JPH0462341B2 - - Google Patents
Info
- Publication number
- JPH0462341B2 JPH0462341B2 JP60085275A JP8527585A JPH0462341B2 JP H0462341 B2 JPH0462341 B2 JP H0462341B2 JP 60085275 A JP60085275 A JP 60085275A JP 8527585 A JP8527585 A JP 8527585A JP H0462341 B2 JPH0462341 B2 JP H0462341B2
- Authority
- JP
- Japan
- Prior art keywords
- separation
- development
- autoradiograph
- determining
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000926 separation method Methods 0.000 claims description 70
- 238000011161 development Methods 0.000 claims description 56
- 150000007523 nucleic acids Chemical class 0.000 claims description 43
- 108020004707 nucleic acids Proteins 0.000 claims description 42
- 102000039446 nucleic acids Human genes 0.000 claims description 42
- 239000012634 fragment Substances 0.000 claims description 39
- 238000000034 method Methods 0.000 claims description 39
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 32
- 238000003672 processing method Methods 0.000 claims description 25
- 239000000126 substance Substances 0.000 claims description 22
- 239000000203 mixture Substances 0.000 claims description 18
- 238000012545 processing Methods 0.000 claims description 14
- 239000000463 material Substances 0.000 claims description 11
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims description 10
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 claims description 10
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims description 8
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims description 5
- 229930024421 Adenine Natural products 0.000 claims description 5
- 229960000643 adenine Drugs 0.000 claims description 5
- 229940104302 cytosine Drugs 0.000 claims description 5
- 238000003491 array Methods 0.000 claims description 4
- 229940113082 thymine Drugs 0.000 claims description 4
- 230000005284 excitation Effects 0.000 claims description 3
- 230000001678 irradiating effect Effects 0.000 claims description 3
- 238000012163 sequencing technique Methods 0.000 claims 2
- 238000013508 migration Methods 0.000 description 28
- 230000005012 migration Effects 0.000 description 28
- 230000005855 radiation Effects 0.000 description 16
- 238000001962 electrophoresis Methods 0.000 description 12
- 230000002285 radioactive effect Effects 0.000 description 8
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 6
- 238000012937 correction Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000003776 cleavage reaction Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 230000007017 scission Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000007018 DNA scission Effects 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 238000000376 autoradiography Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 239000007857 degradation product Substances 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000002601 radiography Methods 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- 229910052693 Europium Inorganic materials 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- -1 europium-activated barium fluoride bromide Chemical class 0.000 description 1
- 230000017525 heat dissipation Effects 0.000 description 1
- 238000007373 indentation Methods 0.000 description 1
- 230000010365 information processing Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000004816 paper chromatography Methods 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000000163 radioactive labelling Methods 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
Landscapes
- Measurement Of Radiation (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60085275A JPS61243361A (ja) | 1985-04-19 | 1985-04-19 | 核酸の塩基配列決定のための信号処理方法 |
| US06/854,381 US4720786A (en) | 1985-04-19 | 1986-04-21 | Method of compensating for offset distortion in rows of electrophoretic patterns |
| EP86105493A EP0199327B1 (fr) | 1985-04-19 | 1986-04-21 | Méthode de traitement de signaux pour déterminer l'ordre des bases de l'acide nucléique |
| DE8686105493T DE3684030D1 (de) | 1985-04-19 | 1986-04-21 | Signalverarbeitungsverfahren um die reihenfolge der basen von nukleinsaeure festzustellen. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60085275A JPS61243361A (ja) | 1985-04-19 | 1985-04-19 | 核酸の塩基配列決定のための信号処理方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61243361A JPS61243361A (ja) | 1986-10-29 |
| JPH0462341B2 true JPH0462341B2 (fr) | 1992-10-06 |
Family
ID=13854009
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60085275A Granted JPS61243361A (ja) | 1985-04-19 | 1985-04-19 | 核酸の塩基配列決定のための信号処理方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61243361A (fr) |
-
1985
- 1985-04-19 JP JP60085275A patent/JPS61243361A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS61243361A (ja) | 1986-10-29 |
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