JPH0451600Y2 - - Google Patents

Description

[Detailed description of the invention] [Field of industrial application] This invention is a biological reaction used in experiments on biological reaction treatments such as production of amino acids and alcohol using enzymes and microorganisms, and purification of organic wastewater. Regarding separation experimental equipment.

[Prior Art] Conventionally, a biological reaction separation device (MB:
Membrane Bioreacter). This MB is equipped with a reaction device for obtaining reactions by enzymes and microorganisms, and a separation device for extracting only the reaction treatment liquid without leaking enzymes or microorganisms. Further, the separation device is equipped with an ultrafiltration membrane, and the concentrated liquid accumulated on the upstream side of the membrane is returned to the reaction device by a reflux device.

On the other hand, when performing the treatment using MB as described above, it is necessary to conduct various experiments prior to implementation, for example, in order to confirm and research the performance of the membrane, the reaction state, etc. Conventionally, such experiments
It is customary to use experimental equipment that is a scaled-down version of the actual machine.

[Problems that the invention attempts to solve] However, an experimental device that is a scaled-down version of the actual device is inevitably equipped with a reaction device, a separation device, and a reflux device, making the device complicated and difficult to use as an experimental device. There is a problem that it tends to become large. This not only increases the cost of the equipment but also requires the preparation of a large amount of samples, which is a major hindrance to simple experiments.

The present invention was made in view of the above-mentioned current situation, and aims to provide a simple biological reaction experimental device that enables easy experiments.

[Means for Solving the Problems] The means taken to achieve the above object in the present invention will be explained with reference to FIG. 1, which corresponds to an embodiment of the present invention. A reaction vessel 1 is divided into upper and lower parts by a membrane 2, an outflow pipe 3 is connected to the upper part, an inflow pipe 4 is connected to the lower part, and a stirring blade 5 that is rotated horizontally below the membrane 2 is installed in the reaction vessel 1. It is intended to be a reaction separation experimental device.

[Function] According to the present invention, the reaction vessel 1 functions as both a reaction device and a separation device, and a reflux device can be omitted. That is, the sample supplied from the inflow pipe 4 is subjected to a reaction at the lower part of the reaction container 1, and
The reaction treatment liquid is passed through the membrane 2 on the spot, flows into the upper part of the reaction vessel 1, and is taken out from there through the outflow pipe 3, so that both reaction and separation are performed within the reaction vessel 1. be exposed. Furthermore, when the treated liquid is taken out, a concentrated liquid is left in the space inside the reaction vessel 1 below the membrane 2, but since this space itself is a reaction field, a reflux device is used to return the concentrated liquid to the reaction apparatus. is not necessary, and experiments can be continued by adding samples. Since one reaction vessel 1 has the function of integrating both a reaction device and a separation device, and has a simple structure without a reflux device, the device can be made small and inexpensive.

On the other hand, if the reaction device and separation device are integrated and the reflux device is omitted, as in this device, the solid content concentration on the surface of the membrane 2 increases as the reaction treatment liquid is taken out, and the permeation capacity of the membrane 2 decreases. It is lost over time, making it impossible to conduct experiments over a long period of time. Therefore, in the present invention, stirring blades 5 are provided. This stirring blade 5 generates turbulent flow on the surface side of the membrane 2,
This prevents the generation of a slime layer that causes the membrane 2 to lose its permeability. Therefore, in the present invention, the good permeation ability of the membrane 2 is maintained for a long time even though the reflux device is omitted.

The stirring blade 5 is designed to be rotated particularly below the membrane 2. Therefore, even if the support of the stirring blade 5 becomes loose, the stirring blade 5 will simply sink downward and separate from the membrane 2, and there is a risk that the stirring blade 5 and the membrane 2 will rub against each other, causing wear and tear on the membrane 2. There is no.

Furthermore, if air bubbles enter the reaction vessel 1 together with the sample and remain there, the effective volume within the reaction vessel 1 will be narrowed and the efficiency will be reduced. However, in this device, since the flow inside the reaction vessel 1 is from bottom to top, air bubbles are transported into the reaction vessel 1 along with this flow.
It is easy to remove from the container, and it is easy to prevent the above-mentioned decrease in efficiency.

[Example] Hereinafter, an example of the present invention will be described in detail with reference to the drawings.

As shown in FIG. 1, the reaction container 1 is constructed by fitting an upper container 1a and a lower container 1b through an annular packing 6, and fixing them with screws 7 and thumbscrews 8. The upper container 1a and the lower container 1b are
Both are made of a transparent material such as synthetic resin or glass, so that the inside can be observed.

As shown in FIGS. 1 and 2, four concentric circular grooves 9a are formed on the ceiling surface of the reaction container 1, that is, the lower surface of the upper container 1a. An outflow pipe 3 is provided passing through the side wall of the upper container 1a, and each of the circular grooves 9a is communicated with the outflow pipe 3 via a straight groove 9b formed in the radial direction.

A circular membrane 2 is arranged to cover these grooves 9a and 9b from below. And this membrane 2
between the peripheral edge of the upper container 1a and the peripheral edge of the ceiling surface of the upper container 1a,
The upper edge of the lower container 1b is fitted into the upper container 1a so as to be sandwiched with an annular packing 6 interposed therebetween.

As shown in FIGS. 1 and 3, below the membrane 2, that is, in the lower container 1b, there is a stirring blade 5 in the shape of a round bar whose diameter is gradually reduced from the center toward both ends.
is placed. This stirring blade 5 is a magnet coated with synthetic resin, and is controlled by a magnetic device 10.
It is simply placed in the lower container 1b and rotated horizontally from the outside of the reaction container 1. As the magnetic device 10, a commercially available magnetic stirrer or the like can be used.

In this way, if the stirring blade 5 is magnetized and rotated by the magnetic device 10 from outside the reaction vessel 1, there is no need to attach a motor or the like to the reaction vessel 1 to rotate the stirring blade 5. There is no. Therefore, it is possible to prevent the reaction vessel 1 from increasing in size due to the attachment of a motor or the like, and from adversely affecting the reaction due to heat generated due to rotation of the motor.
Furthermore, unlike rotation by a motor, there is no need for a shaft to transmit rotation, and rotation can be made by simply placing the magnetic stirring blade 5 in the lower container 1b, so the structure is simple. Just something is enough. However, even in the case of magnetic stirring blade 5, it may be pivoted to stabilize its rotation.

An inflow pipe 4 is passed through the side wall of the lower container 1b, and a sample is supplied to the lower part of the reaction container 1 through the inflow pipe 4. Inflow pipe 4
may be provided in the radial direction of the lower container 1b, but preferably provided in the tangential direction of the rotational circumference of the stirring blade 5. At the same time, if the inflow direction of the sample and the rotation direction of the stirring blade 5 are made the same, the inflow of the sample becomes extremely smooth, and if the rotation direction is reversed, the stirring effect can be increased. Become.

The supplied sample is subjected to a reaction at the lower part of the reaction vessel 1, and is then sucked from the outflow pipe 2 to the membrane 4 and the groove 9a at the upper part of the reaction vessel 1.
9b and is taken out as a reaction treatment liquid.

An air passage 11 is vertically penetrated through the center of the upper surface of the upper container 1a. This functions as an air supply hole when an aerobic reaction is carried out in the reaction vessel 1, and also as an air bubble escape port. In addition, a bottom plate 12 such as a glass plate is placed inside the lower container 1b in order to prevent wear of the bottom surface due to the rotation of the stirring blades 5.

[Effects of the invention] According to the invention, a simple and inexpensive device allows experiments to be carried out easily regardless of the amount of sample, making it easier to conduct research on MB.

[Brief explanation of the drawing]

FIG. 1 is a longitudinal sectional view of an apparatus according to an embodiment of the present invention, FIG. 2 is a bottom view of the upper container, and FIG. 3 is a plan view of the lower container. DESCRIPTION OF SYMBOLS 1... Reaction container, 1a... Upper container, 1b... Lower container, 2... Permeable membrane, 3... Outflow pipe, 4... Inflow pipe, 5... Stirring blade, 6... Packing, 7...
Screw, 8... Thumb screw, 9a, 9b... Groove, 10...
...magnetic device, 11...ventilation path, 12...bottom plate.

Claims (1)

[Scope of utility model registration request] The inside of the reaction vessel is divided into upper and lower parts by a membrane, an outflow pipe is connected to the upper part, and an inflow pipe is connected to the lower part, and a stirring blade that rotates horizontally below the membrane is installed inside the reaction vessel. Characteristic biological reaction separation experimental equipment.