JPH0452113B2 - - Google Patents
Info
- Publication number
- JPH0452113B2 JPH0452113B2 JP59146814A JP14681484A JPH0452113B2 JP H0452113 B2 JPH0452113 B2 JP H0452113B2 JP 59146814 A JP59146814 A JP 59146814A JP 14681484 A JP14681484 A JP 14681484A JP H0452113 B2 JPH0452113 B2 JP H0452113B2
- Authority
- JP
- Japan
- Prior art keywords
- culture
- section
- cell
- animal cells
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 210000004102 animal cell Anatomy 0.000 claims description 43
- 238000004113 cell culture Methods 0.000 claims description 34
- 230000002503 metabolic effect Effects 0.000 claims description 17
- 238000012544 monitoring process Methods 0.000 claims description 16
- 239000001963 growth medium Substances 0.000 claims description 12
- 239000002609 medium Substances 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 8
- 238000000926 separation method Methods 0.000 claims description 8
- 210000004027 cell Anatomy 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 239000011491 glass wool Substances 0.000 claims description 4
- 239000000969 carrier Substances 0.000 claims 1
- 239000000126 substance Substances 0.000 description 7
- 239000003513 alkali Substances 0.000 description 5
- 230000007613 environmental effect Effects 0.000 description 5
- 239000007789 gas Substances 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- 230000035939 shock Effects 0.000 description 4
- 230000012010 growth Effects 0.000 description 3
- 238000009629 microbiological culture Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 230000001766 physiological effect Effects 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 230000001464 adherent effect Effects 0.000 description 2
- 238000013019 agitation Methods 0.000 description 2
- 239000012737 fresh medium Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- UBAZGMLMVVQSCD-UHFFFAOYSA-N carbon dioxide;molecular oxygen Chemical compound O=O.O=C=O UBAZGMLMVVQSCD-UHFFFAOYSA-N 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 210000001840 diploid cell Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14681484A JPS6125483A (ja) | 1984-07-17 | 1984-07-17 | 細胞の培養方法および装置 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14681484A JPS6125483A (ja) | 1984-07-17 | 1984-07-17 | 細胞の培養方法および装置 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6125483A JPS6125483A (ja) | 1986-02-04 |
| JPH0452113B2 true JPH0452113B2 (esLanguage) | 1992-08-20 |
Family
ID=15416129
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP14681484A Granted JPS6125483A (ja) | 1984-07-17 | 1984-07-17 | 細胞の培養方法および装置 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6125483A (esLanguage) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6314687A (ja) * | 1986-07-04 | 1988-01-21 | Marubishi Baioenji:Kk | 培養装置の気液接触装置 |
| JP3060384B2 (ja) * | 1988-10-27 | 2000-07-10 | 雪印乳業株式会社 | 吸着カラムを連結させた細胞培養法およびそのための装置 |
| JP2505355Y2 (ja) * | 1988-11-09 | 1996-07-31 | 日本特殊陶業株式会社 | スロ―アウェイチップ |
| US7239394B2 (en) | 2003-06-04 | 2007-07-03 | Inverness Medical Switzerland Gmbh | Early determination of assay results |
-
1984
- 1984-07-17 JP JP14681484A patent/JPS6125483A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6125483A (ja) | 1986-02-04 |
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