JPH045543A - Reaction measuring instrument - Google Patents

Abstract

PURPOSE:To prevent the connection part between both a piezoelectric element fitted with a cell and a device main body from deteriorating in electric characteristics by providing the connection part which employs inductance coupling between the piezoelectric element fitted with the cell and an oscillation circuit. CONSTITUTION:The piezoelectric element 1 is fitted with the cell 2. A coil 13 which is connected to the piezoelectric element 1 is fixed on the flank or bottom surface of the cell 2, and the piezoelectric element 1 and oscillation circuit part 3 are connected by the inductance coupling with a coil 14 which is connected to the oscillation circuit and fixed at the installation part of the piezoelectric element 1. The oscillation circuit 3 is connected to a frequency counter 4 and further connected to a microcomputer 5 for monitoring data, and a recording device 6, a display device 7 and a key switch 8 are connected to the microcomputer 5. Therefore, the connection terminals of both the piezoelectric element fitted with the cell and the device main body need not be exposed out and even when the connection terminals are exposed out, the surfaces of the connection terminals are so processed as to improve the wear resistance and anticorrosiveness.

Description

[Detailed Description of the Invention] [Field of Industrial Application] The present invention is applicable to chemistry, physical chemistry, biochemistry, polymer chemistry, and medical, pharmaceutical, food, and chemical industries, tracking of chemical reactions, immune reactions, and physical property analysis. , and a device that performs measurements using it.

(Summary of the Invention) The present invention provides a reaction measuring device using a piezoelectric element as a detector, in which an electrical connection is established between the piezoelectric element and an oscillation circuit, an impedance measuring device, an impedance-resonant frequency measuring device, or a signal switching circuit by inductance coupling. By providing a connecting portion, the connecting portions of both the piezoelectric element with the cell and the main body of the device are not exposed to the outside.

Further, even when the connecting portion is exposed to the outside, the surface of the connecting portion can be treated to improve wear resistance and corrosion resistance. This prevents the electrical characteristics of the connection part from deteriorating during operations such as cleaning and heat treatment of the piezoelectric element with the cell attached, or due to the physical or chemical effects of samples, water, etc. It has become possible to improve the operability of attaching and detaching the attached piezoelectric element to the main body of the device.

[Conventional technology]

The reaction measuring device of the present invention can mainly measure reactions accompanied by viscosity changes. Conventionally, the capillary method, rotation method, falling ball method, etc. have been used to measure viscosity. The capillary method determines the viscosity from the rate at which the sample solution falls through a capillary, and the falling ball method involves placing a metal ball in the sample solution and determining the viscosity from the rate at which it falls. In the rotation method, viscosity is determined by rotating a cylindrical metal rod in a sample solution and determining shear stress. On the other hand, when the target of reaction measurement is a coagulation reaction or gelation reaction, there are methods such as optically measuring the turbidity of the sample, and methods of applying mechanical vibration to the sample and detecting changes in viscosity due to coagulation or gelation. was taken.

[Problem to be solved by the invention]

Conventional viscosity measurement methods have the problem that it takes time to perform a single measurement operation, making it difficult to continuously measure changes caused by a reaction, and also making it impossible to measure small amounts of sample. .

When measuring coagulation reactions or gelation reactions of endotoxins and blood coagulation factors, conventional methods of measuring turbidity
There are problems in that the system is complicated because it includes an optical measurement system, and it is not suitable for measuring colored samples, and depending on the sample, large errors may occur due to salting out. Furthermore, in the method of applying mechanical vibrations, it is difficult to reduce the size and weight of the device due to the presence of mechanical parts, making it inevitable to increase the size of the device.

In addition to these points, each method has the problem of requiring a sample of at least about 0.2 ml.

The measurement method using a piezoelectric element devised by the present inventors was able to measure a sample of 0.2 ml or less. Additionally, since there are no mechanically moving parts, it is easy to downsize the system.

However, until now, there was a direct current connection between the piezoelectric element and the oscillation circuit, so when the piezoelectric element with cells was attached to and detached from the main body of the device, cleaning, heat treatment, etc.
Physical or chemical effects of the sample, oxygen in the air, water, etc. can sometimes deteriorate the electrical characteristics of the connection parts of both the device body and the piezoelectric element attached to the cell.

Furthermore, there is room for improvement in the operability and safety of attaching and detaching the piezoelectric element with the cell to the device body. In particular, when handling samples that are harmful to the human body or have a risk of infection such as viruses, it is a big problem that the connection terminals of piezoelectric elements with cells may damage protective gloves, etc. there were.

In addition, in addition to viscosity measurement, an immune reaction measuring device and a gas sensor system using piezoelectric elements have been devised by the present inventors. Samples and cleaning fluids could remain in small gaps in the cell walls, affecting measurement results, and the liquid in the cell could leak through those small gaps.

A gas sensor system measures the adsorption reaction of gas onto a sensitive film on a quartz crystal resonator. The piezoelectric element fixed inside the cell and the external connection part are connected by a conductor penetrating a part of the wall of the sealed cell, but gas sometimes leaks from the small gap between them.

[Means to solve the problem]

In order to solve these problems, the present invention consists of a piezoelectric element with cells and the same number of oscillation circuits and frequency measurement circuits, a piezoelectric element with cells and an impedance measuring device, or a piezoelectric element with cells and an impedance measuring device. A device consisting of a piezoelectric element with a mark and an impedance resonance frequency measuring device, a device consisting of a data processing control device added to these,
Furthermore, in a reaction measuring device configured by adding a constant temperature chamber to these, or, when using multiple piezoelectric elements, by adding a signal switching circuit to these, a piezoelectric element, an oscillation circuit, an impedance measuring device, an impedance-
An electrical connection part using inductance coupling is provided between the resonance frequency measuring device or the signal switching circuit.

[Effect]

A piezoelectric element is a device that utilizes a piezoelectric effect, and can be caused to oscillate by being connected to an oscillation circuit. At this time, the surface of the piezoelectric element causes minute vibrations. For this reason, it is known that the resonance frequency and resonance resistance of piezoelectric elements change due to the influence of the viscoelasticity and density of the material with which the surface is in contact, as well as changes in weight due to adsorption of materials onto the surface. There is. By measuring this resonance frequency and resonance resistance, it is possible to measure changes in viscoelasticity of liquids and liquid crystals, adsorption of substances, and accumulation of substances due to sinking.

In this reaction measurement device using a piezoelectric element, when a piezoelectric element with a cell is installed in the device body, a conductor connected to the piezoelectric element, an oscillation circuit, an impedance measurement device, an impedance-resonance frequency measurement device, or a signal switching circuit By the inductive coupling of the conductor connected to
If the structure is such that the piezoelectric element and the oscillation circuit, impedance measuring device, impedance resonance frequency measuring device, or signal switching circuit are electrically connected, the connection terminals of both the piezoelectric element with the cell and the main body of the device can be exposed to the outside. There will be no need to do so.

Further, even when the connecting terminal is exposed to the outside, the surface of the connecting terminal can be treated to improve wear resistance and corrosion resistance.

These things prevent deterioration of the electrical characteristics of the connection terminals of both the piezoelectric element with cells and the main body of the device, and improve the operability such as attaching and detaching the piezoelectric element with cells to the main body of the device and cleaning. It also makes it easier to create a sealed part containing the piezoelectric element inside the cell.

〔Example〕

Embodiments of the present invention will be described below based on the drawings. FIG. 1a to FIG. 1n are schematic diagrams of the reaction measuring device of the present invention.

In FIG. 1a, a cell 2 is attached to a piezoelectric element l. A coil 13 connected to the piezoelectric element 1 is fixed to the side or bottom of the cell 2, and the piezoelectric element 1 and the oscillation circuit are connected to the oscillation circuit by inductance coupling with a coil 14 connected to the oscillation circuit and fixed to the installation part of the cell. 3 is connected. The oscillation circuit 3 is connected to a frequency counter 4 and further connected to a microcomputer (including an input/output interface) 5 for monitoring data, and the microcomputer 5 includes a recording device 6, a display device 7, and a key switch. 8 are connected.

In FIG. 1, a cell 2 is attached to a piezoelectric element 1. A coil 13 connected to the piezoelectric element 1 is fixed to the side or bottom of the cell 2, and the piezoelectric element 1 and An impedance measuring device 11 is connected. The impedance measurement bag 211 is connected to a microcomputer (including an input/output interface) 5 for monitoring data, and a recording device 6, a display device 7, and a key switch 8 are connected to the microcomputer 5.

In FIG. 1C, a cell 2 is attached to the piezoelectric element 1. In FIG. A coil 13 connected to the piezoelectric cable 1 is fixed to the side or bottom of the cell 2, and by inductance coupling with a coil 14 connected to the impedance-resonance frequency measuring device 12 and fixed to the installation part of the cell,
Piezoelectric element 1 and impedance-resonance frequency measuring device 12
is connected. The impedance-resonance frequency measuring device 12 is connected to a microcomputer (including an input/output interface) 5 for monitoring data phrases, and a recording device 6, a display device 7, and a key switch 8 are connected to the microcomputer 5. ing.

In FIG. 1d, a cell 2 is attached to the piezoelectric element 1, and the piezoelectric element 1 is installed so as to be in contact with a thermostatic chamber 9.

A coil 13 connected to the piezoelectric element 1 is fixed to the side or bottom of the cell 2, and the piezoelectric element 1 and the oscillation circuit are connected by inductance coupling with a coil 14 connected to the oscillation circuit and fixed to the installation part of the cell. 3 is connected. The oscillation circuit 3 is connected to a frequency counter 4, and further includes a microcomputer (
5 (including an input/output interface), and a recording device 6, a display device 7, and a key switch 8 are connected to the microcomputer 5.

In FIG. 1e, a cell 2 is attached to the piezoelectric element 1, and the piezoelectric element 1 is installed so as to be in contact with a thermostatic chamber 9.

A coil 13 connected to the piezoelectric element 1 is fixed to the side or bottom of the cell 2, and the impedance measuring device 11
The piezoelectric element 1 and the impedance measuring device 11 are connected by inductance coupling with a coil 14 connected to and fixed to the installation part of the cell. The impedance measurement device 8 and 11 are connected to a microcomputer (including an input/output interface) 5 for monitoring data, and the microcomputer 5 includes a recording device 6,
A display device 7 and a key switch 8 are connected.

In FIG. 1f, a cell 2 is attached to the piezoelectric element 1, and the piezoelectric element 1 is installed so as to be in contact with a thermostatic chamber 9.

A coil 13 connected to the piezoelectric element 1 is fixed to the side or bottom of the cell 2, and the piezoelectric element is 1
and an impedance resonance frequency measuring device 12 are connected. The impedance-resonance frequency measuring device 12 is connected to a microcomputer (including an input/output interface) 5 for monitoring data, and a recording device 6, a display device 7, and a key switch 8 are connected to the microcomputer 5. ing.

In Fig. 1g, piezoelectric elements 1+, 1t+...l
, l are attached with cells 21.2□, . . . , 211. Coils 13+, 13z are connected to the piezoelectric elements 1+, 1g, . . . 1, l on the side or bottom surfaces of the cells 2+, 2g, .

..., 137 is fixed, coils 141, 142, ..., which are connected to the oscillation circuit and fixed to the installation part of the cell.
147, the piezoelectric element 1.
．． 1! , ..., 1.1 and the oscillation circuit 33, 3□, .
..., 3R is connected.

The third oscillation circuit 31, 3□, . A recording device 6, a display device 7, and a key switch 8 are connected to the microcomputer 5.

In the first diagram, piezoelectric element II, 1g, . . . 1
, contains cell 2. ．． 2g, ..., 2. is attached. On the side or bottom surfaces of the cells L, 2t, . . . , 2n, coils 13 . ．． 13□.

..., 13, are fixed, and coils 14+, 14 are connected to the signal switching circuit IO and fixed to the installation part of the cell.
□,...,14. The signal switching circuit 10 is connected to the piezoelectric elements 11, 1□, .

The signal switching circuit 10 includes an impedance measurement device 11
and further connected to a microcomputer (including an input/output interface) 5 for monitoring data, and the microcomputer 5 has a recording device 6.
, a display device 7, and a key switch 8 are connected.

In FIG. 1i, piezoelectric elements 1+, I□, . . . 1 have cells 2. ．． 2□,...,2. It is marked with an l. Cells 2+, 2g, ..., 2RO) Coils 131.13t connected to piezoelectric elements II, 1□, ...1, l are on the side or bottom.

..., ] 3□ is fixed, the coil 141.14 is connected to the signal switching circuit 10 and fixed to the installation part of the cell.
．． , ..., 14. The signal switching circuit 10 is connected to the piezoelectric elements II, 1□, . . .

The signal switching circuit 10 is connected to an impedance-resonance frequency measuring device 12 and further connected to a microcomputer (including an input/output interface) 5 for monitoring data. A device 7 and a key switch 8 are connected.

In FIG. 1j, piezoelectric elements 1+, lx, . . . 1
, cell 2. ．． 2. , ..., 2. , and is installed so as to be in contact with the thermostatic chamber 9.

Cells 21, 22. . . , 2n has piezoelectric elements 11 . Iz, ..., 1. The coils 131.13g, . . . , 137 connected to the oscillator circuit are fixed, and the piezoelectric Element 1+, 1g, ...
, 1. 0 oscillation circuit 31+ 31. l and oscillation circuits 3I, 3g, . . . , 3 are connected. ..., 3.
is connected to the frequency counter 4 via the tll engineer ZIaLO which is connected to the frequency counter 4, and is further connected to a microcomputer (including an input/output interface) 5 for monitoring data, and the microcomputer 5 has a recording device. 6, a display device 7, and a key switch 8 are connected.

In the engineering drawing k, piezoelectric elements 1+, Iz,...1.1
Cells 21, 2t, .

Piezoelectric elements 1+, lz, . . . , 1. , coils 13+, 13□, . ．． 14□..., 1411, the piezoelectric elements 13, 1□, .
..., 1n and the signal switching circuit 10 are connected.

The signal switching circuit 10 includes an impedance measurement device 11
microcomputer (including input/output interface) for further data monitoring
A recording device 6, a display device 7, and a key switch 8 are connected to the microcomputer 5.

1st V! At J+, piezoelectric elements 11, 1□, ... 1
, l is attached with cells 2++2t,..., 2.1, and is kept at a constant temperature! It is installed so as to be in contact with S9.

On the sides and bottom of the cells 28, 2□, ..., 2,
Piezoelectric elements 1+, 1z,..., 1. Coil 13 connected to ．． 13g, . . . , 13.1 are fixed, and the coil 14.1 is connected to the signal switching circuit 10 and fixed to the installation part of the cell. ．． 14! ... 14. By inductance coupling with l, piezoelectric element 15.1t, ・
..., lo and the signal switching circuit 10 are connected.

The signal switching circuit 10 is connected to an impedance-resonance frequency measuring device 12 and further connected to a microcomputer (including an input/output interface) 5 for monitoring data. A device 7 and a key switch 8 are connected.

In Fig. 1m, the piezoelectric element 1 on which the antibody was immobilized was placed in contact with the sample on both sides, and was sealed except for the two inlet/outlet ports for introducing and discharging liquid into the cell. It is installed in cell 2. Cell 2 is thermostat 9
It is installed so that it is in contact with. A coil 13 connected to the piezoelectric element 1 is fixed to the inner wall of the cell 2, and the piezoelectric element 1 and the oscillation circuit 3 are connected to each other by inductance coupling with a coil 14 connected to the oscillation circuit and fixed to the installation part of the cell. It is connected. The oscillation circuit 3 is connected to a frequency counter 4, and further includes a microcomputer (including an input/output interface) 5 for monitoring data.
A recording device 6, a display device 7, and a key switch 8 are connected to the microcomputer 5.

In FIG. 1n, a piezoelectric element 11.1 coated with a sensitive membrane
□ is installed in a sealed cell 2 with a rubber part 17 for introducing gas or easily vaporized liquid into the cell. The cell 2 is placed in contact with a thermostat 9. Coils 13+ and 13g connected to the piezoelectric elements 1+ and 1g are fixed to the inner wall of the cell 2, and a coil 14. ．．
14t, the piezoelectric element 11
．． 1! and the signal switching circuit 10 are connected. The signal switching circuit 10 is connected to an impedance resonance frequency measuring device 12 and further connected to a microcomputer (including an input/output interface) 5 for monitoring data, and the microcomputer 5 includes a recording device 6 and a display device. 7. Key switch 8 is connected. Further, the crystal resonator and the cell are integrated, and the crystal resonator cell can be easily removed from the main body of the measuring device, and operations such as cleaning can be performed, and the cell can be made disposable.

[Application Example 1 to Measurement] In this example, an application to endotoxin analysis will be described. A crystal resonator cell (piezoelectric element with a cell) using 16 9MHz, AT-cut crystal resonators,
A constant temperature chamber was installed in this reaction measuring device at 37° C., and 0.2 ml of endotoxin sample solution and a specified amount of freeze-dried horseshoe crab blood cell extract were mixed and injected into a quartz crystal cell.

This operation was performed sequentially on 16 crystal resonators, and at the same time, the gelation time (the time until the oscillation frequency stopped changing) was measured.

Calculation of the gelation time was performed in the data processing section, and the gelation time varied depending on the endotoxin concentration between 4-10-'EU-m1-1. Furthermore, measurement was possible even with a sample amount of 20 μl.

During these measurements, there are no externally exposed connection terminals on either the crystal resonator cell or the device body, and there is no need to make physical contact between the device body and the connection terminals of the piezoelectric element attached to the cell. Operations such as attaching and detaching the vibrator cell and the main body of the device could be performed easily and quickly. moreover,
There was no damage to protective gloves, etc., caused by the connection terminal during operation.

After the measurement, the crystal resonator cell was washed with water, alcohol, and acid, and was further subjected to dry heat treatment at 180° C. for 4 hours, but the electrical characteristics of the connected portion did not deteriorate.

[Application Example 2 to Measurement] In this example, an application to a blood coagulation test will be described. A crystal resonator cell using one, six, or eight 9MHz, AT-cut crystal resonators is heated in a thermostat at 37°C.
A factor 8-deficient blood sample was placed in this reaction measuring device at 0.9°C and incubated for 1 minute in advance. Q5ml, activated partial thromboplastin solution 0.05ml, sample 0.0
After adding 5 ml and incubating for a further 2 minutes,
0.05 ml of 0.025M calcium chloride solution was added and immediately poured into the quartz crystal cell. When measuring multiple samples simultaneously, this operation was performed sequentially for 6 or 8 crystal units, and the coagulation time (the time until the oscillation frequency stopped changing) was measured at the same time. The coagulation time varied depending on the factor 8 concentration between 110-"UNIT-ml-'. Furthermore, measurement was possible even with a sample volume of 20 μl.

During these measurements, there is no externally exposed Vt terminal on either the crystal resonator cell or the device body, and there is no need to mechanically contact the device body with the connection terminal of the piezoelectric element attached to the cell. Operations such as attaching and detaching the crystal resonator cell and the main body of the device could be performed easily and quickly. Furthermore, since the connection terminal does not damage protective gloves or the like during operation, operability and safety do not deteriorate even when measuring samples such as blood products and heated human plasma.

After the measurement, the quartz crystal resonator cell was washed with water, alcohol, and acid, and dried, but the electrical characteristics of the connected parts did not deteriorate.

Further, in this example, measurement of factor 8 was described as a test item, but good results were also obtained for test items of other blood coagulation factors such as factor 9 and fifurinogen.

[Application Example 3 to Measurement] In this example, an application to viscosity measurement will be described. A crystal resonator cell using one 9 MHz, AT-cut crystal resonator was installed in this reaction measuring device, a sample liquid was injected into the crystal resonator cell, and the oscillation frequency was measured. The difference between this oscillation frequency and the oscillation frequency determined when there is no sample liquid corresponds well to the viscosity measured with other viscometers in water-methanol systems, water-ethanol systems, water-glycerin systems, etc. showed the relationship.

During these measurements, there are no externally exposed connection terminals on either the crystal resonator cell or the device body, and there is no need to mechanically contact the connection terminals between the device body and the crystal resonator cell. Operations such as attaching and detaching the cell and the main body of the device can be performed easily and quickly, and the electrical characteristics of the connected parts did not deteriorate even when measurements were continued for a long time under hot and humid conditions. .

[Application Example 4 to Measurement] In this example, an application to immunoassay will be described. The FIB3 antibody was immobilized on a 9 MHz AT cant crystal oscillator, and the quartz crystal oscillator cell integrated with the cell was installed in this reaction measuring device, and the resonance frequency was measured while flowing pure water through the quartz crystal oscillator cell. After that, the CEA antigen sample solution was introduced into the crystal oscillator cell, and after reacting, the resonance frequency was measured while flowing pure water again.゜3X
It varied depending on the concentration between 10' ng-m and 1.

In this example, measurements are taken while flowing pure water into the cell, but the crystal oscillator and oscillation circuit are composed of two coils placed across the cell wall, as shown in Figure 1. Since the connections were made through inductance coupling, there was no possibility that the sample or pure water would leak from the cell, or that the sample would remain in the small gap between the cell and the connection terminal, which would affect the measurement results.

[Application Example 5 to Measurement] In this example, an application to a gas identification sensor system will be described. Two 9MHz, AT-cut crystal oscillators are used, one crystal oscillator has an azo lectin membrane,
Another quartz crystal oscillator was coated with a 3:1 mixed film of azolectin/cholesterol, and the quartz crystal oscillator cell integrated with the cell was installed in this reaction measuring device. Thereafter, an odorant gas was introduced into the cell and measurements were taken.

Measurement targets include β-1onone, methano
l, ethanol, citral, acetone,
ethyl ether was used.

Using this reaction measurement device, we determined the ratio of the change in resonance resistance and resonance frequency when a gas was adsorbed to the sensitive film on the crystal oscillator, and found that it varied depending on the type of gas, making it possible to identify the gas.

The crystal oscillator and impedance-resonance frequency measurement device is
As shown in Figure 1n, two
Since the two coils were connected by inductive coupling, the measurement results were not affected by gas leakage during the measurement.

Furthermore, this reaction measuring device was also able to measure gas concentration from the amount of change in resonance resistance or resonance frequency.

〔Effect of the invention〕

As explained above, the reaction measurement device of the present invention provides an electrical connection between the piezoelectric element and the oscillation circuit, the impedance measurement device, the impedance-resonance frequency measurement device, or the signal switching circuit by inductance coupling. This eliminates the need to expose the connection terminals of both the piezoelectric element and the main body of the device to the outside, and even if the connection part is exposed to the outside, the surface of the connection part is treated to improve wear resistance and corrosion resistance. It is designed so that it can be applied. For this purpose, the piezoelectric element with cells may be damaged during operations such as attaching and detaching the piezoelectric element with cells to the main body of the device, cleaning, heat treatment, etc., or due to physical or chemical effects such as oxygen or water in the sample or air. It was possible to prevent deterioration of the electrical characteristics of the connection parts of both the device and the main body of the device.

Furthermore, since there is no need to physically contact the connection terminals of the device body and the piezoelectric element with cells, the operability and safety of attaching and detaching the device body and the piezoelectric device with cells are improved. In particular, the possibility of damaging the connecting terminal of the piezoelectric element with a cell, or protective gloves etc. when attaching and detaching the piezoelectric element with a cell to the main body of the device has been greatly reduced, so it is less likely to be harmful to the human body. The effect is remarkable when handling samples that are at risk of infection, such as viruses.

Furthermore, since the operations for attaching and detaching have become extremely simple, it is now possible to automate cell exchange, creating a system that automates all operations from sample dispensing to cell exchange. is now possible.

Furthermore, when it was necessary to seal the part of the cell into which the sample was introduced, including the piezoelectric element, this could be easily accomplished.

[Brief explanation of the drawing]

FIG. 1a to FIG. 1n show schematic diagrams of other embodiments of the reaction measuring device of the present invention. 1 and II,..., 1. - piezoelectric elements 2 and 21.
..., 211--cells 3 and 31. ...,
311--Oscillation circuit 4--Frequency counter 5-Computer 6--Recording device 7-Display device 8-Key switch 9-Thermostat 10-Signal switching circuit 11-Impedance measurement device 12-Impedance-Resonance frequency measurement device 13 and 13. ．． 13□,...,i3. l·-coil 14 and 141.14□, . . . , 14. l-Coil 15-Rubber and above Applicant: Seiko Electronic Industries Co., Ltd. Agent Patent Attorney: Keisuke Hayashi Figure 9 Figure d Figure f

Claims (14)

[Claims] (1) A reaction measurement device comprising a piezoelectric element with a cell, an oscillation circuit, and a frequency measurement circuit, characterized in that the reaction measurement device has a connection part by inductance coupling between the piezoelectric element and the oscillation circuit. (2) A reaction measuring device comprising a piezoelectric element with a cell and an impedance measuring device, the reaction measuring device having a connection part by inductance coupling between the piezoelectric element and the impedance measuring device. (3) A reaction measurement device consisting of a piezoelectric element with a cell and an impedance-resonance frequency measuring device, which is characterized by having a connection part by inductance coupling between the piezoelectric element and the impedance-resonant frequency measuring device. Device. (4) In a reaction measurement device consisting of a piezoelectric element with multiple cells, the same number of oscillation circuits, a signal switching circuit, and a frequency measurement circuit, a connection section using inductance coupling is used between the piezoelectric element and the oscillation circuit. A reaction measuring device comprising: (5) A reaction measuring device comprising a piezoelectric element with a plurality of cells, a signal switching circuit, and an impedance measuring device, which is characterized by having a connection part by inductance coupling between the piezoelectric element and the signal switching circuit. Measuring device. (6) A reaction measuring device consisting of a piezoelectric element with a plurality of cells, a signal switching circuit, and an impedance-resonance frequency measuring device, characterized by having a connection part by inductance coupling between the piezoelectric element and the signal switching circuit. A reaction measuring device. (7) The reaction measuring device according to any one of items 1 to 6, characterized in that it has a configuration in which a data processing control device is added to the reaction measuring device configured as described above. (8) The reaction measuring device according to any one of items 1 to 7, characterized in that it has a configuration in which a thermostat is added to the reaction measuring device having the above configuration. (9) The inductance coupling connection portion connects the coil connected to the piezoelectric element and the coil connected to the oscillation circuit, the impedance measurement device, the impedance-resonance frequency measurement device, or the signal switching circuit. 9. The reaction measuring device according to any one of items 1 to 8, characterized in that the device is configured by inductance coupling. (10) The reaction measuring device according to any one of items 1 to 9, wherein the piezoelectric element with the cell is removable from the device main body. (11) The coil connected to the piezoelectric element,
The coil, which forms part of a cell and is connected to the oscillation circuit, the impedance measurement device, the impedance-resonance frequency measurement device, or the signal switching circuit, forms part of the main body of the device. The reaction measuring device according to any one of items 1 to 10, characterized in that: (12) The reaction measuring device according to any one of items 1 to 11, wherein the piezoelectric element is an AT cut crystal resonator or a BT cut crystal resonator. (13) The reaction measurement according to any one of paragraphs 1 to 12, wherein the data processing control device is comprised of a microcomputer, a key input switch, a display device, a recording device, and an input/output signal interface. Device. (14) The reaction measuring device according to any one of items 1 to 13, wherein the piezoelectric element is attached to a cell so that only one electrode is in contact with the sample.