- 239000000123 paper Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 201000001245 periodontitis Diseases 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000004317 sodium nitrate Substances 0.000 description 1
- 235000010344 sodium nitrate Nutrition 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 229910052712 strontium Inorganic materials 0.000 description 1
- CIOAGBVUUVVLOB-UHFFFAOYSA-N strontium atom Chemical compound [Sr] CIOAGBVUUVVLOB-UHFFFAOYSA-N 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 150000003464 sulfur compounds Chemical class 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 239000002759 woven fabric Substances 0.000 description 1
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Description
【発明の詳細な説明】
３、発明の詳細な説明
１１上外五皿分更
本発明は、自己認知することが困難な口臭の度合を簡便
かつ確実に検知することができる口臭検査方法に関する
。Detailed Description of the Invention 3. Detailed Description of the Invention 11 Top and Outside Five Dishes The present invention relates to a halitosis testing method that can simply and reliably detect the degree of bad breath that is difficult to self-identify.
又米亘皮他
従来より、口臭の度合を判定する方法として、下記■〜
■に示す方法が提案されている。即ち、■ペプトン培地
に唾液を加え、３７℃において１日培養した後、揮発性
硫黄化合物を酢酸鉛等の変色によって検出する方法（特
開昭５６−２１５９８号公報）。Traditionally, methods for determining the degree of bad breath include the following:
The method shown in (2) has been proposed. That is, (1) a method in which saliva is added to a peptone medium and cultured at 37° C. for one day, and then volatile sulfur compounds are detected by discoloration of lead acetate (Japanese Patent Application Laid-open No. 56-21598).
■唾液中のメルカプト基濃度を発色試薬であるビスジメ
チルアミノジフェニル力ルビトールを用いて測定する方
法（特開昭５７−１３５３６０号公報）。(2) A method for measuring the concentration of mercapto groups in saliva using bisdimethylaminodiphenylrubitol, which is a coloring reagent (Japanese Patent Laid-Open No. 135360/1983).
■ジチオビスニトロ安息香酸を用いる方法（特開昭５７
−１４８２５２号公報）。■Method using dithiobisnitrobenzoic acid (Japanese Unexamined Patent Publication No. 57/1989)
-148252).
■呼気中のメチルメルカプタンをメルカプト基発色試薬
（Ｎ Ａ Ｍ ）と反応させ、螢光によって口臭を測定
する方法（特開昭５８−１９１９５７号公報）。(2) A method of reacting methyl mercaptan in exhaled breath with a mercapto group coloring reagent (NAM) and measuring bad breath using fluorescence (Japanese Patent Laid-Open No. 191957/1983).
■呼気中のガス成分のガス検知素子への吸着による抵抗
値の変化を電気信号に変換して測定することにより１口
臭を検知する方法（特開昭５８−２０８６５１号公報）
。■Method of detecting bad breath by converting and measuring the change in resistance value due to the adsorption of gas components in exhaled breath to a gas detection element into an electrical signal (Japanese Patent Laid-Open No. 58-208651)
.
■含硫アミノ酸と唾液との反応によって発生した臭気強
度から口臭を検知する方法（特開昭６０−４８５９号公
報）。(2) A method for detecting bad breath from the odor intensity generated by the reaction between sulfur-containing amino acids and saliva (Japanese Patent Application Laid-Open No. 60-4859).
■過マンガン酸塩に唾液を接触させ、その際の過マンガ
ン酸塩の変色度によって口臭を検知する方法（特開昭６
０−１７８８２８号公報）。■A method of bringing saliva into contact with permanganate and detecting bad breath based on the degree of discoloration of the permanganate (Japanese Patent Laid-Open No. 6
0-178828).
日が しようとするル 占
しかしながら、上記■〜■の方法は、それぞれ以下に示
す問題点を有する。即ち、
■の方法：測定に２４時間という長時間を要し。However, each of the above methods (■ to ■) has the following problems. That is, method (2): Measurement takes a long time of 24 hours.
口臭を迅速に検査できない。Bad breath cannot be tested quickly.
■〜■の方法：測定感度が低く、口臭を確実に検知する
ことが困難である。Methods (1) to (2): The measurement sensitivity is low, making it difficult to reliably detect bad breath.
■の方法：検知素子にガス成分に対する特異性がなく、
香料成分等に対しても感じてしまうため、口臭のみを正
確に測定することができない。Method ■: The detection element has no specificity for gas components,
It is not possible to accurately measure only bad breath because the smell is also felt by fragrance ingredients.
■の方法：検査結果を視覚的に認識することができない
ため、検査方法としての魅力に欠ける。Method (2): This method is unattractive as a testing method because the test results cannot be visually recognized.
■の方法：検査を短時間で行なうことができ。■Method: Tests can be carried out in a short time.
かつ検査結果を視覚的に認識することができ１口臭検査
方法として比較的簡便に採用し得、有効な方法であるが
、なお、試薬が不安定であり、しかも着色の安定性が必
ずしも良くないという問題がある。In addition, the test results can be visually recognized, and it is relatively easy to use and effective as a halitosis test method, but the reagent is unstable and the stability of the coloring is not necessarily good. There is a problem.
このように、従来の口臭測定方法は種々の欠陥を有する
ものであり、このため従来より口臭を迅速、簡便、かつ
正確に検査し得ると共に、検査結果を視覚によって容易
にしかも安定して認識することのできる口臭検査方法が
望まれている。As described above, conventional methods for measuring bad breath have various deficiencies, and therefore, it is possible to test for bad breath more quickly, easily, and accurately than before, and to recognize test results easily and stably visually. A method for testing bad breath that can be used is desired.
口 占 するための び
本発明者らは、上記事情に鑑み、迅速、簡便かつ正確に
、しかも視覚によって口臭の度合を安定して判定するこ
とのできる口臭検査方法を提案するため、唾液中に存在
する口臭の指標となる物質を探索し、これらの物質から
口臭程度を検査することについて種々検討を行なった結
果、メルカプト系化合物を指標として直接唾液から口臭
を検知することは感度的に無理があること、これに対し
唾液中に存在する亜硝酸イオンの量が口臭の程度に良好
に対応し、亜硝酸イオンを指標とすることにより、直接
唾液から口臭を測定し得ることを見い出した。そして亜
硝酸イオンと反応して発色する発色成分を含む発色試薬
によって唾液中の亜硝酸イオンを検出することにより、
極めて高感度で口臭の度合を検知し得ることを知見し１
本発明をなすに至ったものである。In view of the above circumstances, the inventors of the present invention have proposed a halitosis test method that can quickly, easily, accurately, and visually visually determine the degree of bad breath. After conducting various studies to search for substances that are indicators of bad breath and to test the degree of bad breath using these substances, we found that it is impossible to directly detect bad breath from saliva using mercapto compounds as an indicator due to sensitivity. In contrast, we have found that the amount of nitrite ions present in saliva corresponds well to the degree of bad breath, and that by using nitrite ions as an indicator, it is possible to directly measure bad breath from saliva. Then, by detecting nitrite ions in saliva with a coloring reagent containing a coloring component that develops color by reacting with nitrite ions,
We discovered that it is possible to detect the degree of bad breath with extremely high sensitivity.1
This is what led to the present invention.
従って、本発明は、亜硝酸イオンと反応して発色する発
色成分を含む亜硝酸イオン発色試薬に唾液を接触させ、
その際の亜硝酸イオン発色試薬の発色程度によって口臭
の度合を検知するようにしたことを特徴とする口臭検査
方法を提供することを目的とする。Therefore, the present invention involves bringing saliva into contact with a nitrite ion coloring reagent containing a coloring component that develops color by reacting with nitrite ions,
It is an object of the present invention to provide a method for testing bad breath, which is characterized in that the degree of bad breath is detected based on the degree of coloring of a nitrite ion coloring reagent.
本発明においては、上記発色試薬に唾液を接触させるよ
うにしたことにより１発色試薬中の亜硝酸、イオンと反
応して発色する発色成分と唾液中の口臭指標物質である
亜硝酸イオンとが反応し、亜硝酸イオンの址に対応した
強さで発色試薬が発色する。従って、発色試薬の発色程
度を調べることにより、口臭の度合を検知できるもので
ある。この場合、口臭指標物質として唾液中の亜硝酸イ
オンを選択し、この亜硝酸イオンによって発色試薬を発
色させるという方法を採用したことにより、唾液中の亜
硝酸イオン量に応じて発色試薬が極めて高感度で発色し
、かつ着色の安定性が高く、従って本発明によれば正確
に、かつ高感度で口臭を測定できる。即ち、上述したよ
うに、メルカプト系化合物を指標物質とする検査方法は
感度及び着色の安定性に問題を有していたが、本発明に
おいては亜硝酸イオンを指標物質としたことにより。In the present invention, by bringing saliva into contact with the coloring reagent, the coloring component that reacts with nitrous acid and ions in the coloring reagent to form a color reacts with nitrite ions, which are bad breath indicator substances in saliva. Then, the coloring reagent develops a color with an intensity corresponding to the amount of nitrite ion. Therefore, the degree of bad breath can be detected by examining the degree of color development of the color reagent. In this case, by selecting nitrite ions in saliva as the bad breath indicator and using the nitrite ions to color the coloring reagent, the coloring reagent becomes extremely high depending on the amount of nitrite ions in saliva. It develops color with high sensitivity and has high coloring stability. Therefore, according to the present invention, bad breath can be measured accurately and with high sensitivity. That is, as mentioned above, testing methods using mercapto compounds as indicator substances had problems with sensitivity and coloring stability, but in the present invention, nitrite ions were used as indicator substances.
このようなメルカプト系化合物を指標とする場合の欠陥
を解消したものである。This method overcomes the drawbacks of using such mercapto compounds as indicators.
以下、本発明につき更に詳しく説明する。The present invention will be explained in more detail below.
本発明に係る口臭検査方法は、亜硝酸イオンと反応して
発色する発色成分を含む亜硝酸イオン発色試薬に唾液を
接触させ、その際の発色試薬の発色程度を調べることに
より口臭を検知するもので、口臭の有無或いは口臭の程
度を検査することができるものである。The halitosis testing method according to the present invention detects bad breath by bringing saliva into contact with a nitrite ion coloring reagent containing a coloring component that develops color by reacting with nitrite ions, and examining the degree of color development of the coloring reagent at that time. It is possible to test for the presence or absence of bad breath or the degree of bad breath.
ここで、本発明において用いる亜硝酸イオン発色試薬の
種類は特に制限されないが、発色成分として例えばスル
ファニルアミド、α−ナフチルエチレンジアミン、スル
ファニル酸、α−ナフチルアミン、スルファミン、３−
ヒドロキシ−１，２，３，４−テトラヒドロ−７，８−
ベンゾキノリン、Ｐ−ニトロアニリン、フェノール、エ
チル−１−ナフチルエーテル、Ｎ−ニトロソフェニルグ
リシン、Ｎ−メチル−Ｎ−ニトロソ−３，６−シニトロ
アニリン等の１種又は２種以上を使用することができ、
この発色成分を直接発色試薬として使用したり、或いは
発色成分を水若しくは酸等に溶解して発色試薬を調製す
ることができる。なお、このように発色試薬を水若しく
は酸等に溶解して溶液状の発色試薬を調製する場合、そ
の溶液中の発色成分濃度に限定はないが１発色成分濃度
が０．０００１〜１０％（重量％、以下同じ）、特に０
．００１〜１％となるように調製することが好ましい。Here, the type of nitrite ion coloring reagent used in the present invention is not particularly limited, but examples of coloring components include sulfanilamide, α-naphthylethylenediamine, sulfanilic acid, α-naphthylamine, sulfamine, 3-
Hydroxy-1,2,3,4-tetrahydro-7,8-
One or more of benzoquinoline, P-nitroaniline, phenol, ethyl-1-naphthyl ether, N-nitrosophenylglycine, N-methyl-N-nitroso-3,6-sinitroaniline, etc. may be used. is possible,
This color-forming component can be used directly as a color-forming reagent, or a color-forming reagent can be prepared by dissolving the color-forming component in water, acid, or the like. In addition, when preparing a coloring reagent in the form of a solution by dissolving the coloring reagent in water or acid, etc., there is no limit to the concentration of the coloring component in the solution, but the concentration of 1 coloring component is 0.0001 to 10% ( weight%, the same applies hereinafter), especially 0
．． It is preferable to adjust the amount to 0.001 to 1%.
ここで、本発明においては、上記発色試薬に硝酸塩を配
合することができ、これにより口臭をより確実に検知す
ることができる。即ち、メルカプト化合物を産生ずる口
臭原因菌は硝酸イオンを亜硝酸イオンに還元する代謝を
行なうが、このように発色試薬に硝酸塩を配合すること
により１口臭原因菌の量に対応する硝酸イオンの亜硝酸
イオンへの還元量をも含めて口臭の検知を行なうことが
でき、従ってより正確な測定がなされるものである。な
お、硝酸塩としては硝酸のナトリウム、カリウム、スト
ロンチウム、銀、バリウム、マグネシウム、マンガン、
リチウム、スズ塩等が使用できる。Here, in the present invention, a nitrate can be added to the coloring reagent, thereby making it possible to detect bad breath more reliably. In other words, halitosis-causing bacteria that produce mercapto compounds metabolize nitrate ions to reduce them to nitrite ions, and by adding nitrate to the coloring reagent in this way, the amount of nitrate ions corresponding to the amount of halitosis-causing bacteria can be reduced. It is possible to detect bad breath including the amount reduced to nitrate ions, and therefore more accurate measurements can be made. In addition, nitrates include sodium, potassium, strontium, silver, barium, magnesium, manganese, and
Lithium, tin salt, etc. can be used.
また、発色試薬に唾液を接触させる方法は特に制限され
ず１例えば上述した溶液状発色試薬を調製してこれに唾
液を接触させる方法や、基体に発色試薬を担持させた口
臭検査用試験体を調製し、これに唾液を接触させる方法
などが好適に採用される。The method of bringing saliva into contact with the coloring reagent is not particularly limited; for example, the method of preparing the above-mentioned solution coloring reagent and bringing saliva into contact with it, or the method of bringing saliva into contact with the coloring reagent on a substrate, or the method of bringing saliva into contact with the coloring reagent on a substrate. Preferably, a method is employed in which the saliva is prepared and brought into contact with saliva.
ここで、上述した発色試薬を担持させた口臭検査用試験
体は、基体に酸や水に発色成分を溶解した発色試薬を含
浸したり、塗布することなどにより得ることができる。Here, the test body for halitosis testing carrying the above-described coloring reagent can be obtained by impregnating or coating a substrate with a coloring reagent prepared by dissolving a coloring component in acid or water.
また、更にこれを乾燥させてもよい。この場合、発色成
分は乾燥時に基体重量の０．００００１〜１％となるよ
うに担持させることが好ましい。なお、上記基体の材質
は特に制限されないが、毛細管現象等による吸水性を有
するものであることが好ましく、例えば「新包装技術便
覧Ｊ （１９７１年１日本包装技術協会編、日本生産
性本部発行）第２０２〜２５１頁及び第４４７〜４８６
真に記載された紙、織布、不織布、フェルト、焼成され
たシリカ等の無機多孔質体、発泡ウレタン等の有機スポ
ンジ体、ガラス管、プラスチック管等のキャピラリ管、
水溶性若しくは水膨潤性高分子物質等からなる乾厘体、
こう厘体などが好適に用いられる。なお、基体はフィル
ム状等の適宜な形状に形成することができ、更に必要に
より基体を水不溶性の保持体に保持させるようにしても
よい。Moreover, this may be further dried. In this case, it is preferable that the color-forming component be supported in an amount of 0.00001 to 1% of the weight of the substrate upon drying. The material of the above-mentioned substrate is not particularly limited, but it is preferable that it has water absorbency due to capillary phenomenon, etc., for example, "New Packaging Technology Handbook J (edited by Japan Packaging Technology Association, 1971, published by Japan Productivity Center)". Pages 202-251 and 447-486
Truly described paper, woven fabric, non-woven fabric, felt, inorganic porous bodies such as fired silica, organic sponge bodies such as urethane foam, capillary tubes such as glass tubes and plastic tubes,
A dry body made of water-soluble or water-swellable polymeric substances, etc.
A cylindrical body or the like is preferably used. The substrate can be formed into a suitable shape such as a film, and if necessary, the substrate may be held in a water-insoluble holder.
上記した試験液或いは試験体は、上述したように唾液に
接触させて使用するものであるが、この場合口臭の程度
の判定は、例えば唾液に接触させた後、発色試薬の変色
の程度を色見本と比較するなどして調べることによって
行なうことができる。The above test solution or test sample is used by bringing it into contact with saliva, but in this case, the degree of bad breath can be determined by, for example, measuring the degree of discoloration of a color reagent after contact with saliva. This can be done by comparing it with a sample.
更に、試験液の場合は、吸光度測定により発色試薬の変
色度を調べ、これから口臭の度合を判定することもでき
る。Furthermore, in the case of a test liquid, the degree of discoloration of the coloring reagent can be determined by measuring absorbance, and the degree of bad breath can also be determined from this.
２１Ｂ針佐釆−
以上説明したように、本発明の口臭検査方法は簡便かつ
安価に口臭の有無、強弱を識別し得るものであり、しか
も自分の呼気を嗅いだのでは検知することの困難な口臭
を自分自身で任意の時間、任意の場所において正確に検
知することができ、口臭の検査に役立つものであると同
時に、歯肉炎。21B Harisama - As explained above, the halitosis testing method of the present invention can easily and inexpensively identify the presence or absence of bad breath, and its strength. It is possible to accurately detect bad breath yourself at any time and in any location, which is useful for testing bad breath, as well as gingivitis.
歯周炎といった疾患や体調の変化等の発見にも役立つも
のである。それ故、日常の自分の口臭の程度を把握して
おきたいという希望を有する人や口腔疾患を有する人に
とって極めて有用な方法である。この場合、本発明にお
いては口臭指標物質として亜硝酸イオンを選択し、亜硝
酸イオンによる発色反応によって口臭を判定するように
したので。It is also useful for discovering diseases such as periodontitis and changes in physical condition. Therefore, it is an extremely useful method for people who wish to understand the degree of bad breath they have on a daily basis and for people who have oral diseases. In this case, in the present invention, nitrite ions are selected as the bad breath indicator substance, and bad breath is determined by a coloring reaction caused by the nitrite ions.
発色感度が高く、かつ着色も安定しており、従って本発
明によれば口臭の程度を迅速、簡便、かつ正確に、しか
も視覚によって安定して判定し得るものである。The coloring sensitivity is high and the coloring is stable. Therefore, according to the present invention, the degree of bad breath can be determined quickly, easily, accurately, and stably visually.
次に実施例を示し、本発明を具体的に説明するが、本発
明は下記実施例に限定されるものではなし１゜
〔実施例１〕
下記方法により本発明口臭検査方法の有効性を調べた。Next, the present invention will be specifically explained with reference to examples, but the present invention is not limited to the following examples.1゜[Example 1] The effectiveness of the halitosis testing method of the present invention was investigated by the following method. Ta.
盆暮友蒸
歯磨前の２０名の被験者にフレックサンプラー臭突（容
量３Ｑ、近江オドエアサービス社製）を安静下に呼気で
ふくらまさせ、袋内の呼気の臭いを固定パネラ−４名に
官能評価させた。Before Bongo Tomo steamed toothbrushing, 20 subjects were asked to inflate a Flex Sampler Odor Pond (capacity 3Q, manufactured by Omi Odoair Service Co., Ltd.) with their breath while resting, and the odor of the exhaled air inside the bag was fixed on a panel of 4 subjects. I had it evaluated.
また、０．３％スルファニル酸を含有する１０％酢酸水
溶液５０ＩＡ及び１％α−ナフチルアミン水溶液５０μ
党を紙片に含浸させて本発明口臭検査用試験紙を調製す
ると共に、被験者２０名の歯磨前、歯磨５分後及び歯磨
６０分後の唾液をそれぞれ採取し、これら唾液それぞれ
１００バを上記試験紙に付着させ、着色度を視覚判定し
た。In addition, 50 IA of 10% acetic acid aqueous solution containing 0.3% sulfanilic acid and 50μ of 1% α-naphthylamine aqueous solution
A test strip for bad breath testing of the present invention was prepared by impregnating a piece of paper with the substance, and saliva was collected from 20 test subjects before brushing their teeth, 5 minutes after brushing their teeth, and 60 minutes after brushing their teeth, and 100 samples of each of these saliva were used in the above test. It was attached to paper and the degree of coloration was visually judged.
上記の固定サンプラーによる被験者の呼気の臭いの官能
評価（４名の合計値）と試験紙の着色度の視覚判定との
関係を図面に示す。なお、上記官能評価及び視覚判定の
評価基準は下記の通りである。The figure shows the relationship between the sensory evaluation of the breath odor of the subjects (total value of four subjects) using the above-mentioned fixed sampler and the visual judgment of the degree of coloration of the test paper. The evaluation criteria for the above sensory evaluation and visual judgment are as follows.
′も の評価基準
不快な臭いはしない ０
かすかに不快な臭いがする １不快な臭いがす
る ２非常に不快な臭いがする
３′判、の。価基型
発色しない −やや発色する
±
発色する 十強く発色する
＋＋〔実施例２〕
実施例１と同じ被験者２０名の歯磨前、歯磨５分後及び
歯磨６０分後の唾液をそれぞれ採取すると共に、これら
唾液０．１ｍＱに０．１％スルファニル酸を含有する１
０％酢酸水溶液ｌ！Ｊｉ。Evaluation criteria: There is no unpleasant odor 0 There is a faint unpleasant odor 1 There is an unpleasant odor 2 There is a very unpleasant odor
3' size. Value-based type does not develop color - Some color develops
± Color develops Ten strong color develops
++ [Example 2] Saliva was collected from the same 20 subjects as in Example 1 before tooth brushing, 5 minutes after tooth brushing, and 60 minutes after tooth brushing, and 0.1 mQ of these saliva contained 0.1% sulfanilic acid. 1
0% acetic acid aqueous solution l! Ji.
０．５％α−ナフチルアミン水溶液０．８ｄ及び０．０
１％硝酸ナトリウム水溶液０．１ｊ）を加えて攪拌し、
３７°で１０分間インキュベーションを行なった後、５
２５ｎｍにおける吸光度を測定した。この時の発色化合
物の吸光度（平均値）を第１表に示す。なお、比較のた
め水を用いて同様の実験を行なった結果を第１表に併記
する。0.5% α-naphthylamine aqueous solution 0.8d and 0.0
Add 1% sodium nitrate aqueous solution 0.1j) and stir,
After 10 min incubation at 37°,
Absorbance was measured at 25 nm. Table 1 shows the absorbance (average value) of the coloring compound at this time. For comparison, the results of a similar experiment using water are also listed in Table 1.
第 １ 表
図面及び第１表の結果より、固定パネラ−による被験者
の呼気の臭いの評価（他覚による口臭の評価）と本発明
方法による視見判定結果との間、及びこの視覚判定結果
と吸光度（亜硝酸イオンの量に対応する）との間には良
好な相関関係が認められ、従って本発明により口臭の度
合を正確に検知し得ることが確認された。From the drawings in Table 1 and the results in Table 1, it is clear that there is a difference between the evaluation of the breath odor of the subject by a fixed panel (objective evaluation of bad breath) and the visual judgment result by the method of the present invention, and the difference between the visual judgment result and this visual judgment result. A good correlation was observed between the absorbance (corresponding to the amount of nitrite ions) and it was therefore confirmed that the degree of bad breath can be accurately detected by the present invention.
図面は固定パネラ−による各被験者の呼気の臭いの官能
評価と試験紙の着色度を視覚判定した結果との関係を示
すグラフである。The figure is a graph showing the relationship between the sensory evaluation of the breath odor of each subject by a fixed panel and the visual judgment of the degree of coloration of the test paper.
Claims (1)
硝酸イオン発色試薬に唾液を接触させ、その際の亜硝酸
イオン発色試薬の発色程度によって口臭の度合いを検知
するようにしたことを特徴とする口臭検査方法。 ２、亜硝酸イオン発色試薬が硝酸塩を含むものである特
許請求の範囲の範囲第１項記載の口臭検査方法。[Claims] 1. Bringing saliva into contact with a nitrite ion coloring reagent containing a coloring component that develops color by reacting with nitrite ions, and detecting the degree of bad breath based on the degree of color development of the nitrite ion coloring reagent at that time. A method for testing bad breath, which is characterized by: 2. The method for testing bad breath according to claim 1, wherein the nitrite ion coloring reagent contains nitrate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61137947A JPS62294943A (en) | 1986-06-13 | 1986-06-13 | Ozostomia inspection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61137947A JPS62294943A (en) | 1986-06-13 | 1986-06-13 | Ozostomia inspection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62294943A true JPS62294943A (en) | 1987-12-22 |
| JPH0511858B2 JPH0511858B2 (en) | 1993-02-16 |
Family
ID=15210424
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61137947A Granted JPS62294943A (en) | 1986-06-13 | 1986-06-13 | Ozostomia inspection |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62294943A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024075783A1 (en) * | 2022-10-05 | 2024-04-11 | 沢井製薬株式会社 | Method for quantifying reactive nox, and formulation for suppressing generation of nitroso compounds |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58191957A (en) * | 1982-05-04 | 1983-11-09 | Lion Corp | Method for measuring oral odor |
-
1986
- 1986-06-13 JP JP61137947A patent/JPS62294943A/en active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58191957A (en) * | 1982-05-04 | 1983-11-09 | Lion Corp | Method for measuring oral odor |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024075783A1 (en) * | 2022-10-05 | 2024-04-11 | 沢井製薬株式会社 | Method for quantifying reactive nox, and formulation for suppressing generation of nitroso compounds |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0511858B2 (en) | 1993-02-16 |
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