JPH0475735B2 - - Google Patents
Info
- Publication number
- JPH0475735B2 JPH0475735B2 JP1174285A JP17428589A JPH0475735B2 JP H0475735 B2 JPH0475735 B2 JP H0475735B2 JP 1174285 A JP1174285 A JP 1174285A JP 17428589 A JP17428589 A JP 17428589A JP H0475735 B2 JPH0475735 B2 JP H0475735B2
- Authority
- JP
- Japan
- Prior art keywords
- rotifers
- feed
- tank
- culture
- culture tank
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 241000700141 Rotifera Species 0.000 claims description 48
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- 239000013535 sea water Substances 0.000 claims description 16
- 238000003306 harvesting Methods 0.000 claims description 14
- 238000012258 culturing Methods 0.000 claims description 8
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 4
- 241000224474 Nannochloropsis Species 0.000 claims description 3
- 239000013505 freshwater Substances 0.000 claims description 3
- 241001465754 Metazoa Species 0.000 claims description 2
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 claims description 2
- 239000011715 vitamin B12 Substances 0.000 claims description 2
- 229930003779 Vitamin B12 Natural products 0.000 claims 1
- 238000012364 cultivation method Methods 0.000 claims 1
- 238000001471 micro-filtration Methods 0.000 claims 1
- 235000019163 vitamin B12 Nutrition 0.000 claims 1
- 238000000034 method Methods 0.000 description 12
- 238000001914 filtration Methods 0.000 description 6
- 238000012136 culture method Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 241000238424 Crustacea Species 0.000 description 2
- 230000002542 deteriorative effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 239000004575 stone Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000223782 Ciliophora Species 0.000 description 1
- 208000002720 Malnutrition Diseases 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 235000018343 nutrient deficiency Nutrition 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Feed For Specific Animals (AREA)
- Farming Of Fish And Shellfish (AREA)
- Fodder In General (AREA)
Description
【発明の詳細な説明】
〔産業上の利用分野〕
この発明はプランクトンの連続培養法、より詳
細に述べると種苗生産における魚類および甲殻類
等の初期飼料としての動物性プランクトン、とく
にワムシを連続的に培養する方法に関する。[Detailed Description of the Invention] [Field of Industrial Application] This invention relates to a method for continuously cultivating plankton, and more specifically, to continuously culturing zooplankton, especially rotifers, as initial feed for fish and crustaceans in seed production. Regarding the method of culturing.
種苗生産における魚類および甲殻類等の初期飼
料としては、一般に動物性プランクトン、とくに
「シオミズツボワムシ」が使用されている。
In seed production, zooplankton, in particular "Shiomid rotifer", is generally used as initial feed for fish, crustaceans, etc.
とくに、これら飼料を大量に培養するに当つて
は、培養期間、接種量、給餌量などの培養条件を
一定にして、最初の接種量から所定の密度にまで
培養して、原則として中間での間引きを行うこと
なく収獲する植継ぎ法、すなわちバツチ法があ
る。 In particular, when culturing these feeds in large quantities, the culture conditions such as culture period, amount of inoculation, and amount of feeding should be kept constant, and as a general rule, culturing should be carried out from the initial amount of inoculation to a predetermined density. There is a transplanting method that allows harvesting without thinning, namely the batch method.
また、間引き法として、１日または数日おきに
培養液中の全体のワムシの一部を収獲する方法が
ある。 Furthermore, as a thinning method, there is a method in which a portion of all the rotifers in the culture solution are harvested every day or every few days.
従来これらの方法によるワムシの培養におい
て、これを実施している現場からの知見を集約す
ると、その培養の過程において、ワムシの増殖の
不良、あるいは急激な個体数の減少が問題点とし
て挙げられる。その原因としては、(1)ワムシの排
泄物、残餌料などが培養槽に残留して水質環境が
悪化すること、(2)餌料の栄養不足、(3)水温あるい
は塩分等の物理化学的条件の変動、(4)給餌の過不
足などが考えられる。 Conventionally, when culturing rotifers using these methods, the knowledge from the field where this is practiced shows that problems include poor proliferation of rotifers or a rapid decrease in the number of rotifers during the culture process. The causes of this are (1) rotifer excrement and residual feed remaining in the culture tank, deteriorating the water quality environment, (2) nutritional deficiencies in the feed, and (3) physicochemical conditions such as water temperature or salinity. (4) Overfeeding or underfeeding.
以上に述べた従来の動物性プランクトンの培養
法における多くの問題点を考慮して、この発明の
目的は培養液中の水質あるいは環境などを悪化さ
せることなく、安定した収量のワムシを連続的に
得ることのできるワムシの連続培養法を提供する
ことにある。
Considering the many problems in the conventional zooplankton culture methods mentioned above, the purpose of this invention is to continuously grow rotifers with a stable yield without deteriorating the water quality in the culture solution or the environment. The object of the present invention is to provide a continuous culturing method for rotifers that can be obtained.
この発明の目的はまた、槽内の水質環境に限ら
ず、餌料環境をも一定に維持し給餌、収獲を連続
的におこなうワムシの連続培養法を提供すること
にある。 Another object of the present invention is to provide a method for continuously cultivating rotifers in which not only the water quality environment in the tank but also the food environment is maintained constant and feeding and harvesting is performed continuously.
以上の目的を達成するために、この発明はワム
シの培養水槽に所定量の餌料を含む海水を定量的
に供給する一方、培養水槽内のワムシを定量的に
収獲して、培養水槽内のワムシの個体数を常に一
定に保持することにある。
In order to achieve the above objects, the present invention quantitatively supplies seawater containing a predetermined amount of food to a rotifer culture tank, and quantitatively harvests rotifers in the culture tank. The goal is to maintain a constant number of individuals.
次に、この発明のワムシの連続培養法を具体的
に説明する。先ず、この発明の方法を実施するた
めには、第１図に示すように、少くとも海水濾過
装置１０と、餌料槽１２と、培養槽１４と、収獲
槽１６とをそれぞれ餌料供給用の定量ポンプ１８
とワムシ収獲用の定量ポンプ２０を具備する流体
通路２２，２４によつて相互に連結した施設を用
意する。 Next, the rotifer continuous culture method of the present invention will be specifically explained. First, in order to carry out the method of the present invention, as shown in FIG. pump 18
A facility is provided which is interconnected by fluid passages 22, 24 and equipped with a metering pump 20 for harvesting rotifers.
海水濾過装置１０は以上に述べた各槽に海水を
供給する最初の段階の装置である。 The seawater filtration device 10 is a first-stage device that supplies seawater to each tank described above.
海水濾過装置１０は、ワムシを増殖培養する培
養液中に、ワムシと餌が競合するする海水中の他
の微小動物、たとえば繊毛虫、その他ワムシの培
養に支障のあるものが混入することのないように
海水を精密濾過するための装置である。したがつ
て複数段のフイルタ装置などを使用して、専ら不
純物の存在しない海水を餌料槽１２および培養槽
１４へ供給する。 The seawater filtration device 10 prevents the mixing of other microscopic animals in the seawater that compete with rotifers for food, such as ciliates, and other substances that may impede the cultivation of rotifers, into the culture solution in which rotifers are grown and cultured. This is a device for precisely filtering seawater. Therefore, a multi-stage filter device or the like is used to supply exclusively impurity-free seawater to the feed tank 12 and the culture tank 14.
餌料槽１２には海水濾過装置１０を経て供給さ
れた海水にワムシの培養に最適の餌料を混入し、
しかも餌料の混入密度を適正に調整する。 In the food tank 12, the seawater supplied through the seawater filtration device 10 is mixed with the optimal food for culturing rotifers.
Moreover, the mixing density of the feed is appropriately adjusted.
ここで使用する餌料はナンノクロロプシス（一
般に「海産クロレラ」という）、淡水産クロレラ
および酵母などである。ナンノクロロプシスは経
済的に多量のものを供給することが困難であるこ
とから、淡水産クロレラにビタミンB12などを添
加して使用する。 The feeds used here include Nannochloropsis (generally referred to as "marine chlorella"), freshwater chlorella, and yeast. Since it is economically difficult to supply Nannochloropsis in large quantities, freshwater Chlorella is used with vitamin B12 added.
培養槽１４は供給された海水中でワムシを増殖
培養する基本的な水槽である。所定量のワムシを
供給し、これを一様に液中に分布させるために、
撹拌機２６とエアストーン装置２８とを水槽内に
配設し、また水温を所定の温度、たとえば30℃に
維持するために、ヒーター３０及び温度センサ３
２などの装置を設備する。 The culture tank 14 is a basic aquarium in which rotifers are grown and cultured in supplied seawater. In order to supply a predetermined amount of rotifers and distribute them uniformly in the liquid,
A stirrer 26 and an air stone device 28 are arranged in the water tank, and a heater 30 and a temperature sensor 3 are installed in order to maintain the water temperature at a predetermined temperature, for example, 30°C.
Equipment such as 2 will be installed.
培養槽１４内で、ワムシの固体数を一定に維持
するためには、餌料槽１２から餌料供給ポンプ１
８によつて通路２２を経て培養槽１４に送られる
餌料混入濾過水によつてワムシ密度が希釈される
割合と、培養槽１４内でワムシ自身が増殖してそ
の個体数が増加してゆく割合とが釣合わなくては
ならない。このためには、ワムシの比増殖率の算
出と、それに基いた餌料混入濾過水の移送量の調
整を（少くとも）１日１回程度おこなうことが必
要である。あるいはまた、これを１日１回程度お
こなえば、安定した培養を継続することができ
る。 In order to maintain a constant number of rotifers in the culture tank 14, the feed supply pump 1 must be removed from the feed tank 12.
8, the rate at which the rotifer density is diluted by the feed-containing filtered water sent to the culture tank 14 via the passage 22, and the rate at which the rotifers themselves proliferate in the culture tank 14 and their population increases. must be balanced. For this purpose, it is necessary to calculate the specific growth rate of rotifers and adjust the amount of feed-containing filtered water to be transferred based on the calculation (at least) once a day. Alternatively, if this is done about once a day, stable culture can be continued.
従来のワムシの培養法では、培養槽内の水がか
なり長時間入れかわらないために、その水質環境
が悪化の一途をたどり、それがワムシの増殖の度
合を変化させる原因になつていたのである。 In conventional rotifer culture methods, the water in the culture tank was not replaced for a considerable period of time, which caused the water quality to deteriorate, which caused changes in the rate of rotifer proliferation. .
これに反して、この発明によれば、餌料槽１２
から培養槽１４へ送りこまれる新鮮な餌料混入
過水が培養槽１４内におけるワムシの代謝物また
は排泄物、あるいは増殖する細菌類などの水質を
悪化させる原因となるものを連続的に希釈し、そ
の後、ワムシ収獲ポンプ２０によつて収獲槽１６
側へ抜きとられてゆくので、相当高密度で、しか
も連続したワムシの培養が可能である。 On the contrary, according to the present invention, the feed tank 12
The fresh feed-containing superwater fed into the culture tank 14 continuously dilutes rotifer metabolites or excreta in the culture tank 14, as well as bacteria that proliferate and other substances that cause deterioration of water quality. , a harvesting tank 16 by a rotifer harvesting pump 20.
Since the rotifers are pulled out to the side, it is possible to culture rotifers at a fairly high density and continuously.
しかし、水質をより一層清浄に保つためには、
前記の希釈および抜きとりの率に釣り合う増殖率
を有する品種のワムシ株、たとえばタイ国産のＳ
型ワムシなどを選択することが望ましい。一般に
は水質との兼合いから、ワムシの比増殖率が１を
超えることが目安となる。 However, in order to keep the water quality even cleaner,
A rotifer strain of a variety having a growth rate commensurate with the above-mentioned dilution and sampling rates, such as S.
It is preferable to choose rotifers. Generally speaking, the specific growth rate of rotifers should be more than 1 in consideration of water quality.
さて、以上に述べた内容からして、例えば毎日
午前10時から翌日の午前９時に至る23時間稼動す
る場合、培養液100リツトルに対して餌料100リツ
トルを定量的に注入し、また同時に培養槽１４か
ら100リツトルを定量的に収獲する。このような
収獲において、培養槽１４内にワムシの密度を常
に一定、たとえば500個体／mlに維持することに
より、その収獲量は１日当り
500個体／ml×100＝5000万個体
を確保することができる。 Now, based on the above, when operating for 23 hours from 10 a.m. to 9 a.m. the next day, for example, 100 liters of feed should be quantitatively injected into the culture tank and 100 liters of culture solution should be injected into the culture tank at the same time. Quantitative harvest of 14 to 100 liters. In such harvesting, by always maintaining the density of rotifers in the culture tank 14 at a constant constant, for example, 500 individuals/ml, the harvest amount can be secured at 500 individuals/ml x 100 = 50 million individuals per day. can.
この発明のワムシの連続培養法は、以上に詳述
した技術的な内容から自明であるように、先ず最
初に、海水を海水過装置１０によつて過して
ワムシの増殖培養に不適切な生物その他を排除
し、その過された海水を餌料槽１２および培養
槽１４にそれぞれ供給しておく。
As is obvious from the technical content detailed above, the continuous rotifer culture method of the present invention first passes seawater through the seawater filtration device 10 to remove water that is unsuitable for the growth and culture of rotifers. Organisms and other organisms are removed, and the filtered seawater is supplied to a feed tank 12 and a culture tank 14, respectively.
次で、連続培養操作して、餌料槽１２からワム
シ培養槽１４への餌料供給速度と、培養槽１４か
らワムシの収獲速度とを等しくして、連続的に給
餌し、かつ連続的に収獲をおこなう。 Next, a continuous culture operation is performed to equalize the rate of feed supply from the feed tank 12 to the rotifer culture tank 14 and the rate of harvest of rotifers from the culture tank 14 to continuously feed and harvest rotifers. Let's do it.
このように培養槽１４におけるワムシの増殖分
を常に定量、収獲すなわち取り出すことによつ
て、培養槽１４内のワムシの個体数は常時一定に
維持される。 By constantly quantifying, harvesting, or taking out the amount of rotifer growth in the culture tank 14 in this manner, the population of rotifers in the culture tank 14 is maintained constant at all times.
このようにすることによつて、ワムシ培養に当
つて、水質の管理を特に必要とすることなく、ま
た特に専門的な技術を要することなしに、設備施
設全体を機械化して大量の培養を行うことができ
る。 By doing this, when cultivating rotifers, the entire facility can be mechanized and a large amount of culture can be carried out without the need for particular water quality control or the need for particularly specialized techniques. be able to.
このことは施設の利用を効率化し、全体の種苗
生産の能率を高めると共に、作業員を省力化し、
経済的な面での効果を極めて高いものである。 This makes the use of facilities more efficient, increases the efficiency of overall seedling production, and saves labor for workers.
It has extremely high economic effects.
添付図面はこの発明によるワムシの連続培養法
を実施する設備の概要を説明する略図である。図
面における主な参照数字を挙げると、次のとおり
である。
１０……海水過装置、１２……餌料槽、１４
……培養槽、１６……収獲槽、１８，２０……ポ
ンプ、２６……撹拌機、２８……エアストーン装
置、３０……ヒーター、３２……温度センサ。
The accompanying drawings are schematic diagrams illustrating the outline of equipment for carrying out the continuous rotifer culture method according to the present invention. The main reference numbers in the drawings are as follows. 10...Seawater filtration device, 12...Food tank, 14
... Culture tank, 16 ... Harvesting tank, 18, 20 ... Pump, 26 ... Stirrer, 28 ... Air stone device, 30 ... Heater, 32 ... Temperature sensor.
Claims (1)
水中の他の微小動物を除去することと、 前記濾過水にワムシの飼料としてナンノクロロ
プシス、ビタミンB12および淡水クロレラを混入
することと、 前記飼料混入濾過水をワムシの培養槽に供給す
ることと、 前記培養槽内の水温を所定の温度に維持するこ
とと、 前記培養槽へ供給する前記飼料の量と前記培養
槽内の水量とを一定に制御することと、 前記培養槽に供給する飼料混入濾過水の供給速
度と同一速度で前記培養槽からワムシを収穫する
こととから成るワムシの連続培養法。[Claims] 1. Microfiltration of seawater to remove other microscopic animals in the seawater that compete with rotifers for food; and adding Nannochloropsis, vitamin B12, and freshwater chlorella to the filtered water as feed for the rotifers. supplying the filtered water mixed with the feed to a rotifer culture tank; maintaining the water temperature in the culture tank at a predetermined temperature; and controlling the amount of the feed to be supplied to the culture tank and the culturing tank. A continuous cultivation method for rotifers comprising: controlling the amount of water in the tank to a constant level; and harvesting rotifers from the culture tank at the same rate as the feed rate of filtered water mixed with feed supplied to the culture tank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1174285A JPH0339021A (en) | 1989-07-07 | 1989-07-07 | Continuous culture of rotifer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1174285A JPH0339021A (en) | 1989-07-07 | 1989-07-07 | Continuous culture of rotifer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0339021A JPH0339021A (en) | 1991-02-20 |
| JPH0475735B2 true JPH0475735B2 (en) | 1992-12-01 |
Family
ID=15976001
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1174285A Granted JPH0339021A (en) | 1989-07-07 | 1989-07-07 | Continuous culture of rotifer |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0339021A (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2594204B2 (en) * | 1992-05-06 | 1997-03-26 | 社団法人マリノフォーラム二十一 | Water purification system for zooplankton culture tank |
| JP2005261342A (en) * | 2004-03-19 | 2005-09-29 | Yanmar Co Ltd | Plankton culture system |
| JP4953118B2 (en) * | 2005-07-14 | 2012-06-13 | エーケイブイエー グループ デンマーク エイ／エス | Aquaculture system for producing aquaculture fish using seawater fish feeding sources including amphipods |
| CN111528147A (en) * | 2020-05-21 | 2020-08-14 | 中国水产科学研究院东海水产研究所 | Small water body high-density rotifer cultivation method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS4832696A (en) * | 1971-08-17 | 1973-05-01 | ||
| JPH01112933A (en) * | 1987-10-27 | 1989-05-01 | Yakult Honsha Co Ltd | Method for cultivating brachionus plicatilis o.f. muller |
-
1989
- 1989-07-07 JP JP1174285A patent/JPH0339021A/en active Granted
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS4832696A (en) * | 1971-08-17 | 1973-05-01 | ||
| JPH01112933A (en) * | 1987-10-27 | 1989-05-01 | Yakult Honsha Co Ltd | Method for cultivating brachionus plicatilis o.f. muller |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0339021A (en) | 1991-02-20 |
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